LOCUS       NZ_OZ040210             4071 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30224_1#333 plasmid 3, complete sequence.
ACCESSION   NZ_OZ040210
VERSION     NZ_OZ040210.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068084
            Assembly: GCF_964049315.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964049315.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 12:02:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,915
            CDSs (total)                      :: 4,802
            Genes (coding)                    :: 4,596
            CDSs (with protein)               :: 4,596
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 80 of 206
            Pseudo Genes (incomplete)         :: 150 of 206
            Pseudo Genes (internal stop)      :: 46 of 206
            Pseudo Genes (multiple problems)  :: 57 of 206
            Pseudo Genes (short protein)      :: 1 of 206
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ040210.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30224_1#333"
                     /db_xref="taxon:562"
                     /plasmid="3"
     gene            1..2148
                     /gene="mobQ"
                     /locus_tag="ABGU82_RS24565"
     CDS             1..2148
                     /gene="mobQ"
                     /locus_tag="ABGU82_RS24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_021526521.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRENRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2405..3106)
                     /locus_tag="ABGU82_RS24570"
     CDS             complement(2405..3106)
                     /locus_tag="ABGU82_RS24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3639..3893)
                     /gene="mobC"
                     /locus_tag="ABGU82_RS24575"
     CDS             complement(3639..3893)
                     /gene="mobC"
                     /locus_tag="ABGU82_RS24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          2..359
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3895..4071)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          complement(2406..3106)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..2145
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.106"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcgattt atcatctcag catgaaaatt atctccagaa gcagcggcta cagcgccgta
       61 gcttccgctg cctatcgttc cggttcactg atgcttgatg agcgtaccgg actgactcac
      121 gactacaccc gcaaaagcgg tgtggctgag gctgtgattc tgacccccgc caccgcccct
      181 gcctggtgta cgaaccgtgc cgaactctgg aatgccgttg agaaggccga acgccggaag
      241 aactcacaac ttgcgcggga aattgaactc gccatccccc acgaactgcc gcaggatgcc
      301 gcacgggaaa ccgttctcgc ctttgtcaga gaaaactttg tcagtcaggg catgattgct
      361 gatgtggcgt tccatcacat ggataaaaca aatccccatg cgcacatcat gctgaccacg
      421 agggctgtcg gacctgctgg gttcagcgga aaggtcaggg actggaacga ccggacacat
      481 gccgagacgt ggcgggcatc atgggctgac catgcaaaca gggcactggc gaacgccggt
      541 tatcaggaag agatagacca tcgttcatac gagcgacagg ggcttgagaa aacgcctggt
      601 attcacctcg gaaagtctgc ctgtgcgatg gaaacacgcg gtatcgaaac agaacgcggc
      661 gaacagaatc gtctgattaa caggcttaac ctggaaatac agatttcccg caccgaactc
      721 aggaacggag ggctgacaga gcctgcccgc cgtgtggctg accttctgaa tatcgggctt
      781 cctgacaacg caacttcgta cacactacgt gacattatcg aagcgttgcc gaaggacagt
      841 aactcggcat ggaaactcac cagtaatgcc atgtcgatga tggcagatat gcaggcgacg
      901 cgcagacgct gggaagagct gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc
      961 ctgaaaaaat catcgccgtt ttcgtccggt ttttcccgta ttcccctgat gcaatgggcg
     1021 gcaccggaat accgtagtga gcagggaaaa atccagtctc tgagccagca aatggaaaaa
     1081 ttacgacagc attaccggaa ggtggagaaa caggacattc ccgcgagtca gatggcattt
     1141 gaaaaacagt ggcagcagtg gggggaatcc ggccttgcag ctctcagagc ccagttgctt
     1201 aaacgtgaag aggaattgcg ccggaaggaa caggaagata ctgaacgtag tcgcgcggag
     1261 cagctcagga aaaatgacaa tgccgttatg gattcagggg tgctgagagc agtcgttacg
     1321 gggtatggcg aggctcccat gcctggtaac ggggaaatga cctgttacct tctgcttcac
     1381 aaccggaacg gagaatatac gctctggggg aacgagctgg agaaataccg caccagaatc
     1441 tttgagagtg ttgatttaat gcgtgacagg agtggttata tctgtgaccg aagcgaaatc
     1501 ggccaacgtc cgccactgca acgtgtttat tcctctgcaa cctttgagca actgctgacg
     1561 caggtttgcc agacatggcc gcaacatacc cgcaatctcc gccagccgaa gacatggccg
     1621 gagtctttct gtcttggaga agaccgccag ccagccatgc catcaccagc ggcaaggaag
     1681 gtggatttta tgcagggacg tctgccaccg acgctgatgc cggtcatgag ttctgtcgac
     1741 cgggaaaacc gtcagcttca gcttttgctg gtcatgggcg tggatgactc tctgggcggt
     1801 gttatccggc ttaatggtac gctgtatccc gcgtttgccg ttccctcagc cgataacagg
     1861 catctggtca tcagtgcgct gaccgataag gggttacggt atgcagggta tggtgttgcc
     1921 gtaaatcacg atgcagacag ccacatatca cctgcaccag aactgatgca gtttcatctg
     1981 aaaacccgtg aggcaccgct ttttgctgcc gtcaacacac cagaaaaaca gccggatcat
     2041 ctgttccgct cgctgggttt taatcgtacc tgggaggaat ggcagcgcga ggaagatgca
     2101 cgaacacaca ccaccgagag gagacatgat cgcggttggt cacagtgaat gtgaacaata
     2161 ttttgttttg tttaacatcg tgttatgttc gtcgatgttc ttcgatgttc atcgacaggg
     2221 atgaatgcaa gaagcacact cacataacgc aagcatagtt ttgtttttcc gcccccaaaa
     2281 attgggggtc ttttttcgct gcatcagagc gtttttgacg caatttttca cttcaatagt
     2341 accttcgcac tactaacaca aaaaaaacgc agcagggagc gttttaggtg ccttaaaatc
     2401 gattttagat cgtcatcttg atctgtttgt cttccttgaa gcgaaaatgc agggctacaa
     2461 ccgtgcgccc ctttttgatc gtctcaacag ttacaaccag atcgcttttt tcgttcagtt
     2521 cggcgatggc tggcttgatt atcatcttat tcaagtcttt gaattgttga tatttatctt
     2581 caacggcaag catggttcgg aaatcatcga tagctatcgt tctgtcaccg gtgctgcgga
     2641 actgctggag cagctcgtaa atgcggatgc tgtaggtgct ggaaaggctg gagacgttct
     2701 tcacgactac ccgcgtaaac tgccccttga gctgtgtcag atacggcatg atggcatcgg
     2761 agaaggtgat atctactttc gcctcacctt tgaaatactg cgcccggtac tgcacccagc
     2821 ggaactcttc tcgtttttcc tcgttcttga tgattactga cctgtcccag aggcgatcta
     2881 tcgcttcctt gagcctagct tcagcgtgct gccgtcccat gtcaggaaag gcatcatgaa
     2941 actctgcagc cgtgatggtc atggtttttt gttcatccgg cgaaggggaa tcttctccgg
     3001 atttcaggcg gctgatacag accagcagga agcgctgttc ctgcaaagtg agcttgtagc
     3061 ttgcctcgat aagggcgttt gatttgtaag ctatcagatc tgacatttcg cttgctcgtt
     3121 gctattggtg gcgttttttt actgtacgcc acttatcata tgggtggaag tttttttttt
     3181 gcaaccccct ttttctgtgg attacacccc caaaagctcc acctttcacc cccaaaagct
     3241 ccacctttca cccccaaaag ctccaccttt cacccccaaa agctccactt atcttgccag
     3301 aacatcttta aaatcatggt gttacaagac ctaaaaaggt tttaaaaaaa agatctaaaa
     3361 ataagatctt aaaaacttaa cggcgagcct gtggataact tggggtgctg aaaacaaaaa
     3421 accaataaaa acaatgcgtt gcaataaagc agaattgcaa gtttcgaata caccccctcc
     3481 aaccctgtgg aaacccagta ttcatgcggg ttcagcgcat atcacctgcc gaaaaaccgt
     3541 gcgtatgtgc tcaacctggc gactgattca cttccgcttc ctcgcccact cgctcgctaa
     3601 cgctcggtcg ctggggctgc ggcgagcgga cgatacaatc actgttctgg cagcgcctcc
     3661 agctcgttaa tcagcggcag tatgcgcgtc acatcagcct gtcgtgtcac ctctgccttc
     3721 aggaactgga gtaaccaggc acgcaactgc acctcctccg gctttctgac ctttgccagc
     3781 aatgcagcac ccacaacaac cttctgcgct gtgtccaggc tccggctctt cgccttcagg
     3841 cgctgtaatc tcgcctcagc atcagcaatc ttctgttcga gcgttctgct catgatgtaa
     3901 ctccgtgcgc gatgaaaaat cgcattttag cgcgtcactg gtagtttaaa aactgagctg
     3961 gcataatgca cggcaaatca cgaagtgcgc acttatacaa tctccactcc gtttcgattg
     4021 tgtgcgccct gcggggctga aagaaaacta caaaaaggca ttacggcaga a
//
