LOCUS       NZ_OZ038809             4074 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30345_1#335 plasmid 4, complete sequence.
ACCESSION   NZ_OZ038809
VERSION     NZ_OZ038809.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068055
            Assembly: GCF_964038895.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964038895.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:55:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,297
            CDSs (total)                      :: 5,184
            Genes (coding)                    :: 4,970
            CDSs (with protein)               :: 4,970
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 82 of 214
            Pseudo Genes (incomplete)         :: 154 of 214
            Pseudo Genes (internal stop)      :: 42 of 214
            Pseudo Genes (multiple problems)  :: 54 of 214
            Pseudo Genes (short protein)      :: 1 of 214
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ038809.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30345_1#335"
                     /db_xref="taxon:562"
                     /plasmid="4"
     gene            1..2160
                     /gene="mobQ"
                     /locus_tag="ABGU69_RS26460"
     CDS             1..2160
                     /gene="mobQ"
                     /locus_tag="ABGU69_RS26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_346714070.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WEEWQREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2408..3109)
                     /locus_tag="ABGU69_RS26465"
     CDS             complement(2408..3109)
                     /locus_tag="ABGU69_RS26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3642..3896)
                     /gene="mobC"
                     /locus_tag="ABGU69_RS26470"
     CDS             complement(3642..3896)
                     /gene="mobC"
                     /locus_tag="ABGU69_RS26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          2..359
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3898..4074)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          complement(2409..3109)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..2157
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.166"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcgattt atcatctcag catgaaaatt atctccagaa gcagcggcta cagcgccgta
       61 gcttccgctg cctatcgttc cggttcactg atgcttgatg agcgtaccgg actgactcac
      121 gactacaccc gcaaaagcgg tgtggctgag gctgtgattc tgacccccgc caccgcccct
      181 gcctggtgta cgaaccgtgc cgaactctgg aatgccgttg agaaggccga acgccggaag
      241 aactcacaac ttgcgcggga aattgaactc gccatccccc acgaactgcc gcaggatgcc
      301 gcacgggaaa ccgttctcgc ctttgtcaga gaaaactttg tcagtcaggg catgattgct
      361 gatgtggcgt tccatcacat ggataaaaca aatccccatg cgcacatcat gctgaccacg
      421 agggctatcg gacctgctgg gttcagcgga aaggtcaggg actggaacga ccggacacat
      481 gccgagacgt ggcgggcatc atgggctgac catgcaaaca gggcactggc gaacgccggt
      541 tatcaggaag agatagacca tcgttcatac gagcgacagg ggcttgagaa aacgcctggt
      601 attcacctcg gaaagtctgc ctgtgcgatg gaaacacgcg gtatcgaaac agaacgcggc
      661 gaacagaatc gtctgattaa cagacttaac ctggaaatac agatttcccg taccgaactc
      721 agaaacagcg ggctgacaga gcctgcccgc cgtgtggctg accttctgaa tatcgtgctt
      781 cctgacaacg caacttcgta cacactacgt gacatcatcg aagcgttgcc gaaggacagt
      841 aatgcggcat ggaaactcac cagtaatgcc atgtcgatga tggcagatat gcaggcgacg
      901 cgcagacgct gggaagagct gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc
      961 ctgaaaaaat catcgccgtt ttcgtccggt ttttcccgta ttcccctgat gcaatgggcg
     1021 gcaccggaat accgtagcga acagggaaaa atccagtcgc tgagccagca aatggaaaaa
     1081 ttgcgacagc attaccggaa ggtggagaaa caggacattc ccgcgagtca gacggcattt
     1141 gaaaaacagt ggcagcagtg gggggcatcc ggccttgcag ctctcagagt ccagttgctt
     1201 aaacgtgaag aggaattgcg ccggaaggag caggaggata cagaacgtag tcgcgcggag
     1261 cgacgagcgg aacagctcag gaaaaatgac aatgccgtta tggattcagg ggtactgagc
     1321 gcagtcgtta cggggtatgg cgaggccccc atgcctggta acggggaaat gacctgttac
     1381 cttctgcttc acaaccggaa cggagaatac acgctctggg ggaacgagct ggagaaatac
     1441 cgcaccagaa tctttgagcg tgttgattta atgcgtgaca ggagtggtta tatctgtgac
     1501 cgaagcgaaa tcggtcaaca tccaccactg caacgtgttt attcctctgc gacctttgag
     1561 caacttctga cgcaggtttg ccagacatgg ccgcaatata cccgcaatct ccgccagccg
     1621 aagacatggc cggagtcttt ctgtcttgga gaagaccgcc agccagccat gccatcactg
     1681 gcagcaagga aggtggattt tacgcaggga cgtctgctac cgacgctgat gccggtaatg
     1741 agttctgtcg accgggaaac ccgccagctt cagcttttgc tggtcatggg cgtggatgac
     1801 tctctgggcg gtgttgtccg gcttaatggt acgctgtatc ccgcgtttgc cgttccctca
     1861 gccgataaca gccagctggt catcagtgca ctgaccgata aggggttacg gtatgcaggg
     1921 tatggtgttg ccgtaaatca cgatgcagac agccacatat cacctgcgcc ggaacttatg
     1981 gagtttcatc tgaaaacccg tgaggcaccg ctttttgctg ccgtcaacac accagaaaaa
     2041 cagccggatc atctgttccg ctcgctgggt tttaatcgta cctgggagga atggcagcgc
     2101 gaggaagatg cacgaacaca cgccaccgag aggagacatg atcgcggttg gtcacagtga
     2161 atgtgaacaa tattttgttt tgtttaacat cgtgttatgt tcgtcgatgt tcatcgacag
     2221 ggatgaatgc aagaagcaca ctcacataac gcaagcatag ttttgttttt ccgcccccaa
     2281 aaattggggg gtcttttttc gctgcatcag agcgtttttg acgcaatttt tcacttcaat
     2341 agtaccttcg cactactaac acaaaaaaaa cgcagcaggg agcgttttag gtgccttaaa
     2401 atcgatttta gatcgtcatc ttgatctgtt tgtcttcctt gaagcgaaaa tgcagggcta
     2461 caaccgtgcg cccctttttg atcgtctcaa cagttacaac cagatcgctt ttttcgttca
     2521 gttcggcgat ggctggcttg attatcatct tattcaagtc tttgaattgt tgatatttat
     2581 cttcaacggc aagcatggtt cggaaatcat cgatagctat cgttctgtca ccggtgctgc
     2641 ggaactgctg gagcagctcg taaatgcgga tgctgtaggt gctggaaagg ctggagacgt
     2701 tcttcacgac tacccgcgta aactgcccct tgagctgtgt cagatacggc atgatggcat
     2761 cggagaaggt gatatctact ttcgcctcac ctttgaaata ctgcgcccgg tactgcaccc
     2821 agcggaactc ttctcgtttt tcctcgttct tgatgattac tgacctgtcc cagaggcgat
     2881 ctatcgcttc cttgagccta gcttcagcgt gctgccgtcc catgtcagga aaggcatcat
     2941 gaaactctgc agccgtgatg gtcatggttt tttgttcatc cggcgaaggg gaatcttctc
     3001 cggattttag gcggctgata cagaccagca ggaagcgctg ttcctgcaaa gtgagcttgt
     3061 agcttgcctc gataagggcg tttgatttgt aagctatcag atctgacatt tcgcttgctc
     3121 gttgctattg gtggcgtttt tttactgtac gccacttatc atatgggtgg aagttttttt
     3181 tttgcaaccc cctttttctg tggattacac ccccaaaagc tccacctttc acccccaaaa
     3241 gctccacctt tcacccccaa aagctccacc tttcaccccc aaaagctcca cttatcttgc
     3301 cagaacatct ttaaaatcat ggtgttacaa gacctaaaaa ggttttaaaa aaaagatcta
     3361 aaaataagat cttaaaaact taacggcgag cctgtggata acttggggtg ctgaaaacaa
     3421 aaaaccaata aaaacaatgc gttgcaataa agcagaattg caagtttcga atacaccccc
     3481 tccaaccctg tggaaaccca gtattcatgc gggttcagcg catatcacct gccgaaaaac
     3541 cgtgcgtatg tgctcaacct ggcgactgat tcacttccgc ttcctcgccc actcgctcgc
     3601 taacgctcgg tcgctggggc tgcggcgagc ggacgataca atcactgttc tggcagcgcc
     3661 tccagctcgt taatcagcgg cagtatgcgc gtcacatcag cctgtcgtgt cacctctgcc
     3721 ttcaggaact ggagtaacca ggcacgcaac tgcacctcct ccggctttct gacctttgcc
     3781 agcaatgcag cacccacaac aaccttctgc gctgtgtcca ggctccggct cttcgccttc
     3841 aggcgctgta atctcgcctc agcatcagca atcttctgtt cgagcgttct gctcatgatg
     3901 taactccgtg cgcgatgaaa aatcgcattt tagcgcgtca ctggtagttt aaaaactgag
     3961 ctggcataat gcacggcaaa tcacgaagtg cgcacttata caatctccac tccgtttcga
     4021 ttgtgtgcgc cctgcggggc tgaaagaaaa ctacaaaaag gcattacggc agaa
//
