LOCUS       OW848861               13636 bp    DNA     circular BCT 17-MAY-2022
DEFINITION  Klebsiella pneumoniae isolate 512 genome assembly, plasmid: P3.
ACCESSION   OW848861
VERSION     OW848861.1
DBLINK      BioProject: PRJEB42440
            BioSample: SAMEA7853224
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2022) CNAG-CRG, CNAG, Spain
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="512"
                     /isolation_source="Hospital Universitario Gregorio Maranon
                     (Madrid)"
                     /db_xref="taxon:573"
                     /plasmid="P3"
     gene            <4..300
                     /locus_tag="AI2588V1_5442"
     mRNA            4..300
                     /locus_tag="AI2588V1_5442"
                     /note="AI2588v1_5442.mrna"
     CDS             <4..300
                     /locus_tag="AI2588V1_5442"
                     /note="short variant; AI2588v1_5442.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3518877.1"
                     /translation="VPDALAELDGKTVAFEVERTVKSRRRYQEVVSGYLFNRKANGIDE
                     IWYICPDRATQVRVQRAILSVDEIVNPQTGEARKTAELDRERLFACFKFMTTE"
                     /name="hypothetical protein"
     exon            4..300
                     /locus_tag="AI2588V1_5442"
                     /note="AI2588v1_5442.mrna"
                     /number=1
     gene            307..837
                     /locus_tag="AI2588V1_5443"
     mRNA            307..837
                     /locus_tag="AI2588V1_5443"
                     /note="AI2588v1_5443.mrna"
     CDS             307..837
                     /locus_tag="AI2588V1_5443"
                     /note="long variant; AI2588v1_5443.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3518931.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     exon            307..837
                     /locus_tag="AI2588V1_5443"
                     /note="AI2588v1_5443.mrna"
                     /number=1
     gene            complement(865..1044)
                     /locus_tag="AI2588V1_5444"
     mRNA            complement(865..1044)
                     /locus_tag="AI2588V1_5444"
                     /note="AI2588v1_5444.mrna"
     CDS             complement(865..1044)
                     /locus_tag="AI2588V1_5444"
                     /note="long variant; AI2588v1_5444.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Regulatory protein rop"
                     /protein_id="CAH3519043.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Regulatory protein rop"
     exon            complement(865..1044)
                     /locus_tag="AI2588V1_5444"
                     /note="AI2588v1_5444.mrna"
                     /number=1
     gene            1113..1490
                     /locus_tag="AI2588V1_5445"
     mRNA            1113..1490
                     /locus_tag="AI2588V1_5445"
                     /note="AI2588v1_5445.mrna"
     CDS             1113..1490
                     /locus_tag="AI2588V1_5445"
                     /note="long variant; AI2588v1_5445.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3519157.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     exon            1113..1490
                     /locus_tag="AI2588V1_5445"
                     /note="AI2588v1_5445.mrna"
                     /number=1
     gene            complement(1552..1851)
                     /locus_tag="AI2588V1_5446"
     mRNA            complement(1552..1851)
                     /locus_tag="AI2588V1_5446"
                     /note="AI2588v1_5446.mrna"
     CDS             complement(1552..1851)
                     /locus_tag="AI2588V1_5446"
                     /note="long variant; AI2588v1_5446.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3519269.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="hypothetical protein"
     exon            complement(1552..1851)
                     /locus_tag="AI2588V1_5446"
                     /note="AI2588v1_5446.mrna"
                     /number=1
     gene            complement(1841..2104)
                     /locus_tag="AI2588V1_5447"
     mRNA            complement(1841..2104)
                     /locus_tag="AI2588V1_5447"
                     /note="AI2588v1_5447.mrna"
     CDS             complement(1841..2104)
                     /locus_tag="AI2588V1_5447"
                     /note="long variant; AI2588v1_5447.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3519384.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     exon            complement(1841..2104)
                     /locus_tag="AI2588V1_5447"
                     /note="AI2588v1_5447.mrna"
                     /number=1
     gene            complement(2410..>2742)
                     /locus_tag="AI2588V1_5448"
     mRNA            complement(2410..2742)
                     /locus_tag="AI2588V1_5448"
                     /note="AI2588v1_5448.mrna"
     CDS             complement(2410..>2742)
                     /locus_tag="AI2588V1_5448"
                     /note="short variant; AI2588v1_5448.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3519493.1"
                     /translation="LLNVSFRSTERQTPFDPNFSTQKADTTRMMVPFFRQFWDMGAKDK
                     ATGKSRSDVQQRIQQFHSQEFLNSLQGTTQFAGTDYRSKDLTPKKSRLLADTISAVYLD
                     GYEGRQ"
                     /name="hypothetical protein"
     exon            complement(2410..2742)
                     /locus_tag="AI2588V1_5448"
                     /note="AI2588v1_5448.mrna"
                     /number=1
     gene            complement(2735..5740)
                     /locus_tag="AI2588V1_5449"
     mRNA            complement(2735..5740)
                     /locus_tag="AI2588V1_5449"
                     /note="AI2588v1_5449.mrna"
     CDS             complement(2735..5740)
                     /locus_tag="AI2588V1_5449"
                     /note="long variant; AI2588v1_5449.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3519607.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="hypothetical protein"
     exon            complement(2735..5740)
                     /locus_tag="AI2588V1_5449"
                     /note="AI2588v1_5449.mrna"
                     /number=1
     gene            5902..6459
                     /locus_tag="AI2588V1_5450"
     mRNA            5902..6459
                     /locus_tag="AI2588V1_5450"
                     /note="AI2588v1_5450.mrna"
     CDS             5902..6459
                     /locus_tag="AI2588V1_5450"
                     /note="long variant; AI2588v1_5450.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Transposon Tn3 resolvase"
                     /protein_id="CAH3519717.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="Transposon Tn3 resolvase"
     exon            5902..6459
                     /locus_tag="AI2588V1_5450"
                     /note="AI2588v1_5450.mrna"
                     /number=1
     gene            6642..7247
                     /locus_tag="AI2588V1_5451"
     mRNA            6642..7247
                     /locus_tag="AI2588V1_5451"
                     /note="AI2588v1_5451.mrna"
     CDS             6642..7247
                     /locus_tag="AI2588V1_5451"
                     /note="long variant; AI2588v1_5451.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside N(6')-acetyltransferase type 1"
                     /protein_id="CAH3519831.1"
                     /translation="MSIQHFQTKLGITKYSIVTNSNDSVTLRLMTEHDLAMLYEWLNRS
                     HIVEWWGGEEARPTLADVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGW
                     WEEETDPGVRGIDQLLANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAI
                     RCYEKAGFERQGTVTTPDGPAVYMVQTRQAFERTRSVA"
                     /name="Aminoglycoside N(6')-acetyltransferase type 1"
     exon            6642..7247
                     /locus_tag="AI2588V1_5451"
                     /note="AI2588v1_5451.mrna"
                     /number=1
     gene            <7329..7586
                     /locus_tag="AI2588V1_5452"
     mRNA            7329..7586
                     /locus_tag="AI2588V1_5452"
                     /note="AI2588v1_5452.mrna"
     CDS             <7329..7586
                     /locus_tag="AI2588V1_5452"
                     /note="short variant; AI2588v1_5452.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptomycin 3''-adenylyltransferase"
                     /protein_id="CAH3519945.1"
                     /translation="VIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDID
                     LLVTVTVRLDETTRRALINDLLETALLQIVGGDKLIIPFC"
                     /name="Streptomycin 3''-adenylyltransferase"
     exon            7329..7586
                     /locus_tag="AI2588V1_5452"
                     /note="AI2588v1_5452.mrna"
                     /number=1
     gene            complement(7534..8353)
                     /locus_tag="AI2588V1_REPA000000169"
     repeat_region   complement(7534..8353)
                     /locus_tag="AI2588V1_REPA000000169"
                     /note="IS15DIV_IS6_unknown"
                     /rpt_family="IS"
     gene            7598..8302
                     /locus_tag="AI2588V1_5453"
     mRNA            7598..8302
                     /locus_tag="AI2588V1_5453"
                     /note="AI2588v1_5453.mrna"
     CDS             7598..8302
                     /locus_tag="AI2588V1_5453"
                     /note="long variant; AI2588v1_5453.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3520069.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="hypothetical protein"
     exon            7598..8302
                     /locus_tag="AI2588V1_5453"
                     /note="AI2588v1_5453.mrna"
                     /number=1
     gene            <8723..9259
                     /locus_tag="AI2588V1_5454"
     mRNA            8723..9259
                     /locus_tag="AI2588V1_5454"
                     /note="AI2588v1_5454.mrna"
     CDS             <8723..9259
                     /locus_tag="AI2588V1_5454"
                     /note="short variant; AI2588v1_5454.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Colicin-E3"
                     /protein_id="CAH3520180.1"
                     /translation="VTYNPPHEYGSGWQDQVRYLDKDIQNQNEKLKAAQTSLNEMNESL
                     SRDKAALSGAMESRKQKEKKAKDAENKLNEEKKKPRKGTKDYGHDYFPDPKTEDIKGLG
                     ELKEGKPKTPKQGGGGKRARWYGDKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGK
                     QVKGPDPKRNIKKYL"
                     /name="Colicin-E3"
     exon            8723..9259
                     /locus_tag="AI2588V1_5454"
                     /note="AI2588v1_5454.mrna"
                     /number=1
     gene            9269..9526
                     /locus_tag="AI2588V1_5455"
     mRNA            9269..9526
                     /locus_tag="AI2588V1_5455"
                     /note="AI2588v1_5455.mrna"
     CDS             9269..9526
                     /locus_tag="AI2588V1_5455"
                     /note="long variant; AI2588v1_5455.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="Colicin-E3 immunity protein"
                     /protein_id="CAH3520291.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="Colicin-E3 immunity protein"
     exon            9269..9526
                     /locus_tag="AI2588V1_5455"
                     /note="AI2588v1_5455.mrna"
                     /number=1
     gene            10401..10529
                     /locus_tag="AI2588V1_5456"
     mRNA            10401..10529
                     /locus_tag="AI2588V1_5456"
                     /note="AI2588v1_5456.mrna"
     CDS             10401..10529
                     /locus_tag="AI2588V1_5456"
                     /note="long variant; AI2588v1_5456.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3520408.1"
                     /translation="MEKRHLRGLAVLPHMLVPCLFTESVGNFACVRSARRSLTAGA"
                     /name="hypothetical protein"
     exon            10401..10529
                     /locus_tag="AI2588V1_5456"
                     /note="AI2588v1_5456.mrna"
                     /number=1
     gene            11241..13205
                     /locus_tag="AI2588V1_5457"
     mRNA            11241..13205
                     /locus_tag="AI2588V1_5457"
                     /note="AI2588v1_5457.mrna"
     CDS             11241..13205
                     /locus_tag="AI2588V1_5457"
                     /note="long variant; AI2588v1_5457.mrna"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="CAH3520503.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="hypothetical protein"
     exon            11241..13205
                     /locus_tag="AI2588V1_5457"
                     /note="AI2588v1_5457.mrna"
                     /number=1
     ARG             6694..7244
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7330..7586
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             6643..7247
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     Typing          complement(9864..10442)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..297
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.65e-58"
ORIGIN
        1 aaagtgccgg acgccctggc ggagctggac ggtaaaacgg tggcgtttga ggtggagcgc
       61 accgtcaaga gccgtcgccg gtatcaggaa gtggtgtccg ggtatctgtt caaccgcaaa
      121 gctaacggca tcgatgaaat ctggtacatc tgcccggacc gggcgacgca ggtcagggtg
      181 cagcgggcaa tcctgtcggt ggatgaaatc gtgaacccac agaccggcga ggcccgcaaa
      241 acggcagagc tggatcgtga acggttgttt gcgtgcttta aatttatgac aacggagtga
      301 gggattatgg cgctgtacat ggtatttatt gaagacgcgg aggaccggaa cggcgagcgc
      361 tgcatcatgc cgaccagcga gcatctggaa gatatggtgg tgctcgcctg gcagaccggg
      421 gcgtatgttt tctgttttgt cgaccgggcc cagcatctgg cgatgatgaa atccatcggg
      481 atgtatgacg agcggctcaa cagcgacgaa aaagccacgt actttttcac tggtaaaaat
      541 ccgcaatggg aacggaacaa acccacagga atgaagctgt gtgattacct ctatcgcaag
      601 gtggagcggt tcgccatcga gaacggtgtg caggtggttg atgccgggaa gtgctcaccg
      661 gagcagatta acgcgatgat tgacgtggtg agtgagcggc acggggaaag cgcggtgaac
      721 gagaagctga aagcggagct gatgcgggaa tataacagcc gctattttaa cgtgccggta
      781 cctgaactgc cggaggacaa acctgcccgc cggtggtggc cgttcggcaa aaaataaatg
      841 ccagcaccgg ccatgccggt gctgttattc ctccccgagc ttatcccgca tcacctgcca
      901 gagtgtttcc gcatcctcgt gtagccgctc gcacatgtcg gccggggtat ccatatccag
      961 ctcattcagt ttatcgagca atagcagcga ctgctcctta ataaatttag ccatattgag
     1021 ggctgttttt tcctgttttg tcatgattct ttccttccgg gcatggtgtg cgcctgtcag
     1081 gtatactgtc tcaaaaatag cggggtgaga caatggcgtg ggtgacggtg agacaggcga
     1141 cggaactgac gggtaaagcc cgaagttccc tgtatcggga tatggcgaaa ggccgcgtat
     1201 cataccgtac ggaagctgat ggcggccggg tggtggatac cagcgagctt atccgggtgt
     1261 acggtgagtt aagacaggat gagactcacg agcgggacga gttgagactg tcgggtgaga
     1321 ctgaaaaaat ggcctccgac gcgttgatcg cggagataaa agcactcagg gaggaggtgg
     1381 ccgggctacg gcaggaaatg cagtccatga gactgctgga acacaaaccg gagacagcag
     1441 agaccccggc cggaacaccg cgccggtggt ggccgtgggg aaaacgctga cgcgctgtgc
     1501 ttgtccaacc ggcgacggcc acgcaatgaa ttgcgtcccc gtcaccggtt attagagaac
     1561 aatatgggga ttctggcgct ggccgaccac ggccagaacc cggacttttc tggtctggtc
     1621 atcaacatca tagaggacat gctgtcccat cagactgatc cggcgaacgt cctcgccata
     1681 gcgtgagtcc acttcataga gtcgcgggaa gtcggccagt ttgtccgccg cagcgagaac
     1741 ccggtcggta acgttctgcg catcccacag ggatgaattt tccgcgatat agtcaaacag
     1801 cgtcgttaca tcctcgatgg ctccctgctt ccattccacg tcatacggca tcgcgcagtt
     1861 cctgataacg gccatgcagg cgctggcgga gttgttccgt ggtcataccc ggacgcgtgt
     1921 cagcacgggc ggcggtcact ttggcgcgaa cctgatcttc atactctgcc tgggccagtt
     1981 cttcgttagc cagtcgctgc aacagcgcag ctttgtccgg gccaccttcc ggcagtatgc
     2041 ggggcaggct ttcagccagt tcccgaagaa agttggcgtt ttcgtagtgt gtagtgttgc
     2101 tcattgcgga gcctcccggg ttaatgcggc aatatgaagg aagtttagca gggggcggtc
     2161 tgaaagaata tggtcgtgtc agtcaggctg atgaagcaga aagtcggtaa aaaagggctt
     2221 acggcatcca ttttacgtca aaacatatgc catgcagttg cgtgcatagg catgcattag
     2281 gataaaattt atcgggcgcg tttccggcag ttttccgggg gggttgttgc ctgttttatc
     2341 ccgtagccgc cggaaacgcc ctgagcccgt ctgagcggtg cgcgtaatga cgcgttatgg
     2401 taaatccact tactgtctgc cttcatagcc atcgagataa acagctgaaa tggtgtcagc
     2461 cagtagcctt gattttttcg gtgtgaggtc tttgctgcgg taatcggtac ccgcaaattg
     2521 tgttgtgcct tggagtgagt ttaaaaattc ctgactgtga aactgctgaa tgcgttgctg
     2581 cacatcactc cgtgattttc cggtcgcttt atcctttgcg cccatatccc agaactgccg
     2641 gaaaaaaggc accatcattc gtgttgtgtc agctttctgg gtggaaaaat tgggatcaaa
     2701 aggggtctga cgctcagtgg aacgaaaact cacgttaagc aacgttttct gcctctgacg
     2761 cctcttttaa tggtctcaga tgacctttgg tcaccagttc tgccagcgtg aaggaataat
     2821 ggccgagcat attgatatgt ccgtggcaaa gcggggagag gcgtgcgata tcttcatcat
     2881 tcagtgtttc accctgcgcc cggagatgat ccagagctgc ctgcatataa atagtgttcc
     2941 ataacacgac ggcgttagtg accagcccca gagctcccaa ctgatcttcc tgaccgtcgg
     3001 tatatcgttt tcttatctca cctttttgac cgtgacagat ggctctggca actgcatgac
     3061 ggctttctcc ccgattaagc tgggtcagaa tgcgccggcg gtaatcttca tcatcaatat
     3121 aattaagcag atacagcgtt ttgttgatgc gccccacttc aatgattgcc tgagtcagtc
     3181 cggagggacg ttcacttttc agcaatgaac ggaccagcac tgaggcctgt actttgccca
     3241 gcttcaggga gcctgcggtc cggatcattt cgtcccactg aaggactatt tttcggggat
     3301 ctgattgccc tctggcaata tcattcagca cgccatagtc ggcatcatgg tccattcgcc
     3361 agaaaaccga agcaccggca tcagccaggc gtggagaaaa ctggtatccc agcagccaga
     3421 aaaggccaaa gacaagatcg ctggcacctg ccgtatcggt cataatttcg gttggattca
     3481 gcccggtctc ctgttccaga aggccttcca gcacaaagat agagtccctc agcgtccccg
     3541 gtataacgat gccatgaaag ccggaatact gatcggacac aaagttgtac caggtgatcc
     3601 ctctgttatt accaaagtat ttgcggttcg gtccggcatt gattgttctg actggcgtaa
     3661 caaagcgcat tccatctgca gatgccactt ctcctccacc ccatatctgt gccagtggca
     3721 gcgttgcctg aaaatcaacc agtctggcat tagcgctggt gatagtttca gcccgcagat
     3781 agttcgcttt tgtccagttc agccggtgtc gggtcagtgc aggaacattt gatctgatca
     3841 gtggttccag accgatattg caggcttcag ccatcagcac ggcgctgatg ctgacgggca
     3901 gatcatcaac tctggcactg gcttcactgg catggaaaaa ctcatcagca aatccggtat
     3961 gggcgttaat ttcgagcagc aactccgtta aatccaccgg agggagcaga tcactgatca
     4021 ttttgctcag tcgtttcaga ctgtccggct catcaagact ggcgagggga gaaattgtca
     4081 accggggctt cgggccagaa acatcgagtt cgacagcctc attttcgcca agacgtgcag
     4141 caacctgtct gtaacgacta tcaagctgat ggcccagaga ttttattgct tcctgcgggt
     4201 ctgtcgggtg ccccaaagaa cgataaacct taatccgatt tgcctgccag tcagcaccct
     4261 gtagtaatct tgcacgagga tctccccacc ggttactgcc ggtaacgtag acatccctcc
     4321 gtctcagact atcctgcagt ttactgagaa agcagagcgt gtatcccctt cgggtgatat
     4381 gtttttcctt gttaatcacc agccgtttcc atgactgact gatgatttcc gttggtgcgt
     4441 cgtcaaaaaa ctgccgccgt gagctgaact cccggctgag gtagtcacag gcattcagag
     4501 tggtaacccc ggcaggtgcg gatgaaaatt taacggtatt cagcagatgg ggcaggaaac
     4561 gacgaacgcg cccgtactgc tccaccattt cgtcatgaaa attatcgtct gagggccggg
     4621 caatttcacg gacaagcgtg atgatttcag ccagcttttg cctagggatg tagctgaaca
     4681 cctcagcacg aatcgattcg tccggtgttt cttctttcag caagtacgaa catgcgctgg
     4741 cgagcgccaa tgcagattta tccagatcct tcagcgagcg gagccgtttt ttctgcccaa
     4801 tctttctggc gtcacggatg ataacggcca gcatggcgtc cagaacgtcc agtgcatcat
     4861 ccagcgccag cgtttcccat gcaaggacaa aggcaaccag aaccgccatc cttttctgcg
     4921 gtgacatcct ggcaatattg aacaccgaag tcataccagc ataacgtgcg agatttttca
     4981 ggcgcacagc cgggagtgta ctcaggtttt cagcatgcag gccaaaatcg ttcagagttt
     5041 tccagcgttc aattgcttca ttaaacgccg gaccactgat ggtcacaggg ccttttttca
     5101 gtgattccag taaagacagg cggctgcaat cagttggccc cagcagcatc tccagctgtg
     5161 aacgctgttc ggctgacggt atcagtgcca gtttgttcca caggcgcaac gtcgcctttt
     5221 cccttacctc tgaaatcaac cgggtcagcg tggtggctcc ggggagaata atacgatgtt
     5281 gcataagcca ccctgtcgcc aggtcgaaaa gcaggccagg acgttcgttg cttatccagc
     5341 tccgggtata taaaagacgg gtaaggcgaa atgtccaggg ccaggcaaat tcacgatact
     5401 gatagtgctg acgtatcagc gctgcatgct cacggcgggt attttccctc tgaccgtatt
     5461 ctgcaagaac ggtgatatca cgaatcccga gctgtctggc ggtaaaatgc cggacgccgg
     5521 aaggaatatg attcatgtca gtaagaaaag tgcccagaaa acgcacacac ccgatttgca
     5581 gggcaatacc aagtcgattg tgatcacccc ggcttttccc gataaattct ttatccgctt
     5641 catcaagatg aaaataacgc gccagctgaa gttcatcggg ttcgccagtg aacctgccat
     5701 aactctcaac ctgctcagtg gtcaaaaaat caacgggcat atcggcctcc ctgcctgacg
     5761 gcttttttaa cacaactgca accgttcgaa atattataaa ttatcagaca tagtaaaacg
     5821 gcttcgtttg agtgtccatt aaatcgtcat tttggcataa tagacacatc gtgtctgata
     5881 ttcgatttaa ggtacatttt tatgcgaatt tttggttatg cgcgggtctc aaccagccag
     5941 cagtccctcg atattcagat cagagcgctc aaagatgcag gggtaaaagc taaccgcatc
     6001 tttaccgaca aggcatccgg cagttcaaca gatcgggaag ggctggattt gctgaggatg
     6061 aaggtggagg aaggtgatgt cattctggtg aagaagctcg accgtcttgg ccgcgacacc
     6121 gccgacatga tccaactgat aaaagagttt gatgctcagg gtgtagcggt tcggtttatt
     6181 gacgacggga tcagtaccga cggtgatatg gggcaaatgg tggtcaccat cctgtcggct
     6241 gtggcacagg ctgaacgccg gaggatccta gagcgcacga atgagggccg acaggaagca
     6301 aagctgaaag gaatcaaatt tggccgcagg cgtaccgtgg acaggaacgt cgtgctgacg
     6361 cttcatcaga agggcactgg tgcaacggaa attgctcatc agctcagtat tgcccgctcc
     6421 acggtttata aaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6481 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aaagtgcgcg
     6541 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6601 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6661 aaacaaagtt aggcatcaca aagtacagca tcgtgaccaa cagcaacgat tccgtcacac
     6721 tgcgcctcat gactgagcat gaccttgcga tgctctatga gtggctaaat cgatctcata
     6781 tcgtcgagtg gtggggcgga gaagaagcac gcccgacact tgctgacgta caggaacagt
     6841 acttgccaag cgttttagcg caagagtccg tcactccata cattgcaatg ctgaatggag
     6901 agccgattgg gtatgcccag tcgtacgttg ctcttggaag cggggacgga tggtgggaag
     6961 aagaaaccga tccaggagta cgcggaatag accagttact ggcgaatgca tcacaactgg
     7021 gcaaaggctt gggaaccaag ctggttcgag ctctggttga gttgctgttc aatgatcccg
     7081 aggtcaccaa gatccaaacg gacccgtcgc cgagcaactt gcgagcgatc cgatgctacg
     7141 agaaagcggg gtttgagagg caaggtaccg taaccacccc agatggtcca gccgtgtaca
     7201 tggttcaaac acgccaggca ttcgagcgaa cacgcagtgt tgcctaaccc ttccatcgag
     7261 ggggacgtcc aagggctggc gcccttggcc gcccctcatg tcaaacgtta aacatcatga
     7321 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     7381 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     7441 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     7501 cgcggcgagc tttgatcaac gaccttttgg aaacggcact gttgcaaata gtcggtggtg
     7561 ataaacttat catccccttt tgctgatgga gctgcacatg aacccattca aaggccggca
     7621 ttttcagcgt gacatcattc tgtgggccgt acgctggtac tgcaaatacg gcatcagtta
     7681 ccgtgagctg caggagatgc tggctgaacg cggagtgaat gtcgatcact ccacgattta
     7741 ccgctgggtt cagcgttatg cgcctgaaat ggaaaaacgg ctgcgctggt actggcgtaa
     7801 cccttccgat ctttgcccgt ggcacatgga tgaaacctac gtgaaggtca atggccgctg
     7861 ggcgtatctg taccgggccg tcgacagccg gggccgcact gtcgattttt atctctcctc
     7921 ccgtcgtaac agcaaagctg cataccggtt tctgggtaaa atcctcaaca acgtgaagaa
     7981 gtggcagatc ccgcgattca tcaacacgga taaagcgccc gcctatggtc gcgcgcttgc
     8041 tctgctcaaa cgcgaaggcc ggtgcccgtc tgacgttgaa caccgacaga ttaagtaccg
     8101 gaacaacgtg attgaatgcg atcatggcaa actgaaacgg ataatcggcg ccacgctggg
     8161 atttaaatcc atgaagacgg cttacgccac catcaaaggt attgaggtga tgcgtgcact
     8221 acgcaaaggc caggcctcag cattttatta tggtgatccc ctgggcgaaa tgcgcctggt
     8281 aagcagagtt tttgaaatgt aaggcctttg aataagacaa aaggctgcct catcgctaac
     8341 tttgcaacag tgccctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8401 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8461 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8521 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8581 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8641 acgtgagttt tcgttccact gagcgtcaga ccccgaagcc gataaaaagg taaaagaggc
     8701 agaggcaaat aaagatgatt ttgtgactta taacccgcct catgaatatg gctccgggtg
     8761 gcaggatcag gttcgctatc ttgataagga tattcagaat cagaatgaga aattaaaagc
     8821 tgcccagaca tctttaaacg aaatgaatga atccttatcc agggataagg ctgcgctttc
     8881 cggggcgatg gagagccgga aacaaaagga gaaaaaagcg aaggatgcag aaaataaatt
     8941 aaatgaggaa aagaaaaaac ctcgtaaggg aactaaagat tacggccatg attattttcc
     9001 tgatcccaag actgaagata ttaaggggtt gggagagttg aaagagggta aacctaaaac
     9061 ccctaaacaa ggtggtggtg gtaagcgggc ccgatggtat ggtgataaaa agcgtaaaat
     9121 ttatgaatgg gattcccagc acggtgagct tgaagggtac cgcgccagtg atggcgaaca
     9181 cctcggggca ttcgatccaa aaacgggtaa gcaggttaaa gggccggatc caaaacgaaa
     9241 cattaaaaaa tatctttaag aggtaaatat ggggcttaaa ttacatattc attggtttga
     9301 taagaaaacc gaagagttta aaggtggtga atactcaaaa gacttcggtg atgatggttc
     9361 tgtcattgaa agtctgggga tgcctttaaa ggataatatt aataatggtt ggtttgatgt
     9421 tgaaaaatca tgggtttcga tattacagcc acactttaaa aatgtaatcg atattagtaa
     9481 atttgattac tttgtatcct ttgtttaccg ggatggtaac tggtaagcaa aagttatcaa
     9541 ggatgatttg aaatacagac ttccggtgtt cagggatgaa taccggggtt tttaaataca
     9601 gggaaggtat atgaaaaagg taaaggttgt ttttcttttt atattgattt tttcaggctt
     9661 tcttctggtc gcatgtcagg caaactatat ccgggatgtt cagggtggaa cggtggcacc
     9721 atcgtcctcc tctgaactga cggggatcgc ggttcagtag aaaagatcaa aggatcttct
     9781 tgagatcctt tttttctgcg cgtaatcttt tgccctgtaa acgaaaaaac cacctgggga
     9841 ggtggtttga tcgaaggtta agtcagttgg ggaactgctt aaccgtggta actggctttc
     9901 gcagagcaca gcaaccaaat ctgtccttcc agtgtagccg gactttggcg cacacttcaa
     9961 gagcaaccgc gtgtttagct aaacaaatcc tctgcgaact cccagttacc aatggctgct
    10021 gccagtggcg ttttgcgtgc ttttccgggt tggactcaag tgaacagtta ccggataagg
    10081 cgcagcagtc gggctgaacg gggggttctt gcttacagcc cagcttggag cgaacgacct
    10141 acaccgagcc gagataccag agtgtgagct atgagaaagc gccacacttc ccgcaaggga
    10201 gaaaggcgga acaggtatcc gggaaacggc agggttggaa caggagagcg caagagggag
    10261 cgattcatcg gaaacggtga tgatctttaa gtcctgtcgg gtttcgccac tcctgactga
    10321 ttcatggttg agccaccggc tcccacagat gcaccgaaaa agcgtctgtt tatgtgaact
    10381 cagtcaggag ggcggagcct atggaaaaac gccacctgcg tggccttgcc gttttgcctc
    10441 acatgttagt cccctgctta ttcacggaat ctgtgggtaa ctttgcatgt gtccgcagcg
    10501 cccgccgcag tctcacggcc ggagcgtagc gaccgagtga gcgaggaggc gcaatttccc
    10561 cggcacctac aagacggaca ccggacgcgc tgatgtggca tattgtcccc tgattaagtc
    10621 tttatattcc ctgataataa attaccagga taggtgaatc atatcggttt ttcgctgaac
    10681 attttacgtt aacctatctc tgttgctttt ttctccttac ggagaaatac aggtcatccc
    10741 cgcgagggcg tggctgacct cagggggata tcccctgctt acgctggctg gacgccaccg
    10801 acgcacggtc tctccccctc tttctggctc cgtccggctg gacgccggcc acgccagaaa
    10861 ttcacccggc ccccgtcagg ctcacgcctg accacccaca aaaatccacg gtgctggacg
    10921 cacctggcga agcagcaaag ctgctcctga ttttgcgggt ggtcagccgc tcgctatggc
    10981 gaactctgac gggggccgga acaccggaaa agaccgcact ggggtgccgc cttttcctct
    11041 cacacgctgg ggcgtgctcg ttgttcctcc ggggctgcgc cttttccgca tggggcggaa
    11101 agaggctggc ctccgtggtg aaagctgaac gcaatcacca cggacgtgac cgccgccgcc
    11161 atgccttggg gcatgtccgc ggcttgtgca gcgggggctg cacatcacgc cggcaaacca
    11221 tcgtctgaag gagagagaac atgtttaata cggattcgct tgcctggaag tggcacattc
    11281 cggagctggc ccggcagatt tatccgtggg tcagctggct gtaccaggag tattacggtc
    11341 tggccggggc tttcctagtt atcggcctgt ttttcggtct ggctgaactt tgggccggac
    11401 ggatgcgttt tgtggggctg agtcataagg agcgacggga gtatgccgat cgctataagc
    11461 gcgataataa acggccagcg ccattttcgg tgctggcctc gctggattcg cccgggcgga
    11521 tggtcagggc acgtcgacat gtctggaact acgcgtactg gctgaccggc gcggccggtc
    11581 tggcccttct gggggggctg gttgtcgccg tgtttatcga gagctgggga gaaaccgtcc
    11641 tgcgtccgga cctctacgca aacgcagatt attacctgct gcagccggct aacctgttcg
    11701 ggattgcctg gtgcgcgggc gggctggctg tcgggattgt gttggcgcgg ctgctggcgt
    11761 tcctgctgct tgacggtcat tttgcggctg ctgacgaggc ggtgaacgcg aaactgaatc
    11821 aggagagccg gcgcagcagc cagcggacag gagagatgac cgacgtgcgc cacctgcatt
    11881 tcggggagcc ggtaccggtt aatgcgctgg ccgatttttc ctcggagcag gcccggcagg
    11941 agcaggcggt gtttctgggt aaggatgagc agggcaagcc ggtgctggtt ccgcgtgata
    12001 cctggcgcaa gaccaacatt cagatcctcg gcctgcccgg gagcggcaag agcgtgatgg
    12061 ccaccaacgc gctcattcgc tgcgtacgcg atttcgggga cgcggtggtg tacttcgacc
    12121 ccaaagggga cgcatgggcg ccgcacgtct tccgggcgca ctgtccggac ttcaccctgc
    12181 tggatctgcg gccggggaaa ccggcgcagc tgaatctgtt ccgcgatctc gaccagtacg
    12241 cgctgaaaaa cctgctgatt gccgggctta acctgggcga gaccggcgac gtggcggacc
    12301 actaccgcat cagcgagcag aaagcggcga agctgattgc ggagcagttt ccgcaggggg
    12361 caaacattca gcaggtgctg gcggcggcgt acgcgctgcc ggaggccctg aaaaaggacg
    12421 tgaaggggtt gatcactagg ctggaaaacg tcgctgacct gagcgtcctg cagacggaca
    12481 gtggcatcga tgtggcgggg attatcaacg gcggcggctg cctgtacgtt atcggctcga
    12541 tggacgacga ggcggtgatc cgtgtgcaaa aaatgctgtt tgcccgctgc gcgcaaatca
    12601 tcattgcgcg ggacgagttc cgggagtggc cacacgtcag cgtgatgctc gatgaaatta
    12661 aatatctgct gtcgaagtat gtactgaacg cactggggac gctgcgcagc cgggactgca
    12721 atctgttgct cgcgcaccag tcgctgggcg atttcgggca gtgcgggcag gatctgcccg
    12781 ctgaatttgt gaaaaccacg gtactggata acacgccgat ccgctggttt taccgggcgg
    12841 ccagcctgga gtccgcgcag tgggcggcgg ggcagaccgg ggaaatcagg gtggacgtgg
    12901 agcgccgccg tgccagccgg gaggcgggga acgtggagca tatcagcggg gacagcttca
    12961 tccagaagga ggcgcggccg ctgtttgacg tgaacaccct gcaacacctg ccggacgggt
    13021 tcgcggtcat gaccgggctg ggtgtggccc ggatggcgtt cagcagtccg ctgcgggttg
    13081 agcgccggga aatcccgttg cagcgtttcc cggtgctggc gaaaaccgat cccctggcgg
    13141 agttccggcc cccggccgac ggcggcgggc cgaaagcgga tgatgatttt gcggggattt
    13201 actgatggcg ctggagctgt acaacgtcag ccacgcgaaa cgacaggcgc ggaacgcgga
    13261 gaaaacccgg ctgaccctgc gctggttgcg ggaagagctg tgttccacgg cggagctggt
    13321 tgcgcggcgg ctggggattg cggcggtgca gccggtctac cgcttcctcg acagcctggt
    13381 ggcgaaaggg ctgctggtcc ggaccaaata tccggtggac ggccgtcagg tcagcgtgtg
    13441 ggggctgacg ccgcatggcg tggcgttctc gttcgacgag gacgaaccgc tggcggatgt
    13501 tattccgttt cagccgtcaa gggtgtcggc agcacatctg ccgcaccggc tggccgtgca
    13561 atccctgcgc cttgatatgg aagccagagg ggccaccggc tggcgcattc tgcaccgtct
    13621 gagtcttaaa ggcatg
//
