LOCUS       NZ_OZ038808             6078 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30345_1#335 plasmid 3, complete sequence.
ACCESSION   NZ_OZ038808
VERSION     NZ_OZ038808.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068055
            Assembly: GCF_964038895.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964038895.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:55:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,297
            CDSs (total)                      :: 5,184
            Genes (coding)                    :: 4,970
            CDSs (with protein)               :: 4,970
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 82 of 214
            Pseudo Genes (incomplete)         :: 154 of 214
            Pseudo Genes (internal stop)      :: 42 of 214
            Pseudo Genes (multiple problems)  :: 54 of 214
            Pseudo Genes (short protein)      :: 1 of 214
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ038808.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30345_1#335"
                     /db_xref="taxon:562"
                     /plasmid="3"
     gene            1..534
                     /locus_tag="ABGU69_RS26420"
     CDS             1..534
                     /locus_tag="ABGU69_RS26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168772.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            756..2483
                     /locus_tag="ABGU69_RS26425"
     CDS             756..2483
                     /locus_tag="ABGU69_RS26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032490394.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGHGNGGGNGNSGGGSNSSVAAVAFGFPALAAPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPKMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTNLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2486..2746
                     /locus_tag="ABGU69_RS26430"
     CDS             2486..2746
                     /locus_tag="ABGU69_RS26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420692.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            2808..2951
                     /locus_tag="ABGU69_RS26435"
     CDS             2808..2951
                     /locus_tag="ABGU69_RS26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            3040..3249
                     /locus_tag="ABGU69_RS26440"
     CDS             3040..3249
                     /locus_tag="ABGU69_RS26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_346714069.1"
                     /translation="MRAKIPVQNFPPRGRKLVILTCSPFGIVEIDRASLSCIVMQPSKN
                     DSGAFFWFSLVFPCLFAFFAPESD"
                     /name="hypothetical protein"
     gene            3446..4381
                     /locus_tag="ABGU69_RS26445"
     CDS             3446..4381
                     /locus_tag="ABGU69_RS26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271369.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSTSA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(4575..4973)
                     /locus_tag="ABGU69_RS26450"
     CDS             complement(4575..4973)
                     /locus_tag="ABGU69_RS26450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            5150..6076
                     /locus_tag="ABGU69_RS26455"
     CDS             5150..6076
                     /locus_tag="ABGU69_RS26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001314449.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     BGC             1..6078
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3447..4381
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.551"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5151..6073
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggccagca cagaagaaaa attactcacg ctcctgttat cggtggagca tctgcaacat
       61 accgcaatgg agcaacagaa agctgttgct gattctctgg ctgtggcaag ggaaacactt
      121 cgcggatatc gggatatttc tgacggtgtg gcaaaacagg tcagggatga agtcagaaac
      181 gaggtgcaga agctggatgt tgcagggctg ataggtgagc gcatcagcca ggattttcag
      241 cggctggaag agtcagtcgc aaacatgaag gcaaacgcgg atgccctgaa cagggatatt
      301 gcgacaattc acagcaatat cgtgcaggaa tacaacaccc ttaaagggta tagctggaaa
      361 tggctgggtg gggtgtcagt ggcttttctg gttatgttct gtgtgcttgg atggggaatg
      421 aaaacaatgc tcgacaggaa ctacaacgcc acagcggcgg tgtatcagaa agttgtgaca
      481 ctggaagata aactggcgga gaaacctgtc acgggcaaga gccggaaaaa ctgacgcgct
      541 tcgcttgtcc aacgcctcac gacccgcgaa aggatttcgc gttccgttca ccgttttcat
      601 gagtgattcc tccgaactgc caaaaaatat cctccccctg acttgacaga aaaaataatg
      661 gcgagtactt tttgatctgt acataaaacc agtggtttta tgtacagtat taactatgta
      721 attaattgtt ttaacgctta aaagagggaa tttttatgag cggtggagat ggacgcgacc
      781 acaacagtgg cgcacataac acaggtggta acattaatgg cggccctacg gggcttggtg
      841 gaaatggtgg ggcttctgac ggctccggat ggagttcgga aaataaccca tggggtggcg
      901 gttctggtag tggtgttcac tggggaggtg gctccggcca tggcaatggc ggggggaatg
      961 gtaattccgg tggtggcagc aattcatccg tagcagcagt ggcatttggt tttcctgctt
     1021 tggcagctcc tggtgccgga acactgggta tcgccgtctc tggtgaggct ttatctgcgg
     1081 caatagcgga tatctttgca gccctgaaag gtccgtttaa attcagtgca tggggtattg
     1141 cgctttacgg cattctgcca tctgaaatag caaaagatga cccgaaaatg atgtcaaaga
     1201 ttgtgacgtc attaccggca gaaacagtaa caaatgttca ggtcagtaca cttccgctgg
     1261 accaggcaac tgttagcgtg acaaaacgtg tgaccgatgt tgtgaaggac acacgacagc
     1321 atattgccgt agttgcaggt gtacctatga gtgtgcctgt tgtgaatgct aaaccaacac
     1381 gtaccccagg tgtatttcac gcatcatttc ctggtgtccc ttctctgaca gtcagcacgg
     1441 tcaaaggtct tcctgtgtca acaaaccttc cccgtggtat tacggaggat aagggccgga
     1501 ctgccgttcc tgcaggattt acctttggtg gtggttcaca tgaagcggtg atccgtttcc
     1561 cgaaagaaag tgggcagaag ccgatttatg tatcagtgac agatgttctt acccctgcac
     1621 aggtaaaaca acgtcaggat gaggaaaatc gcctccagca ggaatggaat gacacacatc
     1681 ctgtagaagt ggctgaacgc aattatgaac aggcccgcgc agaactggat caggctaata
     1741 aagatgttgc cagaaaccag gaacgacagg ctaaagctgt tcaggtttat aattctcgta
     1801 aaagtgaact tgatgcagcg aataaaactc ttgctgatgc aaaggctgaa ataaaacaat
     1861 tcgagcgatt tgcccgagaa ccaatggctg ctggtcaccg aatgtggcaa atggcagggc
     1921 ttaaggccca gcgggcacag acggatgtaa ataataagaa ggctgcattt gatgctgctg
     1981 caaaagataa gtctgatgct gatgctgcgt taggtgctgc gctggaacgc cgtaagcaga
     2041 aggaaaataa agaaaaggac tctaaggata aattagataa ggagagtaaa cggaataagc
     2101 cagggaaggc gacgggtaaa ggaaaaccag ttggtgataa atggcttgat gatgcgggta
     2161 aagattcagg agctccaatt cctgatcgca ttgctgataa gttgcgtgat aaagaattta
     2221 aaagttttga cgatttccgg aagaaattct gggaagaagt gtcaaaagat cccgagctta
     2281 gtaagcaatt taaagacagt aataagacga acattcaaaa gggaaaagca ccttttgcaa
     2341 ggaagaaaga ccaagtaggt ggtagggaac gctttgaatt acatcatgat aaaccaatca
     2401 gtcaggatgg tggtgtctat gatatggata atatcagagt gaccacacct aagcgacata
     2461 ttgatattca tagaggtaag taaaaatgga actgaaacat agtattagtg attataccga
     2521 ggctgaattt ctggagtttg taaaaaaaat atgtagagct gaaggtgcta ctgaagagga
     2581 tgacaataaa ttagtgagag agtttgagcg attaactgag cacccagatg gttcagatct
     2641 gatttattat cctcgcgatg acagggaaga tagtcctgaa gggattgtta aagaaattaa
     2701 agaatggcga gctgctaacg gtaagccagg atttaaacag ggctaaaata tgaatgccgg
     2761 ttgtttatgg atgaatggct ggcattcttt cacaacaagg agtcgttatg aaaaaaataa
     2821 cagggattat tttattgctt cttgcagtca ttattctggc tgcatgtcag gcaaactata
     2881 tccgggatgt tcagggcggg actgtatccc cgtcatcaac agctgaagtg accggattag
     2941 caacgcagta acccgaaatc ctctttgaca aaaacaaagc gtgtcaggct gattctgatg
     3001 cgcttttttt tgaaatgtca caaaaattcc atgcgggaga tgagagctaa aatccccgtg
     3061 cagaactttc cacccagggg gagaaaactt gtcattttga cctgttcgcc cttcggaata
     3121 gtcgaaatcg atcgcgcatc gctttcgtgc atagttatgc agccctctaa aaacgattct
     3181 ggcgcgtttt tctggtttag cctggtgttt ccttgtcttt ttgcgttttt tgcgccagaa
     3241 agcgattgag ggcgttttac agggtacgta caacgggagt tatggtaaat ggatcgggtt
     3301 ttcgggaagg atcgacagga tttgccgttg ggtgtagtgt aagcgactga aaaacaaacg
     3361 cccctgaaat tcgtgtctct ccgccaagat tgtcacgaga atacaggggc gaggtcggtc
     3421 gataacctag aaggaatcgt aacacatgag cgccgcgctt caatacttcg aagaaaattt
     3481 acccctccgc ccctatcaca cggatgatct cgctttcggc cttcgtatct ccggtaaagg
     3541 gcgcgcgctt cttgcacggt atatccagca gaaccaacct catgcgcagt tctggctggt
     3601 ttttgacgtt gaccgcgagg gggctgcgat tgactggagc gacctgaacg cccccgcacc
     3661 gaacatcacc ataaaaaatc ccgtgaacgg tcacgcccac ctgctctacg cactcaatat
     3721 cgccgtgaga accgcgcctg atgcttcggt taaggcgctg aaatacgccg ccgcgattga
     3781 acgtgcgttg tgtgaaaaac tgggcgcaga tgtaaactac agcggactga tttgcaaaaa
     3841 tccgttccat ctggaatggc aggtgatgga gtggcgtgag gaagcctata ccctcgatga
     3901 actggctgat tatctcgatt tgagcacttc agcgcgccgt agcatcgata aacattacgg
     3961 gatggggcga aactgccatc tgttcgaaat gacgcgcaaa tgggcttaca gggcgattcg
     4021 tcagggctgg ccagcattct cacagtggct tgatgccgtg attcagcgtg tcgaaatgta
     4081 caacgcatcg cttcccgttc cgctttcacc tcctgaatgt cgggctattg gcaagagtat
     4141 tgcgaaatac acgcacagga acttcacggc ggaaactttc gcacagtatg tggctgatac
     4201 gcacacgcca gaaatacagg ccaagagagg caggaaaggt ggcatcgcta aaggcgaagc
     4261 ctacgatgac aagcgtttca tggcgctatg tatgctggag aatggatatt ctcagaaagc
     4321 tattgcggcg atgttggagg tttctactcg aaccattcga aactggaaaa gcggaaaata
     4381 gcctatatca gataacagcg cctttctggc gtttttttga gcagtaggtc ttttgccgtt
     4441 tttccccgtt cagccctgca atggtgctgt tttccgcatg attgtgctgg tagtgctcgc
     4501 cgcagtctca tgaccgagcg tagcgagcga atgagcgagg aagcaccagg gaaccggaac
     4561 acggatggag gcacttacgc gtctgggttt ggggatgtct ccagatcgta cttaatttcc
     4621 ttaagtgcct gtaatatcct gtctttttta tagcatagct cgcctgtttt tgtgtctacc
     4681 agtcctgctt cgatcagtag cccagccgcg agaaagagtc ggtgattgcg ttctcgtcgt
     4741 tgtttttttc cctcgattcg tttttggacc gcctgttttc gttcgctctc ctgtgcctgt
     4801 aagtcgtttt tttccgctgt tgccagtacg tggaggagtt tgcgttcctg ttctgtgagt
     4861 cctgcttcgt tgtcgagcag gtcaataatc tgtctctgtc gtgcggtctg ttctccgagc
     4921 ttcctgatga atccgatccg tttcttcagc cattcgatgt tgctgatgcg catgttcgcg
     4981 gtgctccctg gtgttttttg cggtgtgttg tggtcgtgca gtatatcgct tgcggtcggt
     5041 gggtgcaggg gggtatcttc cgtgctaatt cgtaaaatta gaaggaagcg cacttatatg
     5101 tttctctcct tcgtcgagaa acagtgcgct cctgcggagg gctgaaaccg tggcaaccta
     5161 tcacctcagc gttaaatttg gtggcaaggg gcaggctgca aaccacgccg attacatcga
     5221 gcgcaaagag aaataccgcg accgtcagga tttggaatat tccgcacacg gaaacatgcc
     5281 ggaatgggcg cgagataatc cctcacattt ctggcaggcg gctgaccagt ttgaacgtgc
     5341 gaacggttcg acctatcggg aacttgaaat tgcattaccc cgtgaactga cagcggaaca
     5401 gcgccttgaa ctggtgcagg attttgtacg tcaggaagct ggcgaacggc acgcctggag
     5461 ttttgccatt cacaatccga aagcgagcat tgacggcggc gaacagcctc atgcgcatat
     5521 catgatgagc cagcgtgtga atgatggcat tgaacgtaca ccggaacagt atttccggcg
     5581 ctataacgcc aggtatcccg agcggggagg tgcgaaaaag gattccggaa gcctgactct
     5641 cactcagcaa aaagagcagt tgcgggaatt gcgaaaacgc tgggaggtga agcacaacga
     5701 acatatgaga aagcatggct ttgaaagggg cttcattgac tgccggacgc tgaaagaaca
     5761 gggcattgaa cgacgtcccg aagtgcatct gggttttgaa gctgccggac ggcttgatga
     5821 tgcccagcgt catgacatca tgcaaaaacg gtctgaaccg gactattccc gccatcttga
     5881 acaggcgcga acggtcgggc atgacatcag ggaatggctg gacactccgg ttaagcaaca
     5941 tgaactggtc agaaggctgc aacatgatgt tgcgcgggag catggctttg acatgagaga
     6001 cagagtgagc agacagaacc gaactgaacc tgaacgggtt aaatcaccgt cacgtggatt
     6061 ttctatggga ttctgaac
//
