LOCUS       NZ_OY754463             3598 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F95 plasmid pF95-9, complete sequence.
ACCESSION   NZ_OY754463
VERSION     NZ_OY754463.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542931
            Assembly: GCF_963575635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:14:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,304
            CDSs (total)                      :: 5,179
            Genes (coding)                    :: 4,956
            CDSs (with protein)               :: 4,956
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 74 of 223
            Pseudo Genes (incomplete)         :: 143 of 223
            Pseudo Genes (internal stop)      :: 52 of 223
            Pseudo Genes (multiple problems)  :: 41 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754463.1.
            Complete genomic sequence of E. coli strain F95.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F95"
                     /db_xref="taxon:562"
                     /plasmid="pF95-9"
     gene            complement(join(3485..3598,1..1404))
                     /locus_tag="R8904_RS26505"
     CDS             complement(join(3485..3598,1..1404))
                     /locus_tag="R8904_RS26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_000739598.1"
                     /translation="MKKNIVRQVLESYGPCISSELAERIKRQNPSMSSDAIRKMISRST
                     DIGKLPFLRFSHNRRFIYLKDDFGSFKFWRALKKCMREANSAYSHAILSVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYESVLKNLLSAKILRSVYIDGVGDCVLINNNIANDISIFNM
                     ANCESFFDKPIFELIKAWLRNLGIVAFNQIKTKYDGENNPVVGSFEWDITAPSYVSPLA
                     EYSSDGLIPGFVVCDFALGFNRNEITTDAADTFIRKVQMTKSSRTNQRIMFVLFARRFE
                     KNAFNKLRSEGVLAITIANAFGNKVDESLARLSKVIQGTLSIERHPDELLQMVKDLESI
                     SGENGNLRGYIFELFVSSQICNFYGYGNVRINKEYKINGKHAEADVVLETNDDIYIVEC
                     KNVKTLPSMEVSLWMKTRVPIINSYYKSNNPDNKNIHHRLWVTGNIYPKDINRLDKFKC
                     NNKKIDVDYLFGQSLDDFFYQKKQVFNLYRKVISPDYKKGQIPDFDL"
                     /name="NERD domain-containing protein"
     gene            complement(1837..2160)
                     /gene="mobA"
                     /locus_tag="R8904_RS26510"
                     /old_locus_tag="FGAF95_50570"
     CDS             complement(1837..2160)
                     /gene="mobA"
                     /locus_tag="R8904_RS26510"
                     /old_locus_tag="FGAF95_50570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2432..2626
                     /locus_tag="R8904_RS26515"
                     /old_locus_tag="FGAF95_50580"
     CDS             2432..2626
                     /locus_tag="R8904_RS26515"
                     /old_locus_tag="FGAF95_50580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041642.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAESGTGV"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3122..3271
                     /locus_tag="R8904_RS26520"
     CDS             3122..3271
                     /locus_tag="R8904_RS26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_317752569.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPKRHDLSPLAAATGNWNSRAMIM
                     RSVL"
                     /name="replication initiation protein"
     Typing          complement(2170..2394)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.167"
                     /coverage="100.0"
                     /evalue="4.85e-75"
     Typing          2672..3190
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.881"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(1841..2160)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="8.39e-60"
ORIGIN
        1 taaataatca acatcaatct tcttattatt acatttaaac ttgtccaatc ggtttatatc
       61 tttaggatat atattccccg taacccacaa acgatgatgt atatttttgt tatcagggtt
      121 atttgatttg tagtaggaat ttataattgg cactcttgtt ttcatccaaa gactaacctc
      181 catcgaaggt aaagtcttaa catttttgca ctcaacgata taaatatcat cgttagtttc
      241 caatacaaca tcagcttcgg catgtttacc gtttatttta tattctttat taatacgaac
      301 atttccataa ccatagaaat tacaaatttg agaggaaaca aaaagctcaa aaatataccc
      361 ccttaaattt ccattctctc cagagataga ctccagatct tttaccattt gaagtaactc
      421 atcaggatgt ctttcaatag ataaagttcc ctgaatcacc ttcgatagtc tggccaagct
      481 ctcatcgacc ttattaccaa acgcattagc tatagttata gccagaaccc cttcactcct
      541 tagcttgttg aaggcattct tctcaaaacg tctagcaaaa agaacaaaca taatcctctg
      601 gtttgtccta gaggacttgg tcatctgaac ttttcttatg aaggtatcag cagcatcggt
      661 ggtaatttca tttcgattaa accctagagc aaaatcacag acaacaaaac ctgggatgag
      721 tccatcgctg gaatattcag caagagggga cacataactt ggcgctgtta tatcccactc
      781 gaaagaacca accacaggat tgttctctcc atcatatttt gtttttattt gattaaaagc
      841 cacaatacct aaatttcgca accatgcctt aatcagttca aatattggct tatcaaaaaa
      901 agactcgcaa tttgccatat taaagatgct gatatcatta gctatattat tatttatcaa
      961 aacacaatca ccaacaccat caatatacac agatctaagt attttagctg aaagcagatt
     1021 ttttagaaca gattcataac ttaaatgctt ggcctgcttt atcggagagc cagacacaat
     1081 cccgaaatcc tttaccttca agtatccgcc attattaatt acggaaagta tagcgtgaga
     1141 atacgccgaa tttgcctcac gcatacattt tttgagcgcc cgccaaaatt tgaagctacc
     1201 aaagtcatct tttagataaa tgaatctcct gttatgacta aatcttaaaa atggcaactt
     1261 accaatatca gtgcttcgag agatcatttt acgtattgca tcagatgaca tagaaggatt
     1321 ctgacgctta atcctctcag caagttcaga agatatgcac ggaccataac tctcaagcac
     1381 ttgtctaaca atgttttttt tcataaattc atcttatttt tgtcacacta cgctatttat
     1441 aaaattacac tacgtgtaat atctatagat ccccaaaaaa aaacacaaag aaaaaaatca
     1501 taaaaatcaa atatttaaca aaagcaacat atcacaaaaa acacacctgc gacatttatc
     1561 cttaaaaatc aagatgttac ttttgtgaca tttcatcatc atatatcaac cgccctattt
     1621 accataatcc cttaattgta cgcaccactg aaacgcgctg aacgcgattc tgacgcagac
     1681 accgcaaaaa gagatgtttt accccggaaa aataggaagg atgcgtcagg atcgttttca
     1741 gctcgctgca tagttatgca tgaaagcgaa tggtgatcac tttgggagct tacggtgttc
     1801 ataccgtctg ttttcgacag ttttctcccc gggaagctaa tctgccataa gcctggataa
     1861 cagggcacgg tgatactccg taatagcgat cagcacctcc gcatactccc tgtccccgac
     1921 acgcttgccg tcgataaaga gttttttctg caacgccccc agtcgcatga cttctttcag
     1981 cacccggtca tcagtcagtg agttaacttt cttaccgaga gctgccgccc gtaaaaaacc
     2041 agaaacggtc ttgccgcatt cagcagccct ttttctgatt tcctggtctt cttctgccgt
     2101 cagacgaaca gggcgactga tagcccgcct gcgcgtatta cttccacttc gttttgtcat
     2161 aaaacatggc tccgtatctg acacggggtg tcggggcgaa gccctgacca gggtaattgt
     2221 aatagcgtgc atgcatgcgc ggtataacaa ttgcacatcc tgtcctgtct gcaagttcga
     2281 attaccaaca aagcactgct gttttttggc agtgacagtg ttgtctgact gaaatgtccg
     2341 tcggcagaat accggcggta agacggtcga aaagtggtac gcgatataat cacacgacgc
     2401 agaggacaga cagaacggag aaaacagaaa gatgaacaag cagcagcaaa ccgtactcaa
     2461 catggcggga ttcataaaaa gccagagcct gacgctgctc gaaaagctgg acgcactcga
     2521 tgccgacgaa caggccgcca tgtgtgagaa gctgcacgaa ctcgcggaag aactccagaa
     2581 cagcatacag acacgctttg aagcggaaag tggcaccggg gtataacagt gtatcctgct
     2641 ccagacagga tgcagaacgg tataccggta cgtaaggaga aaatgccaca taaggcgctc
     2701 ttccgcttcc tcgctcacta actcgctacg ctcggtcgtt cggctgcggc gagcggtatc
     2761 agctcactca aagacggtaa tgcggttatc cacagaatca ggggataaag ccaaaagaaa
     2821 catgtgagca aaaaacaaga acccgaaaaa ggccgcgccg atggcgtttt tccataggct
     2881 ccgcccccct gacgagcatc acaaaaatcg acgctcaagc cagaggtggc gaaacccgac
     2941 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3001 gaccctgccg tttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3061 tcatagctca cgctgttggt atctcggccc ggtgtaggtc gttcgctcca agctgggctg
     3121 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3181 gtccaacccg aaaagacacg acttatcgcc actggcagca gccactggta actggaatag
     3241 cagagcgatg ataatgcggt ccgtactata gagttcttga agtgatgccc caactacggc
     3301 tacacttaaa ggacaggatt tggtatctgc gctctgctga agccagttac ctaggttagg
     3361 taatctgcca ggattaacta accttcgaaa aaccaccttg ccgggtggtt ttttcgtttc
     3421 atggaggaag atacgctttt caagccgtca tcgtcaagaa atgcagagac tagaaaacgt
     3481 cattctataa gtcaaaatcg ggtatttgcc cttttttata atctggcgaa atgactttcc
     3541 tatataaatt aaacacttgt tttttttggt agaaaaaatc atccaaagac tgaccaaa
//
