LOCUS       NZ_OZ038592             6647 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Escherichia coli isolate 30345_1#279 plasmid 3, complete sequence.
ACCESSION   NZ_OZ038592
VERSION     NZ_OZ038592.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA9068090
            Assembly: GCF_964036945.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Arredondo Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2024) UNIVERSITY OF OSLO, Postboks 1122 Blindern,
            0317 Oslo, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_964036945.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 12:18:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,944
            CDSs (total)                      :: 4,831
            Genes (coding)                    :: 4,651
            CDSs (with protein)               :: 4,651
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 77 of 180
            Pseudo Genes (incomplete)         :: 118 of 180
            Pseudo Genes (internal stop)      :: 46 of 180
            Pseudo Genes (multiple problems)  :: 51 of 180
            Pseudo Genes (short protein)      :: 1 of 180
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OZ038592.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="30345_1#279"
                     /db_xref="taxon:562"
                     /plasmid="3"
     gene            1..192
                     /locus_tag="ABGU87_RS24650"
     CDS             1..192
                     /locus_tag="ABGU87_RS24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            862..1020
                     /locus_tag="ABGU87_RS24655"
     CDS             862..1020
                     /locus_tag="ABGU87_RS24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            1518..1745
                     /locus_tag="ABGU87_RS24660"
     CDS             1518..1745
                     /locus_tag="ABGU87_RS24660"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(1742..3310)
                     /gene="cea"
                     /locus_tag="ABGU87_RS24665"
     CDS             complement(1742..3310)
                     /gene="cea"
                     /locus_tag="ABGU87_RS24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            3570..4007
                     /locus_tag="ABGU87_RS24670"
     CDS             3570..4007
                     /locus_tag="ABGU87_RS24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021532742.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSKGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            4088..4507
                     /locus_tag="ABGU87_RS24675"
     CDS             4088..4507
                     /locus_tag="ABGU87_RS24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4825..5058)
                     /locus_tag="ABGU87_RS24680"
     CDS             complement(4825..5058)
                     /locus_tag="ABGU87_RS24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5065..5583)
                     /locus_tag="ABGU87_RS24685"
     CDS             complement(5065..5583)
                     /locus_tag="ABGU87_RS24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5621..6319)
                     /locus_tag="ABGU87_RS24690"
                     /pseudo=""
     CDS             complement(<5621..6319)
                     /locus_tag="ABGU87_RS24690"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6261..6584)
                     /locus_tag="ABGU87_RS24695"
     CDS             complement(6261..6584)
                     /locus_tag="ABGU87_RS24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(278..365)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          376..934
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4722..6271)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgactaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta
       61 acgctgctgg agaagctcaa cgaactggac gcagatgaac aggccgatat ttgtgaatcg
      121 cttcacgacc acgccgatga gctttaccgc agctgcctcg cacgtttcgg ggatgacggt
      181 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgtg agcggatgcc
      241 gggagctgac aagcccgtca gggcgcgtca gcaggtttta gcgggtgtcg gggcgcagcc
      301 ctgacccagt cacgtagcga tagcggagtg tatactggct taaccatgcg gcatcagtgc
      361 ggattgtatg aaaagtacgc catgccgggt gtgaaatgcc gcacagatgc gtaaggagaa
      421 aatgcacgtc caggcgcttt tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
      481 gactgcggcg agcggtactg actcacacaa aaacggtaac acagttatcc acagaatcag
      541 gggataaggc cggaaagaac atgtgagcaa aagaccagga acaggaagaa ggccacgtag
      601 caggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatag acgctcaagt
      661 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      721 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      781 tcgggaagcg tggcgctttc tcatagctca cgctgttggt atctcagttc ggtgtaggtc
      841 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      901 tccggtaact atcgtcttga gtccaacccg gtaaggcacg ccttaacgcc actggcagca
      961 gccactggta accggattag cagagcgatg atggcacaaa cggtgctaca gagttcttga
     1021 agtagtggcc cgactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     1081 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgttg
     1141 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1201 aagatccttt gatcttttct actgaaccgc gatccccgtc agtttagaag aggaggatgg
     1261 tgcgatggtc cctccctgaa catcacgtat atagttagcc tgacatccaa caaggaggtt
     1321 tatcgcgaat attcccacaa aaaatctttt cctcataact cgatccttat aaaatgaaaa
     1381 gaatatatgg cgaggtttaa tttatgagct taagatacta cataaaaaat attttatttg
     1441 gcctgtactg cacacttata tatatatacc ttataacaaa aaacagcgaa gggtattatt
     1501 tccttgtgtc agataagatg ctatatgcaa tagtgataag cactattcta tgtccatatt
     1561 caaaatatgc tattgaatac atagctttta acttcataaa gaaagatttt ttcgaaagaa
     1621 gaaaaaacct aaataacgcc cccgtagcaa aattaaacct atttatgcta tataatctac
     1681 tttgtttggt cctagcaatc ccatttggat tgctaggact ttttatatca ataaagaata
     1741 attaaatccc taacacctca tttatagtat taagtttatt cttatcaata taggagcata
     1801 gaattcctgt aacaatagca ataccccaaa tacctaatgt agttccagca agcaagctaa
     1861 aaagtaaagc aacaacataa ctcacccctg catctgcagc tttcttctct aatgtaagaa
     1921 atagtggctt ccagtcacct gtatccttaa tttttaggat atcagatacc acatcatatc
     1981 caaaagaaac atgccccgta atcttcaagt acttggcaaa ctgatctaaa tgtttagccc
     2041 agtcatcata cttcaccgat gccaacgcat taaaaatagc atcacgatcg gctttgctga
     2101 atttcttatt taaaacatcc ttgtattttt caaaagcagc gagagcttca ttcacattgc
     2161 cgattttctt acctttagac ttatcagcaa gttcctgtgc cattttcgaa tatttttcac
     2221 catatttttc agtcagcgtt tgataaaagc taactgttgc atcaacagca tccttaatct
     2281 gtgaattaag gagattattc tgtgcttttt tcaaattttc ttcagcttca tgaacacgag
     2341 cgataccggc attacgatta ttactgacct gagaaatagc cttctggatc tgagttatat
     2401 cagcatttat ccggttaata cgtgtttctg atgctgttac ctgtttttgt ttttcttctc
     2461 taatcttacc ggccccaacc cgtcgtctgg ttgcttcaaa aaaaggacgg ttctgaagcg
     2521 gatcattggc tcttggtgat agttttttga ccagctcatc cagttcttta tatttagcgg
     2581 atgcctgagc cagttcattt cgttttccag cgagcgtttt catttctgca tcacgggcat
     2641 ggatactgga gcttaaacga gaattgagag tcttaatctc tccatccatt ttcaccactt
     2701 cagattgtgc agcagaaagt tttttttggg cgatctcaac agctttagct tcttcactca
     2761 atgcagccag tcgtttctct tcagcttcag ccagtttcaa ctggcgttct gtttcagcct
     2821 tctcccgttc aatctcttta cgtcgttgtt ctgcttcctg aaaagccttt tctgctgctt
     2881 ccgcttcttt acgggctttt tcttctgctt tcgcaaggcg caaacgctct gcttccgcct
     2941 gcatagctgc attattagca tgagcaagct ctgttgctga aggcgtacgt gaggcattgt
     3001 gacgaagagc ctcattcacg atatccttca ggcgctgagt cagcgcatcc ctgtttgcct
     3061 ttgctttcgc ctgtgcttcc gctgcagctt ttgcccgggc agcctgctct gcctgtgttt
     3121 tctttaattg agcagtagac catttagcag ttgcatgaat agctgcagaa ctttcacttt
     3181 tactgcctcc ttttccacct ccgccgccag agccactccc gtcaggagta ccattcaaaa
     3241 gagtaataat tacctgtccc ttatcatcat aaggaacacc atctttatag tacgctaccg
     3301 cggtttccat tataaaatcc tctttgactt ttaaaacaat aagttaaaaa taaatactgt
     3361 acatataacc actggtttta tatacagcat aaaagctacg ccgctgcatt ttccctgtca
     3421 agactgtgga cttccatttt tgtgaaaacg atcaaaaaaa cagtctttca caccacgcgc
     3481 tattctcgcc cgatgccaca aaaaccagca caaacattac cgttctcaga cctcattatg
     3541 ttttactgaa actatgagat gagacatcta tgggacactg tcactttatg gcatggcaca
     3601 cactccggga cgcactaaaa atgacaggca gatcgcgttc acagttttac cgtgatatgc
     3661 gcgcaggcct tgtcagttac cgtaccggca gggacggacg acgggagttt gaaaccagtg
     3721 aactgatccg ggcatacggc gaattaaagc agaatgagac accagaaagg cacagtaagg
     3781 gacatgcaga aaatccacat gatcagcaga cagaacgcat tctccgggag ctgaatgagc
     3841 tgaaacaatg cctgacgctg atgcttgagg ataaacaggc acaggatatg gatcgcagac
     3901 gccaggaagc agaacgggaa cagctacaaa atgagatagc ccagctcagg caggcactgg
     3961 aactggaaaa gaaacgggga ttctggtcca ggttgttcgg tcgctgaacg ctgtcagaga
     4021 ctgatgataa aatagtcttc ggataataac tcaccgagaa taaatacttt aaggtaggga
     4081 gacactcatg agacgtaccg gaaacaaact ttgtcttatc gccatgataa cagcaacagt
     4141 agctctcaca gcctgtaccc caaagggcag cgtggaacaa catacccggc attacgtata
     4201 tgcttctgat gacggttttg atcccaactt ttccacccaa aaagccgaca caacacgaat
     4261 gatggtgcct ttttttcggc agttctggga tatgggagct aaagataaag cgacaggaaa
     4321 atcacggagt gatgtgcaac aacgcattca gcagtttcac agccaggaat ttttaaactc
     4381 actccggggc acaactcaat ttgcgggtac tgattaccgc agcaaagacc ttaccccgaa
     4441 aaaatccagg ctgctggctg acacgatttc tgcggtttat ctcgatggct acgagggcag
     4501 acagtaagtg gatttaccat aatcccttaa ttgtacgcac cgctaaaacg cgttcagcgc
     4561 gatcacggca gcagacaggt aaaaatggca acaaaccacc cgaaaaactg ccgcgatcgc
     4621 gcctgataaa ttttaaccgt atgaatacct atgcaaccag agggtacagg ccacattacc
     4681 cccacttaat ccactgaagc tgccattttt catggtttca ccatcccagc gaagggccat
     4741 ccagcgtgcg ttcctgtatt tccggctgac gctcccgttc cagggataac acatgttcgc
     4801 gctcctgtat cagccgttcc tctcttatct ccagttctcg ctgtataact ggctcaagcg
     4861 ttctgtctgc tcgctcaagt gctgcacctg ctgactcaac tgcatgaccc gctcgttcag
     4921 catcgcgttg tcccgttgcg taagcgaaaa catcttctgc aattccacga aggcgctctc
     4981 ccattcgctc agccgctgca tatagtcctg ttgcagctgc tctaaggcgt tcagcaaatg
     5041 tgtttccagc tctgtcactc tgtgtcactc cttcagatgt acccactctt tcccctgaaa
     5101 gggaatcacc tccgctgatt tcccgtacgg aaggacaagg aatttcctgt tcccgtcctg
     5161 cacaaactcc acgccccatg tcttcgcgtt cagtttctgc aatgtctctt cctgcttcct
     5221 gatttcttcc aggttcgcct gtatcctccc tccaagatac cagagcgtcc cgccactcgc
     5281 ggtaaacagg agaaagacta tccccagtaa catcatgccc gtattccctg ccagctttaa
     5341 cacgtccctc ctgtgctgca tcatcgcctc tttcacccct tcccggtgtt tttccagcga
     5401 ttcctctgtc gaggctgtga acagggctat agcgtctctg attttcgtct cgtttgatgt
     5461 cacagcctcg cttacagatt cgccgagcct cctgaactcg ttgttcagca ttttctctgt
     5521 agattcggct ctctctttca gctttttctc gaactccgcg cccgtctgca aaagattgct
     5581 cataaaatgc tcctttcagc ctgatattct tcccgccgtt cggatctgca atgctgatac
     5641 tgcttcgcgt caccctgacc acttccagcc ccgcctcagt gagcgcctga atcacatcct
     5701 gacggccttt tatctctccg gcatggtaaa gtgcatctat acctcgcgtg acgccctcag
     5761 caagcgcctg tttcgtttca ggcaggttat cagggagtgt cagcgtcctg cggttctccg
     5821 gggcgttcgg gtcatgcagc ccgtaatggt gattcaccag cgtctgccag gcatcaattc
     5881 taggcctgtc tgcgcggtcg tagtacggct ggaggcgttt tccggtctgt agctccatgt
     5941 tcggaatgac aaaattcagc tcaagccgtc ccttgtcctg gtgctccacc cacaggatgc
     6001 tgtactgatt tttttcgaga ccgggcatca gtacacgctc aaagctcgcc atcacttttt
     6061 cacgtcctcc cggcggcagc tccttctccg cgaacgacag aacaccggac gtgtatttct
     6121 tcgcaaatgg cgtggcatcg atgagttccc ggacttcttc cggattaccc tgaagcaccg
     6181 ttgcgccttc gcggttacgc tccctcccca gcaggtaatc aaccggacca ctgccaccac
     6241 cttttcccct ggcatgaaat ttaactatca tcccgcgccc cctgttccct gacagccaga
     6301 cgcagccggc gcagctcatc cccgatggcc atcagtgcgg ccaccacctg aacccggtca
     6361 ccggaagacc actgcccgct gttcacctta cgggctgtct gattcaggtt atttccgatg
     6421 gcggccagct gacgcagtaa cggcggtgcc agtgtcggca gttttccgga acgggcaacc
     6481 ggctccccca ggcagacccg ccgcatccat accgccagtt gtttaccctc acagcgttca
     6541 agtaaccggg catgttcatc atcagtaacc cgtattgtga gcatcctctc gcgtttcatc
     6601 ggtatcatta ccccatgaac agaaatcccc cttacacgga ggcatca
//
