LOCUS       NZ_OZ040718             5631 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30224_1#48 plasmid 3, complete sequence.
ACCESSION   NZ_OZ040718
VERSION     NZ_OZ040718.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068036
            Assembly: GCF_964052815.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964052815.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:50:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,076
            CDSs (total)                      :: 4,964
            Genes (coding)                    :: 4,761
            CDSs (with protein)               :: 4,761
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 203
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 74 of 203
            Pseudo Genes (incomplete)         :: 151 of 203
            Pseudo Genes (internal stop)      :: 37 of 203
            Pseudo Genes (multiple problems)  :: 49 of 203
            Pseudo Genes (short protein)      :: 1 of 203
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ040718.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30224_1#48"
                     /db_xref="taxon:562"
                     /plasmid="3"
     gene            join(5319..5631,1..11)
                     /locus_tag="ABGU58_RS25290"
     CDS             join(5319..5631,1..11)
                     /locus_tag="ABGU58_RS25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            220..>651
                     /locus_tag="ABGU58_RS25295"
                     /pseudo=""
     CDS             220..>651
                     /locus_tag="ABGU58_RS25295"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            689..1219
                     /locus_tag="ABGU58_RS25300"
     CDS             689..1219
                     /locus_tag="ABGU58_RS25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            1226..1459
                     /locus_tag="ABGU58_RS25305"
     CDS             1226..1459
                     /locus_tag="ABGU58_RS25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1940..2992
                     /locus_tag="ABGU58_RS25310"
     CDS             1940..2992
                     /locus_tag="ABGU58_RS25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            2994..3860
                     /locus_tag="ABGU58_RS25315"
     CDS             2994..3860
                     /locus_tag="ABGU58_RS25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(5063..5254)
                     /locus_tag="ABGU58_RS25320"
     CDS             complement(5063..5254)
                     /locus_tag="ABGU58_RS25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          4888..4969
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          4244..4431
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          2..1563
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5287..5630
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.391"
                     /coverage="97.0"
                     /evalue="6.599999999999999e-67"
ORIGIN
        1 atgatagtta agtttcatgc caggggaaaa ggtggtggca gtggtccggt tgattacctg
       61 ctgggaaggg agcgtaaccg ggaaggggca acggtgcttc aggggaatcc ggaagaggtc
      121 cgggaactca tcgatgccac gccatttgcg aagaaataca cgtctggtgt gctgtcgttc
      181 gcggagaagg agctgccacc gggaggacgt gaaaaagtga tggcgagctt tgagcgtgta
      241 ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt gggtggagca ccaggacaag
      301 ggacggcttg agctgaattt tgtcattccg aacatggagc tacagaccgg aaaacgcctc
      361 cagccgtact acgaccgggc agacaggccg agaattgatg cctggcagac gctggtgaat
      421 caccattacg gtctgcatga ccccaacgcc ccggagaacc gccggacgct gacccttcct
      481 gataacctgc cggaaacgaa acaggcgctt gctgagggcg tcacacgcgg gatagacgcg
      541 ctttaccatg ccggagagat aaaaggccgt caggatgtga ttcaggcgct cacagaggcg
      601 gggcttgaag tggtcagggt gacgcgaagc agtatcagca ttgcagaccc gaacggcggg
      661 aggaatatca gactgaaagg agcgttttat gagcaatctt ttacagacgg gcgcggagtt
      721 cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa atgctgaacg acgagttcag
      781 gaagctcgaa gaatctgtaa acagggaact gacctcaaac gaatctttaa tcaggaacgc
      841 tatcaacgac catacaacag ccctgaaaga actgctggag aggtatcaga aaacgaccgt
      901 cgatacgatg gacgcgcact ggaaaaccgt actgaaaatg agcgtgaaac gctggctgtg
      961 gctgattatc gtctcagtac tgatgtttgc cacgaccggg agtctgctgt ggtaccaggg
     1021 catgaagata aacgcgaata tgaatattct ccgcgagcag aaagagagtc tggagaagct
     1081 gaacgcgaag acatggggcg tgcgttatca cgaggacagc aacggacgtt ttctggtgtt
     1141 gccgaagggg atgaaagccg aaacgaactg gacgaaggac aacggcaaac tgaacgcagt
     1201 gagactggtg caggagtgac agagcatgac ggagctggaa agacatttgc tgaacgcatt
     1261 agagcaactg caacaggact atatgcagcg gctgaacgaa tgggagaacg ccttcgtgga
     1321 attgcagaaa atgttttcgc ttacgcaacg ggacaacgcg atgctgaacg ggcgagtcat
     1381 gcagttgagt cagcaggtgc agcacttgag cgagcggaca gaacgcttga gccagttata
     1441 cagcgagaac aggagataag ggatgaacgg ctgatgcagg aacgcgaaca tgagttatct
     1501 ctggagcggg agcgtcagcc ggaaatccag gagcggacac tggatggccc ttcgctggga
     1561 tggtgaaacc atgaaaaatg acgtgttcag tggattaagg cgggctaatg tggcctgtag
     1621 catctggttg cataggtatt catgcggtta aaatttatca ggtgcgatcg cggcagtttt
     1681 tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcaccga acgcgttttg
     1741 gcggtgcgta caatgcgacg ttatggtaaa tggagcggat tttcaggaag gagtgatact
     1801 aacaactcga acactgtgca aataatgatt tttttaaaat atcggtaacg ataataaatt
     1861 atttcgtttt acgccggtaa taaatataat gtagtttcag tatataaaaa aatatatgag
     1921 gtgtcttgtg ggagctttca tgaatcttca acaacgtata aataaactac cacaattatc
     1981 atccagtttt tcatttggaa aagatattga taatattcat tcatttatct ttaacgaaac
     2041 ttctaaagat aaaattgaag atttactaag aaaatgggtg tcaggcaatc agccctgtgt
     2101 atttggtaaa ttggcaagca agaaaattaa agggcttgat ttccatttat ctattgtaaa
     2161 tagcccccaa ctatataatg atgatggtca tttgtttgat tttttgcgta atgaaagagt
     2221 tcgttttaaa gaacgcgcaa gaagaggaga ggtcagtgcg catctcattt attttattca
     2281 tccacaactt gcctttgctc gtccatcaga agagttagtg gacatacaaa aatatatttg
     2341 ttcgctgcat atgccagagt gctatccaat aaaagaggat gttatttata ctgaaagcgt
     2401 accatttcag gataaagatg ggctaaaaat atataaggct ggtgttaatg ttttttactc
     2461 ttctgctcat agaacaagaa atcatgatag aagaatacca gggggaatac ttatttctgt
     2521 aaatgccccg gggcatttta tgaggctggc gatagaaaaa ggtttctata aagatcaaga
     2581 acaagcgtta gcggatattc gtaacatgac tattcagtct gttggtaagg gtgggtattc
     2641 acatccggaa gggatttcta caacatggca ttcagaatct aatttggatc gttttggatg
     2701 cccagtgcac accggtaata gttcttatta cagcggtttt tatcatactg atgtattaat
     2761 tccaggagaa ttaactaagg atgaaagatt gctccatgaa atagaaaata gtgatccaat
     2821 gatcttcaac tggaatgtat tgttttatgt ttcattagaa gagtttccta tagatgatcc
     2881 ttattatgga gagtttattg gtattcctgt agatgatgca tctatgtttt ttaactcctt
     2941 tcaaccaagg aaatttgaaa acaaacccct ttatgagaaa gaggatgatt aatatgatta
     3001 aaggaatgct ttttgctttt ttagcagcgt tctcatgggg ggcagctatc gtaatgagca
     3061 aaaaaggagt tgaagaacta gatgccggag gggtgttttt ctggcagatt tgtagtgcag
     3121 taatactatc ttggatagtt ctttttataa aaagaaaaaa actcccatta aactcaaaag
     3181 cgatacttgc ttatgcgaca ggcgtttttg aaccattctt agcgtatact tttacactct
     3241 acggacttta ttttatttca gcaggaagcg catcaattat tttttcactt gaatcagtgt
     3301 ttatattggt gttgtctgtt cttatttgct cagttaaaat taattccccg caatatttta
     3361 tattgttcct tataggtgca attgttggca gtattttggt agccttgcca gacatcagta
     3421 atggatcgaa taatattgtt ggatattgtc ttgttgttgc cggtgtgtta tcggcagcat
     3481 tttatgttgt tatatcgtct cgccttatta aatcgtttga gcctatcaca ttattgagcg
     3541 gacagttgac attctccctt atactgtcat cgctctttat ttttatatct aaatcatcaa
     3601 tttatcttcc gttggaatcc gtgattattg ttttttcatc tggaatatta caatattttt
     3661 tagctttttg tttttacttg aactccttgc gttggctccc agttcatttg gcagggataa
     3721 tgttgtactt tattccgcta gttgcattat ttttgtcatg gttgtttttg gatgaacaat
     3781 tcacattctt acagggggta ggatttattg taactgtgct aagtgtatat tttcttaaca
     3841 gaaaatactc agaggagtaa cattctgttc aggattgccc cccggcctcc atcgataccg
     3901 tgccaaatgc acgtaaaaaa cgggagcgtt tctggtgtcg gaatgtaacc aagggggtca
     3961 gaggcgcgct aaaacgctct caggggagtc aaatagagac gtttgtaaca aaaagatcaa
     4021 aggatcctct tgagatcctt tttttctgcg cgtaatctgc tgcctgtaaa cgaaaaaacc
     4081 gccttgcagg gcggtttgat cgaaggttaa gtcagcttgc agactgctta accaggtaac
     4141 tgtcttatgc gagacgagtc accaaaattg ttcttctagt gtagccgcac tccggcagcc
     4201 acttcaagta ctctctaaat atctcgcaca tgacttttac caatggctgc tgccagtggc
     4261 gctttgtggt gtcttttccg ggttggaccc aagagaacag ttaccggaga aggcgcagca
     4321 gtcgggctga acggggggtt cctgcataca gcccagcccg gagcgaactg cctaagtcgc
     4381 cccgagatac cagaagacgt gagctatgag aaagcgccac gtgtcccgta agggagaaag
     4441 gcggaaaagg tatccggtaa tcgatatggc gggaacgtgg gggcgcagga gggagcgatt
     4501 caccggaaac ggtgatgatc tttaagccac gtcgggtttc gccaccgctg gcggatttca
     4561 tggttgagcc gcaaggctcc cacagatgca ccgaaaaagt gtctgttttg tgaagtccgt
     4621 caggggggcg gagcctatgg aaaaagcgcc tccggcgcgg cctttcccgg ggtttcgttt
     4681 tttgcgcaca tgttctttcc tggtttatcc ccgggttctg tggataacag ggttagcgtt
     4741 tttgagaaag gagagtccgc tcgccgcagc cgaacgaccg agcgtagcga gtcagtgagc
     4801 gaggaagcgg aagagcgcct gaactggcat tttctcctta cgcatctgtg cggcatttca
     4861 caccggacat ggcgcacttt tcatacaatc cgcactgatg ccgaatggtt aagccagtat
     4921 acactccgct atcgctacgt gactgggtca gggctgcgcc ccgacacccc aaaggtcaac
     4981 ggcctgacgc gccctggcgg gcttgtctgc tcccggcatc cgctcacaga caagctgtga
     5041 ccgtctccgg gagctgcatg tgtcagcggt tttcaccgtc atccccgaag cgtgcgaggc
     5101 aactgcggta aagctcatcg gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     5161 ccgcgtccag ttcgttgagc ttctccagca gcgttaatgt ctggcctctg atgaagcggg
     5221 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa ggtggggttc
     5281 tgttcatggg ggtaatgata ccgatgaaac gcgagaggat gctcacgata cgggttactg
     5341 atgatgaaca tgcccggtta ctggaacgct gtgagggtaa acaactggcg gtatggatgc
     5401 ggcgggtctg cctgggtgag ccggttgccc gttccgggaa gctgccgaca ctggcaccgc
     5461 cgttactgcg tcagctggcc gccatcggga ataaccttaa ccagacagcg aggaaggtga
     5521 acagcgggca gtggtcttcc ggtgaccggg ttcaggtggt ggccgcactg atggccatcg
     5581 gggatgagct gcgccggctg cgtctggcgg tcagggaaca gggggcgcgg g
//
