LOCUS       NZ_OY978238             6141 bp    DNA     circular CON 21-DEC-2023
DEFINITION  Klebsiella pneumoniae strain KP4482023BGR isolate KP4482023 plasmid
            P_KP4482023_4, complete sequence.
ACCESSION   NZ_OY978238
VERSION     NZ_OY978238.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA114785895
            Assembly: GCF_963854765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Ivanov,I., Teneva,D. and Stoikov,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2023) NCIPD, CMAR, Yanko Sakazov 26 Sofia,
            Bulgaria
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/21/2023 00:14:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,719
            Genes (coding)                    :: 5,494
            CDSs (with protein)               :: 5,494
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 128 of 225
            Pseudo Genes (incomplete)         :: 103 of 225
            Pseudo Genes (internal stop)      :: 28 of 225
            Pseudo Genes (multiple problems)  :: 28 of 225
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY978238.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP4482023BGR"
                     /isolate="KP4482023"
                     /isolation_source="human blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="P_KP4482023_4"
                     /geo_loc_name="Bulgaria"
                     /lat_lon="43.225887 N 27.936721 E"
                     /collection_date="2023-03-16"
     gene            join(6040..6141,1..291)
                     /locus_tag="U9J22_RS29085"
                     /old_locus_tag="KP4482023_28860"
     CDS             join(6040..6141,1..291)
                     /locus_tag="U9J22_RS29085"
                     /old_locus_tag="KP4482023_28860"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            273..611
                     /locus_tag="U9J22_RS29090"
                     /old_locus_tag="KP4482023_28865"
     CDS             273..611
                     /locus_tag="U9J22_RS29090"
                     /old_locus_tag="KP4482023_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            592..2046
                     /locus_tag="U9J22_RS29095"
     CDS             592..2046
                     /locus_tag="U9J22_RS29095"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2328..2609)
                     /locus_tag="U9J22_RS29100"
                     /old_locus_tag="KP4482023_28880"
     CDS             complement(2328..2609)
                     /locus_tag="U9J22_RS29100"
                     /old_locus_tag="KP4482023_28880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            2953..3750
                     /locus_tag="U9J22_RS29105"
                     /old_locus_tag="KP4482023_28885"
     CDS             2953..3750
                     /locus_tag="U9J22_RS29105"
                     /old_locus_tag="KP4482023_28885"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(4157..>4309)
                     /locus_tag="U9J22_RS29110"
                     /pseudo=""
     CDS             complement(4157..>4309)
                     /locus_tag="U9J22_RS29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            4685..5632
                     /locus_tag="U9J22_RS29115"
                     /old_locus_tag="KP4482023_28890"
     CDS             4685..5632
                     /locus_tag="U9J22_RS29115"
                     /old_locus_tag="KP4482023_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     ARG             2954..3747
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          4698..5619
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          593..2043
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attctacttg aagattatat caatggaaaa atttccaccg atgaggtgtt agctatggta
       61 agtgacgaac ccacaacaac ggaatacgac tacgaaatca ccccccgcac tctcggtctt
      121 ggcgggggct ggaatctgcg tttgctggag aacggcgaag aggtcggcgg gggtgttttc
      181 cccttgccgg agcactgcga ttttcgggat gaaaaggcgc tccagacgct actcgatagc
      241 ctctatgagg acgccttagc ggaggcctct gcatggctgg ccagccgtta aagcgcctcc
      301 gctctatcaa gatccgggtc tcagatgcgg agcttgagcg cctgcgggag ctctgcccca
      361 aggcccaact ggcggagtgg atgcgggagc aatgtctcgg tgttgcgcaa ccccagcgcc
      421 gaacgccggc gcctaccgta gatccggcgc tcttgcggca gttggccgga atgggcaaca
      481 acctgaacca gatcgcccgg cgcgttaatt ccggggagtg ggggccgttg gatcggctgc
      541 gcatcatagc ggagctgtcc gctataggcc gcgagctgga ggaactgcac catgatcatc
      601 aaattccata accgtggggt aggccggggc tccgggcctg ttggctactt gttgggccgt
      661 gacgggaacc gggaaggcgc aacgctcgac cggggcaacc ctgcggtgat tgaagccctg
      721 attgattcca gtccctacgc caaaaaatac acctctggcg ttctgtcgtt tgccgaggct
      781 gatttaccca gggcgaccaa ggacaagctg atgagcgact ttgaaaaggc gctcctgccc
      841 ggactggagg gcaaccagta cgctgttttg tgggtagagc accgcgacaa ggggcggctg
      901 gagctgaatt ttgtagtccc gaacatcgaa ttgcagagcg ggaagcggct gcaaccctac
      961 ttccacaagg ccgacaatcc gcgcctcgat gcgtggcgaa ccgtggtaaa cggggagctg
     1021 aaattgcacg acccggacga tccgttaaac cgtcaggccg ttatcacgcc aaaggatttg
     1081 ccgagggggc acaaggaggc cgctcaggcc attacagacg ggcttttacg catggccgga
     1141 gcaggggaga tccagagccg cgatgatgtg cgcaaagcct tgcagggtgc gggtttcagc
     1201 gtggtacgcg aaaccaaaaa cagcctgagc attgccgacc cggacggggg gcggaacatc
     1261 cgattaaagg gggtgctgta tgagcaagat tttagacatg gcgaaggact tcgagcagag
     1321 ctcgaagcag caggccaacg ctatcgagaa gcgtctgacc gaagaattca cgaggcacga
     1381 gaaagctatc tccggggcgt tgaaatcaag cgaggagaaa atgagcgccg ctattgccga
     1441 gcacagccag aaaatgggct ggctgttgct caaaacgtgg atctggatgc tggtcagcat
     1501 cgtcctgttc ttgagcgcaa cgagcgggat cctctggtat cagggggaat tgatagcgga
     1561 gcggcaagcc actctccaag ccttggcaga gaagggcggc aagctaaagc tgacccagtg
     1621 cgggaagaaa ttggagcacc tttgcgtgca gatctccgac cccaatccaa ccgtttgggg
     1681 gaaggatacc tattggatcg tgccgaaggg gtactaaatg acgggattgg agcgggaatt
     1741 gctctgcgcg ttcgaggctt tgcagagcgc atacgagcag cagcacaagg catggcagga
     1801 aagctacgcg aacttggcga gcatgtacga gagcaccaaa caagaggccg agatcagcag
     1861 ggcagcagca gagcgattga cgaggcaggt gaacggctta acgcagcaag tggacggctt
     1921 gagcaagcag ctggagggct tgaccgggct agtcagcaag ttgcaaaaga aagggaacac
     1981 aggctcctga aggcactgga tcgaggctgg gagcgtggag acagatcgcg aggctttgag
     2041 cgttgacgga acaaaacggc cgtcagttat aaaaaaagga aagtgctatc actttccttt
     2101 tatcggcggg aattatcgtt gagttcttct atgccatttt tcatagtagt aactgtaatc
     2161 ttcatctcta ccaccagatt caatccagtc atcatactca gggctatttt tgaaatctga
     2221 atcagacatg ctaactcctt gttattagtt ggcttctatg gaagctagcg caccgtgaag
     2281 ttatgatcaa cgatgtagtc cacatcattg tcgaaatgtg cgttcagcta gtcttcggtg
     2341 ttttctgctt tatttctagc ggcgattctc gcgtctgccc atgcggcaga ctttttagcc
     2401 tgttctcgtt gttcttcggt tgaagactcg ctgttgagaa ctttgttgct cgtttgtttg
     2461 accaccgaat ccaaacctgt tgttgctgca aaatgagcta ggttaacaac tcccttacct
     2521 aacaacttgg cactagcgat tgcggtttct ttagccccaa aagcatcaga cacctcctca
     2581 agcgctttag ataggttaaa aaatcccata ctccccctaa gaatatcatc aataaaattg
     2641 agtgttgctc tgtggataac ttgcagagtt tattaagtat cattgcagca aagatgaaat
     2701 caatgattta tcaaaaatga ttgaaaggtg gttgtaaata atgttacaat gtgtgagaag
     2761 cagtctaaat tcttcgtgaa atagtgattt ttgaagctaa taaaaaacac acgtggaatt
     2821 taggtaatag ttaaagcttg tgcctgattg ttgccacaat tggtcagcag tttgctaggg
     2881 aatgagaggg cagagaatga taagcttagc acttaatttg tatggatata ttgcattaag
     2941 caaggggacg ttatgcgtgt attagcctta tcggctgtgt ttttggtggc atcgattatc
     3001 ggaatgccag cggtagcaaa ggaatggcaa gaaaacaaaa gttggaatgc tcactttact
     3061 gaacataaat cacagggcgt agttgtgctc tggaatgaga ataagcagca aggatttacc
     3121 aataatctta aacgggcgaa ccaagcattt ttacccgcat ctacctttaa aattcccaat
     3181 agcttgatcg ccctcgattt gggcgtggtt aaggatgaac accaagtctt taagtgggat
     3241 ggacagacgc gtgatatcgc cgcttggaat cgtgaccatg acttaattac cgcgatgaag
     3301 tactcagttg tgcctgttta tcaagaattt gcccgccaaa ttggtgaggc acgtatgagt
     3361 aaaatgctgc acgccttcga ttatggcaat gaggatatct cgggcaatgt agacagtttt
     3421 tggctcgatg gtggtattcg catttcggct acccagcaaa tcgctttttt acgcaagctg
     3481 tatcacaaca agctgcacgt ttctgagcgt agtcagcgca tcgtgaaaca agccatgctg
     3541 accgaagcca atggcgacta tattattcgg gctaaaacgg gatactcgac tagtatcgaa
     3601 cctaagattg gctggtgggt tggttgggtt gaacttgatg ataatgtgtg gttttttgcg
     3661 atgaatatgg atatgcccac atcggatggt ttagggctgc gccaagccat cacaaaagaa
     3721 gtgctcaaac aggagaaaat tattccctag aagtggttag cgcgtatttg tgtgaaatag
     3781 ccgtcatata agctgtaaag atatatggac aaaatgctga tgctcgatgc gcttgtagtt
     3841 aatggctcat acttaatcgg tcaggcctaa tcgacttaag ctccgcgcgt taactttcag
     3901 cctgagcatt agcatcacat tcctaaacgt aaaaagccta gccaatttgc taggcttttt
     3961 tgttatctca atggccatgg tcactcaaca tgggggtgtg aataaaggag tggcgatgtc
     4021 agtaaaggtg aatgccgctg cgataaagtg cctagcccat caaggattgt aggctttctt
     4081 ggctgctaaa gcgtttgacg aaaaagtcca agaaggcgct gatattggtt ctccatcatt
     4141 ttcatctacc ggtttactat aggtcaacca ggccgtcctt gccggcgctt gggacgcaga
     4201 cgatggcatc aaatgcctcg ctcaaattcg tttctacaga ggcgctttgg gaccgcattc
     4261 gctttgcttt catgggggca tctgtcaatg tcagacatga atcctccgat ataggttgat
     4321 ttttgatctc ttcaacactt ggtgttgtcc gactagagag acttatatgg atcagtattc
     4381 ttgcactgac aatggtgcca ctcaatccca aggatcgacc tagttagagg cgtagatcaa
     4441 gcaattcaaa acagcgcaga agcagaagga ggtggaaata tgttccaggg tgaaaggtta
     4501 catgcaacta acgttgctct ctaggatgtg cctgctgtaa cgtattgtcc agaaaagaca
     4561 aacccccgtt atcgcttgca tcttggcgga gctgcgaaaa acgggggccg tgacgaatca
     4621 caagagaagt ctatcacatg gatcatgcca aacaacccca cgccgccaat aagcgcggtg
     4681 gtgcttgaga aagtgcgcac agcttgagct gtttcgggag cgcctgcccc ggaaacccta
     4741 ctattccgac gagcttacaa cgggcctacg gattgcggat gttgcccgcg cccttggtgc
     4801 gcgttacatc cagccgaacg ggccaacgca ccgccactgg atcgtttttg acgtagacca
     4861 tgcagccgct acgttatctt gggatgacgt aggcgctcca gcccctaaca ttaccgtcac
     4921 gaacaaggcc aacggccacg cgcatctgat ctacggactg gatacgccga tccgcactgc
     4981 cccggatgga aatgcagccc ctctgcggta tgccgccgcc atcgaggccg cgctccggga
     5041 gaaattaggg gcggatatgg gctactccgg gctgatctgc aagaaccccc tgcatgagca
     5101 ctggctggtg caggtatggg aaccccggct ctacgattta gcctggctat ccgattacct
     5161 cgatctgagc tcctacaatg gccgcaaatc gcttcctgag tacgggcttg ggcgaaactg
     5221 taccctgttc gagaaaacgc gcctgtgggc ctataaagcg atccggcagg gctggccgga
     5281 ttaccccgaa tggttggccg cttgtgttga ccgggcatca ggatataacg cccagttcga
     5341 gcagccgctc cccgcaaacg aagtccggca cacggccaag tccattgcca aatggacgca
     5401 ccagcaccta agccccgcag ggttcagaga ggagcaggcc cggagaggtg cgaagggcgg
     5461 caaggtatcc aagggagggg gaaggccgag caatagcggt aaggacaagt ccgatctatt
     5521 gccggaggtt ctgcggctta aggctcaggg gtacaccaac cgcgatatag cggacgatct
     5581 tcaaatctcc cccagtacgg tgagcgttta cctaaagcgt gatcgtccgt gatcgaaaca
     5641 agccttatca gataatagcc ccctttaggg gggttcctgc gctcttgtct tttagcctta
     5701 aaaaatcctt ttagcgcgga tctttggcga ggtggacaac tgcgcctgtc cgtccatcgc
     5761 ccccctgttt tttcgggggg tgtgggcggc agcagttatc cacggagccg ctctttccct
     5821 tgatcttgct ctagggcttg cagtatcccg cccatggttg aataatgtac taagcaaggc
     5881 tacacagagg cccctctacc tgcttcgcgg tctcgggcct ctgtggcctt gttcaggggt
     5941 gctacgcccc cccgaaaccc cctgtggccc ccgctcgatc cgccccttcg ggacggtctg
     6001 cgcggtggct tcttggctct ggagaatgga cgaggcccta tgtcaacaac acagatagag
     6061 aaagattcga gaaggaaggc agtcaatggc gttatttcta gtttccaact agagggggag
     6121 gccataaccg atgaattcaa a
//
