LOCUS       NZ_OY754461             4091 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F95 plasmid pF95-7, complete sequence.
ACCESSION   NZ_OY754461
VERSION     NZ_OY754461.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542931
            Assembly: GCF_963575635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:14:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,304
            CDSs (total)                      :: 5,179
            Genes (coding)                    :: 4,956
            CDSs (with protein)               :: 4,956
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 74 of 223
            Pseudo Genes (incomplete)         :: 143 of 223
            Pseudo Genes (internal stop)      :: 52 of 223
            Pseudo Genes (multiple problems)  :: 41 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754461.1.
            Complete genomic sequence of E. coli strain F95.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F95"
                     /db_xref="taxon:562"
                     /plasmid="pF95-7"
     gene            complement(208..498)
                     /locus_tag="R8904_RS26475"
                     /old_locus_tag="FGAF95_50500"
     CDS             complement(208..498)
                     /locus_tag="R8904_RS26475"
                     /old_locus_tag="FGAF95_50500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_317752567.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     GETQLRAWLLHLLKTEVTRQADMSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="mobilization protein"
     gene            669..2816
                     /gene="mobQ"
                     /locus_tag="R8904_RS26480"
                     /old_locus_tag="FGAF95_50510"
     CDS             669..2816
                     /gene="mobQ"
                     /locus_tag="R8904_RS26480"
                     /old_locus_tag="FGAF95_50510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_317752568.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTRYLL
                     LHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQHVYSSATFEQ
                     LLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     QRDEYARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(3065..3766)
                     /locus_tag="R8904_RS26485"
                     /old_locus_tag="FGAF95_50520"
     CDS             complement(3065..3766)
                     /locus_tag="R8904_RS26485"
                     /old_locus_tag="FGAF95_50520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(503..653)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.81"
                     /coverage="85.0"
                     /evalue="3.53e-55"
     Typing          complement(670..1024)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3067..3766)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.301"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          628..2813
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.588"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caacaggtta caaaaaagca ggttcgcaag ctttagcaaa accccgctca accctgtgga
       61 aatccagtat tcatgcgggt tcagcgcata tcacctgtcg aaaaaccgtg cgtatgtgct
      121 caatctggcg acggattcac ttccgcttcc tcgcccactc gctcgctaac gctcggtcgc
      181 tggggctgcg gcgagcggac ggtgcattca cgcctgttgg tcgttttctg acacttcttt
      241 ggggactggt tttggtaacg cgttcagttc gtcaatcaac ggctgtatcc tgctcatatc
      301 agcctgccgt gttacctctg tcttcaggag atgaagcaac caggctcgta gttgtgtttc
      361 cccaggcttc ctgacctttg ccagcaatgc cgctcccaca atgaccttct gcgctgtgtc
      421 caggctcctg ctcttcgcct tcagacgctg caatctggcc tcagcatcgg caatcttctg
      481 ttcgagtgtt ctactcattc cgtaactccg tgcgcggtga aaaatcgcat tttagcgcgt
      541 cactggtagt ttaaaaactg aactggcata atgcacagca aatcacgaag tgcgcactta
      601 tacaaactct ccttcgtcga gtttgtgtgc gccctgcggg gctaaaagaa aacggcaaca
      661 acgacacgat ggcaatttat catctcagca tgaagattct ttccagaagc aagggctaca
      721 gcgccgttgc ttctgctgcc tatcgtgccg gagaaaaaat actggatgag cgcaccggag
      781 tgattcacga ctacacccgc aaaaacggtg tagccagcgc agtgattctg acgccagcaa
      841 acgccccagc atggtgtgcg aaccgcgccg aactctggaa tgctgttgaa aaagccgagc
      901 ggcggaaaaa ctcacaactt gcgcgggagt tcgaactcgc catcccccgt gaactggcgc
      961 aggatgccgc acgggaaaca gttctcaact tcgtaaggga aaactttgtc agtcgcggca
     1021 tgatcgccga cgtggccttt cacaatctgg gcgggtcaaa cccacacgcg cacatcatgc
     1081 tatccatgag ggcaatcacg cctgacggct tcggggagaa agtccgtgag tggaatgact
     1141 ggactcacgc cgaaacatgg cggggatcat gggcagacca tgcgaacagg gcgctggcga
     1201 acgccggtta tcaggaagag atagaccatc gttcatacga gcgacagggg gttgagaaaa
     1261 cgcctggcat tcacctcgga aagtcggcct gtgcgatgga aaagtggggc atcgaaacag
     1321 aacgcggcga acagaatcgc ctgattaaca ggcttaacct ggaaatacag gtttcccgcg
     1381 acgaactcag gaacagtgag ctgacagagc ccgcacgccg tatggctgac cttctgaata
     1441 ttgagcttcc tgacaacgca acttcgtaca tactgcatga catcatcgaa gcgttgccga
     1501 cggacagtaa cgcagcatgg aaactcacca gtaaagccat gtcgatgatg gcagatatgc
     1561 aggcgacgcg cagacgctgg gaagatctga acaccgaacg aaaggaagcg atgagccatg
     1621 ctgccagcct gaaaaaatca tcgccgtttt cgtccggttt ttcccgtatt cccctgatgc
     1681 aatgggttgc accggaatac ggccgcgaac aggaaaaaat ccagtcgttg agccagcgca
     1741 tggaaaaatt gcggcagcat cactggaagg ttgagaaaca ggacattccc gcgagtcaaa
     1801 aagcatttga aacacagtgg cagcagtggg gtgcatctgg ccttgcagat ctccgagaac
     1861 agttgcgtaa acgtgaagag gaattgcgcc ggaaggagca ggaggaaaca gaacgtcgtc
     1921 gggcggagca gctcaggaga aatgacaatg ccgttatgga ttcaggggta ctgaaagcag
     1981 tcgttacggg gtatggcgag gcccccatgc caggtaacgg ggaaatgacc cgttaccttc
     2041 tgcttcacaa ccggaacgga gaatatacgc tctgggggaa cgaactggag aaataccgcg
     2101 ccagaatttt tgagagtgtt gatttaatgc gtgacaggag tggttatatc tgtgagcgaa
     2161 gcgaaatcgg tcaacgtcca ccactgcaac atgtttattc ctctgcgacc tttgagcaac
     2221 tgctgacgca ggtttgccag acatggccgc aacatacccg cgatctccgc cagccgaaga
     2281 catggccgga gtctttctgt cttggagaag accgccagcc agccatgcca tcactggcgg
     2341 caaggaaggt ggattttacg cagggacgtc tgccaccgac tctgatgccg gtaatgagtt
     2401 ctgtcgaccg tgaaacccgc cagcttcagc ttttgctggt catgggtgtg gatgactcta
     2461 tgggcggtgt tgtccggctt aatggtacgc tgtatcccgc gtttgccgtc cccacagccg
     2521 acaacagcca gctggtaatc aatgcactga ccgacaaagg tttacgcttt gcaggctatg
     2581 gtgaagcggt aaaccacgat gctgacagtc cggcacgtcc tgcgccggaa cttatgcagt
     2641 ttcatctgaa aacctggagc gaaccgcttt ttgctatcgt caacacgcca gaaaaacagc
     2701 cggatcatct gttccgctcg ctgggctttg agcgcacctg ggatgaatgg caacgtgatg
     2761 aatatgcccg aacacacgcc accgagagga gacatgatcg cggttggtca cagtgaatgt
     2821 gaccaatatt ttgttttgtt taacatcgtg ttatgttcgt cgatgttcat cgacagggat
     2881 gaatgcaaga agcacacaca taacacaaat caactttata gttttcccgc ccccgaaaaa
     2941 ttgggggttc ttttttcact gtgccagagc gtttatgacg caatttttta cttcaccagt
     3001 accttcgcat gccaaacaca aaaaaaacgc gtcagagagc gttttagagg ccttaaaatc
     3061 gattctagat cgtcatcttg atctgtttgt cttccttgaa gcgaaaatgc agggctacaa
     3121 ccgtgcgccc cttcttgatc gtctcaagcg ttacagccaa gtcgcttttt tgattcagtt
     3181 cgtcaacgca aggtcttaat aattgctggt tcagagactt atatgtttga tatttatgct
     3241 ctatatcaag cattgagcga aaatcatcga gggcgataat acgttcccct gtgctgcgga
     3301 attgctggag aagttcgtat attcttatgc tgtaacttct tgataaattg gagatgttct
     3361 taattacaac tctagtaaat tgccccttca actgtgtcag ataaggcatg accgcatctg
     3421 aaaaagtgat ttgggctttg ccctcacctt tagcgtactg tgcccgatac tggatccagc
     3481 ggaactcctc gcgcttctca tcgtccttaa ggatgatcga cctgtcccac aaacgatcaa
     3541 ttgcttcctg tagctgaacc tcggcatttt tccgccccat atcagggaat gagtcgaaat
     3601 actcttcggc ggtaacagtc atggtctttt gcaattcagt ggattctgca tcagaacccg
     3661 acttcaatcg accaatgcaa agtaacaaga atcgctgttc ctgaagagtc aatttatagc
     3721 tggcctcaac cagtgcattg gatttgtagg caatcagatc cgacattgta aatccctcaa
     3781 cctaaggtaa tgagtaaaat ataaacattc ctttaggtaa aggcaatatg atttaatttt
     3841 aatatccctt ttcctgtgaa tattacctta aatcatattg cctttacctt aaatcatatt
     3901 gcctatccac cttaaatcat attgccttta ccttaaatta tattacctat agtcttctaa
     3961 actcttgaat ttcataagaa aattcgaccc taaaacaagt attaaaaaca agatcttaaa
     4021 aacaagatct aaaaacttaa cggcgagcct gtggaaaact tggggtgttc aaaacaaaaa
     4081 atcatgaaaa a
//
