LOCUS       NZ_OY757104             5873 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Escherichia coli isolate S159 plasmid pS159-4, complete sequence.
ACCESSION   NZ_OY757104
VERSION     NZ_OY757104.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542986
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 18:50:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 361
            CDSs (total)                      :: 358
            Genes (coding)                    :: 323
            CDSs (with protein)               :: 323
            Genes (RNA)                       :: 3
            tRNAs                             :: 3
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 15 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            Pseudo Genes (multiple problems)  :: 12 of 35
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY757104.1.
            Complete plasmid sequences of E. coli strain S159.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="S159"
                     /db_xref="taxon:562"
                     /plasmid="pS159-4"
     gene            join(5793..5873,1..52)
                     /locus_tag="SBO90_RS01760"
                     /pseudo=""
     CDS             join(5793..5873,1..52)
                     /locus_tag="SBO90_RS01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(409..1284)
                     /locus_tag="SBO90_RS01765"
                     /old_locus_tag="FGAS159_48040"
     CDS             complement(409..1284)
                     /locus_tag="SBO90_RS01765"
                     /old_locus_tag="FGAS159_48040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430975.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_000609294.1"
                     /translation="MINMIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSA
                     VILSWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLES
                     VFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLS
                     AAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGIL
                     QYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSV
                     YFLNRKYSEE"
                     /name="DMT family transporter"
     gene            complement(1277..2329)
                     /locus_tag="SBO90_RS01770"
                     /old_locus_tag="FGAS159_48050"
     CDS             complement(1277..2329)
                     /locus_tag="SBO90_RS01770"
                     /old_locus_tag="FGAS159_48050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021517772.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGNGGYSHPEGISTTWHSESKLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIDNSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGVPVDDASMFFNSFQPRKFE
                     NNPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(2821..2964)
                     /locus_tag="SBO90_RS01775"
                     /old_locus_tag="FGAS159_48060"
     CDS             complement(2821..2964)
                     /locus_tag="SBO90_RS01775"
                     /old_locus_tag="FGAS159_48060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_318039577.1"
                     /translation="MALRWDGNQRQAKMLAARFRCIFWDNGWFGGTGNALQSELAENTG
                     TR"
                     /name="hypothetical protein"
     gene            complement(3049..3282)
                     /locus_tag="SBO90_RS01780"
                     /old_locus_tag="FGAS159_48070"
     CDS             complement(3049..3282)
                     /locus_tag="SBO90_RS01780"
                     /old_locus_tag="FGAS159_48070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021520366.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3289..3819)
                     /locus_tag="SBO90_RS01785"
                     /old_locus_tag="FGAS159_48080"
     CDS             complement(3289..3819)
                     /locus_tag="SBO90_RS01785"
                     /old_locus_tag="FGAS159_48080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3857..4555)
                     /locus_tag="SBO90_RS01790"
                     /pseudo=""
     CDS             complement(<3857..4555)
                     /locus_tag="SBO90_RS01790"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4497..4820)
                     /locus_tag="SBO90_RS01795"
                     /old_locus_tag="FGAS159_48090"
     CDS             complement(4497..4820)
                     /locus_tag="SBO90_RS01795"
                     /old_locus_tag="FGAS159_48090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024188420.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAAWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALIAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4886..5077
                     /locus_tag="SBO90_RS01800"
                     /old_locus_tag="FGAS159_48100"
     CDS             4886..5077
                     /locus_tag="SBO90_RS01800"
                     /old_locus_tag="FGAS159_48100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021539356.1"
                     /translation="MTKQEKTALNMVRFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5232..5576)
                     /locus_tag="SBO90_RS01805"
     CDS             complement(5232..5576)
                     /locus_tag="SBO90_RS01805"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_317731062.1"
                     /translation="MSRKAPTDAPKKCLFCEVRQGGGAYGKSASGAAFSGVSFFAHMFF
                     PGLSPGSVDNRVSVFEKGESARRSRTTERSESVSEEAEERLNWHFLLTHLCGISHPTWR
                     TFHTIRTDAE"
                     /name="hypothetical protein"
     Typing          complement(5172..5253)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          complement(5710..5873)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="87.0"
                     /evalue="1.02e-80"
     Typing          complement(2946..4507)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.232"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaagcgcca ctggcagcag ccattggtaa aagtcatgtg cgagatattt agagagtact
       61 tgaagtggct gccggagtgc ggctacacta gaagaacaat tttggtgact cgtctcgcat
      121 aagacagtta cctggttaag cagtctgcaa gctgacttaa ccttcgatca aaccgccctg
      181 caaggcggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagag
      241 gatcctttga tctttttgtt acaaacgtct ctatttgact cccctgagag cgttttagcg
      301 cgcctctgcc ccccttggtt acattccgac accagaaacg ctcccgtttt ttacgtgcat
      361 ttggcacggt atcgatggag gccggggggg gcaatcctga acagaatgtt actcctctga
      421 gtattttctg ttaagaaaat atacacttag cacagttaca ataaatccta ccccctgtaa
      481 gaatgtgaat tgttcatcca aaaacaacca tgacaaaaat aatgcaacta gcggaataaa
      541 gtacaacatt atccctgcca aatgaactgg gagccaacgc aaggagttca agtaaaaaca
      601 aaaagctaaa aaatattgta atattccaga tgaaaaaaca ataatcacgg attccaacgg
      661 aagataaatt gaggatttag atataaaaat aaagagcgat gacagtataa gggagaatgt
      721 caactgtccg ctcaataatg tgataggctc aaacgattta ataaggcgag acgatataac
      781 aacataaaat gctgccgata acacaccggc aacaacaaga caatatccaa caatattatt
      841 cgatccatta ctgatgtctg gcaaggctac caaaatactg ccaacaattg cacctataag
      901 gaacaatata aaatattgcg gggaattaat tttaactgag caaataagaa cagacaacac
      961 caatataaac actgattcaa gtgaaaaaat aattgatgcg cttcctgctg aaataaaata
     1021 aagtccgtag agtgtaaaag tatacgctaa gaatggttca aaaacgcctg tcgcataagc
     1081 aagtatcgct tttgagttta atgggagttt ttttcttttt ataaaaagaa ctatccaaga
     1141 tagtattact gcactacaaa tctgccagaa aaacacccct ccggcatcta gttcttcaac
     1201 tccttttttg ctcattacga tagctgcccc ccatgagaac gctgctaaaa aagcaaaaag
     1261 cattccttta atcatattaa tcatcctctt tctcataaag ggggttgttt tcaaatttcc
     1321 ttggttgaaa ggagttaaaa aacatagatg catcatctac aggaacacca ataaactctc
     1381 cataataagg atcatctata ggaaactctt ctaatgaaac ataaaacaat acattccagt
     1441 tgaagatcat tggatcacta ttatctattt catggagcaa tctttcatcc ttagttaatt
     1501 ctcctggaat taatacatca gtatgataaa aaccgctgta ataagaacta ttaccggtgt
     1561 gcactgggca tccaaaacga tccaatttag attctgaatg ccatgttgta gaaatccctt
     1621 ccggatgtga atacccaccg ttaccaacag actgaatagt catgttacga atatccgcta
     1681 acgcttgttc ttgatcttta tagaaacctt tttctatcgc cagcctcata aaatgccccg
     1741 gggcatttac agaaataagt attccccccg gtattcttct atcatgattt cttgttctat
     1801 gagcagaaga gtaaaaaaca ttaacaccag ccttatatat ttttagccca tctttatcct
     1861 gaaatggtac gctttcagta taaataacat cctcttttat tggatagcac tctggcatat
     1921 gcagcgaaca aatatatttt tgtatgtcca ctaactcttc tgatggacga gcaaatgcaa
     1981 gttgtggatg aataaaataa atgagatgcg cactgacctc tcctcttctt gcgcgttctt
     2041 taaaacgaac tctttcatta cgcaaaaaat caaacaaatg accatcatca ttatatagtt
     2101 gggggctatt tacaatagat aaatggaaat caagcccttt aattttcttg cttgccaatt
     2161 taccaaatac acagggctga ttgcctgaca cccattttct tagtaaatct tcaattttat
     2221 ctttagaagt ttcgttaaag ataaatgaat gaatattatc aatatctttt ccaaatgaaa
     2281 aactggatga taattgtggt agtttattta tacgttgttg aagattcatg aaagctccca
     2341 caagacacct catatatttt ttatatactg aaactacatt atatttatta ccgacgtaaa
     2401 acgaaataat ttattatcgt taccgatatt ttaaaaaatc attatttgca cagtgttcga
     2461 gttgttagta tcactccttc ctgaaaatcc gctccattta ccataacgtc gcattgtacg
     2521 caccgccaaa acgcgttcgg tgcgatcacg gcagcagaca ggtaaaaatg gcaacaaacc
     2581 acccgaaaaa ctgccgcgat cgcacccgat aaattttaac cacatgcata gctatgcagc
     2641 catgtgaatc acgcaggaat tgccagccag agactgctga aacggatttt tttcatactt
     2701 tcggaaaggc agaagacgga accggagcgg aaaaacagaa attcatggga atgagcgcga
     2761 tctggcaata acggcaaaac acagcaacaa agacacacca gaatcgcgcc cgcatgcgtt
     2821 ttaacgcgtt cctgtgtttt cagcaagctc tgactgaagc gcgttcccgg tgcccccgaa
     2881 ccaaccatta tcccaaaaaa tgcaccggaa acgcgctgcc aacattttag cctgtctctg
     2941 gttaccatcc cagcgaaggg ccatccagtg tccgttcctg aatttccggc tggcgctccc
     3001 gctccaggga taacgcatat tcgcgctcct gtatcagccg ctcatccctt atctccagtt
     3061 ctcgctgtat aactggctca agcgttctgt ccgctcgctc aagtgctgca cctgctgact
     3121 caactgcatg actcgcccgt tcagcatcgc gttgtcccgt tgcgtaagcg aaaacatttt
     3181 ctgcaattcc acgaaggcgt tctcccattc gttcagccgc tgcatatagt cctgttgcag
     3241 ttgctctaat gcgttcagca aatgtctttc cagctccgtc atgctctgtc actcctgcac
     3301 cagtctcact gcgttcagtt tgccgttgtc cttcgtccag ttcgtttcgg ctttcatccc
     3361 cttcggcaac accagaaaac gtccgttgct gtcctcgtga taacgcacgc cccatgtctt
     3421 cgcgttcagc ttctccagac tctctttctg ctcgcggaga atattcatat tcgcgtttat
     3481 cttcatgccc tggtaccaca gcagactccc ggtcgtggca aacatcagta ctgagacgat
     3541 aatcagccac agccagcgtt tcacgctcat tttcagtacg gttttccagt gcgcgtccat
     3601 cgtatcgacg gtcgttttct gatacctctc cagcagttct ttcagggctg ttgtatggtc
     3661 gttgatagcg ttcctgatta aagattcgtt tgaggtcagt tccctgttta cagattcttc
     3721 gagcttcctg aactcgtcgt tcagcatttt ctctgtagat tcggctctct ctttcagctt
     3781 tttctcgaac tccgcgcccg tctgtaaaag attgctcata aaacgctcct ttcagtctga
     3841 tattcctccc gccgttcggg tctgcaatgc tgatactgct tcgcgtcacc ctgaccactt
     3901 caagccccgc ctctgtgagc gcctgaatca catcctgacg gccttttatc tctccggcat
     3961 ggtaaagcgc gtctatcccg cgtgtgacgc cctcagcaag cgcctgtttc gtttccggca
     4021 ggttatcagg aagggtcagc gtccggcggt tctccggggc gttggggtca tgcagaccgt
     4081 aatggtgatt caccagcgtc tgccaggcat caattctcgg cctgtctgcc cggtcgtagt
     4141 acggctggag gcgttttccg gtctgtagct ccatgttcgg aatgacaaaa ttcagctcaa
     4201 gccgtccctt gtcctggtgc tccacccaca ggatgctgta ctgatttttc tcgagaccgg
     4261 gcatcagtac acgctcaaag ctcgccatca ctttttcacg ccctccaggc ggcagctcct
     4321 tctccgcgaa cgacagaaca cctgacgtgt atttcttcgc aaatggcgtg gcatcgatga
     4381 gttcccggac ctcttccgga ttcccctgaa gcaccgttgc cccttcccgg ttacgctccc
     4441 ttcccagcag gtaatcaacc ggaccactgc caccaccttt tcccctggca tgaaacttaa
     4501 ctatcatccc gcgccccctg ttccctgacc gccagacgca gccggcgcag ctcatccccg
     4561 atggctatca gtgcggccac cacctgaacc cggtcaccgg aagaccactg cccgctgttc
     4621 accttcctcg ctgtctggtt aaggttattc ccgatagcgg ccagctgacg cagtaacggc
     4681 ggtgccagtg tcggcagctt cccggaacgg gcaacaggct cacccaggca gacccgccgc
     4741 atccatgccg ccagttgttt accctcacag cgttccagta aacgggcatg ctcatcatca
     4801 gtcacccgta tcgtgagcat cctctcgcgt ttcatcggta tcattacccc catgaacaga
     4861 aatccccctt acacggaggc atccagtgac taaacaggaa aaaaccgccc ttaacatggt
     4921 ccgctttatc agaagccaga cattaacgct gctggagaag ctcaacgaac tggacgcgga
     4981 tgaacaggca gacatctgtg aatcgcttca cgaccacgcc gatgagcttt accgcagttg
     5041 cctcgcacgc ttcggggatg acggtgaaaa ccgctgacac atgcagctcc cggagacggt
     5101 cacagcttgt ctgtgagcgg atgccgggag cagacaagcc cgccagggcg cgtcaggccg
     5161 ttgacctttg gggtgtcggg gcgaagccct gacccagtca cgtagcgata gcggagtgta
     5221 tactggctta atcattcggc atcagtgcgg attgtatgaa aagtgcgcca tgtcgggtgt
     5281 gaaatgccgc acagatgcgt aaggagaaaa tgccagttca ggcgctcttc cgcttcctcg
     5341 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggactctcc tttctcaaaa
     5401 acgctaaccc tgttatccac agaacccggg gataaaccag gaaagaacat gtgcgcaaaa
     5461 aacgaaaccc cggaaaaggc cgcgccggag gcgctttttc cataggctcc gcccccctga
     5521 cggacttcac aaaacagaca ctttttcggt gcatctgtgg gagccttgcg gctcaaccat
     5581 gaaatccgcc agcggtggcg aaacccgacg tggcttaaag atcatcaccg tttccggtga
     5641 atcgctccct cctgcgcccc cacgttcccg ccatatcgat taccggatac cttttccgcc
     5701 tttctccctt acgggacacg tggcgctttc tcatagctca cgtcttctgg tatctcgggg
     5761 cgacttaggc agttcgctcc gggctgggct gtatgcagga accccccgtt cagcccgact
     5821 gctgcgcctt ctccggtaac tgttctcttg ggtccaaccc ggaaaagaca cca
//
