LOCUS       OY856305                6645 bp    DNA     circular BCT 04-DEC-2023
DEFINITION  Escherichia coli strain EC29472022BGR genome assembly, plasmid:
            P_EC29472022_2.
ACCESSION   OY856305
VERSION     OY856305.1
DBLINK      BioProject: PRJEB70793
            BioSample: SAMEA114783439
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ivanov,I., Teneva,D. and Stoikov,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2023) NCIPD, CMAR, Yanko Sakazov 26 Sofia,
            Bulgaria
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC29472022BGR"
                     /isolate="EC29472022"
                     /isolation_source="human urine"
                     /db_xref="taxon:562"
                     /plasmid="P_EC29472022_2"
     gene            <3..1511
                     /gene="mbeA"
                     /locus_tag="ECO2947_28860"
     CDS             <3..1511
                     /gene="mbeA"
                     /locus_tag="ECO2947_28860"
                     /EC_number="5.6.2.1"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniRef:UniRef90_P13658"
                     /note="SO:0001217; UniRef:UniRef50_P13658;
                     UniRef:UniRef90_P13658"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase MbeA"
                     /protein_id="CAK5471229.1"
                     /translation="PVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF
                     AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKR
                     LQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGI
                     DALYHAGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFADG
                     RGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRRYSPVHSLDRGIAGKTP
                     GRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRNQEAGR
                     DIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAE
                     RLRAAATGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLE
                     PVIQRELEIREERLIQEREHVLSLERERQPEIQERTLDGPSLGW"
                     /name="mbeA"
     gene            complement(1722..2141)
                     /locus_tag="ECO2947_28865"
     CDS             complement(1722..2141)
                     /locus_tag="ECO2947_28865"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_001254967.1"
                     /note="RefSeq:WP_001254967.1; SO:0001217;
                     UniParc:UPI00000B019C; UniRef:UniRef100_Q51623;
                     UniRef:UniRef50_F4T8P0; UniRef:UniRef90_Q51623"
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="CAK5471231.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2222..2659)
                     /locus_tag="ECO2947_28870"
     CDS             complement(2222..2659)
                     /locus_tag="ECO2947_28870"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_001405929.1"
                     /note="RefSeq:WP_001405929.1; SO:0001217;
                     UniParc:UPI00025CB33B; UniRef:UniRef100_A0A2P9E2X6;
                     UniRef:UniRef50_Q51610; UniRef:UniRef90_A0A5B9SUN1"
                     /codon_start=1
                     /transl_table=11
                     /product="Entry exclusion protein 1"
                     /protein_id="CAK5471233.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="Entry exclusion protein 1"
     gene            2919..4487
                     /gene="cea"
                     /locus_tag="ECO2947_28875"
     CDS             2919..4487
                     /gene="cea"
                     /locus_tag="ECO2947_28875"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_000447006.1"
                     /note="BlastRules:WP_000447006; RefSeq:WP_000447006.1;
                     SO:0001217; UniParc:UPI00000BB5A2; UniRef:UniRef100_Q51626;
                     UniRef:UniRef50_P02978; UniRef:UniRef90_P02978"
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="CAK5471235.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(4484..4825)
                     /gene="imm"
                     /locus_tag="ECO2947_28880"
     CDS             complement(4484..4825)
                     /gene="imm"
                     /locus_tag="ECO2947_28880"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_000058760.1"
                     /note="RefSeq:WP_000058760.1; SO:0001217;
                     UniParc:UPI00000032F4; UniRef:UniRef100_P02985;
                     UniRef:UniRef50_P02985; UniRef:UniRef90_P02985"
                     /codon_start=1
                     /transl_table=11
                     /product="Colicin-E1 immunity protein"
                     /protein_id="CAK5471237.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="imm"
     gene            4873..5010
                     /locus_tag="ECO2947_28885"
     CDS             4873..5010
                     /locus_tag="ECO2947_28885"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_001405930.1"
                     /note="RefSeq:WP_001405930.1; SO:0001217;
                     UniParc:UPI00000032F5; UniRef:UniRef100_P05821;
                     UniRef:UniRef90_P05821"
                     /codon_start=1
                     /transl_table=11
                     /product="Lysis protein for colicin E1"
                     /protein_id="CAK5471238.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="Lysis protein for colicin E1"
     rep_origin      5006..6036
                     /inference="similar to DNA sequence"
                     /note="origin of replication"
     gene            complement(5070..5174)
                     /gene="RNAI"
                     /locus_tag="ECO2947_28890"
     ncRNA           complement(5070..5174)
                     /ncRNA_class="other"
                     /gene="RNAI"
                     /locus_tag="ECO2947_28890"
                     /product="RNAI"
                     /inference="profile:Rfam:RF00106"
                     /note="SO:0000655"
     oriT            5864..5952
                     /inference="similar to DNA sequence"
                     /note="origin of transfer"
     gene            complement(6037..6228)
                     /gene="rop"
                     /locus_tag="ECO2947_28895"
     CDS             complement(6037..6228)
                     /gene="rop"
                     /locus_tag="ECO2947_28895"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_000165985.1"
                     /note="RefSeq:WP_000165985.1; SO:0001217;
                     UniParc:UPI00000000E5; UniRef:UniRef100_P03051;
                     UniRef:UniRef50_P03051; UniRef:UniRef90_P03051"
                     /codon_start=1
                     /transl_table=11
                     /product="Regulatory protein rop"
                     /protein_id="CAK5471239.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="rop"
     gene            6277..6543
                     /gene="mobC"
                     /locus_tag="ECO2947_28900"
     CDS             6277..6543
                     /gene="mobC"
                     /locus_tag="ECO2947_28900"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA
                     sequence:UniRef:UniRef90_UPI001F359810"
                     /note="SO:0001217; UniRef:UniRef50_A0A1X0Y5G7;
                     UniRef:UniRef90_UPI001F359810"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="CAK5471240.1"
                     /translation="MKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSG
                     KLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVTH"
                     /name="mobC"
     Typing          5865..5952
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(5296..5851)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.699"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4..1508
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.428"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 gtccggttga ttacctgctg gggagggagc gtaaccgcga aggcgcaacg gtgcttcagg
       61 gtaatccgga agaagtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
      121 ccggtgttct gtcgttcgcg gagaaggagc tgccgccggg aggacgtgaa aaagtgatgg
      181 cgagctttga gcgtgtactg atgcccggtc tcgaaaaaaa tcagtacagc atcctgtggg
      241 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
      301 agaccggaaa acgcctccag ccgtactacg accgcgcaga caggcctaga attgatgcct
      361 ggcagacgct ggtgaatcac cattacgggc tgcatgaccc gaacgccccg gagaaccgca
      421 ggacgctgac actccctgat aacctgcctg aaacgaaaca ggcgcttgct gagggcgtca
      481 cgcgaggtat agatgcactt taccatgccg gagagataaa aggccgtcag gatgtgattc
      541 aggcgctcac tgaggcgggg ctggaagtgg tcagggtgac gcgaagcagt atcagcattg
      601 cagatccgaa cggcgggaag aatatcaggc tgaaaggagc attttatgag caatcttttg
      661 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg
      721 ctgaacaacg agttcaggag gctcggcgaa tctgtaagcg aggctgtgac atcaaacgag
      781 acgaaaatca gagacgctat agccctgttc acagcctcga cagaggaatc gctggaaaaa
      841 caccgggaag gggtgaaaga ggcgatgatg cagcacagga gggacgtgtt aaagctggca
      901 gggaatacgg gcatgatgtt actggggata gtctttctcc tgtttaccgc gagtggcggg
      961 acgctctggt atcttggagg gaggatacag gcgaacctgg aagaaatcag gaagcaggaa
     1021 gagacattgc agaaactgaa cgcgaagaca tggggcgtgg agtttgtgca ggacgggaac
     1081 aggaaattcc ttgtccttcc gtacgggaaa tcagcggagg tgattccctt tcaggggaaa
     1141 gagtgggtac atctgaagga gtgacacaga gtgacagagc tggaaacaca tttgctgaac
     1201 gccttagagc agctgcaaca ggactatatg cagcggctga gcgaatggga gagcgccttc
     1261 gtggaattgc agaagatgtt ttcgcttacg caacgggaca acgcgatgct gaacgagcgg
     1321 gtcatgcagt tgagtcagca ggtgcagcac ttgagcgagc agacagaacg cttgagccag
     1381 ttatacagcg agaactggag ataagagagg aacggctgat acaggagcgc gaacatgtgt
     1441 tatccctgga acgggagcgt cagccggaaa tacaggaacg cacgctggat ggcccttcgc
     1501 tgggatggtg aaaccatgaa aaatggcagc ttcagtggat taagtggggg taatgtggcc
     1561 tgtaccctct ggttgcatag gtattcatac ggttaaaatt tatcaggcgc gatcgcggca
     1621 gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc gctgaacgcg
     1681 ttttagcggt gcgtacaatt aagggattat ggtaaatcca cttactgtct gccctcgtag
     1741 ccatcgagat aaaccgcaga aatcgtgtca gccagcagcc tggatttttt cggggtaagg
     1801 tctttgctgc ggtaatcagt acccgcaaat tgagttgtgc cccggagtga gtttaaaaat
     1861 tcctggctgt gaaactgctg aatgcgttgt tgcacatcac tccgtgattt tcctgtcgct
     1921 ttatctttag ctcccatatc ccagaactgc cgaaaaaaag gcaccatcat tcgtgttgtg
     1981 tcggcttttt gggtggaaaa gttgggatca aaaccgtcat cagaagcata tacgtaatgc
     2041 cgggtatgtt gttccacgct gccctttggg gtacaggctg tgagagctac tgttgctgtt
     2101 atcatggcga taagacaaag tttgtttccg gtacgtctca tgagtgtctc cctaccttaa
     2161 agtatttatt ctcggtgagt tattatccga agactatttt atcatcagtc tctgacagcg
     2221 ttcagcgacc gaacaacctg gaccagaatc cccgtttctt ttccagttcc agtgcctgcc
     2281 tgagctgggc tatctcattt tgtagctgtt cccgttctgc ttcctggcgt ctgcgatcca
     2341 tatcctgtgc ctgtttatcc tcaagcatca gcgtcaggca ttgtttcagc tcattcagct
     2401 cccggagaat gcgttctgtc tgctgatcat gtggattttc tgcatgtccc tcactgtgcc
     2461 tttctggtgt ctcattctgc tttaattcgc cgtatgcccg gatcagttca ctggtttcaa
     2521 actcccgtcg tccgtccctg ccggtacggt aactgacaag gcctgcgcgc atatcacggt
     2581 aaaactgtga acgcgatctg cctgtcattt ttagtgcgtc ccggagtgtg tgccatgcca
     2641 taaagtgaca gtgtcccata gatgtctcat ctcatagttt cagtaaaaca taatgaggtc
     2701 tgagaacggt aatgtttgtg ctggtttttg tggcatcggg cgagaatagc gcgtggtgtg
     2761 aaagactgtt tttttgatcg ttttcacaaa aatggaagtc cacagtcttg acagggaaaa
     2821 tgcagcggcg tagcttttat gctgtatata aaaccagtgg ttatatgtac agtatttatt
     2881 tttaacttat tgttttaaaa gtcaaagagg attttataat ggaaaccgcg gtagcgtact
     2941 ataaagatgg tgttccttat gatgataagg gacaggtaat tattactctt ttgaatggta
     3001 ctcctgacgg gagtggctct ggcggcggag gtggaaaagg aggcagtaaa agtgaaagtt
     3061 ctgcagctat tcatgcaact gctaaatggt ctactgctca attaaagaaa acacaggcag
     3121 agcaggctgc ccgggcaaaa gctgcagcgg aagcacaggc gaaagcaaag gcaaacaggg
     3181 atgcgctgac tcagcgcctg aaggatatcg tgaatgaggc tcttcgtcac aatgcctcac
     3241 gtacgccttc agcaacagag cttgctcatg ctaataatgc agctatgcag gcggaagcag
     3301 agcgtttgcg ccttgcgaaa gcagaagaaa aagcccgtaa agaagcggaa gcagcagaaa
     3361 aggcttttca ggaagcagaa caacgacgta aagagattga acgggagaag gctgaaacag
     3421 aacgccagtt gaaactggct gaagctgaag agaaacgact ggctgcattg agtgaagaag
     3481 ctaaagctgt tgagatcgcc caaaaaaaac tttctgctgc acaatctgaa gtggtgaaaa
     3541 tggatggaga gattaagact ctcaattctc gtttaagctc cagtatccat gcccgtgatg
     3601 cagaaatgaa aacgctcgct ggaaaacgaa atgaactggc tcaggcatcc gctaaatata
     3661 aagaactgga tgagctggtc aaaaaactat caccaagagc caatgatccg cttcagaacc
     3721 gtcctttttt tgaagcaacc agacgacggg ttggggccgg taagattaga gaagaaaaac
     3781 aaaaacaggt aacagcatca gaaacacgta ttaaccggat aaatgctgat ataactcaga
     3841 tccagaaggc tatttctcag gtcagtaata atcgtaatgc cggtatcgct cgtgttcatg
     3901 aagctgaaga aaatttgaaa aaagcacaga ataatctcct taattcacag attaaggatg
     3961 ctgttgatgc aacagttagc ttttatcaaa cgctgactga aaaatatggt gaaaaatatt
     4021 cgaaaatggc acaggaactt gctgataagt ctaaaggtaa gaaaatcggc aatgtgaatg
     4081 aagctctcgc tgcttttgaa aaatacaagg atgttttaaa taagaaattc agcaaagccg
     4141 atcgtgatgc tatttttaat gcgttggcat cggtgaagta tgatgactgg gctaaacatt
     4201 tagatcagtt tgccaagtac ttgaagatta cggggcatgt ttcttttgga tatgatgtgg
     4261 tatctgatat cctaaaaatt aaggatacag gtgactggaa gccactattt cttacattag
     4321 agaagaaagc tgcagatgca ggggtgagtt atgttgttgc tttacttttt agcttgcttg
     4381 ctggaactac attaggtatt tggggtattg ctattgttac aggaattcta tgctcctata
     4441 ttgataagaa taaacttaat actataaatg aggtgttagg gatttaatta ttctttattg
     4501 atataaaaag tcctagcaat ccaaatggga ttgctaggac caaacaaagt agattatata
     4561 gcataaatag gtttaatttt gctacggggg cgttatttag gttttttctt ctttcgaaaa
     4621 aatctttctt tatgaagtta aaagctatgt attcaatagc atattttgaa tatggacata
     4681 gaatagtgct tatcactatt gcatatagca tcttatctga cacaaggaaa taataccctt
     4741 cgctgttttt tgttataagg tatatatata taagtgtgca gtacaggcca aataaaatat
     4801 tttttatgta gtatcttaag ctcataaatt aaacctcgcc atatattctt ttcattttat
     4861 aaggatcgag ttatgaggaa aagatttttt gtgggaatat tcgcgataaa cctccttgtt
     4921 ggatgtcagg ctaactatat acgtgatgtt cagggaggga ccatcgcacc atcctcctct
     4981 tctaaactga cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt
     5041 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca acggtggttt
     5101 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5161 agataccaaa tactgtcctt ctagtgtagc cgtagtcggg ccactacttc aagaactctg
     5221 tagcaccgtt tgtgccatca tcgctctgct aatccggtta ccagtggctg ctgccagtgg
     5281 cgttaaggcg tgccttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5341 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5401 actgagatac caacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5461 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5521 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtct
     5581 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgcctgct acgtggcctt
     5641 cttcctgttc ctggtctttt gctcacatgt tctttccggc cttatcccct gattctgtgg
     5701 ataactgtgt taccgttttt gtgtgagtca gtaccgctcg ccgcagtcga acgaccgagc
     5761 gtagcgagtc agtgagcgag gaagcggaaa agcgcctgga cgtgcatttt ctccttacgc
     5821 atctgtgcgg catttcacac ccggcatggc gtacttttca tacaatccgc actgatgccg
     5881 catggttaag ccagtataca ctccgctatc gctacgtgac tgggtcaggg ctgcgccccg
     5941 acacccgcta aaacctgctg acgcgccctg acgggcttgt cagctcccgg catccgctca
     6001 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcccc
     6061 gaaacgtgcg aggcagctgc ggtaaagctc atcggcgtgg tcgtgaagcg attcacaaat
     6121 atcggcctgt tcatctgcgt ccagttcgtt gagcttctcc agcagcgtta atgtctggct
     6181 tctgataaag cgggccatgt taagggcggt tttttcctgt ttagtcactg atgcctccgt
     6241 gtaaggggga tttctgttca tggggtaatg ataccgatga aacgcgagag gatgctcaca
     6301 atacgggtta ctgatgatga acatgcccgg ttacttgaac gctgtgaggg taaacaactg
     6361 gcggtatgga tgcggcgggt ctgcctgggg gagccggttg cccgttccgg aaaactgccg
     6421 acactggcac cgccgttact gcgtcagctg gccgccatcg gaaataacct gaatcagaca
     6481 gcccgtaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt ggtgacgcac
     6541 tgataccatc ggggatgagc tgcgccggct gcgtctggct gtcagggaac agggggcgcg
     6601 ggatgatagt taaatttcat gccaggggaa aaggtggtgg cagtg
//
