LOCUS       NZ_OZ039049             5159 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30224_1#201 plasmid 5, complete sequence.
ACCESSION   NZ_OZ039049
VERSION     NZ_OZ039049.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068025
            Assembly: GCF_964040665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964040665.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:48:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,099
            CDSs (total)                      :: 4,986
            Genes (coding)                    :: 4,792
            CDSs (with protein)               :: 4,792
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 68 of 194
            Pseudo Genes (incomplete)         :: 143 of 194
            Pseudo Genes (internal stop)      :: 35 of 194
            Pseudo Genes (multiple problems)  :: 44 of 194
            Pseudo Genes (short protein)      :: 1 of 194
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ039049.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30224_1#201"
                     /db_xref="taxon:562"
                     /plasmid="5"
     gene            52..438
                     /locus_tag="ABGU55_RS25430"
     CDS             52..438
                     /locus_tag="ABGU55_RS25430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050502159.1"
                     /translation="MTGRSRSQFYRDMRSGLVSYRTSQDGRREFETSELIRAYGELKRN
                     ETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQAQDTERRRQEAERKQLQN
                     EIAQLRQALEQEKKRGFWSRLSGH"
                     /name="hypothetical protein"
     gene            519..938
                     /locus_tag="ABGU55_RS25435"
     CDS             519..938
                     /locus_tag="ABGU55_RS25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1163..1486)
                     /gene="mobA"
                     /locus_tag="ABGU55_RS25440"
     CDS             complement(1163..1486)
                     /gene="mobA"
                     /locus_tag="ABGU55_RS25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            1770..1964
                     /locus_tag="ABGU55_RS25445"
     CDS             1770..1964
                     /locus_tag="ABGU55_RS25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041621.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2806..2943)
                     /locus_tag="ABGU55_RS25450"
     CDS             complement(2806..2943)
                     /locus_tag="ABGU55_RS25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001329514.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            2991..3332
                     /locus_tag="ABGU55_RS25455"
     CDS             2991..3332
                     /locus_tag="ABGU55_RS25455"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058755.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            complement(3329..4900)
                     /locus_tag="ABGU55_RS25460"
     CDS             complement(3329..4900)
                     /locus_tag="ABGU55_RS25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_346673934.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGSGGGGGTGGS
                     KSESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEAL
                     RHNSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEK
                     EQAETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSS
                     SIHARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARA
                     GDEMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSCIHDAEDNLKTAQTN
                     LLNSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKD
                     VLNKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDT
                     GDWKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTI
                     NEVLGI"
                     /name="colicin-like pore-forming protein"
     Typing          complement(1496..1735)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          1994..2523
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.59"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(1167..1486)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.11e-62"
ORIGIN
        1 atgggacact gtaactttat ggcatggcac acactccggg acgcactgaa aatgactgga
       61 agatcgcgtt cacagtttta ccgtgatatg cgctcaggtc ttgtcagtta ccgtaccagc
      121 caggacggac gacgggagtt tgaaaccagt gaactgatcc gggcatacgg cgaattaaag
      181 cggaatgaga caccagaaag gcacactcaa ggacactcag aaaaacctca tgatcagcag
      241 acagaacgca ttctccggga actgaatgag ctgaaacaat gcctgacgct gatgcttgag
      301 gataaacaag cccaggatac ggagcgccgg cgccaggaag cagaacggaa acagctacag
      361 aatgagatag cccagctcag gcaggctctg gagcaggaaa agaaacgggg attctggtcc
      421 aggctgtccg gtcactgaac gcgctcagaa actgatgata agataatctt cggataataa
      481 ctcaccgaga ataaatactt taaggtaggg agacactcat gagacgtacc ggaaacaaac
      541 tttgtcttat cgccatgata acagcaacag tagctctcac agcctgtacc ccaaagggca
      601 gcgtggaaca acatacccgg cattacgtat atgcttctga tgacggtttt gatcccaact
      661 tttccaccca aaaagccgac acaacacgaa tgatggtgcc tttttttcgg cagttctggg
      721 atatgggagc taaagataaa gcgacaggaa aatcacggag tgatgtgcaa caacgcattc
      781 aacagtttca cagccaggaa tttttaaact cactccgggg cacaactcaa tttgcgggta
      841 ctgattaccg cagcaaagac cttaccccga aaaaatccag gctgctggct gacacgattt
      901 ctgcggttta tctcgatggc tacgagggca gacagtaagt ggatttacca taatccctta
      961 attgtacgca ccactgaaac gcgctgaacg cgattctgac gcagacaccg caaaaagaga
     1021 tgttttaccc cggaaaaaca ggaaggatgc gtcaggatcg ttttcagctc tctgcatagc
     1081 tatgcatgaa agcgaatggt gatcactttg ggagcttacg gtgttcatac cgtcagtttt
     1141 cgacagtttt ctctccggga agctaatctg ccataagcct ggataacagg gcacggtgat
     1201 actccgtaat agcgatcagc acctccgcat actccctgtc cccgacacgc ttgccgtcga
     1261 taaagagttt tttctgtaac gcccccagtc tcataacttc tttcagtacc cgatcatcag
     1321 tcagtgagtt aactttctta ccgagagctg ccgcccgtaa aaaaccggaa acggtcttgc
     1381 cgcattcagc agcccttttt ctgatttcct ggtcttcttc tgccgtcaga cgaacagggc
     1441 gactgatagc ccgcctgcgc gtattacttc cacttcgttt tgtcataaaa catggctccg
     1501 tatctgacat gggtgtcggg gcaaagccct gaccagggta attgtaatag cgtgcatgta
     1561 tgcgcggtat aacaattaca catcctgtcc tgtcagcaag ttcgaattac cgacaaaggc
     1621 acggcagttt tttggcagtg acagtattgt caggcagaaa tacccgtcaa agcgtcagac
     1681 ggcagaatac cggcggtaaa acagtcgaaa agtggtacgc gatataatca cacaacgcag
     1741 atgacagaca gaacggagaa aacgaaaaga tgaacaagca gcagcaagcc gtactcaaca
     1801 tggcaggatt cataaaaagc caaagcctga cgctgctcga aaagctggac gcactcgatg
     1861 ctgacgaaca ggctaccatg tgtgagaagc tgcacgaact cgcagaagaa ctccagaaca
     1921 gcatccagac acgctttgaa gtagaaaata gcacagagag ataacgctca ggacaggatg
     1981 ccgaacggca caacagagcg taaggagaaa atgtcacata aggcgctctt ccgcttcctc
     2041 gctcactgac tcgctacgct cggacgttcg actgcggcga gcggtgtcag ctcactcaaa
     2101 agcggtaata cggttatcca cagaatcagg ggataaagcc ggaaagaaca tgtgagcaaa
     2161 aagcaaagca ccggaagaag ccagcgccgc aggcgttttt ccataggctc cgcccccctg
     2221 acgagcatca caaaaatcga cgctcaagcc agaggtggcg aaacccgaca ggactataaa
     2281 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2341 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2401 gctgttggta tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2461 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2521 taagacacga cttagcgcca ctggcagcag ccgttggtaa cttggttagc agagcgatga
     2581 tggcgcaaat ggtgcaacag agttcttgaa gtagtggccc gactacggct acactggaag
     2641 gacagtattt ggtatctgcg ctctgctgaa gccagttacc tcggttaagc agttccccaa
     2701 ctgacttaac cttcgatcaa accgcctccc caggcggttt tttcgtttac aggcagcaga
     2761 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact gaacagagat
     2821 ccccgtcaat ttagaagaag atgatggagc cacagtccct ccctgaacat cacgtatata
     2881 gttagcctga catccaacaa ggaggtttat agcgaatatt cccacaaaaa atcttttcct
     2941 cataactcga tccttataaa atgaaaagaa tatataacga ggtttaattt atgagtttaa
     3001 gatactacat aaaaaatatt ttgtttggcc tatactgcgc acttatatat atatacctta
     3061 taacaaaaaa caacgaaggg tattatttcc tagcgtcaga taagatgcta tacgcaatag
     3121 tgataagcac tattctatgc ccatattcaa aatatgctat tgaacacata ttttttaagt
     3181 tcataaagaa agattttttc agaaaaagaa aaaacctaaa taatgccccc gtggcaaaat
     3241 taaacctatt tatgctatac aatctacttt gtttggtcct agcaatccca tttggattgc
     3301 taggactgtt tatatcaata aagaataatt aaatacccaa cacctcattt atagtattaa
     3361 gtttattctt atcaataaag gcacatagaa tgcctgtaac aatagcaatc ccccagatac
     3421 ctaatgtagt tccagcaagc acactaaaaa gtaaaacaac aacataacta actccagcat
     3481 ctacagcttt cttctctaat gtaagaaata gcggcttcca gtcacctgta tccttaattt
     3541 ttaggatatc agataccaca tcatatccaa aagaaacatg tcccgtaatc ttcaagtact
     3601 tggcaaactg atctaaatgc ttagcccagt cttcatactt aaccgcctcc agtgcattga
     3661 aaattgcatc acggtctgct ttgctgaatt tcttatttaa tacatccttg tatttctcaa
     3721 aagcagctag agcttcattc acattgctga ttttcttacc tttagattta tcagcaagtt
     3781 cctgtgccat ttttgaatat ttttcaccat atttttcaga tagcgtttga taaaagctaa
     3841 ctgttgcatc cacagcatcc ttaatctgcg agttcaggag attagtctgt gctgttttca
     3901 aattatcttc agcatcatga atacatgaaa ctgctgtact tcgcttatta ttagcctgag
     3961 aaatagcctc ttggattcca tttatctcag agctaatctg gttaagacgc gtttctgatg
     4021 ctgttacctg cttttgtttt tcctccatct catcaccagc tctcgcccgt agtctggtgg
     4081 cctcaaaaaa aggacgactc tgaagcggat cattcgccct aggagataaa agtttaaccc
     4141 tttcatccag ttctttatat ttagcagatg cctgatccaa ctcatttcgt tttccggaca
     4201 gcgtattcgt ttctgcatca cgggcatgaa tactggagct taaacgggta ttgagcgtat
     4261 taatctcttc atccactttc gccagctcag attgtgcagc agcaagattt ttttgtgcca
     4321 cctccacagc ccgggcctct tcactcaatg ctgccaggcg tttctcttca tcctcagcca
     4381 gtttcaattg gcgttctgtt tcagcctgct ccttctctat ctctttacgt cgttgttctg
     4441 cttcctgaaa agccttttcc gcagcttccg cttctttacg ggctttttct tctgcttttg
     4501 caaggcgcaa ccgctctgct tctgcctgca tcgctgcatt attggcatga gccaggtcaa
     4561 taacctccgg atgagtggaa ttatggcgaa gcgcctcatt cacaatatcc ttcagatgtt
     4621 gagtcagcgc atcccggttt gcttttgctt tagcctgtgc ttctgcggca gcttttgctc
     4681 gggcagcctg ttctgcctgc gttttcttca attgagcagt agaccattta gctgtggcat
     4741 gaatggctgc agaactttca cttttgctac ctccagttcc accaccaccg ccagagccag
     4801 agccactccc gtctggatta ccattcaaaa gagtaatgat tacctgcccc ttatcatcat
     4861 aaggaacacc atctttatag tacgctacag ctgtttccat tataaaatcc tctttgacat
     4921 taaaaacaaa tagttaaaag caatatactg tatatataat cactggtttt atatacagtt
     4981 taaaattacg ccgctgcatt ttccctgtca accctgtgga ttttcatttt tgtgaaaacg
     5041 atcaaaaaaa cagccaccca caccacgtgc tatttccacc cgatgccaca aaaaccagca
     5101 caaacattac cgttctcaga cctcattatg ttttactgaa accatgagat gagacacct
//
