LOCUS       NZ_OY754412             5872 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F467 plasmid pF467-3, complete sequence.
ACCESSION   NZ_OY754412
VERSION     NZ_OY754412.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542980
            Assembly: GCF_963575455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 09:56:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,979
            CDSs (total)                      :: 4,864
            Genes (coding)                    :: 4,605
            CDSs (with protein)               :: 4,605
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 79 of 259
            Pseudo Genes (incomplete)         :: 171 of 259
            Pseudo Genes (internal stop)      :: 52 of 259
            Pseudo Genes (multiple problems)  :: 42 of 259
            Pseudo Genes (short protein)      :: 4 of 259
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754412.1.
            Complete genomic sequence of E. coli strain F467.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F467"
                     /db_xref="taxon:562"
                     /plasmid="pF467-3"
     gene            join(5373..5872,1..367)
                     /locus_tag="R8801_RS24840"
     CDS             join(5373..5872,1..367)
                     /locus_tag="R8801_RS24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(1571..1762)
                     /locus_tag="R8801_RS24845"
                     /old_locus_tag="FGAF467_47760"
     CDS             complement(1571..1762)
                     /locus_tag="R8801_RS24845"
                     /old_locus_tag="FGAF467_47760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021539356.1"
                     /translation="MTKQEKTALNMVRFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1828..2151
                     /locus_tag="R8801_RS24850"
                     /old_locus_tag="FGAF467_47770"
     CDS             1828..2151
                     /locus_tag="R8801_RS24850"
                     /old_locus_tag="FGAF467_47770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024188420.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAAWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALIAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2360..>2791
                     /locus_tag="R8801_RS24855"
                     /pseudo=""
     CDS             2360..>2791
                     /locus_tag="R8801_RS24855"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2829..3359
                     /locus_tag="R8801_RS24860"
                     /old_locus_tag="FGAF467_47780"
     CDS             2829..3359
                     /locus_tag="R8801_RS24860"
                     /old_locus_tag="FGAF467_47780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            3366..3599
                     /locus_tag="R8801_RS24865"
                     /old_locus_tag="FGAF467_47790"
     CDS             3366..3599
                     /locus_tag="R8801_RS24865"
                     /old_locus_tag="FGAF467_47790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021520366.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4319..5371
                     /locus_tag="R8801_RS24870"
                     /old_locus_tag="FGAF467_47810"
     CDS             4319..5371
                     /locus_tag="R8801_RS24870"
                     /old_locus_tag="FGAF467_47810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021517772.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGNGGYSHPEGISTTWHSESKLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIDNSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGVPVDDASMFFNSFQPRKFE
                     NNPLYEKEDD"
                     /name="hypothetical protein"
     Typing          1396..1477
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          752..939
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          2142..3703
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.232"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcgtctcgc cttattaaat cgtttgagcc tatcacatta ttgagcggac agttgacatt
       61 ctcccttata ctgtcatcgc tctttatttt tatatctaaa tcctcaattt atcttccgtt
      121 ggaatccgtg attattgttt tttcatctgg aatattacaa tattttttag ctttttgttt
      181 ttacttgaac tccttgcgtt ggctcccagt tcatttggca gggataatgt tgtactttat
      241 tccgctagtt gcattatttt tgtcatggtt gtttttggat gaacaattca cattcttaca
      301 gggggtagga tttattgtaa ctgtgctaag tgtatatttt cttaacagaa aatactcaga
      361 ggagtaacat tctgttcagg attgcccccc cggcctccat cgataccgtg ccaaatgcac
      421 gtaaaaaacg ggagcgtttc tggtgtcgga atgtaaccaa ggggggcaga ggcgcgctaa
      481 aacgctctca ggggagtcaa atagagacgt ttgtaacaaa aagatcaaag gatcctcttg
      541 agatcctttt tttctgcgcg taatctgctg cctgtaaacg aaaaaaccgc cttgcagggc
      601 ggtttgatcg aaggttaagt cagcttgcag actgcttaac caggtaactg tcttatgcga
      661 gacgagtcac caaaattgtt cttctagtgt agccgcactc cggcagccac ttcaagtact
      721 ctctaaatat ctcgcacatg acttttacca atggctgctg ccagtggcgc tttgtggtgt
      781 cttttccggg ttggacccaa gagaacagtt accggagaag gcgcagcagt cgggctgaac
      841 ggggggttcc tgcatacagc ccagcccgga gcgaactgcc taagtcgccc cgagatacca
      901 gaagacgtga gctatgagaa agcgccacgt gtcccgtaag ggagaaaggc ggaaaaggta
      961 tccggtaatc gatatggcgg gaacgtgggg gcgcaggagg gagcgattca ccggaaacgg
     1021 tgatgatctt taagccacgt cgggtttcgc caccgctggc ggatttcatg gttgagccgc
     1081 aaggctccca cagatgcacc gaaaaagtgt ctgttttgtg aagtccgtca ggggggcgga
     1141 gcctatggaa aaagcgcctc cggcgcggcc ttttccgggg tttcgttttt tgcgcacatg
     1201 ttctttcctg gtttatcccc gggttctgtg gataacaggg ttagcgtttt tgagaaagga
     1261 gagtccgctc gccgcagccg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa
     1321 gagcgcctga actggcattt tcgccttacg catctgtgcg gcatttcaca cccgacatgg
     1381 cgcacttttc atacaatccg cactgatgcc gaatgattaa gccagtatac actccgctat
     1441 cgctacgtga ctgggtcagg gcttcgcccc gacaccccaa aggtcaacgg cctgacgcgc
     1501 cctggcgggc ttgtctgctc ccggcatccg ctcacagaca agctgtgacc gtctccgaga
     1561 gctgcatgtg tcagcggttt tcaccgtcat ccccgaagcg tgcgaggcaa ctgcggtaaa
     1621 gctcatcggc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagtt
     1681 cgttgagctt ctccagcagc gttaatgtct ggcttctgat aaagcggacc atgttaaggg
     1741 cggttttttc ctgtttagtc actggatgcc tccgtgtaag ggggatttct gttcatgggg
     1801 gtaatgatac cgatgaaacg cgagaggatg ctcacgatac gggtgactga tgatgagcat
     1861 gcccgtttac tggaacgctg tgagggtaaa caactggcgg catggatgcg gcgggtctgc
     1921 ctgggtgagc ctgttgcccg ttccgggaag ctgccgacac tggcaccgcc gttactgcgt
     1981 cagctggccg ctatcgggaa taaccttaac cagacagcga ggaaggtgaa cagcgggcag
     2041 tggtcttccg gtgaccgggt tcaggtggtg gccgcactga tagccatcgg ggatgagctg
     2101 cgccggctgc gtctggcggt cagggaacag ggggcgcggg atgatagtta agtttcatgc
     2161 caggggaaaa ggtggtggca gtggtccggt tgattacctg ctgggaaggg agcgtaaccg
     2221 ggaaggggca acggtgcttc aggggaatcc ggaagaggtc cgggaactca tcgatgccac
     2281 gccatttgcg aagaaataca cgtcaggtgt tctgtcgttc gcggagaagg agctgccgcc
     2341 tggagggcgt gaaaaagtga tggcgagctt tgagcgtgta ctgatgcccg gtctcgagaa
     2401 aaatcagtac agcatcctgt gggtggagca ccaggacaag ggacggcttg agctgaattt
     2461 tgtcattccg aacatggagc tacagaccgg aaaacgcctc cagccgtact acgaccgggc
     2521 agacaggccg agaattgatg cctggcagac gctggtgaat caccattacg gtctgcatga
     2581 ccccaacgcc ccggagaacc gccggacgct gacccttcct gataacctgc cggaaacgaa
     2641 acaggcgctt gctgagggcg tcacacgcgg gatagacgcg ctttaccatg ccggagagat
     2701 aaaaggccgt caggatgtga ttcaggcgct cacagaggcg gggcttgaag tggtcagggt
     2761 gacgcgaagc agtatcagca ttgcagaccc gaacggcggg aggaatatca gactgaaagg
     2821 agcgttttat gagcaatctt ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga
     2881 gagccgaatc tacagagaaa atgctgaacg acgagttcag gaagctcgaa gaatctgtaa
     2941 acagggaact gacctcaaac gaatctttaa tcaggaacgc tatcaacgac catacaacag
     3001 ccctgaaaga actgctggag aggtatcaga aaacgaccgt cgatacgatg gacgcgcact
     3061 ggaaaaccgt actgaaaatg agcgtgaaac gctggctgtg gctgattatc gtctcagtac
     3121 tgatgtttgc cacgaccggg agtctgctgt ggtaccaggg catgaagata aacgcgaata
     3181 tgaatattct ccgcgagcag aaagagagtc tggagaagct gaacgcgaag acatggggcg
     3241 tgcgttatca cgaggacagc aacggacgtt ttctggtgtt gccgaagggg atgaaagccg
     3301 aaacgaactg gacgaaggac aacggcaaac tgaacgcagt gagactggtg caggagtgac
     3361 agagcatgac ggagctggaa agacatttgc tgaacgcatt agagcaactg caacaggact
     3421 atatgcagcg gctgaacgaa tgggagaacg ccttcgtgga attgcagaaa atgttttcgc
     3481 ttacgcaacg ggacaacgcg atgctgaacg ggcgagtcat gcagttgagt cagcaggtgc
     3541 agcacttgag cgagcggaca gaacgcttga gccagttata cagcgagaac tggagataag
     3601 ggatgagcgg ctgatacagg agcgcgaata tgcgttatcc ctggagcggg agcgccagcc
     3661 ggaaattcag gaacggacac tggatggccc ttcgctggga tggtaaccag agacaggcta
     3721 aaatgttggc agcgcgtttc cggtgcattt tttgggataa tggttggttc gggggcaccg
     3781 ggaacgcgct tcagtcagag cttgctgaaa acacaggaac gcgttaaaac gcatgcgggc
     3841 gcgattctgg tgtgtctttg ttgctgtgtt ttgccgttat tgccagatcg cgctcattcc
     3901 catgaatttc tgtttttccg ctccggttcc gtcttctgcc tttccgaaag tatgaaaaaa
     3961 atccgtttca gtagtctctg gctggcaatt cctgcgtgat tcacatggct gcatagctat
     4021 gcatgtggtt aaaatttatc gggtgcgatc gcggcagttt ttcgggtggt ttgttgccat
     4081 ttttacctgt ctgctgccgt gatcgcaccg aacgcgtttt ggcggtgcgt acaatgcgac
     4141 gttatggtaa atggagcgga ttttcaggaa ggagtgatac taacaactcg aacactgtgc
     4201 aaataatgat tttttaaaat atcggtaacg ataataaatt atttcgtttt acgtcggtaa
     4261 taaatataat gtagtttcag tatataaaaa atatatgagg tgtcttgtgg gagctttcat
     4321 gaatcttcaa caacgtataa ataaactacc acaattatca tccagttttt catttggaaa
     4381 agatattgat aatattcatt catttatctt taacgaaact tctaaagata aaattgaaga
     4441 tttactaaga aaatgggtgt caggcaatca gccctgtgta tttggtaaat tggcaagcaa
     4501 gaaaattaaa gggcttgatt tccatttatc tattgtaaat agcccccaac tatataatga
     4561 tgatggtcat ttgtttgatt ttttgcgtaa tgaaagagtt cgttttaaag aacgcgcaag
     4621 aagaggagag gtcagtgcgc atctcattta ttttattcat ccacaacttg catttgctcg
     4681 tccatcagaa gagttagtgg acatacaaaa atatatttgt tcgctgcata tgccagagtg
     4741 ctatccaata aaagaggatg ttatttatac tgaaagcgta ccatttcagg ataaagatgg
     4801 gctaaaaata tataaggctg gtgttaatgt tttttactct tctgctcata gaacaagaaa
     4861 tcatgataga agaataccgg ggggaatact tatttctgta aatgccccgg ggcattttat
     4921 gaggctggcg atagaaaaag gtttctataa agatcaagag caagcgttag cggatattcg
     4981 taacatgact attcagtctg ttggtaacgg tgggtattca catccggaag ggatttctac
     5041 aacatggcat tcagaatcta aattggatcg ttttggatgc ccagtgcaca ccggtaatag
     5101 ttcttattac agcggttttt atcatactga tgtattaatt ccaggagaat taactaagga
     5161 tgaaagattg ctccatgaaa tagataatag tgatccaatg atcttcaact ggaatgtatt
     5221 gttttatgtt tcattagaag agtttcctat agatgatcct tattatggag agtttattgg
     5281 tgttcctgta gatgatgcat ctatgttttt taactccttt caaccaagga aatttgaaaa
     5341 caaccccctt tatgagaaag aggatgatta atatgattaa aggaatgctt tttgcttttt
     5401 tagcagcgtt ctcatggggg gcagctatcg taatgagcaa aaaaggagtt gaagaactag
     5461 atgccggagg ggtgtttttc tggcagattt gtagtgcagt aatactatct tggatagttc
     5521 tttttataaa aagaaaaaaa ctcccattaa actcaaaagc gatacttgct tatgcgacag
     5581 gcgtttttga accattctta gcgtatactt ttacactcta cggactttat tttatttcag
     5641 caggaagcgc atcaattatt ttttcacttg aatcagtgtt tatattggtg ttgtctgttc
     5701 ttatttgctc agttaaaatt aattccccgc aatattttat attgttcctt ataggtgcaa
     5761 ttgttggcag tattttggta gccttgccag acatcagtaa tggatcgaat aatattgttg
     5821 gatattgtct tgttgttgcc ggtgtgttat cggcagcatt ttatgttgtt at
//
