LOCUS       NZ_OZ040767             7942 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30224_1#38 plasmid 4, complete sequence.
ACCESSION   NZ_OZ040767
VERSION     NZ_OZ040767.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068048
            Assembly: GCF_964053165.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964053165.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:51:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,951
            CDSs (total)                      :: 4,839
            Genes (coding)                    :: 4,635
            CDSs (with protein)               :: 4,635
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 78 of 204
            Pseudo Genes (incomplete)         :: 148 of 204
            Pseudo Genes (internal stop)      :: 41 of 204
            Pseudo Genes (multiple problems)  :: 54 of 204
            Pseudo Genes (short protein)      :: 1 of 204
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ040767.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30224_1#38"
                     /db_xref="taxon:562"
                     /plasmid="4"
     gene            1..804
                     /gene="aph(3'')-Ib"
                     /locus_tag="ABGU60_RS24710"
     ARG             2..801
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            804..1640
                     /locus_tag="ABGU60_RS24715"
     CDS             804..1640
                     /locus_tag="ABGU60_RS24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(1701..2405)
                     /locus_tag="ABGU60_RS24720"
     CDS             complement(1701..2405)
                     /locus_tag="ABGU60_RS24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <2469..2807
                     /locus_tag="ABGU60_RS24725"
                     /pseudo=""
     CDS             <2469..2807
                     /locus_tag="ABGU60_RS24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            2990..3850
                     /locus_tag="ABGU60_RS24730"
     CDS             2990..3850
                     /locus_tag="ABGU60_RS24730"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            <3997..>4278
                     /locus_tag="ABGU60_RS24735"
                     /pseudo=""
     CDS             <3997..>4278
                     /locus_tag="ABGU60_RS24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /name="transposase zinc-binding domain-containing protein"
     gene            4448..5152
                     /locus_tag="ABGU60_RS24740"
     CDS             4448..5152
                     /locus_tag="ABGU60_RS24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <5202..5921
                     /locus_tag="ABGU60_RS24745"
                     /pseudo=""
     CDS             <5202..5921
                     /locus_tag="ABGU60_RS24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /name="helicase RepA family protein"
     gene            5908..6759
                     /gene="repC"
                     /locus_tag="ABGU60_RS24750"
     CDS             5908..6759
                     /gene="repC"
                     /locus_tag="ABGU60_RS24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            7067..7882
                     /gene="sul2"
                     /locus_tag="ABGU60_RS24755"
     ARG             7068..7879
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             805..1637
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2991..3847
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(5044..5927)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgaatcgaa ctaatatttt ttttggtgaa tcgcattctg actggttgcc tgtcagaggc
       61 ggagaatctg gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca
      121 cctgcttccc gccgcggtga gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt
      181 cgaggtgtgg cttgccccga ggtcatcaac tggcaggagg aacaggaggg tgcatgcttg
      241 gtgataacgg caattccggg agtaccggcg gctgatctgt ctggagcgga tttgctcaaa
      301 gcgtggccgt caatggggca gcaacttggc gctgttcaca gcctatcggt tgatcaatgt
      361 ccgtttgagc gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat
      421 gccgtcaatc ccgacttctt accggacgag gacaagagta cgccgcagct cgatcttttg
      481 gctcgtgtcg aacgagagct accggtgcgg ctcgaccaag agcgcaccga tatggttgtt
      541 tgccatggtg atccctgcat gccgaacttc atggtggacc ctaaaactct tcaatgcacg
      601 ggtctgatcg accttgggcg gctcggaaca gcagatcgct atgccgattt ggcactcatg
      661 attgctaacg ccgaagagaa ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc
      721 ctattcaatg tattggggat cgaagccccc gaccgcgaac gccttgcctt ctatctgcga
      781 ttggaccctc tgacttgggg ttgatgttca tgccgcctgt ttttcctgct cattggcacg
      841 tttcgcaacc tgttctcatt gcggacacct tttccagcct cgtttggaaa gtttcattgc
      901 cagacgggac tcctgcaatc gtcaagggat tgaaacctat agaagacatt gctgatgaac
      961 tgcgcggggc cgactatctg gtatggcgca atgggagggg agcagtccgg ttgctcggtc
     1021 gtgagaacaa tctgatgttg ctcgaatatg ccggggagcg aatgctctct cacatcgttg
     1081 ccgagcacgg cgactaccag gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg
     1141 ccgcatctga ggaacccctg ccttctgccc ttctcccgat ccgggatcgc tttgcagctt
     1201 tgtttcagcg ggcgcgcgat gatcaaaacg caggttgtca aactgactac gtccacgcgg
     1261 cgattatagc cgatcaaatg atgagcaatg cctcggaact gcgtgggcta catggcgatc
     1321 tgcatcatga aaacatcatg ttctccagtc gcggctggct ggtgatagat cccgtcggtc
     1381 tggtcggtga agtgggcttt ggcgccgcca atatgttcta cgatccggct gacagagacg
     1441 acctttgtct cgatcctaga cgcattgcac agatggcgga cgcattctct cgtgcgctgg
     1501 acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt
     1561 ggaacgcgga tggagaagag gagcaacgcg atctagctat cgcggccgcg atcaagcagg
     1621 tgcgacagac gtcatactag atatcaaggg cactgttgca aagttagcga tgaggcagcc
     1681 ttttgtctta ttcaaaggcc ttacatttca aaaactctgc ttaccaggcg catttcgccc
     1741 aggggatcac cataataaaa tgctgaggcc tggcctttgc gtagtgcacg catcacctca
     1801 atacctttga tggtggcgta agccgtcttc atggatttaa atcccagcgt ggcgccgatt
     1861 atccgtttca gtttgccatg atcgcattca atcacgttgt tccggtactt aatctgtcgg
     1921 tgttcaacgt cagacgggca ccggccttcg cgtttgagca gagcaagcgc gcgaccatag
     1981 gcgggcgctt tatccgtgtt gatgaatcgc gggatctgcc acttcttcac gttgttgagg
     2041 attttaccca gaaaccggta tgcagctttg ctgttacgac gggaggagag ataaaaatcg
     2101 acagtgcggc cccggctgtc gacggcccgg tacagatacg cccagcggcc attgaccttc
     2161 acgtaggttt catccatgtg ccacgggcaa agatcggaag ggttacgcca gtaccagcgc
     2221 agccgttttt ccatttcagg cgcataacgc tgaacccagc ggtaaatcgt ggagtgatcg
     2281 acattcactc cgcgttcagc cagcatctcc tgcagctcac ggtaactgat gccgtatttg
     2341 cagtaccagc gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg
     2401 ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca
     2461 acagtgccgc cgatatgatc caactgataa aggaatttga cgctcagggc gtggcagtcc
     2521 ggttcattga tgacgggatc agtaccgacg gtgatatggg gcaaatggtg gtcaccatcc
     2581 tgtcggctgt ggcacaggct gaacgccgga ggatcctaga acgcacgaat gagggccgac
     2641 aggaagcaaa gctgaaagga atcaaatttg gccgcaggcg taccgtggac aggaacgtcg
     2701 tgctgacgct tcatcagaag ggcactggtg caacggaaat tgctcatcag ctcagtattg
     2761 cccgctccac ggtttataaa attcttgaag acgaaagggc ctcgtgatac gcctattttt
     2821 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     2881 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2941 gagacaataa ccctggtaaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3001 acattttcgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3061 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3121 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3181 tccaatgatg agcactttta aagttctgct atgtggtgcg gtattatccc gtgttgacgc
     3241 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3301 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3361 cataaccatg agtgataaca ctgctgccaa cttacttctg acaacgatcg gaggaccgaa
     3421 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3481 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     3541 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3601 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3661 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3721 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3781 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3841 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3901 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3961 ttaacgtgag ttttcgttcc actgagcgtc agacccctta tggcagagca gggaaaggaa
     4021 ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc aatgcgggcg gctggagcat
     4081 ggctttctac gggttcgctg cgagtcttgc cacgccgagc acctggtcgc tttcagctgt
     4141 aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga tggccgaaag tgccgccttg
     4201 ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt gggtgttgag cttcccgttt
     4261 cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc ggaatcctac gctaaggctt
     4321 tggccagcga tattctccgg tgagattgat gtgttcccag gggataggag aagtcgcttg
     4381 atatggcact gttgcaaata gtcggtggtg ataaacttat catccccttt tgctgatgga
     4441 gctgcacatg aacccattca aaggccggca ttttcagcgt gacatcattc tgtgggccgt
     4501 acgctggtac tgcaaatacg gcatcagtta ccgtgagctg caggagatgc tggctgaacg
     4561 cggagtgaat gtcgatcact ccacgattta ccgctgggtt cagcgttatg cgcctgaaat
     4621 ggaaaaacgg ctgcgctggt actggcgtaa cccttccgat ctttgcccgt ggcacatgga
     4681 tgaaacctac gtgaaggtca atggccgctg ggcgtatctg taccgggccg tcgacagccg
     4741 gggccgcact gtcgattttt atctctcctc ccgtcgtaac agcaaagctg cataccggtt
     4801 tctgggtaaa atcctcaaca acgtgaagaa gtggcagatc ccgcgattca tcaacacgga
     4861 taaagcgccc gcctatggtc gcgcgcttgc tctgctcaaa cgcgaaggcc ggtgcccgtc
     4921 tgacgttgaa caccgacaga ttaagtaccg gaacaacgtg attgaatgcg atcatggcaa
     4981 actgaaacgg ataatcggcg ccacgctggg atttaaatcc atgaagacgg cttacgccac
     5041 catcaaaggt attgaggtga tgcgtgcact acgcaaaggc caggcctcag cattttatta
     5101 tggtgatccc ctgggcgaaa tgcgcctggt aagcagagtt tttgaaatgt aaggcctttg
     5161 aataagacaa aaggctgcct catcgctaac tttgcaacag tgccggtaaa tccatgctgg
     5221 ccctgcaact ggccgcacag attgcaggcg ggccggatct gctggaggtg ggcgaactgc
     5281 ccaccggccc ggtgatctac ctgcccgccg aagacccgcc caccgccatt catcaccgcc
     5341 tgcacgccct tggggcgcac ctcagcgccg aggaacggca agccgtggct gacggcctgc
     5401 tgatccagcc gctgatcggc agcctgccca acatcatggc cccggagtgg ttcgacggcc
     5461 tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga cacgctgcgc cggttccaca
     5521 tcgaggaaga aaacgccagc ggccccatgg cccaggtcat cggtcgcatg gaggccatcg
     5581 ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc cagcaagggc gcggccatga
     5641 tgggcgcagg cgaccagcag caggccagcc ggggcagctc ggtactggtc gataacatcc
     5701 gctggcagtc ctacctgtcg agcatgacca gcgccgaggc cgaggaatgg ggtgtggacg
     5761 acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa ggccaactat ggcgcaccgt
     5821 tcgctgatcg gtggttcagg cggcatgacg gcggggtgct caagcccgcc gtgctggaga
     5881 ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta agaacaagca cagcctcagc
     5941 cacgtccggc acgacccggc gcactgtctg gcccccggcc tgttccgtgc cctcaagcgg
     6001 ggcgagcgca agcgcagcaa gctggacgtg acgtatgact acggcgacgg caagcggatc
     6061 gagttcagcg gcccggagcc gctgggcgct gatgatctgc gcatcctgca agggctggtg
     6121 gccatggctg ggcctaatgg cctagtgctt ggcccggaac ccaagaccga aggcggacgg
     6181 cagctccggc tgttcctgga acccaagtgg gaggccgtca ccgctgatgc catggtggtc
     6241 aaaggtagct atcgggcgct ggcaaaggaa atcggggcag aggtcgatag tggtggggcg
     6301 ctcaagcaca tacaggactg catcgagcgc ctttggaagg tatccatcat cgcccagaat
     6361 ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg ccagcgacga ggcggacggg
     6421 cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg tcatgggtgg cggccagcat
     6481 gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg aaaccgcccg cctgctgcac
     6541 cagcggctgt gtggctggat cgaccccggc aaaaccggca aggcttccat agataccttg
     6601 tgcggctatg tctggccgtc agaggccagt ggttcgacca tgcgcaagcg ccgccagcgg
     6661 gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga cggtaaccga gttcgcggcg
     6721 ggcaagtacg acatcacccg gcccaaggcg gcaggctgac cccccccact ctattgtaaa
     6781 caagacattt ttatctttta tattcaatgg tttattttcc tgctaattgg taataccatg
     6841 aaaaatacca tgctcagaaa aggcttaaca atattttgaa aaattgccta ctgagcgctg
     6901 ccgcacagct ccataggccg ctttcctggc tttgcttcca gatgtatgct attctgctcc
     6961 tgcagctaat ggatcaccgc aaacaggtta ctcgcctggg gattcccttt cgacccgagc
     7021 atccgtatga gactcatgct cgattattat tattatagaa gcccccatga ataaatcgct
     7081 catcattttc ggcatcgtca acataacctc ggacagtttc tccgatggag gccggtatct
     7141 ggcgccagac gcagccattg cgcaggcgcg taagctgatg gccgaggggg cagatgtgat
     7201 cgacctcggt ccggcatcca gcaaccccga cgccgcgcct gtttcgtccg acacagaaat
     7261 cgagcgtatc gcgccggtgc tggacgcgct caaggcagat ggcattcccg tctcgctcga
     7321 cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt ggtgtggcct atctcaatga
     7381 tattcgcggt tttccagacg ctgcgttcta tccgcaattg gcgaaatcat ctgccaaact
     7441 cgtcgttatg cattcggtgc aagacgggca ggcagatcgg cgcgaggcac ccgctggcga
     7501 catcatggat cacattgcgg cgttctttga cgcgcgcatc gcggcgctga cgggtgccgg
     7561 tatcaaacgc aaccgccttg tccttgatcc cggcatgggg ttttttctgg gggctgctcc
     7621 cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg cggctgcgct tcgatttgcc
     7681 ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg ctcacaggcc gtggtccggg
     7741 ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc gccgcaggtg gagctgactt
     7801 catccgcaca cacgagccgc gccccttgcg cgacgggctg gcggtattgg cggcgctaaa
     7861 agaaaccgca agaattcgtt aactgcacat tcgggatatt tctctatatt cgcgcttcat
     7921 cagaaaactg aaggaacctc ca
//
