LOCUS       NZ_OY754854             8119 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli isolate Reference plasmid pGPT21-004_1, complete
            sequence.
ACCESSION   NZ_OY754854
VERSION     NZ_OY754854.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA114565969
            Assembly: GCF_963575795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hendriksen,R., Thornval REBEKKA,N., S?rensen HOLM,L. and
            Lacy-Roberts,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2023) DTU National Food Institute, Global Capacity
            Building Group, Bygning 202 DK-2800 Kgs Lyngby, Denmark
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_963575795.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 23:59:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,626
            CDSs (total)                      :: 5,498
            Genes (coding)                    :: 5,206
            CDSs (with protein)               :: 5,206
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 292
            CDSs (without protein)            :: 292
            Pseudo Genes (ambiguous residues) :: 0 of 292
            Pseudo Genes (frameshifted)       :: 108 of 292
            Pseudo Genes (incomplete)         :: 168 of 292
            Pseudo Genes (internal stop)      :: 74 of 292
            Pseudo Genes (multiple problems)  :: 54 of 292
            Pseudo Genes (short protein)      :: 3 of 292
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754854.1.
            Reference for the DTU Genomic PT2021.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="Reference"
                     /db_xref="taxon:562"
                     /plasmid="pGPT21-004_1"
     gene            1..927
                     /locus_tag="AABJ60_RS27965"
     CDS             1..927
                     /locus_tag="AABJ60_RS27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271358.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNVSLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRGAVATS
                     ARTLKPWETLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1145..1420)
                     /locus_tag="AABJ60_RS27970"
     CDS             complement(1145..1420)
                     /locus_tag="AABJ60_RS27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091751.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPKPPAKPQNH"
                     /name="hypothetical protein"
     gene            1599..3818
                     /gene="mobQ"
                     /locus_tag="AABJ60_RS27975"
     CDS             1599..3818
                     /gene="mobQ"
                     /locus_tag="AABJ60_RS27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_024224537.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKEQDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSLGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            4051..5796
                     /gene="col"
                     /locus_tag="AABJ60_RS27980"
     CDS             4051..5796
                     /gene="col"
                     /locus_tag="AABJ60_RS27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_009451403.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAVPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSKQ
                     FNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHID
                     IHRGQ"
                     /name="col"
     gene            5799..6056
                     /locus_tag="AABJ60_RS27985"
     CDS             5799..6056
                     /locus_tag="AABJ60_RS27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_251706008.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPETVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            6118..6261
                     /locus_tag="AABJ60_RS27990"
     CDS             6118..6261
                     /locus_tag="AABJ60_RS27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            6726..6866
                     /locus_tag="AABJ60_RS27995"
     CDS             6726..6866
                     /locus_tag="AABJ60_RS27995"
                     /inference="COORDINATES: protein motif:HMM:NF020569.4"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WP_231237649.1"
                     /translation="MYEWDSQHGELEGYRASDGQHLGSFDPKTGNQLKGPDPKRNIKKY
                     L"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            6876..7133
                     /locus_tag="AABJ60_RS28000"
     CDS             6876..7133
                     /locus_tag="AABJ60_RS28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_000523347.1"
                     /translation="MGLKLHINWFDKTTEEFKGGEYSKDFGDDGSVIERLGMPLKDNIN
                     NGWFDVIAEWVPLLQPYFNHQIDISGNEYFVSFDYRDGDW"
                     /name="colicin E3-like toxin immunity protein"
     gene            7153..7419
                     /locus_tag="AABJ60_RS28005"
     CDS             7153..7419
                     /locus_tag="AABJ60_RS28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000861936.1"
                     /translation="MKVELKKSISDYTEAEFLELVKKICRAEGATEEDDNKLVREFERL
                     TEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            7481..7624
                     /locus_tag="AABJ60_RS28010"
     CDS             7481..7624
                     /locus_tag="AABJ60_RS28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..8119
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1425..1598)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(1599..1957)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..927
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.44"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1600..3815
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.917"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgaagaa aatttacccc accgtcccta tcacacggat
       61 gatctcgctt ttggtcttcg catctccggc aaagggcgtg cgcttcttgc gcggtacatc
      121 cagcagaacc agcctcatgc gcagttctgg ctggtttttg acgttgaccg cgagggggct
      181 gcgattgact ggagcgaccg gaacgccccc gcgccgaaca tcaccgtaaa aaatcccgtg
      241 aacggtcatg ctcacctgct ctatgcactc aacatcgccg tgagaaccgc gcctgatgcg
      301 tcggttaagg cactgaagta cgctgccgcg atagagcgtg cgctgtgtga gaaacttggt
      361 gcagatgtga actacagcgg actgatttgc aaaaatccgt tccacctgga atggctggtg
      421 atggagtggc gcgaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcctcagagc gtcgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggcctgt attctcacaa
      601 tggcttgatg ccgtgatcca gcgtgtcgaa atgtacaacg tatcgcttcc cgttccgctt
      661 tctccggcgg aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat tcaggctgca
      781 cgtggtcgca agggcgggaa aattggaggt gctaagtcta agcgtggtgc tgttgccaca
      841 tctgcgcgaa cgttgaaacc gtgggagact cttggaatta gccgcgcctg gtattaccaa
      901 ctgaaaaaac gaggtcttgt agagtagacc aaataaaacc tatatcagat aacagcgcct
      961 ttttggcgct tttttgagca gcttggtttg ttgctatttc cctcgttgaa tcccgcaatg
     1021 gcgcggcttt ccgcatgatt gaggttgtag cgctcgccgc agtctcatga ccgagcgtag
     1081 cgagcgaatg agcgaggaag cgcaaaggcg tccggtggtg catgtggctc ttacgcgccg
     1141 gggcttagtg gttctgcggt ttcgcaggtg gtttgggtag cttctccagc tcgttaatca
     1201 gcggttgtag tcggttcaca tccgcctgtc ttgtgacttc ctttcgcaga aactggagca
     1261 ggaacgcacg cagttgcgcc tcttccggcc tccgtaccct cgccagcatg gcagccccca
     1321 caatgacttt ttgcgccgtg tccaggctcc ggctcttcgc cttcaggcgc tgtaatctgg
     1381 cctcagcttc ggcaatcttc tgttcgagtg ttctgctcat tttgtgactc cgtgcgcggt
     1441 gaaaaatcgc attttatcgc gtcactggta gttgcaaaac tgaactggca taatgcacgg
     1501 cacatcacga agtgcgcact tatacaatct ccacttcgtt tcgattgtgt gcggtctgcg
     1561 acgctaaaag aaaacggcaa aaaggcatta cggcagaaat ggcgatttat catctcagca
     1621 tgaaaatcat ttcgcgaaaa aacggctaca gtgctgttgc ttctgctgcc taccgttccg
     1681 gctctgtcat acccgatgac cgtaccggat taatccacga ttacacccgt aaacgcggcg
     1741 ttgatgatgc ggtcattctc acccctgcga atgcaccgtc ctggtgtggt gaccgttccg
     1801 ttctctggaa tgcggtcgag aaagccgaac agcgccggaa ctcccagctg gcaagggaga
     1861 ttgaactcgc cattccccgt gagatttccc gcgaggccgc acgggagacc gttctcgctt
     1921 ttgtccggga aaactttgtc agtcggggca tgattgccga tgtggcgttc catcacatgg
     1981 accggaccaa tccccatgcg cacatcatgc tgaccacgag agctgtcggg gaaacgggat
     2041 tcgcaggaaa ggtcagggac tggaacgacc gggcactcgc cgagacgtgg cgcgcatcat
     2101 gggctgacca tgcgaacaga gcgcttgcga acgccggcta ccaggaagag atagaccatc
     2161 gttcatacga gcgtcaggga ctggagaaag cgccgggcct tcacctcgga aaggctgcct
     2221 gtgcgatgga aaaacgggga atggaaacag aacgcggtga gcagaaccgc ctgattaaca
     2281 gccttaacct ggaaatacag gtttcccgca caaggctggc agtcatccag gtacaggaga
     2341 acgtgcggaa acgggaactc agcgatgccg cacgtcgtgc agcggaagcc cttaacctga
     2401 ccattcccgc tgcgaatgcc tcagcggata ccctgcggga attcattgcc acgttgccgc
     2461 aggaatgcgg caacgcgtgg gagatgaccc cggagttcct ggcgatgagc gggaaggtga
     2521 acgacatcga acgtgagggg aatgcgctgc tgaaagagca ggccattctc gaaaaggaga
     2581 tgaccggact caaaaaagca cgccctgtcg cgtcccttct gtcagagatt cccctgatga
     2641 catgggctga accggaatac cgcaaaagac aactccgttt ctggaaactc gggaaacaga
     2701 ttgaatctct tcgccgcacc tacagggccg tgaaagaaca ggacattccc gcccgtcgtc
     2761 aggcctttga aacgcagtgg aatacgtgga ttgcgccggg aatggcagag ctgaaagaaa
     2821 aactgtcagc acgggaagcg gagcggcgca gggaggaagc cgaagcggaa gcgcgccgga
     2881 aggaacagga gcatgaggcg cggctgaaac gtcatgataa ccaccgtctg agccgtgaaa
     2941 cggcattagt cggggttatt acggagctgg ggcgtgccag agagccggga acgggcagga
     3001 taacccgcta catgatgctg agtaacagag ccggagaatt cacggtatgg ggtgatgagc
     3061 tggcgcatta cccccagagt gttcatgacc cggtgaatgt ttacctgtcg ctaggcgggg
     3121 ctgtgatggt ctcggatata cgtgagggaa tgccagaatc tcatgagacg atggcgcggc
     3181 ctgagcgtgt gagaatgtat tccggtgcga cggtccggca tgtactggaa cagatgcgcc
     3241 aggggtggcc ctcttacggt tttccggcgc tgccgcatca ctggccggat aatttttatt
     3301 tcagcgacga ccgcaggccc gtagcctctc cgctgccgtc agcgcaccgg gtggatatca
     3361 ccgctgatgc tgtaccggaa caactcatgc ctgttgtatt ttcgagagag ggaaacagca
     3421 ggacgctgaa tctgttgtta tgcaaagggc cggaggaaat gcttgtcggg tttgttcgcc
     3481 gggaaggagg gttttgcccc gttcttgtgc ttccgtcgcc ggattacagt catctgatag
     3541 tcagcaccat cacggagaac ggggtatgcc tggcaggtta cggagaagct ataaaccatg
     3601 atgaggatac tccgtaccca ccggaaccgc acctgatgca gttccggctc aaaggccatc
     3661 atgacaggct tttggctgct gtccacaaac cggaagagat gccggattat ctcttccgtc
     3721 aactcggttt taatcagacc tggcatgagt ggaagcggga cgaacagcac aggcaacaac
     3781 aacgccgccc cgggcacttc cggggcatga gtatgtgatg tccggggctg caccccggac
     3841 cccgccaaca catcacgggc cacaaaattt tttgtggccc gctctgcgtt ttctaagtgt
     3901 tatccctcct gatttctaaa aaattttcca cctgagcttg acagaaaaaa cgatgacggg
     3961 tactttttga tctgtacata aaaccagtgg ttttatgtac agtattaatc atgtaattaa
     4021 ttgttttaac gcttaaaaga gggaattttt atgagcggtg gcgatggacg cggccataac
     4081 acgggcgcgc atagcacaag tggtaacatt aatggtggcc cgaccgggct tggtgtaggt
     4141 ggtggtgctt ctgatggctc cggatggagt tcggaaaata acccgtgggg tggtggttcc
     4201 ggtagcggca ttcactgggg tggtggttcc ggtcatggta atggcggggg gaatggtaat
     4261 tccggtggtg gctcgggaac aggcggtaat ctgtcagcag tagctgtgcc agtggcattt
     4321 ggttttccgg cactttccac tccaggagct ggcggtctgg cggtcagtat ttcagcggga
     4381 gcattatcgg cagctattgc tgatattatg gctgccctga aaggaccgtt taaatttggt
     4441 ctttgggggg tggctttata tggtgtattg ccatcacaaa tagcgaaaga tgaccccaat
     4501 atgatgtcaa agattgtgac gtcattaccc gcagatgata ttactgaatc acctgtcagt
     4561 tcattacctc tcgataaggc aacagtaaac gtaaatgttc gtgttgttga tgatgtaaaa
     4621 gacgaacgac agaatatttc ggttgtttca ggtgttccga tgagtgttcc ggtggttgat
     4681 gcaaaaccta ccgaacgtcc gggtgttttt acggcatcaa ttccaggtgc acctgttctg
     4741 aatatttcag ttaataacag tacgccagca gtacagacat taagcccagg tgttacaaat
     4801 aatactgata aggatgttcg cccggcagga tttactcagg gtggtaatac cagggatgca
     4861 gttattcgat tcccgaagga cagcggtcat aatgccgtat atgtttcagt gagtgatgtt
     4921 cttagccctg accaggtaaa acaacgtcag gatgaagaaa atcgccgtca gcaggaatgg
     4981 gatgctacgc atccggttga agcggctgag cgaaattatg aacgcgcgcg tgcagagctg
     5041 aatcaggcaa atgaagatgt tgccagaaat caggagcgac aggctaaagc tgttcaggtt
     5101 tataattcgc gtaaaagcga acttgatgca gcgaataaga ctcttgctga tgcaatagct
     5161 gaaataaaac aatttgatcg atttgcccat gacccaatgt ctggcggtca cagaatgtgg
     5221 caaatggccg ggcttaaagc ccagcgggcg cagacggatg taaataataa gcaggctgca
     5281 tttgatgctg ctgcaaaaga gaagtcagat gctgatgctg cattaagtgc cgcgcaggag
     5341 cgccgcaaac agaaggaaaa taaagaaaag gacgctaagg ataaattaga taaggagagt
     5401 aaacggaata agccagggaa ggcgacaggt aaaggtaaac cagttggtga taaatggctg
     5461 gatgatgcag gtaaagattc aggagcgcca attccagatc gcattgctga taagttgcgt
     5521 gataaagaat ttaaaaactt cgacgatttt cggagaaaat tctgggaaga agtgtcgaaa
     5581 gatcctgagt taagtaagca atttaatcca ggtaataaaa aacgcttgag ccaaggatta
     5641 gctccacggg ctaggaataa agatactgtc ggtgggcgtc gctcatttga gcttcatcac
     5701 gacaagccaa tcagtcagga tggtggtgtt tatgatatgg acaacctccg tatcactacg
     5761 ccgaaacgcc atattgatat tcacagaggt cagtaaaaat ggaactaaaa aacagcatta
     5821 gtgattacac tgaaactgaa ttcaaaaaaa ttattgaaga catcatcaat tgtgaaggtg
     5881 atgaaaaaaa acaggatgat aacctcgagc attttataag tgttactgag catcctagtg
     5941 gttctgatct gatttattac ccagaaggta ataatgatgg tagccctgaa actgttatta
     6001 aagagattaa agaatggcga gctgctaacg gtaagtcagg atttaaacag ggctgaaata
     6061 tgaatgccag ttgtttaagg atgaatgact ggcattcttt cacaacaagg agtcgttatg
     6121 aaaaaaataa cagggattat tttattgctt cttgcagtca ttattctggc tgcatgtcag
     6181 gcaaactata tccgggatgt tcagggcggg accgtatctc cgtcatcaac agctgaagtg
     6241 accggattag caacgcagta acccgaaatc ctctttgaca aaaacaaagc gtgtcaggct
     6301 gattctgatg cgcttttttt gaaatgtcac aaaagttcca tgtgggagat gggatctaaa
     6361 atccctgtgc agaaccttcc atccggtggg agaaaacttg tcattttgac ctgttcgcca
     6421 ttcggggggg gcgcttggaa aacggaaatt ttcccacgct aagagaattg agttgaacga
     6481 gtggttacat ctaggccagc taactcactg aacgtaaagg ctgtacgaaa tgttccgacc
     6541 gtccgataac catttggctt acttccgtac atttccttgc gtagatgtac agaattgtac
     6601 gatttcggac accaaaaccg ctgttgtccc gccattctgg cgattcctga aagttatttg
     6661 actaaaaaat agtcttaacg tggtttattt tccgaattcc aagcggaccc cataaaggcc
     6721 gtaagattta tgaatgggac tcccagcacg gtgaacttga agggtatcgt gccagtgatg
     6781 gtcagcatct tggctcattc gatcctaaga ctggtaatca gttgaaagga cctgatccaa
     6841 aacgaaatat caaaaagtat ctttgagagg atgttatggg gcttaaatta catattaatt
     6901 ggtttgataa gacgaccgag gaatttaaag gtggtgagta ttcaaaagat ttcggtgatg
     6961 atggctcggt cattgaacgt cttggaatgc ctttaaaaga taatatcaat aatggttggt
     7021 ttgatgttat agctgaatgg gtacctttgc tacaaccata ctttaatcat caaattgata
     7081 tttccggtaa tgagtatttt gtttcatttg attatcgtga tggtgattgg tgatcaaata
     7141 ttctcaggag agatgaaggt ggagctaaag aaaagtatta gtgattatac cgaggctgaa
     7201 tttctggagc ttgtaaaaaa aatatgtaga gctgaaggtg ctactgaaga ggatgacaat
     7261 aaattagtga gagagtttga gcgattaact gagcacccag atggttcaga tctgatttat
     7321 tatcctcgcg atgacaggga agatagtcct gaagggattg tcaaggaaat taaagaatgg
     7381 cgagctgcta acggtaagtc aggatttaaa cagggctgaa atatgaatgc cggttgttta
     7441 tggatgaatg gctggcattc tttcacaaca aggagtcgtt atgaaaaaaa taacagggat
     7501 tattttattg cttcttgcag tcattattct gtctgcatgt caggcaaact atatccggga
     7561 tgttcagggc gggaccgtat ctccgtcatc aacagctgaa gtgaccggat tagcaacgca
     7621 gtaacccgaa atcctctttg acaaaaacaa agcgtgtcag gctgattctg atgcgctttt
     7681 tttttgaaat gtcacaaaaa ttccatgtgg gagatgggat ctaaaatcct cgtgcagaac
     7741 tttccatcca gggggagaaa acttgtcgtt ttgagccgtt cggtgttcag aacgcacgaa
     7801 accgatcgcg catcgctttc gtgaatagtt atgcaggccc ctgaaaacga ttctgacgcg
     7861 ttttttcggt tttgcctggt gttttcctgt ctttttgcgt tttttgcgtc agaacgcgtc
     7921 tgagggcgtt ttaaggggtg cgtacaacgg gagttatggt aaatggatcg ggttttcggg
     7981 aaggttcgac aggatttgcc gttgggtgta gtgtaagcga ctgaaaaaca aacgcccctg
     8041 aaattcgtgt ctctccgcca agattgtcac gagaatacag gggcgaggtc ggtcgataac
     8101 ctggaaggaa tcgtaacac
//
