LOCUS       NZ_OY754423             5873 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F344 plasmid pF344-2, complete sequence.
ACCESSION   NZ_OY754423
VERSION     NZ_OY754423.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542946
            Assembly: GCF_963575485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:00:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,159
            CDSs (total)                      :: 5,036
            Genes (coding)                    :: 4,875
            CDSs (with protein)               :: 4,875
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 63 of 161
            Pseudo Genes (incomplete)         :: 105 of 161
            Pseudo Genes (internal stop)      :: 31 of 161
            Pseudo Genes (multiple problems)  :: 37 of 161
            Pseudo Genes (short protein)      :: 5 of 161
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754423.1.
            Complete genomic sequence of E. coli strain F344.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F344"
                     /db_xref="taxon:562"
                     /plasmid="pF344-2"
     gene            complement(join(5495..5873,1..674))
                     /locus_tag="R8830_RS25765"
     CDS             complement(join(5495..5873,1..674))
                     /locus_tag="R8830_RS25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021517772.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGNGGYSHPEGISTTWHSESKLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIDNSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGVPVDDASMFFNSFQPRKFE
                     NNPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(1394..1627)
                     /locus_tag="R8830_RS25770"
                     /old_locus_tag="FGAF344_49170"
     CDS             complement(1394..1627)
                     /locus_tag="R8830_RS25770"
                     /old_locus_tag="FGAF344_49170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021520366.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1634..2164)
                     /locus_tag="R8830_RS25775"
                     /old_locus_tag="FGAF344_49180"
     CDS             complement(1634..2164)
                     /locus_tag="R8830_RS25775"
                     /old_locus_tag="FGAF344_49180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2202..2900)
                     /locus_tag="R8830_RS25780"
                     /pseudo=""
     CDS             complement(<2202..2900)
                     /locus_tag="R8830_RS25780"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2842..3165)
                     /locus_tag="R8830_RS25785"
                     /old_locus_tag="FGAF344_49190"
     CDS             complement(2842..3165)
                     /locus_tag="R8830_RS25785"
                     /old_locus_tag="FGAF344_49190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024188420.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAAWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALIAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3231..3422
                     /locus_tag="R8830_RS25790"
                     /old_locus_tag="FGAF344_49200"
     CDS             3231..3422
                     /locus_tag="R8830_RS25790"
                     /old_locus_tag="FGAF344_49200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021539356.1"
                     /translation="MTKQEKTALNMVRFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4627..5493)
                     /locus_tag="R8830_RS25795"
                     /old_locus_tag="FGAF344_49210"
     CDS             complement(4627..5493)
                     /locus_tag="R8830_RS25795"
                     /old_locus_tag="FGAF344_49210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     Typing          complement(3517..3598)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          complement(4055..4242)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(1291..2852)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.232"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atagtcatgt tacgaatatc cgctaacgct tgttcttgat ctttatagaa acctttttct
       61 atcgccagcc tcataaaatg ccccggggca tttacagaaa taagtattcc ccccggtatt
      121 cttctatcat gatttcttgt tctatgagca gaagagtaaa aaacattaac accagcctta
      181 tatattttta gcccatcttt atcctgaaat ggtacgcttt cagtataaat aacatcctct
      241 tttattggat agcactctgg catatgcagc gaacaaatat atttttgtat gtccactaac
      301 tcttctgatg gacgagcaaa tgcaagttgt ggatgaataa aataaatgag atgcgcactg
      361 acctctcctc ttcttgcgcg ttctttaaaa cgaactcttt cattacgcaa aaaatcaaac
      421 aaatgaccat catcattata tagttggggg ctatttacaa tagataaatg gaaatcaagc
      481 cctttaattt tcttgcttgc caatttacca aatacacagg gctgattgcc tgacacccat
      541 tttcttagta aatcttcaat tttatcttta gaagtttcgt taaagataaa tgaatgaata
      601 ttatcaatat cttttccaaa tgaaaaactg gatgataatt gtggtagttt atttatacgt
      661 tgttgaagat tcatgaaagc tcccacaaga cacctcatat attttttata tactgaaact
      721 acattatatt tattaccgac gtaaaacgaa ataatttatt atcgttaccg atattttaaa
      781 aaatcattat ttgcacagtg ttcgagttgt tagtatcact ccttcctgaa aatccgctcc
      841 atttaccata acgtcgcatt gtacgcaccg ccaaaacgcg ttcggtgcga tcacggcagc
      901 agacaggtaa aaatggcaac aaaccacccg aaaaactgcc gcgatcgcac ccgataaatt
      961 ttaaccacat gcatagctat gcagccatgt gaatcacgca ggaattgcca gccagagact
     1021 gctgaaacgg atttttttca tactttcgga aaggcagaag acggaaccgg agcggaaaaa
     1081 cagaaattca tgggaatgag cgcgatctgg caataacggc aaaacacagc aacaaagaca
     1141 caccagaatc gcgcccgcat gcgttttaac gcgttcctgt gttttcagca agctctgact
     1201 gaagcgcgtt cccggtgccc ccgaaccaac cattatccca aaaaatgcac cggaaacgcg
     1261 ctgccaacat tttagcctgt ctctggttac catcccagcg aagggccatc cagtgtccgt
     1321 tcctgaattt ccggctggcg ctcccgctcc agggataacg catattcgcg ctcctgtatc
     1381 agccgctcat cccttatctc cagttctcgc tgtataactg gctcaagcgt tctgtccgct
     1441 cgctcaagtg ctgcacctgc tgactcaact gcatgactcg cccgttcagc atcgcgttgt
     1501 cccgttgcgt aagcgaaaac attttctgca attccacgaa ggcgttctcc cattcgttca
     1561 gccgctgcat atagtcctgt tgcagttgct ctaatgcgtt cagcaaatgt ctttccagct
     1621 ccgtcatgct ctgtcactcc tgcaccagtc tcactgcgtt cagtttgccg ttgtccttcg
     1681 tccagttcgt ttcggctttc atccccttcg gcaacaccag aaaacgtccg ttgctgtcct
     1741 cgtgataacg cacgccccat gtcttcgcgt tcagcttctc cagactctct ttctgctcgc
     1801 ggagaatatt catattcgcg tttatcttca tgccctggta ccacagcaga ctcccggtcg
     1861 tggcaaacat cagtactgag acgataatca gccacagcca gcgtttcacg ctcattttca
     1921 gtacggtttt ccagtgcgcg tccatcgtat cgacggtcgt tttctgatac ctctccagca
     1981 gttctttcag ggctgttgta tggtcgttga tagcgttcct gattaaagat tcgtttgagg
     2041 tcagttccct gtttacagat tcttcgagct tcctgaactc gtcgttcagc attttctctg
     2101 tagattcggc tctctctttc agctttttct cgaactccgc gcccgtctgt aaaagattgc
     2161 tcataaaacg ctcctttcag tctgatattc ctcccgccgt tcgggtctgc aatgctgata
     2221 ctgcttcgcg tcaccctgac cacttcaagc cccgcctctg tgagcgcctg aatcacatcc
     2281 tgacggcctt ttatctctcc ggcatggtaa agcgcgtcta tcccgcgtgt gacgccctca
     2341 gcaagcgcct gtttcgtttc cggcaggtta tcaggaaggg tcagcgtccg gcggttctcc
     2401 ggggcgttgg ggtcatgcag accgtaatgg tgattcacca gcgtctgcca ggcatcaatt
     2461 ctcggcctgt ctgcccggtc gtagtacggc tggaggcgtt ttccggtctg tagctccatg
     2521 ttcggaatga caaaattcag ctcaagccgt cccttgtcct ggtgctccac ccacaggatg
     2581 ctgtactgat ttttctcgag accgggcatc agtacacgct caaagctcgc catcactttt
     2641 tcacgccctc caggcggcag ctccttctcc gcgaacgaca gaacacctga cgtgtatttc
     2701 ttcgcaaatg gcgtggcatc gatgagttcc cggacctctt ccggattccc ctgaagcacc
     2761 gttgcccctt cccggttacg ctcccttccc agcaggtaat caaccggacc actgccacca
     2821 ccttttcccc tggcatgaaa cttaactatc atcccgcgcc ccctgttccc tgaccgccag
     2881 acgcagccgg cgcagctcat ccccgatggc tatcagtgcg gccaccacct gaacccggtc
     2941 accggaagac cactgcccgc tgttcacctt cctcgctgtc tggttaaggt tattcccgat
     3001 agcggccagc tgacgcagta acggcggtgc cagtgtcggc agcttcccgg aacgggcaac
     3061 aggctcaccc aggcagaccc gccgcatcca tgccgccagt tgtttaccct cacagcgttc
     3121 cagtaaacgg gcatgctcat catcagtcac ccgtatcgtg agcatcctct cgcgtttcat
     3181 cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcca gtgactaaac
     3241 aggaaaaaac cgcccttaac atggtccgct ttatcagaag ccagacatta acgctgctgg
     3301 agaagctcaa cgaactggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc
     3361 acgccgatga gctttaccgc agttgcctcg cacgcttcgg ggatgacggt gaaaaccgct
     3421 gacacatgca gctcccggag acggtcacag cttgtctgtg agcggatgcc gggagcagac
     3481 aagcccgcca gggcgcgtca ggccgttgac ctttggggtg tcggggcgaa gccctgaccc
     3541 agtcacgtag cgatagcgga gtgtatactg gcttaatcat tcggcatcag tgcggattgt
     3601 atgaaaagtg cgccatgtcg ggtgtgaaat gccgcacaga tgcgtaagga gaaaatgcca
     3661 gttcaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcggctgcg
     3721 gcgagcggac tctcctttct caaaaacgct aaccctgtta tccacagaac ccggggataa
     3781 accaggaaag aacatgtgcg caaaaaacga aaccccggaa aaggccgcgc cggaggcgct
     3841 ttttccatag gctccgcccc cctgacggac ttcacaaaac agacactttt tcggtgcatc
     3901 tgtgggagcc ttgcggctca accatgaaat ccgccagcgg tggcgaaacc cgacgtggct
     3961 taaagatcat caccgtttcc ggtgaatcgc tccctcctgc gcccccacgt tcccgccata
     4021 tcgattaccg gatacctttt ccgcctttct cccttacggg acacgtggcg ctttctcata
     4081 gctcacgtct tctggtatct cggggcgact taggcagttc gctccgggct gggctgtatg
     4141 caggaacccc ccgttcagcc cgactgctgc gccttctccg gtaactgttc tcttgggtcc
     4201 aacccggaaa agacaccaca aagcgccact ggcagcagcc attggtaaaa gtcatgtgcg
     4261 agatatttag agagtacttg aagtggctgc cggagtgcgg ctacactaga agaacaattt
     4321 tggtgactcg tctcgcataa gacagttacc tggttaagca gtctgcaagc tgacttaacc
     4381 ttcgatcaaa ccgccctgca aggcggtttt ttcgtttaca ggcagcagat tacgcgcaga
     4441 aaaaaaggat ctcaagagga tcctttgatc tttttgttac aaacgtctct atttgactcc
     4501 cctgagagcg ttttagcgcg cctctgcccc ccttggttac attccgacac cagaaacgct
     4561 cccgtttttt acgtgcattt ggcacggtat cgatggaggc cggggggggc aatcctgaac
     4621 agaatgttac tcctctgagt attttctgtt aagaaaatat acacttagca cagttacaat
     4681 aaatcctacc ccctgtaaga atgtgaattg ttcatccaaa aacaaccatg acaaaaataa
     4741 tgcaactagc ggaataaagt acaacattat ccctgccaaa tgaactggga gccaacgcaa
     4801 ggagttcaag taaaaacaaa aagctaaaaa atattgtaat attccagatg aaaaaacaat
     4861 aatcacggat tccaacggaa gataaattga ggatttagat ataaaaataa agagcgatga
     4921 cagtataagg gagaatgtca actgtccgct caataatgtg ataggctcaa acgatttaat
     4981 aaggcgagac gatataacaa cataaaatgc tgccgataac acaccggcaa caacaagaca
     5041 atatccaaca atattattcg atccattact gatgtctggc aaggctacca aaatactgcc
     5101 aacaattgca cctataagga acaatataaa atattgcggg gaattaattt taactgagca
     5161 aataagaaca gacaacacca atataaacac tgattcaagt gaaaaaataa ttgatgcgct
     5221 tcctgctgaa ataaaataaa gtccgtagag tgtaaaagta tacgctaaga atggttcaaa
     5281 aacgcctgtc gcataagcaa gtatcgcttt tgagtttaat gggagttttt ttctttttat
     5341 aaaaagaact atccaagata gtattactgc actacaaatc tgccagaaaa acacccctcc
     5401 ggcatctagt tcttcaactc cttttttgct cattacgata gctgcccccc atgagaacgc
     5461 tgctaaaaaa gcaaaaagca ttcctttaat catattaatc atcctctttc tcataaaggg
     5521 ggttgttttc aaatttcctt ggttgaaagg agttaaaaaa catagatgca tcatctacag
     5581 gaacaccaat aaactctcca taataaggat catctatagg aaactcttct aatgaaacat
     5641 aaaacaatac attccagttg aagatcattg gatcactatt atctatttca tggagcaatc
     5701 tttcatcctt agttaattct cctggaatta atacatcagt atgataaaaa ccgctgtaat
     5761 aagaactatt accggtgtgc actgggcatc caaaacgatc caatttagat tctgaatgcc
     5821 atgttgtaga aatcccttcc ggatgtgaat acccaccgtt accaacagac tga
//
