LOCUS       OZ026879               34080 bp    DNA     circular BCT 10-APR-2024
DEFINITION  Escherichia coli isolate B31CTX genome assembly, plasmid:
            pB31CTX-TC-COL-2.
ACCESSION   OZ026879
VERSION     OZ026879.1
DBLINK      BioProject: PRJEB74339
            BioSample: SAMEA115456632
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Doublet,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) INRAE Nouzilly, UMR1282 Infectiologie et
            Sante Publique, Batiment 213, Centre INRAE Val de Loire, 37380
            NOUZILLY, France
FEATURES             Location/Qualifiers
     source          1..34080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="B31CTX"
                     /db_xref="taxon:562"
                     /plasmid="pB31CTX-TC-COL-2"
     gene            1..873
                     /locus_tag="PB31CTX2_1410"
     CDS             1..873
                     /locus_tag="PB31CTX2_1410"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_012599833.1"
                     /note="WP_012599833.1 RepB family plasmid replication
                     initiator protein (Escherichia coli UMN026) [pid:40.5%,
                     q_cov:97.2%, s_cov:87.1%, Eval:4.1e-67]; Prodigal_141"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="CAL1238075.1"
                     /translation="MRMTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQIN
                     SKNEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDL
                     GFKFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLS
                     VVRLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYP
                     AFKRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNM
                     FDKMYPPEN"
                     /name="RepB family plasmid replication initiator protein"
     gene            886..1290
                     /locus_tag="PB31CTX2_1420"
     CDS             886..1290
                     /locus_tag="PB31CTX2_1420"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_142"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238076.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            1425..1952
                     /locus_tag="PB31CTX2_1430"
     CDS             1425..1952
                     /locus_tag="PB31CTX2_1430"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_143"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238077.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="hypothetical protein"
     gene            complement(2343..3089)
                     /locus_tag="PB31CTX2_1440"
     CDS             complement(2343..3089)
                     /locus_tag="PB31CTX2_1440"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_003112760.1"
                     /note="WP_003112760.1 hypothetical protein (Pseudomonas
                     aeruginosa PAO1) [pid:32.2%, q_cov:81.9%, s_cov:72.7%,
                     Eval:1.9e-21]; Domain:cl00474:PAP2_like N/A. This family is
                     closely related to the C-terminal a region of PAP2.
                     [Superfamily, Aligned:25-242, Eval:2.8e-19, score:81.1];
                     Prodigal_144"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238078.1"
                     /translation="MGGGVGFVMPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVA
                     DWFYLGMGHWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKW
                     LVPVTKFRVRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRN
                     TIPDKCFPAAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSH
                     TVVSMMLAWAMSAGLAWVFFKKGEQV"
                     /name="hypothetical protein"
     gene            complement(3137..4762)
                     /gene="mcr-1.1"
                     /locus_tag="PB31CTX2_1450"
     ARG             complement(3141..4762)
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5070..5573
                     /gene="tnpA_IS1X2"
                     /locus_tag="PB31CTX2_1460"
     CDS             5070..5573
                     /gene="tnpA_IS1X2"
                     /locus_tag="PB31CTX2_1460"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:ISFinder:IS1X2"
                     /note="ISFinderF:IS1X2 IS1 family transposase [pid:99.3%,
                     q_cov:89.2%, s_cov:64.2%, Eval:2.2e-108]; ISFinder:IS1X2
                     IS1 family transposase IS1X2 [pid:99.3%, q_cov:89.2%,
                     s_cov:64.2%, Eval:2.2e-108, partial hit]; frameshifted,
                     deletion at around 19948; COG:COG1662:InsB Transposase and
                     inactivated derivatives, IS1 family [Category:X,
                     Aligned:42-161, Eval:7.9e-46, score:143.7]; Prodigal_146"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="CAL1238080.1"
                     /translation="MPGNRPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDE
                     QWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWP
                     LYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNI
                     KHYQ"
                     /name="tnpA_IS1X2"
     gene            complement(5632..6336)
                     /locus_tag="PB31CTX2_1470"
     CDS             complement(5632..6336)
                     /locus_tag="PB31CTX2_1470"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001279526.1"
                     /note="WP_001279526.1 hypothetical protein (Vibrio cholerae
                     O1 biovar El Tor str. N16961) [pid:44.8%, q_cov:82.1%,
                     s_cov:68.1%, Eval:2.5e-56]; TIGR:TIGR03899; TIGR03899:
                     TIGR03899 family protein [Name:TIGR03899, Eval:1.9e-27,
                     score:94.4, bias:0.3]; Prodigal_147"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238081.1"
                     /translation="MSLIDLSLSGLSELGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLQIINTLKCIFSMGLRND
                     CLIKFLKYFCIA"
                     /name="hypothetical protein"
     gene            6340..6519
                     /locus_tag="PB31CTX2_1480"
     CDS             6340..6519
                     /locus_tag="PB31CTX2_1480"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_148"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238082.1"
                     /translation="MLLNTRATTVQGYFIARVTKNESQLHLKVMKLVHSDKYLKENDKE
                     FIGPLAMINHFCQL"
                     /name="hypothetical protein"
     gene            complement(6497..6766)
                     /locus_tag="PB31CTX2_1490"
     CDS             complement(6497..6766)
                     /locus_tag="PB31CTX2_1490"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_149"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238083.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            complement(6841..7488)
                     /locus_tag="PB31CTX2_1500"
     CDS             complement(6841..7488)
                     /locus_tag="PB31CTX2_1500"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_012599835.1"
                     /note="WP_012599835.1 peptidyl-arginine deiminase
                     (Escherichia coli UMN026) [pid:35.9%, q_cov:99.5%,
                     s_cov:97.7%, Eval:5.9e-34]; TIGR:TIGR03371; cellulose_yhjQ:
                     cellulose synthase operon protein YhjQ [Name:TIGR03371,
                     Eval:5.0e-09, score:34.0, bias:3.9]; COG:COG1192:BcsQ
                     Cellulose biosynthesis protein BcsQ [Category:N,
                     Aligned:1-202, Eval:2.2e-12, score:61.4]; Prodigal_150"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-arginine deiminase"
                     /protein_id="CAL1238084.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="peptidyl-arginine deiminase"
     gene            complement(7902..8081)
                     /locus_tag="PB31CTX2_1510"
     CDS             complement(7902..8081)
                     /locus_tag="PB31CTX2_1510"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_151"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238085.1"
                     /translation="MDFMSILGRCLLLPFPVAMFVGCLFPGIDDRLSGMLMSVAVGCLS
                     WYVTRPKNRQTKAA"
                     /name="hypothetical protein"
     gene            complement(9029..9733)
                     /gene="tnpA_IS26"
                     /locus_tag="PB31CTX2_1520"
     CDS             complement(9029..9733)
                     /gene="tnpA_IS26"
                     /locus_tag="PB31CTX2_1520"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:ISFinder:IS26"
                     /note="ISFinder:IS26 IS6 family transposase IS26
                     [pid:100.0%, q_cov:100.0%, s_cov:100.0%, Eval:8.3e-179];
                     COG:COG3316:Rve Transposase (or an inactivated derivative)
                     [Category:X, Aligned:1-214, Eval:2.2e-72, score:217.0];
                     Prodigal_152"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase IS26"
                     /protein_id="CAL1238086.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="tnpA_IS26"
     gene            complement(9820..10284)
                     /gene="hns"
                     /locus_tag="PB31CTX2_1530"
     CDS             complement(9820..10284)
                     /gene="hns"
                     /locus_tag="PB31CTX2_1530"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:SPE04298.1"
                     /note="resMGE:SPE04298.1 global nucleic acid-binding
                     transcriptional dual regulator (Escherichia coli IncX
                     plasmid) [pid:96.1%, q_cov:100.0%, s_cov:99.4%,
                     Eval:1.7e-96]; Domain:cl27638:Histone_HNS H-NS histone
                     family. global DNA-binding transcriptional dual regulator
                     H-NS; Provisional [Superfamily, Aligned:19-145,
                     Eval:3.4e-21, score:80.6]; Prodigal_153"
                     /codon_start=1
                     /transl_table=11
                     /product="global nucleic acid-binding transcriptional dual
                     regulator"
                     /protein_id="CAL1238087.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="hns"
     gene            complement(10302..10508)
                     /locus_tag="PB31CTX2_1540"
     CDS             complement(10302..10508)
                     /locus_tag="PB31CTX2_1540"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000850859.1"
                     /note="WP_000850859.1 transcriptional regulator
                     (Escherichia coli UMN026) [pid:100.0%, q_cov:100.0%,
                     s_cov:100.0%, Eval:2.9e-46]; Prodigal_154"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="CAL1238088.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="transcriptional regulator"
     gene            complement(10505..12658)
                     /locus_tag="PB31CTX2_1550"
     CDS             complement(10505..12658)
                     /locus_tag="PB31CTX2_1550"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:UniProtKB:B7LJB3"
                     /note="B7LJB3 DNA topoisomerase (Escherichia coli UMN026)
                     [pid:98.2%, q_cov:100.0%, s_cov:100.0%, Eval:0.0e+00];
                     TIGR:TIGR01056; topB: DNA topoisomerase III
                     [Name:TIGR01056, Eval:6.3e-161, score:535.2, bias:0.0];
                     COG:COG0550:TopA DNA topoisomerase IA [Category:L,
                     Aligned:1-622, Eval:4.6e-147, score:438.2]; Prodigal_155"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="CAL1238089.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="DNA topoisomerase"
     gene            complement(12663..13235)
                     /locus_tag="PB31CTX2_1560"
     CDS             complement(12663..13235)
                     /locus_tag="PB31CTX2_1560"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_156"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238090.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            complement(13546..13851)
                     /locus_tag="PB31CTX2_1570"
     CDS             complement(13546..13851)
                     /locus_tag="PB31CTX2_1570"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000820911.1"
                     /note="WP_000820911.1 IncN plasmid KikA protein
                     (Escherichia coli UMN026) [pid:81.7%, q_cov:92.1%,
                     s_cov:93.0%, Eval:1.1e-51]; Prodigal_157"
                     /codon_start=1
                     /transl_table=11
                     /product="IncN plasmid KikA protein"
                     /protein_id="CAL1238091.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="IncN plasmid KikA protein"
     gene            complement(13835..14254)
                     /locus_tag="PB31CTX2_1580"
     CDS             complement(13835..14254)
                     /locus_tag="PB31CTX2_1580"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000722607.1"
                     /note="WP_000722607.1 hypothetical protein (Escherichia
                     coli UMN026) [pid:34.5%, q_cov:84.9%, s_cov:86.9%,
                     Eval:7.0e-15]; Prodigal_158"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238092.1"
                     /translation="MKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFHG
                     VIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKMEG
                     ATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="hypothetical protein"
     gene            complement(14266..16128)
                     /locus_tag="PB31CTX2_1590"
     CDS             complement(14266..16128)
                     /locus_tag="PB31CTX2_1590"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_012599838.1"
                     /note="WP_012599838.1 conjugal transfer protein TraG
                     (Escherichia coli UMN026) [pid:40.0%, q_cov:93.9%,
                     s_cov:93.5%, Eval:3.8e-131]; TIGR:TIGR02767; TraG-Ti:
                     Ti-type conjugative transfer system protein TraG
                     [Name:TIGR02767, Eval:8.0e-26, score:88.9, bias:0.3];
                     COG:COG3505:VirD4 Type IV secretory pathway, VirD4
                     component, TraG/TraD family ATPase [Category:U,
                     Aligned:72-580, Eval:8.5e-77, score:253.1]; Prodigal_159"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="CAL1238093.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="conjugal transfer protein TraG"
     gene            complement(16115..17143)
                     /gene="pilx11"
                     /locus_tag="PB31CTX2_1600"
     CDS             complement(16115..17143)
                     /gene="pilx11"
                     /locus_tag="PB31CTX2_1600"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:SPE04309.1"
                     /note="resMGE:SPE04309.1 P-type conjugal transfer ATPase
                     Pilx11 (Escherichia coli IncX plasmid) [pid:98.8%,
                     q_cov:100.0%, s_cov:99.7%, Eval:0.0e+00]; TIGR:TIGR02788;
                     VirB11: P-type DNA transfer ATPase VirB11 [Name:TIGR02788,
                     Eval:1.9e-108, score:360.1, bias:0.0]; COG:COG0630:VirB11
                     Type IV secretory pathway ATPase VirB11/Archaellum
                     biosynthesis ATPase [Category:U, Aligned:22-323,
                     Eval:5.6e-74, score:228.4]; Prodigal_160"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugal transfer ATPase Pilx11"
                     /protein_id="CAL1238094.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="pilx11"
     gene            complement(17145..18263)
                     /gene="pilx10"
                     /locus_tag="PB31CTX2_1610"
     CDS             complement(17145..18263)
                     /gene="pilx10"
                     /locus_tag="PB31CTX2_1610"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:SPE04310.1"
                     /note="resMGE:SPE04310.1 conjugal transfer protein Pilx10
                     (Escherichia coli IncX plasmid) [pid:98.7%, q_cov:100.0%,
                     s_cov:99.7%, Eval:0.0e+00]; COG:COG2948:VirB10 Type IV
                     secretory pathway, VirB10 components [Category:U,
                     Aligned:190-372, Eval:6.8e-53, score:176.5, N-term
                     missing]; Prodigal_161"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein Pilx10"
                     /protein_id="CAL1238095.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="pilx10"
     gene            complement(18256..19164)
                     /locus_tag="PB31CTX2_1620"
     CDS             complement(18256..19164)
                     /locus_tag="PB31CTX2_1620"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000976329.1"
                     /note="WP_000976329.1 P-type conjugative transfer protein
                     VirB9 (Escherichia coli UMN026) [pid:39.7%, q_cov:98.7%,
                     s_cov:98.3%, Eval:1.7e-73]; TIGR:TIGR02781; VirB9: P-type
                     conjugative transfer protein VirB9 [Name:TIGR02781,
                     Eval:3.1e-66, score:221.5, bias:0.7];
                     Domain:cl11423:VirB9_CagX_TrbG VirB9/CagX/TrbG, a component
                     of the type IV secretion system. This family includes type
                     IV secretion system CagX conjugation protein. Other members
                     of this family are involved in conjugal transfer to plant
                     cells of T-DNA. [Superfamily, Aligned:1-297, Eval:2.5e-23,
                     score:93.6]; Prodigal_162"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="CAL1238096.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="P-type conjugative transfer protein VirB9"
     gene            complement(19161..>19262)
                     /locus_tag="PB31CTX2_1630"
     CDS             complement(19161..>19262)
                     /locus_tag="PB31CTX2_1630"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="WP_001005327.1 membrane protein (Escherichia coli
                     UMN026) [pid:58.1%, q_cov:93.9%, s_cov:13.7%, Eval:2.4e-07,
                     partial hit]; Prodigal_163"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238097.1"
                     /translation="FEPEKSVKSSSRELNPLGFTVTSYQTDREVRGE"
                     /name="hypothetical protein"
     gene            complement(<19263..19847)
                     /locus_tag="PB31CTX2_1190"
     CDS             complement(<19263..19847)
                     /locus_tag="PB31CTX2_1190"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001005327.1"
                     /note="WP_001005327.1 membrane protein (Escherichia coli
                     UMN026) [pid:39.2%, q_cov:96.9%, s_cov:83.3%,
                     Eval:3.1e-40]; Domain:cl01500:VirB8_like virulence protein
                     VirB8. This family includes the conjugal transfer protein
                     family TrbF, a family of proteins known to be involved in
                     conjugal transfer. The TrbF protein is thought to compose
                     part of the pilus required for transfer. This domain is
                     similar to the type IV secretion system (T4ASS) component
                     VirB8 and possibly has a similar fold to the nuclear
                     transport factor-2 (NTF-2)-like superfamily. [Superfamily,
                     Aligned:2-195, Eval:1.1e-28, score:104.4]; Prodigal_119"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein"
                     /protein_id="CAL1238098.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYR"
                     /name="membrane protein"
     gene            complement(20186..20365)
                     /locus_tag="PB31CTX2_1200"
     CDS             complement(20186..20365)
                     /locus_tag="PB31CTX2_1200"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_120"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238099.1"
                     /translation="MKIMKRFFYVMSLNWILVFVDKHITSGWSRKRKLITQLVILITGI
                     TLFKVLGGISQSWL"
                     /name="hypothetical protein"
     gene            complement(20362..21456)
                     /locus_tag="PB31CTX2_1210"
     CDS             complement(20362..21456)
                     /locus_tag="PB31CTX2_1210"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000513454.1"
                     /note="WP_000513454.1 pilus assembly protein (Escherichia
                     coli UMN026) [pid:37.1%, q_cov:97.5%, s_cov:90.2%,
                     Eval:4.3e-57]; Domain:cl01503:TrbL TrbL/VirB6 plasmid
                     conjugal transfer protein. conjugal transfer protein TrbL;
                     Provisional [Superfamily, Aligned:14-328, Eval:1.2e-09,
                     score:56.3]; Prodigal_121"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="CAL1238100.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="pilus assembly protein"
     gene            complement(21468..22184)
                     /locus_tag="PB31CTX2_1220"
     CDS             complement(21468..22184)
                     /locus_tag="PB31CTX2_1220"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000744204.1"
                     /note="WP_000744204.1 pilus assembly protein (Escherichia
                     coli UMN026) [pid:42.9%, q_cov:99.2%, s_cov:94.0%,
                     Eval:2.0e-62]; TIGR:TIGR02791; VirB5: P-type DNA transfer
                     protein VirB5 [Name:TIGR02791, Eval:6.4e-12, score:43.7,
                     bias:11.0]; Prodigal_122"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="CAL1238101.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="pilus assembly protein"
     gene            complement(22196..24943)
                     /gene="pilx3-4"
                     /locus_tag="PB31CTX2_1230"
     CDS             complement(22196..24943)
                     /gene="pilx3-4"
                     /locus_tag="PB31CTX2_1230"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:SPE04316.1"
                     /note="resMGE:SPE04316.1 conjugal transfer protein Pilx3-4
                     (ATPase) (Escherichia coli IncX plasmid) [pid:99.9%,
                     q_cov:100.0%, s_cov:99.9%, Eval:0.0e+00]; TIGR:TIGR00929;
                     VirB4_CagE: type IV secretion/conjugal transfer ATPase,
                     VirB4 family [Name:TIGR00929, Eval:3.5e-198, score:658.4,
                     bias:1.4]; COG:COG3451:VirB4 Type IV secretory pathway,
                     VirB4 component [Category:U, Aligned:114-913,
                     Eval:6.3e-168, score:506.5]; Domain:cl01501:VirB3 Type IV
                     secretory pathway, VirB3-like protein. type IV secretion
                     system protein VirB3; Provisional [Superfamily,
                     Aligned:3-86, Eval:1.5e-13, score:64.7]; Prodigal_123"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein Pilx3-4 (ATPase)"
                     /protein_id="CAL1238102.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="pilx3-4"
     gene            complement(24962..25288)
                     /locus_tag="PB31CTX2_1240"
     CDS             complement(24962..25288)
                     /locus_tag="PB31CTX2_1240"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000600171.1"
                     /note="WP_000600171.1 TriB protein (Escherichia coli
                     UMN026) [pid:44.6%, q_cov:63.9%, s_cov:76.3%,
                     Eval:7.1e-16]; Prodigal_124"
                     /codon_start=1
                     /transl_table=11
                     /product="TriB protein"
                     /protein_id="CAL1238103.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TriB protein"
     gene            complement(25302..25922)
                     /gene="pilx1"
                     /locus_tag="PB31CTX2_1250"
     CDS             complement(25302..25922)
                     /gene="pilx1"
                     /locus_tag="PB31CTX2_1250"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:SPE01517.1"
                     /note="resMGE:SPE01517.1 conjugal transfer protein Pilx1
                     (Soluble lytic murein transglycosylase) (Escherichia coli
                     IncX plasmid) [pid:99.5%, q_cov:100.0%, s_cov:99.5%,
                     Eval:1.3e-155]; Prodigal_125"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein Pilx1 (Soluble lytic
                     murein transglycosylase)"
                     /protein_id="CAL1238104.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="pilx1"
     gene            complement(25925..26101)
                     /locus_tag="PB31CTX2_1260"
     CDS             complement(25925..26101)
                     /locus_tag="PB31CTX2_1260"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_126"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238105.1"
                     /translation="MKEKSDKSSEKGVYVTIKLDPRLNAFIEEKTKTSLLSKRKQIIYL
                     LMQSMRIDGYMER"
                     /name="hypothetical protein"
     gene            complement(26278..26493)
                     /locus_tag="PB31CTX2_1270"
     CDS             complement(26278..26493)
                     /locus_tag="PB31CTX2_1270"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000835348.1"
                     /note="WP_000835348.1 hypothetical protein (Escherichia
                     coli UMN026) [pid:50.8%, q_cov:90.1%, s_cov:86.7%,
                     Eval:6.7e-14]; Prodigal_127"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238106.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="hypothetical protein"
     gene            complement(26490..26783)
                     /locus_tag="PB31CTX2_1280"
     CDS             complement(26490..26783)
                     /locus_tag="PB31CTX2_1280"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_128"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238107.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="hypothetical protein"
     gene            complement(26860..28089)
                     /gene="taxC"
                     /locus_tag="PB31CTX2_1290"
     CDS             complement(26860..28089)
                     /gene="taxC"
                     /locus_tag="PB31CTX2_1290"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_012599843.1"
                     /note="WP_012599843.1 spore coat protein CotH (Escherichia
                     coli UMN026) [pid:35.4%, q_cov:84.1%, s_cov:85.9%,
                     Eval:2.1e-62]; Prodigal_129"
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotH"
                     /protein_id="CAL1238108.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            complement(28093..28596)
                     /locus_tag="PB31CTX2_1300"
     CDS             complement(28093..28596)
                     /locus_tag="PB31CTX2_1300"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_130"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238109.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="hypothetical protein"
     gene            complement(28957..29304)
                     /locus_tag="PB31CTX2_1310"
     CDS             complement(28957..29304)
                     /locus_tag="PB31CTX2_1310"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_002668012.1"
                     /note="WP_002668012.1 antitoxin HicB (Treponema denticola
                     ATCC 35405) [pid:43.0%, q_cov:93.0%, s_cov:96.4%,
                     Eval:6.9e-30]; COG:COG4226:HicB Predicted nuclease of the
                     RNAse H fold, HicB family [Category:R, Aligned:1-108,
                     Eval:4.0e-32, score:106.3]; Prodigal_131"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="CAL1238110.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="antitoxin HicB"
     gene            complement(29301..29549)
                     /locus_tag="PB31CTX2_1320"
     CDS             complement(29301..29549)
                     /locus_tag="PB31CTX2_1320"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_132"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238111.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="hypothetical protein"
     gene            complement(29641..29778)
                     /locus_tag="PB31CTX2_1330"
     CDS             complement(29641..29778)
                     /locus_tag="PB31CTX2_1330"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_133"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238112.1"
                     /translation="MSTRQIVQLADSLVKGWETRIPAMKFSEWDQFRWWLNYLQGYEMF
                     "
                     /name="hypothetical protein"
     gene            complement(29854..30204)
                     /locus_tag="PB31CTX2_1340"
     CDS             complement(29854..30204)
                     /locus_tag="PB31CTX2_1340"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_134"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238113.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            complement(30295..30594)
                     /locus_tag="PB31CTX2_1350"
     CDS             complement(30295..30594)
                     /locus_tag="PB31CTX2_1350"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_012599848.1"
                     /note="WP_012599848.1 hypothetical protein (Escherichia
                     coli UMN026) [pid:99.0%, q_cov:99.0%, s_cov:86.7%,
                     Eval:1.8e-67]; Prodigal_135"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238114.1"
                     /translation="MKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVIFTDNNK
                     IRDYKAGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            complement(31048..31275)
                     /locus_tag="PB31CTX2_1360"
     CDS             complement(31048..31275)
                     /locus_tag="PB31CTX2_1360"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000801440.1"
                     /note="WP_000801440.1 hypothetical protein (Escherichia
                     coli UMN026) [pid:100.0%, q_cov:100.0%, s_cov:100.0%,
                     Eval:1.2e-50]; Prodigal_136"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238115.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            complement(31262..31513)
                     /locus_tag="PB31CTX2_1370"
     CDS             complement(31262..31513)
                     /locus_tag="PB31CTX2_1370"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001230707.1"
                     /note="WP_001230707.1 hypothetical protein (Escherichia
                     coli UMN026) [pid:100.0%, q_cov:100.0%, s_cov:100.0%,
                     Eval:8.4e-56]; Prodigal_137"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238116.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            complement(31510..32052)
                     /locus_tag="PB31CTX2_1380"
     CDS             complement(31510..32052)
                     /locus_tag="PB31CTX2_1380"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001083906.1"
                     /note="WP_001083906.1 hypothetical protein (Escherichia
                     coli UMN026) [pid:100.0%, q_cov:100.0%, s_cov:100.0%,
                     Eval:6.6e-134]; Prodigal_138"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238117.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            complement(32157..32672)
                     /locus_tag="PB31CTX2_1390"
     CDS             complement(32157..32672)
                     /locus_tag="PB31CTX2_1390"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_012599850.1"
                     /note="WP_012599850.1 molecular chaperone DnaJ (Escherichia
                     coli UMN026) [pid:100.0%, q_cov:100.0%, s_cov:58.6%,
                     Eval:3.9e-126]; TIGR:TIGR02349; DnaJ_bact: chaperone
                     protein DnaJ [Name:TIGR02349, Eval:4.6e-11, score:40.6,
                     bias:0.5]; Domain:cl28246:DnaJ DnaJ-class molecular
                     chaperone with C-terminal Zn finger domain
                     [Posttranslational modification, protein turnover,
                     chaperones]. This model describes TopJ (MG_200, CbpA), a
                     DnaJ homolog and probable assembly protein of the
                     Mycoplasma terminal organelle. The terminal organelle is
                     involved in both cytadherence and gliding motility.
                     [Cellular processes, Chemotaxis and motility] [Superfamily,
                     Aligned:9-54, Eval:3.8e-13, score:63.0, C-term missing];
                     Prodigal_139"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="CAL1238118.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="molecular chaperone DnaJ"
     gene            33507..33632
                     /locus_tag="PB31CTX2_1400"
     CDS             33507..33632
                     /locus_tag="PB31CTX2_1400"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_140"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1238119.1"
                     /translation="MIDDVCFSLRVIIIGGQIYGKEPSSLLVLLSYPQFLNIRTH"
                     /name="hypothetical protein"
     Typing          2..873
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(26864..28089)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(14270..16128)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(18260..19164)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19165..19847)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          complement(20390..21456)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.6999999999999997e-172"
ORIGIN
        1 atgagaatga cgacaaataa gacttccctt tctcgcttaa caaaagtgag acatcgaaat
       61 gaattgaatt caacgctgtc aacattgcct atggctgcta aaaaagtctt atttttagcc
      121 atgtgccaga taaactctaa aaatgaattc gatgatgatc acatatttta tgtgacagtt
      181 gccgattaca ttaaatgggt tcaggttaag cctgatgcgg cttatcttgc tttgagggat
      241 ggctctaata tattagatac gacgcttctt aagctgaaac atgatgaaat attagaactg
      301 agtagtgatt tgggatttaa gttcactaaa agtaatgtac ctgattcaat gaatttgagt
      361 ctgactgttt tttctactta ttacaggaat gaaggtagag tggggatcaa gtttacgaaa
      421 gaagcgaaaa gatttttatg taaacttatt ggtgaagaaa aacgttatac aacgcaagtt
      481 ttattatctg ttgttcgact gacaagtgtt aatgctgcat cactatatca actcattcgt
      541 aaaatatata gtaataattc tcgcgcgaat agttttgaaa tgacaataga tgaacttaaa
      601 gatgaattga atctttatac aataggggcg gggggggtga aggattataa gtatccagat
      661 tatcctgctt ttaaaagaga tgttcttaat aaatcagtaa aagaaatcat gaaacatacg
      721 gaagtaaaga acttaagttt cgtagtttca gaaaaaattg ggagaaaagt ttataaatta
      781 aagttctctt atacaattgg ttatgagggc gataccaggg aagactcaga gtttactaat
      841 atgtttgata aaatgtaccc acctgaaaat tgaggtcata acaatatgaa ggataatatc
      901 gtagattttc aaagaatcag ggacagcatg aatgatcatt caaccgatgt tgcaaatgat
      961 gccgtttatt ctgacaaaaa gaaaatatcc aggttctttt atctgctgac acctgtcaga
     1021 taccttctgg ctgcttccct ttatctggtt gttgttattc cattggggat tcttgcggga
     1081 tttcgtttct tgttgcagtt tttaggcggt atcgcagtga tgggtttaac tatcgcctgg
     1141 tacatgggtg cagatgtcag tagttacgtt gttcttgctg catggatcgc cttaactgtt
     1201 tgtggttgcg cagagcagat ttttgaatgg tggatcgaaa gccgttttgc gttccgtctt
     1261 tttggtatcg ggagaacgcc ggaagaatga gcgccgatct tacagacagt aaagaacaat
     1321 gcaggataga caatccgatg tcaaatgtta acatttgaat cagattgtcc aaaaacgatg
     1381 tttttgcctg tgcctgccgc aggctctttt aaaaggaata tgaaatgata tttaaccgta
     1441 ataaaaaacg agatgaacaa cataattctg ctgttgtttt gccatcagct gaaaatgtat
     1501 tctctgattt tattaagaac aatccagctt tgacaaccaa tgcttttaca gccattgggg
     1561 aggttgattg ttttactaaa gtacccttac tctcaaataa tgaatcagag tttttaggca
     1621 ttctttcacg taatattata cctgattatt ccgtttttcc caaaatacgg ctcattgaat
     1681 ttgttcgtcc tcatggtagc gaggaagctg taaaagattt aatccgtgaa gtgcagaata
     1741 taacctgtga ttttgtcctg aaatcatatg atgaaacagt cctggctgtt atccagtttg
     1801 gtgaaaccgc aaccgtcaga cagcaaaaga aaaaactgat tatccagcgc gtgtgtgcta
     1861 tgacaggaat ttcttttttc gaatttaaaa acactgtcga tatgatgggg agcgaagatt
     1921 tttgtcagtt cctgcgggat gatgaagagt aattaaaaat aagcccacat atttgcgggc
     1981 ttaacaggct ctttattagt acttgctgca tttgtgatta ctggcaacac agttttcact
     2041 gggcagtttt tgcggtcgct tttaattatg gttagtggaa ctcagacagg taactcgacc
     2101 tctttacata ttaataattg atagttttac ctggcttcct tcaacattta ttttgttgag
     2161 gaatttattt aaatacgcaa tatctggagt ggaaccgcat attttcatca attcttgacc
     2221 gataggcgta agtaaagccg ttcctattgg tagtttccag gttgatgaat catcgctttg
     2281 aaactcaact gaaaaaaccg ttccgtaata accgatacga ccgtttttac ttttaaaggc
     2341 gattaaactt gttcaccctt cttaaaaaac acccacgcaa gccctgccga catcgcccac
     2401 gcaagcatca tcgacaccac cgtatggctc aaaaaatgat cgccaataat catcttatag
     2461 cagcccatcg cccagcccaa tgccatcacc acgatgatga tcgcaccacg acggcggcgc
     2521 aaacttggcg caaacgcaaa ggcaaacagc gcaaatccgc tactggcatg cgccgcagga
     2581 aagcacttat caggaatggt gttacgcata ctatcaagca tcggcaaata cggcagcgta
     2641 ccatcaaaaa tcgtcaaatg cacagggcag accacatgag tatatgcctt caatgacgcg
     2701 acaactgtcg gcactaggat caaagtcagc accagatacg caagctcacg cacgcgaaat
     2761 tttgtcactg ggacaagcca cttggcagtg atagcattgc cctttttctc atgataagca
     2821 cggcaaatgg tcgcaaccag caagtagatc agcatcgcca tcagcagctt ttttagtcca
     2881 gaatacagta gcaaatctgg caaaaaagct tgcttggcaa ccatccaatg ccccataccc
     2941 agataaaacc aatcagcgac catcagatca atctgactgt gctcaagggt cagcgtggca
     3001 acgatcgcca tgacaagagc gatactcatc tcagcaagta ggcgtttatt tgataaatac
     3061 ggcataacaa accccacccc accgcccata atacgaatgg agtgtgcggt gggtttggaa
     3121 aaaatacagg gagaaatcag cggatgaatg cggtgcggtc tttgactttg tccgcggtga
     3181 catcaaacag ctttaataat gtcggcgtga tcgcgtcatg ggtcaggacg gtatcggttg
     3241 ccattggcgt gatgccagtt tgcttatccg tccagaaaaa tgcaggcaca ctgcgctgtt
     3301 cttttggtgc aaaggcattt ggcataccat gtagatagac accgttctca cccagacttt
     3361 cgccatgatc gctgacatac agcattgaga catcataggc attgctgtgc gtctgcagcc
     3421 actggatact ttgagcgatg aaatcatcgg tggcaagcaa ggcattgtca taagcattga
     3481 tcaaggactg atgttcgcac ttggcaagct cattaccttc acacactggc gtgaatttgg
     3541 caaacttttc atcatatcgc ttaaaatacg caggcccgtg attgcccatt tggtgcagca
     3601 tgatcagcat atctttgccg ttattggcag cgacaaagtc atctaagcca acgagcatac
     3661 cgacatcgcg gcattcgtta taaggattgg tgttgcagat ggcgttgttg gtcgcggatt
     3721 tataatcggc aaattgcgct tttggcagct tatccatcac gccttttgag tccgaattat
     3781 tatcacgcca caagatactt acgcccaagc gatccagcgt atccagcaca ttttcttggt
     3841 atttggcggt atcgacatca tactcatccg cgcccagata gctgaacata cacggcacag
     3901 aatacgccgt cgatgtgccg cacgatgtga cattgctaaa attggtcacg ccatcgatct
     3961 tggcaagctg tgggaaagta tcgcgctcat agccattgaa gctgacatga tcggcgcgtg
     4021 ccgtctcacc gacgacgaac accactaggc gtggcttacg catatcaggc ttggttgctt
     4081 gtaccgcgtc tttggcgtga taaatggtat cttttggcgc actggctttt ttatactcaa
     4141 tactggcaag cttacccacc gagtagattg gcatgatcgg attgacatag ctacgcagcg
     4201 gcttatgcac gcgaaagaaa ctggcataat gactgctgaa cgccaccaca ggcagtaaaa
     4261 tcagcgcaag acttgccacg atcaagccca atcggcgcat caaacccttg ccccaagtcg
     4321 gataatccac cttaacaaaa gccacaagca aacttggtag cacacccaaa ccaatgatac
     4381 gcatgataaa cgctgcgttt aatagatcct tggtctcggc ttggtcggtc tgtagggcat
     4441 tttggagcat ggtcgtatca tagaccgtgc cataagtgtc agtaaaataa ctggtcaccg
     4501 cgcccatgat taatagcaaa atcaacacag gctttagcac atagcgatac gatgataaca
     4561 gcgtggtgat cagtagcatc gcgccaaaga gcacgacagc gatcgtcagc acaaagccga
     4621 gattgtccgc gatgggatag gtttggctga ttttatcaaa aaaggtaaga ttggcggtcg
     4681 cggtcaagaa aacggcaaca ctcgccacaa gaacaaacgg actgaccgag cgtcggtacc
     4741 acacagaagt atgctgcatc atgagaaact actcaaaaaa taaacggtgg gataattgcg
     4801 gcaattatac tgcaattttt ggtaatggtg ccaacttact gatttagtgt atgatggtgt
     4861 ttttgaggtg ctcccgtggc ttccatctcc atcagttgtc cctcctgctc agctactgaa
     4921 ggcgtggtgc gtaacggtaa aagtactgcc ggacatcagc gctatctctg ctctcactgc
     4981 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
     5041 atcattgata tggccatgaa tggcgttgga tgccgggcaa ccgcccgcat tatgggcgtt
     5101 ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgca
     5161 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggctacgtcg
     5221 gtgctaaatc acgtcagcgc tggctgtttt acgcgtatga caggatacgg aggacggttg
     5281 tggcgcacgt cttcggtgaa cgcactctgg ccacactgga gcgtcttctg agcctgctgt
     5341 cggcctttga ggtcgtggta tggatgacgg atggctggcc gctgtatgaa tcccgcctga
     5401 agggaaagct gcacgtaatc agcaagcgtt acactcagcg cattgagcga cataacctga
     5461 atctgagaca acatctggca aggctgggac ggaagtcact gtcgttctca aaatcggtgg
     5521 agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcag taagttggag
     5581 tcattaccca attttttaag aaatccagta caatttatct gatataaaaa attatgcgat
     5641 acagaaatat ttcagaaatt ttattaggca atcattgcgc aatcccatac tgaatataca
     5701 cttgagagta tttataattt gtaaactata accaatgcct tgttgaataa cgcctaagga
     5761 gtctaaaacc gaaagtttat cgaaagttat accttcagta tctccattct catcggtaaa
     5821 taaaattgga gtgagtttgt ttgcctgcca aacaaacttt ccaaaataag taataaggtt
     5881 cgcttcttct ttgcttatag tggataatag ttttaaggtt ctacgactaa acgaaccttt
     5941 tgactttgct tcttcggata aaattcttcc ccaaagcatt tgcatttgtt cgtcactaat
     6001 atcctcacac tccctgaaga aagcctctat ccaatcttcg tcaatatcag aaacattatc
     6061 actctcagat agactttgtg ctgcttgcat tgttatattt tcaatgtttt cctgtcgttt
     6121 cgtttcacgt ttgagaaaac gctcaactgc ccgtttttct attccctcaa gctctaacct
     6181 agaaattagc tctgtgcgtt tagcttcagc ttcagccttc gcttttttcc tgattcttgt
     6241 aggttcataa agcacaccaa tagcatcact tattttttca ataagttttg ttcctagttc
     6301 tgataatcct gataaactta aatcgataag gctcatctag tgctcctaaa tacccgtgca
     6361 acaactgtac aagggtattt tattgcaaga gtaactaaaa atgaatcaca actacattta
     6421 aaagtgatga agttagtaca ttcagataag tatctaaaag aaaatgataa agagtttatt
     6481 gggccacttg ctatgattaa tcatttctgt cagctctgaa ccacaaatta atgagttcgt
     6541 tttcgtccag atcctgataa gccagtaatg ctgctttgat tatttttgtc ttgggacgat
     6601 gcagtctttt tgattcgtta tcaatgaatt caagcaattc attcggtatg cggaatgaag
     6661 ccagttttgt tttgttctct gtggggcgtt ttcctgcgtc ctgaacaaat tttgtggcgt
     6721 tcacttcctg gttttgttct agttctggta gttccagctt tgccatgatg taccttctaa
     6781 tctgattgtt tcacactaat gtatatcatg atgatgtaca ttagtgtatg tcactatgat
     6841 ttacagttcc agttctttca gcaagagttc gatttgtgct tttgcctggc tggcaccgct
     6901 aacattactt tttaactcgt gaacccccat tccctgattc attgctctta caaaggcccg
     6961 gagaaaagaa accgtgcttt tcattggttt aagccagtaa tcatggctgt tgagagagtc
     7021 tcttaattct tgttgttcac tgaacgtatt tggctggcat ttatacagga gcacatgtgc
     7081 agccagcctg ggatttattt tttgcgcatc cctgacaatt tttgtcacag tctgaagact
     7141 gtctgcttcc acctgggcaa gcgggtcgat cgggacaatc acaatgtctg caacttttag
     7201 tgcagttctg aactccgtgc tgtcatatcc ggcagtatct ataatgagga tttctgctac
     7261 atctgccatt tttgttattt tggcactgat gtcgccatag gcttcattta atgcgacggg
     7321 cggtaatccg gcctgttctc tgcgtttatt ccattttttg gtcgagccat tcaggtctgt
     7381 ttcaacaagc cccgttctct ttccctgttg tgagaggcaa acagttaagt tggttgccag
     7441 atgtgattta cctgtaccgc ctttatcacc accgattacg ataatcatag taccccttgt
     7501 atgtcacagt gatttactca tgtgtatttc tatgtgatgt acgcatgcgt atgtcacatg
     7561 gacatactat agtgtatgtc acaatgaatt acaatgatgt acatcatgaa gaaatacatt
     7621 attgtatgtc agtgtgatgt acccgttggt tggtcacttg aaagcaaaag tgacagcgtt
     7681 actttcggta tcctcccttg ctgcgaaacc ttgtggcaat acagcaatga gtatgtttag
     7741 ttacaacatt caaaattaag cgattagatt ccgcccgtac gggcggggcg cgccactttc
     7801 tgtagtgggg aggggcaggg aggttatgtt tgctggatga gcggaggatg gaatgagcta
     7861 ttagaatgtt gtaactaaag ggtatcccaa ttaaaggcca cttacgcggc ctttgtctgt
     7921 cggttcttcg gtctggtgac ataccacgat aaacatccca cagcaacact cataagcatt
     7981 ccggataagc gatcatcgat gccaggaaac aggcaaccga caaacatcgc aacagggaaa
     8041 ggaagaagta aacagcgccc caaaatgctc ataaaatcca taaatcctcc ttttttgtta
     8101 aagggcagga agcggttcac ttcctgccct tagttacgtt tccgttgtca tcaatcgatg
     8161 aattggcgga tcagttccag aactgcaatc agcgcgtttg caacggcgac cacagctctg
     8221 agaacgatga ttaacatatt aatcatccgt ttctccagta aaactggagg aaatcaccgt
     8281 tgcttgctca tacactgcct gtcacaacgg ctctctcgca ttgttaggtg tagagtgacc
     8341 tcctcctgcc agagcaccga atacagcacc acctgtttat ccagccagga ctttaaaatc
     8401 gttcataccc gcagatacgg gaacagcagg attgcaagcc aactacatga ctaactgact
     8461 gattatacgc tacacaaaac acccgttaca ccactaaatg tggttcctta accacattta
     8521 gttgctttac agcaccatag tgaataaaat gtaaacgtta ggtgtagagt aaccttctcc
     8581 ggtcagagcc accaactcaa gactggaaaa cgcaaaaaag cccgattacc agtcgggttt
     8641 ttttgtgcct gtcgttcggc tgtagttccg ttttttctcc ttcatttgag ggatttttct
     8701 ttcatttttt ttgttgcttc caatactgcc tgtcgcgttg gccaacagct tgacgcgtta
     8761 ccggcatttt cattactcaa tatttttcag tgaattaaaa tgcaacagcg acagaaaagg
     8821 acattcagct ttttagatga atgctatagc aacggtgtgc tacgtaaaat ctgcgaaaaa
     8881 agcaggggta gggtaggtta tttttcacgt agcaacgcca tctggcactg tacataggta
     8941 tgtcacacag acatacctat gtgtatgtct gtgtgaggca ctgttgcaaa gttagcgatg
     9001 aggcagcctt ttgtcttatt caaaggcctt acatttcaaa aactctgctt accaggcgca
     9061 tttcgcccag gggatcacca taataaaatg ctgaggcctg gcctttgcgt agtgcacgca
     9121 tcacctcaat acctttgatg gtggcgtaag ccgtcttcat ggatttaaat cccagcgtgg
     9181 cgccgattat ccgtttcagt ttgccatgat cgcattcaat cacgttgttc cggtacttaa
     9241 tctgtcggtg ttcaacgtca gacgggcacc ggccttcgcg tttgagcaga gcaagcgcgc
     9301 gaccataggc gggcgcttta tccgtgttga tgaatcgcgg gatctgccac ttcttcacgt
     9361 tgttgaggat tttacccaga aaccggtatg cagctttgct gttacgacgg gaggagagat
     9421 aaaaatcgac agtgcggccc cggctgtcga cggcccggta cagatacgcc cagcggccat
     9481 tgaccttcac gtaggtttca tccatgtgcc acgggcaaag atcggaaggg ttacgccagt
     9541 accagcgcag ccgtttttcc atttcaggcg cataacgctg aacccagcgg taaatcgtgg
     9601 agtgatcgac attcactccg cgttcagcca gcatctcctg cagctcacgg taactgatgc
     9661 cgtatttgca gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt
     9721 tgaatgggtt catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac
     9781 tatttgcaac agtgccctgt gtgatgtata cttggcgggt tattctgcct gttccggctt
     9841 agttttctca atgaggaaat cttccatgaa atgcccctca tcaagcagct tctgtaaagc
     9901 aagcggacgc ttgccattac cagaccattc tctgatctcg ccattttcat ccttaaagcg
     9961 gtatttagcc tttcttactc cgcctttggc tttccgcctg gtttttactg tatctgctgt
    10021 tacaggagca aggagtgatt cgggatcaag acctaaagat atgatgtatt caatggcctg
    10081 ctgacgtttg gcttcaagtt cctggcgttc agccgcttca cgttcagcat cttcacgacg
    10141 ttcttcaata acaacgttga gcttttcctg catttcgagc aactgctcaa aatcaatctc
    10201 acgggcaaaa acacgcaatg aacgaatatt catcatagta cggctgataa tgccgtattc
    10261 atcttcttta gtcagttcgc tcatataatt aatcctctta tttagtgtac gtacttccag
    10321 acgccggaag gcaccttatc ataaagttta ttcatcacca gttctgctcg gcgatgatct
    10381 gcggcagaat aaaatgcctc tgattctgat aaaggcagtt tgtaacggtt aatctcaata
    10441 actttttcaa gagtatctct tgatgtacat tttcttaact gaaaaagcca ttcctgcttt
    10501 gttttcactt cttagccctt gccggaaaac ccagcttccc tgttttctta tcctcaagga
    10561 caagaactgt atcatacgtt ccaccacttt tctttttaaa tcccttaatc aaatcggttt
    10621 tccctgattt aaccagtttt tcggcctgtg cctgggtgat tttcttacca gaaaactcac
    10681 tccagatttt aaattcacat ccggtacaga aataaccttt cggcctgata acaatatgtt
    10741 taccacagga agggcaggga gagtccaggc gttgaaactg ccctgctggc gcagcgggtt
    10801 caatcttcat ctcgcccagg tcaacatcag aaatcatgcc tgtcagttca ccgtacagat
    10861 cattaataaa ctgttcaacc gtcaggtcgc cattttctat ggcagcctgc ttttcagacc
    10921 ataatgctgt catatcagga ttaaccgcta taccaggcag ggcatcaata agcgcatatc
    10981 cggtatcagt aggaataagt ttcccttttt ccagcgtgat atagtttctt ttcttcagcg
    11041 tttccagaat ggctgcgcgg gtagctggcg taccaatgcc accatgttca tcttttttgt
    11101 ctttatcctt atccttcaac aattttttaa ttgttggatc agtgacaaaa tccgcgacac
    11161 gaacaagcgc agcaagcaag gaggcttcgg tgaataacgg cggcggtgtt gttttcttct
    11221 cattaacaat aacttctttt gtcgtcagtg tttctcctgt gcgaatttta cagagcagtt
    11281 caaaagcgga atcatcatta tcttctgatt caccttcgtc tgtggtttcc gcgccaagaa
    11341 acgcctcaaa tccgctgtca gttgtttttc tggcacgggc atagaacgac tcatcaccac
    11401 actgaatggc aaccgatact tcctggtatg ctttttcagg catgaactga acaagatagt
    11461 gttgtgcgat cgccaggtaa acattgcgct cgtcggtgct gagtgcgtta acatcaggca
    11521 cactggcggt cgggattatc gcagtatgcg cagtcacctt tgcactgtta aacgctttgc
    11581 ttttacgtgc agaatcaata tccagcgact gaggaaagac tgatttcagg gcatcgataa
    11641 cctgcggcgc ttcgctgaat tgttcatctg aaagatatga gcagtcagag cggttatacg
    11701 taattgcttt atatttttcg cgtagttgtt gcgtaatatc cagcgttttt tgtgccgtca
    11761 ttttaaactt cttgttcatg tattgctgga gtctgaccag attaaacggc aacggcgcag
    11821 ccgttttttt atcatcagtt gctgctgctt caactgtagc cggttttcct gcaagggatg
    11881 cagccgttcc gtctgcccac gctttatcaa gtaatttacg gtctgtcagc ggagcaaatt
    11941 cccctggctt ccagttcgcc ctgataacat cagcaccacg ctgaaagact ccggtcatgg
    12001 tgtagtaaaa actggattta tggttctggt tagcacgggt acgattcaca atcaggccaa
    12061 gaacgggagt ctggacgcgc ccgacagaca gaacgccctg atatcctctg gcttttgccg
    12121 gaatggtgta cgcacgtgtc atagagaagc catagacggc atcggcaact gaccgcgcca
    12181 acgccttaag gtaaagtcct ttgaaatcac gattgtcttt aagatttgcc agtgcctttt
    12241 tcactgccgg aagcgtgttg tcgttaatca gaacgcgctt tacgggtttt gtgttgcctg
    12301 catattccag gacttcatca accagtagct gtccctcatc atcaggatcg ccagcgtgaa
    12361 caatttcagt cacgtctcca cgtctgataa gttcgagaat cgttttaacc tgttttgctg
    12421 cactttcaac aggctgatac ttcacgggat aaagacgtaa aggaagcgtt tcaaccttcc
    12481 aggctttgta ttcaggatta tagttttccg gcggttgtga ttcgataata tgaccaaaac
    12541 agttagtcac aatggcgtta tcactttcaa accagccatc atggcgggta aaattgccac
    12601 caagtgcctt aacaatatca tttgctactg cgggtttttc tgcgataaaa agtctcattt
    12661 tgttatcctt tacattgaat taatcttttg gcacaacgga tagcgcgttc ttccgatact
    12721 gtatgttgcc agttatcttt acttactact ctgaatctgc gctcacagcc attaaaggca
    12781 aaaattccct cacggttaag tatattagtt gcaatatccc actctttaat tctcaaaaag
    12841 gctgttacag caaaaccaga atacgaagca atttttgaaa tgatgcattc atcagctttt
    12901 attaacgcgg caaaaggaac tatcttttga tgaccacacg aaatcagagg gagtgtgtga
    12961 atatcataat aagctattga atgacaatcc atgcgggtat catcaatagc tgccagccag
    13021 aatcctgcct ggtcttttat ttttctgcta atatctgaat ctgaaacacc agggtttaat
    13081 aaatatccac cataacagat atggaagggg tgagcagatt catcaatatc aggaagttct
    13141 tttaccggat tttctttcca gtccttcaga acaccgaatt caagagcatc cagcaaaaac
    13201 tttcgttcaa atccatcaaa caatgcatat tgcatgtgtt aatcctccag gtaacttaaa
    13261 tagtcaggtt gtgccccgtt ctttttataa agttcttccc tttcctctat atcttcatct
    13321 gcttcctgaa cgctcagatc gagggttcct ttcaaaaagg cacgagcatt ttttattaca
    13381 tccacttatg cactaccagc agataagcac aataaaatag ctataataga tttggttact
    13441 ttcatttcta acctcagtga ttgtaactaa tcatcacatt tcgttggcgc gaaatgtgat
    13501 acttgcagta ccctgatacc cgttggcgcg gttatcaggt tttaattaac ctctgacacg
    13561 accaaactta ctcatgattt ttgatatagc agcaggatca gccgttgaac actgattcag
    13621 aaatgcttta cgggcatcaa aagttttact ccagcgaatg cttccttttt tctttttcag
    13681 aatattaaaa aaatcctttt cagcacttct gcattcactc cctccacctt gcccgactgc
    13741 ttttccatat aagcaaagta ccgaagcgca agggtcagat gcattagcat ttgttgatat
    13801 taatcctaac gccagcaagg ctggtaaaac cactctattt tttccagtca tttttcacct
    13861 cattttgtgc tttaatgaaa actattttta cctgttcagg taaaaaactt ttctcgtttc
    13921 gccattccac gactgctgtt gccccttcca tcttcaacag atctctgatt cgaaaccaca
    13981 gcccagcatc atgtgttgtc actacaatcc tgtccgcatg agccagagcg taataaaacg
    14041 tcggcgttct ggcctgtact ccggataagg aagtgatctc ataaacccat cctttaccgc
    14101 taacgtctga ctttatgaca ccgtgaaaat cggggacata tggcaatgag ggattgatca
    14161 cttcatttgc cttttcaaac tgcacgggtg ttggctctgg cggtgatgaa caaccagcaa
    14221 gaatggaaat caataaaaac actaacttct tcatcatgtt ctcctttatg ccgctttatc
    14281 gagatcagga acatctttta ctaccagata cccttttgcg acgatttcat tttcaaatac
    14341 tttctgtgga ggcataacca gctttccttt cttcctgggg aaaatgtttg caagttccgg
    14401 agcaaccttc acaaagtcac caaagaaata ctcattgaga tagtagaatg ccttttcaca
    14461 acgaatcggg ttttctccct tcattgccag gatttcttca ctaaatttca gtgtaagtag
    14521 ttcatttgtt gttaccagtg gacgctcaat aaggctttct gattcagatc tcgatgttcc
    14581 tccacgacta cgacttttgc ttttcgaacc gatagttact gttcgtgtcc ccatcgcttc
    14641 tgataactcc cgcgcatctt taacatcaga agcggaataa acaattttgc agggtgcact
    14701 tgccagtaat gttttactgc ctgcataact gtagatttcc tcaagctgag agccgacctg
    14761 gtaaacaatc aacaatttca gcttgtaacc agcaatgaga ccagatttac tcttgatgta
    14821 atctacagca ccaattgacg ggaattcatc cagtagaaaa aggcagtcat gctttatttc
    14881 cggatcatcc gttggcatct tttcaagatt cacctgtata gcaagctgaa agaaaatatt
    14941 tgttattttt tcagccaacc tggcattatc accagaaata cagaaataaa ttgtcatccg
    15001 ctttttccgc acctgccgca aatcaaaatc atttccatca gtagcatttg ccacattggg
    15061 gttagcaaac ggtgataatc cattaagaaa gttgcttttg agttgtgctt ttgttttatc
    15121 ctccatctca atgaatgcct taaggccagc gacaccaata tcagcaagcg ttttgtcttt
    15181 cccccttaat tctgaacgaa tatcattcat gaatgaaaga agatgttcgg gttctgcacg
    15241 ctcaaaaagc tgattaattt tagacattga gaagacaggc tcaatatcaa gcgtttttag
    15301 tccagcctta tcattcttaa tcataaacca taaagcgaca gcataagatt taaaaacacc
    15361 acccgccgtc ttattaagat agctaccagc atccgcacca tctgtcttat aggggtaaat
    15421 aatcaacgaa agattttcaa gctgattaaa accttcactc gttcccatgc tgacataata
    15481 caaagggtta aagcggtgcg ttaccgaact aaagggatca aatttataaa cctcatgacc
    15541 taaaatcacc ttcctgataa gactggtgat attaaaacac tccctttttg gatcgagaat
    15601 aataacactc caccagtact tcataagatt agtgatgact ataccagcac ctttaccagc
    15661 ccttgttccg gctcccatag cactgaatgc tgctcccaca tagcgtatga ttttcttgtc
    15721 ttttatacca acgataatcc cgtctttttc gtcaccgtca agcgtgaaac tgttagactc
    15781 acgaatatca ttaattgttg caaatcgcgc atcaccgaaa atattttctt tcttctcgtt
    15841 cagttttatc aacgccgcaa cagggatgat aagagacacg acaaatcctg ctccaagtgc
    15901 aacaaaggct aaaactctga tatccgtagt cacaacagat aaagtaaggc tgtctgtaaa
    15961 aatatccatc ctgtacgcat caatggatct gaacacagcc ttcaatggtt tcagattctt
    16021 tatctggctg aagtaaagca caaagaaaat aaatgaacct gcaaaccatg ccgcaacaag
    16081 cataacagcg aatataaaaa cagattgcga cttattaaaa gttttcacat tcagcccctt
    16141 tgtaatagat gtcgctcata tgcctgacaa caccatccct gcctacatga acaacaatat
    16201 caatactgtc cataatgatt ttacgaagaa catcaaaagg aagattctga cattctctgt
    16261 tctgataaca ttttgtcacc atctgaatga tggcatcttt tgccgaggcc gcgtgaatgg
    16321 acgtcatgct tccactgtga ccagaaccag caactttcac aaaatcccag gcatcgccac
    16381 ctttaatttc cgccagtaaa attctgtcag gtttcatcct caaacaggat ttgattaatt
    16441 tcgctgctgt tatgatagag ccggaatcac tgccggattc cgaagggtaa aaaagatgca
    16501 cataattttt atggataaaa aatttgattt cctctgcatc ttcaatcgta atcagccgtg
    16561 tattcaatgg tatgaaatcg ataagactct tcatataagt tgttttaccg gaacccgttg
    16621 ctccggcaaa tacaatggtt ttccctgctg ctacggcaag ctccataaaa gcagcgatat
    16681 ttctcgctct gaataaatca agaagctcat catcgtatgt cctgtgtttt tttcctgact
    16741 ggacataatc ataaaaaccg ttcgccacat attctttatg tgttatctgc cgtgtcgatg
    16801 gtttacgtat agtgattgaa atagtatttc tctcacatgc aggaggaaaa acgacctgta
    16861 cacgttctcc tgattccagt gttgctgaaa gtaatggctt ggtctcgccg atttcatctc
    16921 cccgaaatga tgccagcgta tgagaaaaac gtttacaaaa atcaaaggtc acactgtcat
    16981 tattatggaa tgtccagtca ccattaattt ccgtccagat ttcaccagga cggttgacag
    17041 caatttctgt tagcccttca atatcaagaa aagactggaa tacttgattt ttataaaaat
    17101 cgagcgattt attgctaatg ctgacaggaa taacgccact catactattt tacctttaac
    17161 tcatagatat cagaaaaatc aatgtcttcc ccgaccataa tagtgattat gtcaccctgg
    17221 tttttataca ttgttggagg aatatttatc gaattctcaa gtgcgacttt agccatttca
    17281 gccattgcct gtcgtgagtt ttcggtataa tccacattac gatctttacc aggtgcatta
    17341 tccgccgccg ccgccgctac atcctgaacc atactgagca taagagaacc accaaaacgc
    17401 tcccagaagt gagaatcgat ccatccatca atgcccgctt ctcccagggg accagctgct
    17461 gctgtattta ccatatcgat tcgtttatta tcaggcgtcc gcaactgctc ccacataacg
    17521 aacattctgc cctgtccatg attcagcgtt ccggtctggt aacgaccaaa agccttagtt
    17581 ccctgttcca gtaattttgt gtggtgatta gcactccata catcttctgt aaaaacacaa
    17641 ctgatacgac ctgcaacatc agaaacaaag cgtgtggtta aagcacacgg tataagcctg
    17701 ttttcctcaa tatagagatc aggatcgaga ttaatcgcag ttactttcat tcctgattta
    17761 ccaggcacat tttcttttga ttctttcctt tcttcctgtg gctctgatgt ctgcgatgtc
    17821 ctgctgctgc cactcgcgcc agtctgactc cctccctgac cagcaacagg tattgacaaa
    17881 tatttcctga acccctcctg tggcacttct ttatgaccgt cagaaacagt attaccagaa
    17941 ccagtagcgc ctggattgat aattgctttg ttctcaacag ggtcaaaatt ctgaccaaga
    18001 ccagtacgga ttttacttac ctgagaaatg ctggtatcgc ctgttgattc tgatgctttc
    18061 tgctggtcat ctgcaaaata atttttatat gttttcagcg caaaaatacc aataactaat
    18121 acagccatca gaccaaacag aaagccagaa cgcatacgat tatttttgtt cctgatatca
    18181 ccgatatcat gaacgggttt actttccagc tttgactgat tcgcatcatc aactaaatca
    18241 tctgtttttt tcatatcagt cattattcac ctcaacacgt tctaccgcag gagaagatgt
    18301 attcctctgg tcggttaata cttttccgaa agatttattc acaacaccaa caacgctgtt
    18361 tccatagcgt aaaacaaatt tgtcattaac gttgtggata accataattt tatacttgcc
    18421 ttttgtttcc acattaaaag caagtgtctg ttcctgacca tctcttacag caaatggagc
    18481 tgggaatact ttccctgagt taaaacctaa aaaggtaaat atgccattat cataagcaaa
    18541 atcaggggcg atacgacgac tgtcatattt ttcattgact cgcatgaaat aattccagtt
    18601 tttaggcgca tcggcttttt caaaactttc agaaatcagt ttcttctgtc tgagtttttc
    18661 ctgtttttct tttgccagcc ttacttcttc tgcattcttt ttcgctattt ctgatggata
    18721 gcggaattgt acaacataag caggttgcgc ttgtttttct tctgacagaa ggattagatc
    18781 cagactgtaa atccgctttg ttgttctgac aaaaagattc gtatcccatt ccttttctgt
    18841 tggctggaaa acctttttca gtttttcgcc ttcatcgcct tcctgttcct gaacaacggg
    18901 acgcggattg atgtaaacaa cattatcatc ggttgtgatt tcccatccgg caggaaaacc
    18961 agcctttgca ctgataacag cctcaccttc atcaaaaaca actgatgtga gaaatcctgt
    19021 ctttgaccgg ataacggtcg tattcgcacc gttataactc acagtctgca tccttgcatc
    19081 aaatccagaa ggtaacggag tcgctgctga atacacaagc ccaggaagca ataaacatgt
    19141 agcaataaat aatttcttca tcattccccc ctgacttccc gatctgtctg gtaactggtt
    19201 acagtaaaac cgagaggatt aagctcacga ctggaacttt taaccgactt ttctggttca
    19261 aaacgatatg tcacacgagc cgtccagtat gaaactgaaa cctgtccggt cgtgaagttt
    19321 ctcgtcgttt ttttaaagcg cacagaggcc agtttatctg gcgcggttgc gtcagaaata
    19381 atattggaga tgacctcaac cgcaacgtaa gaaccgttat tatgataaat tttatcagga
    19441 gccatatcgc tgttatacag acgaagataa tcgtccttta ctgtattcga gctatatagc
    19501 tgtacagtct cataatcata ctgaagagac ggataattat aaccttcacg taaagtaata
    19561 tacgtgttta caaaataccg cccaagtgct tcagattgca ggatcttttc ctgtttaacc
    19621 tgagtaatca gttctgtacg gcctgttttt tcgccaacag ccacaatagc aagctctttt
    19681 tcttttaatg gcacaagaac acaaattgca gccccaagac ataaggacag taatgatgct
    19741 gccactgcaa cacgccatgc atttttctta gaccgttcat tttctgcata gagttgtctt
    19801 tcgaaggatt tagctgtctc aatcttcttt tcgatttttt cactcataac tgattacctg
    19861 gctgtggtta atgggttcca gttcgccgga aacatcagaa ggaaggttat tccttgtggt
    19921 acatccggaa atctgaaaca ggaaaaacaa aagaatcaca catcgcataa attcacctta
    19981 ttaagcgttc acgaacacct ttaagagata aaggccaaga tgaagaaaaa gaatcccacc
    20041 aataagaagc aaaaagcgaa gatccatacc ctttctccat ttccccgcaa gccctaaacc
    20101 aagtcttatt cctgcgaagc aggtaaacac tcctaaaaga aggattagga caaaataaag
    20161 tatcatgaca aaataatgaa gcatgtcaca accagctctg actgattcca cctaagacct
    20221 taaataaggt aattcccgtg attaaaataa caagctgggt tattagcttt ctcttcctgc
    20281 tccatccgga ggtgatatgt ttatcaacga acaccagtat ccagttcagg gacatcacat
    20341 aaaaaaaacg tttcattatt ttcatgcggc gttcctcgct cgtgcttttt ccagggatgc
    20401 aagtcttctc gccttcatcc ccaaagcacc tgtcggagcc agcgcctgat atcccttttc
    20461 acccgcccat tcagcggctg ttcgcgcacc acgccccatc agattccctg ctttccctga
    20521 taaagaattc atgcccgttc ttccggccat ccctaagcca aaacctaaac cagcacgacc
    20581 gccaagcatc agagacttac cagtagccat accagcagca cccagcccca tcatggctgc
    20641 gccctgaaca gcaccttcaa cacctgcacc tgctaactgg acagcaaaac ctttagctat
    20701 aagaaccaat aaagccccca aaaacccagc cataaagccc atagcacctg tagatacaag
    20761 atttacattc cctgtttgtg cagcacgaat agcatgggaa aggatcatcc cttgaaaatc
    20821 cattgctatc cgaatcacta aactggcaaa tagaactgtc agaatactgg agaataacga
    20881 ttgtaaccaa ttattaaaca tgctccgaat aaaaccaaac atgagacaaa agataaatat
    20941 cggtgctgta ataatcagga gtttcatcgt tacatctgct gttaaaaaaa caaccacact
    21001 aacagccatt aaaataagcg agccagtcca tattaaaaac tgaccaacta caccttgatc
    21061 tttaacatat gttgatttat ctaaagagta tatttccgca ccaagatttt gcgtactttt
    21121 ccataagtta tctaaagttt gccagacact cacccctcca gaaaaaccat ctttcatgcc
    21181 ttgcaacgca tcagtagccg cagttaaata cccatccgca ttagttataa acatgattat
    21241 gattgcaaat tttgatagat cccatactaa atcctgcaag ggcgtctgag ttttactggc
    21301 aagtatttga tatcctttcc atagtacata taacgtaacc gataccgttg ctaacgacat
    21361 aatatcatta gcaaattgtg tttgataact ggagctgatt ttatctaccg aatccataac
    21421 ggtagaatta tatttaacga aaaacccctg agccatatat ccacctttta atcaaaagtt
    21481 aatggttcag cttctttctg ctgtttaata aaggcctctt gttcttgtgt gaccaacatc
    21541 tcatgatctg ctttattttt attattccag acatcatact gcatttttat cgcctgcatt
    21601 tttaacgact gcgcctgtaa tgcgttagcc aaatcctggg attctttgat atcctttgaa
    21661 gcctcaattc ggctacttaa actctgaatc tgagacattg cacttgataa ctgcttattg
    21721 atctcatttc cctgttcaat actggcagcc gttgacacaa tccgtgcttt acagagatta
    21781 tcattacgtt catagcccgt attacacata tcgaaggctc catacttgct gaacagattt
    21841 ttagcctggc tgctcaatgc accttcggga ttactggtaa aatcagaaat ataactgttc
    21901 ccctgcttat aaatatttgt cagttctgat tgtagattac ttagttcatt tgtgaatgcc
    21961 gcaatattcc ttatacctgt tgccgtggct aactggtctt tatatgcctg aatctgactc
    22021 tgataatgtt gcgcagtttc ggaccattgt ttgagtcgtt ctgtccatct tgcggcttca
    22081 acagcccatt ccggactggc atcaatagca accggaattc ctgcgctact ataaaatgaa
    22141 gccgtcaata atgatatcag catcaatgtt tttacacgtt tcataaattc acctgtcaga
    22201 ttgcccgttc gagatattca ggaacccagt catcaggctc cattccttca tgataaatac
    22261 tttcaaaaat ttccagatta tctgcactgg cagacaatat tttcagatat ccaccaagtc
    22321 ccgataaatc gagtgtcgca agtgctgaaa atgatttacc atcccccttt ttaagactgc
    22381 tttttttaat caggaactgt ctcgacattg gatcaagatt tttaacaata ttaaattctt
    22441 ctggtgtcag cttaagccct tccacataat cattgtaatc ggcatcaggg ttagccagat
    22501 aaatactggt gctgcatact tcaaccacag cccgtgaaat cttattcttc agtatttcat
    22561 ccggagactg agttgccgga ataaccagcc cgttcagctt acgaattgtt ttcagcttgt
    22621 tatagacaaa gtcgctgaat gcttcatcct gtagccattt ccagaactca tccaggaaga
    22681 taacaagacg ccgaccgtcc agaagctgcg taatgcgata cagcagatag aaagtaattg
    22741 gtgcacatac catcggatca tcaagaaact cggtgccatc aatgccaaaa ttattgacat
    22801 gctgaatatt gaatgtgtct ttagcattat caaaaaccca gccatgagcc tgaccattag
    22861 cccattggga caaacgaagg ataatcccgt tctcctgctc atcccgatca tctctctgca
    22921 taagatgctc aagcattctg gtaatgccat attcccgcat ttcgccagga ggaaaatcca
    22981 tcacagcatc aacactttcg ctaataagca atctttctct tgtcgataat ctttcaccat
    23041 ttctggtaca cagaatctcc atcagttgct tgacaaaaat acggttacgt tttgttgcct
    23101 ccagagcaaa aggattccat cctgtcggct caccgctttt tacggtgtaa tattccccac
    23161 caagcattcg gatacataat tcagcccctc tgtccttatc aagaaagaca cacgttaact
    23221 tcttattttt tgcagaatca gcaaaagttt cggggttatt gtatttctgt aatgaacagg
    23281 caagatatga cagaaacatt gtcttacctg aacccgccgt tcctataact ttagtattag
    23341 caagattctt ttcacctgtt tcatctttaa acagtactga gttatgtaaa ttcagataat
    23401 acgcctgttt gcttggtgtt ttgagaatag caaccgcttc tgtccagcag tttttatcgc
    23461 gtttcccctg gtagaaatta tggaatgatg ccagttcaac aaaatttaca ttcgataccg
    23521 gagataaacg cggccttaaa tgaaaaaccg ccggaagttg ggcaaaatat gccgctggca
    23581 atgacatggt tgacaatgcc ggaataatgc ccaggtcagt aaagtctgcc gtaattttac
    23641 ttacagattc gtcaagctcc ctgatactgt ccgccattac cattagcgtg aaatggtagt
    23701 aaccaaagga tatatcaccg gacacaatat cattttttgc cttttccagt tcctgcaatt
    23761 gagtaaccgc atcatcttct gtactcttta actgtttttc cgcacgttta atggtactca
    23821 atgcttctga tttgctcatg gatgtatatg aatgcgtaat aatatagtca gcatcagaat
    23881 actggagcac atcaaaaaca ccggatgcag tctcactaca gaaatcttta atttcaatag
    23941 aacgaaagaa tttatttcca tcaatgcggc atatctgtcc ggtatcatga ttaaaaaata
    24001 catccactcc accaagaacg ttatacaccg gagaatcagt gactctgatt ttttgcaact
    24061 tccctgtcag aagataatta tagaatgaca gttgagaaga aaataccttt ccgttctcct
    24121 catacatacc aagaagttta caggtaaatt tatcaagagc gccagagaat gtattaatca
    24181 tttcattcat tctcttcaca tgaatgttaa tatcatcttt cttttcttta atactttttt
    24241 tgcgtttttc cagtttttct acacgcccat ctggtctgta gatcagcgta aagtaaagag
    24301 tgttcccctt gaatttattt ttcttcatcg aaccatagta acaatctgat attatatcag
    24361 catatttatt acctgatttt gtggtaaagg catcataaaa actcgccctg catgaatggt
    24421 tataaaatga aacgggcagt ccggaaaatg aacgaacaag agtggcaacc tgtgagtcaa
    24481 tagtttctaa catctccgca gagattgact caaagttaat ccccatcagt tgccatgtcg
    24541 ctacataatc accgttcttt gttttgacaa tatttttatc aacatggctt gaatagggta
    24601 tatatttacc agtagtgatt cgctcattca gtttcatggc attaacaaac tcatcaattt
    24661 caacagcttc atattgtccg gagaggaacg ctcgtgcgcc aaaaaaacga ttacacactg
    24721 agtttccgag cattttcagc ttaagaaagt aaaggttaaa aatgtggtcg tcctgtttag
    24781 tgatcattct gagaacaaaa accgcgacag gaatagccag ccagatcagc ttgctgatat
    24841 aaacccccgc cagaaataac gcccctgcaa ccacagtaag agggacgaca ggaacaccca
    24901 catacatagc agggcgtgtc atcgctttat aaagtgtaga catactatcg tccccgcttt
    24961 attagttaac catcattgat gcaacttcag ccgcactggc gataacaatt gccccgatga
    25021 tgataggtga gcattcacga atcgtgcttc caccaaacag aaccttgtag ccgacccaca
    25081 gacaggcaat agtcaccgtg accagtgaaa ggccactaag ccctgtttta accttttcca
    25141 gcagtgtttc cgcttttgaa aatccatccg caaaggccgg tgatgcaact gttgccccgg
    25201 cgactgccat aactacctta tttttacgct gcattacagc agtgacagat gaactgatac
    25261 gattacatac tgatttctta aatgacattt tataagtcct gttaattaat aacttttgtt
    25321 ggatgtgagt tatcaacaaa agcagatttc agaatggttt cgggccagat aactgacgca
    25381 ttgtgtgaac gattttttcg ctgttctccc ccgttgcttc ttgtacctgg tacaacatat
    25441 tttttttcgt tccctgtata accaattcgc tcaacataac ttgtattatt aaattctttt
    25501 tcttttcgct taccgttact aaaatttcct gtatagtaac aactaagcgc attctccagg
    25561 ctcctccctc gtttataaca atccacgata atcttttcga aaacagacaa gttttcacac
    25621 gaatcaagga gtttttctgc ggacgtggaa taaaaagaaa aattactgct ggtaatctgc
    25681 atcaatccga cagaataacg tctttttctg ttttcaattt ctctgataac tgttagagct
    25741 tcctccttac ttttgggcat atgactgata acacttttgc catagctacc gcgttccctt
    25801 tcaggaataa tttcagccac agcataagga ttgaagccac tctcgacccg tgcaatatca
    25861 tttatcgtat ccggatgaac tgacgccgca cacagcaaca aggctgggaa taaagacgcc
    25921 atagctaacg ctccatatat ccatcaatac gcatactctg cattaataag taaataattt
    25981 gcttcctctt actaagcaaa gatgttttcg ttttctcttc gataaatgca ttcaacctgg
    26041 gatcgagttt tatcgtgaca taaacccctt tttccgatga cttatctgat ttctccttca
    26101 tttaatcaat ccactataag tgtgttttgg atttgtttta gatgcgaaca tgattcgcaa
    26161 aataacaatg ctcaacaata cgatcaaata acattgacaa aatggtcata ttatgattcg
    26221 tgtgatcatg caaaaaaaag cgcccatatg ggcgctctag tatgattgaa acattagtta
    26281 atcgaatgta gggactggag catccttctg agactgaata aacaatgctt gtttaacatt
    26341 cttacagttc tgagaatcag agccagactt ctcacactct gcctgctttt gtttagcttc
    26401 cgtaagatgc tgcccccacc aatccacact ttttacttct tcactacaac caacaacgaa
    26461 gaacggcaaa gcaaaaagca gatatttttt cataaattcc ccctctcact gatcctggaa
    26521 agacttgaca cgagaagggc attagtccga atgagtacca gtgttctgat ccctgccaac
    26581 gagacacctc ccagttcagc tcttacagaa tcgagatatt ttctgacagt tgaaggcttt
    26641 agtcctttga aatcagcaat ttcattcagg gtatagccat aagcgtacat cagaataatg
    26701 tctttttgtt gctcactcaa atgttcttca tcactaaaaa tttgatgaag tccagaaatg
    26761 cagatagcat catgctcagt catgataacc tccctcgtag gttgttgtga tcagcaggtg
    26821 agcaaagtag ccgctttgtt cacctgcgcc aaaatcaaat taaaatccca ggcctttcca
    26881 gttcctctct tgctgtattc ttagtttttt atgttcttct gaatcctttg taaacccgtg
    26941 ttcaagagca aacccgatat tgtgaccttg ctcatgtttt ctggatagct gctttttaac
    27001 gtcgctctct tccggctgcc tggtttcacg ctcatgaacc tgacgttctc tgttccgctc
    27061 aagggcaata ttttcaatct gccagttatt gcgatgcgtt tccctgtagc ctgcaacagt
    27121 tccatcatca ttaaacattg gactacgtac aagcgtagga gccagttttt ttactttgat
    27181 aagcgttcct ggctggagct tttcagactc gaaatgattt ttcaggtctt taccccaaat
    27241 cgtaacctgt tttccacctt ttaaagtttc ataagtgagg aaaggtgttc gtttttcccc
    27301 tgccttattc tgatatgaag tctccccaaa atccaccacc ttgtaaacat tccggagtct
    27361 tttcggattt tcagcctgta atcgctcaat ctcccgttta cgttcgggat cttttttcca
    27421 ggtagcggta acatcatatc ccataccttt cagttttcta tgaaaaccgt ttctgaactt
    27481 cctggtttca gatttagtaa gtcttaatct tcttcctgtt tcttcatcac gaagtttaat
    27541 attaacatgt acatgaggat gatcttcttt atcatcatgg tatacggcaa cgaaagcatg
    27601 gtttggataa gtctctttaa gaaattctac cgtagctttc agagcgtctt ccctgcttac
    27661 tccagcttct ggtggtggtg aaaagaccat gtttttcaca tgatcgatgt tttctccacg
    27721 atatgtcctg gagtaatcat ttcctttaga taaagttgaa gtaaactccc tattaaagtc
    27781 ttctcctttc cctgtttgct ctgaaccttt tccatcataa cagtaagcct caagttcacc
    27841 attacgggta atgtaatcaa taccattttt gatgcctgcg gctgttttac ctgagccagt
    27901 gattttaaaa gtgacttctt ttgtaggtgt ccaggtatat gctttcttgt ccgcaccatg
    27961 tctgacttta tgattaaaag ccgatttact ggcatgtata tgtcttcccg ataacttagg
    28021 agatttcgga tcacgcccag tccctttagc gcgtttaata cggtattcct gttctacata
    28081 cacacccata aatcacccaa atctgttaac aacactcttg gttaacgttt caagttttga
    28141 atcaatttgc ttacacagtt caatcacttc aataacgtct tttctgaaat ctggatcgtt
    28201 aaccgtacag aatctttcac cttttatgat aaaatgaatg ttgcgaccaa cacgatctac
    28261 agcagttgat agttttttaa gttccttaac ttcatcaggg taaagacaga cttcactctt
    28321 taacaaagac tgacgcacac gaatagcaat ttctctgctc atgctcatgc cgtgaagagc
    28381 agcattacgt ttaacaagtt ctatttcatg agggtaaaga cgcactgata ccagctttgc
    28441 tggttcatta ttaactggga tgttaacagg cttggtttca atattaagca cgttatgctt
    28501 agcaaaagca ttgatactgg taaatccact taaaatagcg ttatgttctg caaattttaa
    28561 ctcatcatca cttagcctga acgctatcat cttcataagt caccccaaca gagcctgcgg
    28621 caggcacagg caaaaacatc gtttttggac aatctgattc aaatgttaac atttgacatc
    28681 ggattgtcta tcctgcattg attgtaccat atgtggcact gtgcctggct ataaaatcgt
    28741 cgctgcatat gtccagattt cgttgctgaa agattctttc gtcttcgacc atcaggcaaa
    28801 cgtatagctg aaagatttgt gaaagaaaaa acaggcctgg cagggctggg aattgttacg
    28861 gctgaagccg taacgctttt taagtcctcg ctgcgctgcg gggcttccgg cgcaaagcgc
    28921 ctcccatgcc cttacagggc ataaaaacaa gggcaattaa gcccctgcgc gatgattgct
    28981 gacttctttt tccagagcct tattaacaaa ggcattcaac gataaatctt cttccatcgc
    29041 catttctgct acccgacgat gcagttctgg atcaagcctg acgttaaaca cgcctttaaa
    29101 cggggtatca ggttcctttc catcctccac acaagactgt aaatacagat caacagatgt
    29161 tttaaactcc tgttccagtt cagctaatgt agaagcctca taagttacca ggtcccgaat
    29221 aaacgccagt tttccataca ggacattatt ttcaaaatcg ggttcaaccg tgcctaaata
    29281 tcctttatat tttaaatgtt tcataataat ccagcctctt tcagattctg tttaattgct
    29341 ttcagcgtac cacctttgat ataactttcc ggatgtggac gatgcattaa tatggtgtga
    29401 ttgatttcag cattgaaaaa ccgcactctt gacccctgca tttccttttt gacatatccc
    29461 aaagaggaaa acaaaacgac cagttcatcc cattcaaacg tttttttact gtttaaaaac
    29521 tttgccagta gcttatctgt tttccccata aatccatccc ctatagatat gcaactaatt
    29581 atagttacac atggcatttt tgtcaaaaaa tacaaagggg cagaaagccc ctttttttat
    29641 ttaaaacatt tcataccctt gcaagtagtt taaccaccac cggaattgat cccattcgct
    29701 aaatttcatt gccggaattc tggtttccca gcctttgaca agggaatcag caagctgtac
    29761 gatctgccgc gttgacattt aaaaacctca ttcagttttg attgtcgata acgtgatcag
    29821 gttggtggaa caacctgatc acaccttgaa tagtcagtca ttaccctcct tcttttctat
    29881 agttactgtg tatccgtaaa gagtcaaaat gcgataagca tccaacgcct cacgctcact
    29941 tttaaatgtt ctgacttcgt gtaatccttt acctttatca agggatacac aaaactcata
    30001 atcttcttct acttctatct ttttaattaa tttatcaagt tcggctaatg ttcgttttgg
    30061 ctcaaaaaga aatgtgcatt gtacaatggc gtttaaatct tcccgatcaa gctctaacag
    30121 agcatcatta taagcagctt taaaacagcg attattacgc agatcagatg caaactgcat
    30181 tgcggcttgt tttaacgttt tcatgaattt cccttgcatt gatgttaata gacaatttca
    30241 gaatcaatat tgaaatttcc agaaaatgca gggatgcccc tgcattcata atgcttaaca
    30301 aagacactca ataaaatctt taaaactcaa gcattcatcg ccatctttta atgaatcaaa
    30361 atatttttca tattcgtccc tgtagaaaag gactaaatca acatcactct gcttaagttc
    30421 gccaaactga ggaacaattc cggctttata gtcacgaatt ttattgttat cagtaaatat
    30481 gacaacatac tgttgtccag tatccttatg cataaggata atagtgcaaa gcgttactcg
    30541 ttcgaatgtg aaaacatgat tagtggttga agtaaaaaca gatgagcatt tcattttaaa
    30601 tctcctgccc tgctgggcta ttgtgagagg ggccattccc ctttcgatga aacaataata
    30661 cgacctttaa gtcgtatttg caagttttct cccgtttttt tactcgataa gtgattgtta
    30721 tcacgttttt taatccgcgt aagcggatgg aaggggcgaa cgaaaatacc agggcagcac
    30781 aggagccaca tcgccccgaa gcgcggcgca gccgctgacg aacgtaccag ggtgcacagg
    30841 ccatcccttc accctgcaaa gcccaactga ccagcaggcc atgattatgc tgatttgcgc
    30901 cgcagaatgc cgcagttagc gcgagcgcaa gcgagtttac cccgccctgg tgcattgcag
    30961 catggcgggg cgctgtactg cgcgtgtgag cgtcagcgag ctacgagcgc aactccaggt
    31021 actaatataa aaccatcaca ggtcatttta ttttgactcc cttttgcaga gcacgtattc
    31081 aggatgttct cctgcaagta acaaaagaaa ctcaaactca accagtgaca gactttgact
    31141 gttttgagta ttaacttcct cttttttctg ccaggttctt agcccaacac cataaatatg
    31201 actgcattcc ttttgtgtta atcctgcttt taaacgtaat ttttttacat tttcaggtgt
    31261 attatcgggt ttcatttaac ataaactcct taattaaatt cttaactggc gtctcatcgt
    31321 cttcccttaa tccggcacac gtaaaatcaa tatgttgtaa ttcattctca ttcagtacag
    31381 catcatacaa tgtaattttc gtagttttgt tcccgatttt aacatttgtt ctggctttta
    31441 tcctttttgt aagcctgtaa ttgtcacctt ctttttcaag aaaataagtc acatcacctt
    31501 tattttttct cataactgtt tcaaataccc tgtaatttta acaagttgat ctatactcaa
    31561 gccccgaatc tgtaaatcat aactgtcgcc ctgttcggtg actttaccgt tcaatccgtt
    31621 ttcagcttta aagcctgtca atgccccata ccagctttca agctggacaa gttgcgataa
    31681 catacctttc aggatcagaa tttcgtcctt tttttccacc tggtgtttct catactcaat
    31741 cgcctgtaac acctcctgcg aagcatttac cagaattttt cgctcgatat cggctgcaat
    31801 atcaacggga ttccgttttc tggataacgt gcaaacatga tgcggactac gccaggaacg
    31861 actatccaca ctccccacca cagaaaagcg gtctttttcc atccgaatat gcacagagta
    31921 atttttaaac tgcggtgacg tcagttttat acgagacgaa caatcatcaa gtttgttcac
    31981 gcgccagccg ttccccagga tacggcagac aacttcaaag acagggtgat attgttcaaa
    32041 aaaagagttc atctgtttcc ctttttgcta cgtggctgct gttcccgtct ttttgggttc
    32101 gatgccccaa aagacgggct ggctataata tcgggggctt ctcgcccccg ttctggttac
    32161 gctctggttt ccacgcgttg ccaccctgtc gccctgcgct gtccggttgt accgtatttt
    32221 gcgcggattt cttctatcgt gtgttcttca cgattccagt aacttttatg ttcgtctgga
    32281 cgataaaacc attgtttttt ctttgccgcc catttacacc cgatttcttt taaagtttcc
    32341 ttatgtgtaa tggtttctcc actaatccag acccagttac ctatcacttc aaataccaga
    32401 ccagataaac cagaaagaac gtttaatact ttttccaggt catcaccgta attatatcgt
    32461 gcatgttcat cagtgctctg gaactgattt attttatcaa tatttgccat caatacatca
    32521 aaagctgcat ttaccgcttt catcagttca gcccctaacg ggttacgatc tggatgatat
    32581 tttaaagcgg cttttctgta tgctgctttg atatcttttt cagttaattc gccggataat
    32641 ccaaaaacgt ttaatgcttc ctggatattc atttttttaa tctcctgccc tgctgggcta
    32701 ttgtgagagg ggccattccc ctttcgatga aacaataata cgacctttaa gtcgtatttg
    32761 caagttttct cccgtttttt tactcgataa gtgattgtta tcacgttttt taatccgcgt
    32821 aagcggatgg aaggggcgaa cgaaaatacc agggcagcac aggagccaca tcgccccgaa
    32881 gcgcggcgca gccgctgacg aacgtaccag ggtgcacagg ccatcccttc accctgcaaa
    32941 gcccaactga ccagcaggcc atgattatgc tgatttgcgc cgcagaatgc cgcagttagc
    33001 gcgagcgcaa gcgagtttac cccgccctgg tgcattgcag catggcgggg cgctgtactg
    33061 cgcgtgtgag cgttagcgaa ctacgggcgc aatgcaggag aactaacaga acctccctaa
    33121 aacgctctga atcgcatttt aagcgatttt tttcatttcg catggttttc ctcatggttg
    33181 tatcgaacga agcggcagag cgcgattcag ggcttcttag tggaagatcc aaaaaggatc
    33241 aaataacgga tcatatgatc tgaatctctc tggttttgag aaaggagata ccgctcgccg
    33301 cagccgaacg gcaggagcgt agcgactgag tgagcgagga agcggaattt ttgcgatgtg
    33361 acattaggat cagtacgcac gaatattata tttatttcaa taagttaaag ttaactaggg
    33421 acaaatttat ggtcttgaaa attgagatac ataaggcatc aaatattttt ttgatgattt
    33481 tatgttattg aaagttatga gttttaatga ttgatgatgt ttgttttagc ttgcgggtaa
    33541 taatcatcgg gggacagatt tatggtaagg aaccatcatc gttgctggtt ttgttgagtt
    33601 atccacagtt cctgaatatt cgcacgcatt aaaagcctta tcttttcttg tttttcttaa
    33661 aaacttaaaa acttagagta gctctaattt attgatttta agcgtgtttt ttgtctaaac
    33721 atgagagctt cctcggttaa acatgataac ttcctcggtt aaacatgaga gcttcctcgg
    33781 ttaaacatga gagcttcctc ggttaaacat gagagcttcc tcggttaaac atgagagctt
    33841 cctcggtgat atccacaaaa tgtgtcataa gaatactaat tcgttgttct ttctggtgaa
    33901 tctattttga atctgttgtt aatctgcctt gaatccagtg tggattttga gcatggatta
    33961 cctttgtgag ataaatcaca gatatgaaac atgagaagat actcggttaa acatgatatt
    34021 gattgtgttg taatatcatg tttctggtgt taaagtctga ttatcaagag tgttggaaca
//
