LOCUS       NZ_OZ040720             2630 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30224_1#48 plasmid 5, complete sequence.
ACCESSION   NZ_OZ040720
VERSION     NZ_OZ040720.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068036
            Assembly: GCF_964052815.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964052815.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:50:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,076
            CDSs (total)                      :: 4,964
            Genes (coding)                    :: 4,761
            CDSs (with protein)               :: 4,761
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 203
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 74 of 203
            Pseudo Genes (incomplete)         :: 151 of 203
            Pseudo Genes (internal stop)      :: 37 of 203
            Pseudo Genes (multiple problems)  :: 49 of 203
            Pseudo Genes (short protein)      :: 1 of 203
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ040720.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30224_1#48"
                     /db_xref="taxon:562"
                     /plasmid="5"
     gene            1..705
                     /locus_tag="ABGU58_RS25350"
     CDS             1..705
                     /locus_tag="ABGU58_RS25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<757..861)
                     /locus_tag="ABGU58_RS25355"
                     /pseudo=""
     CDS             complement(<757..861)
                     /locus_tag="ABGU58_RS25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(874..1734)
                     /gene="aac(3)-IId"
                     /locus_tag="ABGU58_RS25360"
     ARG             complement(878..1734)
                     /name="aac(3)-IId"
                     /gene="aac(3)-IId"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(3)-IId"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1877..>2566
                     /locus_tag="ABGU58_RS25365"
                     /pseudo=""
     CDS             1877..>2566
                     /locus_tag="ABGU58_RS25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /name="IS4-like element ISVsa5 family transposase"
ORIGIN
        1 atgaacccat tcaaaggccg gcattttcag cgtgacatca ttctgtgggc cgtacgctgg
       61 tactgcaaat acggcatcag ttaccgtgag ctgcaggaga tgctggctga acgcggagtg
      121 aatgtcgatc actccacgat ttaccgctgg gttcagcgtt atgcgcctga aatggaaaaa
      181 cggctgcgct ggtactggcg taacccttcc gatctttgcc cgtggcacat ggatgaaacc
      241 tacgtgaagg tcaatggccg ctgggcgtat ctgtaccggg ccgtcgacag ccggggccgc
      301 actgtcgatt tttatctctc ctcccgtcgt aacagcaaag ctgcataccg gtttctgggt
      361 aaaatcctca acaacgtgaa gaagtggcag atcccacgat tcatcaacac ggataaagcg
      421 cccgcctatg gtcgcgcgct tgctctgctc aaacgcgaag gccggtgccc gtctgacgtt
      481 gaacaccgac agattaagta ccggaacaac gtgattgaat gcgatcatgg caaactgaaa
      541 cggataatca acgccacgct gggatttaaa tccatgaaga cggcttacgc caccatcaaa
      601 ggtattgagg tgatgcgtgc actacgcaaa ggccaggcct cagcatttta ttatggtgat
      661 cccctgggcg aaatgcgcct ggtaagcaga gtttttgaaa tgtaaggcct ttgaataaga
      721 caaaaggctg cctcatcgct aactttgcaa cagtgccatt tcctcggggt caaagatcag
      781 cgatttggaa cgccgatcgc gcagccgctt agcgagcgtc gtctttccgg cgccgaaagg
      841 tccgttgatc cagattatca ttgtcgacgg cctctaacct gaaggctcgc aagagcgctc
      901 gacggcctcg tgcggaggca cgatcggagt ggttccgaaa tgcttctcaa gataggtgac
      961 gccgaacgtc acgatgtcct gcgcgtcgaa caggtagcac tgagcaaagc ccacgacacc
     1021 ttctcgatgg cgaccgagct tcacgtaagc atttgctata gtttcaaccg catccggctt
     1081 tccttcgata gcaaagcaat cgagaatgcc gtttgaatcg taatccgatg ccgttttcca
     1141 ggcgacttca ccgtctcttc caagcatcgg catctcatac gtcacccacc gtttgttggg
     1201 gatatcggca accgcctcgg cgtagtgcaa tgcggtaacg gagtttagcg gcgcacccaa
     1261 cagcagggcc ttcccgccaa ggcgaacgaa ccgctcgacg ggcgatcctt cccccaaggc
     1321 gtgaccgagt tcgtgaggct ccgtcagcgt ttcagccagc ggaccaaccg cgaccatcga
     1381 tgcatcgggg tgcgcgctgc gccgcgcgcc gggggcttga accagaaatt gattcagcag
     1441 gccgaaccca cggtaagtcc cggctgttgc gggatcgaac ggcagccagg tacggcgggc
     1501 ttcgtcatcc agccgagcgc cattcagagt ctcctcgtag ggtgatcggt cccacgacgc
     1561 gtatcccatc acagtgccag tcggcccaac cgcggagcgt aacgcggcaa cgaccgtctc
     1621 cgctcctcct tcgaccggac caatcgcttt aagtgaggca tgcaccatca agaggtcacc
     1681 ggtttggact ccgagttttt gaagcgcctc cgttattgcc ttccgcgtat gcatcgcgat
     1741 atctcctcta aactgcaaaa cactatacgc tgatgaatcc cctaatgatt ttggtaaaaa
     1801 tcattaagtt aaggtggata cacatcttgt catatgatca aatggtttcg cgaaaaatca
     1861 ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt accaattctg
     1921 ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg cattacttga
     1981 ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag cgagaacaaa
     2041 acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctccaca aagagcgact
     2101 cgctgtatac cgttggcatg ctagctttat ctgttcgggc aatacgatgc ccattgtact
     2161 tgttgactgg tctgatatcc gtgagcaaaa acggcttatg gtattgcgag cttcagtcgc
     2221 actacacggt cgttctgtta ctctttatga gaaagcgttc ccgctttcag agcaatgttc
     2281 aaagaaagct catgaccaat ttctagccga ccttgcgagc attctaccga gtaacaccac
     2341 accgctcatt gtcagtgatg ctggctttaa agtgccatgg tataaatccg ttgagaagct
     2401 gggttggtac tggttaagtc gagtaagagg aaaagtacaa tatgcaggcc taggagcgga
     2461 aaactggaaa cctatcagca acttacatga tatgtcatct agtcactcaa agactttagg
     2521 ctataagagg ctgactaaaa gcaatccaat ctcatgccaa attctatggc actgttgcaa
     2581 atagtcggtg gtgataaact tatcatcccc ttttgctgat ggagctgcac
//
