LOCUS       NZ_OZ039050             4087 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30224_1#201 plasmid 6, complete sequence.
ACCESSION   NZ_OZ039050
VERSION     NZ_OZ039050.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068025
            Assembly: GCF_964040665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964040665.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:48:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,099
            CDSs (total)                      :: 4,986
            Genes (coding)                    :: 4,792
            CDSs (with protein)               :: 4,792
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 68 of 194
            Pseudo Genes (incomplete)         :: 143 of 194
            Pseudo Genes (internal stop)      :: 35 of 194
            Pseudo Genes (multiple problems)  :: 44 of 194
            Pseudo Genes (short protein)      :: 1 of 194
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ039050.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30224_1#201"
                     /db_xref="taxon:562"
                     /plasmid="6"
     gene            88..2247
                     /gene="mobQ"
                     /locus_tag="ABGU55_RS25465"
     CDS             88..2247
                     /gene="mobQ"
                     /locus_tag="ABGU55_RS25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_023142279.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTEYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLATL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2503..3210)
                     /locus_tag="ABGU55_RS25470"
     CDS             complement(2503..3210)
                     /locus_tag="ABGU55_RS25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3742..3996)
                     /gene="mobC"
                     /locus_tag="ABGU55_RS25475"
     CDS             complement(3742..3996)
                     /gene="mobC"
                     /locus_tag="ABGU55_RS25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2..87)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.17e-37"
     Typing          88..446
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3998..4087)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3e-39"
     Typing          complement(2504..3210)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..2244
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.599"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcgcactt atacaatctc cactccgttt cgattgtgtg cgccctgcgg ggctgaaaga
       61 aaaccacaaa aaggcattac ggcagaaatg gcgatttatc atctcagcat gaaaatcatc
      121 tccagaaaca gcggctacag cgccgttgct tctgctgcct atcgttccgg ttcactgatg
      181 cttgatgagc gtaccggact gactcacgac tacacccgca aaagcggtgt ggctgaagct
      241 gtgattctga ctcccgcgac agcccctgcc tggtgtacga accgtgccga actctggaat
      301 gccgttgaga aggccgaacg ccggaagaac tcacaacttg cgcgggaaat tgaactcgcc
      361 atcccccgcg aactaccgca ggatgccgca cgggaaaccg ttctcgcctt tgtcagagaa
      421 aactttgtca gtcagggcat gattgctgat gtggcgttcc atcacatgga taaaacaaat
      481 ccccatgcgc acatcatgct gaccacgaga gctgtcggac ctgctggatt cggtggaaag
      541 gtcagggact ggaacgaccg gacacatgcc gagacgtggc gtgcatcatg ggctgaccat
      601 gcgaacaggg cactggcgaa cgccggttat caggaagaga tagaccatcg ttcatacgag
      661 cgacaggggc ttgagaaaac gcctggtatt cacctcggaa agtctgcctg tgcgatggaa
      721 acacgcggta tcgaaacaga acgcggcgaa cagaatcgtc tgattaacag acttaacctg
      781 gaaatacaga tttcccgtac cgaactcaga aacagcgggc tgacagagcc tgcccgccgt
      841 gtggctgacc ttctgaatat cgtgcttcct gacaacgcaa cttcgtacac actacgtgac
      901 atcatcgaag cgttgccgaa ggacagtaat gcggcatgga aactcaccag taatgccatg
      961 tcgatgatgg cagatatgca ggcgacgcgc agacgctggg aagagctgaa caccgaacga
     1021 aaggaagcga tgagccatgc tgccagcctg aaaaaatcat cgccgttttc gtccggtttt
     1081 tcccgtattc ccctgatgca atgggcggca ccggaatacc gtagcgaaca gggaaaaatc
     1141 cagtcgctga gccagcaaat ggaaaaattg cgacagcatt accggaaggt ggagaaacag
     1201 gacattcccg cgagtcagac ggcatttgaa aaacagtggc agcagtgggg ggcatccggc
     1261 cttgcagctc tcagagtcca gttgcttaaa cgtgaagagg aattgcgccg gaaggagcag
     1321 gaggatacag aacgtagtcg cgcggagcga cgagcggaac agctcaggaa aaatgacaat
     1381 gccgttatgg attcaggggt actgagcgca gtcgttacgg agtatggcga ggcccccatg
     1441 cctggtaacg gggaaatgac ctgttacctt ctgcttcaca accggaacgg agaatacacg
     1501 ctctggggga acgagctgga gaaataccgc accagaatct ttgagcgtgt tgatttaatg
     1561 cgtgacagga gtggttatat ctgtgaccga agcgaaatcg gtcaacatcc accactgcaa
     1621 cgtgtttatt cctctgcgac ctttgagcaa cttctgacgc aggtttgcca gacatggccg
     1681 caatataccc gcaatctccg ccagccgaag acatggccgg agtctttctg tcttggagaa
     1741 gaccgccagc cagccatgcc atcactggca gcaaggaagg tggattttac gcagggacgt
     1801 ctgctagcga cgctgatgcc ggtaatgagt tctgtcgacc gggaaacccg ccagcttcag
     1861 cttttgctgg tcatgggcgt ggatgactct ctgggcggtg ttgtccggct taatggtacg
     1921 ctgtatcccg cgtttgccgt tccctcagcc gataacagcc agctggtcat cagtgcactg
     1981 accgataagg ggttacggta tgcagggtat ggtgttgccg taaatcacga tgcagacagc
     2041 cacatatcac ctgcgccgga acttatggag tttcatctga aaacccgtga ggcaccgctt
     2101 tttgctgccg tcaacacacc agaaaaacag ccggatcatc tgttccgctc gctgggtttt
     2161 aatcgtacct gggacgaatg gcggcgcgag gaagatgcac gaacacacgc caccgagagg
     2221 agacatgatc gcggttggtc acagtgaatg tgaacaatat tttgttttgt ttaacatcgt
     2281 gttatgttcg tcgatgttct tcgatgttca tcgacaggga tgaatgcaag aagcacacac
     2341 ataatgcaag catagttttg tttttccgcc cccgaaaaat gggggttctt ttttcgctgc
     2401 attagagcgc ttttgacgca atttttcact tcaatagtac cttcgcacta ctaacacaaa
     2461 aaaaacgcag cagggagcgt tttaggtgcc ttaaaatcga ttctagatcg tcatcttgat
     2521 ctgtttgtct tccttgaagc gaaaatgcag ggctacaacc gtgcgcccct ttttgatcgt
     2581 ctcaaccgtt acagccaggt cgcttttttt gttcagttcg tcaacacagg gttttattag
     2641 atccctgttt aacgttttga aatctttata tttaccatca agcatcagtg atgatttaaa
     2701 atcatcgagg gcgataatac gttccccagt actgcggaat tgctggagaa tttcgtatat
     2761 tcttatgctg taacttcttg ataaattaga aatattttta atgacgactc tggtgaattg
     2821 ccccttcaac tgtgtcagat aaggcattac cgcgtcagaa aaagtgattt gtgctttccc
     2881 ctctcccctc gcgtactgtg cccgatactg aatccagcgg aactcctcgc gcttctcgtc
     2941 gtccttgaga atgatcgacc tgtcccacaa acgatcgatc gcttcctgta gctgaacctc
     3001 ggcatttttc cgccccatat cagggaatga atcaaaatat tccgcagctg taatggtcat
     3061 tgtcttttgc aattcaggtg aagctacgtc agtaccggag ttaagtcggc ttatgcagag
     3121 caataagaaa cgctgttctt gtaaagttag cttgtaactg gcctcaacca gcgcattgga
     3181 cttataggct atcagagggt ttgatgtcat tttaaccacc aggccaaaaa tgaggtgaaa
     3241 taaaaaccta tcacctttag cattggtgag caattatatt ttcacccccc attttcctgt
     3301 gaatattacc ttaaatctcc tcacctttac cttaaattac ctcacccaag caccttaaat
     3361 tatatcacct ttaccttaaa tcatatcacc tatagcgttg taacattttg ttttctataa
     3421 ggaattttgg cgctaaaaca agtattaaaa acaagatctt aaaaacaaga tctaaaaact
     3481 taacgacgag cctgtggata acttggagtg ctgaaaacaa aaaaccaata aaaacaatgc
     3541 gttgcaaaaa agcagaattg caagttccga atacaccccc tccaaccctg tggaaatcca
     3601 gtattcatgc gggttcagcg catatcacct cccgaaaaac cgtgcgtatg tgctcaatca
     3661 ggcgacggat tcacttccgc ttcctcgccc actcgctcgc taacgctcgg tcgctgggac
     3721 tgcggcgagc ggacgataca atcactgtcc tggcagcgct tccagctcgt taatcagcgg
     3781 cagtatgcgc gtcacatcag cctgtcgtgt cacctctgcc ttcaggaact ggagtaacca
     3841 ggcacgcaac tgcacctcct ccggctttct gacctttgcc agcaatgctg cacccacaat
     3901 gaccttctgc gctgtgtcca gactcctgct cttcgccttc aggcgctgta atctggcctc
     3961 agcatcagca atcttctgtt cgagtgttct gctcatgatg taactccgtg cgcgatgaaa
     4021 aatcgcattt tagcgcgtca ctggtagttt aaaaactgag ctggcataat gcacggaaaa
     4081 tcacgaa
//
