LOCUS       NZ_OY757138             3918 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Escherichia coli isolate F801 plasmid pF801-5, complete sequence.
ACCESSION   NZ_OY757138
VERSION     NZ_OY757138.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542911
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 19:11:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 526
            CDSs (total)                      :: 522
            Genes (coding)                    :: 441
            CDSs (with protein)               :: 441
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 26 of 81
            Pseudo Genes (incomplete)         :: 66 of 81
            Pseudo Genes (internal stop)      :: 9 of 81
            Pseudo Genes (multiple problems)  :: 17 of 81
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY757138.1.
            Complete plasmid sequences of E. coli strain F801.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F801"
                     /db_xref="taxon:562"
                     /plasmid="pF801-5"
     gene            complement(join(3850..3918,1..378))
                     /locus_tag="SBO99_RS02620"
                     /old_locus_tag="FGAF801_52060"
     CDS             complement(join(3850..3918,1..378))
                     /locus_tag="SBO99_RS02620"
                     /old_locus_tag="FGAF801_52060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_021537414.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLAKDSA"
                     /name="conjugal transfer protein TraD"
     gene            550..2100
                     /locus_tag="SBO99_RS02625"
                     /old_locus_tag="FGAF801_52070"
     CDS             550..2100
                     /locus_tag="SBO99_RS02625"
                     /old_locus_tag="FGAF801_52070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001624483.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKASI
                     PQTAGERKAALVALRSRWADVQNEHLARYGHESRVDHRSLKEQGINRTPEVHLGPVQAA
                     SLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPELLLQYR
                     KVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARYLDKLGR
                     NVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHERNEFRNKL
                     QQAESEWDKENAAFKRTEGYKYVGDLDRYREREILAAASLENTRQKVAEEVSVARSQML
                     SLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALRIQRSWAREASRSNERDQDMER"
                     /name="MobA/MobL family protein"
     gene            2557..3588
                     /locus_tag="SBO99_RS02630"
                     /old_locus_tag="FGAF801_52080"
     CDS             2557..3588
                     /locus_tag="SBO99_RS02630"
                     /old_locus_tag="FGAF801_52080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_317721232.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSKNQADKGKVG
                     GKNSGVVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDSSGEWLAKKQI
                     IKY"
                     /name="replication initiation protein"
     Typing          2558..3333
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.654"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          551..2097
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.629"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggtagctct gcgattcctg cgagtgcgcc taccagttcg gcagcactaa actttggttt
       61 tccggttttg ctgtccacca gtcctgccac aatcatgagg cctgcggcct tgtagagttc
      121 atgatcccgc gcctttcgtt ctgcccttgc ggctgccttc cgttctgccg ctatgattgc
      181 cgttaccctg gcttttgctg cggctgcttt ctctgcggtt ttttctgccc gtatcagtgc
      241 ggacaacgtt ttttcatccg tcgttgttcg gttttttttc tctgccagta acacaagtaa
      301 tctctgctgc tctgacggtg atttcagtcc gttgatgtag gcaatttttg cacttaacca
      361 ctcttcacga tttgtcattc ttacctctgc acttttgccg ttaatgtgag ttgttattgt
      421 gcataatatc accttgtctg cctgcgtgtg agaccgtaaa atcatccctg attaattcag
      481 ggatgcgcac ttagtagttt ctctccttcg tcgagaaact gtgcgctcct gcggagggct
      541 aaaaggcaga tggcaagcta ccattgcacc gtcaaagctg gagcaaaagg cagtgcctta
      601 aaacatgctg attacatttc cagatccggc gaatataaga gctacaaatc gcgggaggat
      661 ctggaatttt cgtcatcagg taacatgcca tcatgggcga agaaaaatcc cgcagagcta
      721 tggaaagccg cagatgaatt tgaacgaaaa aacgggactg cataccgtga aattgagatc
      781 gcgttaccgc gtgaactgac acgcgaacaa cggatagaac ttgttgaaga tttcgtgcaa
      841 aaggaacttg gcgatcgcca tgcctaccag tacgcgatcc acaatccgcc aggtgcaatc
      901 gacggaaagg aacagccaca cgcgcatatc atgttttgcg agcggattaa tgatggtatt
      961 gaacgcgatc cgcagcaatt ttttaaacgt gccaacagca aatcaccaga acggggaggc
     1021 gcaaaaaaag cgagtattcc ccagacagcc ggagagcgaa aagccgccct tgttgcgctg
     1081 cgttcccgct gggcagatgt gcagaatgag caccttgcgc gatacggtca cgagtcgcgc
     1141 gtagatcatc gttctctgaa agaacagggt atcaatcgca cgccggaagt tcatcttggc
     1201 cccgttcagg ccgccagtct caatggtgag cagattgttg caatccagga acggaggaat
     1261 gccgagcggg aactcaagac cgccagagat gccgcaaatg ccattcagca ggaacaggag
     1321 caaaagcaaa aaatcaaggc agttgagcct gttcgttcag cccgttcacc ggagttattg
     1381 cttcagtacc gcaaggttat gaaaacggtc attcagggag aagcaaggct tgctcgttta
     1441 ggggatgcca atcccaatgc gctcaaagaa cataagctgc ttcagaatgc gaaagcaaaa
     1501 aaagactctc tttccgaatg gagcaggcga atatatgaag gggcacggta tttagacaaa
     1561 ctggggcgta atgtcgtttc cgcacaaagg gagctgcggg agctacagga acaacgcaat
     1621 gctttgaatg ggattcgtgg cctgtttcgg ggggctgaca aaagggagat tgatgcgcgt
     1681 attctggagc aaaaaagtgt gctggaaacc gctgaacatg aaaggaatga attcaggaac
     1741 aagctacagc aagccgaatc cgagtgggac aaagaaaacg ctgcttttaa gcgtaccgag
     1801 ggatataagt acgttgggga tctggacagg tacagagaac gtgagattct ggccgcggca
     1861 tctttagaga ataccaggca aaaggttgct gaagaagtat ccgttgcccg tagccagatg
     1921 ttatctctgg agcctgaact cagtatatct ggagatgaaa aagctcaaat gcgacatgag
     1981 ttactggcag aaatggcgca ggaacggcag caacaggagg aaaaggcact gcgtatacag
     2041 cgatcctggg ccagggaggc atcacgtagt aacgaacgtg atcaagatat ggagcgttga
     2101 aaatgtagca atatcgggac tgcgccccga accccgccaa cgtttcacgg aacgcgaaaa
     2161 aattccgcgt cccgttcacc gttttctgat gagaaagttt gctgttttga cccgttcgcc
     2221 cttctgaacg ctcgaaacag atcgcgcatc gctttcgtgc atagttatgc aacgctctga
     2281 aaacgattct gacgcgtttt tctggtttgg cctggtgttt tcctgtcttt ttgcgttttt
     2341 tgcgtcagga agcgactgag ggcgttttac ggggtacgta caacgggagt tatggtaaat
     2401 ggatcggttt tgcgggaagg ctcgacaggt tttgtcgttg ggggtagtgt aagcgactga
     2461 aaaacaaacg cccctgaaat tcgtgtctct ccgccaagat tgtcacgaaa attcaagggg
     2521 cgtgtgggtc gtaacacacg gaggaaatcc taacacatga gcgccgcgct tcaatacttc
     2581 gaagaaaatt taccccaccg tccctatcac acggatgatc tcgcttttgg tcttcgcatc
     2641 tccggcaaag ggcgtgcgct tcttgcgcgg tacatccagc agaaccagcc tcatgcgcag
     2701 ttctggctgg tttttgacgt tgaccgtgta ggagctgcga ttgactggag cgaccggaac
     2761 gcccccgcgc cgaacatcac cgtaaaaaat cctgtgaacg gacacgctca cctgctctac
     2821 gcactcaaca tcgccgtgag aaccgcgcct gatgcctcgg ttaaggcgct gaaatatgcc
     2881 gcagcggttg aacgttcgct gtgtgagaaa ctttgtgctg atgtgaacta cagcggcctg
     2941 atttgcaaaa atccgttcca cctggaatgg caggtgatgg agtggcgcga ggacgcctat
     3001 accctcgatg aactggcaga ttatctcgat ttgagcgcct cagagcgtcg tagcatcgat
     3061 aaacattacg ggatggggcg aaactgccac ctgttcgaaa tgacgcgcaa atgggcttac
     3121 agggcgattc gtcagggctg gccagcgttc tcacagtggc ttgaagcggt gatccagcgt
     3181 gtcgaaatgt acaatgcatc gcttcccgtt ccgctttcac tggctgaatg tcgggctatt
     3241 ggcaagagca ttgcgaaata cacgcacagg aacttcacgc cggaaacttt cgcacagtat
     3301 gtggctgata cgcacacgcc agaaattcag gccgcacgag gtcgcaaggg cggtaaggcc
     3361 aattcgtcaa aaaatcaggc ggacaaaggg aaagttggcg ggaaaaacag cggtgttgtc
     3421 cggtggacgg ctaacgatga taaacgtcgt agggcgcttg atatgtacat cttgggagcg
     3481 tcaacagaag atattgcagt ggcggttggt gttagcagtc ggactattcg acgctggatg
     3541 gatagctctg gagaatggct agcaaagaaa caaataatta aatattagtt tacaaaaaaa
     3601 gcaacaaacc ccctggtttt tgttaatggg ttgttaaatt tgcggacacg aagcaagcct
     3661 atatcagata acagcgcctt tcggcgtctt tttgagcagc gggctttccg gcggttttct
     3721 cctttcagcc cagcaatggt gcggcttacc gcatgattga ggtggttgtg ctcgccgcag
     3781 tctcatgacc gagcgtagcg agcgaatgag cgaggaagca ccagggaacc ggaacgtgaa
     3841 cggaggctct tacgcactgt ctttggcgag tagctctttc ccgcgttttt cccattcctg
     3901 ccatttggga tggttgcg
//
