LOCUS       NZ_OY754447             5873 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F829 plasmid pF829-4, complete sequence.
ACCESSION   NZ_OY754447
VERSION     NZ_OY754447.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542912
            Assembly: GCF_963575575.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 09:58:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,900
            CDSs (total)                      :: 4,780
            Genes (coding)                    :: 4,517
            CDSs (with protein)               :: 4,517
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 70 of 263
            Pseudo Genes (incomplete)         :: 184 of 263
            Pseudo Genes (internal stop)      :: 50 of 263
            Pseudo Genes (multiple problems)  :: 42 of 263
            Pseudo Genes (short protein)      :: 4 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754447.1.
            Complete genomic sequence of E. coli strain F829.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F829"
                     /db_xref="taxon:562"
                     /plasmid="pF829-4"
     gene            complement(join(5723..5873,1..380))
                     /locus_tag="R8822_RS24465"
     CDS             complement(join(5723..5873,1..380))
                     /locus_tag="R8822_RS24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<418..1116)
                     /locus_tag="R8822_RS24470"
                     /pseudo=""
     CDS             complement(<418..1116)
                     /locus_tag="R8822_RS24470"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1058..1381)
                     /locus_tag="R8822_RS24475"
                     /old_locus_tag="FGAF829_47050"
     CDS             complement(1058..1381)
                     /locus_tag="R8822_RS24475"
                     /old_locus_tag="FGAF829_47050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024188420.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAAWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALIAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1447..1638
                     /locus_tag="R8822_RS24480"
                     /old_locus_tag="FGAF829_47060"
     CDS             1447..1638
                     /locus_tag="R8822_RS24480"
                     /old_locus_tag="FGAF829_47060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021539356.1"
                     /translation="MTKQEKTALNMVRFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1793..2137)
                     /locus_tag="R8822_RS24485"
     CDS             complement(1793..2137)
                     /locus_tag="R8822_RS24485"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_317731062.1"
                     /translation="MSRKAPTDAPKKCLFCEVRQGGGAYGKSASGAAFSGVSFFAHMFF
                     PGLSPGSVDNRVSVFEKGESARRSRTTERSESVSEEAEERLNWHFLLTHLCGISHPTWR
                     TFHTIRTDAE"
                     /name="hypothetical protein"
     gene            complement(2843..3709)
                     /locus_tag="R8822_RS24490"
                     /old_locus_tag="FGAF829_47070"
     CDS             complement(2843..3709)
                     /locus_tag="R8822_RS24490"
                     /old_locus_tag="FGAF829_47070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(3711..4763)
                     /locus_tag="R8822_RS24495"
                     /old_locus_tag="FGAF829_47080"
     CDS             complement(3711..4763)
                     /locus_tag="R8822_RS24495"
                     /old_locus_tag="FGAF829_47080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021517772.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGNGGYSHPEGISTTWHSESKLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIDNSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGVPVDDASMFFNSFQPRKFE
                     NNPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(5483..5716)
                     /locus_tag="R8822_RS24500"
                     /old_locus_tag="FGAF829_47100"
     CDS             complement(5483..5716)
                     /locus_tag="R8822_RS24500"
                     /old_locus_tag="FGAF829_47100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021520366.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1733..1814)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          complement(2271..2458)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(17..1068)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(5380..5852)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.468"
                     /coverage="98.0"
                     /evalue="7.410000000000001e-80"
ORIGIN
        1 ctctctttct gctcgcggag aatattcata ttcgcgttta tcttcatgcc ctggtaccac
       61 agcagactcc cggtcgtggc aaacatcagt actgagacga taatcagcca cagccagcgt
      121 ttcacgctca ttttcagtac ggttttccag tgcgcgtcca tcgtatcgac ggtcgttttc
      181 tgatacctct ccagcagttc tttcagggct gttgtatggt cgttgatagc gttcctgatt
      241 aaagattcgt ttgaggtcag ttccctgttt acagattctt cgagcttcct gaactcgtcg
      301 ttcagcattt tctctgtaga ttcggctctc tctttcagct ttttctcgaa ctccgcgccc
      361 gtctgtaaaa gattgctcat aaaacgctcc tttcagtctg atattcctcc cgccgttcgg
      421 gtctgcaatg ctgatactgc ttcgcgtcac cctgaccact tcaagccccg cctctgtgag
      481 cgcctgaatc acatcctgac ggccttttat ctctccggca tggtaaagcg cgtctatccc
      541 gcgtgtgacg ccctcagcaa gcgcctgttt cgtttccggc aggttatcag gaagggtcag
      601 cgtccggcgg ttctccgggg cgttggggtc atgcagaccg taatggtgat tcaccagcgt
      661 ctgccaggca tcaattctcg gcctgtctgc ccggtcgtag tacggctgga ggcgttttcc
      721 ggtctgtagc tccatgttcg gaatgacaaa attcagctca agccgtccct tgtcctggtg
      781 ctccacccac aggatgctgt actgattttt ctcgagaccg ggcatcagta cacgctcaaa
      841 gctcgccatc actttttcac gccctcccgg cggcagctcc ttctccgcga acgacagaac
      901 acctgacgtg tatttcttcg caaatggcgt ggcatcgatg agttcccgga cctcttccgg
      961 attcccctga agcaccgttg ccccttcccg gttacgctcc cttcccagca ggtaatcaac
     1021 cggaccactg ccaccacctt ttcccctggc atgaaactta actatcatcc cgcgccccct
     1081 gttccctgac cgccagacgc agccggcgca gctcatcccc gatggctatc agtgcggcca
     1141 ccacctgaac ccggtcaccg gaagaccact gcccgctgtt caccttcctc gctgtctggt
     1201 taaggttatt cccgatagcg gccagctgac gcagtaacgg cggtgccagt gtcggcagct
     1261 tcccggaacg ggcaacaggc tcacccaggc agacccgccg catccatgcc gccagttgtt
     1321 taccctcaca gcgttccagt aaacgggcat gctcatcatc agtcacccgt atcgtgagca
     1381 tcctctcgcg tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg
     1441 catccagtga ctaaacagga aaaaaccgcc cttaacatgg tccgctttat cagaagccag
     1501 acattaacgc tgctggagaa gctcaacgaa ctggacgcgg atgaacaggc agacatctgt
     1561 gaatcgcttc acgaccacgc cgatgagctt taccgcagtt gcctcgcacg cttcggggat
     1621 gacggtgaaa accgctgaca catgcagctc ccggagacgg tcacagcttg tctgtgagcg
     1681 gatgccggga gcagacaagc ccgccagggc gcgtcaggcc gttgaccttt ggggtgtcgg
     1741 ggcgaagccc tgacccagtc acgtagcgat agcggagtgt atactggctt aatcattcgg
     1801 catcagtgcg gattgtatga aaagtgcgcc atgtcgggtg tgaaatgccg cacagatgcg
     1861 taaggagaaa atgccagttc aggcgctctt ccgcttcctc gctcactgac tcgctacgct
     1921 cggtcgttcg gctgcggcga gcggactctc ctttctcaaa aacgctaacc ctgttatcca
     1981 cagaacccgg ggataaacca ggaaagaaca tgtgcgcaaa aaacgaaacc ccggaaaagg
     2041 ccgcgccgga ggcgcttttt ccataggctc cgcccccctg acggacttca caaaacagac
     2101 actttttcgg tgcatctgtg ggagccttgc ggctcaacca tgaaatccgc cagcggtggc
     2161 gaaacccgac gtggcttaaa gatcatcacc gtttccggtg aatcgctccc tcctgcgccc
     2221 ccacgttccc gccatatcga ttaccggata ccttttccgc ctttctccct tacgggacac
     2281 gtggcgcttt ctcatagctc acgtcttctg gtatctcggg gcgacttagg cagttcgctc
     2341 cgggctgggc tgtatgcagg aaccccccgt tcagcccgac tgctgcgcct tctccggtaa
     2401 ctgttctctt gggtccaacc cggaaaagac accacaaagc gccactggca gcagccattg
     2461 gtaaaagtca tgtgcgagat atttagagag tacttgaagt ggctgccgga gtgcggctac
     2521 actagaagaa caattttggt gactcgtctc gcataagaca gttacctggt taagcagtct
     2581 gcaagctgac ttaaccttcg atcaaaccgc cctgcaaggc ggttttttcg tttacaggca
     2641 gcagattacg cgcagaaaaa aaggatctca agaggatcct ttgatctttt tgttacaaac
     2701 gtctctattt gactcccctg agagcgtttt agcgcgcctc tgcccccctt ggttacattc
     2761 cgacaccaga aacgctcccg ttttttacgt gcatttggca cggtatcgat ggaggccggg
     2821 gggggcaatc ctgaacagaa tgttactcct ctgagtattt tctgttaaga aaatatacac
     2881 ttagcacagt tacaataaat cctaccccct gtaagaatgt gaattgttca tccaaaaaca
     2941 accatgacaa aaataatgca actagcggaa taaagtacaa cattatccct gccaaatgaa
     3001 ctgggagcca acgcaaggag ttcaagtaaa aacaaaaagc taaaaaatat tgtaatattc
     3061 cagatgaaaa aacaataatc acggattcca acggaagata aattgaggat ttagatataa
     3121 aaataaagag cgatgacagt ataagggaga atgtcaactg tccgctcaat aatgtgatag
     3181 gctcaaacga tttaataagg cgagacgata taacaacata aaatgctgcc gataacacac
     3241 cggcaacaac aagacaatat ccaacaatat tattcgatcc attactgatg tctggcaagg
     3301 ctaccaaaat actgccaaca attgcaccta taaggaacaa tataaaatat tgcggggaat
     3361 taattttaac tgagcaaata agaacagaca acaccaatat aaacactgat tcaagtgaaa
     3421 aaataattga tgcgcttcct gctgaaataa aataaagtcc gtagagtgta aaagtatacg
     3481 ctaagaatgg ttcaaaaacg cctgtcgcat aagcaagtat cgcttttgag tttaatggga
     3541 gtttttttct ttttataaaa agaactatcc aagatagtat tactgcacta caaatctgcc
     3601 agaaaaacac ccctccggca tctagttctt caactccttt tttgctcatt acgatagctg
     3661 ccccccatga gaacgctgct aaaaaagcaa aaagcattcc tttaatcata ttaatcatcc
     3721 tctttctcat aaagggggtt gttttcaaat ttccttggtt gaaaggagtt aaaaaacata
     3781 gatgcatcat ctacaggaac accaataaac tctccataat aaggatcatc tataggaaac
     3841 tcttctaatg aaacataaaa caatacattc cagttgaaga tcattggatc actattatct
     3901 atttcatgga gcaatctttc atccttagtt aattctcctg gaattaatac atcagtatga
     3961 taaaaaccgc tgtaataaga actattaccg gtgtgcactg ggcatccaaa acgatccaat
     4021 ttagattctg aatgccatgt tgtagaaatc ccttccggat gtgaataccc accgttacca
     4081 acagactgaa tagtcatgtt acgaatatcc gctaacgctt gttcttgatc tttatagaaa
     4141 cctttttcta tcgccagcct cataaaatgc cccggggcat ttacagaaat aagtattccc
     4201 cccggtattc ttctatcatg atttcttgtt ctatgagcag aagagtaaaa aacattaaca
     4261 ccagccttat atatttttag cccatcttta tcctgaaatg gtacgctttc agtataaata
     4321 acatcctctt ttattggata gcactctggc atatgcagcg aacaaatata tttttgtatg
     4381 tccactaact cttctgatgg acgagcaaat gcaagttgtg gatgaataaa ataaatgaga
     4441 tgcgcactga cctctcctct tcttgcgcgt tctttaaaac gaactctttc attacgcaaa
     4501 aaatcaaaca aatgaccatc atcattatat agttgggggc tatttacaat agataaatgg
     4561 aaatcaagcc ctttaatttt cttgcttgcc aatttaccaa atacacaggg ctgattgcct
     4621 gacacccatt ttcttagtaa atcttcaatt ttatctttag aagtttcgtt aaagataaat
     4681 gaatgaatat tatcaatatc ttttccaaat gaaaaactgg atgataattg tggtagttta
     4741 tttatacgtt gttgaagatt catgaaagct cccacaagac acctcatata ttttttatat
     4801 actgaaacta cattatattt attaccgacg taaaacgaaa taatttatta tcgttaccga
     4861 tattttaaaa aatcattatt tgcacagtgt tcgagttgtt agtatcactc cttcctgaaa
     4921 atccgctcca tttaccataa cgtcgcattg tacgcaccgc caaaacgcgt tcggtgcgat
     4981 cacggcagca gacaggtaaa aatggcaaca aaccacccga aaaactgccg cgatcgcacc
     5041 cgataaattt taaccacatg catagctatg cagccatgtg aatcacgcag gaattgccag
     5101 ccagagactg ctgaaacgga tttttttcat actttcggaa aggcagaaga cggaaccgga
     5161 gcggaaaaac agaaattcat gggaatgagc gcgatctggc aataacggca aaacacagca
     5221 acaaagacac accagaatcg cgcccgcatg cgttttaacg cgttcctgtg ttttcagcaa
     5281 gctctgactg aagcgcgttc ccggtgcccc cgaaccaacc attatcccaa aaaatgcacc
     5341 ggaaacgcgc tgccaacatt ttagcctgtc tctggttacc atcccagcga agggccatcc
     5401 agtgtccgtt cctgaatttc cggctggcgc tcccgctcca gggataacgc atattcgcgc
     5461 tcctgtatca gccgctcatc ccttatctcc agttctcgct gtataactgg ctcaagcgtt
     5521 ctgtccgctc gctcaagtgc tgcacctgct gactcaactg catgactcgc ccgttcagca
     5581 tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa ttccacgaag gcgttctccc
     5641 attcgttcag ccgctgcata tagtcctgtt gcagttgctc taatgcgttc agcaaatgtc
     5701 tttccagctc cgtcatgctc tgtcactcct gcaccagtct cactgcgttc agtttgccgt
     5761 tgtccttcgt ccagttcgtt tcggctttca tccccttcgg caacaccaga aaacgtccgt
     5821 tgctgtcctc gtgataacgc acgccccatg tcttcgcgtt cagcttctcc aga
//
