LOCUS       NZ_OY754430             4091 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F200 plasmid pF200-3, complete sequence.
ACCESSION   NZ_OY754430
VERSION     NZ_OY754430.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542875
            Assembly: GCF_963575505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:16:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,005
            CDSs (total)                      :: 4,884
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 88 of 194
            Pseudo Genes (incomplete)         :: 126 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 57 of 194
            Pseudo Genes (short protein)      :: 2 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754430.1.
            Complete genomic sequence of E. coli strain F200.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F200"
                     /db_xref="taxon:562"
                     /plasmid="pF200-3"
     gene            complement(join(2109..4091,1..165))
                     /gene="mobQ"
                     /locus_tag="R8804_RS25005"
                     /old_locus_tag="FGAF200_47660"
     CDS             complement(join(2109..4091,1..165))
                     /gene="mobQ"
                     /locus_tag="R8804_RS25005"
                     /old_locus_tag="FGAF200_47660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_061089184.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRSRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     QRDEYARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            336..626
                     /locus_tag="R8804_RS25010"
                     /old_locus_tag="FGAF200_47640"
     CDS             336..626
                     /locus_tag="R8804_RS25010"
                     /old_locus_tag="FGAF200_47640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021533561.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVREP
                     GETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     gene            1159..1860
                     /locus_tag="R8804_RS25015"
                     /old_locus_tag="FGAF200_47650"
     CDS             1159..1860
                     /locus_tag="R8804_RS25015"
                     /old_locus_tag="FGAF200_47650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          2..165
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.07e-80"
     Typing          182..332
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.81"
                     /coverage="85.0"
                     /evalue="3.53e-55"
     Typing          3902..4091
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.29e-95"
     Typing          1160..1859
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.301"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2113..4091)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.242"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgtcagaatc actgcgctgg ctacaccgtt tttgcgggtg tagtcgtgaa tcactccggt
       61 gcgctcatcc agtatttttt ctccggcacg ataggcagca gaagcaacgg cgctgtagcc
      121 cttgcttctg gaaagaatct tcatgctgag atgataaatt gccatcgtgt cgttgttgcc
      181 gttttctttt agccccgcag ggcgcacaca aactcgacga aggagagttt gtataagtgc
      241 gcacttcgtg atttgctgtg cattatgcca gttcagtttt taaactacca gtgacgcgct
      301 aaaatgcgat ttttcaccgc gcacggagtt acggaatgag tagaacactc gaacagaaga
      361 ttgccgatgc tgaggccaga ttgcagcgtc tgaaggcgaa gagcaggagc ctggacacag
      421 cgcagaaggt cattgtggga gcggcattgc tggcaaaggt cagggagcct ggggaaacac
      481 aactacgagc ctggttgctt catctcctga agacagaggt aacacggcag gctgatgtga
      541 gcaggataca gccgttgatt gacgaactga acgcgttacc aaaaccagtc cccaaagaag
      601 tgtcagaaaa cgaccaacag gcgtgaatgc accgtccgct cgccgcagcc ccagcgaccg
      661 agcgttagcg agcgagtggg cgaggaagcg gaagtgaatc cgtcgccaga ttgagcacat
      721 acgcacggtt tttcgacagg tgatatgcgc tgaacccgca tgaatactgg atttccacag
      781 ggttgagcgg ggttttgcta aagcttgcga acctgctttt ttgtaacctg ttgttttcca
      841 tgattttttg ttttgaaccc cccaagtttt ccacaggctc gccgttaagt ttttagatct
      901 tgtttttaag atcttgtttt taatacttgt tttagggtcg aattttctta tgaaattcaa
      961 gagtttagaa gactataggt aatataattt aaggtaaagg caatatgatt taaggtggat
     1021 aggcaatatg atttaaggta aaggcaatat gatttaaggt aatattcaca ggaaaaggga
     1081 tattaaaatt aaatcatatt gcctttacct aaaggaatgt ttatatttta ctcattacct
     1141 taggttgagg gatttacaat gtcggatctg attgcctaca aatccaatgc actggttgag
     1201 gccagctata aattgactct tcaggaacag cgattcttgt tactttgcat tggtcgattg
     1261 aagtcgggtt ctgatgcaga atccactgaa ttgcaaaaga ccatgactgt taccgccgaa
     1321 gagtatttcg actcattccc tgatatgggg cggaaaaatg ccgaggttca gctacaggaa
     1381 gcaattgatc gtttgtggga caggtcgatc atccttaagg acgatgagaa gcgcgaggag
     1441 ttccgctgga tccagtatcg ggcacagtac gctaaaggtg agggcaaagc ccaaatcact
     1501 ttttcagatg cggtcatgcc ttatctgaca cagttgaagg ggcaatttac tagagttgta
     1561 attaagaaca tctccaattt atcaagaagt tacagcataa gaatatacga acttctccag
     1621 caattccgca gcacagggga acgtattatc gccctcgatg attttcgctc aatgcttgat
     1681 atagagcata aatatcaaac atataagtct ctgaaccagc aattattaag accttgcgtt
     1741 gacgaactga atcaaaaaag cgacttggct gtaacgcttg agacgatcaa gaaggggcgc
     1801 acggttgtag ccctgcattt tcgcttcaag gaagacaaac agatcaagat gacgatctag
     1861 aatcgatttt aaggcctcta aaacgctctc tgacgcgttt tttttgtgtt tggcatgcga
     1921 aggtactggt gaagtaaaaa attgcgtcat aaacgctctg gcacagtgaa aaaagaaccc
     1981 ccaatttttc gggggcggga aaactataaa gttgatttgt gttatgtgtg tgcttcttgc
     2041 attcatccct gtcgatgaac atcgacgaac ataacacgat gttaaacaaa acaaaatatt
     2101 ggtcacattc actgtgacca accgcgatca tgtctcctct cggtggcgtg tgttcgggca
     2161 tattcatcac gttgccattc atcccaggtg cgctcaaagc ccagcgagcg gaacagatga
     2221 tccggctgtt tttctggcgt gttgacgata gcaaaaagcg gttcgctcca ggttttcaga
     2281 tgaaactgca taagttccgg cgcaggacgt gccggactgt cagcatcgtg gtttaccgct
     2341 tcaccatagc ctgcaaagcg taaacctttg tcggtcagtg cattgattac cagctggctg
     2401 ttgtcggctg tggggacggc aaacgcggga tacagcgtac cattaagccg gacaacaccg
     2461 cccatagagt catccacacc catgaccagc aaaagctgaa gctggcgggt ttcacggtcg
     2521 acagaactca ttaccggcat cagagtcggt ggcagacgtc cctgcgtaaa atccaccttc
     2581 cttgccgcca gtgatggcat ggctggctgg cggtcttctc caagacagaa agactccggc
     2641 catgttttcg gctggcggag attacgggta tgttgcggcc atgtctggca aacctgcgtc
     2701 agcagttgct caaaggtcgc agaggaataa acacgttgca gtggtggacg ttgaccgatt
     2761 tcgcttcggt cacagatata accactcctg tcacgcatta aatcaacact ctcaaaaatt
     2821 ctggagcggt atttctccag ctcgttcccc cagagcgtat attctccgtt ccggttgtga
     2881 agcagaaggt aacaggtcat ttccccgtta cctggcatgg gggcctcgcc ataccccgta
     2941 acgactgctt tcagtacccc tgaatccata acggcattgt catttctcct gagctgctcc
     3001 gcccgacgac gttctgtttc ctcctgctcc ttccggcgca attcctcttc acgtttacgc
     3061 aactgttctc ggagatctgc aaggccagat gcaccccact gctgccactg tgtttcaaat
     3121 gctttttgac tcgcgggaat gtcctgtttc tcaaccttcc agtgatgctg ccgcaatttt
     3181 tccatgcgct ggctcaacga ctggattttt tcctgttcgc ggccgtattc cggtgcaacc
     3241 cattgcatca ggggaatacg ggaaaaaccg gacgaaaacg gcgatgattt tttcaggctg
     3301 gcagcatggc tcatcgcttc ctttcgttcg gtgttcagat cttcccagcg tctgcgcgtc
     3361 gcctgcatat ctgccatcat cgacatggct ttactggtga gtttccatgc tgcgttactg
     3421 tccgtcggca acgcttcgat gatgtcatgc agtatgtacg aagttgcgtt gtcaggaagc
     3481 tcaatattca gaaggtcagc catacggcgt gcgggctctg tcagctcact gttcctgagt
     3541 tcgtcgcggg aaacctgtat ttccaggtta agcctgttaa tcaggcgatt ctgttcgccg
     3601 cgttctgttt cgatgcccca cttttccatc gcacaggccg actttccgag gtgaatgcca
     3661 ggcgttttct caaccccctg tcgctcgtat gaacgatggt ctatctcttc ctgataaccg
     3721 gcgttcgcca gcgccctgtt cgcatggtct gcccatgatc cccgccatgt ttcggcgtga
     3781 gtccagtcat tccactcacg gactttctcc ccgaagccgt caggcgtgat tgccctcatg
     3841 gatagcatga tgtgcgcgtg tgggtttgac ccgcccagat tgtgaaaggc cacgtcggcg
     3901 atcatgccgc gactgacaaa gttttccctt acgaagttga gaactgtttc ccgtgcggca
     3961 tcctgcgcca gttcacgggg gatggcgagt tcgaactccc gcgcaagttg tgagtttttc
     4021 cgccgctcgg ctttttcaac agcattccag agttcggcgc ggttcgcaca ccatgctggg
     4081 gcgtttgctg g
//
