LOCUS       NZ_OY754462             3904 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F95 plasmid pF95-8, complete sequence.
ACCESSION   NZ_OY754462
VERSION     NZ_OY754462.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542931
            Assembly: GCF_963575635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:14:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,304
            CDSs (total)                      :: 5,179
            Genes (coding)                    :: 4,956
            CDSs (with protein)               :: 4,956
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 74 of 223
            Pseudo Genes (incomplete)         :: 143 of 223
            Pseudo Genes (internal stop)      :: 52 of 223
            Pseudo Genes (multiple problems)  :: 41 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754462.1.
            Complete genomic sequence of E. coli strain F95.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F95"
                     /db_xref="taxon:562"
                     /plasmid="pF95-8"
     gene            138..584
                     /locus_tag="R8904_RS26490"
                     /old_locus_tag="FGAF95_50530"
     CDS             138..584
                     /locus_tag="R8904_RS26490"
                     /old_locus_tag="FGAF95_50530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_000184723.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWERRGKELLTKDSA"
                     /name="conjugal transfer protein TraD"
     gene            complement(845..1864)
                     /locus_tag="R8904_RS26495"
                     /old_locus_tag="FGAF95_50540"
     CDS             complement(845..1864)
                     /locus_tag="R8904_RS26495"
                     /old_locus_tag="FGAF95_50540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011017196.1"
                     /translation="MSAALQYFEENLPRRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKF"
                     /name="replication initiation protein"
     gene            complement(2320..3870)
                     /locus_tag="R8904_RS26500"
                     /old_locus_tag="FGAF95_50550"
     CDS             complement(2320..3870)
                     /locus_tag="R8904_RS26500"
                     /old_locus_tag="FGAF95_50550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001624483.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKASI
                     PQTAGERKAALVALRSRWADVQNEHLARYGHESRVDHRSLKEQGINRTPEVHLGPVQAA
                     SLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPELLLQYR
                     KVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARYLDKLGR
                     NVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHERNEFRNKL
                     QQAESEWDKENAAFKRTEGYKYVGDLDRYREREILAAASLENTRQKVAEEVSVARSQML
                     SLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALRIQRSWAREASRSNERDQDMER"
                     /name="MobA/MobL family protein"
     Typing          complement(1090..1864)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.613"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(2324..3870)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.629"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctcgacgaag gagagaaact actaagtgcg catccctgaa ttaatcaggg atgattttac
       61 ggtctcacac gcaggcagac aaggtgatat tatgcacaat aacaactcac attaacggca
      121 aaaatgcaga ggtaagaatg acaaatcgtg aagagtggtt aagtgcaaaa attgcctaca
      181 tcaacggact gaaatcaccg tcagagcagc agagattact tgtgttactg gcagagaaaa
      241 aaaaccgaac aacgacggat gaaaaaacgt tgtccgcact gatacgggca gaaaaaaccg
      301 cagagaaagc agccgcagca aaagccaggg taacggcaat catagcggca gaacggaagg
      361 cagccgcaag ggcggaacga aaggcgcggg atcatgaact ctacaaggcc gcaggcctca
      421 tgattgtggc aggactggtg gacagcaaaa ccggaaaacc aaagtttagt gctgccgaac
      481 tggtaggcgc actcgcagga atcgcagagc taccgcgcaa ccacccaaaa tggcaggaat
      541 gggaaagacg cgggaaggag ctactaacca aagacagtgc gtaagagcct ccgttcacgt
      601 tccgtttccc tggtgcttcc tcgctcattc gctcgctacg ctcggtcatg agactgcggc
      661 gagcacaacc acctcaatca tgcggtaagc cgcaccattg ctgggctgac aggagaaaac
      721 cgccggaaag cccgctgctc aaaaagacgc cgaaaggcgc tgttatctga tataggcttg
      781 ttcatgtccg cgaatttaac catccattaa caaaaacagg gggtaattgt tatttttctg
      841 ttaattaaaa tttaattatt tgtttcttgg ttaaccattc tccagaatta tccatccagc
      901 gtcgaatagt ccggctgcta acaccaaccg ccactgcaat atcttccgtt gacgctccca
      961 agatatacat atcaagcgcc ctacgacgtt tgtcgtcgtt agcagtccac cgaacagcgg
     1021 cgcttttttt ccctttgtcc gactgatttt ctgacgaatt ggccttcccg cccttgcgac
     1081 cgcgtgctgc ctgaatttct ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg
     1141 gcgtgaagtt cctgtgcgtg tatttcgcaa tgctcttgcc aatagcccga cattcaggcg
     1201 gagaaagcgg aacgggaagc gatgcgttgt acatttcgac acgctgaatc acggcatcaa
     1261 gccactgtga gaatgctggc cagccctgac gaatcgccct gtaagcccat ttgcgcgtca
     1321 tttcgaacag gtggcagttt cgccccatcc cgtaatgttt atcgatgcta cgacgcgctg
     1381 aggcgctcaa atcgagataa tcagccagtt catcgagggt ataggcttcc tcgcgccact
     1441 ccatcacctg ccattccagg tggaacggat ttttgcaaat cagtccgctg tagttcacat
     1501 cagcacaaag tttctcacac agcgaacgtt caaccgctgc ggcatatttc agcgccttaa
     1561 ccgacgcatc aggcgcggtt ctcacggcga tattgagtgc atagagcagg tgagcgtgtc
     1621 cgttcacagg atttttaacg gtgatgttcg gcgcgggggc gttccggtcg ctccagtcaa
     1681 tcgctgcccc ctcgcggtca acgtcaaaaa ccagccagaa ctgcgcatga ggctggttct
     1741 gctggatgta ccgcgcaagc agcgcacgcc ctttgccgga gatgcgaaga ccaaaagcga
     1801 gatcatccgt gtgataggga cggcggggta aattttcttc gaagtattga agcgcggcgc
     1861 tcatgtgtta cgattccttc caggttatcg accgacatcg cccctgtatt ttcgtgacag
     1921 tcttggcgga tagacacgaa tttcaggggc gtttgttttt cagtcgctta cactaccccc
     1981 aacgacaaaa cctgtcgagt cttcccgcaa acccgatcca tttaccataa ctcctgttgt
     2041 acgtaccccg taaaacgccc tcagtcgctt cctgacgcaa aaaacgcaaa aagacaggaa
     2101 aacaccaggc caaaccagaa aaacgcgtca gaatcgtttt cagaacgttg cataactatg
     2161 cacgaaagcg atgcgcgatc tgtttcgagc gttcagaagg gcgaacgggt caaaacagca
     2221 aactttctca tcagaaaacg gtgaacggga cgcggaattt tttcgcgttc cgtgaaacgt
     2281 tggcggggtt cggggcgcag tcccgatatt gctacatttt caacgctcca tatcttgatc
     2341 acgttcgtta ctacgtgatg cctccctggc ccaggatcgc tgtatacgca gtgccttttc
     2401 ctcctgttgc tgccgttcct gcgccatttc tgccagtaac tcatgtcgca tttgagcttt
     2461 ttcatctcca gatatactga gttcaggctc cagagataac atctggctac gggcaacgga
     2521 tacttcttca gcaacctttt gcctggtatt ctctaaagat gccgcggcca gaatctcacg
     2581 ttctctgtac ctgtccagat ccccaacgta cttatatccc tcggtacgct taaaagcagc
     2641 gttttctttg tcccactcgg attcggcttg ctgtagcttg ttcctgaatt cattcctttc
     2701 atgttcagcg gtttccagca cacttttttg ctccagaata cgcgcatcaa tctccctttt
     2761 gtcagccccc cgaaacaggc cacgaatccc attcaaagca ttgcgttgtt cctgtagctc
     2821 ccgcagctcc ctttgtgcgg aaacgacatt acgccccagt ttgtctaaat accgtgcccc
     2881 ttcatatatt cgcctgctcc attcggaaag agagtctttt tttgctttcg cattctgaag
     2941 cagcttatgt tctttgagcg cattgggatt ggcatcccct aaacgagcaa gccttgcttc
     3001 tccctgaatg accgttttca taaccttgcg gtactgaagc aataactccg gtgaacgggc
     3061 tgaacgaaca ggctcaactg ccttgatttt ttgcttttgc tcctgttcct gctgaatggc
     3121 atttgcggca tctctggcgg tcttgagttc ccgctcggca ttcctccgtt cctggattgc
     3181 aacaatctgc tcaccattga gactggcggc ctgaacgggg ccaagatgaa cttccggcgt
     3241 gcgattgata ccctgttctt tcagagaacg atgatctacg cgcgactcgt gaccgtatcg
     3301 cgcaaggtgc tcattctgca catctgccca gcgggaacgc agcgcaacaa gggcggcttt
     3361 tcgctctccg gctgtctggg gaatactcgc tttttttgcg cctccccgtt ctggtgattt
     3421 gctgttggca cgtttaaaaa attgctgcgg atcgcgttca ataccatcat taatccgctc
     3481 gcaaaacatg atatgcgcgt gtggctgttc ctttccgtcg attgcacctg gcggattgtg
     3541 gatcgcgtac tggtaggcat ggcgatcgcc aagttccttt tgcacgaaat cttcaacaag
     3601 ttctatccgt tgttcgcgtg tcagttcacg cggtaacgcg atctcaattt cacggtatgc
     3661 agtcccgttt tttcgttcaa attcatctgc ggctttccat agctctgcgg gatttttctt
     3721 cgcccatgat ggcatgttac ctgatgacga aaattccaga tcctcccgcg atttgtagct
     3781 cttatattcg ccggatctgg aaatgtaatc agcatgtttt aaggcactgc cttttgctcc
     3841 agctttgacg gtgcaatggt agcttgccat ctgcctttta gccctccgca ggagcgcaca
     3901 gttt
//
