LOCUS       NZ_OZ040965             8554 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30821_1#274 plasmid 3, complete sequence.
ACCESSION   NZ_OZ040965
VERSION     NZ_OZ040965.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068092
            Assembly: GCF_964054525.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964054525.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:55:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,178
            CDSs (total)                      :: 5,063
            Genes (coding)                    :: 4,856
            CDSs (with protein)               :: 4,856
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 83 of 207
            Pseudo Genes (incomplete)         :: 151 of 207
            Pseudo Genes (internal stop)      :: 40 of 207
            Pseudo Genes (multiple problems)  :: 57 of 207
            Pseudo Genes (short protein)      :: 1 of 207
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ040965.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30821_1#274"
                     /db_xref="taxon:562"
                     /plasmid="3"
     gene            join(8522..8554,1..1857)
                     /gene="cya"
                     /locus_tag="ABGU71_RS25735"
     CDS             join(8522..8554,1..1857)
                     /gene="cya"
                     /locus_tag="ABGU71_RS25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin Y"
                     /protein_id="WP_113705710.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGITSTQAKQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLLPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKDMGRVTERVVDAVNLALILNQIS
                     SGMSASQKDVDAANRIINETVKAINDVNLKIAEKKKQQVPLLSLMKQKQKEVEELKKVF
                     KNHSYHRIRDAQRAYDDARNKNDLLVSDINALQAQVSGLTARKQQAEKNKAAAEKAKAD
                     AKAKAEAEKAAAEAKAKAEAEKARKEAEAKENNEKAVLTKASEIIISVGDKVGEYLGDK
                     YKALSREIAGNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDVIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMREVESWVVSGIASAVAL
                     AIFSATLGAYLLAVGASAAVVGIIGIIIASFIGALIDDKFIDRLNNEIIRPAY"
                     /name="cya"
     gene            complement(1885..2409)
                     /gene="cui"
                     /locus_tag="ABGU71_RS25740"
     CDS             complement(1885..2409)
                     /gene="cui"
                     /locus_tag="ABGU71_RS25740"
                     /inference="COORDINATES: protein motif:HMM:NF015795.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="WP_021534853.1"
                     /translation="MRKEHTDNKDLNRTLFLITFAGITPLIIIFITYTSNPKFYLISII
                     FDNTQNIPSIISAYNPVMTKVMDIYGKSAPLLALIAFTLQLRDRKLETIANREKLITAS
                     IFSPFFYAFYAYFFLWNNFELTTAGRTVRWMSDNDFTLFIFYACLYFCSFFMTYALCYI
                     PVVSYKLWKER"
                     /name="cui"
     gene            2529..2669
                     /gene="crl"
                     /locus_tag="ABGU71_RS25745"
     CDS             2529..2669
                     /gene="crl"
                     /locus_tag="ABGU71_RS25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WP_010892547.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSKLTGIAV
                     Q"
                     /name="crl"
     gene            complement(2866..3024)
                     /locus_tag="ABGU71_RS25750"
     CDS             complement(2866..3024)
                     /locus_tag="ABGU71_RS25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            3324..3542
                     /locus_tag="ABGU71_RS25755"
     CDS             3324..3542
                     /locus_tag="ABGU71_RS25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072021258.1"
                     /translation="MFFPALSPDSVDNRITAFECAGTARRSRTTERSESVSEEAEECRK
                     LHFLLTHLCGISHRLWCTFHTIRTDAA"
                     /name="hypothetical protein"
     gene            complement(3694..3885)
                     /locus_tag="ABGU71_RS25760"
     CDS             complement(3694..3885)
                     /locus_tag="ABGU71_RS25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042634456.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3950..4273
                     /gene="mobC"
                     /locus_tag="ABGU71_RS25765"
     CDS             3950..4273
                     /gene="mobC"
                     /locus_tag="ABGU71_RS25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_032286519.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQMAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="mobC"
     gene            4482..>4913
                     /locus_tag="ABGU71_RS25770"
                     /pseudo=""
     CDS             4482..>4913
                     /locus_tag="ABGU71_RS25770"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4951..5481
                     /locus_tag="ABGU71_RS25775"
     CDS             4951..5481
                     /locus_tag="ABGU71_RS25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_058813529.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTISTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTE
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            5488..5721
                     /locus_tag="ABGU71_RS25780"
     CDS             5488..5721
                     /locus_tag="ABGU71_RS25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021534848.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(7350..7778)
                     /locus_tag="ABGU71_RS25785"
     CDS             complement(7350..7778)
                     /locus_tag="ABGU71_RS25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021536732.1"
                     /translation="MKRSGGTRYLYLISLITVAAALTACTPKGSVEQYTRHYVYASDDR
                     SDPNFYTNKADTTRMMIPFFQQFREMGEKDKAAGVSAETAQQRIKEFHSEKFLQSLRST
                     TTFAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(7859..8296)
                     /locus_tag="ABGU71_RS25790"
     CDS             complement(7859..8296)
                     /locus_tag="ABGU71_RS25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536733.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQESEREQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     Typing          3522..3609
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          complement(2953..3496)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.689"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4264..5804
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.898"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgccggggt ttaattacgg tgggtatggt gacggtactg gctggagttc tgaaagtggt
       61 ggtccagctc caggcggtgg tatgcatgga aatagtggag gccagcgcgg agataacgca
      121 aacagtagca attctgtgag tcagcagatt tctgccattc agaatgatca gaaacttaaa
      181 cagaaagtgg tgaatatgct gattgctgcg agaaaaatga atcctgaagc aaaaatgatt
      241 ctgggaagta ttgctccttc tggtgttatg caggtcacca ttgaggggat aacctccact
      301 caggccaagc agcttggttt gggagggctg gtcatgggat ataacgcttc tggtgttatt
      361 ggggctgtgg gcgaaataga caccggtcac cgccttaatg catcaggagc aagtactccc
      421 ggaagcgaaa cttctgtgga gagttttgtg aacggacaga aacccgccgg agaatggcat
      481 gctgtggcga aggacagctg gacaggggct ggtccagtga atgtagggct ggttaataac
      541 gccatcaaaa gtgtccggat tatcaaaaaa ggctatgtga ctggtgttct cttgcctgaa
      601 gaggtgatga ataaagcaga gtacaaagca atgcggcagg ctttcgattc ccttccactg
      661 gcaaaacagg gagaggctgt cagacagatt gttgcagcct ggagtctggc atatcaggat
      721 ttcccggtaa atctgaaaaa agatatgggg cgagttactg aacgggtggt ggatgcggtt
      781 aatttggcgc tcattctcaa ccagatatca tccggaatgt cggcctcaca gaaggatgtt
      841 gatgcggcga accggattat taatgagacc gtgaaagcca ttaacgacgt aaatcttaaa
      901 atagcggaga agaagaaaca acaggtacca ttattaagcc ttatgaaaca aaaacagaaa
      961 gaggttgaag aactaaaaaa agtatttaag aaccattctt atcaccgtat tcgtgatgct
     1021 cagagagcat atgatgatgc ccggaacaaa aatgatttac tggtcagtga tattaatgcg
     1081 ctgcaggcac aggtttccgg actgactgca aggaaacagc aggcagaaaa gaacaaggcg
     1141 gcagcagaaa aagcaaaagc tgatgcaaag gcgaaagctg aagctgaaaa agcggctgct
     1201 gaagcaaaag caaaagctga agctgaaaaa gccagaaagg aagctgaagc aaaagaaaat
     1261 aacgagaaag ctgttctgac aaaagccagt gaaattatca ttagtgtggg tgataaggtc
     1321 ggagaatatc ttggcgataa atataaagct ctttctcgtg agatagcagg taatatcaaa
     1381 aactttcaag gtaagacgat ccgtagttat gatgaggcga tggcttctgt caataaactg
     1441 atggctaatc ctgatcttaa aattaatgct gcagacaggg atgtcattgt gaatgcctgg
     1501 aaagcatttg atgcagagga tatggggaat aagtttgccg cgctgggtaa gacatttaaa
     1561 gccgcagatt atgtgatgaa ggcaaataac gtccgggaga agagtataga gggatatcag
     1621 accggaaact gggggccgtt aatgcgggag gtcgagtcct gggttgtcag tggtattgct
     1681 tctgctgtgg ctcttgctat tttttctgcc acccttggag cttacctgct tgctgtgggg
     1741 gcatcagctg cagtcgtagg gattatcggt attattatag ccagttttat tggagcattg
     1801 attgatgaca aatttataga caggttaaat aacgaaatta ttcgccctgc gtattaacct
     1861 agagtcgaaa ggtagtcaag aagactacct ttctttccag agtttatatg acacaaccgg
     1921 aatataacac agagcataag tcataaagaa agagcaaaaa tagaggcagg cgtaaaaaat
     1981 aaacaaagta aaatcattat cggacatcca tctgacagtc ctacctgcag tggttaattc
     2041 gaaattattc cacagaaaga aatatgcata aaaagcataa aaaaatggac taaatatgct
     2101 ggcagtaatt agtttttctc tgttggctat tgtctctagc tttctgtcgc ggagttggag
     2161 agtaaaagca ataagtgcca gtaagggagc gcttttcccg tagatatcca tcacctttgt
     2221 catcacagga ttgtatgcgg aaattattga aggtatattt tgtgtgttat caaaaatgat
     2281 gctaattaaa tagaacttgg ggtttgatgt ataagttata aaaataatga ttaatggtgt
     2341 tatgcctgca aaagtaatca aaaaaagcgt cctgtttaaa tctttgttgt cagtgtgctc
     2401 ttttctcatt ggtataactc tccaagcttt tcattattat taatttagtt gtagatgtgt
     2461 ataagcatat catgctgtac aagtaatggt catcctaaga ttaggggtat gtgttaatga
     2521 gggaatatat gaaaatgata ttatcaggag ggatgcttgt cttgatggct ttgctatcag
     2581 gctgccaagc taattacatc cgcgatgttc agggagggac tgtcgcacca tcctcctctt
     2641 ctaaactgac ggggatcgcg gttcagtaga aaagttaaaa ggatcttctt gagatccttt
     2701 ttttctgcgc gtaatctgct gcctgtaaac gaaaaaaccg cctggggagg cggtttgatc
     2761 gaaggttagg tcagttgggg aactgcttaa ccaaggtaac tggcttcagc agagcgcaga
     2821 taccaaatac tgtcctttca gtgtagccgt agtcgggcca ctacttcaag aactctgtag
     2881 caccgtttgt gccatcatcg ctctgctaat ccggttacca gtggctgctg ccagtggcgt
     2941 taaggcgtgc cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3001 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccaagcc
     3061 gagataccaa cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3121 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3181 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3241 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcggcg cggcctttcg
     3301 ttggcctttg cctttttgct cacatgttct ttccggcctt atcccctgat tctgtggata
     3361 accgtattac cgcctttgag tgcgccggta ccgctcgccg cagccgaacg accgagcgta
     3421 gcgagtcagt gagcgaggaa gcggaagagt gtcggaagtt gcattttctc cttacgcatc
     3481 tgtgcggcat ttcacatcgg ctatggtgca cttttcatac aatccgcact gatgccgcat
     3541 agttaagcca gtatacactc cgctatcgct atgtgactgg gtcagggctg cgccccgaca
     3601 cccgctaaaa cctgctgacg cgccctgacg ggcttgtctg ctctcggcat ccattcacag
     3661 accagttgtg tacgtctccg ggagctgcat gtgtcagtgg ttttcaccgt catccccgaa
     3721 gcgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg tgaagcgatt cacagatatc
     3781 tgcctgttca tccgcgtcca gttcgttgag cttctccagc agcgttaatg tctggcttct
     3841 gataaagcgg gccatgttaa gggcggtttt ttcctgtttt gtcactgatg cctccgtgta
     3901 agggggattt ctgttcatgg gggtaatgat accgatgaaa cgcgagagga tgctcacgat
     3961 acgggttact gatgatgaac atgcccggtt actggaacgc tgtgagggta aacaaatggc
     4021 ggtatggatg cggcgggtct gcctgggtga gccggttgcc cgttccggga agctgccgac
     4081 actggcaccg ccgttactgc gtcagctggc cgccatcggg aataacctga atcagacagc
     4141 gaggaaggtg aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg tggccgcact
     4201 gatggccatc ggggatgagc tgcgccggct gcgtctggcc gtcagggaac aggggacgcg
     4261 ggatgatagt taagtttcat gccaggggaa aaagtggtgg cagtggtccg gttgattacc
     4321 tgctgggaag ggagcgtaac cgggaagggg caacggtgct tcagggtaat ccggaagaag
     4381 tccgggaact catcgatgcc acgccatttg cgaagaaata cacgtcaggt gttctgtcgt
     4441 tcgcggagaa ggagctgccg ccgggaggac gtgaaaaagt gatggcgagc tttgagcgtg
     4501 tactgatgcc cggtctcgaa aaaaatcagt acagcatcct gtgggtggag caccaggaca
     4561 agggacggct tgagctgaat tttgtcattc cgaacatgga gctacagagc ggaaaacgcc
     4621 tccagccgta ctacgaccgg gcagacaggc cgagaattga tgcctggcag acgctggtta
     4681 atcaccatta cgggctgcat gaccccaacg ccccggagaa ccgccggata ctgactctcc
     4741 ctgataacct gccggaaaag aaacaggcgc ttgcagagag cgtcacacgc gggatagacg
     4801 ctctttatca tgtcggggag ataaaaggcc gtcaggatgt gattcaggcg ctcacggagg
     4861 cagggattga agtggtcagg gtgacgcgaa gcagtatcag cattgcagac ccgaacggag
     4921 ggaagaatat cagactgaaa ggagcgtttt atgagcaatc ttttacagac gggcgcggag
     4981 ttcgagaaaa agctgaaaga gagagccgaa tctacagaga aaatgctgaa cgacgagttc
     5041 aggaagctcg aagaatctgt aaacagggaa ctgacctcaa acgaatcttt aatcaggaac
     5101 gctatcaacg accatacaac agccctgaaa gaactgctgg agagatatca gaaaacgacc
     5161 atcagcacga tggatacaca ctggcaaacc gtactgaaaa tgagcgtgaa acgctggctg
     5221 tggctgatta tcgtctcagt gctgatgttt gccacgaccg ggagtctgct gtggtatcag
     5281 ggcatgaaga taaatgcaaa catgaatatt ctccgcgagc agaaagagag tctggagaag
     5341 ctgaacgcga agacatgggg cgtgcgttat cacgaggaca gcaacgggcg ttttcttgtg
     5401 ttgccgaagg ggatgaaagc cgaaacgaac tggacggagg acaacggcaa actgaatgca
     5461 gtgagactgg tacaggagtg acagagcatg acggagctgg aaaaacattt gctgaacgca
     5521 ttagagcaac tgcaacagga ctatatgcag cggctgaacg aatgggagag cgccttcgtg
     5581 gaattgcaga aaatgttttc gcttacgcaa cgggacaacg cgatgctgaa cgagcgggtc
     5641 atgaagttga gtcagcaggt gcagcacttg agcgagcgga cagagcgctt gagccagtta
     5701 tacagcgaga acaggagata agggacgaac ggctgatgca ggaacgcgaa catgagttat
     5761 ctctggaacg ggagcggaag ctggatggcc cttcgctggg atggtgactg aaagtccggt
     5821 tgagtcgtaa acagaatatt ttccagaaat gtcaggctgc ctattggtaa tataaccagt
     5881 cgttcttgcg gtttattgat ttttgtacag gttgttgctg ttttttttgt aaagaggaca
     5941 ggtatttttc tgaaaatgaa cttgcgggaa tgcgcgggtt atgtaaaaaa tataattgcg
     6001 tatctccgat atgtttgtaa taccgatgtg gacacagcga atctggaagg ggtcggagat
     6061 gcgcgttctc tcatgtgtta atattgagtt cttacatgtg gttgcatatt ttttttgcat
     6121 atttgaagtc tgcgaattat attctgatat gcaaaagtca ggactagaca tactatccat
     6181 cgattcagtc cttaccttac gccctcactg atttcctgcc gtgagggctt ttttcatggg
     6241 tgtgaaaaaa ggaagcccca cctgacggag gggctgagtg atacagagca aaaaaatatc
     6301 ctggtgttgg tcacctctgg agagtaataa gattaaccat aaaacaaact ataaacaaga
     6361 atattgatgc caggaatttt cctggttatt ttttttgttt tttacaaaaa agacaaaata
     6421 acagaaagta aaatctgatc atttttgttg attgttattg ctctgtaccg ttattcttaa
     6481 gctgaaagtg aattcttcta attttttctt atattcagac gcaattaaca gtccctgctc
     6541 cttgagggac ttttttttgt ctttttctct ctgttttata ttgttttgag tagtaactat
     6601 aaaggcagtg aagttctgtc ttatgagcgc gtttctggcg gtgatgcgac taacccattc
     6661 tacatcaacg aacaattcac cagaaacgcg cctgtaaatt tttagctgag caagggctct
     6721 gtgtcgagag ttgtggatgt ctggttcgcc attttaaaaa acttcttccg gcggttttta
     6781 gtttcgatgc aggtcagggg gcgctgtaaa gttatgttcc ctgattactg tgccagaatc
     6841 ctgagacaga caggaggtct accggttatt tttcgaattg ggcgcttccg gaagagagag
     6901 tttcattggg cgttggtcgc ggatggtgag cacgtttcga tgttgtgtga cgaacattca
     6961 gctcagttga tggagactgt cagaaacgcg ctcagaagtg ttgtaacgcg atttctggta
     7021 tcaggcaatt gtttcctttt tctggagcat gatacctgtg tgtacggtga tgagttctgt
     7081 cacgtacagt aatatttccc tcctgatgct tccatctggc aggagttgaa cctgccgaaa
     7141 atatgaaaaa acccccgttt cagttacatc aggcaggcaa ttcgttcgtg attcgcatgg
     7201 ctgcatagat atgcatgagg ttaaaattta tcaggtgcga tcgcggcggt ttttcgggtg
     7261 gtttgttgtc atttttacct gtctgctgcc gtgatcgcgc tgaacgcatt tcagtggtgc
     7321 gtacaattaa gggattatgg taaatccatt tactgtcttc cctcgtagcc atcgagataa
     7381 accgcagaaa tcgtgtcagc cagtagcttc attttttccg gtgatggcat atcactgttc
     7441 gtatattttc tgcccgcaaa ggtggttgtg cttctgagag actgaagaaa tttttctgag
     7501 tggaattctt ttatgcgttg ctgtgccgtt tctgctgata ctccggctgc tttgtctttc
     7561 tcccccatct ccctgaactg ctgaaagaac ggaatcatca tccgtgtggt gtcggccttg
     7621 ttggtgtaga agttagggtc agaacggtca tcggaagcgt acacataatg tcgggtatat
     7681 tgttccacac tgcctttcgg ggtacaggcc gtgagggctg cagccacagt tatcagggat
     7741 ataagataca gatagcgtgt gccaccagaa cgtttcatac gtgtctcctt accttaaagt
     7801 atttattctc ggtgagttat tatccgaata ctatcttatc atcagcctct gaccgagttc
     7861 agtgaccgaa gagtctggac cagaatcccc gtttcttttc ctgttccagt gcctgcctga
     7921 gctgggctat ctcattctgt aactgttccc gttctgattc ctggtgcctg cgatccatat
     7981 cctgtgcctg tttatcctca agcatcagcg tcaggcattg tttcagctca ttcagttccc
     8041 ggagaatgcg ttctgtctgc tgatcatgtg gattttctgc ctgtcccaca gtgtgccttt
     8101 cttgtgtctc attttgcttt aattcgccgt atgcccggat cagttcactg gtttcaaact
     8161 cccttcgtcc gtcctggctg gtacggtaac tgacaagacc tgagcgcata tcacggtaaa
     8221 actgtgaacg cgatcttcca gtcattttca gtgcgtcccg gagtgtgtgc catgccataa
     8281 agttacagtg tcccataggt gtctcatctc atggtttcag taaaacataa tgaggtctga
     8341 gaacggtaat gtttgcgccg gtttttgtgg tatccggcgg gaatagcgcg tggtgtgaga
     8401 gactgttttt ttgatcgttt tcacaaaaat agaagtccac agtcttgaca gggaaaatgc
     8461 agcggcgtag cttttgtgct gtacataaaa ccagtggtta tgtatacagt atttgttttt
     8521 aatggtttgt tttaaaagtc aaagaggaat gaat
//
