LOCUS       NZ_OZ040270             5167 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30821_1#88 plasmid 4, complete sequence.
ACCESSION   NZ_OZ040270
VERSION     NZ_OZ040270.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068099
            Assembly: GCF_964049545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964049545.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:52:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,015
            CDSs (total)                      :: 4,904
            Genes (coding)                    :: 4,704
            CDSs (with protein)               :: 4,704
            Genes (RNA)                       :: 111
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 70 of 200
            Pseudo Genes (incomplete)         :: 148 of 200
            Pseudo Genes (internal stop)      :: 35 of 200
            Pseudo Genes (multiple problems)  :: 43 of 200
            Pseudo Genes (short protein)      :: 1 of 200
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ040270.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30821_1#88"
                     /db_xref="taxon:562"
                     /plasmid="4"
     gene            1523..2437
                     /locus_tag="ABGU63_RS25020"
     CDS             1523..2437
                     /locus_tag="ABGU63_RS25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(2641..3039)
                     /locus_tag="ABGU63_RS25025"
     CDS             complement(2641..3039)
                     /locus_tag="ABGU63_RS25025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            3216..4142
                     /locus_tag="ABGU63_RS25030"
     CDS             3216..4142
                     /locus_tag="ABGU63_RS25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            4145..4678
                     /locus_tag="ABGU63_RS25035"
     CDS             4145..4678
                     /locus_tag="ABGU63_RS25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(4744..4953)
                     /locus_tag="ABGU63_RS25040"
     CDS             complement(4744..4953)
                     /locus_tag="ABGU63_RS25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          1524..2298
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          3217..4139
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtatggaa tggaagtgag ctttaaggtt tcagctctaa aaaataaaat atttattagt
       61 tggctggttg ttgttcttgt tctgtatgta tataaacact atcgacatat tatgaccggg
      121 aacattcctt accccctctg cgctcgcaga ggtttttttt gtcattaatt aataatgaac
      181 tgaaatgcag ttccgatacc tccatttgca cattatccct gacatatggt accggaaccg
      241 cattaatttt atgttataag agtttatttt taatgtccag atggtttaat agtatttata
      301 tgtaattagc tgtatggata gtactggttt tattgtataa cttgtcgttt ttttttattg
      361 tttttgtgtg gtgtgctaat ctttaagtta taaaaaaacc ccttggaata caggggcaaa
      421 agtaaggaaa acaaatggat gctcagtttg tgtgacactt taaactaatg gaactataga
      481 gtggaggttg ttacagtcgc ttttataaaa cagcaaccag tttttttcaa tagtctgatg
      541 atggttgtga cagttttgtg atattgttca aaatgtttct tgttgaatgt ttgttttttg
      601 gtctattggt gtctggtttt attttttctg ctattacata tcaagaaatg tgattggtgt
      661 agcaagagtg gaatgcgatg tggataaact aattggttgt cgaatctaat ttacttagaa
      721 aactacactt gtttttgtgt atgaatttag tgaatttgtc tgtctatgtg ttttcgtgca
      781 tttataaatc cttcgtcagg atcttaaatt tagtgttgaa tatgtagggc ctgtacggta
      841 attattattt agcgtagcta cttcatcgtt gttcgaacac aaccaacaac ctgtaattgt
      901 tgtttctctt ggttccccac ttttgtgggg ttttttatct cttccgtgca tcgtaaagcg
      961 cggactcctt ctggaaaggt tgttttaggg catcactgag gcattttttg cgtttgtgaa
     1021 tattgagcat ccgataacta aaatatttcc catagactct gattcttgcc accgacccca
     1081 ggtcggtttt tttttgtcct ggcgagatgt gaagtttgga aacaggaaac cccgtcagag
     1141 cgccacgcac caggggagaa aacttgtcgt tttgagccgt tcggtgttca gaacgcacga
     1201 aaccgatcgc acatcgcttt cgtgcatagt tatgcaacgc cctgaaaacg attctgacgc
     1261 gtttttctgg tttggcctgg tgttttcttg tctttttgcg ttttttgcgc cagaaagcga
     1321 ctgagggcgt tttaaggggg gcgtacaacg ggagttatgg taaatggatc gggttttcgg
     1381 gaaggttcga caggatttgc cgttgggtgt agtgtaagcg actgaaaaac aaacgcccct
     1441 gaaatcatgt ccagatccgg caagattcaa catgaaatac agagggcgtg taggtcgcat
     1501 aacccggaga gaatcgtaac acatgagcgc cgcgcttcaa tacttcgacg aaaatttacc
     1561 ccatcgtccc tatcacacgg atgatctcgc ttttggtctt cgcatctccg gcaaagggcg
     1621 tgcgcttctt gcgcggtaca tacagcagaa ccagcctcat gcgcagttct ggctggtttt
     1681 tgacgttgac cgcgaggggg ctgcgattga ctggagcgac cggaacgctc ccgcgccgaa
     1741 catcaccgtt aaaaatcctg tgaacggaca cgctcacctg ctctacgcac tcaacatcgc
     1801 cgtgagaacc gcgcctgatg cctcggttaa ggcgctgaaa tatgccgcag cggttgaacg
     1861 ttcgctgtgt gagaaacttt gtgcggatgt gaactacagc ggcctgattt gcaaaaatcc
     1921 gttccacctg gaatggctgg tgatggagtg gcgcgaggaa gcctataccc tcgatgaact
     1981 ggctgattat ctcgatttga gcgcctcaga gcgtcgtagc atcgataaac attacgggat
     2041 ggggcgaaac tgccacctgt tcgaaatgac gcgcaaatgg gcttacaggg cgattcgtca
     2101 gggctggcca gcgttctcac agtggcttga agcggtgatc cagcgtgtcg aaatgtacaa
     2161 tgcatcgctt cccgttccgc tttcactggc tgaatgtcgg gctattggca agagcattgc
     2221 gaaatacacg cacaggaact tcacgccgga aactttcgca cagtatgtgg ctgatacgca
     2281 cacgccagaa attcaggctg cacgcggtcg caagggcggt tctaagtcta agcgcagcac
     2341 agtagctaca tctgcacgca cgctgaaacc gtgggaggca ttagggatca gtcgcgcctg
     2401 gtattaccaa ctgaaaaaac gaggtctcgt agagtagacc aaataagcct atatcagata
     2461 acagcgcctt tctggcgtct ttttgagcag caggtttttt gccgtttttc cccgttcagc
     2521 cctgcaatgg cgcggctttc cgcgtgattg tggtggtagt gctcgccgca gtctcatgac
     2581 cgagcgtagc gagcgaatga gcgaggaagc accagggaat cggaacacgg atggaggcac
     2641 ttacgcgtct gggtttgggg atgtctccag atcgtactta agttccttaa gtgcttgtaa
     2701 tatcctgtct tttttatagc atagctcgcc tgtttttgtg tctaccagtc ctgcttcgat
     2761 cagtagccca gccgcgagaa agagccggtg attgcgttcc cgtcgttgtt tttttccctc
     2821 gattcgtttt tggaccgcct gttttcgttc gctctcctgt gcctgtaagt cgtttttttc
     2881 cgctgttgcc agtacgtgga gaagtttgcg ttcctgttct gtgagtccgg cttcgttgtc
     2941 gatcaggtca ataatctgtc tctgtcgtgc ggtctgttct ccgagcttcc tgatgaatcc
     3001 gatccgtttc ttcagccatt cgatgttgct gatgcgcatg ttcgtgatgc tccctggtgt
     3061 ttttttgcgg tgtgttgtgg tcgtgcagta tatcgcttgc ggtcggcggg tgcagggggt
     3121 atcttccgtg ctaattcgta aaattagaag gaagcgcact tatatgtttc tctccttcgt
     3181 cgagaaacag tgcgctcctg cagagggctg aaaccgtggc aacctatcac ctcagcgtta
     3241 aatttggtgg caaggggcag gctgcaaacc acgccgatta catcgagcgc aaagagaaat
     3301 accgcgaccg tcaggatttg gaatattccg cacacggaaa catgccggaa tgggccaggg
     3361 ataatccttc ccatttctgg caggcggctg accagtttga acgtgcgaac ggttcgacct
     3421 atagggaact tgaaattgca ttaccccgtg aactgacacc ggaacagcgc cttgaactgg
     3481 tgcaggattt tgtacgtcag gaagctggcg aacggcacgc ctggagtttt gccattcaca
     3541 atccgaaagc gagcattgac ggcggcgaac agcctcatgc gcatatcatg atgagtcagc
     3601 gtgtgaatga tggcattgaa cgtacaccgg aacagtattt ccggcgctat aacgccaggt
     3661 atcccgagcg gggaggtgcg aaaaaggatt ccggaagcct gactctcact cagcaaaaag
     3721 agcagttgcg ggaattgcga aaacgctggg aggtgaagca caacgaacat atgagaaagc
     3781 atggctttga aaggggcttc attgactgcc ggacgctgaa agaacagggc attgaacgac
     3841 gtcccgaagt gcatctgggt tttgaagctg ccggacggct tgatgatgcc cagcgtcatg
     3901 acatcatgca aaaacggtct gaaccggact attcccgcca tcttgaacag gcgcgaacgg
     3961 tcgggcatga catcagggaa tggctggaca ctccggttaa gcaacatgaa ctggtcagaa
     4021 ggctgcaaca tgatgttgcg cgggagcatg gctttgacat gagagacaga gtgagcagac
     4081 agaaccgaac tgcacctgaa cgggttaaat caccgtcacg tggattttct atgggattct
     4141 gaacatggcc agcacagaag aaaaattact tacgctcctg ttatcggtgg agcatctgca
     4201 acataccgca atggagcaac agaaagctgt tgctgattct ctggctgtgg caagggaaac
     4261 tcttcgcgga tatcgggata tttctgacgg tgtggcaaaa caggtcaggg atgaagtcag
     4321 aaatgaggtg cagaagctgg acgtggcagg actgataggt gagcgcatca gccaggattt
     4381 tcagcggctg gaagagtcag tcgcaaacat gaaggcaaac gcggatgccc tgaacaggga
     4441 tattgcgaca attcacagca atatcgtgca ggaatacaac acccttaaag ggtatagctg
     4501 gaaatggctg ggtggggtgt cagtggcttt tctggttatg ttctgtgtgc tcggatgggg
     4561 aatgaaaaca atactcgaca ggaactacaa cgccacagcg gcggtgtatc agaaagttgt
     4621 gacactggaa gataaactgg tggagaaacc tgtcacgggg aagagccgga aaaactgacg
     4681 cgcttcgctt gtccaacgcc tcacgacccg cgaaagaatt tcgcgaaccg ttcaccgttt
     4741 ttttcaatat gattctgtct gctttggtcg ataaagcaga cgatagtatt ccagccttcg
     4801 tatatataac ggtacgtgct catcagtaag agctggcggt attgtgttat cagggggaag
     4861 ttttttcaga tactcacgaa atgcctggct tgatgccata aaatctacag tagcatcgat
     4921 aatatgtggg gtgttttcac ctaattttgc catgatgata atccttatat tatcacggtt
     4981 tgtgaatgat agggtgtcac tattatttat gcgttagtga accagaaaaa atcccccgtt
     5041 ggcatagggg gatatgtaag gagaaataac ttcgcaggtg ttcttgtttg tataacgtga
     5101 aaagactgca cgcaacttaa taagtctgcc gtacttactt tcagttttca acataaggtt
     5161 tataaca
//
