LOCUS       NZ_OZ039047             5631 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30224_1#201 plasmid 3, complete sequence.
ACCESSION   NZ_OZ039047
VERSION     NZ_OZ039047.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068025
            Assembly: GCF_964040665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964040665.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:48:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,099
            CDSs (total)                      :: 4,986
            Genes (coding)                    :: 4,792
            CDSs (with protein)               :: 4,792
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 68 of 194
            Pseudo Genes (incomplete)         :: 143 of 194
            Pseudo Genes (internal stop)      :: 35 of 194
            Pseudo Genes (multiple problems)  :: 44 of 194
            Pseudo Genes (short protein)      :: 1 of 194
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ039047.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30224_1#201"
                     /db_xref="taxon:562"
                     /plasmid="3"
     gene            34..357
                     /locus_tag="ABGU55_RS25370"
     CDS             34..357
                     /locus_tag="ABGU55_RS25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            566..>997
                     /locus_tag="ABGU55_RS25375"
                     /pseudo=""
     CDS             566..>997
                     /locus_tag="ABGU55_RS25375"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1035..1565
                     /locus_tag="ABGU55_RS25380"
     CDS             1035..1565
                     /locus_tag="ABGU55_RS25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            1572..1805
                     /locus_tag="ABGU55_RS25385"
     CDS             1572..1805
                     /locus_tag="ABGU55_RS25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2286..3338
                     /locus_tag="ABGU55_RS25390"
     CDS             2286..3338
                     /locus_tag="ABGU55_RS25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            3340..4206
                     /locus_tag="ABGU55_RS25395"
     CDS             3340..4206
                     /locus_tag="ABGU55_RS25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(5409..5600)
                     /locus_tag="ABGU55_RS25400"
     CDS             complement(5409..5600)
                     /locus_tag="ABGU55_RS25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          5234..5315
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          4590..4777
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          348..1909
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgggggtaa tgataccgat gaaacgcgag aggatgctca cgatacgggt tactgatgat
       61 gaacatgccc ggttactgga acgctgtgag ggtaaacaac tggcggtatg gatgcggcgg
      121 gtctgcctgg gtgagccggt tgcccgttcc gggaagctgc cgacactggc accgccgtta
      181 ctgcgtcagc tggccgccat cgggaataac cttaaccaga cagcgaggaa ggtgaacagc
      241 gggcagtggt cttccggtga ccgggttcag gtggtggccg cactgatggc catcggggat
      301 gagctgcgcc ggctgcgtct ggcggtcagg gaacaggggg cgcgggatga tagttaagtt
      361 tcatgccagg ggaaaaggtg gtggcagtgg tccggttgat tacctgctgg gaagggagcg
      421 taaccgggaa ggggcaacgg tgcttcaggg gaatccggaa gaggtccggg aactcatcga
      481 tgccacgcca tttgcgaaga aatacacgtc aggtgttctg tcgttcgcgg agaaggagct
      541 gccgcctgga gggcgtgaaa aagtgatggc gagctttgag cgtgtactga tgcccggtct
      601 cgaaaagaat cagtacagca tcctgtgggt ggagcaccag gacaagggac ggcttgagct
      661 gaattttgtc attccgaaca tggagctaca gaccggaaaa cgcctccagc cgtactacga
      721 ccgggcagac aggccgagaa ttgatgcctg gcagacgctg gtgaatcacc attacggtct
      781 gcatgacccc aacgccccgg agaaccgccg gacgctgacc cttcctgata acctgccgga
      841 aacgaaacag gcgcttgctg agggcgtcac acgcgggata gacgcgcttt accatgccgg
      901 agagataaaa ggccgtcagg atgtgattca ggcgctcaca gaggcggggc ttgaagtggt
      961 cagggtgacg cgaagcagta tcagcattgc agacccgaac ggcgggagga atatcagact
     1021 gaaaggagcg ttttatgagc aatcttttac agacgggcgc ggagttcgag aaaaagctga
     1081 aagagagagc cgaatctaca gagaaaatgc tgaacgacga gttcaggaag ctcgaagaat
     1141 ctgtaaacag ggaactgacc tcaaacgaat ctttaatcag gaacgctatc aacgaccata
     1201 caacagccct gaaagaactg ctggagaggt atcagaaaac gaccgtcgat acgatggacg
     1261 cgcactggaa aaccgtactg aaaatgagcg tgaaacgctg gctgtggctg attatcgtct
     1321 cagtactgat gtttgccacg accgggagtc tgctgtggta ccagggcatg aagataaacg
     1381 cgaatatgaa tattctccgc gagcagaaag agagtctgga gaagctgaac gcgaagacat
     1441 ggggcgtgcg ttatcacgag gacagcaacg gacgttttct ggtgttgccg aaggggatga
     1501 aagccgaaac gaactggacg aaggacaacg gcaaactgaa cgcagtgaga ctggtgcagg
     1561 agtgacagag catgacggag ctggaaagac atttgctgaa cgcattagag caactgcaac
     1621 aggactatat gcagcggctg aacgaatggg agaacgcctt cgtggaattg cagaaaatgt
     1681 tttcgcttac gcaacgggac aacgcgatgc tgaacgggcg agtcatgcag ttgagtcagc
     1741 aggtgcagca cttgagcgag cggacagaac gcttgagcca gttatacagc gagaacagga
     1801 gataagggat gaacggctga tgcaggaacg cgaacatgag ttatctctgg agcgggagcg
     1861 tcagccggaa atccaggagc ggacactgga tggcccttcg ctgggatggt gaaaccatga
     1921 aaaatgacgt gttcagtgga ttaaggcggg ctaatgtggc ctgtagcatc tggttgcata
     1981 ggtattcatg cggttaaaat ttatcaggtg cgatcgcggc agtttttcgg gtggtttgtt
     2041 gccattttta cctgtctgct gccgtgatcg caccgaacgc gttttggcgg tgcgtacaat
     2101 gcgacgttat ggtaaatgga gcggattttc aggaaggagt gatactaaca actcgaacac
     2161 tgtgcaaata atgatttttt taaaatatcg gtaacgataa taaattattt cgttttacgc
     2221 cggtaataaa tataatgtag tttcagtata taaaaaaata tatgaggtgt cttgtgggag
     2281 ctttcatgaa tcttcaacaa cgtataaata aactaccaca attatcatcc agtttttcat
     2341 ttggaaaaga tattgataat attcattcat ttatctttaa cgaaacttct aaagataaaa
     2401 ttgaagattt actaagaaaa tgggtgtcag gcaatcagcc ctgtgtattt ggtaaattgg
     2461 caagcaagaa aattaaaggg cttgatttcc atttatctat tgtaaatagc ccccaactat
     2521 ataatgatga tggtcatttg tttgattttt tgcgtaatga aagagttcgt tttaaagaac
     2581 gcgcaagaag aggagaggtc agtgcgcatc tcatttattt tattcatcca caacttgcct
     2641 ttgctcgtcc atcagaagag ttagtggaca tacaaaaata tatttgttcg ctgcatatgc
     2701 cagagtgcta tccaataaaa gaggatgtta tttatactga aagcgtacca tttcaggata
     2761 aagatgggct aaaaatatat aaggctggtg ttaatgtttt ttactcttct gctcatagaa
     2821 caagaaatca tgatagaaga ataccagggg gaatacttat ttctgtaaat gccccggggc
     2881 attttatgag gctggcgata gaaaaaggtt tctataaaga tcaagaacaa gcgttagcgg
     2941 atattcgtaa catgactatt cagtctgttg gtaagggtgg gtattcacat ccggaaggga
     3001 tttctacaac atggcattca gaatctaatt tggatcgttt tggatgccca gtgcacaccg
     3061 gtaatagttc ttattacagc ggtttttatc atactgatgt attaattcca ggagaattaa
     3121 ctaaggatga aagattgctc catgaaatag aaaatagtga tccaatgatc ttcaactgga
     3181 atgtattgtt ttatgtttca ttagaagagt ttcctataga tgatccttat tatggagagt
     3241 ttattggtat tcctgtagat gatgcatcta tgttttttaa ctcctttcaa ccaaggaaat
     3301 ttgaaaacaa acccctttat gagaaagagg atgattaata tgattaaagg aatgcttttt
     3361 gcttttttag cagcgttctc atggggggca gctatcgtaa tgagcaaaaa aggagttgaa
     3421 gaactagatg ccggaggggt gtttttctgg cagatttgta gtgcagtaat actatcttgg
     3481 atagttcttt ttataaaaag aaaaaaactc ccattaaact caaaagcgat acttgcttat
     3541 gcgacaggcg tttttgaacc attcttagcg tatactttta cactctacgg actttatttt
     3601 atttcagcag gaagcgcatc aattattttt tcacttgaat cagtgtttat attggtgttg
     3661 tctgttctta tttgctcagt taaaattaat tccccgcaat attttatatt gttccttata
     3721 ggtgcaattg ttggcagtat tttggtagcc ttgccagaca tcagtaatgg atcgaataat
     3781 attgttggat attgtcttgt tgttgccggt gtgttatcgg cagcatttta tgttgttata
     3841 tcgtctcgcc ttattaaatc gtttgagcct atcacattat tgagcggaca gttgacattc
     3901 tcccttatac tgtcatcgct ctttattttt atatctaaat catcaattta tcttccgttg
     3961 gaatccgtga ttattgtttt ttcatctgga atattacaat attttttagc tttttgtttt
     4021 tacttgaact ccttgcgttg gctcccagtt catttggcag ggataatgtt gtactttatt
     4081 ccgctagttg cattattttt gtcatggttg tttttggatg aacaattcac attcttacag
     4141 ggggtaggat ttattgtaac tgtgctaagt gtatattttc ttaacagaaa atactcagag
     4201 gagtaacatt ctgttcagga ttgccccccg gcctccatcg ataccgtgcc aaatgcacgt
     4261 aaaaaacggg agcgtttctg gtgtcggaat gtaaccaagg gggtcagagg cgcgctaaaa
     4321 cgctctcagg ggagtcaaat agagacgttt gtaacaaaaa gatcaaagga tcctcttgag
     4381 atcctttttt tctgcgcgta atctgctgcc tgtaaacgaa aaaaccgcct tgcagggcgg
     4441 tttgatcgaa ggttaagtca gcttgcagac tgcttaacca ggtaactgtc ttatgcgaga
     4501 cgagtcacca aaattgttct tctagtgtag ccgcactccg gcagccactt caagtactct
     4561 ctaaatatct cgcacatgac ttttaccaat ggctgctgcc agtggcgctt tgtggtgtct
     4621 tttccgggtt ggacccaaga gaacagttac cggagaaggc gcagcagtcg ggctgaacgg
     4681 ggggttcctg catacagccc agcccggagc gaactgccta agtcgccccg agataccaga
     4741 agacgtgagc tatgagaaag cgccacgtgt cccgtaaggg agaaaggcgg aaaaggtatc
     4801 cggtaatcga tatggcggga acgtgggggc gcaggaggga gcgattcacc ggaaacggtg
     4861 atgatcttta agccacgtcg ggtttcgcca ccgctggcgg atttcatggt tgagccgcaa
     4921 ggctcccaca gatgcaccga aaaagtgtct gttttgtgaa gtccgtcagg ggggcggagc
     4981 ctatggaaaa agcgcctccg gcgcggcctt tcccggggtt tcgttttttg cgcacatgtt
     5041 ctttcctggt ttatccccgg gttctgtgga taacagggtt agcgtttttg agaaaggaga
     5101 gtccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
     5161 gcgcctgaac tggcattttc tccttacgca tctgtgcggc atttcacacc ggacatggcg
     5221 cacttttcat acaatccgca ctgatgccga atggttaagc cagtatacac tccgctatcg
     5281 ctacgtgact gggtcagggc ttcgccccga caccccaaag gtcaacggcc tgacgcgccc
     5341 tggcgggctt gtctgctccc ggcatccgct cacagacaag ctgtgaccgt ctccgggagc
     5401 tgcatgtgtc agcggttttc accgtcatcc ccgaagcgtg cgaggcaact gcggtaaagc
     5461 tcatcggcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagttcg
     5521 ttgagcttct ccagcagcgt taatgtctgg cctctgatga agcgggccat gttaagggcg
     5581 gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gggttctgtt c
//
