LOCUS       NZ_OY754855             6715 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli isolate Reference plasmid pGPT21-004_2, complete
            sequence.
ACCESSION   NZ_OY754855
VERSION     NZ_OY754855.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA114565969
            Assembly: GCF_963575795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hendriksen,R., Thornval REBEKKA,N., S?rensen HOLM,L. and
            Lacy-Roberts,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2023) DTU National Food Institute, Global Capacity
            Building Group, Bygning 202 DK-2800 Kgs Lyngby, Denmark
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_963575795.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 23:59:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,626
            CDSs (total)                      :: 5,498
            Genes (coding)                    :: 5,206
            CDSs (with protein)               :: 5,206
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 292
            CDSs (without protein)            :: 292
            Pseudo Genes (ambiguous residues) :: 0 of 292
            Pseudo Genes (frameshifted)       :: 108 of 292
            Pseudo Genes (incomplete)         :: 168 of 292
            Pseudo Genes (internal stop)      :: 74 of 292
            Pseudo Genes (multiple problems)  :: 54 of 292
            Pseudo Genes (short protein)      :: 3 of 292
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754855.1.
            Reference for the DTU Genomic PT2021.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="Reference"
                     /db_xref="taxon:562"
                     /plasmid="pGPT21-004_2"
     gene            join(6588..6715,1..1030)
                     /locus_tag="AABJ60_RS28015"
     CDS             join(6588..6715,1..1030)
                     /locus_tag="AABJ60_RS28015"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:1904047 -
                     S-adenosyl-L-methionine binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_100004725.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRGCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="DNA cytosine methyltransferase"
     gene            complement(1258..1416)
                     /locus_tag="AABJ60_RS28020"
     CDS             complement(1258..1416)
                     /locus_tag="AABJ60_RS28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001557254.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="hypothetical protein"
     gene            complement(2076..2267)
                     /locus_tag="AABJ60_RS28025"
     CDS             complement(2076..2267)
                     /locus_tag="AABJ60_RS28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165984.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGEDL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2332..2655
                     /locus_tag="AABJ60_RS28030"
     CDS             2332..2655
                     /locus_tag="AABJ60_RS28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955994.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2864..>3295
                     /locus_tag="AABJ60_RS28035"
                     /pseudo=""
     CDS             2864..>3295
                     /locus_tag="AABJ60_RS28035"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3333..3851
                     /locus_tag="AABJ60_RS28040"
     CDS             3333..3851
                     /locus_tag="AABJ60_RS28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001447511.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     gene            3858..4091
                     /locus_tag="AABJ60_RS28045"
     CDS             3858..4091
                     /locus_tag="AABJ60_RS28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001448503.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4466..4672)
                     /locus_tag="AABJ60_RS28050"
     CDS             complement(4466..4672)
                     /locus_tag="AABJ60_RS28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096539.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGKLMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(4665..5048)
                     /locus_tag="AABJ60_RS28055"
     CDS             complement(4665..5048)
                     /locus_tag="AABJ60_RS28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000065853.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            complement(5439..6500)
                     /locus_tag="AABJ60_RS28060"
     CDS             complement(5439..6500)
                     /locus_tag="AABJ60_RS28060"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="WP_000034299.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     NNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="HindVP family restriction endonuclease"
     Typing          1914..1998
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          complement(1345..1894)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.298"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2300..2649
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.162"
                     /coverage="99.0"
                     /evalue="1.96e-64"
     Typing          2646..4189
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.529"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccatccgata aaaaaactag atttgagcaa tgttgatgat gtggtgaggg agcttaaaga
       61 tatagacttc gatatgatta ttggagggcc tccttgccaa gacttcagtc atgcaggttt
      121 aagaattgaa ggtgctagag ctaacttaac tcgctcattt tcagaaatta taaaaagaat
      181 aaagccaaag tggtttgtga tggaaaatgt agatcgggca ttaagaagtg gtgcctattt
      241 agaagctcga ggaatcttta aagaatcagg gtatgggtta acagaaattg ttttggatgc
      301 cagtaagtgt ggtgttccgc aaaaaagaaa aagattattt gtaattggaa agcttgacgt
      361 gagggatggt tttatcctta acgaagtgat gtgcgggata tcgaaagata gtatgacagt
      421 tagaaattat cttggtgatt cattggggat tgagtattat tacagacatc cgagaaatta
      481 taacagaagg gctattttct ctattgatga acctgcgcca acagtaagag gggttaatag
      541 accgatacct gatggatatt tagggcatgc aggcgatcct gtcagtattt ctgagaatgt
      601 aaggccatta acaacctttg aaagagcaag attgcaaaca tttcctgaag actttaaatt
      661 taaaggtgca aaaacaaatt tggagcaaat gataggtaat gctgttccgg ttgagttagc
      721 aaaatatgtt gcggtgacaa tcatggagta tgaaaaaaaa caagtaaaag gtatttatga
      781 taaagaaggc tttagggcgt ggttgctgaa tgaaaagaaa ctaacaaaaa gaacctctag
      841 cgatattata tctagaggtt gcagaggtgt ttcatttttt gattcagagg gggtggattt
      901 ttataactgt gaaatagatg aaattattat gaaactggaa aggcttgaat cgtttgtcag
      961 gcttggggtt agcctgaagt ctcagttaag aagggctttt aaattatatt acgaatattg
     1021 tcgaaggtaa taaatacgaa tttatgtctc tcaggggagg gggaggggat aaaaagatca
     1081 aaggatcctc ttgtgatcct ttttttctgc gcgtaatctg ctgcctgtaa acaaaaaaac
     1141 caccgctacc aacggtggtt tgtttgccgg aatctagagc taccaactct ttcttccgaa
     1201 ggtaactggc ttggtggagc gcagatacca aatactgtcc ttctagtgta gccgcagtta
     1261 ggccaccact tcaagaactc ttaatatctc aatccacctt gtccagttac cagtggctgc
     1321 tgccagtggc gttaagtcgt gtctttccgg gttggactca agacgatagt taccggataa
     1381 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1441 ctacaccgaa ctgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1501 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1561 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1621 tgagcgtcga tttctgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcgg
     1681 cgcggccttt tgttgtcctt tgcctttttg ctcacatgtt ctttccggcc ttatcccctg
     1741 attctgtgga taaccgtatt accgcctttg agtgagccga ctccgctcgc cgcagccgaa
     1801 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     1861 tccttacgca tctgtgcggt atttcacacc gcatatggtg ctcttttctt ataatccgca
     1921 ctgatgccac atagttaagc cagtatacac tccgctatcg ctacgtgact ggttcagggc
     1981 ttcgccccga aaccccctga cgcgccctga cgggcttgtc tgctcccggc atccgctcac
     2041 aggcaagctg tgaccgtctc cgggagctgc atgtgtcaga ggtcttcacc gtcatcaccg
     2101 aaacgcgcga ggcagctgcg gtaaagctca tcggcgtggt cgtgaagaga ttcacaaata
     2161 tcggcctgtt catctgcgtc cagttcgttg agtttctcca gcagcgttaa tgtctggctt
     2221 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     2281 taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag gatgctcacg
     2341 atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg taaacaactg
     2401 gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg gaagctgccg
     2461 acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct gaatcagaca
     2521 gcgaggaagg taaacagcgg gcagtggtct tccggtgacc gggttcaggt ggtggccgca
     2581 ctgatggcca tcgagggaga gttgcgcagc ttacggcagg tcgtcaggga gcagggagcg
     2641 cgggatgatt gttaaatttc atgccagggg aaaaggcggt ggcagtggtc cggttgatta
     2701 cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta atccggaaga
     2761 agtcagggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg gtgttctgtc
     2821 gttcgcggag aaggaactgc cgccgggagg acgtgaaaaa gtgatggaga gctttgagcg
     2881 cgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg agcaccagga
     2941 caaggggcgt cttgagctga attttgtcat tccgaacatg gagctacaga ccggaaaacg
     3001 cctccagccg tactacgacc gagcagacag gccgagaatt gatgcctggc agacactggt
     3061 gaatcaccat tacgggctgc atgaccccaa cgccccggag aaccgccgga tactgactct
     3121 ctctgataac ctgccggaaa cgaaacaggc gcttgctgag agcgtcacac gcgggataga
     3181 cgctctttat catgtagggg agataaaagg ccgtcaggat gtgattcagg cgctcacaga
     3241 ggcggggctt gaagtggtca gggtgacgcg aagcagcatc agcattgcag acccgaacgg
     3301 cgggaagaat atcaggctga aaggagcgtt ttatgagcaa tcttttacag acgggcgcgg
     3361 agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg aacgacgagt
     3421 tcaggaagct cggcgagtct gtcagcgagg ctgtgacctc aaacgagacg aaaatcagag
     3481 acgctatagc cctgttcaca gcctcgacag agaaatcgct ggaaaaacac agggaagggg
     3541 tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg aatacgggca
     3601 tgatgttact ggggatagtc tttctcctgt ttaccgcgag tggcgggacg ctctggtatc
     3661 ttggcgggag gatacaggcg aacctggaag aaatcaggaa gcaggaagag acattgcaga
     3721 aactgaacgc gaagacatgg ggcgtggagt ttgtgcagga cgggagaagg aaatttctgg
     3781 tcattccgca agggatgtca gcagaggtga cgcactatca ggggaaggac tggatacaac
     3841 tgacggagtg atacggaatg acagagctgg aaacacattt gctgaacgcc ttagagcagc
     3901 tgcaacagga ctatatgcag cggctgaacg aatgggagag cgccttcgtg gaattgcaga
     3961 agatgttttc gcttacgcaa cgggacaacg cgatgctgaa cgagcgggtc atgcagttga
     4021 gtcagcaggt gcagcacttg agcgagcgga cagaacgctt gagccagtta tacagcgaga
     4081 actggagata agagaggaac ggctgataca ggagcgcgaa catgcgttat ccctggaacg
     4141 ggagcgtcag ccggaaatac aggaacgcat actggatggt ccttcgttgt cgctgtgatg
     4201 gcgaaactat aaaaaatggc aggttcagtg gattttgacg gactaatgta gtctgcacca
     4261 tctggttgca taggtattca tgcggttaaa atttatcagg cgcgatcgcg gcagtttttc
     4321 gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcaccgaac gcgttttagc
     4381 ggtgtgcgta acggatgtta tggtaaatac aatatgcagc cagtcttaga gacgaaaagt
     4441 ccgcccagaa gactgacttt tcagatcaac cttcagcatc taacttgtct atcgcttttt
     4501 tattgcgccg ataggtagcc attagtttac catcgcctga ttgtaagtca cgacctgctt
     4561 gctcagcata ggtatcgttt acagtcttag cgcctttttt ataggccttt ttaacatcat
     4621 caaatccggc ttggatgact tctttcggct tttcttgagg taatttagcc atgctgtgaa
     4681 tcctctataa ggttgattat ttttccaaca gataagacgg catcttctgt gtcgctgatt
     4741 ttatgattta tccaaccgtc ctctatcttt aatttgttca ctactcctaa tgtaaaaaca
     4801 atcttttttt caggaaagaa aattgcaaag gttctacttt tctctttcca atcagagggc
     4861 ggcgtcgaac ggaacgctac attcctcgat ttaagtatcg ctttgatttt actaattaat
     4921 tctttttttt gtttattgct gagatttttg acgaaccctt tgctgagatt gtctgtgtaa
     4981 tcatcaaaag aaatatcaac tggtttgttt ttttgttttt tatcagatag atgaacaatg
     5041 tttgacactg gatatcctgc tttttgatga atgaatactc agaagagtag cggaactgtc
     5101 ttgttagtca cagaggtaga gtatcaattc tacgatagat gggttcgaag atccggctga
     5161 gaccgttcag tacgcggtcg agatgttatg ttcaatgtaa tgaccgtcga aattgccttt
     5221 acgggcatga agcaaccagg agcaatcatt ggcaaaggta actagagttc aatgcatggt
     5281 aatcattttt gagcttgtga tgcatagagt tggttgctgt tccattgcgt ctcaaactcc
     5341 gtcaagatgc tcgccgtaaa agcgagcatg tctagtatgc ctgttccgct actgattttt
     5401 gatgggcgaa ttcagggctg gcgagccacc aagaaaaatt actttatttc atcaaacagg
     5461 tgagatgcag tataataaac gctttgatca aagcgtctct ctgggcttaa tttattttgc
     5521 ccgccattta aaattatttc ttttaatact gatggtggga ggatgggttt tgttcttctt
     5581 gggtgatcca tataagcttt tgttattttt ccgtttagcg agaactcttt atcactttga
     5641 tatccaaagt tcatctcagt ataaattcta ttgatgtttg tcttgttttt gctcgcaatt
     5701 tcatacacca ttctggcgag tctggctgat gatctcatta gtctgctggc agatgaattg
     5761 ttatatgagg atctatcaat aaaggttcga cacaaagcaa aatcactcca gatgaatatg
     5821 tcaaaggcat tttgatctaa tattgggctc ttgccttttg ttttccataa tggttgcatt
     5881 aaaaaaggtt tctggtaatc aagaaaattt tcttggaaag tgtttaggca atttaatata
     5941 ttttctttct ttgaaagaac ttcagtttca ttcccccagt gctgtatgct ttcgcatgtg
     6001 gattcaaata tttttcttat ttcatggaat ttatcgctgc aagagtgcat tatgccaagg
     6061 gcacaataag aagtgcttgc aggtcttatt acaatctctg aactccattc gctctcgttt
     6121 tctttatatg tcgtgctatc tggaagtacg gttaatttta tttctagtgc ttttaaaaaa
     6181 ctattgtttc cttttataac tagatctatt ccttttatgt catcataagc atactgttgg
     6241 tatggttcat attttttctc aaattgaaat tcaagatcac tattttttaa tgttgtgtta
     6301 aaaacgaagt caaaagatac ttctttggca tgcactttta aatccttatc taaataaaga
     6361 taaatcggat tcattttgtt gtctctcata taacatgcta gagaggtggg aaaagaactg
     6421 ttaaattggt tctttcccca cagatcgctc tctttcctgt tggtgttttt taaaccataa
     6481 agagattcta ttttgctcac tttttgctcc tgacgtcaat ttattgacgt ctatggtgtg
     6541 tttctatatc atgatgtgga gtgatatgat aatgagtttt ttatgttatg agaataattg
     6601 atctttttag tgggtgtggg gggttgtccc taggcttttt gaaggggggg tttgatgttg
     6661 taggtgctta tgatttctgg gatcctgcta ttgaatgtta cagagataat tttag
//
