LOCUS       NZ_OY754454             3373 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F95 plasmid pF95-10, complete sequence.
ACCESSION   NZ_OY754454
VERSION     NZ_OY754454.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542931
            Assembly: GCF_963575635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:14:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,304
            CDSs (total)                      :: 5,179
            Genes (coding)                    :: 4,956
            CDSs (with protein)               :: 4,956
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 74 of 223
            Pseudo Genes (incomplete)         :: 143 of 223
            Pseudo Genes (internal stop)      :: 52 of 223
            Pseudo Genes (multiple problems)  :: 41 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754454.1.
            Complete genomic sequence of E. coli strain F95.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F95"
                     /db_xref="taxon:562"
                     /plasmid="pF95-10"
     gene            complement(563..796)
                     /locus_tag="R8904_RS24375"
     CDS             complement(563..796)
                     /locus_tag="R8904_RS24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(803..1363)
                     /locus_tag="R8904_RS24380"
     CDS             complement(803..1363)
                     /locus_tag="R8904_RS24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067486.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGMKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1392..2051)
                     /locus_tag="R8904_RS24385"
                     /pseudo=""
     CDS             complement(<1392..2051)
                     /locus_tag="R8904_RS24385"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2041..2364)
                     /locus_tag="R8904_RS24390"
                     /old_locus_tag="FGAF95_50610"
     CDS             complement(2041..2364)
                     /locus_tag="R8904_RS24390"
                     /old_locus_tag="FGAF95_50610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2428..2619
                     /locus_tag="R8904_RS24395"
                     /old_locus_tag="FGAF95_50620"
     CDS             2428..2619
                     /locus_tag="R8904_RS24395"
                     /old_locus_tag="FGAF95_50620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2701..2781)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.805"
                     /coverage="93.0"
                     /evalue="8.68e-18"
     Typing          complement(481..2051)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.439"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcggtcgccg tcgtcacagc tcccccaacc gcaaacgatt ctcgcaaata cgcaagtatc
       61 tgcaaaaaca ggtttttttc ggggttcctg ggagactgtg ctgttgaaaa attcttgaag
      121 aatacagaag gctcatgcaa actgaatgaa ctggtttctg ttgtggatct caacagtttc
      181 cacaggaaca ttgaggaggt cagagacctc aatgaacccg aaacagctcg ccttcctggc
      241 gagctgtttt cgttctacgc ccgccgacga aagatcgcaa gtgcaataac gagatttttc
      301 acgtctttac gctcaaaaag taaaaaaacc atgttttccc ctggctgaat ccccaaaaac
      361 gcgtgaaatg cgttttcagg atgttgcata gctatgcaca aatacggcac acgatttgtt
      421 tcgctcgccc cagccgtcga acgggtcaaa atggcaagat ttcgtcactt ccccggtcac
      481 atctccattg atggcccact ccgaacccgt gccgcgcgtt cctgtacctc atgtctgtac
      541 cgggcacgct cctgttcatc ccttagctgt tgttcgcgat aaataaccga ctcaagcgat
      601 ccacttgccc ggctaatctc tgcacctgct gactcaatat cacgacgcgc tcgttcagta
      661 tcgcgttgtt ccgttgcgta acctcgtgca tcttctgcaa ttccgcgaag gcgctctccc
      721 actcgttcag ccgccgcata tagtcctgtt gcaaatgctc taaggcgttc agcaaatgcc
      781 cttccagttc cgtcatactc gtttactccc tgaccagcct cacggcgttc ttgtcgccca
      841 ccgtccaggt gttttcccct ttcatcccgt ccggcaacac cagaaatttc ccgttctcgc
      901 cattgacgaa cgtcaccccc caggtttttg cctcgagttt cgccagcgtg ttttcctgct
      961 ctctgatagt caggatattt ttcgcaatct tgctttcctg ccactccatc agcccccagc
     1021 accccaggaa aatcccgccc gtgacgctga gcgcggtcag cgccggaaac agtatccgcc
     1081 ctttgatcac ccgccagagc agttttttct gctgccggga cagttcggcc tctgtggtgc
     1141 tgaactgcat gctcacggtg gtgctcagcg tatccagttg ctttttcacg gatgttgtat
     1201 gttcgctgat agcgtctctg attttctgcc tgtttaagct caatgccttg tctacagact
     1261 cttcgagctt cctgaactcg ctgttcagca tgttctcggt agaagcggct cgctctttca
     1321 gtttcttctc gaagtctgtt cccatttgta aaagattgct catacagcgc cccctttagc
     1381 ctgagattcc gccccccctc cgggtcggca atactgatgc tgcttttggt tgtcctcacc
     1441 acctcaaaac ctgccgctgt aagcgcctct gtgacatcct gacgcgtttt tattgctccg
     1501 gcatggtaaa gagcctcaag accccgtgta atcgcttctg cggcctcctg tttcgctttt
     1561 ggcagattat ttggcgtgac aagcgtccgt ctgttctccg gtgcgttcgg gtcacgcagc
     1621 ccgtaatgat gattaaccag cgtctgccag gcatttatcc tcggcctgtc cgcacggtcg
     1681 tagtacggct ggaggcgctt accgctctgt agctccatgt tcgggatgac aaaattcagt
     1741 tcgagccgcc ccttatcctg gtgttccacc cagagaatgc tgtactggtc tttttcgaga
     1801 ccgggcataa gaacacgctc aaagctcgtc atcactcttt cgcgttcacc cggtggtagc
     1861 tccttctccg cgaacgacag aacgcctgac gtgtatttct tcgaaaatgg cgtggaatca
     1921 atgagttccc gcacttcttc cgggttaccc cggagcaccg ttgcgccttc gcggttacgc
     1981 tcccgcccca gcaggtaatc gaccgggcca cttccaccgc ctttccccct ggcatgaaat
     2041 ttaactatca tcccgtaccc cctgttccct gacagcctgc cgcaactggc gcaattcacc
     2101 ctcgatggcc atcagtgcgg ccaccacatg aacccggtca atcgaagacc actggccgct
     2161 gttcaccttc cgggctgtct ggttcaggtt gttcccgatg gcagccagat gccgcagcaa
     2221 tggcggtgcc agtgtcggca gttttcctgt gcgtgcaacc ggctccccca gacagacccg
     2281 ccgcatccat tccgccagtc gtttaccctc gcagcgttcc agtaaccggg cgtgctcctc
     2341 atcggtaacc cgtatcgtga gcatcttctc gcgtttcatc ggtatcattc cccctaacaa
     2401 atcccccata caaggagcct taagaccatg accaaacagg aaaaaacagc ccttaacatg
     2461 gctcgcttta tcagaagcca gacgctaacc ctgctggaaa aactcaacga tctggacgcg
     2521 gacgaacagg cagacatctg cgaatcactt cacgaccacg ccgacgaact ctaccgcagt
     2581 tgcctcgcac gcttcggcga tgacggtgaa agcaactgaa acatgcagct cccggagacg
     2641 ttcacagctt gcctgtgagc ggatgccggg agcagacaag cccgccaggg cgcgtcagtg
     2701 cgggtgtcgg ggcgcagccc tgacccagtt cacagagcga tagcgatgtg tatactggct
     2761 tacctcattt tgcatcggtg cagagtggat gggaagtgca ccatgtcagg agtaaaaagc
     2821 cgcacagatg cgtaaaaaca gcgcgcccga taaacaacac acacgcggac actggcacag
     2881 gcgcgtaagt gcacaatgct tgcctctgtg actcttccgc ttcctcgccc actcgctcac
     2941 tacgttcggt cgctggggct gcggcgagcg gtgggatcac tctcgttgtg atgcagatcc
     3001 gttttttttt ttgcactgga gttatcagga gtagcggtgc acatgggatc gtgcgatatg
     3061 ctggatggtg gaaggtcgga gggaacggca acaactcacc cctcccagtg aagggacggg
     3121 tagtttgccg ctctcgatat gccggacgga gccaaggcca tcgatcacgg ggcgcgtcag
     3181 tggaagaacc cgcgcctgcg tgacgtatgc gtcttcgccg caccatctca cccgtcacgc
     3241 caccacatac cgggaactgc cgccaaggtc taactcaacc aatctagccg cgctacatac
     3301 ggtttttgtg gcaactcatc aggtgtccgc tgtcgatgaa actctctgcg ccggtagcgg
     3361 aactctcacg cgt
//
