LOCUS       OW848988               14708 bp    DNA     circular BCT 17-MAY-2022
DEFINITION  Klebsiella pneumoniae isolate 258 genome assembly, plasmid: P2.
ACCESSION   OW848988
VERSION     OW848988.1
DBLINK      BioProject: PRJEB42440
            BioSample: SAMEA7853288
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
FEATURES             Location/Qualifiers
     source          1..14708
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="258"
                     /isolation_source="Hospital Universitario Virgen de las
                     Nieves (Granada)"
                     /db_xref="taxon:573"
                     /plasmid="P2"
     gene            1015..1143
                     /locus_tag="AI2723V1_5323"
     mRNA            1015..1143
                     /locus_tag="AI2723V1_5323"
                     /note="AI2723v1_5323.mrna"
     CDS             1015..1143
                     /locus_tag="AI2723V1_5323"
                     /note="long variant; AI2723v1_5323.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3821570.1"
                     /translation="MEKRHLRGLAVLPHMLVPCLFTESVGNFACVRSARRSLTAGA"
                     /name="hypothetical protein"
     exon            1015..1143
                     /locus_tag="AI2723V1_5323"
                     /note="AI2723v1_5323.mrna"
                     /number=1
     gene            1855..3819
                     /locus_tag="AI2723V1_5324"
     mRNA            1855..3819
                     /locus_tag="AI2723V1_5324"
                     /note="AI2723v1_5324.mrna"
     CDS             1855..3819
                     /locus_tag="AI2723V1_5324"
                     /note="long variant; AI2723v1_5324.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3821679.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="hypothetical protein"
     exon            1855..3819
                     /locus_tag="AI2723V1_5324"
                     /note="AI2723v1_5324.mrna"
                     /number=1
     gene            3819..4550
                     /locus_tag="AI2723V1_5325"
     mRNA            3819..4550
                     /locus_tag="AI2723V1_5325"
                     /note="AI2723v1_5325.mrna"
     CDS             3819..4550
                     /locus_tag="AI2723V1_5325"
                     /note="long variant; AI2723v1_5325.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3821784.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="hypothetical protein"
     exon            3819..4550
                     /locus_tag="AI2723V1_5325"
                     /note="AI2723v1_5325.mrna"
                     /number=1
     gene            4557..5087
                     /locus_tag="AI2723V1_5326"
     mRNA            4557..5087
                     /locus_tag="AI2723V1_5326"
                     /note="AI2723v1_5326.mrna"
     CDS             4557..5087
                     /locus_tag="AI2723V1_5326"
                     /note="long variant; AI2723v1_5326.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3821896.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     exon            4557..5087
                     /locus_tag="AI2723V1_5326"
                     /note="AI2723v1_5326.mrna"
                     /number=1
     gene            complement(5115..5294)
                     /locus_tag="AI2723V1_5327"
     mRNA            complement(5115..5294)
                     /locus_tag="AI2723V1_5327"
                     /note="AI2723v1_5327.mrna"
     CDS             complement(5115..5294)
                     /locus_tag="AI2723V1_5327"
                     /note="long variant; AI2723v1_5327.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Regulatory protein rop"
                     /protein_id="CAH3822004.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Regulatory protein rop"
     exon            complement(5115..5294)
                     /locus_tag="AI2723V1_5327"
                     /note="AI2723v1_5327.mrna"
                     /number=1
     gene            5363..5740
                     /locus_tag="AI2723V1_5328"
     mRNA            5363..5740
                     /locus_tag="AI2723V1_5328"
                     /note="AI2723v1_5328.mrna"
     CDS             5363..5740
                     /locus_tag="AI2723V1_5328"
                     /note="long variant; AI2723v1_5328.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3822114.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     exon            5363..5740
                     /locus_tag="AI2723V1_5328"
                     /note="AI2723v1_5328.mrna"
                     /number=1
     gene            complement(5802..6101)
                     /locus_tag="AI2723V1_5329"
     mRNA            complement(5802..6101)
                     /locus_tag="AI2723V1_5329"
                     /note="AI2723v1_5329.mrna"
     CDS             complement(5802..6101)
                     /locus_tag="AI2723V1_5329"
                     /note="long variant; AI2723v1_5329.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3822227.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="hypothetical protein"
     exon            complement(5802..6101)
                     /locus_tag="AI2723V1_5329"
                     /note="AI2723v1_5329.mrna"
                     /number=1
     gene            complement(6091..6354)
                     /locus_tag="AI2723V1_5330"
     mRNA            complement(6091..6354)
                     /locus_tag="AI2723V1_5330"
                     /note="AI2723v1_5330.mrna"
     CDS             complement(6091..6354)
                     /locus_tag="AI2723V1_5330"
                     /note="long variant; AI2723v1_5330.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3822341.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     exon            complement(6091..6354)
                     /locus_tag="AI2723V1_5330"
                     /note="AI2723v1_5330.mrna"
                     /number=1
     gene            complement(6660..>6992)
                     /locus_tag="AI2723V1_5331"
     mRNA            complement(6660..6992)
                     /locus_tag="AI2723V1_5331"
                     /note="AI2723v1_5331.mrna"
     CDS             complement(6660..>6992)
                     /locus_tag="AI2723V1_5331"
                     /note="short variant; AI2723v1_5331.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3822453.1"
                     /translation="LLNVSFRSTERQTPFDPNFSTQKADTTRMMVPFFRQFWDMGAKDK
                     ATGKSRSDVQQRIQQFHSQEFLNSLQGTTQFAGTDYRSKDLTPKKSRLLADTISAVYLD
                     GYEGRQ"
                     /name="hypothetical protein"
     exon            complement(6660..6992)
                     /locus_tag="AI2723V1_5331"
                     /note="AI2723v1_5331.mrna"
                     /number=1
     gene            complement(6985..9990)
                     /locus_tag="AI2723V1_5332"
     mRNA            complement(6985..9990)
                     /locus_tag="AI2723V1_5332"
                     /note="AI2723v1_5332.mrna"
     CDS             complement(6985..9990)
                     /locus_tag="AI2723V1_5332"
                     /note="long variant; AI2723v1_5332.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3822563.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="hypothetical protein"
     exon            complement(6985..9990)
                     /locus_tag="AI2723V1_5332"
                     /note="AI2723v1_5332.mrna"
                     /number=1
     gene            10152..10709
                     /locus_tag="AI2723V1_5333"
     mRNA            10152..10709
                     /locus_tag="AI2723V1_5333"
                     /note="AI2723v1_5333.mrna"
     CDS             10152..10709
                     /locus_tag="AI2723V1_5333"
                     /note="long variant; AI2723v1_5333.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Transposon Tn3 resolvase"
                     /protein_id="CAH3822684.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="Transposon Tn3 resolvase"
     exon            10152..10709
                     /locus_tag="AI2723V1_5333"
                     /note="AI2723v1_5333.mrna"
                     /number=1
     gene            10892..11497
                     /locus_tag="AI2723V1_5334"
     mRNA            10892..11497
                     /locus_tag="AI2723V1_5334"
                     /note="AI2723v1_5334.mrna"
     CDS             10892..11497
                     /locus_tag="AI2723V1_5334"
                     /note="long variant; AI2723v1_5334.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside N(6')-acetyltransferase type 1"
                     /protein_id="CAH3822792.1"
                     /translation="MSIQHFQTKLGITKYSIVTNSNDSVTLRLMTEHDLAMLYEWLNRS
                     HIVEWWGGEEARPTLADVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGW
                     WEEETDPGVRGIDQLLANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAI
                     RCYEKAGFERQGTVTTPDGPAVYMVQTRQAFERTRSVA"
                     /name="Aminoglycoside N(6')-acetyltransferase type 1"
     exon            10892..11497
                     /locus_tag="AI2723V1_5334"
                     /note="AI2723v1_5334.mrna"
                     /number=1
     gene            <11579..11836
                     /locus_tag="AI2723V1_5335"
     mRNA            11579..11836
                     /locus_tag="AI2723V1_5335"
                     /note="AI2723v1_5335.mrna"
     CDS             <11579..11836
                     /locus_tag="AI2723V1_5335"
                     /note="short variant; AI2723v1_5335.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptomycin 3''-adenylyltransferase"
                     /protein_id="CAH3822904.1"
                     /translation="VIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDID
                     LLVTVTVRLDETTRRALINDLLETALLQIVGGDKLIIPFC"
                     /name="Streptomycin 3''-adenylyltransferase"
     exon            11579..11836
                     /locus_tag="AI2723V1_5335"
                     /note="AI2723v1_5335.mrna"
                     /number=1
     gene            11848..12552
                     /locus_tag="AI2723V1_5336"
     mRNA            11848..12552
                     /locus_tag="AI2723V1_5336"
                     /note="AI2723v1_5336.mrna"
     CDS             11848..12552
                     /locus_tag="AI2723V1_5336"
                     /note="long variant; AI2723v1_5336.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3823005.1"
                     /translation="MNPFKGRHFQRDIIRWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="hypothetical protein"
     exon            11848..12552
                     /locus_tag="AI2723V1_5336"
                     /note="AI2723v1_5336.mrna"
                     /number=1
     gene            13184..14581
                     /locus_tag="AI2723V1_5337"
     mRNA            13184..14581
                     /locus_tag="AI2723V1_5337"
                     /note="AI2723v1_5337.mrna"
     CDS             13184..14581
                     /locus_tag="AI2723V1_5337"
                     /note="long variant; AI2723v1_5337.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Colicin-E3"
                     /protein_id="CAH3823126.1"
                     /translation="MAFGLPALATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLW
                     GIAIYGVLPSEIAKDDPKMMSKIVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKD
                     ERQHIAVVTGRPMSVPVVDAKPTKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIA
                     EKGDSRPAGFTAGGNSREAVIRFPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAW
                     DAAHPEEGLKREYDKAKAELDAEDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAE
                     ANKDDFVTYNPPHEYGSGWQDQVRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALS
                     GAMESRKQKEKKAKDAENKLNEEKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPK
                     TPKQGGGGKRARWYGDKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPK
                     RNIKKYL"
                     /name="Colicin-E3"
     exon            13184..14581
                     /locus_tag="AI2723V1_5337"
                     /note="AI2723v1_5337.mrna"
                     /number=1
     ARG             10944..11494
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             11580..11836
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             10893..11497
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     BGC             7893..14708
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(478..1056)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3820..4547
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 taaaggataa tattaataat ggttggtttg atgttgaaaa atcatgggtt tcgatattac
       61 agccacactt taaaaatgta atcgatatta gtaaatttga ttactttgta tcctttgttt
      121 accgggatgg taactggtaa gcaaaagtta tcaaggatga tttgaaatac agacttccgg
      181 tgttcaggga tgaataccgg ggtttttaaa tacagggaag gtatatgaaa aaggtaaagg
      241 ttgtttttct ttttatattg attttttcag gctttcttct ggtcgcatgt caggcaaact
      301 atatccggga tgttcagggt ggaacggtgg caccatcgtc ctcctctgaa ctgacgggga
      361 tcgcggttca gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
      421 cttttgccct gtaaacgaaa aaaccacctg gggaggtggt ttgatcgaag gttaagtcag
      481 ttggggaact gcttaaccgt ggtaactggc tttcgcagag cacagcaacc aaatctgtcc
      541 ttccagtgta gccggacttt ggcgcacact tcaagagcaa ccgcgtgttt agctaaacaa
      601 atcctctgcg aactcccagt taccaatggc tgctgccagt ggcgttttgc gtgcttttcc
      661 gggttggact caagtgaaca gttaccggat aaggcgcagc agtcgggctg aacggggggt
      721 tcttgcttac agcccagctt ggagcgaacg acctacaccg agccgagata ccagagtgtg
      781 agctatgaga aagcgccaca cttcccgcaa gggagaaagg cggaacaggt atccgggaaa
      841 cggcagggtt ggaacaggag agcgcaagag ggagcgattc atcggaaacg gtgatgatct
      901 ttaagtcctg tcgggtttcg ccactcctga ctgattcatg gttgagccac cggctcccac
      961 agatgcaccg aaaaagcgtc tgtttatgtg aactcagtca ggagggcgga gcctatggaa
     1021 aaacgccacc tgcgtggcct tgccgttttg cctcacatgt tagtcccctg cttattcacg
     1081 gaatctgtgg gtaactttgc atgtgtccgc agcgcccgcc gcagtctcac ggccggagcg
     1141 tagcgaccga gtgagcgagg aggcgcaatt tccccggcac ctacaagacg gacaccggac
     1201 gcgctgatgt ggcatattgt cccctgatta agtctttata ttccctgata ataaattacc
     1261 aggataggtg aatcatatcg gtttttcgct gaacatttta cgttaaccta tctctgttgc
     1321 ttttttctcc ttacggagaa atacaggtca tccccgcgag ggcgtggctg acctcagggg
     1381 gatatcccct gcttacgctg gctggacgcc accgacgcac ggtctctccc cctctttctg
     1441 gctccgtccg gctggacgcc ggccacgcca gaaattcacc cggcccccgt caggctcacg
     1501 cctgaccacc cacaaaaatc cacggtgctg gacgcacctg gcgaagcagc aaagctgctc
     1561 ctgattttgc gggtggtcag ccgctcgcta tggcgaactc tgacgggggc cggaacaccg
     1621 gaaaagaccg cactggggtg ccgccttttc ctctcacacg ctggggcgtg ctcgttgttc
     1681 ctccggggct gcgccttttc cgcatggggc ggaaagaggc tggcctccgt ggtgaaagct
     1741 gaacgcaatc accacggacg tgaccgccgc cgccatgcct tggggcatgt ccgcggcttg
     1801 tgcagcgggg gctgcacatc acgccggcaa accatcgtct gaaggagaga gaacatgttt
     1861 aatacggatt cgcttgcctg gaagtggcac attccggagc tggcccggca gatttatccg
     1921 tgggtcagct ggctgtacca ggagtattac ggtctggccg gggctttcct agttatcggc
     1981 ctgtttttcg gtctggctga actttgggcc ggacggatgc gttttgtggg gctgagtcat
     2041 aaggagcgac gggagtatgc cgatcgctat aagcgcgata ataaacggcc agcgccattt
     2101 tcggtgctgg cctcgctgga ttcgcccggg cggatggtca gggcacgtcg acatgtctgg
     2161 aactacgcgt actggctgac cggcgcggcc ggtctggccc ttctgggggg gctggttgtc
     2221 gccgtgttta tcgagagctg gggagaaacc gtcctgcgtc cggacctcta cgcaaacgca
     2281 gattattacc tgctgcagcc ggctaacctg ttcgggattg cctggtgcgc gggcgggctg
     2341 gctgtcggga ttgtgttggc gcggctgctg gcgttcctgc tgcttgacgg tcattttgcg
     2401 gctgctgacg aggcggtgaa cgcgaaactg aatcaggaga gccggcgcag cagccagcgg
     2461 acaggagaga tgaccgacgt gcgccacctg catttcgggg agccggtacc ggttaatgcg
     2521 ctggccgatt tttcctcgga gcaggcccgg caggagcagg cggtgtttct gggtaaggat
     2581 gagcagggca agccggtgct ggttccgcgt gatacctggc gcaagaccaa cattcagatc
     2641 ctcggcctgc ccgggagcgg caagagcgtg atggccacca acgcgctcat tcgctgcgta
     2701 cgcgatttcg gggacgcggt ggtgtacttc gaccccaaag gggacgcatg ggcgccgcac
     2761 gtcttccggg cgcactgtcc ggacttcacc ctgctggatc tgcggccggg gaaaccggcg
     2821 cagctgaatc tgttccgcga tctcgaccag tacgcgctga aaaacctgct gattgccggg
     2881 cttaacctgg gcgagaccgg cgacgtggcg gaccactacc gcatcagcga gcagaaagcg
     2941 gcgaagctga ttgcggagca gtttccgcag ggggcaaaca ttcagcaggt gctggcggcg
     3001 gcgtacgcgc tgccggaggc cctgaaaaag gacgtgaagg ggttgatcac taggctggaa
     3061 aacgtcgctg acctgagcgt cctgcagacg gacagtggca tcgatgtggc ggggattatc
     3121 aacggcggcg gctgcctgta cgttatcggc tcgatggacg acgaggcggt gatccgtgtg
     3181 caaaaaatgc tgtttgcccg ctgcgcgcaa atcatcattg cgcgggacga gttccgggag
     3241 tggccacacg tcagcgtgat gctcgatgaa attaaatatc tgctgtcgaa gtatgtactg
     3301 aacgcactgg ggacgctgcg cagccgggac tgcaatctgt tgctcgcgca ccagtcgctg
     3361 ggcgatttcg ggcagtgcgg gcaggatctg cccgctgaat ttgtgaaaac cacggtactg
     3421 gataacacgc cgatccgctg gttttaccgg gcggccagcc tggagtccgc gcagtgggcg
     3481 gcggggcaga ccggggaaat cagggtggac gtggagcgcc gccgtgccag ccgggaggcg
     3541 gggaacgtgg agcatatcag cggggacagc ttcatccaga aggaggcgcg gccgctgttt
     3601 gacgtgaaca ccctgcaaca cctgccggac gggttcgcgg tcatgaccgg gctgggtgtg
     3661 gcccggatgg cgttcagcag tccgctgcgg gttgagcgcc gggaaatccc gttgcagcgt
     3721 ttcccggtgc tggcgaaaac cgatcccctg gcggagttcc ggcccccggc cgacggcggc
     3781 gggccgaaag cggatgatga ttttgcgggg atttactgat ggcgctggag ctgtacaacg
     3841 tcagccacgc gaaacgacag gcgcggaacg cggagaaaac ccggctgacc ctgcgctggt
     3901 tgcgggaaga gctgtgttcc acggcggagc tggttgcgcg gcggctgggg attgcggcgg
     3961 tgcagccggt ctaccgcttc ctcgacagcc tggtggcgaa agggctgctg gtccggacca
     4021 aatatccggt ggacggccgt caggtcagcg tgtgggggct gacgccgcat ggcgtggcgt
     4081 tctcgttcga cgaggacgaa ccgctggcgg atgttattcc gtttcagccg tcaagggtgt
     4141 cggcagcaca tctgccgcac cggctggccg tgcaatccct gcgccttgat atggaagcca
     4201 gaggggccac cggctggcgc attctgcacc gtctgagtct taaaggcatg aaagtgccgg
     4261 acgccctggc ggagctggac ggtaaaacgg tggcgtttga ggtggagcgc accgtcaaga
     4321 gccgtcgccg gtatcaggaa gtggtgtccg ggtatctgtt caaccgcaaa gctaacggca
     4381 tcgatgaaat ctggtacatc tgcccggacc gggcgacgca ggtcagggtg cagcgggcaa
     4441 tcctgtcggt ggatgaaatc gtgaacccac agaccggcga ggcccgcaaa acggcagagc
     4501 tggatcgtga acggttgttt gcgtgcttta aatttatgac aacggagtga gggattatgg
     4561 cgctgtacat ggtatttatt gaagacgcgg aggaccggaa cggcgagcgc tgcatcatgc
     4621 cgaccagcga gcatctggaa gatatggtgg tgctcgcctg gcagaccggg gcgtatgttt
     4681 tctgttttgt cgaccgggcc cagcatctgg cgatgatgaa atccatcggg atgtatgacg
     4741 agcggctcaa cagcgacgaa aaagccacgt actttttcac tggtaaaaat ccgcaatggg
     4801 aacggaacaa acccacagga atgaagctgt gtgattacct ctatcgcaag gtggagcggt
     4861 tcgccatcga gaacggtgtg caggtggttg atgccgggaa gtgctcaccg gagcagatta
     4921 acgcgatgat tgacgtggtg agtgagcggc acggggaaag cgcggtgaac gagaagctga
     4981 aagcggagct gatgcgggaa tataacagcc gctattttaa cgtgccggta cctgaactgc
     5041 cggaggacaa acctgcccgc cggtggtggc cgttcggcaa aaaataaatg ccagcaccgg
     5101 ccatgccggt gctgttattc ctccccgagc ttatcccgca tcacctgcca gagtgtttcc
     5161 gcatcctcgt gtagccgctc gcacatgtcg gccggggtat ccatatccag ctcattcagt
     5221 ttatcgagca atagcagcga ctgctcctta ataaatttag ccatattgag ggctgttttt
     5281 tcctgttttg tcatgattct ttccttccgg gcatggtgtg cgcctgtcag gtatactgtc
     5341 tcaaaaatag cggggtgaga caatggcgtg ggtgacggtg agacaggcga cggaactgac
     5401 gggtaaagcc cgaagttccc tgtatcggga tatggcgaaa ggccgcgtat cataccgtac
     5461 ggaagctgat ggcggccggg tggtggatac cagcgagctt atccgggtgt acggtgagtt
     5521 aagacaggat gagactcacg agcgggacga gttgagactg tcgggtgaga ctgaaaaaat
     5581 ggcctccgac gcgttgatcg cggagataaa agcactcagg gaggaggtgg ccgggctacg
     5641 gcaggaaatg cagtccatga gactgctgga acacaaaccg gagacagcag agaccccggc
     5701 cggaacaccg cgccggtggt ggccgtgggg aaaacgctga cgcgctgtgc ttgtccaacc
     5761 ggcgacggcc acgcaatgaa ttgcgtcccc gtcaccggtt attagagaac aatatgggga
     5821 ttctggcgct ggccgaccac ggccagaacc cggacttttc tggtctggtc atcaacatca
     5881 tagaggacat gctgtcccat cagactgatc cggcgaacgt cctcgccata gcgtgagtcc
     5941 acttcataga gtcgcgggaa gtcggccagt ttgtccgccg cagcgagaac ccggtcggta
     6001 acgttctgcg catcccacag ggatgaattt tccgcgatat agtcaaacag cgtcgttaca
     6061 tcctcgatgg ctccctgctt ccattccacg tcatacggca tcgcgcagtt cctgataacg
     6121 gccatgcagg cgctggcgga gttgttccgt ggtcataccc ggacgcgtgt cagcacgggc
     6181 ggcggtcact ttggcgcgaa cctgatcttc atactctgcc tgggccagtt cttcgttagc
     6241 cagtcgctgc aacagcgcag ctttgtccgg gccaccttcc ggcagtatgc ggggcaggct
     6301 ttcagccagt tcccgaagaa agttggcgtt ttcgtagtgt gtagtgttgc tcattgcgga
     6361 gcctcccggg ttaatgcggc aatatgaagg aagtttagca gggggcggtc tgaaagaata
     6421 tggtcgtgtc agtcaggctg atgaagcaga aagtcggtaa aaaagggctt acggcatcca
     6481 ttttacgtca aaacatatgc catgcagttg cgtgcatagg catgcattag gataaaattt
     6541 atcgggcgcg tttccggcag ttttccgggg gggttgttgc ctgttttatc ccgtagccgc
     6601 cggaaacgcc ctgagcccgt ctgagcggtg cgcgtaatga cgcgttatgg taaatccact
     6661 tactgtctgc cttcatagcc atcgagataa acagctgaaa tggtgtcagc cagtagcctt
     6721 gattttttcg gtgtgaggtc tttgctgcgg taatcggtac ccgcaaattg tgttgtgcct
     6781 tggagtgagt ttaaaaattc ctgactgtga aactgctgaa tgcgttgctg cacatcactc
     6841 cgtgattttc cggtcgcttt atcctttgcg cccatatccc agaactgccg gaaaaaaggc
     6901 accatcattc gtgttgtgtc agctttctgg gtggaaaaat tgggatcaaa aggggtctga
     6961 cgctcagtgg aacgaaaact cacgttaagc aacgttttct gcctctgacg cctcttttaa
     7021 tggtctcaga tgacctttgg tcaccagttc tgccagcgtg aaggaataat ggccgagcat
     7081 attgatatgt ccgtggcaaa gcggggagag gcgtgcgata tcttcatcat tcagtgtttc
     7141 accctgcgcc cggagatgat ccagagctgc ctgcatataa atagtgttcc ataacacgac
     7201 ggcgttagtg accagcccca gagctcccaa ctgatcttcc tgaccgtcgg tatatcgttt
     7261 tcttatctca cctttttgac cgtgacagat ggctctggca actgcatgac ggctttctcc
     7321 ccgattaagc tgggtcagaa tgcgccggcg gtaatcttca tcatcaatat aattaagcag
     7381 atacagcgtt ttgttgatgc gccccacttc aatgattgcc tgagtcagtc cggagggacg
     7441 ttcacttttc agcaatgaac ggaccagcac tgaggcctgt actttgccca gcttcaggga
     7501 gcctgcggtc cggatcattt cgtcccactg aaggactatt tttcggggat ctgattgccc
     7561 tctggcaata tcattcagca cgccatagtc ggcatcatgg tccattcgcc agaaaaccga
     7621 agcaccggca tcagccaggc gtggagaaaa ctggtatccc agcagccaga aaaggccaaa
     7681 gacaagatcg ctggcacctg ccgtatcggt cataatttcg gttggattca gcccggtctc
     7741 ctgttccaga aggccttcca gcacaaagat agagtccctc agcgtccccg gtataacgat
     7801 gccatgaaag ccggaatact gatcggacac aaagttgtac caggtgatcc ctctgttatt
     7861 accaaagtat ttgcggttcg gtccggcatt gattgttctg actggcgtaa caaagcgcat
     7921 tccatctgca gatgccactt ctcctccacc ccatatctgt gccagtggca gcgttgcctg
     7981 aaaatcaacc agtctggcat tagcgctggt gatagtttca gcccgcagat agttcgcttt
     8041 tgtccagttc agccggtgtc gggtcagtgc aggaacattt gatctgatca gtggttccag
     8101 accgatattg caggcttcag ccatcagcac ggcgctgatg ctgacgggca gatcatcaac
     8161 tctggcactg gcttcactgg catggaaaaa ctcatcagca aatccggtat gggcgttaat
     8221 ttcgagcagc aactccgtta aatccaccgg agggagcaga tcactgatca ttttgctcag
     8281 tcgtttcaga ctgtccggct catcaagact ggcgagggga gaaattgtca accggggctt
     8341 cgggccagaa acatcgagtt cgacagcctc attttcgcca agacgtgcag caacctgtct
     8401 gtaacgacta tcaagctgat ggcccagaga ttttattgct tcctgcgggt ctgtcgggtg
     8461 ccccaaagaa cgataaacct taatccgatt tgcctgccag tcagcaccct gtagtaatct
     8521 tgcacgagga tctccccacc ggttactgcc ggtaacgtag acatccctcc gtctcagact
     8581 atcctgcagt ttactgagaa agcagagcgt gtatcccctt cgggtgatat gtttttcctt
     8641 gttaatcacc agccgtttcc atgactgact gatgatttcc gttggtgcgt cgtcaaaaaa
     8701 ctgccgccgt gagctgaact cccggctgag gtagtcacag gcattcagag tggtaacccc
     8761 ggcaggtgcg gatgaaaatt taacggtatt cagcagatgg ggcaggaaac gacgaacgcg
     8821 cccgtactgc tccaccattt cgtcatgaaa attatcgtct gagggccggg caatttcacg
     8881 gacaagcgtg atgatttcag ccagcttttg cctagggatg tagctgaaca cctcagcacg
     8941 aatcgattcg tccggtgttt cttctttcag caagtacgaa catgcgctgg cgagcgccaa
     9001 tgcagattta tccagatcct tcagcgagcg gagccgtttt ttctgcccaa tctttctggc
     9061 gtcacggatg ataacggcca gcatggcgtc cagaacgtcc agtgcatcat ccagcgccag
     9121 cgtttcccat gcaaggacaa aggcaaccag aaccgccatc cttttctgcg gtgacatcct
     9181 ggcaatattg aacaccgaag tcataccagc ataacgtgcg agatttttca ggcgcacagc
     9241 cgggagtgta ctcaggtttt cagcatgcag gccaaaatcg ttcagagttt tccagcgttc
     9301 aattgcttca ttaaacgccg gaccactgat ggtcacaggg ccttttttca gtgattccag
     9361 taaagacagg cggctgcaat cagttggccc cagcagcatc tccagctgtg aacgctgttc
     9421 ggctgacggt atcagtgcca gtttgttcca caggcgcaac gtcgcctttt cccttacctc
     9481 tgaaatcaac cgggtcagcg tggtggctcc ggggagaata atacgatgtt gcataagcca
     9541 ccctgtcgcc aggtcgaaaa gcaggccagg acgttcgttg cttatccagc tccgggtata
     9601 taaaagacgg gtaaggcgaa atgtccaggg ccaggcaaat tcacgatact gatagtgctg
     9661 acgtatcagc gctgcatgct cacggcgggt attttccctc tgaccgtatt ctgcaagaac
     9721 ggtgatatca cgaatcccga gctgtctggc ggtaaaatgc cggacgccgg aaggaatatg
     9781 attcatgtca gtaagaaaag tgcccagaaa acgcacacac ccgatttgca gggcaatacc
     9841 aagtcgattg tgatcacccc ggcttttccc gataaattct ttatccgctt catcaagatg
     9901 aaaataacgc gccagctgaa gttcatcggg ttcgccagtg aacctgccat aactctcaac
     9961 ctgctcagtg gtcaaaaaat caacgggcat atcggcctcc ctgcctgacg gcttttttaa
    10021 cacaactgca accgttcgaa atattataaa ttatcagaca tagtaaaacg gcttcgtttg
    10081 agtgtccatt aaatcgtcat tttggcataa tagacacatc gtgtctgata ttcgatttaa
    10141 ggtacatttt tatgcgaatt tttggttatg cgcgggtctc aaccagccag cagtccctcg
    10201 atattcagat cagagcgctc aaagatgcag gggtaaaagc taaccgcatc tttaccgaca
    10261 aggcatccgg cagttcaaca gatcgggaag ggctggattt gctgaggatg aaggtggagg
    10321 aaggtgatgt cattctggtg aagaagctcg accgtcttgg ccgcgacacc gccgacatga
    10381 tccaactgat aaaagagttt gatgctcagg gtgtagcggt tcggtttatt gacgacggga
    10441 tcagtaccga cggtgatatg gggcaaatgg tggtcaccat cctgtcggct gtggcacagg
    10501 ctgaacgccg gaggatccta gagcgcacga atgagggccg acaggaagca aagctgaaag
    10561 gaatcaaatt tggccgcagg cgtaccgtgg acaggaacgt cgtgctgacg cttcatcaga
    10621 agggcactgg tgcaacggaa attgctcatc agctcagtat tgcccgctcc acggtttata
    10681 aaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
    10741 aataatggtt tcttagacgt caggtggcac ttttcgggga aaagtgcgcg gaacccctat
    10801 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    10861 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc aaacaaagtt
    10921 aggcatcaca aagtacagca tcgtgaccaa cagcaacgat tccgtcacac tgcgcctcat
    10981 gactgagcat gaccttgcga tgctctatga gtggctaaat cgatctcata tcgtcgagtg
    11041 gtggggcgga gaagaagcac gcccgacact tgctgacgta caggaacagt acttgccaag
    11101 cgttttagcg caagagtccg tcactccata cattgcaatg ctgaatggag agccgattgg
    11161 gtatgcccag tcgtacgttg ctcttggaag cggggacgga tggtgggaag aagaaaccga
    11221 tccaggagta cgcggaatag accagttact ggcgaatgca tcacaactgg gcaaaggctt
    11281 gggaaccaag ctggttcgag ctctggttga gttgctgttc aatgatcccg aggtcaccaa
    11341 gatccaaacg gacccgtcgc cgagcaactt gcgagcgatc cgatgctacg agaaagcggg
    11401 gtttgagagg caaggtaccg taaccacccc agatggtcca gccgtgtaca tggttcaaac
    11461 acgccaggca ttcgagcgaa cacgcagtgt tgcctaaccc ttccatcgag ggggacgtcc
    11521 aagggctggc gcccttggcc gcccctcatg tcaaacgtta aacatcatga gggaagcggt
    11581 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
    11641 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
    11701 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
    11761 tttgatcaac gaccttttgg aaacggcact gttgcaaata gtcggtggtg ataaacttat
    11821 catccccttt tgctgatgga gctgcacatg aacccattca aaggccggca ttttcagcgt
    11881 gacatcattc ggtgggccgt acgctggtac tgcaaatacg gcatcagtta ccgtgagctg
    11941 caggagatgc tggctgaacg cggagtgaat gtcgatcact ccacgattta ccgctgggtt
    12001 cagcgttatg cgcctgaaat ggaaaaacgg ctgcgctggt actggcgtaa cccttccgat
    12061 ctttgcccgt ggcacatgga tgaaacctac gtgaaggtca atggccgctg ggcgtatctg
    12121 taccgggccg tcgacagccg gggccgcact gtcgattttt atctctcctc ccgtcgtaac
    12181 agcaaagctg cataccggtt tctgggtaaa atcctcaaca acgtgaagaa gtggcagatc
    12241 ccgcgattca tcaacacgga taaagcgccc gcctatggtc gcgcgcttgc tctgctcaaa
    12301 cgcgaaggcc ggtgcccgtc tgacgttgaa caccgacaga ttaagtaccg gaacaacgtg
    12361 attgaatgcg atcatggcaa actgaaacgg ataatcggcg ccacgctggg atttaaatcc
    12421 atgaagacgg cttacgccac catcaaaggt attgaggtga tgcgtgcact acgcaaaggc
    12481 caggcctcag cattttatta tggtgatccc ctgggcgaaa tgcgcctggt aagcagagtt
    12541 tttgaaatgt aaggcctttg aataagacaa aaggctgcct catcgctaac tttgcaacag
    12601 tgccctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    12661 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    12721 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    12781 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    12841 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    12901 tcgttccact gagcgtcaga cccctaattc aggtctggga cacaatggtg gtcaggccag
    12961 tgggaatgtg aacggtacat caggtaaagg tggcccttca tcaggtggtg gtacggatcc
    13021 aaacagcggg ccgggctggg gtacgacgca tacacctaac ggggatattc ataactataa
    13081 cccgggggag tttggtaacg gcgggagtaa acccggtggt aatggcggta acagcggcaa
    13141 tcatagcggg agctctggtg gcggacagtc ttcggccacc gcgatggcct tcggtctgcc
    13201 tgccttggcc actccggggg ctgaaggact ggctttatcc gtttccggcg atgcgttgtc
    13261 ggccgctgtt gctgatgtgc tggctgccct gaaaggtccg tttaagtttg gtctgtgggg
    13321 gattgcgatt tacggagtgc tgccttctga gatagcaaaa gatgatccga aaatgatgtc
    13381 aaaaatcgtg acgtcattgc cggcagacac ggtaacggag acgccggtca gctccctgcc
    13441 gctggaccag gcgacggtca gcgtcactaa acgtgtggcg gatattgtga aggacgagcg
    13501 gcagcatatt gcggttgtca ccggccggcc aatgagtgtt cctgtggtgg atgcgaaacc
    13561 gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt ctcccgtctc tgcaggtgag
    13621 cgtacctaaa ggtgttccgg ccgcgaaagc cccgccaaaa ggcattattg ctgaaaaagg
    13681 tgattcacgt ccggctggtt ttacggccgg tggtaactcc cgtgaagccg ttattcgttt
    13741 cccgaaagag agcggacaga aaccggtcta tgtgtcggtg acggatgttc ttactccggc
    13801 acaggtaaaa cagcgtctgg aggaagaaaa gcgtcgccag caggcatggg acgctgctca
    13861 cccggaagag gggctgaaaa gagagtatga taaagcgaaa gctgagctgg atgctgaaga
    13921 taaaaatatt gcgaccttaa acagtcgcat tgcatcgaca gagaaggcga tccccggtgc
    13981 aagggctgct gttcaggaag ccgataaaaa ggtaaaagag gcagaggcaa ataaagatga
    14041 ttttgtgact tataacccgc ctcatgaata tggctccggg tggcaggatc aggttcgcta
    14101 tcttgataag gatattcaga atcagaatga gaaattaaaa gctgcccaga catctttaaa
    14161 cgaaatgaat gaatccttat ccagggataa ggctgcgctt tccggggcga tggagagccg
    14221 gaaacaaaag gagaaaaaag cgaaggatgc agaaaataaa ttaaatgagg aaaagaaaaa
    14281 acctcgtaag ggaactaaag attacggcca tgattatttt cctgatccca agactgaaga
    14341 tattaagggg ttgggagagt tgaaagaggg taaacctaaa acccctaaac aaggtggtgg
    14401 tggtaagcgg gcccgatggt atggtgataa aaagcgtaaa atttatgaat gggattccca
    14461 gcacggtgag cttgaagggt accgcgccag tgatggcgaa cacctcgggg cattcgatcc
    14521 aaaaacgggt aagcaggtta aagggccgga tccaaaacga aacattaaaa aatatcttta
    14581 agaggtaaat atggggctta aattacatat tcattggttt gataagaaaa ccgaagagtt
    14641 taaaggtggt gaatactcaa aagacttcgg tgatgatggt tctgtcattg aaagtctggg
    14701 gatgcctt
//
