LOCUS       OY856298                6646 bp    DNA     circular BCT 04-DEC-2023
DEFINITION  Escherichia coli strain EC17522023BGR genome assembly, plasmid:
            P1752_2.
ACCESSION   OY856298
VERSION     OY856298.1
DBLINK      BioProject: PRJEB70793
            BioSample: SAMEA114783440
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ivanov,I., Teneva,D. and Stoikov,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2023) NCIPD, CMAR, Yanko Sakazov 26 Sofia,
            Bulgaria
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC17522023BGR"
                     /isolate="EC17522023"
                     /isolation_source="human urine"
                     /db_xref="taxon:562"
                     /plasmid="P1752_2"
     gene            468..986
                     /gene="mbeB"
                     /locus_tag="ECO1752_28640"
     CDS             468..986
                     /gene="mbeB"
                     /locus_tag="ECO1752_28640"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_000067500.1"
                     /note="RefSeq:WP_000067500.1; SO:0001217;
                     UniParc:UPI0000001574; UniRef:UniRef100_P13659;
                     UniRef:UniRef50_P13659; UniRef:UniRef90_P13659"
                     /codon_start=1
                     /transl_table=11
                     /product="Mobilization protein MbeB"
                     /protein_id="CAK5471222.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mbeB"
     gene            993..1226
                     /gene="mbeD"
                     /locus_tag="ECO1752_28645"
     CDS             993..1226
                     /gene="mbeD"
                     /locus_tag="ECO1752_28645"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_000137445.1"
                     /note="RefSeq:WP_000137445.1; SO:0001217;
                     UniParc:UPI0000000949; UniRef:UniRef100_P13660;
                     UniRef:UniRef50_P13660; UniRef:UniRef90_P13660"
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="CAK5471223.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            1257..1418
                     /gene="mbeE"
                     /locus_tag="ECO1752_28650"
     CDS             1257..1418
                     /gene="mbeE"
                     /locus_tag="ECO1752_28650"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_001405928.1"
                     /note="RefSeq:WP_001405928.1; SO:0001217;
                     UniParc:UPI00005FF682; UniRef:UniRef100_G9BDI8;
                     UniRef:UniRef50_D8A0P8; UniRef:UniRef90_G9BDI8"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase MbeE"
                     /protein_id="CAK5471224.1"
                     /translation="MCYPWNGSVSRKYRNARWMALRWDGETMKNGSFSGLSGGNVACTL
                     WLHRYSYG"
                     /name="mbeE"
     gene            complement(1544..1963)
                     /locus_tag="ECO1752_28655"
     CDS             complement(1544..1963)
                     /locus_tag="ECO1752_28655"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_001254967.1"
                     /note="RefSeq:WP_001254967.1; SO:0001217;
                     UniParc:UPI00000B019C; UniRef:UniRef100_Q51623;
                     UniRef:UniRef50_F4T8P0; UniRef:UniRef90_Q51623"
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="CAK5471225.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2044..2481)
                     /locus_tag="ECO1752_28660"
     CDS             complement(2044..2481)
                     /locus_tag="ECO1752_28660"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_001405929.1"
                     /note="RefSeq:WP_001405929.1; SO:0001217;
                     UniParc:UPI00025CB33B; UniRef:UniRef100_A0A2P9E2X6;
                     UniRef:UniRef50_Q51610; UniRef:UniRef90_A0A5B9SUN1"
                     /codon_start=1
                     /transl_table=11
                     /product="Entry exclusion protein 1"
                     /protein_id="CAK5471226.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="Entry exclusion protein 1"
     gene            2741..4309
                     /gene="cea"
                     /locus_tag="ECO1752_28665"
     CDS             2741..4309
                     /gene="cea"
                     /locus_tag="ECO1752_28665"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_000447006.1"
                     /note="BlastRules:WP_000447006; RefSeq:WP_000447006.1;
                     SO:0001217; UniParc:UPI00000BB5A2; UniRef:UniRef100_Q51626;
                     UniRef:UniRef50_P02978; UniRef:UniRef90_P02978"
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="CAK5471227.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(4306..4647)
                     /gene="imm"
                     /locus_tag="ECO1752_28670"
     CDS             complement(4306..4647)
                     /gene="imm"
                     /locus_tag="ECO1752_28670"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_000058760.1"
                     /note="RefSeq:WP_000058760.1; SO:0001217;
                     UniParc:UPI00000032F4; UniRef:UniRef100_P02985;
                     UniRef:UniRef50_P02985; UniRef:UniRef90_P02985"
                     /codon_start=1
                     /transl_table=11
                     /product="Colicin-E1 immunity protein"
                     /protein_id="CAK5471228.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="imm"
     gene            4695..4832
                     /locus_tag="ECO1752_28675"
     CDS             4695..4832
                     /locus_tag="ECO1752_28675"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_001405930.1"
                     /note="RefSeq:WP_001405930.1; SO:0001217;
                     UniParc:UPI00000032F5; UniRef:UniRef100_P05821;
                     UniRef:UniRef90_P05821"
                     /codon_start=1
                     /transl_table=11
                     /product="Lysis protein for colicin E1"
                     /protein_id="CAK5471230.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="Lysis protein for colicin E1"
     rep_origin      4828..5858
                     /inference="similar to DNA sequence"
                     /note="origin of replication"
     gene            complement(4892..4996)
                     /gene="RNAI"
                     /locus_tag="ECO1752_28680"
     ncRNA           complement(4892..4996)
                     /ncRNA_class="other"
                     /gene="RNAI"
                     /locus_tag="ECO1752_28680"
                     /product="RNAI"
                     /inference="profile:Rfam:RF00106"
                     /note="SO:0000655"
     oriT            5686..5774
                     /inference="similar to DNA sequence"
                     /note="origin of transfer"
     gene            complement(5859..6050)
                     /gene="rop"
                     /locus_tag="ECO1752_28685"
     CDS             complement(5859..6050)
                     /gene="rop"
                     /locus_tag="ECO1752_28685"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_000165985.1"
                     /note="RefSeq:WP_000165985.1; SO:0001217;
                     UniParc:UPI00000000E5; UniRef:UniRef100_P03051;
                     UniRef:UniRef50_P03051; UniRef:UniRef90_P03051"
                     /codon_start=1
                     /transl_table=11
                     /product="Regulatory protein rop"
                     /protein_id="CAK5471232.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="rop"
     gene            6099..6365
                     /gene="mobC"
                     /locus_tag="ECO1752_28690"
     CDS             6099..6365
                     /gene="mobC"
                     /locus_tag="ECO1752_28690"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA
                     sequence:UniRef:UniRef90_UPI001F359810"
                     /note="SO:0001217; UniRef:UniRef50_A0A1X0Y5G7;
                     UniRef:UniRef90_UPI001F359810"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="CAK5471234.1"
                     /translation="MKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSG
                     KLPTLAPPLLRQLTAIGNNLNQTARKVNSGQWSSGDRVQVVAH"
                     /name="mobC"
     gene            6426..>6644
                     /locus_tag="ECO1752_28695"
     CDS             6426..>6644
                     /locus_tag="ECO1752_28695"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA
                     sequence:UniRef:UniRef90_UPI0012FF5AED"
                     /note="SO:0001217; UniRef:UniRef50_A0A2S8DAJ8;
                     UniRef:UniRef90_UPI0012FF5AED"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAK5471236.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKV"
                     /name="hypothetical protein"
     Typing          5687..5774
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(5118..5673)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.699"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3..1330
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.222"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          6085..6359
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.739"
                     /coverage="99.0"
                     /evalue="3.44e-53"
     Typing          6427..6644
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.72e-39"
ORIGIN
        1 ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg
       61 ggtggagcac caggacaagg gacggcttga gctgaatttt gtcattccga acatggagct
      121 acagaccgga aaacgcctcc agccgtacta cgaccgcgca gacaggccta gaattgatgc
      181 ctggcagacg ctggtgaatc accattacgg gctgcatgac ccgaacgccc cggagaaccg
      241 caggacgctg acactccctg ataacctgcc tgaaacgaaa caggcgcttg ctgagggcgt
      301 cacgcgaggt atagatgcac tttaccatgc cggagagata aaaggccgtc aggatgtgat
      361 tcaggcgctc actgaggcgg ggctggaagt ggtcagggtg acgcgaagca gtatcagcat
      421 tgcagatccg aacggcggga agaatatcag gctgaaagga gcattttatg agcaatcttt
      481 tgcagacggg cgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa
      541 tgctgaacaa cgagttcagg aggctcggcg aatctgtaag cgaggctgtg acatcaaacg
      601 agacgaaaat cagagacgct atagccctgt tcacagcctc gacagaggaa tcgctggaaa
      661 aacaccggga aggggtgaaa gaggcgatga tgcagcacag gagggacgtg ttaaagctgg
      721 cagggaatac gggcatgatg ttactgggga tagtctttct cctgtttacc gcgagtggcg
      781 ggacgctctg gtatcttgga gggaggatac aggcgaacct ggaagaaatc aggaagcagg
      841 aagagacatt gcagaaactg aacgcgaaga catggggcgt ggagtttgtg caggacggga
      901 acaggaaatt ccttgtcctt ccgtacggga aatcagcgga ggtgattccc tttcagggga
      961 aagagtgggt acatctgaag gagtgacaca gagtgacaga gctggaaaca catttgctga
     1021 acgccttaga gcagctgcaa caggactata tgcagcggct gagcgaatgg gagagcgcct
     1081 tcgtggaatt gcagaagatg ttttcgctta cgcaacggga caacgcgatg ctgaacgagc
     1141 gggtcatgca gttgagtcag caggtgcagc acttgagcga gcagacagaa cgcttgagcc
     1201 agttatacag cgagaactgg agataagaga ggaacggctg atacaggagc gcgaacatgt
     1261 gttatccctg gaacgggagc gtcagccgga aatacaggaa cgcacgctgg atggcccttc
     1321 gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg ggtaatgtgg
     1381 cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc gcgatcgcgg
     1441 cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc gcgctgaacg
     1501 cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt ctgccctcgt
     1561 agccatcgag ataaaccgca gaaatcgtgt cagccagcag cctggatttt ttcggggtaa
     1621 ggtctttgct gcggtaatca gtacccgcaa attgagttgt gccccggagt gagtttaaaa
     1681 attcctggct gtgaaactgc tgaatgcgtt gttgcacatc actccgtgat tttcctgtcg
     1741 ctttatcttt agctcccata tcccagaact gccgaaaaaa aggcaccatc attcgtgttg
     1801 tgtcggcttt ttgggtggaa aagttgggat caaaaccgtc atcagaagca tatacgtaat
     1861 gccgggtatg ttgttccacg ctgccctttg gggtacaggc tgtgagagct actgttgctg
     1921 ttatcatggc gataagacaa agtttgtttc cggtacgtct catgagtgtc tccctacctt
     1981 aaagtattta ttctcggtga gttattatcc gaagactatt ttatcatcag tctctgacag
     2041 cgttcagcga ccgaacaacc tggaccagaa tccccgtttc ttttccagtt ccagtgcctg
     2101 cctgagctgg gctatctcat tttgtagctg ttcccgttct gcttcctggc gtctgcgatc
     2161 catatcctgt gcctgtttat cctcaagcat cagcgtcagg cattgtttca gctcattcag
     2221 ctcccggaga atgcgttctg tctgctgatc atgtggattt tctgcatgtc cctcactgtg
     2281 cctttctggt gtctcattct gctttaattc gccgtatgcc cggatcagtt cactggtttc
     2341 aaactcccgt cgtccgtccc tgccggtacg gtaactgaca aggcctgcgc gcatatcacg
     2401 gtaaaactgt gaacgcgatc tgcctgtcat ttttagtgcg tcccggagtg tgtgccatgc
     2461 cataaagtga cagtgtccca tagatgtctc atctcatagt ttcagtaaaa cataatgagg
     2521 tctgagaacg gtaatgtttg tgctggtttt tgtggcatcg ggcgagaata gcgcgtggtg
     2581 tgaaagactg tttttttgat cgttttcaca aaaatggaag tccacagtct tgacagggaa
     2641 aatgcagcgg cgtagctttt atgctgtata taaaaccagt ggttatatgt acagtattta
     2701 tttttaactt attgttttaa aagtcaaaga ggattttata atggaaaccg cggtagcgta
     2761 ctataaagat ggtgttcctt atgatgataa gggacaggta attattactc ttttgaatgg
     2821 tactcctgac gggagtggct ctggcggcgg aggtggaaaa ggaggcagta aaagtgaaag
     2881 ttctgcagct attcatgcaa ctgctaaatg gtctactgct caattaaaga aaacacaggc
     2941 agagcaggct gcccgggcaa aagctgcagc ggaagcacag gcgaaagcaa aggcaaacag
     3001 ggatgcgctg actcagcgcc tgaaggatat cgtgaatgag gctcttcgtc acaatgcctc
     3061 acgtacgcct tcagcaacag agcttgctca tgctaataat gcagctatgc aggcggaagc
     3121 agagcgtttg cgccttgcga aagcagaaga aaaagcccgt aaagaagcgg aagcagcaga
     3181 aaaggctttt caggaagcag aacaacgacg taaagagatt gaacgggaga aggctgaaac
     3241 agaacgccag ttgaaactgg ctgaagctga agagaaacga ctggctgcat tgagtgaaga
     3301 agctaaagct gttgagatcg cccaaaaaaa actttctgct gcacaatctg aagtggtgaa
     3361 aatggatgga gagattaaga ctctcaattc tcgtttaagc tccagtatcc atgcccgtga
     3421 tgcagaaatg aaaacgctcg ctggaaaacg aaatgaactg gctcaggcat ccgctaaata
     3481 taaagaactg gatgagctgg tcaaaaaact atcaccaaga gccaatgatc cgcttcagaa
     3541 ccgtcctttt tttgaagcaa ccagacgacg ggttggggcc ggtaagatta gagaagaaaa
     3601 acaaaaacag gtaacagcat cagaaacacg tattaaccgg ataaatgctg atataactca
     3661 gatccagaag gctatttctc aggtcagtaa taatcgtaat gccggtatcg ctcgtgttca
     3721 tgaagctgaa gaaaatttga aaaaagcaca gaataatctc cttaattcac agattaagga
     3781 tgctgttgat gcaacagtta gcttttatca aacgctgact gaaaaatatg gtgaaaaata
     3841 ttcgaaaatg gcacaggaac ttgctgataa gtctaaaggt aagaaaatcg gcaatgtgaa
     3901 tgaagctctc gctgcttttg aaaaatacaa ggatgtttta aataagaaat tcagcaaagc
     3961 cgatcgtgat gctattttta atgcgttggc atcggtgaag tatgatgact gggctaaaca
     4021 tttagatcag tttgccaagt acttgaagat tacggggcat gtttcttttg gatatgatgt
     4081 ggtatctgat atcctaaaaa ttaaggatac aggtgactgg aagccactat ttcttacatt
     4141 agagaagaaa gctgcagatg caggggtgag ttatgttgtt gctttacttt ttagcttgct
     4201 tgctggaact acattaggta tttggggtat tgctattgtt acaggaattc tatgctccta
     4261 tattgataag aataaactta atactataaa tgaggtgtta gggatttaat tattctttat
     4321 tgatataaaa agtcctagca atccaaatgg gattgctagg accaaacaaa gtagattata
     4381 tagcataaat aggtttaatt ttgctacggg ggcgttattt aggttttttc ttctttcgaa
     4441 aaaatctttc tttatgaagt taaaagctat gtattcaata gcatattttg aatatggaca
     4501 tagaatagtg cttatcacta ttgcatatag catcttatct gacacaagga aataataccc
     4561 ttcgctgttt tttgttataa ggtatatata tataagtgtg cagtacaggc caaataaaat
     4621 attttttatg tagtatctta agctcataaa ttaaacctcg ccatatattc ttttcatttt
     4681 ataaggatcg agttatgagg aaaagatttt ttgtgggaat attcgcgata aacctccttg
     4741 ttggatgtca ggctaactat atacgtgatg ttcagggagg gaccatcgca ccatcctcct
     4801 cttctaaact gacggggatc gcggttcagt agaaaagatc aaaggatctt cttgagatcc
     4861 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac caacggtggt
     4921 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4981 gcagatacca aatactgtcc ttctagtgta gccgtagtcg ggccactact tcaagaactc
     5041 tgtagcaccg tttgtgccat catcgctctg ctaatccggt taccagtggc tgctgccagt
     5101 ggcgttaagg cgtgccttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5161 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5221 gaactgagat accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5281 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5341 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5401 ctatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgcctg ctacgtggcc
     5461 ttcttcctgt tcctggtctt ttgctcacat gttctttccg gccttatccc ctgattctgt
     5521 ggataactgt gttaccgttt ttgtgtgagt cagtaccgct cgccgcagtc gaacgaccga
     5581 gcgtagcgag tcagtgagcg aggaagcgga aaagcgcctg gacgtgcatt ttctccttac
     5641 gcatctgtgc ggcatttcac acccggcatg gcgtactttt catacaatcc gcactgatgc
     5701 cgcatggtta agccagtata cactccgcta tcgctacgtg actgggtcag ggctgcgccc
     5761 cgacacccgc taaaacctgc tgacgcgccc tgacgggctt gtcagctccc ggcatccgct
     5821 cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatcc
     5881 ccgaaacgtg cgaggcagct gcggtaaagc tcatcggcgt ggtcgtgaag cgattcacaa
     5941 atatcggcct gttcatctgc gtccagttcg ttgagcttct ccagcagcgt taatgtctgg
     6001 cttctgataa agcgggccat gttaagggcg gttttttcct gtttagtcac tgatgcctcc
     6061 gtgtaagggg gatttctgtt catggggtaa tgataccgat gaaacgcgag aggatgctca
     6121 caatacgggt tactgatgat gaacatgccc ggttacttga acgctgtgag ggtaaacaac
     6181 tggcggtatg gatgcggcgg gtctgcctgg gggagccggt tgcccgttcc ggaaaactgc
     6241 cgacactggc accgccgtta ctgcgtcagc tgaccgccat cggaaataac ctgaatcaga
     6301 cagcccgtaa ggtgaacagc gggcagtggt cttccggtga ccgggttcag gtggtggcgc
     6361 actgatggcc atcggggatg agctgcgccg gctgcgtctg gctgtcaggg aacagggggc
     6421 gcgggatgat agttaaattt catgccaggg gaaaaggtgg tggcagtggt ccggttgatt
     6481 acctgctggg gagggagcgt aaccgcgaag gcgcaacggt gcttcagggt aatccggaag
     6541 aagtccggga actcatcgat gccacgccat ttgcgaagaa atacacgtcc ggtgttctgt
     6601 cgttcgcgga gaaggagctg ccgccgggag gacgtgaaaa agtgat
//
