LOCUS       OW969869               13636 bp    DNA     circular BCT 22-MAY-2022
DEFINITION  Klebsiella pneumoniae isolate 512 genome assembly, plasmid: P4.
ACCESSION   OW969869
VERSION     OW969869.1
DBLINK      BioProject: PRJEB42440
            BioSample: SAMEA8158478
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="512"
                     /isolation_source="Lab. Dr. Echevarne (Pontevedra)"
                     /db_xref="taxon:573"
                     /plasmid="P4"
     gene            complement(929..1057)
                     /locus_tag="AI3046V2_5425"
     mRNA            complement(929..1057)
                     /locus_tag="AI3046V2_5425"
                     /note="AI3046v2_5425.mrna"
     CDS             complement(929..1057)
                     /locus_tag="AI3046V2_5425"
                     /note="long variant; AI3046v2_5425.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH6290201.1"
                     /translation="MEKRHLRGLAVLPHMLVPCLFTESVGNFACVRSARRSLTAGA"
                     /name="hypothetical protein"
     exon            complement(929..1057)
                     /locus_tag="AI3046V2_5425"
                     /note="AI3046v2_5425.mrna"
                     /number=1
     gene            complement(1932..2189)
                     /locus_tag="AI3046V2_5426"
     mRNA            complement(1932..2189)
                     /locus_tag="AI3046V2_5426"
                     /note="AI3046v2_5426.mrna"
     CDS             complement(1932..2189)
                     /locus_tag="AI3046V2_5426"
                     /note="long variant; AI3046v2_5426.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Colicin-E3 immunity protein"
                     /protein_id="CAH6290242.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="Colicin-E3 immunity protein"
     exon            complement(1932..2189)
                     /locus_tag="AI3046V2_5426"
                     /note="AI3046v2_5426.mrna"
                     /number=1
     gene            complement(2199..>2735)
                     /locus_tag="AI3046V2_5427"
     mRNA            complement(2199..2735)
                     /locus_tag="AI3046V2_5427"
                     /note="AI3046v2_5427.mrna"
     CDS             complement(2199..>2735)
                     /locus_tag="AI3046V2_5427"
                     /note="short variant; AI3046v2_5427.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Colicin-E3"
                     /protein_id="CAH6290284.1"
                     /translation="VTYNPPHEYGSGWQDQVRYLDKDIQNQNEKLKAAQTSLNEMNESL
                     SRDKAALSGAMESRKQKEKKAKDAENKLNEEKKKPRKGTKDYGHDYFPDPKTEDIKGLG
                     ELKEGKPKTPKQGGGGKRARWYGDKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGK
                     QVKGPDPKRNIKKYL"
                     /name="Colicin-E3"
     exon            complement(2199..2735)
                     /locus_tag="AI3046V2_5427"
                     /note="AI3046v2_5427.mrna"
                     /number=1
     gene            complement(3156..3860)
                     /locus_tag="AI3046V2_5428"
     mRNA            complement(3156..3860)
                     /locus_tag="AI3046V2_5428"
                     /note="AI3046v2_5428.mrna"
     CDS             complement(3156..3860)
                     /locus_tag="AI3046V2_5428"
                     /note="long variant; AI3046v2_5428.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH6290288.1"
                     /translation="MNPFKGRHFQRDIIRWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="hypothetical protein"
     exon            complement(3156..3860)
                     /locus_tag="AI3046V2_5428"
                     /note="AI3046v2_5428.mrna"
                     /number=1
     gene            complement(3872..>4129)
                     /locus_tag="AI3046V2_5429"
     mRNA            complement(3872..4129)
                     /locus_tag="AI3046V2_5429"
                     /note="AI3046v2_5429.mrna"
     CDS             complement(3872..>4129)
                     /locus_tag="AI3046V2_5429"
                     /note="short variant; AI3046v2_5429.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptomycin 3''-adenylyltransferase"
                     /protein_id="CAH6290328.1"
                     /translation="VIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDID
                     LLVTVTVRLDETTRRALINDLLETALLQIVGGDKLIIPFC"
                     /name="Streptomycin 3''-adenylyltransferase"
     exon            complement(3872..4129)
                     /locus_tag="AI3046V2_5429"
                     /note="AI3046v2_5429.mrna"
                     /number=1
     gene            complement(4211..4816)
                     /locus_tag="AI3046V2_5430"
     mRNA            complement(4211..4816)
                     /locus_tag="AI3046V2_5430"
                     /note="AI3046v2_5430.mrna"
     CDS             complement(4211..4816)
                     /locus_tag="AI3046V2_5430"
                     /note="long variant; AI3046v2_5430.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside N(6')-acetyltransferase type 1"
                     /protein_id="CAH6290367.1"
                     /translation="MSIQHFQTKLGITKYSIVTNSNDSVTLRLMTEHDLAMLYEWLNRS
                     HIVEWWGGEEARPTLADVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGW
                     WEEETDPGVRGIDQLLANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAI
                     RCYEKAGFERQGTVTTPDGPAVYMVQTRQAFERTRSVA"
                     /name="Aminoglycoside N(6')-acetyltransferase type 1"
     exon            complement(4211..4816)
                     /locus_tag="AI3046V2_5430"
                     /note="AI3046v2_5430.mrna"
                     /number=1
     gene            complement(4999..5556)
                     /locus_tag="AI3046V2_5431"
     mRNA            complement(4999..5556)
                     /locus_tag="AI3046V2_5431"
                     /note="AI3046v2_5431.mrna"
     CDS             complement(4999..5556)
                     /locus_tag="AI3046V2_5431"
                     /note="long variant; AI3046v2_5431.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Transposon Tn3 resolvase"
                     /protein_id="CAH6290373.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="Transposon Tn3 resolvase"
     exon            complement(4999..5556)
                     /locus_tag="AI3046V2_5431"
                     /note="AI3046v2_5431.mrna"
                     /number=1
     gene            5718..8723
                     /locus_tag="AI3046V2_5432"
     mRNA            5718..8723
                     /locus_tag="AI3046V2_5432"
                     /note="AI3046v2_5432.mrna"
     CDS             5718..8723
                     /locus_tag="AI3046V2_5432"
                     /note="long variant; AI3046v2_5432.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH6290410.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="hypothetical protein"
     exon            5718..8723
                     /locus_tag="AI3046V2_5432"
                     /note="AI3046v2_5432.mrna"
                     /number=1
     gene            <8716..9048
                     /locus_tag="AI3046V2_5433"
     mRNA            8716..9048
                     /locus_tag="AI3046V2_5433"
                     /note="AI3046v2_5433.mrna"
     CDS             <8716..9048
                     /locus_tag="AI3046V2_5433"
                     /note="short variant; AI3046v2_5433.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH6290452.1"
                     /translation="LLNVSFRSTERQTPFDPNFSTQKADTTRMMVPFFRQFWDMGAKDK
                     ATGKSRSDVQQRIQQFHSQEFLNSLQGTTQFAGTDYRSKDLTPKKSRLLADTISAVYLD
                     GYEGRQ"
                     /name="hypothetical protein"
     exon            8716..9048
                     /locus_tag="AI3046V2_5433"
                     /note="AI3046v2_5433.mrna"
                     /number=1
     gene            9354..9617
                     /locus_tag="AI3046V2_5434"
     mRNA            9354..9617
                     /locus_tag="AI3046V2_5434"
                     /note="AI3046v2_5434.mrna"
     CDS             9354..9617
                     /locus_tag="AI3046V2_5434"
                     /note="long variant; AI3046v2_5434.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH6290489.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     exon            9354..9617
                     /locus_tag="AI3046V2_5434"
                     /note="AI3046v2_5434.mrna"
                     /number=1
     gene            9607..9906
                     /locus_tag="AI3046V2_5435"
     mRNA            9607..9906
                     /locus_tag="AI3046V2_5435"
                     /note="AI3046v2_5435.mrna"
     CDS             9607..9906
                     /locus_tag="AI3046V2_5435"
                     /note="long variant; AI3046v2_5435.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH6290526.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="hypothetical protein"
     exon            9607..9906
                     /locus_tag="AI3046V2_5435"
                     /note="AI3046v2_5435.mrna"
                     /number=1
     gene            complement(9968..10345)
                     /locus_tag="AI3046V2_5436"
     mRNA            complement(9968..10345)
                     /locus_tag="AI3046V2_5436"
                     /note="AI3046v2_5436.mrna"
     CDS             complement(9968..10345)
                     /locus_tag="AI3046V2_5436"
                     /note="long variant; AI3046v2_5436.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH6290563.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     exon            complement(9968..10345)
                     /locus_tag="AI3046V2_5436"
                     /note="AI3046v2_5436.mrna"
                     /number=1
     gene            10414..10593
                     /locus_tag="AI3046V2_5437"
     mRNA            10414..10593
                     /locus_tag="AI3046V2_5437"
                     /note="AI3046v2_5437.mrna"
     CDS             10414..10593
                     /locus_tag="AI3046V2_5437"
                     /note="long variant; AI3046v2_5437.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Regulatory protein rop"
                     /protein_id="CAH6290599.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Regulatory protein rop"
     exon            10414..10593
                     /locus_tag="AI3046V2_5437"
                     /note="AI3046v2_5437.mrna"
                     /number=1
     gene            complement(10621..11151)
                     /locus_tag="AI3046V2_5438"
     mRNA            complement(10621..11151)
                     /locus_tag="AI3046V2_5438"
                     /note="AI3046v2_5438.mrna"
     CDS             complement(10621..11151)
                     /locus_tag="AI3046V2_5438"
                     /note="long variant; AI3046v2_5438.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH6290637.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     exon            complement(10621..11151)
                     /locus_tag="AI3046V2_5438"
                     /note="AI3046v2_5438.mrna"
                     /number=1
     gene            complement(11158..11889)
                     /locus_tag="AI3046V2_5439"
     mRNA            complement(11158..11889)
                     /locus_tag="AI3046V2_5439"
                     /note="AI3046v2_5439.mrna"
     CDS             complement(11158..11889)
                     /locus_tag="AI3046V2_5439"
                     /note="long variant; AI3046v2_5439.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH6290675.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="hypothetical protein"
     exon            complement(11158..11889)
                     /locus_tag="AI3046V2_5439"
                     /note="AI3046v2_5439.mrna"
                     /number=1
     gene            complement(11889..13574)
                     /locus_tag="AI3046V2_5440"
     mRNA            complement(11889..13574)
                     /locus_tag="AI3046V2_5440"
                     /note="AI3046v2_5440.mrna"
     CDS             complement(11889..13574)
                     /locus_tag="AI3046V2_5440"
                     /note="long variant; AI3046v2_5440.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH6290713.1"
                     /translation="MVRARRHVWNYAYWLTGAAGLALLGGLVVAVFIESWGETVLRPDL
                     YANADYYLLQPANLFGIAWCAGGLAVGIVLARLLAFLLLDGHFAAADEAVNAKLNQESR
                     RSSQRTGEMTDVRHLHFGEPVPVNALADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWR
                     KTNIQILGLPGSGKSVMATNALIRCVRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLD
                     LRPGKPAQLNLFRDLDQYALKNLLIAGLNLGETGDVADHYRISEQKAAKLIAEQFPQGA
                     NIQQVLAAAYALPEALKKDVKGLITRLENVADLSVLQTDSGIDVAGIINGGGCLYVIGS
                     MDDEAVIRVQKMLFARCAQIIIARDEFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRD
                     CNLLLAHQSLGDFGQCGQDLPAEFVKTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRV
                     DVERRRASREAGNVEHISGDSFIQKEARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSP
                     LRVERREIPLQRFPVLAKTDPLAEFRPPADGGGPKADDDFAGIY"
                     /name="hypothetical protein"
     exon            complement(11889..13574)
                     /locus_tag="AI3046V2_5440"
                     /note="AI3046v2_5440.mrna"
                     /number=1
     ARG             complement(4215..4765)
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3873..4129)
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             complement(4212..4816)
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     Typing          1017..1595
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11162..11889)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 tatagcgatc ggcatactcc cgtcgctcct tatgactcag ccccacaaaa cgcatccgtc
       61 cggcccaaag ttcagccaga ccgaaaaaca ggccgataac taggaaagcc ccggccagac
      121 cgtaatactc ctggtacagc cagctgaccc acggataaat ctgccgggcc agctccggaa
      181 tgtgccactt ccaggcaagc gaatccgtat taaacatgtt ctctctcctt cagacgatgg
      241 tttgccggcg tgatgtgcag cccccgctgc acaagccgcg gacatgcccc aaggcatggc
      301 ggcggcggtc acgtccgtgg tgattgcgtt cagctttcac cacggaggcc agcctctttc
      361 cgccccatgc ggaaaaggcg cagccccgga ggaacaacga gcacgcccca gcgtgtgaga
      421 ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc ggcccccgtc agagttcgcc
      481 atagcgagcg gctgaccacc cgcaaaatca ggagcagctt tgctgcttcg ccaggtgcgt
      541 ccagcaccgt ggatttttgt gggtggtcag gcgtgagcct gacgggggcc gggtgaattt
      601 ctggcgtggc cggcgtccag ccggacggag ccagaaagag ggggagagac cgtgcgtcgg
      661 tggcgtccag ccagcgtaag caggggatat ccccctgagg tcagccacgc cctcgcgggg
      721 atgacctgta tttctccgta aggagaaaaa agcaacagag ataggttaac gtaaaatgtt
      781 cagcgaaaaa ccgatatgat tcacctatcc tggtaattta ttatcaggga atataaagac
      841 ttaatcaggg gacaatatgc cacatcagcg cgtccggtgt ccgtcttgta ggtgccgggg
      901 aaattgcgcc tcctcgctca ctcggtcgct acgctccggc cgtgagactg cggcgggcgc
      961 tgcggacaca tgcaaagtta cccacagatt ccgtgaataa gcaggggact aacatgtgag
     1021 gcaaaacggc aaggccacgc aggtggcgtt tttccatagg ctccgccctc ctgactgagt
     1081 tcacataaac agacgctttt tcggtgcatc tgtgggagcc ggtggctcaa ccatgaatca
     1141 gtcaggagtg gcgaaacccg acaggactta aagatcatca ccgtttccga tgaatcgctc
     1201 cctcttgcgc tctcctgttc caaccctgcc gtttcccgga tacctgttcc gcctttctcc
     1261 cttgcgggaa gtgtggcgct ttctcatagc tcacactctg gtatctcggc tcggtgtagg
     1321 tcgttcgctc caagctgggc tgtaagcaag aaccccccgt tcagcccgac tgctgcgcct
     1381 tatccggtaa ctgttcactt gagtccaacc cggaaaagca cgcaaaacgc cactggcagc
     1441 agccattggt aactgggagt tcgcagagga tttgtttagc taaacacgcg gttgctcttg
     1501 aagtgtgcgc caaagtccgg ctacactgga aggacagatt tggttgctgt gctctgcgaa
     1561 agccagttac cacggttaag cagttcccca actgacttaa ccttcgatca aaccacctcc
     1621 ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga
     1681 agatcctttg atcttttcta ctgaaccgcg atccccgtca gttcagagga ggacgatggt
     1741 gccaccgttc caccctgaac atcccggata tagtttgcct gacatgcgac cagaagaaag
     1801 cctgaaaaaa tcaatataaa aagaaaaaca acctttacct ttttcatata ccttccctgt
     1861 atttaaaaac cccggtattc atccctgaac accggaagtc tgtatttcaa atcatccttg
     1921 ataacttttg cttaccagtt accatcccgg taaacaaagg atacaaagta atcaaattta
     1981 ctaatatcga ttacattttt aaagtgtggc tgtaatatcg aaacccatga tttttcaaca
     2041 tcaaaccaac cattattaat attatccttt aaaggcatcc ccagactttc aatgacagaa
     2101 ccatcatcac cgaagtcttt tgagtattca ccacctttaa actcttcggt tttcttatca
     2161 aaccaatgaa tatgtaattt aagccccata tttacctctt aaagatattt tttaatgttt
     2221 cgttttggat ccggcccttt aacctgctta cccgtttttg gatcgaatgc cccgaggtgt
     2281 tcgccatcac tggcgcggta cccttcaagc tcaccgtgct gggaatccca ttcataaatt
     2341 ttacgctttt tatcaccata ccatcgggcc cgcttaccac caccaccttg tttaggggtt
     2401 ttaggtttac cctctttcaa ctctcccaac cccttaatat cttcagtctt gggatcagga
     2461 aaataatcat ggccgtaatc tttagttccc ttacgaggtt ttttcttttc ctcatttaat
     2521 ttattttctg catccttcgc ttttttctcc ttttgtttcc ggctctccat cgccccggaa
     2581 agcgcagcct tatccctgga taaggattca ttcatttcgt ttaaagatgt ctgggcagct
     2641 tttaatttct cattctgatt ctgaatatcc ttatcaagat agcgaacctg atcctgccac
     2701 ccggagccat attcatgagg cgggttataa gtcacaaaat catctttatt tgcctctgcc
     2761 tcttttacct ttttatcggc ttcggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2821 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2881 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2941 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3001 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3061 atgataccgc gagacccacg ctcaccggct ccagatttat cagggcactg ttgcaaagtt
     3121 agcgatgagg cagccttttg tcttattcaa aggccttaca tttcaaaaac tctgcttacc
     3181 aggcgcattt cgcccagggg atcaccataa taaaatgctg aggcctggcc tttgcgtagt
     3241 gcacgcatca cctcaatacc tttgatggtg gcgtaagccg tcttcatgga tttaaatccc
     3301 agcgtggcgc cgattatccg tttcagtttg ccatgatcgc attcaatcac gttgttccgg
     3361 tacttaatct gtcggtgttc aacgtcagac gggcaccggc cttcgcgttt gagcagagca
     3421 agcgcgcgac cataggcggg cgctttatcc gtgttgatga atcgcgggat ctgccacttc
     3481 ttcacgttgt tgaggatttt acccagaaac cggtatgcag ctttgctgtt acgacgggag
     3541 gagagataaa aatcgacagt gcggccccgg ctgtcgacgg cccggtacag atacgcccag
     3601 cggccattga ccttcacgta ggtttcatcc atgtgccacg ggcaaagatc ggaagggtta
     3661 cgccagtacc agcgcagccg tttttccatt tcaggcgcat aacgctgaac ccagcggtaa
     3721 atcgtggagt gatcgacatt cactccgcgt tcagccagca tctcctgcag ctcacggtaa
     3781 ctgatgccgt atttgcagta ccagcgtacg gcccaccgaa tgatgtcacg ctgaaaatgc
     3841 cggcctttga atgggttcat gtgcagctcc atcagcaaaa ggggatgata agtttatcac
     3901 caccgactat ttgcaacagt gccgtttcca aaaggtcgtt gatcaaagct cgccgcgttg
     3961 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
     4021 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
     4081 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
     4141 tgatgtttaa cgtttgacat gaggggcggc caagggcgcc agcccttgga cgtccccctc
     4201 gatggaaggg ttaggcaaca ctgcgtgttc gctcgaatgc ctggcgtgtt tgaaccatgt
     4261 acacggctgg accatctggg gtggttacgg taccttgcct ctcaaacccc gctttctcgt
     4321 agcatcggat cgctcgcaag ttgctcggcg acgggtccgt ttggatcttg gtgacctcgg
     4381 gatcattgaa cagcaactca accagagctc gaaccagctt ggttcccaag cctttgccca
     4441 gttgtgatgc attcgccagt aactggtcta ttccgcgtac tcctggatcg gtttcttctt
     4501 cccaccatcc gtccccgctt ccaagagcaa cgtacgactg ggcataccca atcggctctc
     4561 cattcagcat tgcaatgtat ggagtgacgg actcttgcgc taaaacgctt ggcaagtact
     4621 gttcctgtac gtcagcaagt gtcgggcgtg cttcttctcc gccccaccac tcgacgatat
     4681 gagatcgatt tagccactca tagagcatcg caaggtcatg ctcagtcatg aggcgcagtg
     4741 tgacggaatc gttgctgttg gtcacgatgc tgtactttgt gatgcctaac tttgtttgga
     4801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4921 gcacttttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     4981 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattttat aaaccgtgga
     5041 gcgggcaata ctgagctgat gagcaatttc cgttgcacca gtgcccttct gatgaagcgt
     5101 cagcacgacg ttcctgtcca cggtacgcct gcggccaaat ttgattcctt tcagctttgc
     5161 ttcctgtcgg ccctcattcg tgcgctctag gatcctccgg cgttcagcct gtgccacagc
     5221 cgacaggatg gtgaccacca tttgccccat atcaccgtcg gtactgatcc cgtcgtcaat
     5281 aaaccgaacc gctacaccct gagcatcaaa ctcttttatc agttggatca tgtcggcggt
     5341 gtcgcggcca agacggtcga gcttcttcac cagaatgaca tcaccttcct ccaccttcat
     5401 cctcagcaaa tccagccctt cccgatctgt tgaactgccg gatgccttgt cggtaaagat
     5461 gcggttagct tttacccctg catctttgag cgctctgatc tgaatatcga gggactgctg
     5521 gctggttgag acccgcgcat aaccaaaaat tcgcataaaa atgtacctta aatcgaatat
     5581 cagacacgat gtgtctatta tgccaaaatg acgatttaat ggacactcaa acgaagccgt
     5641 tttactatgt ctgataattt ataatatttc gaacggttgc agttgtgtta aaaaagccgt
     5701 caggcaggga ggccgatatg cccgttgatt ttttgaccac tgagcaggtt gagagttatg
     5761 gcaggttcac tggcgaaccc gatgaacttc agctggcgcg ttattttcat cttgatgaag
     5821 cggataaaga atttatcggg aaaagccggg gtgatcacaa tcgacttggt attgccctgc
     5881 aaatcgggtg tgtgcgtttt ctgggcactt ttcttactga catgaatcat attccttccg
     5941 gcgtccggca ttttaccgcc agacagctcg ggattcgtga tatcaccgtt cttgcagaat
     6001 acggtcagag ggaaaatacc cgccgtgagc atgcagcgct gatacgtcag cactatcagt
     6061 atcgtgaatt tgcctggccc tggacatttc gccttacccg tcttttatat acccggagct
     6121 ggataagcaa cgaacgtcct ggcctgcttt tcgacctggc gacagggtgg cttatgcaac
     6181 atcgtattat tctccccgga gccaccacgc tgacccggtt gatttcagag gtaagggaaa
     6241 aggcgacgtt gcgcctgtgg aacaaactgg cactgatacc gtcagccgaa cagcgttcac
     6301 agctggagat gctgctgggg ccaactgatt gcagccgcct gtctttactg gaatcactga
     6361 aaaaaggccc tgtgaccatc agtggtccgg cgtttaatga agcaattgaa cgctggaaaa
     6421 ctctgaacga ttttggcctg catgctgaaa acctgagtac actcccggct gtgcgcctga
     6481 aaaatctcgc acgttatgct ggtatgactt cggtgttcaa tattgccagg atgtcaccgc
     6541 agaaaaggat ggcggttctg gttgcctttg tccttgcatg ggaaacgctg gcgctggatg
     6601 atgcactgga cgttctggac gccatgctgg ccgttatcat ccgtgacgcc agaaagattg
     6661 ggcagaaaaa acggctccgc tcgctgaagg atctggataa atctgcattg gcgctcgcca
     6721 gcgcatgttc gtacttgctg aaagaagaaa caccggacga atcgattcgt gctgaggtgt
     6781 tcagctacat ccctaggcaa aagctggctg aaatcatcac gcttgtccgt gaaattgccc
     6841 ggccctcaga cgataatttt catgacgaaa tggtggagca gtacgggcgc gttcgtcgtt
     6901 tcctgcccca tctgctgaat accgttaaat tttcatccgc acctgccggg gttaccactc
     6961 tgaatgcctg tgactacctc agccgggagt tcagctcacg gcggcagttt tttgacgacg
     7021 caccaacgga aatcatcagt cagtcatgga aacggctggt gattaacaag gaaaaacata
     7081 tcacccgaag gggatacacg ctctgctttc tcagtaaact gcaggatagt ctgagacgga
     7141 gggatgtcta cgttaccggc agtaaccggt ggggagatcc tcgtgcaaga ttactacagg
     7201 gtgctgactg gcaggcaaat cggattaagg tttatcgttc tttggggcac ccgacagacc
     7261 cgcaggaagc aataaaatct ctgggccatc agcttgatag tcgttacaga caggttgctg
     7321 cacgtcttgg cgaaaatgag gctgtcgaac tcgatgtttc tggcccgaag ccccggttga
     7381 caatttctcc cctcgccagt cttgatgagc cggacagtct gaaacgactg agcaaaatga
     7441 tcagtgatct gctccctccg gtggatttaa cggagttgct gctcgaaatt aacgcccata
     7501 ccggatttgc tgatgagttt ttccatgcca gtgaagccag tgccagagtt gatgatctgc
     7561 ccgtcagcat cagcgccgtg ctgatggctg aagcctgcaa tatcggtctg gaaccactga
     7621 tcagatcaaa tgttcctgca ctgacccgac accggctgaa ctggacaaaa gcgaactatc
     7681 tgcgggctga aactatcacc agcgctaatg ccagactggt tgattttcag gcaacgctgc
     7741 cactggcaca gatatggggt ggaggagaag tggcatctgc agatggaatg cgctttgtta
     7801 cgccagtcag aacaatcaat gccggaccga accgcaaata ctttggtaat aacagaggga
     7861 tcacctggta caactttgtg tccgatcagt attccggctt tcatggcatc gttataccgg
     7921 ggacgctgag ggactctatc tttgtgctgg aaggccttct ggaacaggag accgggctga
     7981 atccaaccga aattatgacc gatacggcag gtgccagcga tcttgtcttt ggccttttct
     8041 ggctgctggg ataccagttt tctccacgcc tggctgatgc cggtgcttcg gttttctggc
     8101 gaatggacca tgatgccgac tatggcgtgc tgaatgatat tgccagaggg caatcagatc
     8161 cccgaaaaat agtccttcag tgggacgaaa tgatccggac cgcaggctcc ctgaagctgg
     8221 gcaaagtaca ggcctcagtg ctggtccgtt cattgctgaa aagtgaacgt ccctccggac
     8281 tgactcaggc aatcattgaa gtggggcgca tcaacaaaac gctgtatctg cttaattata
     8341 ttgatgatga agattaccgc cggcgcattc tgacccagct taatcgggga gaaagccgtc
     8401 atgcagttgc cagagccatc tgtcacggtc aaaaaggtga gataagaaaa cgatataccg
     8461 acggtcagga agatcagttg ggagctctgg ggctggtcac taacgccgtc gtgttatgga
     8521 acactattta tatgcaggca gctctggatc atctccgggc gcagggtgaa acactgaatg
     8581 atgaagatat cgcacgcctc tccccgcttt gccacggaca tatcaatatg ctcggccatt
     8641 attccttcac gctggcagaa ctggtgacca aaggtcatct gagaccatta aaagaggcgt
     8701 cagaggcaga aaacgttgct taacgtgagt tttcgttcca ctgagcgtca gacccctttt
     8761 gatcccaatt tttccaccca gaaagctgac acaacacgaa tgatggtgcc ttttttccgg
     8821 cagttctggg atatgggcgc aaaggataaa gcgaccggaa aatcacggag tgatgtgcag
     8881 caacgcattc agcagtttca cagtcaggaa tttttaaact cactccaagg cacaacacaa
     8941 tttgcgggta ccgattaccg cagcaaagac ctcacaccga aaaaatcaag gctactggct
     9001 gacaccattt cagctgttta tctcgatggc tatgaaggca gacagtaagt ggatttacca
     9061 taacgcgtca ttacgcgcac cgctcagacg ggctcagggc gtttccggcg gctacgggat
     9121 aaaacaggca acaacccccc cggaaaactg ccggaaacgc gcccgataaa ttttatccta
     9181 atgcatgcct atgcacgcaa ctgcatggca tatgttttga cgtaaaatgg atgccgtaag
     9241 ccctttttta ccgactttct gcttcatcag cctgactgac acgaccatat tctttcagac
     9301 cgccccctgc taaacttcct tcatattgcc gcattaaccc gggaggctcc gcaatgagca
     9361 acactacaca ctacgaaaac gccaactttc ttcgggaact ggctgaaagc ctgccccgca
     9421 tactgccgga aggtggcccg gacaaagctg cgctgttgca gcgactggct aacgaagaac
     9481 tggcccaggc agagtatgaa gatcaggttc gcgccaaagt gaccgccgcc cgtgctgaca
     9541 cgcgtccggg tatgaccacg gaacaactcc gccagcgcct gcatggccgt tatcaggaac
     9601 tgcgcgatgc cgtatgacgt ggaatggaag cagggagcca tcgaggatgt aacgacgctg
     9661 tttgactata tcgcggaaaa ttcatccctg tgggatgcgc agaacgttac cgaccgggtt
     9721 ctcgctgcgg cggacaaact ggccgacttc ccgcgactct atgaagtgga ctcacgctat
     9781 ggcgaggacg ttcgccggat cagtctgatg ggacagcatg tcctctatga tgttgatgac
     9841 cagaccagaa aagtccgggt tctggccgtg gtcggccagc gccagaatcc ccatattgtt
     9901 ctctaataac cggtgacggg gacgcaattc attgcgtggc cgtcgccggt tggacaagca
     9961 cagcgcgtca gcgttttccc cacggccacc accggcgcgg tgttccggcc ggggtctctg
    10021 ctgtctccgg tttgtgttcc agcagtctca tggactgcat ttcctgccgt agcccggcca
    10081 cctcctccct gagtgctttt atctccgcga tcaacgcgtc ggaggccatt ttttcagtct
    10141 cacccgacag tctcaactcg tcccgctcgt gagtctcatc ctgtcttaac tcaccgtaca
    10201 cccggataag ctcgctggta tccaccaccc ggccgccatc agcttccgta cggtatgata
    10261 cgcggccttt cgccatatcc cgatacaggg aacttcgggc tttacccgtc agttccgtcg
    10321 cctgtctcac cgtcacccac gccattgtct caccccgcta tttttgagac agtatacctg
    10381 acaggcgcac accatgcccg gaaggaaaga atcatgacaa aacaggaaaa aacagccctc
    10441 aatatggcta aatttattaa ggagcagtcg ctgctattgc tcgataaact gaatgagctg
    10501 gatatggata ccccggccga catgtgcgag cggctacacg aggatgcgga aacactctgg
    10561 caggtgatgc gggataagct cggggaggaa taacagcacc ggcatggccg gtgctggcat
    10621 ttattttttg ccgaacggcc accaccggcg ggcaggtttg tcctccggca gttcaggtac
    10681 cggcacgtta aaatagcggc tgttatattc ccgcatcagc tccgctttca gcttctcgtt
    10741 caccgcgctt tccccgtgcc gctcactcac cacgtcaatc atcgcgttaa tctgctccgg
    10801 tgagcacttc ccggcatcaa ccacctgcac accgttctcg atggcgaacc gctccacctt
    10861 gcgatagagg taatcacaca gcttcattcc tgtgggtttg ttccgttccc attgcggatt
    10921 tttaccagtg aaaaagtacg tggctttttc gtcgctgttg agccgctcgt catacatccc
    10981 gatggatttc atcatcgcca gatgctgggc ccggtcgaca aaacagaaaa catacgcccc
    11041 ggtctgccag gcgagcacca ccatatcttc cagatgctcg ctggtcggca tgatgcagcg
    11101 ctcgccgttc cggtcctccg cgtcttcaat aaataccatg tacagcgcca taatccctca
    11161 ctccgttgtc ataaatttaa agcacgcaaa caaccgttca cgatccagct ctgccgtttt
    11221 gcgggcctcg ccggtctgtg ggttcacgat ttcatccacc gacaggattg cccgctgcac
    11281 cctgacctgc gtcgcccggt ccgggcagat gtaccagatt tcatcgatgc cgttagcttt
    11341 gcggttgaac agatacccgg acaccacttc ctgataccgg cgacggctct tgacggtgcg
    11401 ctccacctca aacgccaccg ttttaccgtc cagctccgcc agggcgtccg gcactttcat
    11461 gcctttaaga ctcagacggt gcagaatgcg ccagccggtg gcccctctgg cttccatatc
    11521 aaggcgcagg gattgcacgg ccagccggtg cggcagatgt gctgccgaca cccttgacgg
    11581 ctgaaacgga ataacatccg ccagcggttc gtcctcgtcg aacgagaacg ccacgccatg
    11641 cggcgtcagc ccccacacgc tgacctgacg gccgtccacc ggatatttgg tccggaccag
    11701 cagccctttc gccaccaggc tgtcgaggaa gcggtagacc ggctgcaccg ccgcaatccc
    11761 cagccgccgc gcaaccagct ccgccgtgga acacagctct tcccgcaacc agcgcagggt
    11821 cagccgggtt ttctccgcgt tccgcgcctg tcgtttcgcg tggctgacgt tgtacagctc
    11881 cagcgccatc agtaaatccc cgcaaaatca tcatccgctt tcggcccgcc gccgtcggcc
    11941 gggggccgga actccgccag gggatcggtt ttcgccagca ccgggaaacg ctgcaacggg
    12001 atttcccggc gctcaacccg cagcggactg ctgaacgcca tccgggccac acccagcccg
    12061 gtcatgaccg cgaacccgtc cggcaggtgt tgcagggtgt tcacgtcaaa cagcggccgc
    12121 gcctccttct ggatgaagct gtccccgctg atatgctcca cgttccccgc ctcccggctg
    12181 gcacggcggc gctccacgtc caccctgatt tccccggtct gccccgccgc ccactgcgcg
    12241 gactccaggc tggccgcccg gtaaaaccag cggatcggcg tgttatccag taccgtggtt
    12301 ttcacaaatt cagcgggcag atcctgcccg cactgcccga aatcgcccag cgactggtgc
    12361 gcgagcaaca gattgcagtc ccggctgcgc agcgtcccca gtgcgttcag tacatacttc
    12421 gacagcagat atttaatttc atcgagcatc acgctgacgt gtggccactc ccggaactcg
    12481 tcccgcgcaa tgatgatttg cgcgcagcgg gcaaacagca ttttttgcac acggatcacc
    12541 gcctcgtcgt ccatcgagcc gataacgtac aggcagccgc cgccgttgat aatccccgcc
    12601 acatcgatgc cactgtccgt ctgcaggacg ctcaggtcag cgacgttttc cagcctagtg
    12661 atcaacccct tcacgtcctt tttcagggcc tccggcagcg cgtacgccgc cgccagcacc
    12721 tgctgaatgt ttgccccctg cggaaactgc tccgcaatca gcttcgccgc tttctgctcg
    12781 ctgatgcggt agtggtccgc cacgtcgccg gtctcgccca ggttaagccc ggcaatcagc
    12841 aggtttttca gcgcgtactg gtcgagatcg cggaacagat tcagctgcgc cggtttcccc
    12901 ggccgcagat ccagcagggt gaagtccgga cagtgcgccc ggaagacgtg cggcgcccat
    12961 gcgtcccctt tggggtcgaa gtacaccacc gcgtccccga aatcgcgtac gcagcgaatg
    13021 agcgcgttgg tggccatcac gctcttgccg ctcccgggca ggccgaggat ctgaatgttg
    13081 gtcttgcgcc aggtatcacg cggaaccagc accggcttgc cctgctcatc cttacccaga
    13141 aacaccgcct gctcctgccg ggcctgctcc gaggaaaaat cggccagcgc attaaccggt
    13201 accggctccc cgaaatgcag gtggcgcacg tcggtcatct ctcctgtccg ctggctgctg
    13261 cgccggctct cctgattcag tttcgcgttc accgcctcgt cagcagccgc aaaatgaccg
    13321 tcaagcagca ggaacgccag cagccgcgcc aacacaatcc cgacagccag cccgcccgcg
    13381 caccaggcaa tcccgaacag gttagccggc tgcagcaggt aataatctgc gtttgcgtag
    13441 aggtccggac gcaggacggt ttctccccag ctctcgataa acacggcgac aaccagcccc
    13501 cccagaaggg ccagaccggc cgcgccggtc agccagtacg cgtagttcca gacatgtcga
    13561 cgtgccctga ccatccgccc gggcgaatcc agcgaggcca gcaccgaaaa tggcgctggc
    13621 cgtttattat cgcgct
//
