LOCUS       OZ026875               33304 bp    DNA     circular BCT 10-APR-2024
DEFINITION  Escherichia coli isolate B30FEP genome assembly, plasmid:
            pB30FEP-TC-COL-2.
ACCESSION   OZ026875
VERSION     OZ026875.1
DBLINK      BioProject: PRJEB74339
            BioSample: SAMEA115456631
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Doublet,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) INRAE Nouzilly, UMR1282 Infectiologie et
            Sante Publique, Batiment 213, Centre INRAE Val de Loire, 37380
            NOUZILLY, France
FEATURES             Location/Qualifiers
     source          1..33304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="B30FEP"
                     /db_xref="taxon:562"
                     /plasmid="pB30FEP-TC-COL-2"
     gene            1..873
                     /locus_tag="PB30FEP2_2330"
     CDS             1..873
                     /locus_tag="PB30FEP2_2330"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_012599833.1"
                     /note="WP_012599833.1 RepB family plasmid replication
                     initiator protein (Escherichia coli UMN026) [pid:40.5%,
                     q_cov:95.2%, s_cov:87.1%, Eval:4.7e-67]; Prodigal_233"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="CAL1237221.1"
                     /translation="MRMTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQIN
                     SKNEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDL
                     GFKFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLS
                     VVRLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYP
                     AFKRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNM
                     FDKMYPPEN"
                     /name="RepB family plasmid replication initiator protein"
     gene            886..1290
                     /locus_tag="PB30FEP2_2320"
     CDS             886..1290
                     /locus_tag="PB30FEP2_2320"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_232"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237224.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            1425..1952
                     /locus_tag="PB30FEP2_2310"
     CDS             1425..1952
                     /locus_tag="PB30FEP2_2310"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_231"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237227.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="hypothetical protein"
     gene            complement(2343..3089)
                     /locus_tag="PB30FEP2_2300"
     CDS             complement(2343..3089)
                     /locus_tag="PB30FEP2_2300"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_003112760.1"
                     /note="WP_003112760.1 hypothetical protein (Pseudomonas
                     aeruginosa PAO1) [pid:32.2%, q_cov:81.9%, s_cov:72.7%,
                     Eval:1.9e-21]; Domain:cl00474:PAP2_like N/A. This family is
                     closely related to the C-terminal a region of PAP2.
                     [Superfamily, Aligned:25-242, Eval:2.8e-19, score:81.1];
                     Prodigal_230"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237230.1"
                     /translation="MGGGVGFVMPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVA
                     DWFYLGMGHWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKW
                     LVPVTKFRVRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRN
                     TIPDKCFPAAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSH
                     TVVSMMLAWAMSAGLAWVFFKKGEQV"
                     /name="hypothetical protein"
     gene            complement(3137..4759)
                     /gene="mcr-1.26"
                     /locus_tag="PB30FEP2_2290"
     ARG             complement(3141..4759)
                     /name="mcr-1.26"
                     /gene="mcr-1.26"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.26"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4856..5560)
                     /locus_tag="PB30FEP2_2280"
     CDS             complement(4856..5560)
                     /locus_tag="PB30FEP2_2280"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001279526.1"
                     /note="WP_001279526.1 hypothetical protein (Vibrio cholerae
                     O1 biovar El Tor str. N16961) [pid:45.4%, q_cov:82.1%,
                     s_cov:68.1%, Eval:5.7e-58]; TIGR:TIGR03899; TIGR03899:
                     TIGR03899 family protein [Name:TIGR03899, Eval:2.0e-27,
                     score:94.3, bias:0.3]; Prodigal_228"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237236.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLQIINTLKCIFSMGLRND
                     CLIKFLKYFCIA"
                     /name="hypothetical protein"
     gene            complement(5721..5990)
                     /locus_tag="PB30FEP2_2270"
     CDS             complement(5721..5990)
                     /locus_tag="PB30FEP2_2270"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_227"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237239.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            complement(6065..6712)
                     /locus_tag="PB30FEP2_2260"
     CDS             complement(6065..6712)
                     /locus_tag="PB30FEP2_2260"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_012599835.1"
                     /note="WP_012599835.1 peptidyl-arginine deiminase
                     (Escherichia coli UMN026) [pid:35.9%, q_cov:99.5%,
                     s_cov:97.7%, Eval:5.9e-34]; TIGR:TIGR03371; cellulose_yhjQ:
                     cellulose synthase operon protein YhjQ [Name:TIGR03371,
                     Eval:5.0e-09, score:34.0, bias:3.9]; COG:COG1192:BcsQ
                     Cellulose biosynthesis protein BcsQ [Category:N,
                     Aligned:1-202, Eval:2.2e-12, score:61.4]; Prodigal_226"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-arginine deiminase"
                     /protein_id="CAL1237242.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="peptidyl-arginine deiminase"
     gene            complement(7126..7305)
                     /locus_tag="PB30FEP2_2250"
     CDS             complement(7126..7305)
                     /locus_tag="PB30FEP2_2250"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_225"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237245.1"
                     /translation="MDFMSILGRCLLLPFPVAMFVGCLFPGIDDRLSGMLMSVAVGCLS
                     WYVTRPKNRQTKAA"
                     /name="hypothetical protein"
     gene            complement(8253..8957)
                     /gene="tnpA_IS26"
                     /locus_tag="PB30FEP2_2240"
     CDS             complement(8253..8957)
                     /gene="tnpA_IS26"
                     /locus_tag="PB30FEP2_2240"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:ISFinder:IS26"
                     /note="ISFinder:IS26 IS6 family transposase IS26
                     [pid:100.0%, q_cov:100.0%, s_cov:100.0%, Eval:8.3e-179];
                     COG:COG3316:Rve Transposase (or an inactivated derivative)
                     [Category:X, Aligned:1-214, Eval:2.2e-72, score:217.0];
                     Prodigal_224"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase IS26"
                     /protein_id="CAL1237248.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="tnpA_IS26"
     gene            complement(9044..9508)
                     /gene="hns"
                     /locus_tag="PB30FEP2_2230"
     CDS             complement(9044..9508)
                     /gene="hns"
                     /locus_tag="PB30FEP2_2230"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:SPE04298.1"
                     /note="resMGE:SPE04298.1 global nucleic acid-binding
                     transcriptional dual regulator (Escherichia coli IncX
                     plasmid) [pid:96.1%, q_cov:100.0%, s_cov:99.4%,
                     Eval:1.7e-96]; Domain:cl27638:Histone_HNS H-NS histone
                     family. global DNA-binding transcriptional dual regulator
                     H-NS; Provisional [Superfamily, Aligned:19-145,
                     Eval:3.4e-21, score:80.6]; Prodigal_223"
                     /codon_start=1
                     /transl_table=11
                     /product="global nucleic acid-binding transcriptional dual
                     regulator"
                     /protein_id="CAL1237251.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="hns"
     gene            complement(9729..11882)
                     /locus_tag="PB30FEP2_2220"
     CDS             complement(9729..11882)
                     /locus_tag="PB30FEP2_2220"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:UniProtKB:B7LJB3"
                     /note="B7LJB3 DNA topoisomerase (Escherichia coli UMN026)
                     [pid:98.2%, q_cov:100.0%, s_cov:100.0%, Eval:0.0e+00];
                     TIGR:TIGR01056; topB: DNA topoisomerase III
                     [Name:TIGR01056, Eval:6.3e-161, score:535.2, bias:0.0];
                     COG:COG0550:TopA DNA topoisomerase IA [Category:L,
                     Aligned:1-622, Eval:4.6e-147, score:438.2]; Prodigal_222"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="CAL1237254.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="DNA topoisomerase"
     gene            complement(11887..12459)
                     /locus_tag="PB30FEP2_2210"
     CDS             complement(11887..12459)
                     /locus_tag="PB30FEP2_2210"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_221"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237257.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            complement(12770..13075)
                     /locus_tag="PB30FEP2_2200"
     CDS             complement(12770..13075)
                     /locus_tag="PB30FEP2_2200"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000820911.1"
                     /note="WP_000820911.1 IncN plasmid KikA protein
                     (Escherichia coli UMN026) [pid:81.7%, q_cov:92.1%,
                     s_cov:93.0%, Eval:1.1e-51]; Prodigal_220"
                     /codon_start=1
                     /transl_table=11
                     /product="IncN plasmid KikA protein"
                     /protein_id="CAL1237260.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="IncN plasmid KikA protein"
     gene            complement(13059..13478)
                     /locus_tag="PB30FEP2_2190"
     CDS             complement(13059..13478)
                     /locus_tag="PB30FEP2_2190"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000722607.1"
                     /note="WP_000722607.1 hypothetical protein (Escherichia
                     coli UMN026) [pid:34.5%, q_cov:84.9%, s_cov:86.9%,
                     Eval:7.0e-15]; Prodigal_219"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237263.1"
                     /translation="MKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFHG
                     VIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKMEG
                     ATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="hypothetical protein"
     gene            complement(13490..15352)
                     /locus_tag="PB30FEP2_2180"
     CDS             complement(13490..15352)
                     /locus_tag="PB30FEP2_2180"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_012599838.1"
                     /note="WP_012599838.1 conjugal transfer protein TraG
                     (Escherichia coli UMN026) [pid:40.0%, q_cov:93.9%,
                     s_cov:93.5%, Eval:3.8e-131]; TIGR:TIGR02767; TraG-Ti:
                     Ti-type conjugative transfer system protein TraG
                     [Name:TIGR02767, Eval:8.0e-26, score:88.9, bias:0.3];
                     COG:COG3505:VirD4 Type IV secretory pathway, VirD4
                     component, TraG/TraD family ATPase [Category:U,
                     Aligned:72-580, Eval:8.5e-77, score:253.1]; Prodigal_218"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="CAL1237266.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="conjugal transfer protein TraG"
     gene            complement(15339..16367)
                     /gene="pilx11"
                     /locus_tag="PB30FEP2_2170"
     CDS             complement(15339..16367)
                     /gene="pilx11"
                     /locus_tag="PB30FEP2_2170"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:SPE04309.1"
                     /note="resMGE:SPE04309.1 P-type conjugal transfer ATPase
                     Pilx11 (Escherichia coli IncX plasmid) [pid:98.8%,
                     q_cov:100.0%, s_cov:99.7%, Eval:0.0e+00]; TIGR:TIGR02788;
                     VirB11: P-type DNA transfer ATPase VirB11 [Name:TIGR02788,
                     Eval:1.9e-108, score:360.1, bias:0.0]; COG:COG0630:VirB11
                     Type IV secretory pathway ATPase VirB11/Archaellum
                     biosynthesis ATPase [Category:U, Aligned:22-323,
                     Eval:5.6e-74, score:228.4]; Prodigal_217"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugal transfer ATPase Pilx11"
                     /protein_id="CAL1237268.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="pilx11"
     gene            complement(16369..17487)
                     /gene="pilx10"
                     /locus_tag="PB30FEP2_2160"
     CDS             complement(16369..17487)
                     /gene="pilx10"
                     /locus_tag="PB30FEP2_2160"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:SPE04310.1"
                     /note="resMGE:SPE04310.1 conjugal transfer protein Pilx10
                     (Escherichia coli IncX plasmid) [pid:98.7%, q_cov:100.0%,
                     s_cov:99.7%, Eval:0.0e+00]; COG:COG2948:VirB10 Type IV
                     secretory pathway, VirB10 components [Category:U,
                     Aligned:190-372, Eval:6.8e-53, score:176.5, N-term
                     missing]; Prodigal_216"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein Pilx10"
                     /protein_id="CAL1237270.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="pilx10"
     gene            complement(17480..18388)
                     /locus_tag="PB30FEP2_2150"
     CDS             complement(17480..18388)
                     /locus_tag="PB30FEP2_2150"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000976329.1"
                     /note="WP_000976329.1 P-type conjugative transfer protein
                     VirB9 (Escherichia coli UMN026) [pid:39.7%, q_cov:98.7%,
                     s_cov:98.3%, Eval:1.7e-73]; TIGR:TIGR02781; VirB9: P-type
                     conjugative transfer protein VirB9 [Name:TIGR02781,
                     Eval:3.1e-66, score:221.5, bias:0.7];
                     Domain:cl11423:VirB9_CagX_TrbG VirB9/CagX/TrbG, a component
                     of the type IV secretion system. This family includes type
                     IV secretion system CagX conjugation protein. Other members
                     of this family are involved in conjugal transfer to plant
                     cells of T-DNA. [Superfamily, Aligned:1-297, Eval:2.5e-23,
                     score:93.6]; Prodigal_215"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="CAL1237272.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="P-type conjugative transfer protein VirB9"
     gene            complement(18385..19071)
                     /locus_tag="PB30FEP2_2140"
     CDS             complement(18385..19071)
                     /locus_tag="PB30FEP2_2140"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001005327.1"
                     /note="WP_001005327.1 membrane protein (Escherichia coli
                     UMN026) [pid:41.4%, q_cov:97.4%, s_cov:97.8%,
                     Eval:5.6e-56]; Domain:cl01500:VirB8_like virulence protein
                     VirB8. This family includes the conjugal transfer protein
                     family TrbF, a family of proteins known to be involved in
                     conjugal transfer. The TrbF protein is thought to compose
                     part of the pilus required for transfer. This domain is
                     similar to the type IV secretion system (T4ASS) component
                     VirB8 and possibly has a similar fold to the nuclear
                     transport factor-2 (NTF-2)-like superfamily. [Superfamily,
                     Aligned:2-224, Eval:8.1e-40, score:134.4]; Prodigal_214"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein"
                     /protein_id="CAL1237274.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="membrane protein"
     gene            complement(19410..19589)
                     /locus_tag="PB30FEP2_2130"
     CDS             complement(19410..19589)
                     /locus_tag="PB30FEP2_2130"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_213"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237276.1"
                     /translation="MKIMKRFFYVMSLNWILVFVDKHITSGWSRKRKLITQLVILITGI
                     TLFKVLGGISQSWL"
                     /name="hypothetical protein"
     gene            complement(19586..20680)
                     /locus_tag="PB30FEP2_2120"
     CDS             complement(19586..20680)
                     /locus_tag="PB30FEP2_2120"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000513454.1"
                     /note="WP_000513454.1 pilus assembly protein (Escherichia
                     coli UMN026) [pid:37.1%, q_cov:97.5%, s_cov:90.2%,
                     Eval:4.3e-57]; Domain:cl01503:TrbL TrbL/VirB6 plasmid
                     conjugal transfer protein. conjugal transfer protein TrbL;
                     Provisional [Superfamily, Aligned:14-328, Eval:1.2e-09,
                     score:56.3]; Prodigal_212"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="CAL1237278.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="pilus assembly protein"
     gene            complement(20692..21408)
                     /locus_tag="PB30FEP2_2110"
     CDS             complement(20692..21408)
                     /locus_tag="PB30FEP2_2110"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000744204.1"
                     /note="WP_000744204.1 pilus assembly protein (Escherichia
                     coli UMN026) [pid:42.9%, q_cov:99.2%, s_cov:94.0%,
                     Eval:2.0e-62]; TIGR:TIGR02791; VirB5: P-type DNA transfer
                     protein VirB5 [Name:TIGR02791, Eval:6.4e-12, score:43.7,
                     bias:11.0]; Prodigal_211"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="CAL1237280.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="pilus assembly protein"
     gene            complement(21420..24167)
                     /gene="pilx3-4"
                     /locus_tag="PB30FEP2_2100"
     CDS             complement(21420..24167)
                     /gene="pilx3-4"
                     /locus_tag="PB30FEP2_2100"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:SPE04316.1"
                     /note="resMGE:SPE04316.1 conjugal transfer protein Pilx3-4
                     (ATPase) (Escherichia coli IncX plasmid) [pid:99.9%,
                     q_cov:100.0%, s_cov:99.9%, Eval:0.0e+00]; TIGR:TIGR00929;
                     VirB4_CagE: type IV secretion/conjugal transfer ATPase,
                     VirB4 family [Name:TIGR00929, Eval:3.5e-198, score:658.4,
                     bias:1.4]; COG:COG3451:VirB4 Type IV secretory pathway,
                     VirB4 component [Category:U, Aligned:114-913,
                     Eval:6.3e-168, score:506.5]; Domain:cl01501:VirB3 Type IV
                     secretory pathway, VirB3-like protein. type IV secretion
                     system protein VirB3; Provisional [Superfamily,
                     Aligned:3-86, Eval:1.5e-13, score:64.7]; Prodigal_210"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein Pilx3-4 (ATPase)"
                     /protein_id="CAL1237282.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="pilx3-4"
     gene            complement(24186..24512)
                     /locus_tag="PB30FEP2_2090"
     CDS             complement(24186..24512)
                     /locus_tag="PB30FEP2_2090"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000600171.1"
                     /note="WP_000600171.1 TriB protein (Escherichia coli
                     UMN026) [pid:44.6%, q_cov:63.9%, s_cov:76.3%,
                     Eval:7.1e-16]; Prodigal_209"
                     /codon_start=1
                     /transl_table=11
                     /product="TriB protein"
                     /protein_id="CAL1237284.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TriB protein"
     gene            complement(24526..25146)
                     /gene="pilx1"
                     /locus_tag="PB30FEP2_2080"
     CDS             complement(24526..25146)
                     /gene="pilx1"
                     /locus_tag="PB30FEP2_2080"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:INSD:SPE01517.1"
                     /note="resMGE:SPE01517.1 conjugal transfer protein Pilx1
                     (Soluble lytic murein transglycosylase) (Escherichia coli
                     IncX plasmid) [pid:99.5%, q_cov:100.0%, s_cov:99.5%,
                     Eval:1.3e-155]; Prodigal_208"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein Pilx1 (Soluble lytic
                     murein transglycosylase)"
                     /protein_id="CAL1237286.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="pilx1"
     gene            complement(25149..25325)
                     /locus_tag="PB30FEP2_2070"
     CDS             complement(25149..25325)
                     /locus_tag="PB30FEP2_2070"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_207"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237288.1"
                     /translation="MKEKSDKSSEKGVYVTIKLDPRLNAFIEEKTKTSLLSKRKQIIYL
                     LMQSMRIDGYMER"
                     /name="hypothetical protein"
     gene            complement(25502..25717)
                     /locus_tag="PB30FEP2_2060"
     CDS             complement(25502..25717)
                     /locus_tag="PB30FEP2_2060"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000835348.1"
                     /note="WP_000835348.1 hypothetical protein (Escherichia
                     coli UMN026) [pid:50.8%, q_cov:90.1%, s_cov:86.7%,
                     Eval:6.7e-14]; Prodigal_206"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237290.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="hypothetical protein"
     gene            complement(25714..26007)
                     /locus_tag="PB30FEP2_2050"
     CDS             complement(25714..26007)
                     /locus_tag="PB30FEP2_2050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_205"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237292.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="hypothetical protein"
     gene            complement(26084..27313)
                     /gene="taxC"
                     /locus_tag="PB30FEP2_2040"
     CDS             complement(26084..27313)
                     /gene="taxC"
                     /locus_tag="PB30FEP2_2040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_012599843.1"
                     /note="WP_012599843.1 spore coat protein CotH (Escherichia
                     coli UMN026) [pid:35.4%, q_cov:84.1%, s_cov:85.9%,
                     Eval:2.1e-62]; Prodigal_204"
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotH"
                     /protein_id="CAL1237294.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            complement(27317..27820)
                     /locus_tag="PB30FEP2_2030"
     CDS             complement(27317..27820)
                     /locus_tag="PB30FEP2_2030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_203"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237296.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="hypothetical protein"
     gene            complement(28181..28528)
                     /locus_tag="PB30FEP2_2020"
     CDS             complement(28181..28528)
                     /locus_tag="PB30FEP2_2020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_002668012.1"
                     /note="WP_002668012.1 antitoxin HicB (Treponema denticola
                     ATCC 35405) [pid:43.0%, q_cov:93.0%, s_cov:96.4%,
                     Eval:6.9e-30]; COG:COG4226:HicB Predicted nuclease of the
                     RNAse H fold, HicB family [Category:R, Aligned:1-108,
                     Eval:4.0e-32, score:106.3]; Prodigal_202"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="CAL1237298.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="antitoxin HicB"
     gene            complement(28525..28773)
                     /locus_tag="PB30FEP2_2010"
     CDS             complement(28525..28773)
                     /locus_tag="PB30FEP2_2010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_201"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237299.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="hypothetical protein"
     gene            complement(28865..29002)
                     /locus_tag="PB30FEP2_2000"
     CDS             complement(28865..29002)
                     /locus_tag="PB30FEP2_2000"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_200"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237300.1"
                     /translation="MSTRQIVQLADSLVKGWETRIPAMKFSEWDQFRWWLNYLQGYEMF
                     "
                     /name="hypothetical protein"
     gene            complement(29078..29428)
                     /locus_tag="PB30FEP2_1990"
     CDS             complement(29078..29428)
                     /locus_tag="PB30FEP2_1990"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_199"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237301.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            complement(29519..29818)
                     /locus_tag="PB30FEP2_1980"
     CDS             complement(29519..29818)
                     /locus_tag="PB30FEP2_1980"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_012599848.1"
                     /note="WP_012599848.1 hypothetical protein (Escherichia
                     coli UMN026) [pid:99.0%, q_cov:99.0%, s_cov:86.7%,
                     Eval:1.8e-67]; Prodigal_198"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237302.1"
                     /translation="MKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVIFTDNNK
                     IRDYKAGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            complement(30272..30499)
                     /locus_tag="PB30FEP2_1970"
     CDS             complement(30272..30499)
                     /locus_tag="PB30FEP2_1970"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000801440.1"
                     /note="WP_000801440.1 hypothetical protein (Escherichia
                     coli UMN026) [pid:100.0%, q_cov:100.0%, s_cov:100.0%,
                     Eval:1.2e-50]; Prodigal_197"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237303.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            complement(30486..30737)
                     /locus_tag="PB30FEP2_1960"
     CDS             complement(30486..30737)
                     /locus_tag="PB30FEP2_1960"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001230707.1"
                     /note="WP_001230707.1 hypothetical protein (Escherichia
                     coli UMN026) [pid:100.0%, q_cov:100.0%, s_cov:100.0%,
                     Eval:8.4e-56]; Prodigal_196"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237304.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            complement(30734..31276)
                     /locus_tag="PB30FEP2_1950"
     CDS             complement(30734..31276)
                     /locus_tag="PB30FEP2_1950"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001083906.1"
                     /note="WP_001083906.1 hypothetical protein (Escherichia
                     coli UMN026) [pid:100.0%, q_cov:100.0%, s_cov:100.0%,
                     Eval:6.6e-134]; Prodigal_195"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237305.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            complement(31381..31896)
                     /locus_tag="PB30FEP2_1940"
     CDS             complement(31381..31896)
                     /locus_tag="PB30FEP2_1940"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_012599850.1"
                     /note="WP_012599850.1 molecular chaperone DnaJ (Escherichia
                     coli UMN026) [pid:100.0%, q_cov:100.0%, s_cov:58.6%,
                     Eval:3.9e-126]; TIGR:TIGR02349; DnaJ_bact: chaperone
                     protein DnaJ [Name:TIGR02349, Eval:4.6e-11, score:40.6,
                     bias:0.5]; Domain:cl28246:DnaJ DnaJ-class molecular
                     chaperone with C-terminal Zn finger domain
                     [Posttranslational modification, protein turnover,
                     chaperones]. This model describes TopJ (MG_200, CbpA), a
                     DnaJ homolog and probable assembly protein of the
                     Mycoplasma terminal organelle. The terminal organelle is
                     involved in both cytadherence and gliding motility.
                     [Cellular processes, Chemotaxis and motility] [Superfamily,
                     Aligned:9-54, Eval:3.8e-13, score:63.0, C-term missing];
                     Prodigal_194"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="CAL1237306.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="molecular chaperone DnaJ"
     gene            32731..32856
                     /locus_tag="PB30FEP2_1930"
     CDS             32731..32856
                     /locus_tag="PB30FEP2_1930"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /note="Prodigal_193"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAL1237307.1"
                     /translation="MIDDVCFSLRVIIIGGQIYGKEPSSLLVLLSYPQFLNIRTH"
                     /name="hypothetical protein"
     Typing          2..873
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(26088..27313)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(13494..15352)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(17484..18388)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(18389..19071)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          complement(19614..20680)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.69e-172"
ORIGIN
        1 atgagaatga cgacaaataa gacttccctt tctcgcttaa caaaagtgag acatcgaaat
       61 gaattgaatt caacgctgtc aacattgcct atggctgcta aaaaagtctt atttttagcc
      121 atgtgccaga taaactctaa aaatgaattc gatgatgatc acatatttta tgtgacagtt
      181 gccgattaca ttaaatgggt tcaggttaag cctgatgcgg cttatcttgc tttgagggat
      241 ggctctaata tattagatac gacgcttctt aagctgaaac atgatgaaat attagaactg
      301 agtagtgatt tgggatttaa gttcactaaa agtaatgtac ctgattcaat gaatttgagt
      361 ctgactgttt tttctactta ttacaggaat gaaggtagag tggggatcaa gtttacgaaa
      421 gaagcgaaaa gatttttatg taaacttatt ggtgaagaaa aacgttatac aacgcaagtt
      481 ttattatctg ttgttcgact gacaagtgtt aatgctgcat cactatatca actcattcgt
      541 aaaatatata gtaataattc tcgcgcgaat agttttgaaa tgacaataga tgaacttaaa
      601 gatgaattga atctttatac aataggggcg gggggggtga aggattataa gtatccagat
      661 tatcctgctt ttaaaagaga tgttcttaat aaatcagtaa aagaaatcat gaaacatacg
      721 gaagtaaaga acttaagttt cgtagtttca gaaaaaattg ggagaaaagt ttataaatta
      781 aagttctctt atacaattgg ttatgagggc gataccaggg aagactcaga gtttactaat
      841 atgtttgata aaatgtaccc acctgaaaat tgaggtcata acaatatgaa ggataatatc
      901 gtagattttc aaagaatcag ggacagcatg aatgatcatt caaccgatgt tgcaaatgat
      961 gccgtttatt ctgacaaaaa gaaaatatcc aggttctttt atctgctgac acctgtcaga
     1021 taccttctgg ctgcttccct ttatctggtt gttgttattc cattggggat tcttgcggga
     1081 tttcgtttct tgttgcagtt tttaggcggt atcgcagtga tgggtttaac tatcgcctgg
     1141 tacatgggtg cagatgtcag tagttacgtt gttcttgctg catggatcgc cttaactgtt
     1201 tgtggttgcg cagagcagat ttttgaatgg tggatcgaaa gccgttttgc gttccgtctt
     1261 tttggtatcg ggagaacgcc ggaagaatga gcgccgatct tacagacagt aaagaacaat
     1321 gcaggataga caatccgatg tcaaatgtta acatttgaat cagattgtcc aaaaacgatg
     1381 tttttgcctg tgcctgccgc aggctctttt aaaaggaata tgaaatgata tttaaccgta
     1441 ataaaaaacg agatgaacaa cataattctg ctgttgtttt gccatcagct gaaaatgtat
     1501 tctctgattt tattaagaac aatccagctt tgacaaccaa tgcttttaca gccattgggg
     1561 aggttgattg ttttactaaa gtacccttac tctcaaataa tgaatcagag tttttaggca
     1621 ttctttcacg taatattata cctgattatt ccgtttttcc caaaatacgg ctcattgaat
     1681 ttgttcgtcc tcatggtagc gaggaagctg taaaagattt aatccgtgaa gtgcagaata
     1741 taacctgtga ttttgtcctg aaatcatatg atgaaacagt cctggctgtt atccagtttg
     1801 gtgaaaccgc aaccgtcaga cagcaaaaga aaaaactgat tatccagcgc gtgtgtgcta
     1861 tgacaggaat ttcttttttc gaatttaaaa acactgtcga tatgatgggg agcgaagatt
     1921 tttgtcagtt cctgcgggat gatgaagagt aattaaaaat aagcccacat atttgcgggc
     1981 ttaacaggct ctttattagt acttgctgca tttgtgatta ctggcaacac agttttcact
     2041 gggcagtttt tgcggtcgct tttaattatg gttagtggaa ctcagacagg taactcgacc
     2101 tctttacata ttaataattg atagttttac ctggcttcct tcaacattta ttttgttgag
     2161 gaatttattt aaatacgcaa tatctggagt ggaaccgcat attttcatca attcttgacc
     2221 gataggcgta agtaaagccg ttcctattgg tagtttccag gttgatgaat catcgctttg
     2281 aaactcaact gaaaaaaccg ttccgtaata accgatacga ccgtttttac ttttaaaggc
     2341 gattaaactt gttcaccctt cttaaaaaac acccacgcaa gccctgccga catcgcccac
     2401 gcaagcatca tcgacaccac cgtatggctc aaaaaatgat cgccaataat catcttatag
     2461 cagcccatcg cccagcccaa tgccatcacc acgatgatga tcgcaccacg acggcggcgc
     2521 aaacttggcg caaacgcaaa ggcaaacagc gcaaatccgc tactggcatg cgccgcagga
     2581 aagcacttat caggaatggt gttacgcata ctatcaagca tcggcaaata cggcagcgta
     2641 ccatcaaaaa tcgtcaaatg cacagggcag accacatgag tatatgcctt caatgacgcg
     2701 acaactgtcg gcactaggat caaagtcagc accagatacg caagctcacg cacgcgaaat
     2761 tttgtcactg ggacaagcca cttggcagtg atagcattgc cctttttctc atgataagca
     2821 cggcaaatgg tcgcaaccag caagtagatc agcatcgcca tcagcagctt ttttagtcca
     2881 gaatacagta gcaaatctgg caaaaaagct tgcttggcaa ccatccaatg ccccataccc
     2941 agataaaacc aatcagcgac catcagatca atctgactgt gctcaagggt cagcgtggca
     3001 acgatcgcca tgacaagagc gatactcatc tcagcaagta ggcgtttatt tgataaatac
     3061 ggcataacaa accccacccc accgcccata atacgaatgg agtgtgcggt gggtttggaa
     3121 aaaatacagg gagaaatcag cggatgaatg cggtgcggtc tttgactttg tccgcggtga
     3181 catcaaacag ctttaataat gtcggcgtga tcgcgtcatg ggtcaggacg gtatcggttg
     3241 ccattggcgt gatgccagtt tgcttatccg tccagaaaaa tgcaggcaca ctgcgctgtt
     3301 cttttggtgc aaaggcattt ggcataccat gtagatagac accgttctca cccagacttt
     3361 cgccatgatc gctgacatac agcattgaga catcataggc attgctgtgc gtctgcagcc
     3421 actggatact ttgagcgatg aaatcatcgg tggcaagcaa ggcattgtca taagcattga
     3481 tcaaggactg atgttcgcac ttggcaagct cattaccttc acacactggc gtgaatttgg
     3541 caaacttttc atcatatcgc ttaaaatacg caggcccgtg attgcccatt tggtgcagca
     3601 tgatcagcat atctttgccg ttattggcag cgacaaagtc atctaagcca acgagcatac
     3661 cgacatcgcg gcattcgtta taaggattgg tgttgcagat ggcgttgttg gtcgcggatt
     3721 tataatcggc aaattgcgct tttggcagct tatccatcac gccttttgag tccgaattat
     3781 tatcacgcca caagatactt acgcccaagc gatccagcgt atccagcaca ttttcttggt
     3841 atttggcggt atcgacatca tactcatccg cgcccagata gctgaacata cacggcacag
     3901 aatacgccgt cgatgtgccg cacgatgtga cattgctaaa attggtcacg ccatcgatct
     3961 tggcaagctg tgggaaagta tcgcgctcat agccattgaa gctgacatga tcggcgcgtg
     4021 ccgtctcacc gacgacgaac accactaggc gtggcttacg catatcaggc ttggttgctt
     4081 gtaccgcgtc tttggcgtga taaatggtat cttttggcgc actggctttt ttatactcaa
     4141 tactggcaag cttacccacc gagtagattg gcatgatcgg attgacatag ctacgcagcg
     4201 gcttatgcac gcgaaagaaa ctggcataat gactgctgaa cgccaccaca ggcagtaaaa
     4261 tcagcgcaag acttgccacg atcaagccca atcggcgcat caaacccttg ccccaagtcg
     4321 gataatccac cttaacaaaa gccacaagca aacttggtag cacacccaaa ccaatgatac
     4381 gcatgataaa cgctgcgttt aatagatcct tggtctcggc ttggtcggtc tgtagggcat
     4441 tttggagcat ggtcgtatca tagaccgtgc cataagtgtc agtaaaataa ctggtcaccg
     4501 cgcccatgat taatagcaaa atcaacacag gctttagcac atagcgatac gatgataaca
     4561 gcgtggtgat cagtagcatc gcgccaaaga gcacgacagc gatcgtcagc acaaagccga
     4621 gattgtccgc gatgggatag gtttggctga ttttatcaaa aaaggtaaga ttggcggtcg
     4681 cggtcaagaa aacggcaaca ctcgccacaa gaacaaacgg actgaccgag cgtcggtacc
     4741 acacagaagt atgctgcatc ctgagaaact actcaaaaaa taaacggtgg gataattgcg
     4801 gcaattatac tgcaattttt taagaaatcc agtacaattt atctgatata aaaaattatg
     4861 cgatacagaa atatttcaga aattttatta ggcaatcatt gcgcaatccc atactgaata
     4921 tacacttgag agtatttata atttgtaaac tataaccaat gccttgttga ataacgccta
     4981 aggagtctaa aaccgaaagt ttatcgaaag ttataccttc agtatctcca ttctcatcgg
     5041 taaataaaat tggagtgagt ttgtttgcct gccaaacaaa ctttccaaaa taagtaataa
     5101 ggttcgcttc ttctttgctt atagtggata atagttttaa ggttctacga ctaaacgaac
     5161 cttttgactt tgcttcttcg gataaaattc ttccccaaag catttgcatt tgttcgtcac
     5221 taatatcctc acactccctg aagaaagcct ctatccaatc ttcgtcaata tcagaaacat
     5281 tatcactctc agatagactt tgtgctgctt gcattgttat attttcaatg ttttcctgtc
     5341 gtttcgtttc acgtttgaga aaacgctcaa ctgcccgttt ttctattccc tcaagctcta
     5401 acctagaaat tagctctgtg cgtttagctt cagcttcagc cttcgctttt ttcctgattc
     5461 ttgtaggttc ataaagcaca ccaatagcat cacttatttt ttcaataagt tttgttcctg
     5521 gttctgataa tcctgataaa cttaaatcga taaggctcat ctagtgctcc taaatacccg
     5581 tgcaacaact gtacaagggt attttattgc aagagtaact aaaaatgaat cacaactaca
     5641 tttaaaagtg atgaagttag tacattcaga taagtatcta aaagaaaatg ataaagagtt
     5701 tattgggcca cttgctatga ttaatcattt ctgtcagctc tgaaccacaa attaatgagt
     5761 tcgttttcgt ccagatcctg ataagccagt aatgctgctt tgattatttt tgtcttggga
     5821 cgatgcagtc tttttgattc gttatcaatg aattcaagca attcattcgg tatgcggaat
     5881 gaagccagtt ttgttttgtt ctctgtgggg cgttttcctg cgtcctgaac aaattttgtg
     5941 gcgttcactt cctggttttg ttctagttct ggtagttcca gctttgccat gatgtacctt
     6001 ctaatctgat tgtttcacac taatgtatat catgatgatg tacattagtg tatgtcacta
     6061 tgatttacag ttccagttct ttcagcaaga gttcgatttg tgcttttgcc tggctggcac
     6121 cgctaacatt actttttaac tcgtgaaccc ccattccctg attcattgct cttacaaagg
     6181 cccggagaaa agaaaccgtg cttttcattg gtttaagcca gtaatcatgg ctgttgagag
     6241 agtctcttaa ttcttgttgt tcactgaacg tatttggctg gcatttatac aggagcacat
     6301 gtgcagccag cctgggattt attttttgcg catccctgac aatttttgtc acagtctgaa
     6361 gactgtctgc ttccacctgg gcaagcgggt cgatcgggac aatcacaatg tctgcaactt
     6421 ttagtgcagt tctgaactcc gtgctgtcat atccggcagt atctataatg aggatttctg
     6481 ctacatctgc catttttgtt attttggcac tgatgtcgcc ataggcttca tttaatgcga
     6541 cgggcggtaa tccggcctgt tctctgcgtt tattccattt tttggtcgag ccattcaggt
     6601 ctgtttcaac aagccccgtt ctctttccct gttgtgagag gcaaacagtt aagttggttg
     6661 ccagatgtga tttacctgta ccgcctttat caccaccgat tacgataatc atagtacccc
     6721 ttgtatgtca cagtgattta ctcatgtgta tttctatgtg atgtacgcat gcgtatgtca
     6781 catggacata ctatagtgta tgtcacaatg aattacaatg atgtacatca tgaagaaata
     6841 cattattgta tgtcagtgtg atgtacccgt tggttggtca cttgaaagca aaagtgacag
     6901 cgttactttc ggtatcctcc cttgctgcga aaccttgtgg caatacagca atgagtatgt
     6961 ttagttacaa cattcaaaat taagcgatta gattccgccc gtacgggcgg ggcgcgccac
     7021 tttctgtagt ggggaggggc agggaggtta tgtttgctgg atgagcggag gatggaatga
     7081 gctattagaa tgttgtaact aaagggtatc ccaattaaag gccacttacg cggcctttgt
     7141 ctgtcggttc ttcggtctgg tgacatacca cgataaacat cccacagcaa cactcataag
     7201 cattccggat aagcgatcat cgatgccagg aaacaggcaa ccgacaaaca tcgcaacagg
     7261 gaaaggaaga agtaaacagc gccccaaaat gctcataaaa tccataaatc ctcctttttt
     7321 gttaaagggc aggaagcggt tcacttcctg cccttagtta cgtttccgtt gtcatcaatc
     7381 gatgaattgg cggatcagtt ccagaactgc aatcagcgcg tttgcaacgg cgaccacagc
     7441 tctgagaacg atgattaaca tattaatcat ccgtttctcc agtaaaactg gaggaaatca
     7501 ccgttgcttg ctcatacact gcctgtcaca acggctctct cgcattgtta ggtgtagagt
     7561 gacctcctcc tgccagagca ccgaatacag caccacctgt ttatccagcc aggactttaa
     7621 aatcgttcat acccgcagat acgggaacag caggattgca agccaactac atgactaact
     7681 gactgattat acgctacaca aaacacccgt tacaccacta aatgtggttc cttaaccaca
     7741 tttagttgct ttacagcacc atagtgaata aaatgtaaac gttaggtgta gagtaacctt
     7801 ctccggtcag agccaccaac tcaagactgg aaaacgcaaa aaagcccgat taccagtcgg
     7861 gtttttttgt gcctgtcgtt cggctgtagt tccgtttttt ctccttcatt tgagggattt
     7921 ttctttcatt ttttttgttg cttccaatac tgcctgtcgc gttggccaac agcttgacgc
     7981 gttaccggca ttttcattac tcaatatttt tcagtgaatt aaaatgcaac agcgacagaa
     8041 aaggacattc agctttttag atgaatgcta tagcaacggt gtgctacgta aaatctgcga
     8101 aaaaagcagg ggtagggtag gttatttttc acgtagcaac gccatctggc actgtacata
     8161 ggtatgtcac acagacatac ctatgtgtat gtctgtgtga ggcactgttg caaagttagc
     8221 gatgaggcag ccttttgtct tattcaaagg ccttacattt caaaaactct gcttaccagg
     8281 cgcatttcgc ccaggggatc accataataa aatgctgagg cctggccttt gcgtagtgca
     8341 cgcatcacct caataccttt gatggtggcg taagccgtct tcatggattt aaatcccagc
     8401 gtggcgccga ttatccgttt cagtttgcca tgatcgcatt caatcacgtt gttccggtac
     8461 ttaatctgtc ggtgttcaac gtcagacggg caccggcctt cgcgtttgag cagagcaagc
     8521 gcgcgaccat aggcgggcgc tttatccgtg ttgatgaatc gcgggatctg ccacttcttc
     8581 acgttgttga ggattttacc cagaaaccgg tatgcagctt tgctgttacg acgggaggag
     8641 agataaaaat cgacagtgcg gccccggctg tcgacggccc ggtacagata cgcccagcgg
     8701 ccattgacct tcacgtaggt ttcatccatg tgccacgggc aaagatcgga agggttacgc
     8761 cagtaccagc gcagccgttt ttccatttca ggcgcataac gctgaaccca gcggtaaatc
     8821 gtggagtgat cgacattcac tccgcgttca gccagcatct cctgcagctc acggtaactg
     8881 atgccgtatt tgcagtacca gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg
     8941 cctttgaatg ggttcatgtg cagctccatc agcaaaaggg gatgataagt ttatcaccac
     9001 cgactatttg caacagtgcc ctgtgtgatg tatacttggc gggttattct gcctgttccg
     9061 gcttagtttt ctcaatgagg aaatcttcca tgaaatgccc ctcatcaagc agcttctgta
     9121 aagcaagcgg acgcttgcca ttaccagacc attctctgat ctcgccattt tcatccttaa
     9181 agcggtattt agcctttctt actccgcctt tggctttccg cctggttttt actgtatctg
     9241 ctgttacagg agcaaggagt gattcgggat caagacctaa agatatgatg tattcaatgg
     9301 cctgctgacg tttggcttca agttcctggc gttcagccgc ttcacgttca gcatcttcac
     9361 gacgttcttc aataacaacg ttgagctttt cctgcatttc gagcaactgc tcaaaatcaa
     9421 tctcacgggc aaaaacacgc aatgaacgaa tattcatcat agtacggctg ataatgccgt
     9481 attcatcttc tttagtcagt tcgctcatat aattaatcct cttatttagt gtacgtactt
     9541 ccagacgccg gaaggcacct tatcataaag tttattcatc accagttctg ctcggcgatg
     9601 atctgcggca gaataaaatg cctctgattc tgataaaggc agtttgtaac ggttaatctc
     9661 aataactttt tcaagagtat ctcttgatgt acattttctt aactgaaaaa gccattcctg
     9721 ctttgttttc acttcttagc ccttgccgga aaacccagct tccctgtttt cttatcctca
     9781 aggacaagaa ctgtatcata cgttccacca cttttctttt taaatccctt aatcaaatcg
     9841 gttttccctg atttaaccag tttttcggcc tgtgcctggg tgattttctt accagaaaac
     9901 tcactccaga ttttaaattc acatccggta cagaaataac ctttcggcct gataacaata
     9961 tgtttaccac aggaagggca gggagagtcc aggcgttgaa actgccctgc tggcgcagcg
    10021 ggttcaatct tcatctcgcc caggtcaaca tcagaaatca tgcctgtcag ttcaccgtac
    10081 agatcattaa taaactgttc aaccgtcagg tcgccatttt ctatggcagc ctgcttttca
    10141 gaccataatg ctgtcatatc aggattaacc gctataccag gcagggcatc aataagcgca
    10201 tatccggtat cagtaggaat aagtttccct ttttccagcg tgatatagtt tcttttcttc
    10261 agcgtttcca gaatggctgc gcgggtagct ggcgtaccaa tgccaccatg ttcatctttt
    10321 ttgtctttat ccttatcctt caacaatttt ttaattgttg gatcagtgac aaaatccgcg
    10381 acacgaacaa gcgcagcaag caaggaggct tcggtgaata acggcggcgg tgttgttttc
    10441 ttctcattaa caataacttc ttttgtcgtc agtgtttctc ctgtgcgaat tttacagagc
    10501 agttcaaaag cggaatcatc attatcttct gattcacctt cgtctgtggt ttccgcgcca
    10561 agaaacgcct caaatccgct gtcagttgtt tttctggcac gggcatagaa cgactcatca
    10621 ccacactgaa tggcaaccga tacttcctgg tatgcttttt caggcatgaa ctgaacaaga
    10681 tagtgttgtg cgatcgccaa gtaaacattg cgctcgtcgg tgctgagtgc gttaacatca
    10741 ggcacactgg cggtcgggat tatcgcagta tgcgcagtca cctttgcact gttaaacgct
    10801 ttgcttttac gtgcagaatc aatatccagc gactgaggaa agactgattt cagggcatcg
    10861 ataacctgcg gcgcttcgct gaattgttca tctgaaagat atgagcagtc agagcggtta
    10921 tacgtaattg ctttatattt ttcgcgtagt tgttgcgtaa tatccagcgt tttttgtgcc
    10981 gtcattttaa acttcttgtt catgtattgc tggagtctga ccagattaaa cggcaacggc
    11041 gcagccgttt ttttatcatc agttgctgct gcttcaactg tagccggttt tcctgcaagg
    11101 gatgcagccg ttccgtctgc ccacgcttta tcaagtaatt tacggtctgt cagcggagca
    11161 aattcccctg gcttccagtt cgccctgata acatcagcac cacgctgaaa gactccggtc
    11221 atggtgtagt aaaaactgga tttatggttc tggttagcac gggtacgatt cacaatcagg
    11281 ccaagaacgg gagtctggac gcgcccgaca gacagaacgc cctgatatcc tctggctttt
    11341 gccggaatgg tgtacgcacg tgtcatagag aagccataga cggcatcggc aactgaccgc
    11401 gccaacgcct taaggtaaag tcctttgaaa tcacgattgt ctttaagatt tgccagtgcc
    11461 tttttcactg ccggaagcgt gttgtcgtta atcagaacgc gctttacggg ttttgtgttg
    11521 cctgcatatt ccaggacttc atcaaccagt agctgtccct catcatcagg atcgccagcg
    11581 tgaacaattt cagtcacgtc tccacgtctg ataagttcga gaatcgtttt aacctgtttt
    11641 gctgcacttt caacaggctg atacttcacg ggataaagac gtaaaggaag cgtttcaacc
    11701 ttccaggctt tgtattcagg attatagttt tccggcggtt gtgattcgat aatatgacca
    11761 aaacagttag tcacaatggc gttatcactt tcaaaccagc catcatggcg ggtaaaattg
    11821 ccaccaagtg ccttaacaat atcatttgct actgcgggtt tttctgcgat aaaaagtctc
    11881 attttgttat cctttacatt gaattaatct tttggcacaa cggatagcgc gttcttccga
    11941 tactgtatgt tgccagttat ctttacttac tactctgaat ctgcgctcac agccattaaa
    12001 ggcaaaaatt ccctcacggt taagtatatt agttgcaata tcccactctt taattctcaa
    12061 aaaggctgtt acagcaaaac cagaatacga agcaattttt gaaatgatgc attcatcagc
    12121 ttttattaac gcggcaaaag gaactatctt ttgatgacca cacgaaatca gagggagtgt
    12181 gtgaatatca taataagcta ttgaatgaca atccatgcgg gtatcatcaa tagctgccag
    12241 ccagaatcct gcctggtctt ttatttttct gctaatatct gaatctgaaa caccagggtt
    12301 taataaatat ccaccataac agatatggaa ggggtgagca gattcatcaa tatcaggaag
    12361 ttcttttacc ggattttctt tccagtcctt cagaacaccg aattcaagag catccagcaa
    12421 aaactttcgt tcaaatccat caaacaatgc atattgcatg tgttaatcct ccaggtaact
    12481 taaatagtca ggttgtgccc cgttcttttt ataaagttct tccctttcct ctatatcttc
    12541 atctgcttcc tgaacgctca gatcgagggt tcctttcaaa aaggcacgag cattttttat
    12601 tacatccact tatgcactac cagcagataa gcacaataaa atagctataa tagatttggt
    12661 tactttcatt tctaacctca gtgattgtaa ctaatcatca catttcgttg gcgcgaaatg
    12721 tgatacttgc agtaccctga tacccgttgg cgcggttatc aggttttaat taacctctga
    12781 cacgaccaaa cttactcatg atttttgata tagcagcagg atcagccgtt gaacactgat
    12841 tcagaaatgc tttacgggca tcaaaagttt tactccagcg aatgcttcct tttttctttt
    12901 tcagaatatt aaaaaaatcc ttttcagcac ttctgcattc actccctcca ccttgcccga
    12961 ctgcttttcc atataagcaa agtaccgaag cgcaagggtc agatgcatta gcatttgttg
    13021 atattaatcc taacgccagc aaggctggta aaaccactct attttttcca gtcatttttc
    13081 acctcatttt gtgctttaat gaaaactatt tttacctgtt caggtaaaaa acttttctcg
    13141 tttcgccatt ccacgactgc tgttgcccct tccatcttca acagatctct gattcgaaac
    13201 cacagcccag catcatgtgt tgtcactaca atcctgtccg catgagccag agcgtaataa
    13261 aacgtcggcg ttctggcctg tactccggat aaggaagtga tctcataaac ccatccttta
    13321 ccgctaacgt ctgactttat gacaccgtga aaatcgggga catatggcaa tgagggattg
    13381 atcacttcat ttgccttttc aaactgcacg ggtgttggct ctggcggtga tgaacaacca
    13441 gcaagaatgg aaatcaataa aaacactaac ttcttcatca tgttctcctt tatgccgctt
    13501 tatcgagatc aggaacatct tttactacca gatacccttt tgcgacgatt tcattttcaa
    13561 atactttctg tggaggcata accagctttc ctttcttcct ggggaaaatg tttgcaagtt
    13621 ccggagcaac cttcacaaag tcaccaaaga aatactcatt gagatagtag aatgcctttt
    13681 cacaacgaat cgggttttct cccttcattg ccaggatttc ttcactaaat ttcagtgtaa
    13741 gtagttcatt tgttgttacc agtggacgct caataaggct ttctgattca gatctcgatg
    13801 ttcctccacg actacgactt ttgcttttcg aaccgatagt tactgttcgt gtccccatcg
    13861 cttctgataa ctcccgcgca tctttaacat cagaagcgga ataaacaatt ttgcagggtg
    13921 cacttgccag taatgtttta ctgcctgcat aactgtagat ttcctcaagc tgagagccga
    13981 cctggtaaac aatcaacaat ttcagcttgt aaccagcaat gagaccagat ttactcttga
    14041 tgtaatctac agcaccaatt gacgggaatt catccagtag aaaaaggcag tcatgcttta
    14101 tttccggatc atccgttggc atcttttcaa gattcacctg tatagcaagc tgaaagaaaa
    14161 tatttgttat tttttcagcc aacctggcat tatcaccaga aatacagaaa taaattgtca
    14221 tccgcttttt ccgcacctgc cgcaaatcaa aatcatttcc atcagtagca tttgccacat
    14281 tggggttagc aaacggtgat aatccattaa gaaagttgct tttgagttgt gcttttgttt
    14341 tatcctccat ctcaatgaat gccttaaggc cagcgacacc aatatcagca agcgttttgt
    14401 ctttccccct taattctgaa cgaatatcat tcatgaatga aagaagatgt tcgggttctg
    14461 cacgctcaaa aagctgatta attttagaca ttgagaagac aggctcaata tcaagcgttt
    14521 ttagtccagc cttatcattc ttaatcataa accataaagc gacagcataa gatttaaaaa
    14581 caccacccgc cgtcttatta agatagctac cagcatccgc accatctgtc ttataggggt
    14641 aaataatcaa cgaaagattt tcaagctgat taaaaccttc actcgttccc atgctgacat
    14701 aatacaaagg gttaaagcgg tgcgttaccg aactaaaggg atcaaattta taaacctcat
    14761 gacctaaaat caccttcctg ataagactgg tgatattaaa acactccctt tttggatcga
    14821 gaataataac actccaccag tacttcataa gattagtgat gactatacca gcacctttac
    14881 cagcccttgt tccggctccc atagcactga atgctgctcc cacatagcgt atgattttct
    14941 tgtcttttat accaacgata atcccgtctt tttcgtcacc gtcaagcgtg aaactgttag
    15001 actcacgaat atcattaatt gttgcaaatc gcgcatcacc gaaaatattt tctttcttct
    15061 cgttcagttt tatcaacgcc gcaacaggga tgataagaga cacgacaaat cctgctccaa
    15121 gtgcaacaaa ggctaaaact ctgatatccg tagtcacaac agataaagta aggctgtctg
    15181 taaaaatatc catcctgtac gcatcaatgg atctgaacac agccttcaat ggtttcagat
    15241 tctttatctg gctgaagtaa agcacaaaga aaataaatga acctgcaaac catgccgcaa
    15301 caagcataac agcgaatata aaaacagatt gcgacttatt aaaagttttc acattcagcc
    15361 cctttgtaat agatgtcgct catatgcctg acaacaccat ccctgcctac atgaacaaca
    15421 atatcaatac tgtccataat gattttacga agaacatcaa aaggaagatt ctgacattct
    15481 ctgttctgat aacattttgt caccatctga atgatggcat cttttgccga ggccgcgtga
    15541 atggacgtca tgcttccact gtgaccagaa ccagcaactt tcacaaaatc ccaggcatcg
    15601 ccacctttaa tttccgccag taaaattctg tcaggtttca tcctcaaaca ggatttgatt
    15661 aatttcgctg ctgttatgat agagccggaa tcactgccgg attccgaagg gtaaaaaaga
    15721 tgcacataat ttttatggat aaaaaatttg atttcctctg catcttcaat cgtaatcagc
    15781 cgtgtattca atggtatgaa atcgataaga ctcttcatat aagttgtttt accggaaccc
    15841 gttgctccgg caaatacaat ggttttccct gctgctacgg caagctccat aaaagcagcg
    15901 atatttctcg ctctgaataa atcaagaagc tcatcatcgt atgtcctgtg tttttttcct
    15961 gactggacat aatcataaaa accgttcgcc acatattctt tatgtgttat ctgccgtgtc
    16021 gatggtttac gtatagtgat tgaaatagta tttctctcac atgcaggagg aaaaacgacc
    16081 tgtacacgtt ctcctgattc cagtgttgct gaaagtaatg gcttggtctc gccgatttca
    16141 tctccccgaa atgatgccag cgtatgagaa aaacgtttac aaaaatcaaa ggtcacactg
    16201 tcattattat ggaatgtcca gtcaccatta atttccgtcc agatttcacc aggacggttg
    16261 acagcaattt ctgttagccc ttcaatatca agaaaagact ggaatacttg atttttataa
    16321 aaatcgagcg atttattgct aatgctgaca ggaataacgc cactcatact attttacctt
    16381 taactcatag atatcagaaa aatcaatgtc ttccccgacc ataatagtga ttatgtcacc
    16441 ctggttttta tacattgttg gaggaatatt tatcgaattc tcaagtgcga ctttagccat
    16501 ttcagccatt gcctgtcgtg agttttcggt ataatccaca ttacgatctt taccaggtgc
    16561 attatccgcc gccgccgccg ctacatcctg aaccatactg agcataagag aaccaccaaa
    16621 acgctcccag aagtgagaat cgatccatcc atcaatgccc gcttctccca ggggaccagc
    16681 tgctgctgta tttaccatat cgattcgttt attatcaggc gtccgcaact gctcccacat
    16741 aacgaacatt ctgccctgtc catgattcag cgttccggtc tggtaacgac caaaagcctt
    16801 agttccctgt tccagtaatt ttgtgtggtg attagcactc catacatctt ctgtaaaaac
    16861 acaactgata cgacctgcaa catcagaaac aaagcgtgtg gttaaagcac acggtataag
    16921 cctgttttcc tcaatataga gatcaggatc gagattaatc gcagttactt tcattcctga
    16981 tttaccaggc acattttctt ttgattcttt cctttcttcc tgtggctctg atgtctgcga
    17041 tgtcctgctg ctgccactcg cgccagtctg actccctccc tgaccagcaa caggtattga
    17101 caaatatttc ctgaacccct cctgtggcac ttctttatga ccgtcagaaa cagtattacc
    17161 agaaccagta gcgcctggat tgataattgc tttgttctca acagggtcaa aattctgacc
    17221 aagaccagta cggattttac ttacctgaga aatgctggta tcgcctgttg attctgatgc
    17281 tttctgctgg tcatctgcaa aataattttt atatgttttc agcgcaaaaa taccaataac
    17341 taatacagcc atcagaccaa acagaaagcc agaacgcata cgattatttt tgttcctgat
    17401 atcaccgata tcatgaacgg gtttactttc cagctttgac tgattcgcat catcaactaa
    17461 atcatctgtt tttttcatat cagtcattat tcacctcaac acgttctacc gcaggagaag
    17521 atgtattcct ctggtcggtt aatacttttc cgaaagattt attcacaaca ccaacaacgc
    17581 tgtttccata gcgtaaaaca aatttgtcat taacgttgtg gataaccata attttatact
    17641 tgccttttgt ttccacatta aaagcaagtg tctgttcctg accatctctt acagcaaatg
    17701 gagctgggaa tactttccct gagttaaaac ctaaaaaggt aaatatgcca ttatcataag
    17761 caaaatcagg ggcgatacga cgactgtcat atttttcatt gactcgcatg aaataattcc
    17821 agtttttagg cgcatcggct ttttcaaaac tttcagaaat cagtttcttc tgtctgagtt
    17881 tttcctgttt ttcttttgcc agccttactt cttctgcatt ctttttcgct atttctgatg
    17941 gatagcggaa ttgtacaaca taagcaggtt gcgcttgttt ttcttctgac agaaggatta
    18001 gatccagact gtaaatccgc tttgttgttc tgacaaaaag attcgtatcc cattcctttt
    18061 ctgttggctg gaaaaccttt ttcagttttt cgccttcatc gccttcctgt tcctgaacaa
    18121 cgggacgcgg attgatgtaa acaacattat catcggttgt gatttcccat ccggcaggaa
    18181 aaccagcctt tgcactgata acagcctcac cttcatcaaa aacaactgat gtgagaaatc
    18241 ctgtctttga ccggataacg gtcgtattcg caccgttata actcacagtc tgcatccttg
    18301 catcaaatcc agaaggtaac ggagtcgctg ctgaatacac aagcccagga agcaataaac
    18361 atgtagcaat aaataatttc ttcatcattc ccccctgact tcccgatctg tctggtaact
    18421 ggttacagta aaaccgagag gattaagctc acgactggaa cttttaaccg acttttctgg
    18481 ttcaaaacga tatgtcacac gagccgtcca gtatgaaact gaaacctgtc cggtcgtgaa
    18541 gtttctcgtc gtttttttaa agcgcacaga ggccagttta tctggcgcgg ttgcgtcaga
    18601 aataatattg gagatgacct caaccgcaac gtaagaaccg ttattatgat aaattttatc
    18661 aggagccata tcgctgttat acagacgaag ataatcgtcc tttactgtat tcgagctata
    18721 tagctgtaca gtctcataat catactgaag agacggataa ttataacctt cacgtaaagt
    18781 aatatacgtg tttacaaaat accgcccaag tgcttcagat tgcaggatct tttcctgttt
    18841 aacctgagta atcagttctg tacggcctgt tttttcgcca acagccacaa tagcaagctc
    18901 tttttctttt aatggcacaa gaacacaaat tgcagcccca agacataagg acagtaatga
    18961 tgctgccact gcaacacgcc atgcattttt cttagaccgt tcattttctg catagagttg
    19021 tctttcgaag gatttagctg tctcaatctt cttttcgatt ttttcactca taactgatta
    19081 cctggctgtg gttaatgggt tccagttcgc cggaaacatc agaaggaagg ttattccttg
    19141 tggtacatcc ggaaatctga aacaggaaaa acaaaagaat cacacatcgc ataaattcac
    19201 cttattaagc gttcacgaac acctttaaga gataaaggcc aagatgaaga aaaagaatcc
    19261 caccaataag aagcaaaaag cgaagatcca taccctttct ccatttcccc gcaagcccta
    19321 aaccaagtct tattcctgcg aagcaggtaa acactcctaa aagaaggatt aggacaaaat
    19381 aaagtatcat gacaaaataa tgaagcatgt cacaaccagc tctgactgat tccacctaag
    19441 accttaaata aggtaattcc cgtgattaaa ataacaagct gggttattag ctttctcttc
    19501 ctgctccatc cggaggtgat atgtttatca acgaacacca gtatccagtt cagggacatc
    19561 acataaaaaa aacgtttcat tattttcatg cggcgttcct cgctcgtgct ttttccaggg
    19621 atgcaagtct tctcgccttc atccccaaag cacctgtcgg agccagcgcc tgatatccct
    19681 tttcacccgc ccattcagcg gctgttcgcg caccacgccc catcagattc cctgctttcc
    19741 ctgataaaga attcatgccc gttcttccgg ccatccctaa gccaaaacct aaaccagcac
    19801 gaccgccaag catcagagac ttaccagtag ccataccagc agcacccagc cccatcatgg
    19861 ctgcgccctg aacagcacct tcaacacctg cacctgctaa ctggacagca aaacctttag
    19921 ctataagaac caataaagcc cccaaaaacc cagccataaa gcccatagca cctgtagata
    19981 caagatttac attccctgtt tgtgcagcac gaatagcatg ggaaaggatc atcccttgaa
    20041 aatccattgc tatccgaatc actaaactgg caaatagaac tgtcagaata ctggagaata
    20101 acgattgtaa ccaattatta aacatgctcc gaataaaacc aaacatgaga caaaagataa
    20161 atatcggtgc tgtaataatc aggagtttca tcgttacatc tgctgttaaa aaaacaacca
    20221 cactaacagc cattaaaata agcgagccag tccatattaa aaactgacca actacacctt
    20281 gatctttaac atatgttgat ttatctaaag agtatatttc cgcaccaaga ttttgcgtac
    20341 ttttccataa gttatctaaa gtttgccaga cactcacccc tccagaaaaa ccatctttca
    20401 tgccttgcaa cgcatcagta gccgcagtta aatacccatc cgcattagtt ataaacatga
    20461 ttatgattgc aaattttgat agatcccata ctaaatcctg caagggcgtc tgagttttac
    20521 tggcaagtat ttgatatcct ttccatagta catataacgt aaccgatacc gttgctaacg
    20581 acataatatc attagcaaat tgtgtttgat aactggagct gattttatct accgaatcca
    20641 taacggtaga attatattta acgaaaaacc cctgagccat atatccacct tttaatcaaa
    20701 agttaatggt tcagcttctt tctgctgttt aataaaggcc tcttgttctt gtgtgaccaa
    20761 catctcatga tctgctttat ttttattatt ccagacatca tactgcattt ttatcgcctg
    20821 catttttaac gactgcgcct gtaatgcgtt agccaaatcc tgggattctt tgatatcctt
    20881 tgaagcctca attcggctac ttaaactctg aatctgagac attgcacttg ataactgctt
    20941 attgatctca tttccctgtt caatactggc agccgttgac acaatccgtg ctttacagag
    21001 attatcatta cgttcatagc ccgtattaca catatcgaag gctccatact tgctgaacag
    21061 atttttagcc tggctgctca atgcaccttc gggattactg gtaaaatcag aaatataact
    21121 gttcccctgc ttataaatat ttgtcagttc tgattgtaga ttacttagtt catttgtgaa
    21181 tgccgcaata ttccttatac ctgttgccgt ggctaactgg tctttatatg cctgaatctg
    21241 actctgataa tgttgcgcag tttcggacca ttgtttgagt cgttctgtcc atcttgcggc
    21301 ttcaacagcc cattccggac tggcatcaat agcaaccgga attcctgcgc tactataaaa
    21361 tgaagccgtc aataatgata tcagcatcaa tgtttttaca cgtttcataa attcacctgt
    21421 cagattgccc gttcgagata ttcaggaacc cagtcatcag gctccattcc ttcatgataa
    21481 atactttcaa aaatttccag attatctgca ctggcagaca atattttcag atatccacca
    21541 agtcccgata aatcgagtgt cgcaagtgct gaaaatgatt taccatcccc ctttttaaga
    21601 ctgctttttt taatcaggaa ctgtctcgac attggatcaa gatttttaac aatattaaat
    21661 tcttctggtg tcagcttaag cccttccaca taatcattgt aatcggcatc agggttagcc
    21721 agataaatac tggtgctgca tacttcaacc acagcccgtg aaatcttatt cttcagtatt
    21781 tcatccggag actgagttgc cggaataacc agcccgttca gcttacgaat tgttttcagc
    21841 ttgttataga caaagtcgct gaatgcttca tcctgtagcc atttccagaa ctcatccagg
    21901 aagataacaa gacgccgacc gtccagaagc tgcgtaatgc gatacagcag atagaaagta
    21961 attggtgcac ataccatcgg atcatcaaga aactcggtgc catcaatgcc aaaattattg
    22021 acatgctgaa tattgaatgt gtctttagca ttatcaaaaa cccagccatg agcctgacca
    22081 ttagcccatt gggacaaacg aaggataatc ccgttctcct gctcatcccg atcatctctc
    22141 tgcataagat gctcaagcat tctggtaatg ccatattccc gcatttcgcc aggaggaaaa
    22201 tccatcacag catcaacact ttcgctaata agcaatcttt ctcttgtcga taatctttca
    22261 ccatttctgg tacacagaat ctccatcagt tgcttgacaa aaatacggtt acgttttgtt
    22321 gcctccagag caaaaggatt ccatcctgtc ggctcaccgc tttttacggt gtaatattcc
    22381 ccaccaagca ttcggataca taattcagcc cctctgtcct tatcaagaaa gacacacgtt
    22441 aacttcttat tttttgcaga atcagcaaaa gtttcggggt tattgtattt ctgtaatgaa
    22501 caggcaagat atgacagaaa cattgtctta cctgaacccg ccgttcctat aactttagta
    22561 ttagcaagat tcttttcacc tgtttcatct ttaaacagta ctgagttatg taaattcaga
    22621 taatacgcct gtttgcttgg tgttttgaga atagcaaccg cttctgtcca gcagttttta
    22681 tcgcgtttcc cctggtagaa attatggaat gatgccagtt caacaaaatt tacattcgat
    22741 accggagata aacgcggcct taaatgaaaa accgccggaa gttgggcaaa atatgccgct
    22801 ggcaatgaca tggttgacaa tgccggaata atgcccaggt cagtaaagtc tgccgtaatt
    22861 ttacttacag attcgtcaag ctccctgata ctgtccgcca ttaccattag cgtgaaatgg
    22921 tagtaaccaa aggatatatc accggacaca atatcatttt ttgccttttc cagttcctgc
    22981 aattgagtaa ccgcatcatc ttctgtactc tttaactgtt tttccgcacg tttaatggta
    23041 ctcaatgctt ctgatttgct catggatgta tatgaatgcg taataatata gtcagcatca
    23101 gaatactgga gcacatcaaa aacaccggat gcagtctcac tacagaaatc tttaatttca
    23161 atagaacgaa agaatttatt tccatcaatg cggcatatct gtccggtatc atgattaaaa
    23221 aatacatcca ctccaccaag aacgttatac accggagaat cagtgactct gattttttgc
    23281 aacttccctg tcagaagata attatagaat gacagttgag aagaaaatac ctttccgttc
    23341 tcctcataca taccaagaag tttacaggta aatttatcaa gagcgccaga gaatgtatta
    23401 atcatttcat tcattctctt cacatgaatg ttaatatcat ctttcttttc tttaatactt
    23461 tttttgcgtt tttccagttt ttctacacgc ccatctggtc tgtagatcag cgtaaagtaa
    23521 agagtgttcc ccttgaattt atttttcttc atcgaaccat agtaacaatc tgatattata
    23581 tcagcatatt tattacctga ttttgtggta aaggcatcat aaaaactcgc cctgcatgaa
    23641 tggttataaa atgaaacggg cagtccggaa aatgaacgaa caagagtggc aacctgtgag
    23701 tcaatagttt ctaacatctc cgcagagatt gactcaaagt taatccccat cagttgccat
    23761 gtcgctacat aatcaccgtt ctttgttttg acaatatttt tatcaacatg gcttgaatag
    23821 ggtatatatt taccagtagt gattcgctca ttcagtttca tggcattaac aaactcatca
    23881 atttcaacag cttcatattg tccggagagg aacgctcgtg cgccaaaaaa acgattacac
    23941 actgagtttc cgagcatttt cagcttaaga aagtaaaggt taaaaatgtg gtcgtcctgt
    24001 ttagtgatca ttctgagaac aaaaaccgcg acaggaatag ccagccagat cagcttgctg
    24061 atataaaccc ccgccagaaa taacgcccct gcaaccacag taagagggac gacaggaaca
    24121 cccacataca tagcagggcg tgtcatcgct ttataaagtg tagacatact atcgtccccg
    24181 ctttattagt taaccatcat tgatgcaact tcagccgcac tggcgataac aattgccccg
    24241 atgatgatag gtgagcattc acgaatcgtg cttccaccaa acagaacctt gtagccgacc
    24301 cacagacagg caatagtcac cgtgaccagt gaaaggccac taagccctgt tttaaccttt
    24361 tccagcagtg tttccgcttt tgaaaatcca tccgcaaagg ccggtgatgc aactgttgcc
    24421 ccggcgactg ccataactac cttattttta cgctgcatta cagcagtgac agatgaactg
    24481 atacgattac atactgattt cttaaatgac attttataag tcctgttaat taataacttt
    24541 tgttggatgt gagttatcaa caaaagcaga tttcagaatg gtttcgggcc agataactga
    24601 cgcattgtgt gaacgatttt ttcgctgttc tcccccgttg cttcttgtac ctggtacaac
    24661 atattttttt tcgttccctg tataaccaat tcgctcaaca taacttgtat tattaaattc
    24721 tttttctttt cgcttaccgt tactaaaatt tcctgtatag taacaactaa gcgcattctc
    24781 caggctcctc cctcgtttat aacaatccac gataatcttt tcgaaaacag acaagttttc
    24841 acacgaatca aggagttttt ctgcggacgt ggaataaaaa gaaaaattac tgctggtaat
    24901 ctgcatcaat ccgacagaat aacgtctttt tctgttttca atttctctga taactgttag
    24961 agcttcctcc ttacttttgg gcatatgact gataacactt ttgccatagc taccgcgttc
    25021 cctttcagga ataatttcag ccacagcata aggattgaag ccactctcga cccgtgcaat
    25081 atcatttatc gtatccggat gaactgacgc cgcacacagc aacaaggctg ggaataaaga
    25141 cgccatagct aacgctccat atatccatca atacgcatac tctgcattaa taagtaaata
    25201 atttgcttcc tcttactaag caaagatgtt ttcgttttct cttcgataaa tgcattcaac
    25261 ctgggatcga gttttatcgt gacataaacc cctttttccg atgacttatc tgatttctcc
    25321 ttcatttaat caatccacta taagtgtgtt ttggatttgt tttagatgcg aacatgattc
    25381 gcaaaataac aatgctcaac aatacgatca aataacattg acaaaatggt catattatga
    25441 ttcgtgtgat catgcaaaaa aaagcgccca tatgggcgct ctagtatgat tgaaacatta
    25501 gttaatcgaa tgtagggact ggagcatcct tctgagactg aataaacaat gcttgtttaa
    25561 cattcttaca gttctgagaa tcagagccag acttctcaca ctctgcctgc ttttgtttag
    25621 cttccgtaag atgctgcccc caccaatcca cactttttac ttcttcacta caaccaacaa
    25681 cgaagaacgg caaagcaaaa agcagatatt ttttcataaa ttccccctct cactgatcct
    25741 ggaaagactt gacacgagaa gggcattagt ccgaatgagt accagtgttc tgatccctgc
    25801 caacgagaca cctcccagtt cagctcttac agaatcgaga tattttctga cagttgaagg
    25861 ctttagtcct ttgaaatcag caatttcatt cagggtatag ccataagcgt acatcagaat
    25921 aatgtctttt tgttgctcac tcaaatgttc ttcatcacta aaaatttgat gaagtccaga
    25981 aatgcagata gcatcatgct cagtcatgat aacctccctc gtaggttgtt gtgatcagca
    26041 ggtgagcaaa gtagccgctt tgttcacctg cgccaaaatc aaattaaaat cccaggcctt
    26101 tccagttcct ctcttgctgt attcttagtt ttttatgttc ttctgaatcc tttgtaaacc
    26161 cgtgttcaag agcaaacccg atattgtgac cttgctcatg ttttctggat agctgctttt
    26221 taacgtcgct ctcttccggc tgcctggttt cacgctcatg aacctgacgt tctctgttcc
    26281 gctcaagggc aatattttca atctgccagt tattgcgatg cgtttccctg tagcctgcaa
    26341 cagttccatc atcattaaac attggactac gtacaagcgt aggagccagt ttttttactt
    26401 tgataagcgt tcctggctgg agcttttcag actcgaaatg atttttcagg tctttacccc
    26461 aaatcgtaac ctgttttcca ccttttaaag tttcataagt gaggaaaggt gttcgttttt
    26521 cccctgcctt attctgatat gaagtctccc caaaatccac caccttgtaa acattccgga
    26581 gtcttttcgg attttcagcc tgtaatcgct caatctcccg tttacgttcg ggatcttttt
    26641 tccaggtagc ggtaacatca tatcccatac ctttcagttt tctatgaaaa ccgtttctga
    26701 acttcctggt ttcagattta gtaagtctta atcttcttcc tgtttcttca tcacgaagtt
    26761 taatattaac atgtacatga ggatgatctt ctttatcatc atggtatacg gcaacgaaag
    26821 catggtttgg ataagtctct ttaagaaatt ctaccgtagc tttcagagcg tcttccctgc
    26881 ttactccagc ttctggtggt ggtgaaaaga ccatgttttt cacatgatcg atgttttctc
    26941 cacgatatgt cctggagtaa tcatttcctt tagataaagt tgaagtaaac tccctattaa
    27001 agtcttctcc tttccctgtt tgctctgaac cttttccatc ataacagtaa gcctcaagtt
    27061 caccattacg ggtaatgtaa tcaataccat ttttgatgcc tgcggctgtt ttacctgagc
    27121 cagtgatttt aaaagtgact tcttttgtag gtgtccaggt atatgctttc ttgtccgcac
    27181 catgtctgac tttatgatta aaagccgatt tactggcatg tatatgtctt cccgataact
    27241 taggagattt cggatcacgc ccagtccctt tagcgcgttt aatacggtat tcctgttcta
    27301 catacacacc cataaatcac ccaaatctgt taacaacact cttggttaac gtttcaagtt
    27361 ttgaatcaat ttgcttacac agttcaatca cttcaataac gtcttttctg aaatctggat
    27421 cgttaaccgt acagaatctt tcacctttta tgataaaatg aatgttgcga ccaacacgat
    27481 ctacagcagt tgatagtttt ttaagttcct taacttcatc agggtaaaga cagacttcac
    27541 tctttaacaa agactgacgc acacgaatag caatttctct gctcatgctc atgccgtgaa
    27601 gagcagcatt acgtttaaca agttctattt catgagggta aagacgcact gataccagct
    27661 ttgctggttc attattaact gggatgttaa caggcttggt ttcaatatta agcacgttat
    27721 gcttagcaaa agcattgata ctggtaaatc cacttaaaat agcgttatgt tctgcaaatt
    27781 ttaactcatc atcacttagc ctgaacgcta tcatcttcat aagtcacccc aacagagcct
    27841 gcggcaggca caggcaaaaa catcgttttt ggacaatctg attcaaatgt taacatttga
    27901 catcggattg tctatcctgc attgattgta ccatatgtgg cactgtgcct ggctataaaa
    27961 tcgtcgctgc atatgtccag atttcgttgc tgaaagattc tttcgtcttc gaccatcagg
    28021 caaacgtata gctgaaagat ttgtgaaaga aaaaacaggc ctggcagggc tgggaattgt
    28081 tacggctgaa gccgtaacgc tttttaagtc ctcgctgcgc tgcggggctt ccggcgcaaa
    28141 gcgcctccca tgcccttaca gggcataaaa acaagggcaa ttaagcccct gcgcgatgat
    28201 tgctgacttc tttttccaga gccttattaa caaaggcatt caacgataaa tcttcttcca
    28261 tcgccatttc tgctacccga cgatgcagtt ctggatcaag cctgacgtta aacacgcctt
    28321 taaacggggt atcaggttcc tttccatcct ccacacaaga ctgtaaatac agatcaacag
    28381 atgttttaaa ctcctgttcc agttcagcta atgtagaagc ctcataagtt accaggtccc
    28441 gaataaacgc cagttttcca tacaggacat tattttcaaa atcgggttca accgtgccta
    28501 aatatccttt atattttaaa tgtttcataa taatccagcc tctttcagat tctgtttaat
    28561 tgctttcagc gtaccacctt tgatataact ttccggatgt ggacgatgca ttaatatggt
    28621 gtgattgatt tcagcattga aaaaccgcac tcttgacccc tgcatttcct ttttgacata
    28681 tcccaaagag gaaaacaaaa cgaccagttc atcccattca aacgtttttt tactgtttaa
    28741 aaactttgcc agtagcttat ctgttttccc cataaatcca tcccctatag atatgcaact
    28801 aattatagtt acacatggca tttttgtcaa aaaatacaaa ggggcagaaa gccccttttt
    28861 ttatttaaaa catttcatac ccttgcaagt agtttaacca ccaccggaat tgatcccatt
    28921 cgctaaattt cattgccgga attctggttt cccagccttt gacaagggaa tcagcaagct
    28981 gtacgatctg ccgcgttgac atttaaaaac ctcattcagt tttgattgtc gataacgtga
    29041 tcaggttggt ggaacaacct gatcacacct tgaatagtca gtcattaccc tccttctttt
    29101 ctatagttac tgtgtatccg taaagagtca aaatgcgata agcatccaac gcctcacgct
    29161 cacttttaaa tgttctgact tcgtgtaatc ctttaccttt atcaagggat acacaaaact
    29221 cataatcttc ttctacttct atctttttaa ttaatttatc aagttcggct aatgttcgtt
    29281 ttggctcaaa aagaaatgtg cattgtacaa tggcgtttaa atcttcccga tcaagctcta
    29341 acagagcatc attataagca gctttaaaac agcgattatt acgcagatca gatgcaaact
    29401 gcattgcggc ttgttttaac gttttcatga atttcccttg cattgatgtt aatagacaat
    29461 ttcagaatca atattgaaat ttccagaaaa tgcagggatg cccctgcatt cataatgctt
    29521 aacaaagaca ctcaataaaa tctttaaaac tcaagcattc atcgccatct tttaatgaat
    29581 caaaatattt ttcatattcg tccctgtaga aaaggactaa atcaacatca ctctgcttaa
    29641 gttcgccaaa ctgaggaaca attccggctt tatagtcacg aattttattg ttatcagtaa
    29701 atatgacaac atactgttgt ccagtatcct tatgcataag gataatagtg caaagcgtta
    29761 ctcgttcgaa tgtgaaaaca tgattagtgg ttgaagtaaa aacagatgag catttcattt
    29821 taaatctcct gccctgctgg gctattgtga gaggggccat tcccctttcg atgaaacaat
    29881 aatacgacct ttaagtcgta tttgcaagtt ttctcccgtt tttttactcg ataagtgatt
    29941 gttatcacgt tttttaatcc gcgtaagcgg atggaagggg cgaacgaaaa taccagggca
    30001 gcacaggagc cacatcgccc cgaagcgcgg cgcagccgct gacgaacgta ccagggtgca
    30061 caggccatcc cttcaccctg caaagcccaa ctgaccagca ggccatgatt atgctgattt
    30121 gcgccgcaga atgccgcagt tagcgcgagc gcaagcgagt ttaccccgcc ctggtgcatt
    30181 gcagcatggc ggggcgctgt actgcgcgtg tgagcgtcag cgagctacga gcgcaactcc
    30241 aggtactaat ataaaaccat cacaggtcat tttattttga ctcccttttg cagagcacgt
    30301 attcaggatg ttctcctgca agtaacaaaa gaaactcaaa ctcaaccagt gacagacttt
    30361 gactgttttg agtattaact tcctcttttt tctgccaggt tcttagccca acaccataaa
    30421 tatgactgca ttccttttgt gttaatcctg cttttaaacg taattttttt acattttcag
    30481 gtgtattatc gggtttcatt taacataaac tccttaatta aattcttaac tggcgtctca
    30541 tcgtcttccc ttaatccggc acacgtaaaa tcaatatgtt gtaattcatt ctcattcagt
    30601 acagcatcat acaatgtaat tttcgtagtt ttgttcccga ttttaacatt tgttctggct
    30661 tttatccttt ttgtaagcct gtaattgtca ccttcttttt caagaaaata agtcacatca
    30721 cctttatttt ttctcataac tgtttcaaat accctgtaat tttaacaagt tgatctatac
    30781 tcaagccccg aatctgtaaa tcataactgt cgccctgttc ggtgacttta ccgttcaatc
    30841 cgttttcagc tttaaagcct gtcaatgccc cataccagct ttcaagctgg acaagttgcg
    30901 ataacatacc tttcaggatc agaatttcgt cctttttttc cacctggtgt ttctcatact
    30961 caatcgcctg taacacctcc tgcgaagcat ttaccagaat ttttcgctcg atatcggctg
    31021 caatatcaac gggattccgt tttctggata acgtgcaaac atgatgcgga ctacgccagg
    31081 aacgactatc cacactcccc accacagaaa agcggtcttt ttccatccga atatgcacag
    31141 agtaattttt aaactgcggt gacgtcagtt ttatacgaga cgaacaatca tcaagtttgt
    31201 tcacgcgcca gccgttcccc aggatacggc agacaacttc aaagacaggg tgatattgtt
    31261 caaaaaaaga gttcatctgt ttcccttttt gctacgtggc tgctgttccc gtctttttgg
    31321 gttcgatgcc ccaaaagacg ggctggctat aatatcgggg gcttctcgcc cccgttctgg
    31381 ttacgctctg gtttccacgc gttgccaccc tgtcgccctg cgctgtccgg ttgtaccgta
    31441 ttttgcgcgg atttcttcta tcgtgtgttc ttcacgattc cagtaacttt tatgttcgtc
    31501 tggacgataa aaccattgtt ttttctttgc cgcccattta cacccgattt cttttaaagt
    31561 ttccttatgt gtaatggttt ctccactaat ccagacccag ttacctatca cttcaaatac
    31621 cagaccagat aaaccagaaa gaacgtttaa tactttttcc aggtcatcac cgtaattata
    31681 tcgtgcatgt tcatcagtgc tctggaactg atttatttta tcaatatttg ccatcaatac
    31741 atcaaaagct gcatttaccg ctttcatcag ttcagcccct aacgggttac gatctggatg
    31801 atattttaaa gcggcttttc tgtatgctgc tttgatatct ttttcagtta attcgccgga
    31861 taatccaaaa acgtttaatg cttcctggat attcattttt ttaatctcct gccctgctgg
    31921 gctattgtga gaggggccat tcccctttcg atgaaacaat aatacgacct ttaagtcgta
    31981 tttgcaagtt ttctcccgtt tttttactcg ataagtgatt gttatcacgt tttttaatcc
    32041 gcgtaagcgg atggaagggg cgaacgaaaa taccagggca gcacaggagc cacatcgccc
    32101 cgaagcgcgg cgcagccgct gacgaacgta ccagggtgca caggccatcc cttcaccctg
    32161 caaagcccaa ctgaccagca ggccatgatt atgctgattt gcgccgcaga atgccgcagt
    32221 tagcgcgagc gcaagcgagt ttaccccgcc ctggtgcatt gcagcatggc ggggcgctgt
    32281 actgcgcgtg tgagcgttag cgaactacgg gcgcaatgca ggagaactaa cagaacctcc
    32341 ctaaaacgct ctgaatcgca ttttaagcga tttttttcat ttcgcatggt tttcctcatg
    32401 gttgtatcga acgaagcggc agagcgcgat tcagggcttc ttagtggaag atccaaaaag
    32461 gatcaaataa cggatcatat gatctgaatc tctctggttt tgagaaagga gataccgctc
    32521 gccgcagccg aacggcagga gcgtagcgac tgagtgagcg aggaagcgga atttttgcga
    32581 tgtgacatta ggatcagtac gcacgaatat tatatttatt tcaataagtt aaagttaact
    32641 agggacaaat ttatggtctt gaaaattgag atacataagg catcaaatat ttttttgatg
    32701 attttatgtt attgaaagtt atgagtttta atgattgatg atgtttgttt tagcttgcgg
    32761 gtaataatca tcgggggaca gatttatggt aaggaaccat catcgttgct ggttttgttg
    32821 agttatccac agttcctgaa tattcgcacg cattaaaagc cttatctttt cttgtttttc
    32881 ttaaaaactt aaaaacttag agtagctcta atttattgat tttaagcgtg ttttttgtct
    32941 aaacatgaga gcttcctcgg ttaaacatga taacttcctc ggttaaacat gagagcttcc
    33001 tcggttaaac atgagagctt cctcggttaa acatgagagc ttcctcggtt aaacatgaga
    33061 gcttcctcgg tgatatccac aaaatgtgtc ataagaatac taattcgttg ttctttctgg
    33121 tgaatctatt ttgaatctgt tgttaatctg ccttgaatcc agtgtggatt ttgagcatgg
    33181 attacctttg tgagataaat cacagatatg aaacatgaga agatactcgg ttaaacatga
    33241 tattgattgt gttgtaatat catgtttctg gtgttaaagt ctgattatca agagtgttgg
    33301 aaca
//
