LOCUS       NZ_OY757109             3918 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Escherichia coli isolate S143 plasmid pS143-5, complete sequence.
ACCESSION   NZ_OY757109
VERSION     NZ_OY757109.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542893
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 18:58:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 525
            CDSs (total)                      :: 521
            Genes (coding)                    :: 441
            CDSs (with protein)               :: 441
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 64 of 80
            Pseudo Genes (internal stop)      :: 9 of 80
            Pseudo Genes (multiple problems)  :: 17 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY757109.1.
            Complete plasmid sequences of E. coli strain S143.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="S143"
                     /db_xref="taxon:562"
                     /plasmid="pS143-5"
     gene            complement(join(3468..3918,1..581))
                     /locus_tag="SBO94_RS02595"
     CDS             complement(join(3468..3918,1..581))
                     /locus_tag="SBO94_RS02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_317721232.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSKNQADKGKVG
                     GKNSGVVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDSSGEWLAKKQI
                     IKY"
                     /name="replication initiation protein"
     gene            complement(1038..2588)
                     /locus_tag="SBO94_RS02600"
                     /old_locus_tag="FGAS143_51460"
     CDS             complement(1038..2588)
                     /locus_tag="SBO94_RS02600"
                     /old_locus_tag="FGAS143_51460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001624483.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKASI
                     PQTAGERKAALVALRSRWADVQNEHLARYGHESRVDHRSLKEQGINRTPEVHLGPVQAA
                     SLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPELLLQYR
                     KVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARYLDKLGR
                     NVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHERNEFRNKL
                     QQAESEWDKENAAFKRTEGYKYVGDLDRYREREILAAASLENTRQKVAEEVSVARSQML
                     SLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALRIQRSWAREASRSNERDQDMER"
                     /name="MobA/MobL family protein"
     gene            2760..3206
                     /locus_tag="SBO94_RS02605"
                     /old_locus_tag="FGAS143_51470"
     CDS             2760..3206
                     /locus_tag="SBO94_RS02605"
                     /old_locus_tag="FGAS143_51470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_021537414.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLAKDSA"
                     /name="conjugal transfer protein TraD"
     Typing          complement(2..581)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.246"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(3740..3918)
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="92.0"
                     /evalue="1.56e-88"
     Typing          complement(1042..2588)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.629"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccctgacgaa tcgccctgta agcccatttg cgcgtcattt cgaacaggtg gcagtttcgc
       61 cccatcccgt aatgtttatc gatgctacga cgctctgagg cgctcaaatc gagataatct
      121 gccagttcat cgagggtata ggcgtcctcg cgccactcca tcacctgcca ttccaggtgg
      181 aacggatttt tgcaaatcag gccgctgtag ttcacatcag cacaaagttt ctcacacagc
      241 gaacgttcaa ccgctgcggc atatttcagc gccttaaccg aggcatcagg cgcggttctc
      301 acggcgatgt tgagtgcgta gagcaggtga gcgtgtccgt tcacaggatt ttttacggtg
      361 atgttcggcg cgggggcgtt ccggtcgctc cagtcaatcg cagctcctac acggtcaacg
      421 tcaaaaacca gccagaactg cgcatgaggc tggttctgct ggatgtaccg cgcaagaagc
      481 gcacgccctt tgccggagat gcgaagacca aaagcgagat catccgtgtg atagggacgg
      541 tggggtaaat tttcttcgaa gtattgaagc gcggcgctca tgtgttagga tttcctccgt
      601 gtgttacgac ccacacgccc cttgaatttt cgtgacaatc ttggcggaga gacacgaatt
      661 tcaggggcgt ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagcct
      721 tcccgcaaaa ccgatccatt taccataact cccgttgtac gtaccccgta aaacgccctc
      781 agtcgcttcc tgacgcaaaa aacgcaaaaa gacaggaaaa caccaggcca aaccagaaaa
      841 acgcgtcaga atcgttttca gagcgttgca taactatgca cgaaagcgat gcgcgatctg
      901 tttcgagcgt tcagaagggc gaacgggtca aaacagcaaa ctttctcatc agaaaacggt
      961 gaacgggacg cggaattttt tcgcgttccg tgaaacgttg gcggggttcg gggcgcagtc
     1021 ccgatattgc tacattttca acgctccata tcttgatcac gttcgttact acgtgatgcc
     1081 tccctggccc aggatcgctg tatacgcagt gccttttcct cctgttgctg ccgttcctgc
     1141 gccatttctg ccagtaactc atgtcgcatt tgagcttttt catctccaga tatactgagt
     1201 tcaggctcca gagataacat ctggctacgg gcaacggata cttcttcagc aaccttttgc
     1261 ctggtattct ctaaagatgc cgcggccaga atctcacgtt ctctgtacct gtccagatcc
     1321 ccaacgtact tatatccctc ggtacgctta aaagcagcgt tttctttgtc ccactcggat
     1381 tcggcttgct gtagcttgtt cctgaattca ttcctttcat gttcagcggt ttccagcaca
     1441 cttttttgct ccagaatacg cgcatcaatc tcccttttgt cagccccccg aaacaggcca
     1501 cgaatcccat tcaaagcatt gcgttgttcc tgtagctccc gcagctccct ttgtgcggaa
     1561 acgacattac gccccagttt gtctaaatac cgtgcccctt catatattcg cctgctccat
     1621 tcggaaagag agtctttttt tgctttcgca ttctgaagca gcttatgttc tttgagcgca
     1681 ttgggattgg catcccctaa acgagcaagc cttgcttctc cctgaatgac cgttttcata
     1741 accttgcggt actgaagcaa taactccggt gaacgggctg aacgaacagg ctcaactgcc
     1801 ttgatttttt gcttttgctc ctgttcctgc tgaatggcat ttgcggcatc tctggcggtc
     1861 ttgagttccc gctcggcatt cctccgttcc tggattgcaa caatctgctc accattgaga
     1921 ctggcggcct gaacggggcc aagatgaact tccggcgtgc gattgatacc ctgttctttc
     1981 agagaacgat gatctacgcg cgactcgtga ccgtatcgcg caaggtgctc attctgcaca
     2041 tctgcccagc gggaacgcag cgcaacaagg gcggcttttc gctctccggc tgtctgggga
     2101 atactcgctt tttttgcgcc tccccgttct ggtgatttgc tgttggcacg tttaaaaaat
     2161 tgctgcggat cgcgttcaat accatcatta atccgctcgc aaaacatgat atgcgcgtgt
     2221 ggctgttcct ttccgtcgat tgcacctggc ggattgtgga tcgcgtactg gtaggcatgg
     2281 cgatcgccaa gttccttttg cacgaaatct tcaacaagtt ctatccgttg ttcgcgtgtc
     2341 agttcacgcg gtaacgcgat ctcaatttca cggtatgcag tcccgttttt tcgttcaaat
     2401 tcatctgcgg ctttccatag ctctgcggga tttttcttcg cccatgatgg catgttacct
     2461 gatgacgaaa attccagatc ctcccgcgat ttgtagctct tatattcgcc ggatctggaa
     2521 atgtaatcag catgttttaa ggcactgcct tttgctccag ctttgacggt gcaatggtag
     2581 cttgccatct gccttttagc cctccgcagg agcgcacagt ttctcgacga aggagagaaa
     2641 ctactaagtg cgcatccctg aattaatcag ggatgatttt acggtctcac acgcaggcag
     2701 acaaggtgat attatgcaca ataacaactc acattaacgg caaaagtgca gaggtaagaa
     2761 tgacaaatcg tgaagagtgg ttaagtgcaa aaattgccta catcaacgga ctgaaatcac
     2821 cgtcagagca gcagagatta cttgtgttac tggcagagaa aaaaaaccga acaacgacgg
     2881 atgaaaaaac gttgtccgca ctgatacggg cagaaaaaac cgcagagaaa gcagccgcag
     2941 caaaagccag ggtaacggca atcatagcgg cagaacggaa ggcagccgca agggcagaac
     3001 gaaaggcgcg ggatcatgaa ctctacaagg ccgcaggcct catgattgtg gcaggactgg
     3061 tggacagcaa aaccggaaaa ccaaagttta gtgctgccga actggtaggc gcactcgcag
     3121 gaatcgcaga gctaccgcgc aaccatccca aatggcagga atgggaaaaa cgcgggaaag
     3181 agctactcgc caaagacagt gcgtaagagc ctccgttcac gttccggttc cctggtgctt
     3241 cctcgctcat tcgctcgcta cgctcggtca tgagactgcg gcgagcacaa ccacctcaat
     3301 catgcggtaa gccgcaccat tgctgggctg aaaggagaaa accgccggaa agcccgctgc
     3361 tcaaaaagac gccgaaaggc gctgttatct gatataggct tgcttcgtgt ccgcaaattt
     3421 aacaacccat taacaaaaac cagggggttt gttgcttttt ttgtaaacta atatttaatt
     3481 atttgtttct ttgctagcca ttctccagag ctatccatcc agcgtcgaat agtccgactg
     3541 ctaacaccaa ccgccactgc aatatcttct gttgacgctc ccaagatgta catatcaagc
     3601 gccctacgac gtttatcatc gttagccgtc caccggacaa caccgctgtt tttcccgcca
     3661 actttccctt tgtccgcctg attttttgac gaattggcct taccgccctt gcgacctcgt
     3721 gcggcctgaa tttctggcgt gtgcgtatca gccacatact gtgcgaaagt ttccggcgtg
     3781 aagttcctgt gcgtgtattt cgcaatgctc ttgccaatag cccgacattc agccagtgaa
     3841 agcggaacgg gaagcgatgc attgtacatt tcgacacgct ggatcaccgc ttcaagccac
     3901 tgtgagaacg ctggccag
//
