LOCUS       OW849126                6134 bp    DNA     circular BCT 17-MAY-2022
DEFINITION  Enterobacter cloacae isolate 515 genome assembly, plasmid: P4.
ACCESSION   OW849126
VERSION     OW849126.1
DBLINK      BioProject: PRJEB42440
            BioSample: SAMEA7853285
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /isolate="515"
                     /isolation_source="Complejo Hospitalario de Pontevedra
                     (Pontevedra)"
                     /db_xref="taxon:550"
                     /plasmid="P4"
     gene            1..311
                     /locus_tag="AI2719V1_REPA000000343"
     repeat_region   1..311
                     /locus_tag="AI2719V1_REPA000000343"
                     /note="IS5B_IS5_IS5"
                     /rpt_family="IS"
     gene            1..169
                     /locus_tag="AI2719V1_REPA000000345"
     repeat_region   1..169
                     /locus_tag="AI2719V1_REPA000000345"
                     /note="IS5D_IS5_IS5"
                     /rpt_family="IS"
     gene            76..311
                     /locus_tag="AI2719V1_REPA000000344"
     repeat_region   76..311
                     /locus_tag="AI2719V1_REPA000000344"
                     /note="IS5D_IS5_IS5"
                     /rpt_family="IS"
     gene            complement(274..429)
                     /locus_tag="AI2719V1_5069"
     mRNA            complement(274..429)
                     /locus_tag="AI2719V1_5069"
                     /note="AI2719v1_5069.mrna"
     CDS             complement(274..429)
                     /locus_tag="AI2719V1_5069"
                     /note="long variant; AI2719v1_5069.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3893067.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGKVRIS
                     GEILLG"
                     /name="hypothetical protein"
     exon            complement(274..429)
                     /locus_tag="AI2719V1_5069"
                     /note="AI2719v1_5069.mrna"
                     /number=1
     gene            755..1156
                     /locus_tag="AI2719V1_5070"
     mRNA            755..1156
                     /locus_tag="AI2719V1_5070"
                     /note="AI2719v1_5070.mrna"
     CDS             755..1156
                     /locus_tag="AI2719V1_5070"
                     /note="long variant; AI2719v1_5070.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3893165.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNKKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="hypothetical protein"
     exon            755..1156
                     /locus_tag="AI2719V1_5070"
                     /note="AI2719v1_5070.mrna"
                     /number=1
     gene            2298..2600
                     /locus_tag="AI2719V1_5071"
     mRNA            2298..2600
                     /locus_tag="AI2719V1_5071"
                     /note="AI2719v1_5071.mrna"
     CDS             2298..2600
                     /locus_tag="AI2719V1_5071"
                     /note="long variant; AI2719v1_5071.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Antitoxin YefM"
                     /protein_id="CAH3893262.1"
                     /translation="MYLFAYMFYAKPQQMGVNNMHAINFTDARKHFAETMKRVTDDAEP
                     VRVMRRDAPDIVMIDAGEYEALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="Antitoxin YefM"
     exon            2298..2600
                     /locus_tag="AI2719V1_5071"
                     /note="AI2719v1_5071.mrna"
                     /number=1
     gene            <2581..2856
                     /locus_tag="AI2719V1_5072"
     mRNA            2581..2856
                     /locus_tag="AI2719V1_5072"
                     /note="AI2719v1_5072.mrna"
     CDS             <2581..2856
                     /locus_tag="AI2719V1_5072"
                     /note="short variant; AI2719v1_5072.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Toxin YoeB"
                     /protein_id="CAH3893350.1"
                     /translation="LSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Toxin YoeB"
     exon            2581..2856
                     /locus_tag="AI2719V1_5072"
                     /note="AI2719v1_5072.mrna"
                     /number=1
     gene            <3104..4009
                     /locus_tag="AI2719V1_5073"
     mRNA            3104..4009
                     /locus_tag="AI2719V1_5073"
                     /note="AI2719v1_5073.mrna"
     CDS             <3104..4009
                     /locus_tag="AI2719V1_5073"
                     /note="short variant; AI2719v1_5073.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3893445.1"
                     /translation="LHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="hypothetical protein"
     exon            3104..4009
                     /locus_tag="AI2719V1_5073"
                     /note="AI2719v1_5073.mrna"
                     /number=1
     gene            4006..4713
                     /locus_tag="AI2719V1_5074"
     mRNA            4006..4713
                     /locus_tag="AI2719V1_5074"
                     /note="AI2719v1_5074.mrna"
     CDS             4006..4713
                     /locus_tag="AI2719V1_5074"
                     /note="long variant; AI2719v1_5074.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3893545.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     exon            4006..4713
                     /locus_tag="AI2719V1_5074"
                     /note="AI2719v1_5074.mrna"
                     /number=1
     gene            5251..6134
                     /locus_tag="AI2719V1_REPA000000342"
     repeat_region   5251..6134
                     /locus_tag="AI2719V1_REPA000000342"
                     /note="IS5B_IS5_IS5"
                     /rpt_family="IS"
     gene            5251..6133
                     /locus_tag="AI2719V1_REPA000000346"
     repeat_region   5251..6133
                     /locus_tag="AI2719V1_REPA000000346"
                     /note="ISKpn26_IS5_IS5"
                     /rpt_family="IS"
     gene            complement(5595..5951)
                     /locus_tag="AI2719V1_5075"
     mRNA            complement(5595..5951)
                     /locus_tag="AI2719V1_5075"
                     /note="AI2719v1_5075.mrna"
     CDS             complement(5595..5951)
                     /locus_tag="AI2719V1_5075"
                     /note="long variant; AI2719v1_5075.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3893634.1"
                     /translation="MKQITQLIEVMLVGRGGDQAVGQATLGIDTNVGLHAKVPLIAFLG
                     LMHLRIALLLFVLGRAGCLNDGGIDQGALSHHDACFGQPAIDGLEQLAGQLMLLQQVAE
                     IHDGGAVRQGAIQG"
                     /name="hypothetical protein"
     exon            complement(5595..5951)
                     /locus_tag="AI2719V1_5075"
                     /note="AI2719v1_5075.mrna"
                     /number=1
     gene            5721..5872
                     /locus_tag="AI2719V1_REPA000000341"
     repeat_region   5721..5872
                     /locus_tag="AI2719V1_REPA000000341"
                     /note="IS1021_IS5_IS5"
                     /rpt_family="IS"
ORIGIN
        1 gccaaggtgg agcacccatt tcgcatcatc aagcgacagt tcggcttcgt gaaagccaga
       61 tacaaggggt tgctgaaaaa cgataaccaa ctggcgatgt tattcacgct ggccaacctg
      121 tttcgggcgg accaaatgat acgtcagtgg gagagatctc actaaaaact gggaataacg
      181 ccttaaatgg cgaagaaacg gtctaaatag gctgattcaa ggcatttacg ggagaaaaaa
      241 tcggctcaaa catgaagaaa tgaaatggct gagtcagccg agaagaattt ccccgcttat
      301 tcgcaccttc cctaatgata ctgagcgatt cacgaatatt tcatgttcct cattcatttt
      361 ttcaagtgcg tttttgtagt actgatcatt cgcatggtgg tagctaatga ataccttatg
      421 ctttcccatt tttattgtcc tttttttgtt tgtgtgcact ctattttgca cttgaaccta
      481 tatcagataa cagctctctt ttagagagct ctttttttct cagccagcac acatagggtc
      541 ccttcagcaa ggtctcacca tgactggttt agggcgggct cgttgaacgt agtgagcgaa
      601 tgcgcgagga agcggaaagt catttgttgc ctgcattttg cacggtcact cctgtgtttc
      661 atcgcccttc cgaaaaacac atcacttctg gaccagtatg tcgtaagctg gcccctgttt
      721 ccttgagact gaatgtatta aaggatgata actgatgacc agagtgtcta ttagtgaggc
      781 tgcacggctg acgggtaaga gccggacaac gctgcacagg ttaatcaaaa caggtgaact
      841 gtccacttgt agcggtgaac gaaataaaaa aatggtcgat atatcagagc ttttacgtgt
      901 tttcggaccg tttgaccaac cgaagaatga acaccttact cgacaagtac ctgaacagcg
      961 tgatacagcg cctgctgacc agagtgaaca tgtcattcgt caactgaaac agcagattga
     1021 acaccttaac atattgattt tatccaagga ttcgcacatt gacagcctga aacaggccat
     1081 gctccttctt gaaaatcacc aggcaaaatt aactgaaccc ccggcgccgt ggtggaaatt
     1141 ctggaaaaaa tcctgacccc gtcatgcttc agttttcctc tccggccatt ccggggagtt
     1201 cctgaacctt cccgaaaatc gtcagatctc tgaacgctcc tcaccacgaa aaatacccac
     1261 accccacgca aaattaaaat tttgcagatt tgtatttaat atcagcagct tactttactg
     1321 aaatttcgtt ttttaaattt tatttatata gatccgactc tatgacgact cgtcatagag
     1381 tccccggcga gtcgtcatag agtcggtaac gactcaggcc tataaaaaca atgagttaat
     1441 aacgacccga ctctagatcc gactccccgt ttggttgttt tttagacgcg gcgactctgg
     1501 gtacgactct agtgaagagt cgccggtgaa tcgtgtggtg agtcgtccgg cgacttgcca
     1561 taacgtgatt gccggccgtg gacgaaccga ttcctgaacg tgctgaaaac cggttttccg
     1621 gctgcgccgg caacgcttca cggccacgca ataaattgcg tccccgttca ccgttttcgg
     1681 atgggaagaa cgccggaaac aggacttccc ctggctgaat ggtgtcaatt tccgcgctat
     1741 gcacttgtgc gcatactcat gcatgccgta aaaacagagc ctgcgcgttt ctggcgggtt
     1801 ttcgggtggt ttgttgcctt gttttaccgg tttcccgtca gaaacgccct gagggcctct
     1861 caggcggtgc tgataacgga tgttatggta aatcaagtga atggcaggta caaaaaaagc
     1921 cccagagggc ttttagttta gacttctgaa gatattttat ttttcttgga agtggtggtt
     1981 gtttttgctc ggagggaact tatcaccctt gctcacatgg cgatcatatt cagaaccagc
     2041 gtatgagttg tcggtatcat gatattgacc gtaagtacct gtttttggac attcttgccc
     2101 agaccaatgt tttgccattt acttttcctt tgctcgtttt aggagctaag ctcctagcgg
     2161 tacgaaattg tgccgcactt cataaatgta attgcttgaa attatgcttc aatgtattga
     2221 cttgttgttt ttgtggttat tcgattgagt tcacattttg agctgatttg gttgtgggtt
     2281 tttagggaaa tatctttatg tacttatttg cgtacatgtt ctatgctaag ccccaacaaa
     2341 tgggggttaa caacatgcat gcaatcaatt ttactgatgc ccgtaaacat tttgccgaga
     2401 cgatgaagcg cgtcactgat gatgctgaac cggtacgtgt catgcgccgt gatgcgcctg
     2461 atattgttat gatcgatgcg ggagagtatg aggcgctaat cgaaacggtc tatctgttca
     2521 gcaatccggc gaacgcagag catatcaatg agtcaatgaa acaggcggag cgcggcgaat
     2581 ttgtcgaagt ggaatattaa gttcactagg aatggcgctg atgaccttaa ctactggcgt
     2641 gatactgaca aaaagaagtt tgagcgtatc cggcaactgt tgctcagcat cgaggctgat
     2701 ccgctgacag gtataggaaa acccgaacgg ctgcggcacc acaaagatcc ggcactctac
     2761 tcccgcagga ttgatcagag ccaccgtctg gtctattcaa tccagaacgg agaaatcatc
     2821 atctacgcag cccgttatca ctatggtgac aaatagcaaa agtcgatata gcccgcttga
     2881 tgcgagcttt tcatgcaagg cgtacaacgg atgttagggt aagtcgctgt cagatcgaca
     2941 caaaggcaaa gtcggtgtaa cgttagcctc agaaaaaaca aagccccgga aatcatgtca
     3001 tatcttggcg gatcgcatga taaccagggc taggtcgaaa ccttgaaagg atattagcac
     3061 atgaaccgtg ccagacaaca gcccgccata agcgcagggc acattgcata acgccgcact
     3121 cgaacttttc aacgaacgct taccacacaa gccgtacttt tcggacgatc tgcactttgg
     3181 tgtccgtatc gccggtaagg aacgcgctat tctggcgaaa tatatccagt tcaatcagcc
     3241 tcacgccatg ttctggctgg gatttgacgt tgaccgcgcc ggttcctcca tcgactggag
     3301 cgaccgcaac gcgcctgcac cgacgctgac cataactaac cccgaaaacg gacacgcgca
     3361 cctgctgtac gcgctggaaa actcgatacg caccgcgccg gacggcaaaa taaaaccgtt
     3421 acgctacgcc gcctccgtgg agaatgcgct acgtaaaaaa ttagaggctg acgctggata
     3481 ttcggggctc atctgcaaaa atccgaacca caaccactgg aaaatcgccg tgtggcaacc
     3541 gaagctctac actctggact ggctggccga ctttctcgat ctgaacacag ggaatgacaa
     3601 ggaaatcgtt gccgactacg gcttaggtcg taactgcacg ttgttcgaca aaacccgtaa
     3661 gtgggcatat cgcgccatcc gtcagggctg gcctgagtat gagcagtggc tgcaagcctg
     3721 ctacgaacgc gcttgcgcat acaacctgca atttgcacgg cctctggatg aaaatgaggt
     3781 aagagggata gctaagagca ttgctaaatg gacatcaaat aaattttcac ctgaagaatt
     3841 cagtaaattt gttgatatta ctcattcctc tgaaattcaa tcaaagagag ggaaaaagtc
     3901 agggcagtca aggaggaaag gaagcctaga ggaaataaaa ccttgggtag ctatgggaat
     3961 aagcaggcgg aagtattttt atatcaagaa aaatggtgaa ataagatgaa aaccttcgta
     4021 actctttgga ctatagtttt tttagcattt attgccgtga tgtgtactgt tgatatatct
     4081 aattacattc cgtctgttta cacagttttt agtaagaata gacctcttgt aactggcgcc
     4141 ctttctattc ttttgatatc tgtttttatt tggctgatgg tatccctcta cttacttaaa
     4201 aagtattcgg ttaaggttga gaaacttagc cttggtggag tgaatatact tttcaatgag
     4261 agtgctacac tttacaaaaa gagtgtaaaa aatcacctag attcaaaaag agcaattttc
     4321 aaattaagaa aaaacgttga tgcctttgat gaggttattt catcatatta tcagacttac
     4381 caatttattc gtgatgaaat gaaaatacta aaccctaaaa aagatcgtga gatttatgat
     4441 ttcagcaatg agatgctcat ggtgttaaat agatttctaa ctaactacca gaacaactac
     4501 aagcgttggt ataagtacat atcagacaaa gatgaggttg ttgatagggt tagcggtgat
     4561 gtcatgagat ttcatgcaac cagcataaat aaaatacaaa tgcagtatta tgattatgag
     4621 cgtattttaa aaggttttaa agatgttaac cggttctttg ttaatgatgt gaagcctaag
     4681 tttggaatca atacagataa gtggaactgg taaattaaag gagtagccaa ggctactcct
     4741 tggatagtac tatgaattat gcattctcat ttctctcgac atatcatacg ttgctttttc
     4801 tctatcgtta taagtcatgt ctattaattt tctgagtgtt tctgggttgt tttctatttg
     4861 attccagttg accactgata tataagaccc gctcttcatg aagttgtcta ttattcttgc
     4921 tggcatatat gggaaccttt cctcataagt tgagcgctta tcaatagaaa accattcagt
     4981 tacggtagga tgaacttctg atttttcatt agttccatgt gttgaacgaa tgtacgtagt
     5041 atttgttgac gggagattga cgactaaaat tcctgatttt ttgttaatgg tactatctct
     5101 catgctagag taaagttccc aatctacgtg ttttctgttt tttgtttcag ttccaacaag
     5161 aaggataaga actgatgtat ctctaagata ttcatctctt attatctctc ttattttttg
     5221 tggggcttca tcttcatcta tatcacctaa ggaaggtgcg aacaagtccc tgatatgaga
     5281 tcatgtttgt catctggagc catggaacag ggttcatcat gagtcatcaa ctcaccttcg
     5341 ccgacagtga attcagcagt aagcgccgtc agaccagaaa agagattttc ttgtcccgca
     5401 tggagcagat tctgccatgg caaaacatgg tggaagtcat cgagccgttt taccccaagg
     5461 ctggtaatgg ccggcgacct tatccgctgg aaaccatgct acgcattcac tgcatgcagc
     5521 attggtacaa cctgagcgat ggcgcgatgg aagatgctct gtacgaaatc gcctccatgc
     5581 gtctgtttgc ccggttatcc ctggatagcg ccttgcctga ccgcaccacc atcatgaatt
     5641 tccgccacct gctggagcag catcaactgg cccgccaatt gttcaagacc atcaatcgct
     5701 ggctggccga agcaggcgtc atgatgactc aaggcacctt ggtcgatgcc accatcattg
     5761 aggcacccag ctcgaccaag aacaaagagc agcaacgcga tccggagatg catcagacca
     5821 agaaaggcaa tcagtggcac tttggcatga aggcccacat tggtgtcgat gccaagagtg
     5881 gcctgaccca cagcctggtc accaccgcgg ccaacgagca tgacctcaat cagctgggta
     5941 atctgcttca tggagaggag caatttgtct cagccgatgc cggctaccaa ggggcgccac
     6001 agcgcgagga gctggccgag gtggatgtgg actggctgat cgccgagcgc cccggcaagg
     6061 taagaacctt gaaacagcat ccacgcaaga acaaaacggc catcaacatc gaatacatga
     6121 aagccagcat ccgg
//
