LOCUS       NZ_OY754858             1547 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli isolate Reference plasmid pGPT21-004_5, complete
            sequence.
ACCESSION   NZ_OY754858
VERSION     NZ_OY754858.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA114565969
            Assembly: GCF_963575795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hendriksen,R., Thornval REBEKKA,N., S?rensen HOLM,L. and
            Lacy-Roberts,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2023) DTU National Food Institute, Global Capacity
            Building Group, Bygning 202 DK-2800 Kgs Lyngby, Denmark
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_963575795.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 23:59:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,626
            CDSs (total)                      :: 5,498
            Genes (coding)                    :: 5,206
            CDSs (with protein)               :: 5,206
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 292
            CDSs (without protein)            :: 292
            Pseudo Genes (ambiguous residues) :: 0 of 292
            Pseudo Genes (frameshifted)       :: 108 of 292
            Pseudo Genes (incomplete)         :: 168 of 292
            Pseudo Genes (internal stop)      :: 74 of 292
            Pseudo Genes (multiple problems)  :: 54 of 292
            Pseudo Genes (short protein)      :: 3 of 292
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754858.1.
            Reference for the DTU Genomic PT2021.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="Reference"
                     /db_xref="taxon:562"
                     /plasmid="pGPT21-004_5"
     gene            1..477
                     /locus_tag="AABJ60_RS28130"
     CDS             1..477
                     /locus_tag="AABJ60_RS28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1083..1164
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(1361..1469)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="100.0"
                     /evalue="9.09e-49"
     Typing          complement(710..968)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.402"
                     /coverage="99.0"
                     /evalue="2.35e-113"
ORIGIN
        1 atgtttattg attcagaaaa acgactgaaa caactttcag atgaggcaaa gaaaaacacc
       61 gaggatctcg aagaagcaaa gaagaattca aggtttacac aggtatcccc aaaaggttgg
      121 gaacgcgttc gagagctgct gaaggatagc caaggcatat cagcactgaa gctgtactca
      181 tttttagcgg agcatatcga tcctacgtgt ggcgctgtcg ttgcggatca gcaattccta
      241 gctgaaaaac ttggagttag cagaagcaca attattcggt ggctcaatta cttagaatca
      301 aaaaatgcat tagttagaat ccccgttgct ggtaaggttt gtgcgtatgc cctcgatcca
      361 catgaagtct ggaagggata caacactacg aaaaaccatg cagcgtttgt cactaaaaca
      421 ctggtcaaca aagacggtga tattcagcgc cgaatcatgg ccatgttttc aaattgagcc
      481 agcggcaggc ggacaatcag gggctacgtg ttaacgtttt gaccatgatt gtctatcctg
      541 cattgctctt ttgccgcctc aaaatccctt gcgtgttttt gctccccgat ccccagaacg
      601 accgcgccgc cacggttccg gcagcgcctt gagcgcaacc ggaaaccgtc tggggagaac
      661 cccagacccc catcgctgta acaccgacgg tctacgacca ccaaccagcc gaatagctgc
      721 ctgtgccgtg caaataggct ttgcaggggg ctgacgcggt tcctagcgac ctgaaagccc
      781 ttggttaggg gtatcggcgc gcctgacctc ctgagagcct ctgtaagcgt ttttcgcgcc
      841 ttccatgccc tgaccgggca tttaaacccg tttcgtgcgt ctgagagctt ctgagcgtgt
      901 ttgaagggct atctgacgac tcatccccga aagaacaaca acgggatcac cgacaacatg
      961 cgagaggtga tcccgctcgc cgcaagtcga tgaccgagcg tagcgagcga atcgacgagg
     1021 aagcggaaga gaaccggaag ttacattgag cacttacgca cggatgcggg gtgtcggggt
     1081 gaagccctga ccaggtgggg aatgtctgag tgcgcgtgcg cggtccgaca ttcccacatc
     1141 ctgtcccgat ttctgaggcg ttttaacggt gaacggacgg caaaaacatg atcgagctgt
     1201 tgaacgtgga gagcgcgaaa tcgggcaaaa tcacggctga cgaggcttgc agaggagtaa
     1261 gccagtatac actccgctat cgctacgtga ctgggtcagg gcttcgcccc gacaccccaa
     1321 aagggcgttg cgttgcgcgc aacacccttg cctagaatag atcttttacg ccgatttgta
     1381 acttgttgtt tttttagtgt gttttttgtt tacctgtcgc atccagggtg acacaacgtt
     1441 gtcgcatcct ggatgcgaca acacgcaaca aggtgttgca cagatgtgtc atgatggctt
     1501 agatttgaac caacgttagt gcatgggatt ttcagaggga aaaaatc
//
