LOCUS       NZ_OY754460             5873 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F95 plasmid pF95-6, complete sequence.
ACCESSION   NZ_OY754460
VERSION     NZ_OY754460.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542931
            Assembly: GCF_963575635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:14:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,304
            CDSs (total)                      :: 5,179
            Genes (coding)                    :: 4,956
            CDSs (with protein)               :: 4,956
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 74 of 223
            Pseudo Genes (incomplete)         :: 143 of 223
            Pseudo Genes (internal stop)      :: 52 of 223
            Pseudo Genes (multiple problems)  :: 41 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754460.1.
            Complete genomic sequence of E. coli strain F95.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F95"
                     /db_xref="taxon:562"
                     /plasmid="pF95-6"
     gene            complement(1..1053)
                     /locus_tag="R8904_RS26440"
                     /old_locus_tag="FGAF95_50430"
     CDS             complement(1..1053)
                     /locus_tag="R8904_RS26440"
                     /old_locus_tag="FGAF95_50430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021517772.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGNGGYSHPEGISTTWHSESKLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIDNSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGVPVDDASMFFNSFQPRKFE
                     NNPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(1773..2006)
                     /locus_tag="R8904_RS26445"
                     /old_locus_tag="FGAF95_50450"
     CDS             complement(1773..2006)
                     /locus_tag="R8904_RS26445"
                     /old_locus_tag="FGAF95_50450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021520366.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2013..2543)
                     /locus_tag="R8904_RS26450"
                     /old_locus_tag="FGAF95_50460"
     CDS             complement(2013..2543)
                     /locus_tag="R8904_RS26450"
                     /old_locus_tag="FGAF95_50460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2581..3279)
                     /locus_tag="R8904_RS26455"
                     /pseudo=""
     CDS             complement(<2581..3279)
                     /locus_tag="R8904_RS26455"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3221..3544)
                     /locus_tag="R8904_RS26460"
                     /old_locus_tag="FGAF95_50470"
     CDS             complement(3221..3544)
                     /locus_tag="R8904_RS26460"
                     /old_locus_tag="FGAF95_50470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024188420.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAAWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALIAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3610..3801
                     /locus_tag="R8904_RS26465"
                     /old_locus_tag="FGAF95_50480"
     CDS             3610..3801
                     /locus_tag="R8904_RS26465"
                     /old_locus_tag="FGAF95_50480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021539356.1"
                     /translation="MTKQEKTALNMVRFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5006..5872)
                     /locus_tag="R8904_RS26470"
                     /old_locus_tag="FGAF95_50490"
     CDS             complement(5006..5872)
                     /locus_tag="R8904_RS26470"
                     /old_locus_tag="FGAF95_50490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     Typing          complement(3896..3977)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          complement(4434..4621)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(1670..3231)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.232"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaatcatcc tctttctcat aaagggggtt gttttcaaat ttccttggtt gaaaggagtt
       61 aaaaaacata gatgcatcat ctacaggaac accaataaac tctccataat aaggatcatc
      121 tataggaaac tcttctaatg aaacataaaa caatacattc cagttgaaga tcattggatc
      181 actattatct atttcatgga gcaatctttc atccttagtt aattctcctg gaattaatac
      241 atcagtatga taaaaaccgc tgtaataaga actattaccg gtgtgcactg ggcatccaaa
      301 acgatccaat ttagattctg aatgccatgt tgtagaaatc ccttccggat gtgaataccc
      361 accgttacca acagactgaa tagtcatgtt acgaatatcc gctaacgctt gttcttgatc
      421 tttatagaaa cctttttcta tcgccagcct cataaaatgc cccggggcat ttacagaaat
      481 aagtattccc cccggtattc ttctatcatg atttcttgtt ctatgagcag aagagtaaaa
      541 aacattaaca ccagccttat atatttttag cccatcttta tcctgaaatg gtacgctttc
      601 agtataaata acatcctctt ttattggata gcactctggc atatgcagcg aacaaatata
      661 tttttgtatg tccactaact cttctgatgg acgagcaaat gcaagttgtg gatgaataaa
      721 ataaatgaga tgcgcactga cctctcctct tcttgcgcgt tctttaaaac gaactctttc
      781 attacgcaaa aaatcaaaca aatgaccatc atcattatat agttgggggc tatttacaat
      841 agataaatgg aaatcaagcc ctttaatttt cttgcttgcc aatttaccaa atacacaggg
      901 ctgattgcct gacacccatt ttcttagtaa atcttcaatt ttatctttag aagtttcgtt
      961 aaagataaat gaatgaatat tatcaatatc ttttccaaat gaaaaactgg atgataattg
     1021 tggtagttta tttatacgtt gttgaagatt catgaaagct cccacaagac acctcatata
     1081 ttttttatat actgaaacta cattatattt attaccgacg taaaacgaaa taatttatta
     1141 tcgttaccga tattttaaaa aatcattatt tgcacagtgt tcgagttgtt agtatcactc
     1201 cttcctgaaa atccgctcca tttaccataa cgtcgcattg tacgcaccgc caaaacgcgt
     1261 tcggtgcgat cacggcagca gacaggtaaa aatggcaaca aaccacccga aaaactgccg
     1321 cgatcgcacc cgataaattt taaccacatg catagctatg cagccatgtg aatcacgcag
     1381 gaattgccag ccagagactg ctgaaacgga tttttttcat actttcggaa aggcagaaga
     1441 cggaaccgga gcggaaaaac agaaattcat gggaatgagc gcgatctggc aataacggca
     1501 aaacacagca acaaagacac accagaatcg cgcccgcatg cgttttaacg cgttcctgtg
     1561 ttttcagcaa gctctgactg aagcgcgttc ccggtgcccc cgaaccaacc attatcccaa
     1621 aaaatgcacc ggaaacgcgc tgccaacatt ttagcctgtc tctggttacc atcccagcga
     1681 agggccatcc agtgtccgtt cctgaatttc cggctggcgc tcccgctcca gggataacgc
     1741 atattcgcgc tcctgtatca gccgctcatc ccttatctcc agttctcgct gtataactgg
     1801 ctcaagcgtt ctgtccgctc gctcaagtgc tgcacctgct gactcaactg catgactcgc
     1861 ccgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa ttccacgaag
     1921 gcgttctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc taatgcgttc
     1981 agcaaatgtc tttccagctc cgtcatgctc tgtcactcct gcaccagtct cactgcgttc
     2041 agtttgccgt tgtccttcgt ccagttcgtt tcggctttca tccccttcgg caacaccaga
     2101 aaacgtccgt tgctgtcctc gtgataacgc acgccccatg tcttcgcgtt cagcttctcc
     2161 agactctctt tctgctcgcg gagaatattc atattcgcgt ttatcttcat gccctggtac
     2221 cacagcagac tcccggtcgt ggcaaacatc agtactgaga cgataatcag ccacagccag
     2281 cgtttcacgc tcattttcag tacggttttc cagtgcgcgt ccatcgtatc gacggtcgtt
     2341 ttctgatacc tctccagcag ttctttcagg gctgttgtat ggtcgttgat agcgttcctg
     2401 attaaagatt cgtttgaggt cagttccctg tttacagatt cttcgagctt cctgaactcg
     2461 tcgttcagca ttttctctgt agattcggct ctctctttca gctttttctc gaactccgcg
     2521 cccgtctgta aaagattgct cataaaacgc tcctttcagt ctgatattcc tcccgccgtt
     2581 cgggtctgca atgctgatac tgcttcgcgt caccctgacc acttcaagcc ccgcctctgt
     2641 gagcgcctga atcacatcct gacggccttt tatctctccg gcatggtaaa gcgcgtctat
     2701 cccgcgtgtg acgccctcag caagcgcctg tttcgtttcc ggcaggttat caggaagggt
     2761 cagcgtccgg cggttctccg gggcgttggg gtcatgcaga ccgtaatggt gattcaccag
     2821 cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg tagtacggct ggaggcgttt
     2881 tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc ccttgtcctg
     2941 gtgctccacc cacaggatgc tgtactgatt tttctcgaga ccgggcatca gtacacgctc
     3001 aaagctcgcc atcacttttt cacgccctcc cggcggcagc tccttctccg cgaacgacag
     3061 aacacctgac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc ggacctcttc
     3121 cggattcccc tgaagcaccg ttgccccttc ccggttacgc tcccttccca gcaggtaatc
     3181 aaccggacca ctgccaccac cttttcccct ggcatgaaac ttaactatca tcccgcgccc
     3241 cctgttccct gaccgccaga cgcagccggc gcagctcatc cccgatggct atcagtgcgg
     3301 ccaccacctg aacccggtca ccggaagacc actgcccgct gttcaccttc ctcgctgtct
     3361 ggttaaggtt attcccgata gcggccagct gacgcagtaa cggcggtgcc agtgtcggca
     3421 gcttcccgga acgggcaaca ggctcaccca ggcagacccg ccgcatccat gccgccagtt
     3481 gtttaccctc acagcgttcc agtaaacggg catgctcatc atcagtcacc cgtatcgtga
     3541 gcatcctctc gcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg
     3601 aggcatccag tgactaaaca ggaaaaaacc gcccttaaca tggtccgctt tatcagaagc
     3661 cagacattaa cgctgctgga gaagctcaac gaactggacg cggatgaaca ggcagacatc
     3721 tgtgaatcgc ttcacgacca cgccgatgag ctttaccgca gttgcctcgc acgcttcggg
     3781 gatgacggtg aaaaccgctg acacatgcag ctcccggaga cggtcacagc ttgtctgtga
     3841 gcggatgccg ggagcagaca agcccgccag ggcgcgtcag gccgttgacc tttggggtgt
     3901 cggggcgaag ccctgaccca gtcacgtagc gatagcggag tgtatactgg cttaatcatt
     3961 cggcatcagt gcggattgta tgaaaagtgc gccatgtcgg gtgtgaaatg ccgcacagat
     4021 gcgtaaggag aaaatgccag ttcaggcgct cttccgcttc ctcgctcact gactcgctac
     4081 gctcggtcgt tcggctgcgg cgagcggact ctcctttctc aaaaacgcta accctgttat
     4141 ccacagaacc cggggataaa ccaggaaaga acatgtgcgc aaaaaacgaa accccggaaa
     4201 aggccgcgcc ggaggcgctt tttccatagg ctccgccccc ctgacggact tcacaaaaca
     4261 gacacttttt cggtgcatct gtgggagcct tgcggctcaa ccatgaaatc cgccagcggt
     4321 ggcgaaaccc gacgtggctt aaagatcatc accgtttccg gtgaatcgct ccctcctgcg
     4381 cccccacgtt cccgccatat cgattaccgg ataccttttc cgcctttctc ccttacggga
     4441 cacgtggcgc tttctcatag ctcacgtctt ctggtatctc ggggcgactt aggcagttcg
     4501 ctccgggctg ggctgtatgc aggaaccccc cgttcagccc gactgctgcg ccttctccgg
     4561 taactgttct cttgggtcca acccggaaaa gacaccacaa agcgccactg gcagcagcca
     4621 ttggtaaaag tcatgtgcga gatatttaga gagtacttga agtggctgcc ggagtgcggc
     4681 tacactagaa gaacaatttt ggtgactcgt ctcgcataag acagttacct ggttaagcag
     4741 tctgcaagct gacttaacct tcgatcaaac cgccctgcaa ggcggttttt tcgtttacag
     4801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaggat cctttgatct ttttgttaca
     4861 aacgtctcta tttgactccc ctgagagcgt tttagcgcgc ctctgccccc cttggttaca
     4921 ttccgacacc agaaacgctc ccgtttttta cgtgcatttg gcacggtatc gatggaggcc
     4981 ggggggggca atcctgaaca gaatgttact cctctgagta ttttctgtta agaaaatata
     5041 cacttagcac agttacaata aatcctaccc cctgtaagaa tgtgaattgt tcatccaaaa
     5101 acaaccatga caaaaataat gcaactagcg gaataaagta caacattatc cctgccaaat
     5161 gaactgggag ccaacgcaag gagttcaagt aaaaacaaaa agctaaaaaa tattgtaata
     5221 ttccagatga aaaaacaata atcacggatt ccaacggaag ataaattgag gatttagata
     5281 taaaaataaa gagcgatgac agtataaggg agaatgtcaa ctgtccgctc aataatgtga
     5341 taggctcaaa cgatttaata aggcgagacg atataacaac ataaaatgct gccgataaca
     5401 caccggcaac aacaagacaa tatccaacaa tattattcga tccattactg atgtctggca
     5461 aggctaccaa aatactgcca acaattgcac ctataaggaa caatataaaa tattgcgggg
     5521 aattaatttt aactgagcaa ataagaacag acaacaccaa tataaacact gattcaagtg
     5581 aaaaaataat tgatgcgctt cctgctgaaa taaaataaag tccgtagagt gtaaaagtat
     5641 acgctaagaa tggttcaaaa acgcctgtcg cataagcaag tatcgctttt gagtttaatg
     5701 ggagtttttt tctttttata aaaagaacta tccaagatag tattactgca ctacaaatct
     5761 gccagaaaaa cacccctccg gcatctagtt cttcaactcc ttttttgctc attacgatag
     5821 ctgcccccca tgagaacgct gctaaaaaag caaaaagcat tcctttaatc ata
//
