LOCUS       OW967297               13636 bp    DNA     circular BCT 22-MAY-2022
DEFINITION  Klebsiella pneumoniae isolate 512 genome assembly, plasmid: P3.
ACCESSION   OW967297
VERSION     OW967297.1
DBLINK      BioProject: PRJEB42440
            BioSample: SAMEA8065856
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="512"
                     /isolation_source="Hospital Reina Sofia (Cordoba)"
                     /db_xref="taxon:573"
                     /plasmid="P3"
     gene            complement(390..>722)
                     /locus_tag="AI2814V1_5327"
     mRNA            complement(390..722)
                     /locus_tag="AI2814V1_5327"
                     /note="AI2814v1_5327.mrna"
     CDS             complement(390..>722)
                     /locus_tag="AI2814V1_5327"
                     /note="short variant; AI2814v1_5327.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH5343056.1"
                     /translation="LLNVSFRSTERQTPFDPNFSTQKADTTRMMVPFFRQFWDMGAKDK
                     ATGKSRSDVQQRIQQFHSQEFLNSLQGTTQFAGTDYRSKDLTPKKSRLLADTISAVYLD
                     GYEGRQ"
                     /name="hypothetical protein"
     exon            complement(390..722)
                     /locus_tag="AI2814V1_5327"
                     /note="AI2814v1_5327.mrna"
                     /number=1
     gene            complement(715..3720)
                     /locus_tag="AI2814V1_5328"
     mRNA            complement(715..3720)
                     /locus_tag="AI2814V1_5328"
                     /note="AI2814v1_5328.mrna"
     CDS             complement(715..3720)
                     /locus_tag="AI2814V1_5328"
                     /note="long variant; AI2814v1_5328.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH5343185.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="hypothetical protein"
     exon            complement(715..3720)
                     /locus_tag="AI2814V1_5328"
                     /note="AI2814v1_5328.mrna"
                     /number=1
     gene            3882..4439
                     /locus_tag="AI2814V1_5329"
     mRNA            3882..4439
                     /locus_tag="AI2814V1_5329"
                     /note="AI2814v1_5329.mrna"
     CDS             3882..4439
                     /locus_tag="AI2814V1_5329"
                     /note="long variant; AI2814v1_5329.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Transposon Tn3 resolvase"
                     /protein_id="CAH5343312.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="Transposon Tn3 resolvase"
     exon            3882..4439
                     /locus_tag="AI2814V1_5329"
                     /note="AI2814v1_5329.mrna"
                     /number=1
     gene            4622..5227
                     /locus_tag="AI2814V1_5330"
     mRNA            4622..5227
                     /locus_tag="AI2814V1_5330"
                     /note="AI2814v1_5330.mrna"
     CDS             4622..5227
                     /locus_tag="AI2814V1_5330"
                     /note="long variant; AI2814v1_5330.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside N(6')-acetyltransferase type 1"
                     /protein_id="CAH5343315.1"
                     /translation="MSIQHFQTKLGITKYSIVTNSNDSVTLRLMTEHDLAMLYEWLNRS
                     HIVEWWGGEEARPTLADVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGW
                     WEEETDPGVRGIDQLLANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAI
                     RCYEKAGFERQGTVTTPDGPAVYMVQTRQAFERTRSVA"
                     /name="Aminoglycoside N(6')-acetyltransferase type 1"
     exon            4622..5227
                     /locus_tag="AI2814V1_5330"
                     /note="AI2814v1_5330.mrna"
                     /number=1
     gene            <5309..5566
                     /locus_tag="AI2814V1_5331"
     mRNA            5309..5566
                     /locus_tag="AI2814V1_5331"
                     /note="AI2814v1_5331.mrna"
     CDS             <5309..5566
                     /locus_tag="AI2814V1_5331"
                     /note="short variant; AI2814v1_5331.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptomycin 3''-adenylyltransferase"
                     /protein_id="CAH5343449.1"
                     /translation="VIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDID
                     LLVTVTVRLDETTRRALINDLLETALLQIVGGDKLIIPFC"
                     /name="Streptomycin 3''-adenylyltransferase"
     exon            5309..5566
                     /locus_tag="AI2814V1_5331"
                     /note="AI2814v1_5331.mrna"
                     /number=1
     gene            5578..6282
                     /locus_tag="AI2814V1_5332"
     mRNA            5578..6282
                     /locus_tag="AI2814V1_5332"
                     /note="AI2814v1_5332.mrna"
     CDS             5578..6282
                     /locus_tag="AI2814V1_5332"
                     /note="long variant; AI2814v1_5332.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH5343576.1"
                     /translation="MNPFKGRHFQRDIIRWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="hypothetical protein"
     exon            5578..6282
                     /locus_tag="AI2814V1_5332"
                     /note="AI2814v1_5332.mrna"
                     /number=1
     gene            <6703..7239
                     /locus_tag="AI2814V1_5333"
     mRNA            6703..7239
                     /locus_tag="AI2814V1_5333"
                     /note="AI2814v1_5333.mrna"
     CDS             <6703..7239
                     /locus_tag="AI2814V1_5333"
                     /note="short variant; AI2814v1_5333.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Colicin-E3"
                     /protein_id="CAH5343692.1"
                     /translation="VTYNPPHEYGSGWQDQVRYLDKDIQNQNEKLKAAQTSLNEMNESL
                     SRDKAALSGAMESRKQKEKKAKDAENKLNEEKKKPRKGTKDYGHDYFPDPKTEDIKGLG
                     ELKEGKPKTPKQGGGGKRARWYGDKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGK
                     QVKGPDPKRNIKKYL"
                     /name="Colicin-E3"
     exon            6703..7239
                     /locus_tag="AI2814V1_5333"
                     /note="AI2814v1_5333.mrna"
                     /number=1
     gene            7249..7506
                     /locus_tag="AI2814V1_5334"
     mRNA            7249..7506
                     /locus_tag="AI2814V1_5334"
                     /note="AI2814v1_5334.mrna"
     CDS             7249..7506
                     /locus_tag="AI2814V1_5334"
                     /note="long variant; AI2814v1_5334.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Colicin-E3 immunity protein"
                     /protein_id="CAH5343819.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="Colicin-E3 immunity protein"
     exon            7249..7506
                     /locus_tag="AI2814V1_5334"
                     /note="AI2814v1_5334.mrna"
                     /number=1
     gene            8381..8509
                     /locus_tag="AI2814V1_5335"
     mRNA            8381..8509
                     /locus_tag="AI2814V1_5335"
                     /note="AI2814v1_5335.mrna"
     CDS             8381..8509
                     /locus_tag="AI2814V1_5335"
                     /note="long variant; AI2814v1_5335.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH5343952.1"
                     /translation="MEKRHLRGLAVLPHMLVPCLFTESVGNFACVRSARRSLTAGA"
                     /name="hypothetical protein"
     exon            8381..8509
                     /locus_tag="AI2814V1_5335"
                     /note="AI2814v1_5335.mrna"
                     /number=1
     gene            9221..11185
                     /locus_tag="AI2814V1_5336"
     mRNA            9221..11185
                     /locus_tag="AI2814V1_5336"
                     /note="AI2814v1_5336.mrna"
     CDS             9221..11185
                     /locus_tag="AI2814V1_5336"
                     /note="long variant; AI2814v1_5336.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH5344072.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="hypothetical protein"
     exon            9221..11185
                     /locus_tag="AI2814V1_5336"
                     /note="AI2814v1_5336.mrna"
                     /number=1
     gene            11185..11916
                     /locus_tag="AI2814V1_5337"
     mRNA            11185..11916
                     /locus_tag="AI2814V1_5337"
                     /note="AI2814v1_5337.mrna"
     CDS             11185..11916
                     /locus_tag="AI2814V1_5337"
                     /note="long variant; AI2814v1_5337.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH5344203.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="hypothetical protein"
     exon            11185..11916
                     /locus_tag="AI2814V1_5337"
                     /note="AI2814v1_5337.mrna"
                     /number=1
     gene            11923..12453
                     /locus_tag="AI2814V1_5338"
     mRNA            11923..12453
                     /locus_tag="AI2814V1_5338"
                     /note="AI2814v1_5338.mrna"
     CDS             11923..12453
                     /locus_tag="AI2814V1_5338"
                     /note="long variant; AI2814v1_5338.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH5344325.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     exon            11923..12453
                     /locus_tag="AI2814V1_5338"
                     /note="AI2814v1_5338.mrna"
                     /number=1
     gene            complement(12481..12660)
                     /locus_tag="AI2814V1_5339"
     mRNA            complement(12481..12660)
                     /locus_tag="AI2814V1_5339"
                     /note="AI2814v1_5339.mrna"
     CDS             complement(12481..12660)
                     /locus_tag="AI2814V1_5339"
                     /note="long variant; AI2814v1_5339.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Regulatory protein rop"
                     /protein_id="CAH5344450.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Regulatory protein rop"
     exon            complement(12481..12660)
                     /locus_tag="AI2814V1_5339"
                     /note="AI2814v1_5339.mrna"
                     /number=1
     gene            12729..13106
                     /locus_tag="AI2814V1_5340"
     mRNA            12729..13106
                     /locus_tag="AI2814V1_5340"
                     /note="AI2814v1_5340.mrna"
     CDS             12729..13106
                     /locus_tag="AI2814V1_5340"
                     /note="long variant; AI2814v1_5340.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH5344455.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     exon            12729..13106
                     /locus_tag="AI2814V1_5340"
                     /note="AI2814v1_5340.mrna"
                     /number=1
     gene            complement(13168..13467)
                     /locus_tag="AI2814V1_5341"
     mRNA            complement(13168..13467)
                     /locus_tag="AI2814V1_5341"
                     /note="AI2814v1_5341.mrna"
     CDS             complement(13168..13467)
                     /locus_tag="AI2814V1_5341"
                     /note="long variant; AI2814v1_5341.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH5344583.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="hypothetical protein"
     exon            complement(13168..13467)
                     /locus_tag="AI2814V1_5341"
                     /note="AI2814v1_5341.mrna"
                     /number=1
     ARG             4674..5224
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             5310..5566
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             4623..5227
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     Typing          complement(7844..8422)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          11186..11913
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 gccaccttcc ggcagtatgc ggggcaggct ttcagccagt tcccgaagaa agttggcgtt
       61 ttcgtagtgt gtagtgttgc tcattgcgga gcctcccggg ttaatgcggc aatatgaagg
      121 aagtttagca gggggcggtc tgaaagaata tggtcgtgtc agtcaggctg atgaagcaga
      181 aagtcggtaa aaaagggctt acggcatcca ttttacgtca aaacatatgc catgcagttg
      241 cgtgcatagg catgcattag gataaaattt atcgggcgcg tttccggcag ttttccgggg
      301 gggttgttgc ctgttttatc ccgtagccgc cggaaacgcc ctgagcccgt ctgagcggtg
      361 cgcgtaatga cgcgttatgg taaatccact tactgtctgc cttcatagcc atcgagataa
      421 acagctgaaa tggtgtcagc cagtagcctt gattttttcg gtgtgaggtc tttgctgcgg
      481 taatcggtac ccgcaaattg tgttgtgcct tggagtgagt ttaaaaattc ctgactgtga
      541 aactgctgaa tgcgttgctg cacatcactc cgtgattttc cggtcgcttt atcctttgcg
      601 cccatatccc agaactgccg gaaaaaaggc accatcattc gtgttgtgtc agctttctgg
      661 gtggaaaaat tgggatcaaa aggggtctga cgctcagtgg aacgaaaact cacgttaagc
      721 aacgttttct gcctctgacg cctcttttaa tggtctcaga tgacctttgg tcaccagttc
      781 tgccagcgtg aaggaataat ggccgagcat attgatatgt ccgtggcaaa gcggggagag
      841 gcgtgcgata tcttcatcat tcagtgtttc accctgcgcc cggagatgat ccagagctgc
      901 ctgcatataa atagtgttcc ataacacgac ggcgttagtg accagcccca gagctcccaa
      961 ctgatcttcc tgaccgtcgg tatatcgttt tcttatctca cctttttgac cgtgacagat
     1021 ggctctggca actgcatgac ggctttctcc ccgattaagc tgggtcagaa tgcgccggcg
     1081 gtaatcttca tcatcaatat aattaagcag atacagcgtt ttgttgatgc gccccacttc
     1141 aatgattgcc tgagtcagtc cggagggacg ttcacttttc agcaatgaac ggaccagcac
     1201 tgaggcctgt actttgccca gcttcaggga gcctgcggtc cggatcattt cgtcccactg
     1261 aaggactatt tttcggggat ctgattgccc tctggcaata tcattcagca cgccatagtc
     1321 ggcatcatgg tccattcgcc agaaaaccga agcaccggca tcagccaggc gtggagaaaa
     1381 ctggtatccc agcagccaga aaaggccaaa gacaagatcg ctggcacctg ccgtatcggt
     1441 cataatttcg gttggattca gcccggtctc ctgttccaga aggccttcca gcacaaagat
     1501 agagtccctc agcgtccccg gtataacgat gccatgaaag ccggaatact gatcggacac
     1561 aaagttgtac caggtgatcc ctctgttatt accaaagtat ttgcggttcg gtccggcatt
     1621 gattgttctg actggcgtaa caaagcgcat tccatctgca gatgccactt ctcctccacc
     1681 ccatatctgt gccagtggca gcgttgcctg aaaatcaacc agtctggcat tagcgctggt
     1741 gatagtttca gcccgcagat agttcgcttt tgtccagttc agccggtgtc gggtcagtgc
     1801 aggaacattt gatctgatca gtggttccag accgatattg caggcttcag ccatcagcac
     1861 ggcgctgatg ctgacgggca gatcatcaac tctggcactg gcttcactgg catggaaaaa
     1921 ctcatcagca aatccggtat gggcgttaat ttcgagcagc aactccgtta aatccaccgg
     1981 agggagcaga tcactgatca ttttgctcag tcgtttcaga ctgtccggct catcaagact
     2041 ggcgagggga gaaattgtca accggggctt cgggccagaa acatcgagtt cgacagcctc
     2101 attttcgcca agacgtgcag caacctgtct gtaacgacta tcaagctgat ggcccagaga
     2161 ttttattgct tcctgcgggt ctgtcgggtg ccccaaagaa cgataaacct taatccgatt
     2221 tgcctgccag tcagcaccct gtagtaatct tgcacgagga tctccccacc ggttactgcc
     2281 ggtaacgtag acatccctcc gtctcagact atcctgcagt ttactgagaa agcagagcgt
     2341 gtatcccctt cgggtgatat gtttttcctt gttaatcacc agccgtttcc atgactgact
     2401 gatgatttcc gttggtgcgt cgtcaaaaaa ctgccgccgt gagctgaact cccggctgag
     2461 gtagtcacag gcattcagag tggtaacccc ggcaggtgcg gatgaaaatt taacggtatt
     2521 cagcagatgg ggcaggaaac gacgaacgcg cccgtactgc tccaccattt cgtcatgaaa
     2581 attatcgtct gagggccggg caatttcacg gacaagcgtg atgatttcag ccagcttttg
     2641 cctagggatg tagctgaaca cctcagcacg aatcgattcg tccggtgttt cttctttcag
     2701 caagtacgaa catgcgctgg cgagcgccaa tgcagattta tccagatcct tcagcgagcg
     2761 gagccgtttt ttctgcccaa tctttctggc gtcacggatg ataacggcca gcatggcgtc
     2821 cagaacgtcc agtgcatcat ccagcgccag cgtttcccat gcaaggacaa aggcaaccag
     2881 aaccgccatc cttttctgcg gtgacatcct ggcaatattg aacaccgaag tcataccagc
     2941 ataacgtgcg agatttttca ggcgcacagc cgggagtgta ctcaggtttt cagcatgcag
     3001 gccaaaatcg ttcagagttt tccagcgttc aattgcttca ttaaacgccg gaccactgat
     3061 ggtcacaggg ccttttttca gtgattccag taaagacagg cggctgcaat cagttggccc
     3121 cagcagcatc tccagctgtg aacgctgttc ggctgacggt atcagtgcca gtttgttcca
     3181 caggcgcaac gtcgcctttt cccttacctc tgaaatcaac cgggtcagcg tggtggctcc
     3241 ggggagaata atacgatgtt gcataagcca ccctgtcgcc aggtcgaaaa gcaggccagg
     3301 acgttcgttg cttatccagc tccgggtata taaaagacgg gtaaggcgaa atgtccaggg
     3361 ccaggcaaat tcacgatact gatagtgctg acgtatcagc gctgcatgct cacggcgggt
     3421 attttccctc tgaccgtatt ctgcaagaac ggtgatatca cgaatcccga gctgtctggc
     3481 ggtaaaatgc cggacgccgg aaggaatatg attcatgtca gtaagaaaag tgcccagaaa
     3541 acgcacacac ccgatttgca gggcaatacc aagtcgattg tgatcacccc ggcttttccc
     3601 gataaattct ttatccgctt catcaagatg aaaataacgc gccagctgaa gttcatcggg
     3661 ttcgccagtg aacctgccat aactctcaac ctgctcagtg gtcaaaaaat caacgggcat
     3721 atcggcctcc ctgcctgacg gcttttttaa cacaactgca accgttcgaa atattataaa
     3781 ttatcagaca tagtaaaacg gcttcgtttg agtgtccatt aaatcgtcat tttggcataa
     3841 tagacacatc gtgtctgata ttcgatttaa ggtacatttt tatgcgaatt tttggttatg
     3901 cgcgggtctc aaccagccag cagtccctcg atattcagat cagagcgctc aaagatgcag
     3961 gggtaaaagc taaccgcatc tttaccgaca aggcatccgg cagttcaaca gatcgggaag
     4021 ggctggattt gctgaggatg aaggtggagg aaggtgatgt cattctggtg aagaagctcg
     4081 accgtcttgg ccgcgacacc gccgacatga tccaactgat aaaagagttt gatgctcagg
     4141 gtgtagcggt tcggtttatt gacgacggga tcagtaccga cggtgatatg gggcaaatgg
     4201 tggtcaccat cctgtcggct gtggcacagg ctgaacgccg gaggatccta gagcgcacga
     4261 atgagggccg acaggaagca aagctgaaag gaatcaaatt tggccgcagg cgtaccgtgg
     4321 acaggaacgt cgtgctgacg cttcatcaga agggcactgg tgcaacggaa attgctcatc
     4381 agctcagtat tgcccgctcc acggtttata aaattcttga agacgaaagg gcctcgtgat
     4441 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4501 ttttcgggga aaagtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4561 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4621 tatgagtatt caacatttcc aaacaaagtt aggcatcaca aagtacagca tcgtgaccaa
     4681 cagcaacgat tccgtcacac tgcgcctcat gactgagcat gaccttgcga tgctctatga
     4741 gtggctaaat cgatctcata tcgtcgagtg gtggggcgga gaagaagcac gcccgacact
     4801 tgctgacgta caggaacagt acttgccaag cgttttagcg caagagtccg tcactccata
     4861 cattgcaatg ctgaatggag agccgattgg gtatgcccag tcgtacgttg ctcttggaag
     4921 cggggacgga tggtgggaag aagaaaccga tccaggagta cgcggaatag accagttact
     4981 ggcgaatgca tcacaactgg gcaaaggctt gggaaccaag ctggttcgag ctctggttga
     5041 gttgctgttc aatgatcccg aggtcaccaa gatccaaacg gacccgtcgc cgagcaactt
     5101 gcgagcgatc cgatgctacg agaaagcggg gtttgagagg caaggtaccg taaccacccc
     5161 agatggtcca gccgtgtaca tggttcaaac acgccaggca ttcgagcgaa cacgcagtgt
     5221 tgcctaaccc ttccatcgag ggggacgtcc aagggctggc gcccttggcc gcccctcatg
     5281 tcaaacgtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     5341 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     5401 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     5461 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacggcact
     5521 gttgcaaata gtcggtggtg ataaacttat catccccttt tgctgatgga gctgcacatg
     5581 aacccattca aaggccggca ttttcagcgt gacatcattc ggtgggccgt acgctggtac
     5641 tgcaaatacg gcatcagtta ccgtgagctg caggagatgc tggctgaacg cggagtgaat
     5701 gtcgatcact ccacgattta ccgctgggtt cagcgttatg cgcctgaaat ggaaaaacgg
     5761 ctgcgctggt actggcgtaa cccttccgat ctttgcccgt ggcacatgga tgaaacctac
     5821 gtgaaggtca atggccgctg ggcgtatctg taccgggccg tcgacagccg gggccgcact
     5881 gtcgattttt atctctcctc ccgtcgtaac agcaaagctg cataccggtt tctgggtaaa
     5941 atcctcaaca acgtgaagaa gtggcagatc ccgcgattca tcaacacgga taaagcgccc
     6001 gcctatggtc gcgcgcttgc tctgctcaaa cgcgaaggcc ggtgcccgtc tgacgttgaa
     6061 caccgacaga ttaagtaccg gaacaacgtg attgaatgcg atcatggcaa actgaaacgg
     6121 ataatcggcg ccacgctggg atttaaatcc atgaagacgg cttacgccac catcaaaggt
     6181 attgaggtga tgcgtgcact acgcaaaggc caggcctcag cattttatta tggtgatccc
     6241 ctgggcgaaa tgcgcctggt aagcagagtt tttgaaatgt aaggcctttg aataagacaa
     6301 aaggctgcct catcgctaac tttgcaacag tgccctgata aatctggagc cggtgagcgt
     6361 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6421 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6481 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6541 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6601 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgaagcc
     6661 gataaaaagg taaaagaggc agaggcaaat aaagatgatt ttgtgactta taacccgcct
     6721 catgaatatg gctccgggtg gcaggatcag gttcgctatc ttgataagga tattcagaat
     6781 cagaatgaga aattaaaagc tgcccagaca tctttaaacg aaatgaatga atccttatcc
     6841 agggataagg ctgcgctttc cggggcgatg gagagccgga aacaaaagga gaaaaaagcg
     6901 aaggatgcag aaaataaatt aaatgaggaa aagaaaaaac ctcgtaaggg aactaaagat
     6961 tacggccatg attattttcc tgatcccaag actgaagata ttaaggggtt gggagagttg
     7021 aaagagggta aacctaaaac ccctaaacaa ggtggtggtg gtaagcgggc ccgatggtat
     7081 ggtgataaaa agcgtaaaat ttatgaatgg gattcccagc acggtgagct tgaagggtac
     7141 cgcgccagtg atggcgaaca cctcggggca ttcgatccaa aaacgggtaa gcaggttaaa
     7201 gggccggatc caaaacgaaa cattaaaaaa tatctttaag aggtaaatat ggggcttaaa
     7261 ttacatattc attggtttga taagaaaacc gaagagttta aaggtggtga atactcaaaa
     7321 gacttcggtg atgatggttc tgtcattgaa agtctgggga tgcctttaaa ggataatatt
     7381 aataatggtt ggtttgatgt tgaaaaatca tgggtttcga tattacagcc acactttaaa
     7441 aatgtaatcg atattagtaa atttgattac tttgtatcct ttgtttaccg ggatggtaac
     7501 tggtaagcaa aagttatcaa ggatgatttg aaatacagac ttccggtgtt cagggatgaa
     7561 taccggggtt tttaaataca gggaaggtat atgaaaaagg taaaggttgt ttttcttttt
     7621 atattgattt tttcaggctt tcttctggtc gcatgtcagg caaactatat ccgggatgtt
     7681 cagggtggaa cggtggcacc atcgtcctcc tctgaactga cggggatcgc ggttcagtag
     7741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatcttt tgccctgtaa
     7801 acgaaaaaac cacctgggga ggtggtttga tcgaaggtta agtcagttgg ggaactgctt
     7861 aaccgtggta actggctttc gcagagcaca gcaaccaaat ctgtccttcc agtgtagccg
     7921 gactttggcg cacacttcaa gagcaaccgc gtgtttagct aaacaaatcc tctgcgaact
     7981 cccagttacc aatggctgct gccagtggcg ttttgcgtgc ttttccgggt tggactcaag
     8041 tgaacagtta ccggataagg cgcagcagtc gggctgaacg gggggttctt gcttacagcc
     8101 cagcttggag cgaacgacct acaccgagcc gagataccag agtgtgagct atgagaaagc
     8161 gccacacttc ccgcaaggga gaaaggcgga acaggtatcc gggaaacggc agggttggaa
     8221 caggagagcg caagagggag cgattcatcg gaaacggtga tgatctttaa gtcctgtcgg
     8281 gtttcgccac tcctgactga ttcatggttg agccaccggc tcccacagat gcaccgaaaa
     8341 agcgtctgtt tatgtgaact cagtcaggag ggcggagcct atggaaaaac gccacctgcg
     8401 tggccttgcc gttttgcctc acatgttagt cccctgctta ttcacggaat ctgtgggtaa
     8461 ctttgcatgt gtccgcagcg cccgccgcag tctcacggcc ggagcgtagc gaccgagtga
     8521 gcgaggaggc gcaatttccc cggcacctac aagacggaca ccggacgcgc tgatgtggca
     8581 tattgtcccc tgattaagtc tttatattcc ctgataataa attaccagga taggtgaatc
     8641 atatcggttt ttcgctgaac attttacgtt aacctatctc tgttgctttt ttctccttac
     8701 ggagaaatac aggtcatccc cgcgagggcg tggctgacct cagggggata tcccctgctt
     8761 acgctggctg gacgccaccg acgcacggtc tctccccctc tttctggctc cgtccggctg
     8821 gacgccggcc acgccagaaa ttcacccggc ccccgtcagg ctcacgcctg accacccaca
     8881 aaaatccacg gtgctggacg cacctggcga agcagcaaag ctgctcctga ttttgcgggt
     8941 ggtcagccgc tcgctatggc gaactctgac gggggccgga acaccggaaa agaccgcact
     9001 ggggtgccgc cttttcctct cacacgctgg ggcgtgctcg ttgttcctcc ggggctgcgc
     9061 cttttccgca tggggcggaa agaggctggc ctccgtggtg aaagctgaac gcaatcacca
     9121 cggacgtgac cgccgccgcc atgccttggg gcatgtccgc ggcttgtgca gcgggggctg
     9181 cacatcacgc cggcaaacca tcgtctgaag gagagagaac atgtttaata cggattcgct
     9241 tgcctggaag tggcacattc cggagctggc ccggcagatt tatccgtggg tcagctggct
     9301 gtaccaggag tattacggtc tggccggggc tttcctagtt atcggcctgt ttttcggtct
     9361 ggctgaactt tgggccggac ggatgcgttt tgtggggctg agtcataagg agcgacggga
     9421 gtatgccgat cgctataagc gcgataataa acggccagcg ccattttcgg tgctggcctc
     9481 gctggattcg cccgggcgga tggtcagggc acgtcgacat gtctggaact acgcgtactg
     9541 gctgaccggc gcggccggtc tggcccttct gggggggctg gttgtcgccg tgtttatcga
     9601 gagctgggga gaaaccgtcc tgcgtccgga cctctacgca aacgcagatt attacctgct
     9661 gcagccggct aacctgttcg ggattgcctg gtgcgcgggc gggctggctg tcgggattgt
     9721 gttggcgcgg ctgctggcgt tcctgctgct tgacggtcat tttgcggctg ctgacgaggc
     9781 ggtgaacgcg aaactgaatc aggagagccg gcgcagcagc cagcggacag gagagatgac
     9841 cgacgtgcgc cacctgcatt tcggggagcc ggtaccggtt aatgcgctgg ccgatttttc
     9901 ctcggagcag gcccggcagg agcaggcggt gtttctgggt aaggatgagc agggcaagcc
     9961 ggtgctggtt ccgcgtgata cctggcgcaa gaccaacatt cagatcctcg gcctgcccgg
    10021 gagcggcaag agcgtgatgg ccaccaacgc gctcattcgc tgcgtacgcg atttcgggga
    10081 cgcggtggtg tacttcgacc ccaaagggga cgcatgggcg ccgcacgtct tccgggcgca
    10141 ctgtccggac ttcaccctgc tggatctgcg gccggggaaa ccggcgcagc tgaatctgtt
    10201 ccgcgatctc gaccagtacg cgctgaaaaa cctgctgatt gccgggctta acctgggcga
    10261 gaccggcgac gtggcggacc actaccgcat cagcgagcag aaagcggcga agctgattgc
    10321 ggagcagttt ccgcaggggg caaacattca gcaggtgctg gcggcggcgt acgcgctgcc
    10381 ggaggccctg aaaaaggacg tgaaggggtt gatcactagg ctggaaaacg tcgctgacct
    10441 gagcgtcctg cagacggaca gtggcatcga tgtggcgggg attatcaacg gcggcggctg
    10501 cctgtacgtt atcggctcga tggacgacga ggcggtgatc cgtgtgcaaa aaatgctgtt
    10561 tgcccgctgc gcgcaaatca tcattgcgcg ggacgagttc cgggagtggc cacacgtcag
    10621 cgtgatgctc gatgaaatta aatatctgct gtcgaagtat gtactgaacg cactggggac
    10681 gctgcgcagc cgggactgca atctgttgct cgcgcaccag tcgctgggcg atttcgggca
    10741 gtgcgggcag gatctgcccg ctgaatttgt gaaaaccacg gtactggata acacgccgat
    10801 ccgctggttt taccgggcgg ccagcctgga gtccgcgcag tgggcggcgg ggcagaccgg
    10861 ggaaatcagg gtggacgtgg agcgccgccg tgccagccgg gaggcgggga acgtggagca
    10921 tatcagcggg gacagcttca tccagaagga ggcgcggccg ctgtttgacg tgaacaccct
    10981 gcaacacctg ccggacgggt tcgcggtcat gaccgggctg ggtgtggccc ggatggcgtt
    11041 cagcagtccg ctgcgggttg agcgccggga aatcccgttg cagcgtttcc cggtgctggc
    11101 gaaaaccgat cccctggcgg agttccggcc cccggccgac ggcggcgggc cgaaagcgga
    11161 tgatgatttt gcggggattt actgatggcg ctggagctgt acaacgtcag ccacgcgaaa
    11221 cgacaggcgc ggaacgcgga gaaaacccgg ctgaccctgc gctggttgcg ggaagagctg
    11281 tgttccacgg cggagctggt tgcgcggcgg ctggggattg cggcggtgca gccggtctac
    11341 cgcttcctcg acagcctggt ggcgaaaggg ctgctggtcc ggaccaaata tccggtggac
    11401 ggccgtcagg tcagcgtgtg ggggctgacg ccgcatggcg tggcgttctc gttcgacgag
    11461 gacgaaccgc tggcggatgt tattccgttt cagccgtcaa gggtgtcggc agcacatctg
    11521 ccgcaccggc tggccgtgca atccctgcgc cttgatatgg aagccagagg ggccaccggc
    11581 tggcgcattc tgcaccgtct gagtcttaaa ggcatgaaag tgccggacgc cctggcggag
    11641 ctggacggta aaacggtggc gtttgaggtg gagcgcaccg tcaagagccg tcgccggtat
    11701 caggaagtgg tgtccgggta tctgttcaac cgcaaagcta acggcatcga tgaaatctgg
    11761 tacatctgcc cggaccgggc gacgcaggtc agggtgcagc gggcaatcct gtcggtggat
    11821 gaaatcgtga acccacagac cggcgaggcc cgcaaaacgg cagagctgga tcgtgaacgg
    11881 ttgtttgcgt gctttaaatt tatgacaacg gagtgaggga ttatggcgct gtacatggta
    11941 tttattgaag acgcggagga ccggaacggc gagcgctgca tcatgccgac cagcgagcat
    12001 ctggaagata tggtggtgct cgcctggcag accggggcgt atgttttctg ttttgtcgac
    12061 cgggcccagc atctggcgat gatgaaatcc atcgggatgt atgacgagcg gctcaacagc
    12121 gacgaaaaag ccacgtactt tttcactggt aaaaatccgc aatgggaacg gaacaaaccc
    12181 acaggaatga agctgtgtga ttacctctat cgcaaggtgg agcggttcgc catcgagaac
    12241 ggtgtgcagg tggttgatgc cgggaagtgc tcaccggagc agattaacgc gatgattgac
    12301 gtggtgagtg agcggcacgg ggaaagcgcg gtgaacgaga agctgaaagc ggagctgatg
    12361 cgggaatata acagccgcta ttttaacgtg ccggtacctg aactgccgga ggacaaacct
    12421 gcccgccggt ggtggccgtt cggcaaaaaa taaatgccag caccggccat gccggtgctg
    12481 ttattcctcc ccgagcttat cccgcatcac ctgccagagt gtttccgcat cctcgtgtag
    12541 ccgctcgcac atgtcggccg gggtatccat atccagctca ttcagtttat cgagcaatag
    12601 cagcgactgc tccttaataa atttagccat attgagggct gttttttcct gttttgtcat
    12661 gattctttcc ttccgggcat ggtgtgcgcc tgtcaggtat actgtctcaa aaatagcggg
    12721 gtgagacaat ggcgtgggtg acggtgagac aggcgacgga actgacgggt aaagcccgaa
    12781 gttccctgta tcgggatatg gcgaaaggcc gcgtatcata ccgtacggaa gctgatggcg
    12841 gccgggtggt ggataccagc gagcttatcc gggtgtacgg tgagttaaga caggatgaga
    12901 ctcacgagcg ggacgagttg agactgtcgg gtgagactga aaaaatggcc tccgacgcgt
    12961 tgatcgcgga gataaaagca ctcagggagg aggtggccgg gctacggcag gaaatgcagt
    13021 ccatgagact gctggaacac aaaccggaga cagcagagac cccggccgga acaccgcgcc
    13081 ggtggtggcc gtggggaaaa cgctgacgcg ctgtgcttgt ccaaccggcg acggccacgc
    13141 aatgaattgc gtccccgtca ccggttatta gagaacaata tggggattct ggcgctggcc
    13201 gaccacggcc agaacccgga cttttctggt ctggtcatca acatcataga ggacatgctg
    13261 tcccatcaga ctgatccggc gaacgtcctc gccatagcgt gagtccactt catagagtcg
    13321 cgggaagtcg gccagtttgt ccgccgcagc gagaacccgg tcggtaacgt tctgcgcatc
    13381 ccacagggat gaattttccg cgatatagtc aaacagcgtc gttacatcct cgatggctcc
    13441 ctgcttccat tccacgtcat acggcatcgc gcagttcctg ataacggcca tgcaggcgct
    13501 ggcggagttg ttccgtggtc atacccggac gcgtgtcagc acgggcggcg gtcactttgg
    13561 cgcgaacctg atcttcatac tctgcctggg ccagttcttc gttagccagt cgctgcaaca
    13621 gcgcagcttt gtccgg
//
