LOCUS       OW849007               12628 bp    DNA     circular BCT 17-MAY-2022
DEFINITION  Klebsiella pneumoniae isolate 273 genome assembly, plasmid: P4.
ACCESSION   OW849007
VERSION     OW849007.1
DBLINK      BioProject: PRJEB42440
            BioSample: SAMEA7853280
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
FEATURES             Location/Qualifiers
     source          1..12628
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="273"
                     /isolation_source="Hospital Universitario Gregorio Maranon
                     (Madrid)"
                     /db_xref="taxon:573"
                     /plasmid="P4"
     gene            1..848
                     /locus_tag="AI2713V1_REPA000000172"
     repeat_region   1..848
                     /locus_tag="AI2713V1_REPA000000172"
                     /note="ISKpn7_IS21_unknown"
                     /rpt_family="IS"
     gene            1..780
                     /locus_tag="AI2713V1_5264"
     CDS             1..780
                     /locus_tag="AI2713V1_5264"
                     /note="unnamed protein product; AI2713v1_5264.mrna"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAH3816443.1"
                     /translation="MNLQHERIDALCQQLKLERVATHYPVLAQQAVREDFSFTDFLEHL
                     LRHEAGCRQQRSRELLTRMACFPGIKTLEDYDFSFNPGVPKALVQELGSLAFVERAENV
                     VLIGPSGIGKTHLAIALGYKAAQAGVKTRFITAADLMLQLSTAQRQGRLKEYFNRAILG
                     PRLLVIDEIGYLPFGREEANLFFQVVAKRYEKGAMILTSNLPFSQWADAFAGDTTLTAA
                     MLDRLLHHAHILTLSGESYRLKDKRKAGVVRKNSKPE"
                     /name="AI2713V1_5264"
     exon            1..780
                     /locus_tag="AI2713V1_5264"
                     /note="AI2713v1_5264.mrna"
                     /number=1
     gene            1167..2048
                     /locus_tag="AI2713V1_5265"
     CDS             1167..2048
                     /locus_tag="AI2713V1_5265"
                     /note="unnamed protein product; AI2713v1_5265.mrna"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAH3816560.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="AI2713V1_5265"
     exon            1167..2048
                     /locus_tag="AI2713V1_5265"
                     /note="AI2713v1_5265.mrna"
                     /number=1
     gene            complement(2130..3669)
                     /locus_tag="AI2713V1_REPA000000171"
     repeat_region   complement(2130..3669)
                     /locus_tag="AI2713V1_REPA000000171"
                     /note="ISKpn6_IS1182_unknown"
                     /rpt_family="IS"
     gene            complement(2298..3617)
                     /locus_tag="AI2713V1_5266"
     CDS             complement(2298..3617)
                     /locus_tag="AI2713V1_5266"
                     /note="unnamed protein product; AI2713v1_5266.mrna"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAH3816678.1"
                     /translation="MAASYLPYRPDQSYLLPPSLGEWLPEGHLAYFISETVDTLDLSAF
                     HARYAGGGPGNQPFHPAMMVKVLIYGYASGVFSSRKLARKLYEDVALRVLAAGNFPAHR
                     TLSDFRALHLTELENLFVQVVQLARECGLVKLGTIAVDGTKVKANASRHKAMSYKRMKP
                     AEDELHCEIKALLDRAKATDDQERNEPELDIPAEISRREKRLEAIQAAKARLEARQREA
                     DQARGRSEDDGRRPRHPDGSDKGGGSYKREFGVPDDRDQESFTDPDSRIMKHAGGGSEQ
                     SYNGYTAVDAEHQIIVAAELTNCAADSQALLGMLAAVQANTGEMPAQTLADAGFRSEAV
                     LAKVADHHGDVIVALGREGREDAKVNAKTHPHTAAIAAKLKTEQGDAAYRRRKSIVEAP
                     NGWIKAVMGLRQFSMRGLDKVQAEWKLVCMALNLRRMAYL"
                     /name="AI2713V1_5266"
     exon            complement(2298..3617)
                     /locus_tag="AI2713V1_5266"
                     /note="AI2713v1_5266.mrna"
                     /number=1
     gene            complement(4551..5195)
                     /locus_tag="AI2713V1_5267"
     mRNA            complement(4551..5195)
                     /locus_tag="AI2713V1_5267"
                     /note="AI2713v1_5267.mrna"
     CDS             complement(4551..5195)
                     /locus_tag="AI2713V1_5267"
                     /note="long variant; AI2713v1_5267.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Pentapeptide repeat protein"
                     /protein_id="CAH3816794.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="Pentapeptide repeat protein"
     exon            complement(4551..5195)
                     /locus_tag="AI2713V1_5267"
                     /note="AI2713v1_5267.mrna"
                     /number=1
     gene            complement(6646..8361)
                     /locus_tag="AI2713V1_5268"
     mRNA            complement(6646..8361)
                     /locus_tag="AI2713V1_5268"
                     /note="AI2713v1_5268.mrna"
     CDS             complement(6646..8361)
                     /locus_tag="AI2713V1_5268"
                     /note="long variant; AI2713v1_5268.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Tyrosine recombinase XerD"
                     /protein_id="CAH3816902.1"
                     /translation="MKTGQTTASSSTPVGFPDADSLAALRGWYAGLSSRAAVEHYLAAR
                     KAAGQSARGMLGHIRRQLIVFANSRHRPDLADVFQHAPEERQGRAGAVERAIRLLPTLP
                     APKPQISDSIDQWLSPRTVRALQAYGIHTLSALTVRIPRRRGWWKAIEGLGMTGARQVE
                     AFFAAHPQLTERARALIPIIQPSGIVPWEQLRLPNEVDGSQGNFRAPRNACTLNATNDY
                     EAVQAWLALHETVATQRAYRKEAARLILWAIVERGRALSSLTTDDAIAYRAFVRHPTPR
                     ERWVGPPRPRDSVEWRPFSGGLSARSAAYALTVLSALFRWLIEQRYVLANPFAGVKVRG
                     HALRPALDTARGFTEGEWLLLRAIADGLEWSYGWSEPAAQRLRFMLDFGYATGLRASEL
                     VGAVLGNVRLDEHGDHWLHLVGKGGKPGKVALPPLAYTALDQYLAQRQLPVSRERWNPK
                     TPLVGSLGEDGNAGITGPHLWCVLRRFFTQAAEAIEADHPVAAEKLRRATPHWMRHSHA
                     SHALARGAQLTAVRDNLRHASIATTSMYLHGDELERARQMRQAFGARHVKRGVD"
                     /name="Tyrosine recombinase XerD"
     exon            complement(6646..8361)
                     /locus_tag="AI2713V1_5268"
                     /note="AI2713v1_5268.mrna"
                     /number=1
     gene            8471..11500
                     /locus_tag="AI2713V1_5269"
     mRNA            8471..11500
                     /locus_tag="AI2713V1_5269"
                     /note="AI2713v1_5269.mrna"
     CDS             8471..11500
                     /locus_tag="AI2713V1_5269"
                     /note="long variant; AI2713v1_5269.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3816920.1"
                     /translation="MSKKNKLLSVLSDAEQEALYGLPEFDDAQQLEYLAVTETELALAN
                     SRPSLHAKVYCLLQIGYFKAKHAFFSFDWDEVEDDCAFVLSRYFHGEVFEPKAITKHER
                     YTQREQIAKLFGYWPWSAAFLPQLEQQAARTVRRDVTPGFVAAELIVWLNEHKIIRPGY
                     TTLQELVSETLSAERRRLGNLLEQALDESAKAALSQLLVRDDTLSQLAALKQDAKNFGW
                     RQMVREREKRATLEPLHAIAKTLLPKLDVSQQNLLYYASLANFYTVHDLRNLKADQTQL
                     YLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFADEQVRRQQETPRVGRLLS
                     LYVDDSVADPTPFGEVRQRAYKIMPRDTLQTTAQRMSVKPASKLALQWQAVDGLADRMR
                     RHLRPLYVALDLSGSNPDSPWLAALAWAKGVFAKQQRLSQRPLDEWPAATLPKRLRPYL
                     LTFDADGKPTGLHADRYEFWLYRQIRKRLQSGELYLDDSLQHRHFSDELVEMDRKIDVL
                     AKMEIPFLQQPVHAQLDALTAELRTQWLAFNRELKQGKLTHLEYNKDTQKLTWRKPKSE
                     NEKAREKAFYEKLPFCDVADVFRFVNDECKFLSAFTPMQPRYAKKVADADSLMAVIIAQ
                     AMNHGNHVMARTSDIPYHVLDSGYQQYLRQATLHAANDCISNAIATLPIFPYYSFDLET
                     LYGAVDGQKFSVERPTVKARYSRKYFGRGKGVVAYTLLCNHIPLNGYLIGAHDYEAHHV
                     FDIWYRNTSDIMPTAITGDMHSVNKANFAILHWFGRRFEPRFTDLNAQLKELYCADDPA
                     QYQACLIRPVGKIDCDLIHREKPNIDRIVATLGLKEMTQGTLIRKLCTYTTTNPTRRAI
                     FEFDKLIRSIYTLRYLRDPQLERNVHRSQNRLESYHQLRSAIAQVGGKKELTGRTDIEI
                     EISNQCARLIANTIIFYNSAILSRLVTKYEAAGNSKALALITKISPAAWRHILLNGHYT
                     FQSSGKTIDLDAIVAGLELE"
                     /name="hypothetical protein"
     exon            8471..11500
                     /locus_tag="AI2713V1_5269"
                     /note="AI2713v1_5269.mrna"
                     /number=1
     gene            11521..12628
                     /locus_tag="AI2713V1_REPA000000173"
     repeat_region   11521..12628
                     /locus_tag="AI2713V1_REPA000000173"
                     /note="ISKpn7_IS21_unknown"
                     /rpt_family="IS"
     gene            complement(11889..>12590)
                     /locus_tag="AI2713V1_5270"
     mRNA            complement(11889..12590)
                     /locus_tag="AI2713V1_5270"
                     /note="AI2713v1_5270.mrna"
     CDS             complement(11889..>12590)
                     /locus_tag="AI2713V1_5270"
                     /note="short variant; AI2713v1_5270.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3817038.1"
                     /translation="VLERFQWYRAETVIQQGGLVAPHTGWQGLQVFPLLPPTVDGALVQ
                     GGVDPHIRLVCQPVTDASVGSSTIEQQPRALHPGQQRVVEALAEVAIEALDLAFGLGPI
                     RATQPDPEPQVIGQLKQAGVKTVLAVTVGIAFQHHGLHVVEQHLAGHTAQIVKSQQQGV
                     PHGLEGFVRYEGDKRHAAVSQRGDEGRHGGSGPAQDHEISLQLPSGLGLETDGRTCFAA
                     GGLERGEEGAQ"
                     /name="hypothetical protein"
     exon            complement(11889..12590)
                     /locus_tag="AI2713V1_5270"
                     /note="AI2713v1_5270.mrna"
                     /number=1
     gene            12275..12323
                     /locus_tag="AI2713V1_REPA000000170"
     repeat_region   12275..12323
                     /locus_tag="AI2713V1_REPA000000170"
                     /note="ISAeme17_IS21_unknown"
                     /rpt_family="IS"
     ARG             complement(4555..5195)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1168..2045
                     /name="blaKPC-2"
                     /gene="blaKPC-2"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          6174..6347
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(5704..6195)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 atgaacctcc agcatgagcg gatcgacgcc ctgtgccagc aactcaaact cgaacgggtg
       61 gcaacgcatt accccgtcct ggcgcagcag gcggtgcgcg aggacttcag cttcaccgac
      121 ttcctggaac acctgttgcg gcatgaagcg gggtgtcgcc agcagcgcag ccgcgaactg
      181 ctgacgcgga tggcttgctt cccgggcatc aagaccctgg aggactatga cttcagcttc
      241 aaccccggcg tgcccaaggc actggtgcag gagctgggca gtctggcgtt tgtcgaacgg
      301 gccgagaacg tggtgctgat cggaccctct ggcatcggca agacccacct cgccatcgcc
      361 ttgggttaca aggccgcgca agccggggtc aagacacgct tcatcacggc agccgacctg
      421 atgctgcaac tgagcacggc gcagcgacaa gggcggctga aggaatactt caaccgcgcc
      481 atcctcggtc cacggctgct ggtgatcgat gagatcggct acctgccttt cggacgcgag
      541 gaagcgaacc tgttctttca ggtggtggcg aagcgctatg agaaaggggc gatgatcctg
      601 acctcgaacc tgccgttcag ccagtgggcc gacgcgtttg ccggcgatac caccctgaca
      661 gccgcgatgc tggatcggct gctgcaccat gcccatatcc tgaccctgag cggcgaaagc
      721 taccgcttga aggacaagag gaaggcggga gtggtcagga aaaattccaa acccgaatga
      781 tccaggtggg tcagtattac tttggtgatt caggggtaaa gtgggtcagt tttcagttgg
      841 tgttgacacc ggcgtaccct cggtgctatc ttcgcgcccc aatagtcggg gcttggccag
      901 gacttcctga ggccgtccgt aacgtggatg ccgaggtcag gcgaggtggc cgacccatga
      961 acgccgacct gattcgtttt tcaatagcgc tggacgttgt ggtgccaggg acttaccaac
     1021 ccgatgtgtg cccatccggg gcagttacag ccgttacagc ctctggagag ggagcggctt
     1081 gccgctcggt gataatccca gctgtagcgg cctgattaca tccggccgct acacctagct
     1141 ccaccttcaa acaaggaata tcgttgatgt cactgtatcg ccgtctagtt ctgctgtctt
     1201 gtctctcatg gccgctggct ggcttttctg ccaccgcgct gaccaacctc gtcgcggaac
     1261 cattcgctaa actcgaacag gactttggcg gctccatcgg tgtgtacgcg atggataccg
     1321 gctcaggcgc aactgtaagt taccgcgctg aggagcgctt cccactgtgc agctcattca
     1381 agggctttct tgctgccgct gtgctggctc gcagccagca gcaggccggc ttgctggaca
     1441 cacccatccg ttacggcaaa aatgcgctgg ttccgtggtc acccatctcg gaaaaatatc
     1501 tgacaacagg catgacggtg gcggagctgt ccgcggccgc cgtgcaatac agtgataacg
     1561 ccgccgccaa tttgttgctg aaggagttgg gcggcccggc cgggctgacg gccttcatgc
     1621 gctctatcgg cgataccacg ttccgtctgg accgctggga gctggagctg aactccgcca
     1681 tcccaggcga tgcgcgcgat acctcatcgc cgcgcgccgt gacggaaagc ttacaaaaac
     1741 tgacactggg ctctgcactg gctgcgccgc agcggcagca gtttgttgat tggctaaagg
     1801 gaaacacgac cggcaaccac cgcatccgcg cggcggtgcc ggcagactgg gcagtcggag
     1861 acaaaaccgg aacctgcgga gtgtatggca cggcaaatga ctatgccgtc gtctggccca
     1921 ctgggcgcgc acctattgtg ttggccgtct acacccgggc gcctaacaag gatgacaagc
     1981 acagcgaggc cgtcatcgcc gctgcggcta gactcgcgct cgagggattg ggcgtcaacg
     2041 ggcagtaagg ctctgaaaat catctattgg cccaccaccg ccgcccttgc gggcggcatg
     2101 gattaccaac cactgtcaca tttaggctag gagtctgcgc ggcagagccg tgtgaccggt
     2161 tttctgtaga gcactgacga tggcggcggc gctctctgca attggcaagg cgtcggcgcc
     2221 aaggatacca atcttgcggc gcgcggcgtg ttatgacgac tggggtgcat ttgagccgcc
     2281 ccatttaacc ttcgccctca cagatacgcc attcgcctca gatttagcgc catgcagacg
     2341 agcttccact cggcttgcac cttgtccagg cccctcatgc tgaactgacg caatcccatc
     2401 accgccttga tccaaccatt cggagcctcc acgatcgact tgcgccggcg gtaagctgca
     2461 tcgccttgct ccgttttcaa tttcgccgca atcgccgccg tatgcggatg ggtcttggca
     2521 ttgaccttgg catcttcacg tccctcgcgg ccgagggcaa cgatgacatc gccgtggtga
     2581 tcggcgacct ttgccagaac agcctcacta cggaatcccg catccgccag cgtctgggcc
     2641 ggcatttctc cggtgttggc ctgaactgct gccagcatgc ccagcagcgc ctgactgtcc
     2701 gcggcgcagt tggtcaactc cgccgccacg atgatctggt gctcggcatc gaccgctgtg
     2761 tacccgttgt agctctgctc ggagccacca ccggcgtgtt tcatgatccg gctgtccgga
     2821 tcggtgaagc tttcctgatc acggtcatcc ggcacaccaa actcgcgttt gtacgagcca
     2881 ccgcccttgt ccgagccatc cggatggcga ggccggcggc catcgtcttc gctgcgcccc
     2941 cgggcctggt ccgcttcacg ctggcgcgct tccaggcgcg cctttgccgc ctggatcgcc
     3001 tccaggcgct tctcgcggcg agaaatctcg gcaggaatgt ccagctccgg ctcgttacgc
     3061 tcctggtcgt cggtagcctt ggcgcgatca agcagcgcct tgatctcgca atgcaattcg
     3121 tcctcggccg gcttcatgcg cttatagctc atcgccttgt ggcggctggc gttggctttt
     3181 accttggtgc cgtcgaccgc gatcgtgcca agcttcacca gcccacattc gcgcgccagt
     3241 tgcaccacct gaacgaacaa attctcaagc tcggtcaggt gtagggcacg gaagtcactc
     3301 agcgtgcggt gggccgggaa gtttccagcg gccagcacac gcaacgcgac atcctcgtac
     3361 agcttcctgg ctagtttgcg cgaagagaag acgccgctcg cgtaaccata gatcagcact
     3421 ttgaccatca tcgccggatg aaacggctga ttgcccggac caccgccggc ataccgggcg
     3481 tggaatgcgc tcaagtccag cgtatcgaca gtctcgctga tgaagtaggc aagatgccct
     3541 tcaggtagcc actctcccag agaagggggc agcagatagg attggtcggg tcggtaagga
     3601 aggtaactgg cagccatccc cgtatcgtcc tcgatcttcc gccgatttgg cttctgccgc
     3661 gcaggctcct aggccaccgt agctaaacat acgagaaaaa ccgcttcttg agcgctctct
     3721 gagaccacct tgccgacaac gcagaaacgg ttgcggaaaa atcggcgttt ccggcgtacg
     3781 ccacccgacc tggacgaact attgcggacc tggaactgga gtagcggaat tccccggggt
     3841 ttcagcgtcg cgccacagcg cctcagatag atgcggtagc ctttggcggg tcgtcatgcc
     3901 gcggaccacc cccccaaaaa attccacaat gcggaatggc ggaattttct ggggttcccg
     3961 cttacccccc caggtaaaaa gctaaatcag cctaaccggc acatcgtcct gaacagagcg
     4021 cgtgcgcaaa ttgagttgca gcgttacgcc gaccgccagc gtgcattacg tgaggatgag
     4081 cggcagcagt ccgattttgc ctggtcacgt ccgcgcgcgc cgcgccgctg attttggctt
     4141 ttggcggttt agtggtgctg acgctgcgaa atcacctgcc attcacctgc gtgcatagcc
     4201 atgcagtcgc gcagaaatca tcaggggcgt ttttccggga tactggtgag gaaaggtgct
     4261 ggaaacggcc ctgatgcagc aggattgcgc tgagggcgcc tgcgggggtg cgggcaaggg
     4321 atgttatgtt taatcggtct gaccgtcagg tacaaaaaaa agcccttagg gggctttttt
     4381 tttgctatga gttagccata ttttcgtgcg aatttttcat cggaatcgca tagataaata
     4441 ataaactcaa taaacgcgat gatcgcgggt atgaatgtcc agcaaaaaat tagatacagg
     4501 aaccccttaa aagtaaatcg tcaaacgcat ctcccggtgt aaacgctgaa ctaaccaatc
     4561 acagcgatgc caagccgctc catgagcaac gatgcctggt agctgtctaa cttaacgcct
     4621 tgtaaatcaa caccccgaat atctaagtca cctaactccg aattggtcag atcgcaatgt
     4681 gtgaagtttg ctgctcgcca gtcgaaagtc gaaaactcgc cgccggagag atctgaacca
     4741 ctgaacgtcg cacccagtac ctgagtcccc atccagcggt tttcccacag ctcacacttt
     4801 tccaacacga ctttcgaaaa attggcgtag cttagattag tattagtgat atatgcgctg
     4861 caaaaccagg tgcgcgtggt gatcatattc ataaagcttg caccgcggaa atctgcgcct
     4921 tgtgcgcggc agtggcgaat ttcaatgccc aaggcactga cgttgcggaa atctgccatt
     4981 gataaatcac agcttttgaa aatggcatct ttcagcattg cgcgactaaa attgcaccct
     5041 ttctgacttt cgcgatcata gaactggcag ccgataaatt cagtgccgct caggtcggca
     5101 cctgaaaaat cgcagttaaa aaatgtacta ttttcaactt tctcaccggt gaagcgattt
     5161 ctgtcaattt tttcgccaac taatgccaga gtcatatcta tgcctgtgtt tttatacagt
     5221 aatgacgcca tggtaaacct tatgacgtta tgcgtcaaac ccgccagtat gacacctgca
     5281 aatgcgtatt agatctggtg tttcttcagc aaggcacgta gctgatgata agtcagaccc
     5341 agtaattcag cggctttttt ctggttaaat ttagcctgct gtaagctggt ttgtagaaag
     5401 tctttctctt gctgctgctg gaattcacgc agatccagcg ttataacgat gtgtatccta
     5461 tgtgctcgga ttggatagat ttccattacg gtgaaggatc ttcttgagat cctttctgtg
     5521 gcgtcgtaat ctcttgctct gtaaacgaaa aaaccgccct gcaaggcggt ttttcgaagg
     5581 ttctcagagc agcaactctt tgaaccgtgg taactggctt acgaggagcg cagtaaccaa
     5641 atctgttctt tcagtttagc cttaacaggc gcataacttc aagactaact cctctaaatc
     5701 agttaccagt ggctgctgcc agtggcgata agtcgtgtct ttccgggttg gactcaagat
     5761 gatagttacc ggataaggcg cagcagtcgg actgaacggg gggttcgtgc atacagtcca
     5821 tccaggagcg aactgccttc gcggaactga gtgtcaggcg tggaatgaga taaacgcggc
     5881 cataacagcg gaatgacacc ggtaaaccga aaggcaggaa tgcgggggag cacgagggag
     5941 ccaccagggg gaaacgcctg gtatctttaa gccgcatcgg gtttcgccac cactgatttg
     6001 agcgtcagat ttcatgatgt ttgtcagggg ggcggagcct atggaaaaac ggcttcgccg
     6061 cggccttatc tcttccctgt taagctcctt cctggtatct cccaggaaat ttccgccccg
     6121 cctgtaagcc ggcaccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6181 ggaagcggaa tatatcctgt actacatatc ctgctgacgc gctggtgccg ccttttttct
     6241 cctgctacat gaagcacttc actgatttcc acatccgtgc caacatagtc agccagtata
     6301 cactccgcta gcgctacgtg actggttcag ggctgcgccc cgaaaccggc taaaagctaa
     6361 tgacgcgcct gcggcttgtc caacgcctca cggcaccgga ttaaaatccg ctcccgttca
     6421 ccgtttctga ctgcctttca ccgtcgctgg cgcttcatcg tttctactgt gtctggcaga
     6481 ctgttctgat ttattctgac tggagaacgt tatggccaga ctgacaaact tgacgcctgc
     6541 agagaaaaaa tttcttgatg atgctgttgc cgcagctgaa agagcgtcag gtaggggttc
     6601 taagcgggaa tcccagaaaa ttccgtcatt ccgtatgatg aaaattcagt cgactcctcg
     6661 cttgacgtga cgagcgccaa aagcctgccg catctgcctg gcgcgctcga gctcatcgcc
     6721 atgcaaatac atcgacgtcg tggcgatcga cgcatggcgt aggttgtcgc gcaccgcggt
     6781 gagctgagct ccccgggcca acgcatggct ggcatgactg tgacgcatcc aatgaggcgt
     6841 ggcacggcgc agcttttcgg cggcgaccgg atgatcggcc tcaatggcct cagcggcttg
     6901 cgtaaagaat cttcgcaaca cgcaccaaag gtgagggccg gtgatgccgg cattaccgtc
     6961 ttccccaaga ctgcccacca ggggcgtttt cggattccac cgttcccggc tgactggcag
     7021 ttgacgctgt gccaagtatt ggtccagcgc cgtatacgcg agcggcggca aggccacctt
     7081 acctggtttg ccacctttgc caaccaaatg cagccagtgg tcaccgtgct cgtcgaggcg
     7141 gacattaccc aggacggcgc cgaccagttc gctagcgcgc aggcccgtgg catagccgaa
     7201 gtcgagcatg aagcgcaggc gttgtgctgc cggctcggac cagccgtagg accattccag
     7261 gccgtctgca atggcgcgca gcagtagcca ttctccctcg gtaaagcctc gcgccgtatc
     7321 cagtgcgggc cgcaaggcgt gaccgcgtac cttgacgccg gcgaaagggt tggccagcac
     7381 atagcgctgc tcgatgagcc accggaacag cgccgagagc accgtcagcg catacgctgc
     7441 cgagcgtgcg gacaagccgc ccgagaaggg gcgccattcc accgaatcgc gtggtcgagg
     7501 tggccccacc caacgctcgc gcggggtcgg gtgccggaca aaggcccggt acgcgattgc
     7561 atcgtccgtg gtcagggatg atagcgcccg tccgcgttcg acgatggccc acaggattag
     7621 ccgcgcagcc tccttgcgat aggcgcgctg tgtggcgaca gtctcgtgca gcgccagcca
     7681 ggcctgcacg gcctcgtagt cgttggtcgc attcagcgtg caagcgttgc gcggggcccg
     7741 gaaattgccc tgcgagccgt cgacttcgtt cggcagacgc aactgctccc acggcacgat
     7801 gccgctgggc tgaataatcg ggatcagggc ccgggcgcgc tcggtgagct gcggatgtgc
     7861 ggcaaagaaa gcctcgacct gccgggcgcc tgtcatgccg agtccctcga tggctttcca
     7921 ccagccacgc cggcggggaa tgcgcacggt cagcgccgag agggtgtgga taccgtacgc
     7981 ctgcagtgcc ctgacggtcc tcggagacag ccactggtcg atgctgtcgc tgatctgggg
     8041 cttcggcgcc ggcagcgtgg gcaagagccg gatcgcgcgt tcgacggcac ccgcgcgccc
     8101 ctgtcgctct tcgggggcgt gctggaacac gtcggccaga tccggccggt ggcggctgtt
     8161 ggcgaaaacg atgagctggc ggcggatatg ccccagcatc cctcgcgctg attgaccggc
     8221 ggccttgcgt gccgccaggt agtgttcaac ggccgcgcga gagctgagtc ccgcgtacca
     8281 gccgcgcaag gcagccagcg aatccgcatc gggaaagccg actggggtag aactcgaagc
     8341 cgtagtctgg cccgttttca tatggacagg ttgcgattcg aaaagcgagt aacgcgataa
     8401 taacgattat ttcagtagaa tcgaaaatgc cccatgtttc tacgaaacct caagatagca
     8461 agaacgggcc atgagcaaga agaacaagct actcagcgtc ctatccgacg ccgaacagga
     8521 ggccttgtat ggcctgccgg agttcgatga tgcgcagcag ctggaatatc tggctgtgac
     8581 agaaaccgag ttggcgcttg ccaacagccg gcccagtctt catgccaagg tctattgcct
     8641 cttgcagatc ggctacttca aggccaagca tgccttcttc agtttcgact gggacgaagt
     8701 cgaggacgat tgcgctttcg tgctgagccg ctatttccac ggcgaggtgt tcgaacccaa
     8761 ggcgatcacc aagcatgaac gctacaccca gcgcgagcag atcgccaaac tattcggcta
     8821 ctggccgtgg tcggccgcct tcctgccgca gctcgagcag caggccgcac ggaccgtgcg
     8881 gcgtgacgta acgccggggt tcgtcgccgc cgagctgatc gtctggctca atgagcacaa
     8941 gattatccgg cccggctata cgaccttgca agagttggtc agcgaaacgc tatccgccga
     9001 gcgccggcgc ctgggcaatc tgctggagca agcgttggac gaatctgcca aggccgcgct
     9061 gagccagctt ctggtccgtg atgacaccct gtcgcaactg gcggcgctca agcaggacgc
     9121 caagaatttc ggctggcgcc agatggtccg cgagcgcgaa aagcgcgcca cgctggagcc
     9181 gctgcacgca atcgccaaga cactgctgcc taaactcgac gtctcgcagc agaacctgct
     9241 gtactacgcg agcctggcaa acttctacac cgtccacgac ctgcgcaatc tgaaggccga
     9301 tcagacccaa ctctacctgc tgtgctatgc ctgggtacgc taccggcagc tctccgacaa
     9361 cctggtcgat gcgatggcgt accacatgaa gcagttggag gacgaaagca gcgctggtgc
     9421 caaacaatcc tttgccgacg agcaggtgcg ccgtcagcag gaaacgccgc gggtaggccg
     9481 cctgctgtca ctctacgttg atgatagcgt ggccgatccc acgcccttcg gcgaggtgcg
     9541 gcagcgcgcc tacaagatca tgcctcgcga tacgctgcaa accacggcgc agcgtatgag
     9601 cgtgaagccg gcgagcaagc tggccttgca gtggcaggcg gtggacgggc tggccgaccg
     9661 catgcgccgc catcttcgtc cactgtacgt tgcgctcgac ctctccggct ccaatccaga
     9721 cagtccatgg cttgccgcgc tggcctgggc caaaggtgtt tttgccaagc agcagcggct
     9781 gtcgcaacgg ccactcgacg agtggcccgc ggcgacactt ccgaaacgct tgcggccgta
     9841 cctgctgacc ttcgacgccg acggtaaacc aaccggcctg cacgctgacc gctacgagtt
     9901 ctggctgtac cgccagatca ggaagcgtct ccaatcgggt gaactctacc tcgacgacag
     9961 cctgcaacac cggcacttct cagatgaact ggttgagatg gacaggaaga tcgatgtgct
    10021 cgcaaaaatg gagatcccct tcctgcagca acccgtccat gcccaactcg atgcgctgac
    10081 tgccgaactg cgcacgcagt ggctggcgtt caaccgcgag ctgaaacagg gcaagctgac
    10141 gcacctcgaa tacaacaagg acacgcagaa actgacctgg cgcaagccca agagcgagaa
    10201 tgagaaagcg cgcgaaaagg cattctacga gaagcttccg ttctgcgacg tggccgacgt
    10261 gtttcgcttc gtcaacgacg agtgcaagtt tctgtcggcg ttcacgccca tgcagccgcg
    10321 ctacgcgaag aaggtcgccg acgctgacag cctgatggcg gtcatcatcg cgcaagcgat
    10381 gaaccacggc aaccacgtca tggcgcgcac cagcgacatc ccgtaccacg tgctggatag
    10441 cggctaccag cagtacctgc gccaggcaac gctgcacgcg gccaacgact gcatcagcaa
    10501 cgccatcgcc acgctaccga tcttcccata ctactcgttc gacctcgaaa cgctgtacgg
    10561 tgccgtcgac gggcagaaat tcagcgtcga acggccgacc gtgaaggcgc gctattcgcg
    10621 caagtacttc gggcgcggca agggcgtggt cgcctacacg ctgctgtgca accacatccc
    10681 gctcaacggc tacctgatcg gcgcgcacga ctacgaggcc caccacgtgt tcgacatctg
    10741 gtatcgcaac acgtcggaca tcatgccgac cgcgatcacc ggcgacatgc acagcgtcaa
    10801 caaggccaac ttcgccatcc tgcattggtt tggccggcgg ttcgagccgc gcttcaccga
    10861 cctcaacgcg caattgaaag aactctactg cgctgacgat ccagcacagt atcaggcgtg
    10921 cctgattcgg ccagtcggga aaatcgactg cgatctcatc catcgcgaga agccgaacat
    10981 cgaccggatc gtcgccacac tcgggctgaa ggaaatgacg cagggcacgc tgatccgcaa
    11041 gctgtgcacc tacaccacga cgaacccgac gcggcgcgcg atcttcgagt tcgacaagct
    11101 catccgcagc atctacacgc ttcgctacct gcgcgatccg caactggaac gcaacgtgca
    11161 ccgctcgcaa aatcgcctcg agtcctacca tcagctacgc tcggccattg cccaggtcgg
    11221 cggtaagaag gaactgaccg gtcgcaccga cattgaaatc gagatcagca accagtgcgc
    11281 acggctgatc gccaacacga tcattttcta caactcggca atcttgtcgc ggctggtgac
    11341 caagtacgag gcggccggca acagcaaggc gctggcactc atcacaaaaa tttcgcctgc
    11401 ggcatggcgg cacatcctgc tcaacgggca ctacaccttt caaagcagcg gcaagacgat
    11461 cgatctcgat gcgatcgtcg caggcctgga actggagtga cggaattttc agcggttccg
    11521 tgttagcagc agtgtaaaac tgacccaccc cagcgaagta aaactgatcc acctgggcta
    11581 gtttggcggc cttttcgagg ccgtcgatgt tgacccagga gcaaaccgtg gaaatcagag
    11641 ttcttgcccg acagggccac tccatccggc acatcgcccg cacgttgggg gtctcgcgca
    11701 ataccgtgcg ccgctacctc cgcgatccgt cggtagcgcg ataccagccc cgtgaaccac
    11761 gccccaccaa actggggcca tttgaatctt atctgcggca acgggtcgag caggcccacc
    11821 ccatctggct gcctgccacc gtcctgaatc gggaaatccg cgctcaaggg tatggcggcg
    11881 gtctctcgtt attgcgcgcc ttcctcgcca cgctcaagcc cgcccgccgc gaagcaggtc
    11941 cggccgtccg tttcgagacc gagcccggac ggcagttgca ggctgatttc gtggtcttgc
    12001 gccgggccag atcccccatg tcggccttcg tcgccacgct gggatacagc cgcatgacgt
    12061 ttgtcacctt cgtaccggac gaatccttcg agtccgtgcg ggactccctg ttgctggctt
    12121 ttgactatct gggcggtgtg ccccgcgagg tgttgttcga caacatgaag accgtggtgc
    12181 tggaacgcga tgcctacggt gacggcaagc accgttttca ccccggcttg cttcaactgg
    12241 ccgatgacct ggggttccgg atccggctgt gtcgcccgta tcgggcccag accaaaggca
    12301 aggtcgagcg cttcaatcgc tacttccgcg agagcttcta caacccgttg ctgacccgga
    12361 tgaagggcac ggggctgctg ctcgattgtg ctgctgccaa ccgacgcgtc cgtgactggt
    12421 tggcagacga ggcgaatgtg cgggtccacg ccaccctgaa cgagcgcccc atcgaccgtt
    12481 ggcggcagga gcgggaacac ctgcaaccct tgccatcccg tgtgcggcgc gacgaggccc
    12541 ccctgctgga taacagtctc cgcccggtac cactggaatc tctccagcac ccactgtctg
    12601 tctatgacgc aattggggag gcctgccg
//
