LOCUS       NZ_OY839923             5872 bp    DNA     circular CON 08-DEC-2023
DEFINITION  Escherichia coli isolate F1059 plasmid pF1059-2, complete sequence.
ACCESSION   NZ_OY839923
VERSION     NZ_OY839923.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113543013
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/08/2023 12:30:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 180
            CDSs (total)                      :: 180
            Genes (coding)                    :: 146
            CDSs (with protein)               :: 146
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 11 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 9 of 34
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY839923.1.
            Complete plasmid sequences of E. coli strain F1059.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F1059"
                     /db_xref="taxon:562"
                     /plasmid="pF1059-2"
     gene            complement(join(5868..5872,1..1048))
                     /locus_tag="U0072_RS00845"
                     /old_locus_tag="FGAF1059_46990"
     CDS             complement(join(5868..5872,1..1048))
                     /locus_tag="U0072_RS00845"
                     /old_locus_tag="FGAF1059_46990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021517772.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGNGGYSHPEGISTTWHSESKLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIDNSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGVPVDDASMFFNSFQPRKFE
                     NNPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(1768..2001)
                     /locus_tag="U0072_RS00850"
                     /old_locus_tag="FGAF1059_47010"
     CDS             complement(1768..2001)
                     /locus_tag="U0072_RS00850"
                     /old_locus_tag="FGAF1059_47010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021520366.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2008..2538)
                     /locus_tag="U0072_RS00855"
                     /old_locus_tag="FGAF1059_47020"
     CDS             complement(2008..2538)
                     /locus_tag="U0072_RS00855"
                     /old_locus_tag="FGAF1059_47020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2576..3274)
                     /locus_tag="U0072_RS00860"
                     /pseudo=""
     CDS             complement(<2576..3274)
                     /locus_tag="U0072_RS00860"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3216..3539)
                     /locus_tag="U0072_RS00865"
                     /old_locus_tag="FGAF1059_47030"
     CDS             complement(3216..3539)
                     /locus_tag="U0072_RS00865"
                     /old_locus_tag="FGAF1059_47030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024188420.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAAWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALIAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3605..3796
                     /locus_tag="U0072_RS00870"
                     /old_locus_tag="FGAF1059_47040"
     CDS             3605..3796
                     /locus_tag="U0072_RS00870"
                     /old_locus_tag="FGAF1059_47040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021539356.1"
                     /translation="MTKQEKTALNMVRFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5000..5866)
                     /locus_tag="U0072_RS00875"
                     /old_locus_tag="FGAF1059_47050"
     CDS             complement(5000..5866)
                     /locus_tag="U0072_RS00875"
                     /old_locus_tag="FGAF1059_47050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     Typing          complement(3891..3972)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          complement(4429..4616)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(1665..3226)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.232"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catcctcttt ctcataaagg gggttgtttt caaatttcct tggttgaaag gagttaaaaa
       61 acatagatgc atcatctaca ggaacaccaa taaactctcc ataataagga tcatctatag
      121 gaaactcttc taatgaaaca taaaacaata cattccagtt gaagatcatt ggatcactat
      181 tatctatttc atggagcaat ctttcatcct tagttaattc tcctggaatt aatacatcag
      241 tatgataaaa accgctgtaa taagaactat taccggtgtg cactgggcat ccaaaacgat
      301 ccaatttaga ttctgaatgc catgttgtag aaatcccttc cggatgtgaa tacccaccgt
      361 taccaacaga ctgaatagtc atgttacgaa tatccgctaa cgcttgctct tgatctttat
      421 agaaaccttt ttctatcgcc agcctcataa aatgccccgg ggcatttaca gaaataagta
      481 ttccccccgg tattcttcta tcatgatttc ttgttctatg agcagaagag taaaaaacat
      541 taacaccagc cttatatatt tttagcccat ctttatcctg aaatggtacg ctttcagtat
      601 aaataacatc ctcttttatt ggatagcact ctggcatatg cagcgaacaa atatattttt
      661 gtatgtccac taactcttct gatggacgag caaatgcaag ttgtggatga ataaaataaa
      721 tgagatgcgc actgacctct cctcttcttg cgcgttcttt aaaacgaact ctttcattac
      781 gcaaaaaatc aaacaaatga ccatcatcat tatatagttg ggggctattt acaatagata
      841 aatggaaatc aagcccttta attttcttgc ttgccaattt accaaataca cagggctgat
      901 tgcctgacac ccattttctt agtaaatctt caattttatc tttagaagtt tcgttaaaga
      961 taaatgaatg aatattatca atatcttttc caaatgaaaa actggatgat aattgtggta
     1021 gtttatttat acgttgttga agattcatga aagctcccac aagacacctc atatattttt
     1081 tatatactga aactacatta tatttattac cgacgtaaaa cgaaataatt tattatcgtt
     1141 accgatattt taaaaaatca ttatttgcac agtgttcgag ttgttagtat cactccttcc
     1201 tgaaaatccg ctccatttac cataacgtcg cattgtacgc accgccaaaa cgcgttcggt
     1261 gcgatcacgg cagcagacag gtaaaaatgg caacaaacca cccgaaaaac tgccgcgatc
     1321 gcacccgata aattttaacc acatgcatag ctatgcagcc atgtgaatca cgcaggaatt
     1381 gccagccaga gactactgaa acggattttt ttcatacttt cggaaaggca gaagacggaa
     1441 ccggagcgga aaaacagaaa ttcatgggaa tgagcgcgat ctggcaataa cggcaaaaca
     1501 cagcaacaaa gacacaccag aatcgcgccc gcatgcgttt taacgcgttc ctgtgttttc
     1561 agcaagctct gactgaagcg cgttcccggt gcccccgaac caaccattat cccaaaaaat
     1621 gcaccggaaa cgcgctgcca acattttagc ctgtctctgg ttaccatccc agcgaagggc
     1681 catccagtgt ccgttcctga atttccggct ggcgctcccg ctccagggat aacgcatatt
     1741 cgcgctcctg tatcagccgc tcatccctta tctccagttc tcgctgtata actggctcaa
     1801 gcgttctgtc cgctcgctca agtgctgcac ctgctgactc aactgcatga ctcgcccgtt
     1861 cagcatcgcg ttgtcccgtt gcgtaagcga aaacattttc tgcaattcca cgaaggcgtt
     1921 ctcccattcg ttcagccgct gcatatagtc ctgttgcagt tgctctaatg cgttcagcaa
     1981 atgtctttcc agctccgtca tgctctgtca ctcctgcacc agtctcactg cgttcagttt
     2041 gccgttgtcc ttcgtccagt tcgtttcggc tttcatcccc ttcggcaaca ccagaaaacg
     2101 tccgttgctg tcctcgtgat aacgcacgcc ccatgtcttc gcgttcagct tctccagact
     2161 ctctttctgc tcgcggagaa tattcatatt cgcgtttatc ttcatgccct ggtaccacag
     2221 cagactcccg gtcgtggcaa acatcagtac tgagacgata atcagccaca gccagcgttt
     2281 cacgctcatt ttcagtacgg ttttccagtg cgcgtccatc gtatcgacgg tcgttttctg
     2341 atacctctcc agcagttctt tcagggctgt tgtatggtcg ttgatagcgt tcctgattaa
     2401 agattcgttt gaggtcagtt ccctgtttac agattcttcg agcttcctga actcgtcgtt
     2461 cagcattttc tctgtagatt cggctctctc tttcagcttt ttctcgaact ccgcgcccgt
     2521 ctgtaaaaga ttgctcataa aacgctcctt tcagtctgat attcctcccg ccgttcgggt
     2581 ctgcaatgct gatactgctt cgcgtcaccc tgaccacttc aagccccgcc tctgtgagcg
     2641 cctgaatcac atcctgacgg ccttttatct ctccggcatg gtaaagcgcg tctatcccgc
     2701 gtgtgacgcc ctcagcaagc gcctgtttcg tttccggcag gttatcagga agggtcagcg
     2761 tccggcggtt ctccggggcg ttggggtcat gcagaccgta atggtgattc accagcgtct
     2821 gccaggcatc aattctcggc ctgtctgccc ggtcgtagta cggctggagg cgttttccgg
     2881 tctgtagctc catgttcgga atgacaaaat tcagctcaag ccgtcccttg tcctggtgct
     2941 ccacccacag gatgctgtac tgatttttct cgagaccggg catcagtaca cgctcaaagc
     3001 tcgccatcac tttttcacgc cctccaggcg gcagctcctt ctccgcgaac gacagaacac
     3061 ctgacgtgta tttcttcgca aatggcgtgg catcgatgag ttcccggacc tcttccggat
     3121 tcccctgaag caccgttgcc ccttcccggt tacgctccct tcccagcagg taatcaaccg
     3181 gaccactgcc accacctttt cccctggcat gaaacttaac tatcatcccg cgccccctgt
     3241 tccctgaccg ccagacgcag ccggcgcagc tcatccccga tggctatcag tgcggccacc
     3301 acctgaaccc ggtcaccgga agaccactgc ccgctgttca ccttcctcgc tgtctggtta
     3361 aggttattcc cgatagcggc cagctgacgc agtaacggcg gtgccagtgt cggcagcttc
     3421 ccggaacggg caacaggctc acccaggcag acccgccgca tccatgccgc cagttgttta
     3481 ccctcacagc gttccagtaa acgggcatgc tcatcatcag tcacccgtat cgtgagcatc
     3541 ctctcgcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca
     3601 tccagtgact aaacaggaaa aaaccgccct taacatggtc cgctttatca gaagccagac
     3661 attaacgctg ctggagaagc tcaacgaact ggacgcggat gaacaggcag acatctgtga
     3721 atcgcttcac gaccacgccg atgagcttta ccgcagttgc ctcgcacgct tcggggatga
     3781 cggtgaaaac cgctgacaca tgcagctctc ggagacggtc acagcttgtc tgtgagcgga
     3841 tgccgggagc agacaagccc gccagggcgc gtcaggccgt tgacctttgg ggtgtcgggg
     3901 cgaagccctg acccagtcac gtagcgatag cggagtgtat actggcttaa tcattcggca
     3961 tcagtgcgga ttgtatgaaa agtgcgccat gtcgggtgtg aaatgccgca cagatgcgta
     4021 aggcgaaaat gccagttcag gcgctcttcc gcttcctcgc tcactgactc gctacgctcg
     4081 gtcgttcggc tgcggcgagc ggactctcct ttctcaaaaa cgctaaccct gttatccaca
     4141 gaacccgggg ataaaccagg aaagaacatg tgcgcaaaaa acgaaacccc ggaaaaggcc
     4201 gcgccggagg cgctttttcc ataggctccg cccccctgac ggacttcaca aaacagacac
     4261 tttttcggtg catctgtggg agccttgcgg ctcaaccatg aaatccgcca gcggtggcga
     4321 aacccgacgt ggcttaaaga tcatcaccgt ttccggtgaa tcgctccctc ctgcgccccc
     4381 acgttcccgc catatcgatt accggatacc ttttccgcct ttctccctta cgggacacgt
     4441 ggcgctttct catagctcac gtcttctggt atctcggggc gacttaggca gttcgctccg
     4501 ggctgggctg tatgcaggaa ccccccgttc agcccgactg ctgcgccttc tccggtaact
     4561 gttctcttgg gtccaacccg gaaaagacac cacaaagcgc cactggcagc agccattggt
     4621 aaaagtcatg tgcgagatat ttagagagta cttgaagtgg ctgccggagt gcggctacac
     4681 tagaagaaca attttggtga ctcgtctcgc ataagacagt tacctggtta agcagtctgc
     4741 aagctgactt aaccttcgat caaaccgccc tgcaaggcgg ttttttcgtt tacaggcagc
     4801 agattacgcg cagaaaaaaa ggatctcaag aggatccttt gatctttttg ttacaaacgt
     4861 ctctatttga ctcccctgag agcgttttag cgcgcctctg ccccccttgg ttacattccg
     4921 acaccagaaa cgctcccgtt ttttacgtgc atttggcacg gtatcgatgg aggccggggg
     4981 ggcaatcctg aacagaatgt tactcctctg agtattttct gttaagaaaa tatacactta
     5041 gcacagttac aataaatcct accccctgta agaatgtgaa ttgttcatcc aaaaacaacc
     5101 atgacaaaaa taatgcaact agcggaataa agtacaacat tatccctgcc aaatgaactg
     5161 ggagccaacg caaggagttc aagtaaaaac aaaaagctaa aaaatattgt aatattccag
     5221 atgaaaaaac aataatcacg gattccaacg gaagataaat tgaggattta gatataaaaa
     5281 taaagagcga tgacagtata agggagaatg tcaactgtcc gctcaataat gtgataggct
     5341 caaacgattt aataaggcga gacgatataa caacataaaa tgctgccgat aacacaccgg
     5401 caacaacaag acaatatcca acaatattat tcgatccatt actgatgtct ggcaaggcta
     5461 ccaaaatact gccaacaatt gcacctataa ggaacaatat aaaatattgc ggggaattaa
     5521 ttttaactga gcaaataaga acagacaaca ccaatataaa cactgattca agtgaaaaaa
     5581 taattgatgc gcttcctgct gaaataaaat aaagtccgta gagtgtaaaa gtatacgcta
     5641 agaatggttc aaaaacgcct gtcgcataag caagtatcgc ttttgagttt aatgggagtt
     5701 tttttctttt tataaaaaga actatccaag atagtattac tgcactacaa atctgccaga
     5761 aaaacacccc tccggcatct agttcttcaa ctcctttttt gctcattacg atagctgccc
     5821 cccatgagaa cgctgctaaa aaagcaaaaa gcattccttt aatcatatta at
//
