LOCUS       NZ_OZ040966             7323 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30821_1#274 plasmid 4, complete sequence.
ACCESSION   NZ_OZ040966
VERSION     NZ_OZ040966.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068092
            Assembly: GCF_964054525.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964054525.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:55:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,178
            CDSs (total)                      :: 5,063
            Genes (coding)                    :: 4,856
            CDSs (with protein)               :: 4,856
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 83 of 207
            Pseudo Genes (incomplete)         :: 151 of 207
            Pseudo Genes (internal stop)      :: 40 of 207
            Pseudo Genes (multiple problems)  :: 57 of 207
            Pseudo Genes (short protein)      :: 1 of 207
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ040966.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30821_1#274"
                     /db_xref="taxon:562"
                     /plasmid="4"
     gene            16..207
                     /locus_tag="ABGU71_RS25795"
     CDS             16..207
                     /locus_tag="ABGU71_RS25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165978.1"
                     /translation="MTKQEKTALNMARFIRSQTLILLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            877..990
                     /locus_tag="ABGU71_RS25800"
     CDS             877..990
                     /locus_tag="ABGU71_RS25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001428439.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDFTPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(1219..1365)
                     /locus_tag="ABGU71_RS25805"
     CDS             complement(1219..1365)
                     /locus_tag="ABGU71_RS25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071527535.1"
                     /translation="MKKKIGVSIILVLVVLCLTACQNNYVRDVQGGTIAPSSSSKLTGV
                     AIQ"
                     /name="colicin release lysis protein"
     gene            complement(1435..1698)
                     /locus_tag="ABGU71_RS25810"
     CDS             complement(1435..1698)
                     /locus_tag="ABGU71_RS25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038405.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038406.1"
                     /translation="MNKMAIIDLAKSFLASKITAMEFSERICVERRKLYGVKNLSQDVL
                     NCGEELFIAAERFEPDSDRADYEFDDNGLRIEVQSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            complement(1695..3788)
                     /gene="cda"
                     /locus_tag="ABGU71_RS25815"
     CDS             complement(1695..3788)
                     /gene="cda"
                     /locus_tag="ABGU71_RS25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001282379.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRSGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPVSQLPAAGN
                     NVPVKYRLTDLMQNGTQYIAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDRNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVKQPPVY
                     TQEMVERANSVMNEQGTLVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVGPLLMRISLGALWFHSEDAGAGSDIVPGRDMEAMFSLNAQMLAGQGMVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNDSIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPAPQPSAPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDAGGLRDFIYWR
                     PDATGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTNNVVILKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            4046..4483
                     /locus_tag="ABGU71_RS25820"
     CDS             4046..4483
                     /locus_tag="ABGU71_RS25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177063.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTGQDGRR
                     EFETSELIRAYGELKQDETAERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            4567..4986
                     /locus_tag="ABGU71_RS25825"
     CDS             4567..4986
                     /locus_tag="ABGU71_RS25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5471..5704)
                     /locus_tag="ABGU71_RS25830"
     CDS             complement(5471..5704)
                     /locus_tag="ABGU71_RS25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5711..6271)
                     /locus_tag="ABGU71_RS25835"
     CDS             complement(5711..6271)
                     /locus_tag="ABGU71_RS25835"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001428444.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAALKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<6306..6986)
                     /locus_tag="ABGU71_RS25840"
                     /pseudo=""
     CDS             complement(<6306..6986)
                     /locus_tag="ABGU71_RS25840"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6949..7272)
                     /locus_tag="ABGU71_RS25845"
     CDS             complement(6949..7272)
                     /locus_tag="ABGU71_RS25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024177064.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGHWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     BGC             1..7323
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(293..380)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          403..949
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.192"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2..210)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.571"
                     /coverage="100.0"
                     /evalue="7.51e-16"
     Typing          complement(5365..6959)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.308"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggagaaca gaactgtgac taaacaggaa aaaacagccc ttaacatggc ccgctttatc
       61 agaagccaga cgctgatcct gctggaaaaa ctgaatgaac tggacgccga cgaccaggcg
      121 gacatctgcg aagcccttca cgaccacgcc gacgagcttt accgcagctg cctcgcacgc
      181 ttcggggatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgc
      241 ctgtgagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg ttttagcggg
      301 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaatc
      361 gtgtggcatc agtgaggatt gtatgaaaag tgcaccatgt acggtgtgaa atgccgcaca
      421 gatgcgtaag gagaacatgc agatgccgat gctcttccgc ttcctcgctc actgactcgc
      481 tgcgctcggt cgttcggctg cggcgagcgg tgtctgctca cacaaaagcg gcaatactgt
      541 tatccacaga atcaggggat aacgccggaa agaacatgtg agcaacaaga caaagccggg
      601 gaaaaggccg cgccggtggc gctttttcca taggctccgc ccccctgacg agcatcacaa
      661 aaaccgacgc tcaagtcaga ggtggcgaaa cccgacagga cttaaagata ccaggcgttt
      721 ccccccggaa gctccctcgt gcgctctcct gttccgaccc tgccgattac cggatacctg
      781 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg ttggtatctc
      841 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      901 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgacttt
      961 acgccactgg cagcagccat tggtaactga aaagtggatt tagatacgca gaactcttga
     1021 agttgaagcc ttatcgcggc tacactgaaa ggacagcatt tggtatctgt gctccactaa
     1081 agccagttac cttcggaaaa agagttggta gctcttgttc cggcaaacaa accaccgttg
     1141 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1201 aagatccttt aatctttttc actgaatcgc taccccggtt agtttcgagg atgatgatgg
     1261 agcaatcgtt cctccctgaa catcccttac atagttattt tgacaagcag tcagacacaa
     1321 aacaacaaga acgagaataa tcgatacccc tatctttttt ttcataacat ctccttgtca
     1381 tgagtgagta tctgacattt ataattaaat tccagatatt aaaatcactc atatttataa
     1441 tttaaatttt tccaggatag attggacttc aatcctaagt ccattatcat cgaattcata
     1501 atcagcccta tcagaatcag gctcgaaacg ctcagcagct ataaacaact cctccccaca
     1561 attcaataca tcctgagaca aattcttaac accatacaat tttctccgct caacacaaat
     1621 ccgctctgaa aactccattg ctgttatttt tgaagctaaa aatgacttcg ccaaatcgat
     1681 tatcgccatc ttgttcatag ttcacctgtt tctaaataaa ttcgcccatt atccgcatct
     1741 ggatttattt tccacccaga taagaactca ccatttgatt ttagtatcac cacattattt
     1801 gtattaggat taaaataaac ttttgatcct ttttctcgtc ggtatgttcc tttctctact
     1861 gtatccttat cggataaatg ttcctcaata gcatccctga atttagtaag agtttcacga
     1921 ttctttttag tatcactaat accaaaatca ccagcatgtt tatatttttt atcaagctgt
     1981 ttgcgtgaaa accgccctga atccagagga tcattaagca tcacatagac agcttcaaca
     2041 ccagtcccgg tagcatcagg acgccagtaa ataaaatctc gaagtccacc ggcatcagag
     2101 actggagtaa tcgtcgttac cggcatttca accggtttta cctccgtacc tgtatgcact
     2161 ggggtaaccg gaacgggttg atgatttcct gtatctgttg gtgccgaagg ttgtggggcc
     2221 ggatttacca gaatcgtcct ggagggtgca ccgactactg ctggcagcgt aattttatcc
     2281 agtcctgtag ctgtatcacg aacagcatta agaacaggaa ccgcagcagg gatactatca
     2341 ttccctgtct tcagtaaatc cagagcaagt tgtccgttac tgtttatcaa ttgtccacga
     2401 acaggcagat taacactcgt agcaccaggt tcaatgacca tgccctgtcc tgccaacatc
     2461 tgagcattca gcgagaacat agcctccata tctcgccccg gcacgatatc acttcctgct
     2521 ccggcgtctt ctgaatggaa ccacagagcg ccaagagaaa tccgcataag caggggaccc
     2581 acttcgggta tggttacttt ctccagagca ttactgaaaa acttgcttat gttgcctgca
     2641 atatcaccag cagccgtcca gacaccagtt ccggtcatcg ccagttgtac agaactggca
     2701 gtgttattca gaacaagagt tccctgctca ttcattactg aattagccct ttccaccatt
     2761 tcctgtgtat atacaggagg ctgtttcaca cctgctttac taaataactc ggcttttgct
     2821 ttttcagctt cggcccgggc taccgcttct tctttcttga ctctgaaatc atcctttgct
     2881 ttattttcgg cttcctgacg ctgactcaga ccattacggt caagaatctc cacagttgaa
     2941 atatataacg gagaaacacc gctgccgtca ggaaaacgaa caatgacatc atgattatta
     3001 ctggacatcg tataacctgc ctcaacataa ttacctgtct cagcaataac gccctcttcc
     3061 tgaatttcgg gattagacag aaccggagta ttcatctgcc ccacacttaa taccaggtcc
     3121 ggtagatttg gtgatacagg ggcactgtaa acatctttaa tatttgttcc cggacggctc
     3181 cggtccggaa ccggaacggc atcaactacc ggcactgtca tcggaatacc tccgataata
     3241 gcaatatatt gcgtaccgtt ctgcataagg tccgtcagac gatatttcac cggtacatta
     3301 ttacccgctg ctggtaactg gctgacagga atatccgtta cctgctccag ttgctgtgta
     3361 accataagac gaataatttt ttgctcattt ccggaagcag gctccagacg ggcaaaagaa
     3421 acagcagccg ggcgtgatgg ttctggcaaa ccactacgta aaccattctc aagaactgtt
     3481 cgtgcagcag aaactttctc gtttccccag ggactatgca cactgacaga ccatcccgac
     3541 cagtccccct cttcaagttc cttatcaagg ataatccggc gaataattcc tggaaaaaga
     3601 acactttgta caagtgcgat accttgtgga ctgtaatctc cccatcccgg aggcatggat
     3661 ggagcgagac ctgatgaacc accgggtttc acatcaccgc ccggaattcg tccagttgac
     3721 ggccacacaa ccattgtgtc cccgtaatca ataccttctg tcgggacact accttcatta
     3781 tcactcatag aaaacctctt tgacattaaa aacaaatagt taaaagacgc acactgtata
     3841 tacaaccact ggttttatat acagtttaaa attacgccgc tgcgttttcc ctgtcaaccc
     3901 tgtggatttt cgtttttgtg aaaacgatca aaaaaacagc cacccacacc acgtgctatt
     3961 cccacccgat gccacaaaaa ccagcacaaa cattaccgtt ctccggcccc agtatgtttt
     4021 actgaaacca tgatatgaga caccaatggg acactgtcac ttcatggcat ggcacacact
     4081 ccaggacgca ctgaaaatga caggcagatc gcgttcacag ttttaccgtg atatgcgcgc
     4141 aggccttgtc agttaccgta ccggccagga cggacgacgg gagtttgaaa ccagtgaact
     4201 gatccgggca tacggcgaat taaagcagga tgagacagca gaaaggcaca gtgcgggaca
     4261 ggcagaaaat ccacatgatc agcagacaga acgcattctc cgggagctga atgagctgaa
     4321 acaatgcctg acactgatgc ttgaggacaa acaggcacag gatacggaac gccggcgcca
     4381 ggaagccgaa cgggaacagc tacataaaga gatagagcag ctcaggcagg cactggagca
     4441 ggaaaaaaaa cggggattct gggccagact gttcagccgg tgagcatcgg ctgcaagtga
     4501 tgataagata gtcagcggtt aaatataagt cactgagtac aaagatttaa aggtaaggag
     4561 acactcatga gacgttccgg cggcacactg tatcttatcg ccctgctgac agcggcaacg
     4621 gtcctgacag cctgcacgcc aaagggcagt gtggaacaac atacccggca ttacgtttat
     4681 gcatcagacg atggttttga tcctaacttt tccacccaga aggccgacac aacacgaatg
     4741 atggtgcctt ttttccggca gttctgggat atgggagcaa aggataaagc gacaggaaaa
     4801 tcacggagtg atgtgcagca acgcattcag cagtttcaca gccaggaatt tttaaactca
     4861 ctccggggca caactcaatt tgcgggtact gattaccgca gcaaagacct taccccgaaa
     4921 aaatccaggc tgctggctga gacgatttct gcggtttatc tcgatggcta cgagggaaga
     4981 cagtaagtgg atttaccata atcccttaat tgtacgcacc gctgaaatgc gttcagcgcg
     5041 atcacggctg ctgacaggta aaaatggcaa caaaccaccc gaaaagctgc cgcgatcgca
     5101 cctgataaat tttaaccgta tgcatagcta ttcagccatg tgaataacgc tggttttgcc
     5161 tgcgtaaacc tcatgacact gttttttttc catcttttca gttgatgaca tacgcagaca
     5221 tcgcgggatg aggctgagga atgagcgcga tctggcaaag aggcaaaaca cagcaacaaa
     5281 aacgacacgc cagaatcgcg cccggatgca tttttaacgc gttccggtac catctggcaa
     5341 cctcccggaa caactcaccg tcacatcccc attgacgggc cacgccatac ccgtacttcc
     5401 cgttcctgct cttcatgcca ggaccgcgca cgctcccgtt ccaggcgtgc cagcctttcc
     5461 tgttcatccc ttatctgctg ttcgtgataa ataaccgact caagcggtcc acctgcccgg
     5521 ctaatctctg cacctgctga ctcaagtcgt cgcactgttc cctcagttgc ccgttctcct
     5581 gtcgtgtcag ctcgaacata tgctgcaaat ccgtgaaggc gctctcccag tctttcagcc
     5641 gctgcgtata gtcctgctgc aattgctcta aggcgttcag taagtgcatt tccagctctg
     5701 tcatactcac ttactccctg accagtctta ctgcgttctt cttctccacc gtccagttgt
     5761 tttccccctt caccccggac ggcaatacca gaaatttccc gttcctgccc tcgtgatacg
     5821 tcacacccca tgttttttcc cggagtttcg ccagcgtctc ttcctggtcc ctgatagcca
     5881 ggatgttcgc cgcaatccgg ctttcctgcc actgaatcag cccccatgac gccagaaaaa
     5941 tcccgcccgt gacgcaaagc gccgtcagcg acgggtacag tatccgccct ttgatcagct
     6001 tccagagcag ctcttcctgc cgccgggcca gttcgttctc tgtggcgctg aactgcgcgt
     6061 tcacggcact gttcagcgtc tccagttgtt ctttcagcgc tgctgtgtgt gcgctgatag
     6121 cgtctctgat tttctgcccg tttaagttca gttccctgtc tacagacgct tcgagcttcc
     6181 tgaactcgct gttcagcatg ttctctgtag agacggcacg ctctttcagt ttcttctcga
     6241 agtctgtccc catttgtaaa agattgctca tacagcgccc ctttcagcct gagattacgc
     6301 ccgccctccg ggtcggcgat actgatactg ctcctggttg tcctcaccac ctcaaaacct
     6361 gccgctgtaa gcgcctcagt gacatcctga cgcgttttta gcgctccggc atgataaaga
     6421 gcctccagcc ccctcgtaat cgcttctgcg gcctcctgtt tcgctttcgg cagattattc
     6481 ggggtgacaa gtgtccgcct gttctccggt gcgttcgggt cgtgcagccc gtaatgatga
     6541 ttcaccagtg tctgccaggc attgattcgc ggacggtccg cacggtcgta atagggctgg
     6601 agccgttttc cgctcaccag ctccatattc gggatgacaa aattcagctc aagacgcccc
     6661 ttgtcctggt gctccaccca caggatgctg tactgatttt tttcaagacc gggcatcagt
     6721 acccgctcaa agctctccat caccctttca cgctctcccg gtggcagtgt ctgctctgca
     6781 aaagacagaa cccccgaggt gtattttttc gcaaacggcg tggcatcgat gagttcccgc
     6841 acctcttcgg gatcaccccg cagaattctc gccccttccc ggttacgctc ccggcccagc
     6901 aggtaatcaa ccggaccact gccaccgcct tttcccctgg catgaaactt aactatcatc
     6961 ccgttctccc tgtttacgga cctcatccct cagctcactc agttcacgtc cgatggccat
     7021 cagtgcagcc accacatgaa cccggtcatg ccccgaccag tgtccgctgt ttatcttccg
     7081 ggctatctga ttcaggttat tgccgaccga agcgaactgg cgcaacagcg gcggtgccag
     7141 tgtcggaaga cctgacgttt tcgatggcgg tgcacccagg cagaccttac gcatccatga
     7201 cgcaagttgt tttccctcac aacgtgccag cagccgcgca tgttcctcat ccgtgacccg
     7261 tatcgtgagc atcctttcgc gtttcaccgg tatcattaaa aacctccgac agactcccca
     7321 cac
//
