LOCUS       NZ_OY754856             6675 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli isolate Reference plasmid pGPT21-004_3, complete
            sequence.
ACCESSION   NZ_OY754856
VERSION     NZ_OY754856.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA114565969
            Assembly: GCF_963575795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hendriksen,R., Thornval REBEKKA,N., S?rensen HOLM,L. and
            Lacy-Roberts,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2023) DTU National Food Institute, Global Capacity
            Building Group, Bygning 202 DK-2800 Kgs Lyngby, Denmark
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_963575795.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 23:59:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,626
            CDSs (total)                      :: 5,498
            Genes (coding)                    :: 5,206
            CDSs (with protein)               :: 5,206
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 292
            CDSs (without protein)            :: 292
            Pseudo Genes (ambiguous residues) :: 0 of 292
            Pseudo Genes (frameshifted)       :: 108 of 292
            Pseudo Genes (incomplete)         :: 168 of 292
            Pseudo Genes (internal stop)      :: 74 of 292
            Pseudo Genes (multiple problems)  :: 54 of 292
            Pseudo Genes (short protein)      :: 3 of 292
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754856.1.
            Reference for the DTU Genomic PT2021.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="Reference"
                     /db_xref="taxon:562"
                     /plasmid="pGPT21-004_3"
     gene            221..409
                     /locus_tag="AABJ60_RS28065"
     CDS             221..409
                     /locus_tag="AABJ60_RS28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177148.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLCG
                     ISHPAWCTFHTILTDAT"
                     /name="hypothetical protein"
     gene            complement(561..752)
                     /locus_tag="AABJ60_RS28070"
     CDS             complement(561..752)
                     /locus_tag="AABJ60_RS28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            820..1143
                     /locus_tag="AABJ60_RS28075"
     CDS             820..1143
                     /locus_tag="AABJ60_RS28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1352..>1786
                     /locus_tag="AABJ60_RS28080"
                     /pseudo=""
     CDS             1352..>1786
                     /locus_tag="AABJ60_RS28080"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1839..2381
                     /locus_tag="AABJ60_RS28085"
     CDS             1839..2381
                     /locus_tag="AABJ60_RS28085"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_235777954.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLEMLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTVG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2388..2621
                     /locus_tag="AABJ60_RS28090"
     CDS             2388..2621
                     /locus_tag="AABJ60_RS28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3111..3356)
                     /locus_tag="AABJ60_RS28095"
     CDS             complement(3111..3356)
                     /locus_tag="AABJ60_RS28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            3620..5713
                     /gene="cda"
                     /locus_tag="AABJ60_RS28100"
     CDS             3620..5713
                     /gene="cda"
                     /locus_tag="AABJ60_RS28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286654.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            5710..5973
                     /locus_tag="AABJ60_RS28105"
     CDS             5710..5973
                     /locus_tag="AABJ60_RS28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            6043..6189
                     /locus_tag="AABJ60_RS28110"
     CDS             6043..6189
                     /locus_tag="AABJ60_RS28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     BGC             1..6675
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          390..476
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="99.0"
                     /evalue="1.15e-36"
     Typing          6419..6603
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="98.0"
                     /evalue="2.18e-82"
     Typing          1134..2728
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.06"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caccggaaac ggtgatgatc tttaagccac gtcgggtttc gccaccgctg gcggatttca
       61 tggttgagcc tcacggctcc cacagatgca ccgaaaaagt gtctgttttg tgaagtccgt
      121 caggggggcg gagcctatgg aaaaagcgcc tccggcgcgg cctttttcgg ggttttcgtt
      181 tcttgctcac atgttctttc cggcgttatc ccctgattct gtggataaca ctatcaccgc
      241 ttttgagtgg gcagataccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag
      301 cgaggaagcg gaaaagcgcc tggacgagca ttttctcctt acgcatctgt gcggcatttc
      361 acacccggca tggtgcactt ttcataccat cctcactgat gccacatgat taagccagta
      421 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gctaaaaccc
      481 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg ctcacaggca agctgtgacc
      541 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat ccccgaagcg tgcgaggcag
      601 ctgcggtaaa gctcgtcagc gtggtcgtga agcgcttcgc agatgtcagc ctggtcgtcg
      661 gcgtccagtt cattcagttt ttccagcagg gtcagcgtct ggcttctgat gaagcgggcc
      721 atgttaaggg cggttttttc ctgtttagtc acagttctgt ttctccatgt gtggggagtc
      781 tgtcggaggt ttttaatgat accggtgaaa cgcgaaagga tgctcacgat acgggtcacg
      841 gatgaggaac atgcgcggct gctggcacgt tgtgagggaa aacaacttgc gtcatggatg
      901 cgtaaggtct gcctgggggc accgccatcg aaaacgtcag gtcttccgac actggcaccg
      961 ccgctgttgc gccagttcgc ttcggtcggc aataacctga atcagatagc ccggaagata
     1021 aacagtggac agtggtcggg gcatgaccgg gttcatgtgg tggctgcact gatggccatc
     1081 ggacgtgaac tgagtgagct gagggatgag gtccgtaaac agggagaacg ggatgatagt
     1141 taagtttcat gccaggggaa aaggcggtgg cagtggtccg gttgattacc tgctgggccg
     1201 ggagcgtaac cgggaagggg cgagagttct gcggggtgct cccgaagagg tgcgggaact
     1261 catcgatgcg acgccgtttg cgaaaaaata cacctcgggg gttctgtctt ttgcagagca
     1321 gaccctgcca ccgggagagc gtgaaagggt gatggagagc tttgagcggg tactgatgcc
     1381 cggtcttgaa aaaaatcagt acagcatcct gtgggtggag caccaggaca aggggcgtct
     1441 tgagctgaat tttgtcatcc cgaatatgga gctggcgagc ggaaaacggc tccagcccta
     1501 ttacgaccgg gcggaccgtc cgcgaatcaa tgcctggcag acactggtga atcaccatta
     1561 cgggctgcac gacccgaacg caccggagaa caggcggaca cttgtcaccc cgaataatct
     1621 gccgaaagcg aaacaggagg ccgcagaagc gattacgagg ggactggagg ctctttacca
     1681 tgccggagcg ctaaaaacgc gtcaggatgt cactgaggcg cttacagcgg caggttttga
     1741 ggttgtgagg acaaccagga gcagtatcag tatcgccgac ccggagggtg ggcgtaatct
     1801 caggctgaaa ggggcgctgt atgagcaatc ttttacaaat ggggacagac ttcgagaaga
     1861 aactgaaaga gcgtgccgtc tctacagaga acatgctgaa cagcgagttc aggaagctcg
     1921 aagcgtctgt agacagggaa ctgaacttaa acgggcagaa aatcagagac gctatcagcg
     1981 cacacacagc agcggtgaaa gaacaactgg agatgctgaa cagtgccgtg aacgcgcagt
     2041 tcagcgccac agagaacgaa cttgcccggc ggcaggaaga gctgctctgg aagctggtca
     2101 aagggcggat actgtacccg tcgctgacgg cgctttgcgt cacggtcggg atttttctgg
     2161 cgtcatgggg gctgattcag tggcaggaaa gccgtattgc ggcgaacatc ctggctatca
     2221 gggaccagga agagacgctg gcgaaactcc gggaaaaaac atggggtgtg acgtatcacg
     2281 agggcaggaa cgggaaattt ctggtgctgc cgtccggggt gaagggggaa aacaactgga
     2341 cggtggagaa gaagaacgca gtaagactgg tcagggagta agtgagtatg acagagctgg
     2401 aaatgcactt actgaacgcc ttagagcaat tgcagcagga ctatacgcag cggctgaaag
     2461 actgggagag cgccttcacg gatttgcagc atatgttcga gctgacacga caggagaacg
     2521 ggcaactgag ggaacagtgc gacgacttga gtcagcaggt gcagagatta gccgggcagg
     2581 tggaccgctt gagtcggtta tttatcacga acagcagata agggatgaac aggaaaggca
     2641 ggcacgcctg gaacgggagc gtgcgcggtc ctggcatgaa gagcaggaac gggaagcaca
     2701 ggtatggcgt ggcccgtcaa tggggatgtg acggtgagtt gttccgggag gttgccagat
     2761 ggtaccggaa cgcgttaaaa acgcatccgg gcgcgattct ggcgtgtcgt ttttgttgct
     2821 gtgttttgcc tctttgccag atcgcgctca ttcctcagcc tcatcccgcg atgtctgcgt
     2881 atgtcatcaa ctgaaaagat ggaaaaaaaa cagtgtcatg aggtttacgc aggcaaaacc
     2941 agcgttattc acatggctga atagctatgc atacggttaa aatttatcag gtgcgatcgc
     3001 ggcagctttt cgggtggttt gttgccattt ttacctgtca gcagccgtga tcgcgctgaa
     3061 cgcatttcag cggtgcgtac aataaggggt tatgttaaat aacccttagc ttagcggcgg
     3121 cgcagtggtt gccaggtgaa ggtgtcatcc gggtcctgtc cggcagtcag tgtggcttct
     3181 cgatttttgc tggccgttcg ttcatcaaga atggctttaa gtatccgtgc ccggaggcgt
     3241 cgctttgttt cctgcggcat tttgtccttt ttcgtcagcc ccatggcacg agcttcagcg
     3301 gcaatggctt ctgccagtcg gcgctcttcg gcttcggtca gtctatggcg tttcatggtg
     3361 gtctccgggt gtttctctgg tgtcataaca gtttaactgt tggctctctc cttatgctaa
     3421 tcagccttct ttcatgcctg ttgccattga gtttaagaat gattggttgt ttttcttgag
     3481 cgttttcaca aaaacgtaaa tccagtgggt tgacaggatt tacgaggtga cgtaactttt
     3541 tactgtacat aaaaccagtg gttttatgta cagtttgttg tttttaacgc attgttttaa
     3601 aagtcaaaga ggtgttttta tgagtgatta cgaaggtagt ggtccgacag aaggtattga
     3661 ttatgggcac tcgatggtcg tgtggccgtc aacaggactg atttcgggtg gtgatgtgaa
     3721 accaggaggc tcatcaggta tcgctccatc catgcctccg ggatgggggg attacagccc
     3781 acagggtatc gcacttgtac aaagtgttct ttttccggga attattcgcc ggattattct
     3841 ggataaggaa cttgaagagg gagactggtc gggatggtct gtcagtgtgc atagcccttg
     3901 gggaaacgag aaagtttccg ctgcacgaac agttcttgag aatggtttac gtggtggttt
     3961 gccagaaccg tctcgcccgg ctgctgtttc ttttgcccgt ctggagcctg cttccggaaa
     4021 tgagcaaaaa attattcgtc ttatggttac acagcaactg gaacaggtaa cggatattcc
     4081 ggccagccag ttaccagcag cgggtaataa tgtaccggtg aaatatcgtc tgatggacct
     4141 tatgcagaac ggtacgcaat atatggctat tatcggaggt attccgatga cagtgccggt
     4201 agttgatgcc gttccagttc cggaccggag tcgtccggga acaaatatta aagatgttta
     4261 cagtgcccct gtatcaccaa atctaccgga cctggtatta agtgtgggtc agatgaatac
     4321 tccggttctg tctaatcccg aaattcagga agaaggagtt attgctgaga caggtaatta
     4381 tgttgaggct ggttatacga tgtccagtaa taatcatgat gtcattgttc gttttcctga
     4441 tggcagtggt gtttctccgt tatatatttc aactgtagag attctggaca gtaatggtct
     4501 gagtcagcgt caggaagccg aaaataaagc aaaggatgat ttcagagtca agaaagaaga
     4561 agcggtagcc cgggctgaag ctgaaaaagc aaaagccgag ttatttagta aagcaggtgt
     4621 gaaccagcct cctgtatata cacaggaaat gatggaaagg gctaattcag taatgaatga
     4681 gcagggagct cttgttctga ataacactgc cagttctgta caactggcga tgaccggaac
     4741 tggtgtctgg actgctgctg gtgatattgc aggcaacata agcaagtttt tcagtaatgc
     4801 tctggagaaa gtaaccatac ccgaagtgag tcccctgctt atgcggattt ctcttggtgc
     4861 tctgtggttt cattcagaag aagctggagc aggaagtgat atcgtgccgg ggcgaaatct
     4921 ggaggcgatg ttttcgctga gtgctcagat gttggctgga cagggcgtgg tcattgaacc
     4981 tggtgcgacg agtgtaaatc tgcctgttcg tggacaattg ataaacagta acgggcaatt
     5041 agctctggat ttactgaaaa cagggaatga aagtatccca gctgcggttc ctgttcttaa
     5101 tgctgtccgt gatacagcaa caggactgga taaaatcacg ttaccagcag tagtaggcgc
     5161 accttcccgg acgattctgg ttaatccggt accacaacct tcggtgccaa cagatacagg
     5221 taatcatcaa cccgttccgg ttacaccagt gcatacagga acggaagtaa aaccggtcga
     5281 aatgccagta acgacgatta ctcctgtttc tgatgttggt ggactacgag attttattta
     5341 ctggcgtcct gatgctgctg ggactggtgt tgaagctgtt tatgtgatgc ttaatgatcc
     5401 tctggattca gggcgatttt cgcgtaaaca acttgataaa aaatataaac atgctggtga
     5461 ttttggtatt agtgatacaa aaaagaatcg tgaaactctt actaaattca gggatgctat
     5521 tgaggagcat ttatcggata aggatacagt agagaaagga acataccgaa gagaaaaagg
     5581 ttcaaaagtt tattttaatc ctaatacgat gaatgtggtt ataattaagt caaatggtga
     5641 gttcttatct gggtggaaaa taaatccaga tgcggataat ggtcgaattt atttagagac
     5701 aggtgaacta tgaacaagat ggcaatgatc gatttggcga aattattttt agcttcgaaa
     5761 attacagcaa ttgagttttc agagcgaatt tgtgttgaac ggagaagatt gtatggtgtt
     5821 aaggatttgt ctccgaatat attaaattgt ggggaagagt tgtttatggc tgctgagcga
     5881 tttgagcctg atgcagatag ggctgattat gaaattgatg ataatggact taaggttgag
     5941 gtccgatcta tcttggaaaa atttaaatta taatcatgag tgattttaat atctggcatt
     6001 taagtataaa tgtcagatat tcactcatga caaggagatg tcatgaaaaa aaagataggg
     6061 ggatctatga ttctcgttct tgctgttttg tgtctgactg cttgccaagc taactatgta
     6121 agggatgttc agggtggaac aattgctcca tcatcatctt cgaaactaat cggggtggcg
     6181 gttcagtgaa aaatattaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6241 gcctgtaaac gaaaaaaccc gccttgtggc gggtccttcc gtccgatcag aagtcttcga
     6301 cctcttctaa tcggcggtgg taactggctt ggaggagcac agtcaccaaa actgtctttt
     6361 cagtttagcc ttaactggtt cataacttca agagcaactc ctctaaatca gttaccaatg
     6421 gctgctgcca gtggcgttaa gtcgtgtctt ttccgggttg gccccaagag aacagttacc
     6481 ggagaaggtg cagcagtcgg gctgaacggg gggttcctgc atacagccca gcccggagcg
     6541 aactgcctaa gtcgccccga gataccagaa gacgtgagct atgagaaagc gccacgtgtc
     6601 ccgcaaggga gaaaggcgga aaaggtatcc ggtaatcgat atggcgggaa cgtgggggcg
     6661 caggagggag cgatt
//
