LOCUS       NZ_OY754459             6776 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F95 plasmid pF95-5, complete sequence.
ACCESSION   NZ_OY754459
VERSION     NZ_OY754459.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542931
            Assembly: GCF_963575635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:14:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,304
            CDSs (total)                      :: 5,179
            Genes (coding)                    :: 4,956
            CDSs (with protein)               :: 4,956
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 74 of 223
            Pseudo Genes (incomplete)         :: 143 of 223
            Pseudo Genes (internal stop)      :: 52 of 223
            Pseudo Genes (multiple problems)  :: 41 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754459.1.
            Complete genomic sequence of E. coli strain F95.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F95"
                     /db_xref="taxon:562"
                     /plasmid="pF95-5"
     gene            join(6520..6776,1..19)
                     /locus_tag="R8904_RS26410"
                     /old_locus_tag="FGAF95_50420"
     CDS             join(6520..6776,1..19)
                     /locus_tag="R8904_RS26410"
                     /old_locus_tag="FGAF95_50420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091752.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            complement(235..1128)
                     /locus_tag="R8904_RS26415"
                     /old_locus_tag="FGAF95_50370"
     CDS             complement(235..1128)
                     /locus_tag="R8904_RS26415"
                     /old_locus_tag="FGAF95_50370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277133.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(1657..1800)
                     /locus_tag="R8904_RS26420"
                     /old_locus_tag="FGAF95_50380"
     CDS             complement(1657..1800)
                     /locus_tag="R8904_RS26420"
                     /old_locus_tag="FGAF95_50380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001354641.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSSAELTGLE
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(1898..2227)
                     /locus_tag="R8904_RS26425"
                     /old_locus_tag="FGAF95_50390"
     CDS             complement(1898..2227)
                     /locus_tag="R8904_RS26425"
                     /old_locus_tag="FGAF95_50390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="WP_000182536.1"
                     /translation="MTNKLFEHTVLYDSGDAFFELKGNASMKLSPKAAIEVCNEAAKKG
                     LWILGIDGGHWLNPGFRIDSSASWTYDMPEEYKSKIPENNRLAIENIKDDIENGYTAFI
                     ITLKM"
                     /name="colicin transporter"
     gene            complement(2217..3890)
                     /locus_tag="R8904_RS26430"
                     /old_locus_tag="FGAF95_50400"
     CDS             complement(2217..3890)
                     /locus_tag="R8904_RS26430"
                     /old_locus_tag="FGAF95_50400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_045145925.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVSGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGRGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISASELSAAIAGIIAKLKKVNLKFTPFGVVLSSLIPSEIAKDDPNMMSKIVTSLPAD
                     DITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFT
                     ASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGH
                     NAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVAR
                     NQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQ
                     RAQTDVNNKQAAFDAAAKEKADADAALSTAMESRKKKEDNKRDAEGKLNDELEKNKGKI
                     PGLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGRNDPATVYG
                     SPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(4122..6341)
                     /gene="mobQ"
                     /locus_tag="R8904_RS26435"
                     /old_locus_tag="FGAF95_50410"
     CDS             complement(4122..6341)
                     /gene="mobQ"
                     /locus_tag="R8904_RS26435"
                     /old_locus_tag="FGAF95_50410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_098716998.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPVNAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKARPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPARRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITGLG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHERMARPERVRMYSGATVRHVLEQMRQGWPSYCFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCKGPEEVLVGFVRQEDGLRPV
                     LALPSPDYSHLIVSTITENGVCLAGYGEAINHDADTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     BGC             1..6776
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5984..6342
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.889"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6343..6513
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.93"
                     /coverage="97.0"
                     /evalue="5.74e-73"
     Typing          complement(236..1128)
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.275"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4126..6341)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.452"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaaccgcag aaccactaag ccccggcgcg taagtgccca tgcaccaccg gacgcctttg
       61 cgcttcctcg ctcattcgct cgctacgctc ggtcatgaga ctgcggcgag cgctaccacc
      121 tcaatcatgc ggaaagccac gccattacgg ggcaaaacgg aggaaatcgc aagaaagatc
      181 actgctcaaa aagacgccaa aagggcgctg ttatctgata aggcttatct ggtctcattt
      241 tgcgagatct ggatcacatt ttaattttcg gtagtacgtc gctcgactga tccctaatgc
      301 ctcccacggc ttcagcgaac gtgctgatgt aggaactgct gcgcgcttag atttagttcc
      361 gcccttgcga cctcgagcgg cctgtacagc ggaaaatccc tctggtgaga atttcctgtg
      421 tgtatatttc gcaatgctct tgccaatagc ccgacattca gccggagaaa gcggaacggg
      481 aagcgatgcg ttgtacattt cgacacgctg gatcacggca tcaagccatt gtgagaatac
      541 aggccagccc tgacgaatcg ctctgtaggc ccatttacgg accttttcga acagatggta
      601 gtttcgcccc agcccgtaat ttttatcgac gctacggcgc gctgaggcgc tcaaatcgag
      661 ataatcagcc agttcgtcga gagtgtaggg ttcctcgcgc cattccactt cctgccattc
      721 agggtggcac ggatttttgc agatcaggcc gctgtaattc acatccgcgc ccagtttttc
      781 acacaacgca cgctcaatag cggcagcgta tctgagcgcc gaagccgatg catccggcgc
      841 agttctcaca ggaagggcga gcgcgtagag cagatgcgcg tgcccgttac ggggattttt
      901 tacggtgatg ttcggggccg gacaattccg gtcgctccag tcgattgatg cccccgtccg
      961 gtccacgtca aagggaagcc agtacagtgc atgtggctgg ttctgctgga tgaaacgggc
     1021 aagaatggca atgtttttcg gcagaatgcg aacgccgtac gcgaaatcgt tcgtacagta
     1081 cggtttgtgc ggtaattttt ccctgaagcg ttgaagtacg gcgctcatgt gatacgattt
     1141 ctctcggtac acagtttaac taaccgcgct ttcccatcgg gaaacgcgga acccggcgcc
     1201 ctgtaatttc gtgatcaatc ttggcggtga gagcacgatt tacggggcgt ttgtttttca
     1261 gtcgcttaca ctacacccaa cggcaaatcc tgtcgaacct tcccgaaaac ccgatccatt
     1321 taccataact cccgttgtac gtaccctcta aaacgctctc agacgcgttc tgacgcaaaa
     1381 aacgcaaaaa gacaaggaaa caccaggctg aaccagaaaa atgcgtcaga atcgttttta
     1441 gggcgctgca taactatgca cgaaagcgat gcgcgatcgg tttcgaccgt tccgaatggc
     1501 gaacaggtca aaatgacaag ttttctccca ccggatggaa ggttctgcac agggatttta
     1561 gatcccatct cccgcatgga atttttgtga catttcaaaa aaaagcgcat cagaatcagc
     1621 ctgatacgct ttgttcttgt caaagaggat ttcgggttac tgcgtttcta atccggtcag
     1681 ttcagctgag gatgacgggg atacagtccc gccctgaaca tcccggatat agtttgcctg
     1741 acatgcagca agaataatga ctgcaagaag caataaaata atccctgtta tttttttcat
     1801 actgttcctt gtatatatgg actcaggcat tcatccataa aaacctgaaa tccaaaggtt
     1861 gaaatcatcc atgaaacata aaattctata acactattta catctttaac gtgataatga
     1921 aagcagtgta tccattctca atatcatctt taatattttc aatagccaat ctattatttt
     1981 cagggatttt tgatttgtat tcctccggca tatcatatgt ccatgatgct gaactatcta
     2041 tcctgaatcc aggattcagc caatgcccac catcaatgcc caaaatccat aagccttttt
     2101 tcgctgcttc attacaaact tctattgcgg cttttggtga taacttcata gaagcatttc
     2161 cttttaattc aaaaaaggca tcaccactat catataacac cgtatgttca aataatttat
     2221 tggtcatttt tatttcccca ttgaattctc gaatcgtcca cccaacccgg atccgtctta
     2281 tcactaatct gagtaacctc accagtacga tcattgacaa cgacatattt accaggagaa
     2341 ccatacactg ttgctggatc atttcgacct aaatagtctg gaggtgtttt cttcggacta
     2401 cgcttatcaa aagatgtacc agttgcaccg ttagatacgg tatttttgat atcatcctct
     2461 gtccatcctc tttctggcat ttgcccccta attttctgat ctatttttag acctggtatc
     2521 tttcctttat ttttttccaa ctcatcattt agtttgcctt cagcatctct tttattatcc
     2581 tctttcttct tcctgctttc catcgcagta cttaatgcag catcagcatc tgccttctct
     2641 tttgcagcag catcaaatgc agcctgctta ttatttacat ccgtctgcgc ccgctgagct
     2701 ttaagcccgg ccatttgcca cattctgtga ccgccagcca ttgggtcatg ggcaaatcga
     2761 ttaaattgtt ttatttcagc tattgcatca gcaagagttt tattcgctgc atcaagttcg
     2821 cttttacgcg aattataaac ctgaacagct ttagcctgtc gctcctgatt tctggcaaca
     2881 tcttcatttg cctgattcag ctctgcacgc gcgcgttcat aatttcgctc agccgcttca
     2941 accggatgcg tagcatccca ttcctgctga cggcgatttt cttcatcctg acgttgtttt
     3001 acctggtcag gactaagaac atcactcact gaaacatata cggcattatg accgctgtcc
     3061 ttcgggaatc gaataactgc atccctggta ttaccaccct gagtaaatcc tgccgggcga
     3121 acatccttat cagtattatt tgtaacacct gggcttaatg tctgtactgc tggcgtactg
     3181 ttattaactg aaatattcag aacaggtgca cctggaattg atgccgtaaa aacacctgga
     3241 cgttcggtag gttttgcatc aaccaccgga acactcatcg gaacacctga aacaaccgaa
     3301 atattctgtc gttcgtcttt tacatcatca acaacacgaa catttacgtt tactgttgcc
     3361 ttatcgagag gtaatgaact gacaggtgat tcagtaatat catctgcggg taatgacgtc
     3421 acaatctttg acatcatatt ggggtcatct ttcgctattt ccgacggaat taatgaagat
     3481 aagacaaccc caaaaggagt gaatttaaga tttacttttt ttaatttagc aataatacca
     3541 gcaatagctg ccgataattc gcttgcagaa atactgacag ccagaccgcc agctcctgga
     3601 gtggaaagtg ccggaaaacc aaatgccact ggcgcagcta ctgctgacaa attaccgcct
     3661 gttcccgagc caccaccgga attgccatta cccccgccat taccacgacc ggagccacct
     3721 ccccagtgaa tgccgctacc ggaaccacca ccccacgggt tattttccga actccatcct
     3781 gaaccatcag aagcaccacc acttacacca agcccggtcg ggccaccatt aatgttacca
     3841 cttgtgctat gcgcgcccgt gttatggccg cgtccatctc caccgctcat aaaagtccct
     3901 cttttaagcg ttaaaacaat taattacatg attaatactg tacataaaac cactggtttt
     3961 atgtacagat caaaaagtac ccgtcatcgt tttttctgtc aagttcaggt ggaaaatttt
     4021 ttagaagtca ggagggataa cacttagaaa acgcagagcg gaccacaaaa aattttgtgg
     4081 cccgtgatat gttggtaggg ttcggggggc agccccggat atcacatact cataccccgg
     4141 aagtgcccgg ggcggcgttg ttgttgcctg tgctgttcgt cccgcttcca ctcatgccag
     4201 gtctgattaa aaccgagttg acggaagaga taatccggca tctcttccgg tttgtggaca
     4261 gcagccaaaa gcctgtcatg atggcctttg agccggaact gcatcaggtg cggttccggt
     4321 gggtacggag tatccgcatc atggtttata gcttctccgt aacctgccag gcataccccg
     4381 ttctccgtga tggtgctgac tatcagatga ctgtaatccg gcgacggaag cgcaagaacg
     4441 ggacgcagcc cgtcctcctg gcgcacaaat ccgacaagca cttcctccgg ccctttgcac
     4501 aacagcagat tcagcgtcct gctgtttcgc tctgtcgaaa atacaacggg catgagttgc
     4561 tccggtgccg cataagcggt gacgtccacc cggtgcgcag acggcagcgg agaggctacg
     4621 ggcctgcggt cgtcgctgaa ataaaaatta tccggccagt gatgcggcag cgccggaaaa
     4681 cagtaagagg gccacccctg gcgcatctgt tccagtacat gccggaccgt cgcaccggaa
     4741 tacattctca cacgctcagg ccgcgccatc ctctcatgag attctggcat tccctcacgt
     4801 atatccgaga ccatcacagc cccgcctggc gacaggtaaa cattcaccgg gtcatgaaca
     4861 ctctgggggt aatgcgccag ctcatcaccc cataccgtga attctccggc tctgttactc
     4921 agcatcatgt agcgggttat cctgcccgtt cccggctctc tggcacgccc cagccccgta
     4981 ataaccccgg ctaatgccgt ttcacggctc aggcgatggt tatcatggtg tttcagccgt
     5041 gcgtcatatt cctgttcctt ccggcgcgct tccgcttcgg cttcctccct gcgccgctcc
     5101 gcttcccgtg ctgacagttt ttctttcagc tctgccattc ccggcgaaat ccacgtattc
     5161 cactgcgttt caaaggcctg acgacgggcg ggaatgtccc gttctttcac ggccctgtag
     5221 gtgcggagaa gagattcaat ctgtttctcg agtttccaga agcggagttg tcttttgcgg
     5281 tattccggtt cagcccatgt catcagggga agctctgaca ggatggacgc taccggacgg
     5341 gcttttctga gtccggtcat ctctttttcg agaatatccc gctctttcac cagcgcattc
     5401 ccctcacgtt tgatgtcgtt tgccttcccg ttcatcgcca cgaactccgg tgtcatctcc
     5461 catgcgttcc cgcattcctg cggcagtgtg gcaatgaatt cccgcagggt atccgctgag
     5521 gcattcgcgg cgggaatggt cagattaagg gcttccgctg cacgacgtgc ggcatcgctg
     5581 agttcccgtt tccgcacgtt ctcctgtacc tggatgactg ccagccttgt gcgggaaacc
     5641 tgtatttcca ggttaaggct gttaatcaga cggttctgct caccgcgttc tgtttccatg
     5701 ccccgttttt ccatcgcaca ggcagccttt ccgaggtgaa ggccgggcgc tttctccagt
     5761 ccctgacgct cgtatgaacg atggtctatc tcttcctggt agccggcgtt cgcaagcgct
     5821 ctgttcgcat ggtcagccca tgatgcgcgc cacgtctcgg cgagtgcccg gtcgttccag
     5881 tccctgacct ttcctgcgaa tcccgtttcc ccgacagctc tcgtggtcag catgatgtgt
     5941 gcatgggggt tggtccggtc catatgatgg aacgccacat cggcaatcat gccccgactg
     6001 acaaagtttt cccggacaaa agcgagaacg gcctcccgtg cggcctcgcg ggaaatctca
     6061 cggggaatgg cgagttcaat ctcccttgcc agctgggagt tccggcgctg ttcggctttc
     6121 tcgaccgcat tccagagaac ggaacggtca ccacaccagg acggtgcatt cacaggggtg
     6181 agaatgaccg catcatcaac gccgcgttta cgggtgtaat cgtgggttaa tccggtacga
     6241 tcatcgggta tgacagagcc ggaacggtag gcagcagaag caacggcact gtaaccgttt
     6301 tttcgcgaaa tgattttcat gctgagatga taaatcgcca tttcttccgt aatgcctttt
     6361 tgccgttttc tttcagcccc gcagggcgca cacaatcgaa acgaagtgaa gattgtataa
     6421 gtgcgcactt cgtgatgtgc cgtgcattat gccagttcag ttttgcaact accagtgacg
     6481 cgctaaaata cgatttttca ccgcgcacgg agtcacgaaa tgagcagaac actcgaacag
     6541 aagattgccg aagctgaggc cagattacag cgcctgaagg cgaagagccg gagcctggac
     6601 acggcgcaaa aagtcattgt gggggctgcc atgctggcga gggtacggag gccggaagaa
     6661 gcgcaactgc gtgcgttcct gctccagttt ctgcgaaagg aagtcacaag acaggcggat
     6721 gtgaaccgac tacaaccgct gattaacgag ctggagaagc tacccagacc accggc
//
