LOCUS       NZ_OZ040953             4069 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30821_1#330 plasmid 4, complete sequence.
ACCESSION   NZ_OZ040953
VERSION     NZ_OZ040953.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068043
            Assembly: GCF_964054465.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964054465.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:56:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,082
            CDSs (total)                      :: 4,968
            Genes (coding)                    :: 4,761
            CDSs (with protein)               :: 4,761
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 79 of 207
            Pseudo Genes (incomplete)         :: 150 of 207
            Pseudo Genes (internal stop)      :: 39 of 207
            Pseudo Genes (multiple problems)  :: 51 of 207
            Pseudo Genes (short protein)      :: 1 of 207
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ040953.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30821_1#330"
                     /db_xref="taxon:562"
                     /plasmid="4"
     gene            1..2160
                     /gene="mobQ"
                     /locus_tag="ABGU72_RS25395"
     CDS             1..2160
                     /gene="mobQ"
                     /locus_tag="ABGU72_RS25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_029976510.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAEHRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2398..3105)
                     /locus_tag="ABGU72_RS25400"
     CDS             complement(2398..3105)
                     /locus_tag="ABGU72_RS25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_024623342.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIQPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3637..3891)
                     /gene="mobC"
                     /locus_tag="ABGU72_RS25405"
     CDS             complement(3637..3891)
                     /gene="mobC"
                     /locus_tag="ABGU72_RS25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          2..359
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.443"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3893..4069)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          complement(2399..3105)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.859"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..2157
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.861"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcgattt atcatctcag catgaaaatc atctccagaa acagcggcta cagcgccgtt
       61 gcttctgctg cctatcgttc cggttcactg atgcttgatg agcgtaccgg actgactcac
      121 gactacaccc gcaaaagcgg tgtggctgaa gctgtgattc tgactcccgc gacagcccct
      181 gcctggtgta cgaaccgtgc cgaactctgg aatgccgttg agaaggccga acaccggaag
      241 aactcacaac ttgcgcggga aattgaactc gccatccccc gcgaactgcc gcaggatgcc
      301 gcacgggaaa ccgttctcgc ctttgtcaga gaaaactttg tcagtcaggg catgattgct
      361 gatgtggcgt tccatcacat ggataaaaca aatccccatg cgcacatcat gctgaccacg
      421 agagctgtcg gacctgctgg attcggtgga aaggtcaggg actggaacga ccggacacat
      481 gccgagacgt ggcgtgcatc atgggctgac catgcgaaca gggcactggc gaacgccggt
      541 tatcaggaag agatagacca tcgttcatac gagcgacagg ggcttgagaa aacgcctggt
      601 attcacctcg gaaagtctgc ctgtgcgatg gaaacacgcg gtatcgaaac agaacgcggc
      661 gaacagaatc gtctgattaa cagacttaac ctggaaatac agatttcccg taccgaactc
      721 agaaacagcg ggctgacaga gcctgcccgc cgtgtggctg accttctgaa tatcgtgctt
      781 cctgacaacg caacttcgta cacactacgt gacatcatcg aagcgttgcc gaaggacagt
      841 aatgcggcat ggaaactcac cagtaatgcc atgtcgatga tggcagatat gcaggcgacg
      901 cgcagacgct gggaagagct gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc
      961 ctgaaaaaat catcgccgtt ttcgtccggt ttttcccgta ttcccctgat gcaatgggcg
     1021 gcaccggaat accgtagcga acagggaaaa atccagtcgc tgagccagca aatggaaaaa
     1081 ttgcgacagc attaccggaa ggtggagaaa caggacattc ccgcgagtca gacggcattt
     1141 gaaaaacagt ggcagcagtg gggggcatcc ggccttgcag ctctcagagt ccagttgctt
     1201 aaacgtgaag aggaattgcg ccggaaggag caggaggata cagaacgtag tcgcgcggag
     1261 cgacgagcgg aacagctcag gaaaaatgac aatgccgtta tggattcagg ggtactgagc
     1321 gcagtcgtta cggggtatgg cgaggccccc atgcctggta acggggaaat gacctgttac
     1381 cttctgcttc acaaccggaa cggagaatac acgctctggg ggaacgagct ggagaaatac
     1441 cgcaccagaa tctttgagcg tgttgattta atgcgtgaca ggagtggtta tatctgtgac
     1501 cgaagcgaaa tcggtcaaca tccaccactg caacgtgttt attcctctgc gacctttgag
     1561 caacttctga cgcaggtttg ccagacatgg ccgcaatata cccgcaatct ccgccagccg
     1621 aagacatggc cggagtcttt ctgtcttgga gaagaccgcc agccagccat gccatcactg
     1681 gcagcaagga aggtggattt tacgcaggga cgtctgctac cgacgctgat gccggtaatg
     1741 agttctgtcg accgggaaac ccgccagctt cagcttttgc tggtcatggg cgtggatgac
     1801 tctctgggcg gtgttgtccg gcttaatggt acgctgtatc ccgcgtttgc cgttccctca
     1861 gccgataaca gccagctggt catcagtgca ctgaccgata aggggttacg gtatgcaggg
     1921 tatggtgttg ccgtaaatca cgatgcagac agccacatat cacctgcgcc ggaacttatg
     1981 gagtttcatc tgaaaacccg tgaggcaccg ctttttgctg ccgtcaacac accagaaaaa
     2041 cagccggatc atctgttccg ctcgctgggt tttaatcgta cctgggacga atggcggcgc
     2101 gaggaagatg cacgaacaca cgccaccgag aggagacatg atcgcggttg gtcacagtga
     2161 atgtgaacaa tattttgttt tgtttaacat cgtgttatgt tcgtcgatgt tcatcgacag
     2221 ggatgaatgc aagaagcaca cacagcatag ttttgttttt ccgcccccaa aaattggggg
     2281 ttcttttttc gctgcattag agcgcttttg acgcaatttt tcacttcaat agtaccttcg
     2341 cactactaac acaaaaaaaa cgcagcaggg agcgttttag gtgccttaaa atcgattcta
     2401 gatcgtcatc ttgatctgtt tgtcttcctt gaagcgaaaa tgcagggcta caaccgtgcg
     2461 cccctttttg atcgtctcaa ccgttacagc caggtcgctt tttttgttca gttcgtcaac
     2521 acagggttgt attagatccc tgtttaacgt tttgaaatct ttatatttac catcaagcat
     2581 cagtgatgat ttaaaatcat cgagggcgat aatacgttcc ccagtactgc ggaattgctg
     2641 gagaatttcg tatattctta tgctgtaact tcttgataaa ttagaaatat ttttaatgac
     2701 gactctggtg aattgcccct tcaactgtgt cagataaggc attaccgcgt cagaaaaagt
     2761 gatttgtgct ttcccctctc ccctcgcgta ctgtgcccga tactgaatcc agcggaactc
     2821 ctcgcgcttc tcgtcgtcct tgagaatgat cgacctgtcc cacaaacgat cgatcgcttc
     2881 ctgtagctga acctcggcat ttttccgccc catatcaggg aatgaatcaa aatattccgc
     2941 agctgtaatg gtcattgtct tttgcaattc aggtgaagct acgtcagtac cggagttaag
     3001 tcggcttatg cagagcaata agaaacgctg ttcttgtaaa gttagcttgt aactggcctc
     3061 aaccagcgca ttggacttat aggctatcag agggtttgat gtcattttaa ccaccaggcc
     3121 aaaaatgagg tgaaataaaa acctatcacc tttagcattg gtgagcaatt atattttcac
     3181 cccccatttt cctgtgaata ttaccttaaa tctcctcacc tttaccttaa attacctcac
     3241 ccaagcacct taaattatat cacctttacc ttaaatcata tcacctatag cgttgtaaca
     3301 ttttgttttc tataaggaat tttggcgcta aaacaagtat taaaaacaag atcttaaaaa
     3361 caagatctaa aaacttaacg acgagcctgt ggataacttg gagtgctgaa aacaaaaaac
     3421 caataaaaac aatgcgttgc aaaaaagcag aattgcaagt tccgaataca ccccctccaa
     3481 ccctgtggaa atccagtatt catgcgggtt cagcgcatat cacctcccga aaaaccgtgc
     3541 gtatgtgctc aatcaggcga cggattcact tccgcttcct cgcccactcg ctcgctaacg
     3601 ctcggtcgct gggactgcgg cgagcggacg atacaatcac tgtcctggca gcgcttccag
     3661 ctcgttaatc agcggcagta tgcgcgtcac atcagcctgt cgtgtcacct ctgccttcag
     3721 gaactggagt aaccaggcac gcaactgcac ctcctccggc tttctgacct ttgccagcaa
     3781 tgctgcaccc acaatgacct tctgcgctgt gtccagactc ctgctcttcg ccttcaggcg
     3841 ctgtaatctg gcctcagcat cagcaatctt ctgttcgagt gttctgctca tgatgtaact
     3901 ccgtgcgcga tgaaaaatcg cattttagcg cgtcactggt agtttaaaaa ctgagctggc
     3961 ataatgcacg gaaaatcacg aagtgcgcac ttatacaatc tccactccgt ttcgattgtg
     4021 tgcgccctgc ggggctgaaa gaaaaccaca aaaaggcatt acggcagaa
//
