LOCUS       NZ_OZ040492             6769 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30602_1#64 plasmid 3, complete sequence.
ACCESSION   NZ_OZ040492
VERSION     NZ_OZ040492.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068046
            Assembly: GCF_964051065.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964051065.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 12:03:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,162
            CDSs (total)                      :: 5,047
            Genes (coding)                    :: 4,884
            CDSs (with protein)               :: 4,884
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 70 of 163
            Pseudo Genes (incomplete)         :: 117 of 163
            Pseudo Genes (internal stop)      :: 32 of 163
            Pseudo Genes (multiple problems)  :: 47 of 163
            Pseudo Genes (short protein)      :: 1 of 163
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ040492.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30602_1#64"
                     /db_xref="taxon:562"
                     /plasmid="3"
     gene            1..435
                     /gene="psiB"
                     /locus_tag="ABGU68_RS25720"
     CDS             1..435
                     /gene="psiB"
                     /locus_tag="ABGU68_RS25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="WP_000845922.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPDN
                     WTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVVQV
                     QRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQV"
                     /name="psiB"
     gene            432..1218
                     /locus_tag="ABGU68_RS25725"
                     /pseudo=""
     CDS             432..1218
                     /locus_tag="ABGU68_RS25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /name="plasmid SOS inhibition protein A"
     gene            1408..>1521
                     /locus_tag="ABGU68_RS25730"
                     /pseudo=""
     CDS             1408..>1521
                     /locus_tag="ABGU68_RS25730"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
                     /name="DUF5431 family protein"
     gene            1466..1588
                     /locus_tag="ABGU68_RS25735"
     CDS             1466..1588
                     /locus_tag="ABGU68_RS25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="WP_223195199.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK"
                     /name="Hok/Gef family protein"
     gene            complement(<1889..2186)
                     /locus_tag="ABGU68_RS25740"
                     /pseudo=""
     CDS             complement(<1889..2186)
                     /locus_tag="ABGU68_RS25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(2610..3614)
                     /locus_tag="ABGU68_RS25745"
     CDS             complement(2610..3614)
                     /locus_tag="ABGU68_RS25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744774.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="WP_024257664.1"
                     /translation="MYQLGIDVSKNTLDLCLLREGIKGRIKTRKLKNTADAAKVVAEWL
                     KKQSCELGEVHIIMEATGVYHEPLAYGLHFAGARISLANPHRTREFARGMNILTKNDKV
                     DAWMLACYGSLKEPEAWTPPPESVRYLRALLRRRDALVADSTREKNRLEKSVFTDSPDA
                     VRASIENMLQELSNEIEHLDVLIQEHIKTHPELNQDFRLLTSIKSVGYLLGLNMLAILK
                     SHSFESAEQAAAFLGVVPVERRSGTSVKGKPKLSKIGPPEIRTKLYLAALCGLRFNPVM
                     KQMYTKLCQRGKSKMCAIGALMRKLVHWCYGVLKTQLPFDAEYNQQSYYKTIF"
                     /name="IS110 family transposase"
     gene            3865..>4362
                     /locus_tag="ABGU68_RS25750"
                     /pseudo=""
     CDS             3865..>4362
                     /locus_tag="ABGU68_RS25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /name="single-stranded DNA-binding protein"
     gene            4460..4693
                     /locus_tag="ABGU68_RS25755"
     CDS             4460..4693
                     /locus_tag="ABGU68_RS25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="WP_000005988.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLVV
                     RNNGAMVWRTWNFEDGAGYWMNHAIRDFGILK"
                     /name="DUF905 family protein"
     gene            4757..6715
                     /locus_tag="ABGU68_RS25760"
     CDS             4757..6715
                     /locus_tag="ABGU68_RS25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="WP_000117202.1"
                     /translation="MSVTESKTKTERKSSRKPSKEQETALSALLAQTAEVSVPLASLIK
                     SPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLAER
                     GIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIGDLL
                     GYSPRYVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQSGWG
                     GKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRVALDAA
                     LLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPDAVLTEAEEERLNEL
                     MTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRTGSGVVVSWRYGNVCVQRGVQ
                     LRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPDKSLALLAW
                     TLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARLAALLPEGWS
                     RDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAIGFHMRDWWQP
                     TKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEHHMKDNRWVPGWMCAP
                     HPQTDTTERTDNLADAA"
                     /name="ParB/RepB/Spo0J family partition protein"
ORIGIN
        1 atgaaaactg aactgaccct gaatgtatta cagaccatga acgcacagga atatgaagat
       61 atcagggctg ccggaagtga tgaacgccgt gagctgacac acgccgtgat gcgggaactg
      121 gatgcgccgg acaactggac gatgaatggc gagtacggca gcgagttcgg cggctttttc
      181 cccgtccagg tccgtttcac gcctgcccac gaacgttttc acctggcgtt atgttcaccg
      241 ggtgatgtct cccaggtctg ggtgctggtt ctggtgaatg ccggtggtga gccgtttgcg
      301 gtggttcagg tacagcggcg gtttgcaccg gaagcagtca gccattcact ggcactggcg
      361 gcgtcactgg atgcgcaggg gtacagcgtt aacgacatca tccatatcct gatggcggaa
      421 ggaggtcagg tatgaacgca cgttcacggg cactggttcc cctcagcaca gagcaacagg
      481 ccgcatggcg ggcggtggcg gagacggaaa aacgccgcca tcagggaaac acgctggcgg
      541 aatatccgta tgcgggagca tttttccgct gtctgaacgg aagccgccgg atttcgctgt
      601 ctgacctgcg atttttcatg ccgtccctga ccgcagaaga actgcacggc aaccgtcttc
      661 agtggctgtg tgccatcgat gtgctgattg aaacacaggg cgaggtctgc ctccttcccc
      721 ttcccggcga tgcagcagaa cgcttgttcc cgtcggtgcg gtttcgtgtc agggaacgca
      781 gtcgccataa atccgcgctg gtcatgcaga agtacagccg ccagcaagcg agggaggcag
      841 agcagaaagc ccgtgcgtac caggtgctgg ttgctcaggc tgaaatcgaa ctggcatttc
      901 attcaccgga aacagtcgga agctggcatg cacgctggag tgaccgggtg gcagagcatg
      961 acctggaacc cctgttctgg cagtggggcg agcgttttcc gtcactggcc ggaatggaac
     1021 gctggcagtg gcaggatatg ccattctggc aggttatcgc ggaagccagt ctggcagcca
     1081 gagaggccgg tcatgctgtc cgtgagatgg agcggtggat ggtgccgaac aaactccggg
     1141 aggcagcgtg atgcggcaat aatcacacgg attacccgtg aactggttga acgaccagat
     1201 tattttcagg gaaagtgagt gtggtcagcg tgcagggata tgagctatga tgtgcccggc
     1261 gcttgaggct ttctgcctca tgacgtgaag gtggtttgtt accgtgttgt gtggcagaag
     1321 gcagaaagcc ccgtagttaa tttttcatta acccacgagg ccccctgtat gtctgatcaa
     1381 caacagtatg gcctcttacc gtgcttaatg caaggaggag taaaccatga aactaccggg
     1441 aaacgccctt atctggtgcg tattaatcgt gtgctgcacg ttgttaatat tcacactcct
     1501 gacccggaat cgtctgtgcg aagtccggct gaaggacgga tacagggagg ttacggcaag
     1561 tttggcttac gaatccagcg gtaagtagca acctggaggc gggcgcaggc ccgccttttc
     1621 agggctgatg ctggtctgac tgcactgaag cgcctttata aaggggctgc tggttcgccg
     1681 gtggcccctt tctccttgct ggcattgtgc gggcatgaac aaccagactg ccaggaaggc
     1741 cgtttccctc cgggaacggg gatgaacgtg cgcctgcggc gcccgttctt ttcccccgcc
     1801 ttctctgttt atgacctgtc agaaatcaca tcaccttccc tttgattatt ctctttttcc
     1861 tcctgttctg actctgatta ctgctgccct gattattgtc tgttttgttg ttctcctgct
     1921 tatgcctttt ccgggcgtcc ccctggcggg gcgggctgtc gtgaacggag ccagcggagc
     1981 tgtctccgcc ctggcgggct tccatccctg acgcgaaacc ccgggcttgc agcgaccgtt
     2041 ccggtcgcgc cgcctctgtc gcgccgtggc ctgcgctccc ggcatcccgg caaaaggccg
     2101 ccgggatgta aggggcaacg ccgtttcgca aaccgcgctt cacgtcgttg cggcgggttc
     2161 ctgcccgttg ttgtgtccgg tgccattcac ctgtttaagc ccctgtcgcc gggagctgat
     2221 acagccacaa cctgccgcac tgactgtcca gggtaaatga acagcccaca aaatttttta
     2281 cccaaaaccc ggtaaaaaat tttgcgttct gcccaggaca ggtgcgtcag gccgtggcag
     2341 tgatgcccct tgcggcgaag gttctcaaac aggtgatggt gaataccgga gggaacaaac
     2401 ggcaggtccc cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacagct accgtgttta
     2461 gtcaagaaaa ttatgtgatg tttgaacatt ccattttcag gagaaatttt ctctgagttt
     2521 ttataaaaag agcagatgct cagttaaata ccatgataga aggaaaaaga tacaaacatg
     2581 ttgcttcagt attgctccga tggtgttgat taaaatattg ttttataata actttgttgg
     2641 ttgtattcag cgtcgaatgg taactgcgtt ttcagcacac catagcacca gtgcacaagt
     2701 tttcgcatta atgctccaat agcacacatc tttgactttc ccctctggca taacttcgta
     2761 tacatctgtt tcattacagg gttgaatctc agcccacata aagcggcaag ataaagtttt
     2821 gttcttatct ccggtggtcc aattttagat aatttaggtt ttccctttac agatgtgcca
     2881 gatctccttt ctaccggaac tacaccaagg aatgctgctg cctgctctgc tgattcgaat
     2941 gaatggcttt tgagtattgc cagcatattt aatcccagca ggtaaccaac agattttatt
     3001 gatgttaaca gtctgaaatc ctggttgagc tcagggtgag ttttaatatg ttcctggatc
     3061 aggacatcaa gatgttcgat ctcattgctc agctcctgaa gcatgttttc aattgacgcc
     3121 ctgacggcat cagggctgtc tgtaaaaaca gatttttcaa gtctgttttt ttctctggtg
     3181 ctgtctgcaa caagagcgtc tctgcggcgt aaaagagctc taagataccg cacagattct
     3241 ggtggtggag tccatgcctc aggctcttta agggagccat aacaagccag catccaggca
     3301 tcaactttgt cattcttggt caggatattc atacctctgg caaattccct tgtgcggtga
     3361 ggattggcta aagaaattct ggcgccagca aagtgaaggc cataagccag aggttcatga
     3421 tataccccag tggcttccat gatgatatga acttcgccca gttcacagga ttgcttttta
     3481 agccactctg ccacgacttt agccgcatca gcagtatttt ttagcttacg agttttgatc
     3541 ctccctttga tgccctcccg taagaggcat agatcaagag tgtttttact gacatcaata
     3601 cctaactggt acatcggcat tttttctcct ttcacataac catcacgcca atttgtcttg
     3661 ttcatgcaga gtcttagctc ttggcaaccg ttcaatgtta atgctggcgt gaaaggtgaa
     3721 gcagaggact atagctgttg acgaaggtta ttaccttaga tgatggggta ggttctctcc
     3781 gcttcatgcg atggtagcta atcatcgctc gttgattaca tacaagatga tggggctgaa
     3841 aacatcaaca gaaggagaca catcatggca gttcgtggca ttaacaaggt cattctcgta
     3901 ggacgcctgg gaaaagatcc ggaagtccgt tacatcccca acgggggcgc agtggcaaac
     3961 ctgcaggtgg ccacatcaga aagctggcgt gacaaacaga cgggggagat gcgggagcag
     4021 acagaatggc atcgtgtggt gctgttcggc aagctcgcgg aagtggcagg tgaatatctg
     4081 cgcaagggcg cgcaggtcta catcgaaggt cagcttcgca cccgtagctg ggaggataac
     4141 ggtatcaccc gttacgtcac cgaaattctt gttaagacca cgggcaccat gcagatgctg
     4201 ggacgtgtcg caggtgctca gactcagccg gaagaggggc aacagttcag cggtcagtct
     4261 cagccggaac cacaggcgga ggccggtacg aaaaaaggag gcgcaaaaac gaaaggccgt
     4321 ggacgtaagg ccacgcagcc ggagtcgcag ccgcaaccgc cggagagtga cgattacggg
     4381 ttttcagatg atacccgttc tgaacgactg actgtgacaa ccgctccgtc ctgtgcgggg
     4441 catcaccgga gagatgagga tgagcgaata tttcagaata cttcaggggc tgcctgacgg
     4501 tccttttacc cgcaaacatg ccgaagctgt tgccgcacag taccggaacg tctttatcga
     4561 ggatgaccac ggcgaacagt ttcgcctggt tgtccgtaat aacggtgcaa tggtctggcg
     4621 tacctggaat tttgaggacg gtgccgggta ctggatgaac catgccatcc gtgatttcgg
     4681 cattcttaag taagagaggt gccggacggg aatgcgtccg gcagacatac aaccatcccg
     4741 gtcaaaggag aaatatatgt cggttacaga gtctaagaca aaaacggagc gtaaatccag
     4801 ccgtaaaccg tcaaaagagc aggagactgc cctgtctgcc ctgctggcgc agacggcgga
     4861 agtgagcgtg ccgctggcct cactgattaa atcaccactg aatgtgcgca cggtgccgta
     4921 ttctgcggag tctgtcagcg aactggcgga gtccattaag ggcgttggcc tgttgcagaa
     4981 tctggtcgtg cataccctgc ctggtgaccg ttacggtgtc gccgcaggtg gtcgccgact
     5041 ggctgcactc aacatgctgg cagagcgtgg catcattccg gctgactggc ctgtccgtgt
     5101 gaaggtcatt ccgcaggagc tggcgacggc ggcatcaatg actgaaaacg gtcatcgtcg
     5161 ggatatgcac cctgctgaac agattgccgg attccgcgca atggcgcagg aaggcaaaac
     5221 acctgcacaa atcggtgatt tgctgggcta ttcaccccgc tatgtccagc gaatgctgaa
     5281 actggctgac cttgcgcctg tcatcctcga tgcgctggca gaagaccgca tcactaccga
     5341 acactgtcag gcgctggcgc tggagaacga caccgcgcgt caggtacagg tgtttgaagc
     5401 cgcctgtcag tcgggatggg gcggaaaacc ggatgtgcgg gttatccgca atctgattac
     5461 ggaaagtgaa gtggcggtgg cggggaacag taaattccgc ttcgtggggg ctgatgcttt
     5521 ctcacctgac gaactgcgta ctgatttgtt cagcgatgac ggggacggtt atgtcgaccg
     5581 cgtggcgctc gatgctgccc tgctggaaaa actccaggct gtcgctgaac accttcggga
     5641 agccgaaggc tgggaatggt gcgccggacg catggagcct gtcggtgagt gccgtgagga
     5701 tgccggaaca taccgctgtc tgccggagcc ggacgcggtg ctgacggagg cggaagaaga
     5761 acgcctgaac gaactgatga cgcgttacga cgcgctggaa aaccagtgtg aggaatccga
     5821 cctgctggaa gcagaaatga agctgatgcg ctgcatggcg aaggtcagag cgtggacgcc
     5881 ggagatgcgt accggaagcg gtgtggtggt gtcctggcgt tacggtaatg tgtgtgtcca
     5941 gcgtggtgtg cagttgcgca gcgaggatga cgcggctgac gacgctgacc gcacggaaca
     6001 ggtgcaggag aaagcatcag tggaggaaat cagtctgcca ctgctgacga aaatgtcttc
     6061 cgaacgcacg ctggcagtcc aggcggcact catgcagcaa ccggacaaat ccctggcact
     6121 gctggcatgg acgctctgcc tgaatgtgtt tggcagcgga gcgtacagta aaccagcaca
     6181 aatcagcctg gaatgtaaac attattcgct gaccagcgat gcgccgtcag ggaaggaagg
     6241 tgccgcgttc atggcgatga tggcagaaaa agcccgtctt gcggccctgt taccggaggg
     6301 atggtcacgg gacatgacga cgttcctgtc actcagtcag gaggtgctgt tatccctgct
     6361 cagtttctgc accgcgtgca gcatccacgg cgttcagacc cgcgagtgtg gtcacacgtc
     6421 acgcagcccg ctggacacgc tggagagcgc catcggtttt cacatgcgtg actggtggca
     6481 gccgacaaaa gcaaacttct tcggacacct gaaaaagccg cagattatcg cagctctgaa
     6541 tgatgccgga ctgtccggtg ccgcacggga tgcagagaag atgaagaaag gtgatgcggc
     6601 tgaacatgca gagcaccata tgaaagacaa ccgctgggtg cctggctgga tgtgtgcacc
     6661 acatccacag acggatacca ctgaacgcac cgataacctg gctgatgccg cctgatgaac
     6721 aaccacaccg ccccgccgga gacggggcag cagcaaggga gataccgtg
//
