LOCUS       NZ_OZ038771             4082 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30348_2#44 plasmid 3, complete sequence.
ACCESSION   NZ_OZ038771
VERSION     NZ_OZ038771.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068062
            Assembly: GCF_964038595.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964038595.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 11:55:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,956
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,621
            CDSs (with protein)               :: 4,621
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 93 of 221
            Pseudo Genes (incomplete)         :: 158 of 221
            Pseudo Genes (internal stop)      :: 46 of 221
            Pseudo Genes (multiple problems)  :: 65 of 221
            Pseudo Genes (short protein)      :: 1 of 221
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ038771.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30348_2#44"
                     /db_xref="taxon:562"
                     /plasmid="3"
     gene            1..2160
                     /gene="mobQ"
                     /locus_tag="ABGU70_RS24770"
     CDS             1..2160
                     /gene="mobQ"
                     /locus_tag="ABGU70_RS24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2416..3117)
                     /locus_tag="ABGU70_RS24775"
     CDS             complement(2416..3117)
                     /locus_tag="ABGU70_RS24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3650..3904)
                     /gene="mobC"
                     /locus_tag="ABGU70_RS24780"
     CDS             complement(3650..3904)
                     /gene="mobC"
                     /locus_tag="ABGU70_RS24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          2..359
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3906..4082)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          complement(2417..3117)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..2157
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcgattt atcatctcag catgaaaatc atctccagaa acagcggcta cagcgccgtt
       61 gcttctgctg cctatcgttc cggttcactg atgcttgatg agcgtaccgg actgactcac
      121 gactacaccc gcaaaagcgg tgtggctgaa gctgtgattc tgactcccgc gacagcccct
      181 gcctggtgta cgaaccgtgc cgaactctgg aatgccgttg agaaggccga acgccggaag
      241 aactcacaac ttgcgcggga aattgaactc gccatccccc gcgaactacc gcaggatgcc
      301 gcacgggaaa ccgttctcgc ctttgtcaga gaaaactttg tcagtcaggg catgattgct
      361 gatgtggcgt tccatcacat ggataaaaca aatccccatg cgcacatcat gctgaccacg
      421 agagctgtcg gacctgctgg attcggtgga aaggtcaggg actggaacga ccggacacat
      481 gccgagacgt ggcgtgcatc atgggctgac catgcgaaca gggcactggc gaacgccggt
      541 tatcaggaag agatagacca tcgttcatac gagcgacagg ggcttgagaa aacgcctggt
      601 attcacctcg gaaagtctgc ctgtgcgatg gaaacacgcg gtatcgaaac agaacgcggc
      661 gaacagaatc gtctgattaa cagacttaac ctggaaatac agatttcccg taccgaactc
      721 agaaacagcg ggctgacaga gcctgcccgc cgtgtggctg accttctgaa tatcgtgctt
      781 cctgacaacg caacttcgta cacactacgt gacatcatcg aagcgttgcc gaaggacagt
      841 aatgcggcat ggaaactcac cagtaatgcc atgtcgatga tggcagatat gcaggcgacg
      901 cgcagacgct gggaagagct gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc
      961 ctgaaaaaat catcgccgtt ttcgtccggt ttttcccgta ttcccctgat gcaatgggcg
     1021 gcaccggaat accgtagcga acagggaaaa atccagtcgc tgagccagca aatggaaaaa
     1081 ttgcgacagc attaccggaa ggtggagaaa caggacattc ccgcgagtca gacggcattt
     1141 gaaaaacagt ggcagcagtg gggggcatcc ggccttgcag ctctcagagt ccagttgctt
     1201 aaacgtgaag aggaattgcg ccggaaggag caggaggata cagaacgtag tcgcgcggag
     1261 cgacgagcgg aacagctcag gaaaaatgac aatgccgtta tggattcagg ggtactgagc
     1321 gcagtcgtta cggggtatgg cgaggccccc atgcctggta acggggaaat gacctgttac
     1381 cttctgcttc acaaccggaa cggagaatac acgctctggg ggaacgagct ggagaaatac
     1441 cgcaccagaa tctttgagcg tgttgattta atgcgtgaca ggagtggtta tatctgtgac
     1501 cgaagcgaaa tcggtcaaca tccaccactg caacgtgttt attcctctgc gacctttgag
     1561 caacttctga cgcaggtttg ccagacatgg ccgcaatata cccgcaatct ccgccagccg
     1621 aagacatggc cggagtcttt ctgtcttgga gaagaccgcc agccagccat gccatcactg
     1681 gcagcaagga aggtggattt tacgcaggga cgtctgctac cgacgctgat gccggtaatg
     1741 agttctgtcg accgggaaac ccgccagctt cagcttttgc tggtcatggg cgtggatgac
     1801 tctctgggcg gtgttgtccg gcttaatggt acgctgtatc ccgcgtttgc cgttccctca
     1861 gccgataaca gccagctggt catcagtgca ctgaccgata aggggttacg gtatgcaggg
     1921 tatggtgttg ccgtaaatca cgatgcagac agccacatat cacctgcgcc ggaacttatg
     1981 gagtttcatc tgaaaacccg tgaggcaccg ctttttgctg ccgtcaacac accagaaaaa
     2041 cagccggatc atctgttccg ctcgctgggt tttaatcgta cctgggacga atggcggcgc
     2101 gaggaagatg cacgaacaca cgccaccgag aggagacatg atcgcggttg gtcacagtga
     2161 atgtgaacaa tattttgttt tgtttaacat cgtgttatgt tcgtcgatgt tcttcgatgt
     2221 tcatcgacag ggatgaatgc aagaagcaca cacataatgc aagcatagtt ttgtttttcc
     2281 gcccccaaaa aatgggggtt cttttttcgc tgcattagag tgcttttgac gcaatttttc
     2341 acttcaatag taccttcgca ctactaacac aaaaaaaacg cagcagggag cgttttaggt
     2401 gccttaaaat cgattttaga tcgtcatctt gatttgtttg tcttccttga agcgaaaatg
     2461 cagggctaca accgtgcgcc cctttttgat cgtctcaacc gttacagcca ggtcgctttt
     2521 tttgttcagt tcgtcaatgc agggtcttaa taattgctgg ttaagtgatt tatatgtttg
     2581 atatttatgc tctatatcaa gcattgagcg aaaatcatcg agagcgataa tacgttcgcc
     2641 agtactgcgg aactgctgga ggagttcgta tatccttata ctgtaacttc ttgataatct
     2701 agatatattc ttgattacaa ctctagtaaa ttgcccttgt agttgtgtca aataaggcat
     2761 tactgcgtca gaaaatgtta tttgtgctct ggcctcgcct ttagcgtact gtgcccgata
     2821 ctggatccag cggaactcct cacgtttctc gtcatccttg agaatgattg acctgtccca
     2881 caaacgatca attgcttcct gtaactgaac ctcggcattt ttccgcccca tgtcagggaa
     2941 tgaatcgaaa tattccgcag ctgtaatggt cattgtcttt tgcaattttg gagattctgc
     3001 atcagcccca gacttcaatc gaccgatgca aagtaacaaa aatcgctgtt cctgtagggt
     3061 caatttataa ctggcttcaa ccagcgcatt ggatttatag gcaattagat ccgacataga
     3121 gcatccctcc ccaaaaggta atgaataaaa gataaacata ccctcaacga aaggcaatgc
     3181 ctttttattt taatatccct ttttctgtga atattacctt aaatcatatt gcctttacct
     3241 taaattcccc cacctaaaca ccttaaatca tattaccttt accttaaatc acattaccta
     3301 tagcattgta acattttgtt ttctataagg aattttggcg ctaaaacaag tattaaaaac
     3361 aagatcttaa aaacaagatc taaaaactta acgacgagcc tgtggataac ttggagtgct
     3421 gaaaacaaaa aaccaataaa aacaatgcgt tgcaaaaaag cagaattgca agttccgaat
     3481 acaccccctc caaccctgtg gaaatccagt attcatgcgg gttcagcgca tatcacctcc
     3541 cgaaaaaccg tgcgtatgtg ctcaatcagg cgacggattc acttccgctt cctcgcccac
     3601 tcgctcgcta acgctcggtc gctgggactg cggcgagcgg acgatacaat cactgtcctg
     3661 gcagcgcttc cagctcgtta atcagcggca gtatgcgcgt cacatcagcc tgtcgtgtca
     3721 cctctgcctt caggaactgg agtaaccagg cacgcaactg cacctcctcc ggctttctga
     3781 cctttgccag caatgctgca cccacaatga ccttctgcgc tgtgtccaga ctcctgctct
     3841 tcgccttcag gcgctgtaat ctggcctcag catcagcaat cttctgttcg agtgttctgc
     3901 tcatgatgta actccgtgcg cgatgaaaaa tcgcatttta gcgcgtcact ggtagtttaa
     3961 aaactgagct ggcataatgc acggaaaatc acgaagtgcg cacttataca atctccactc
     4021 cgtttcgatt gtgtgcgccc tgcggggctg aaagaaaacc acaaaaaggc attacggcag
     4081 aa
//
