LOCUS       NZ_OY754405             4110 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F437 plasmid pF437-7, complete sequence.
ACCESSION   NZ_OY754405
VERSION     NZ_OY754405.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542975
            Assembly: GCF_963575435.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:03:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,023
            CDSs (total)                      :: 4,903
            Genes (coding)                    :: 4,693
            CDSs (with protein)               :: 4,693
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 85 of 210
            Pseudo Genes (incomplete)         :: 133 of 210
            Pseudo Genes (internal stop)      :: 52 of 210
            Pseudo Genes (multiple problems)  :: 53 of 210
            Pseudo Genes (short protein)      :: 2 of 210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754405.1.
            Complete genomic sequence of E. coli strain F437.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F437"
                     /db_xref="taxon:562"
                     /plasmid="pF437-7"
     gene            join(3579..4110,1..1628)
                     /gene="mobQ"
                     /locus_tag="R8858_RS25100"
                     /old_locus_tag="FGAF437_48500"
     CDS             join(3579..4110,1..1628)
                     /gene="mobQ"
                     /locus_tag="R8858_RS25100"
                     /old_locus_tag="FGAF437_48500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_052940773.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILSPATAPAWCTNRTELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLSLEIQIFRTELRNSGLTEPARRVADLLNIELPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMTDMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTEFETQWQQWGASGLAALRAQL
                     LKREEELRRKELEDTERSRAERRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMT
                     SYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQHTRNLRQPKTWPEFFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNRHLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1878..2579)
                     /locus_tag="R8858_RS25105"
                     /old_locus_tag="FGAF437_48510"
     CDS             complement(1878..2579)
                     /locus_tag="R8858_RS25105"
                     /old_locus_tag="FGAF437_48510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000227989.1"
                     /translation="MVDLIAYKSNALVEASYKLTLQEQRFLLLCISRLQSGVDAPPPEL
                     QKTMTITAVEFFNSFPDMGRNHAEEKLQEAIDRLWDRSIILKDGEKREEFRWIQYRAQY
                     LKGEAKVQITFADAVMPYLTQLKGQFTKVVIKNISNLSKSYSIRIYEILQQFRNTGERM
                     ISLDDFRSMLQLEDKYKDFKTLNRALIKPAVAELNQKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3111..3401)
                     /locus_tag="R8858_RS25110"
                     /old_locus_tag="FGAF437_48520"
     CDS             complement(3111..3401)
                     /locus_tag="R8858_RS25110"
                     /old_locus_tag="FGAF437_48520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091756.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVSRIQPLIDELNALPKPVPKGVSKNGQQA"
                     /name="hypothetical protein"
     Typing          complement(3403..3578)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="9.6e-71"
     Typing          3579..3937
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.222"
                     /coverage="100.0"
                     /evalue="3.97e-172"
     Typing          4..1625
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.227"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3493..4109
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.631"
                     /coverage="99.0"
                     /evalue="6.87e-125"
ORIGIN
        1 acgccggtta tcaggaagag atagaccatc gttcatacga gcgacagggg cttgagaaaa
       61 cgcctggtat tcacctcgga aagtctgcct gtgcgatgga aacacgcggt atcgaaacag
      121 aacgcggcga acagaatcgt ctgattaaca ggcttagcct ggaaatacag attttccgta
      181 ccgaactcag gaacagcggg ctgacagagc ctgcccgccg tgtggctgac cttctgaata
      241 ttgagcttcc tgacaacgca acttcgtaca cactacgtga catcatcgaa gcgttgccga
      301 aggacagtaa cgcggcatgg aaactcacca gtaatgccat gtcgatgatg acagatatgc
      361 aggcgacgcg cagacgctgg gaagagctga acaccgaacg aaaggaagcg atgagccatg
      421 ctgccagcct gaaaaaatca tcgccgtttt cgtccggttt ttcccgtatt cccctgatgc
      481 aatgggctgc accggaatac cgtagtgagc agggaaaaat tcagtctctg agccagcaaa
      541 tggaaaaatt gcgacagcat taccggaagg tggagaaaca ggacattccc gcgagtcaga
      601 cggaatttga aacacagtgg cagcagtggg gggcatccgg ccttgcagct ctcagagccc
      661 agttgcttaa acgtgaagag gaattgcgcc ggaaggagct ggaggataca gaacgtagtc
      721 gcgcggagcg acgagcggag cagctcagga aaaatgacaa tgccgttatg gattcagggg
      781 tactgagagc agtcgttacg gggtatggcg aggcccccat gcctggtaac ggggaaatga
      841 ccagttacct tctgcttcac aaccggaacg gagaatatac gctctggggg aacgagctgg
      901 agaaataccg caccagaatc tttgagcgtg ttgatttaat gcgtgacagg agtggttata
      961 tctgtgaccg aagcgaaatc ggtcaacatc caccattgca acgtgtttat tcctctgcga
     1021 cctttgagca actgctgacg caggtttgcc agacatggcc acaacatacc cgcaatctcc
     1081 gccagccgaa gacatggccg gagtttttct gtcttggaga agaccgccag ccagccatgc
     1141 catcactggc agcaaggaag gtggatttta cgcagggacg tctgccaccg acgctgatgc
     1201 cggtaatgag ttctgtcgac cgggaaaccc gccagcttca gcttttgctg gtcatgggcg
     1261 tggatgactc tctgggcggc gttgtccggc ttaatggcac gctgtatccc gcgtttgccg
     1321 ttccctcagc cgataacagg catctggtca tcagtgcgct gacggacaag gggttacggt
     1381 atgcagggta tggtgttgcc gtcaatcacg atgcagacag ccacatatca cctgcaccgg
     1441 aacttatgga gtttcatctg aaaacccgtg aggcaccgct ttttgctgcc gtcaatacac
     1501 cagaaaaaca gccggatcat ctgttccgct cgctgggttt taatcgtacc tgggacgaat
     1561 ggcggcgcga ggaagatgca cgaacacacg ccaccgagag gaggcacgat cgcggttggt
     1621 cacagtgaat gtgcacaata ttttgttttg tttaacatca tgttatgttc atgtgacgtt
     1681 cacatgacag ggatgaacgt caataagcac acacaacaaa ctagataatc tccgcatagt
     1741 ttcttccccc cgcatatggg ggattttttt agctcgtgag agcgattctg acgatgtttt
     1801 tgtgattcgt agtaccatcg cataccttac acaaaaaaaa cgcgtcagcg cgcgttttag
     1861 atgccttaga atcgatttta gatcgtcatc ttgatctgtt tgtcttcctt gaagcgaaaa
     1921 tgcagggcta cgacagtgcg ccccttcttg attgtctcaa ccgttacggc cagatcgctt
     1981 ttttgattca gttccgccac tgccggtttt atgagggctc tatttaacgt cttgaaatct
     2041 ttatatttat cttccagttg tagcatgctg cggaaatcat ctagagaaat catccgctcc
     2101 cctgtgttac ggaactgttg gaggatttcg tatattctga tgctataact ctttgataaa
     2161 ttagatatgt ttttaattac aacttttgtg aattgccctt ttaattgcgt taagtacggc
     2221 attacagcat ctgcgaaagt tatttgaact tttgcttcgc ctttcaggta ctgcgcccga
     2281 tactggatcc aacggaactc ctcgcgtttt tcaccatcct taagaatgat cgacctgtcc
     2341 cacagacgat cgatcgcttc ctgtaacttt tcttcggcat ggtttcttcc catgtcagga
     2401 aatgaattaa aaaactccac agccgtaata gtcatggtct tttgtaattc aggtggagga
     2461 gcatcaacac ctgactgtaa tcgactaatg cacagcaaca ggaaacgctg ctcctgtaac
     2521 gtgagcttgt agctggcttc gactaaggca ttggatttgt aagctatcaa atcaaccatt
     2581 gagcaccctc acctaaagta aactaaaaaa aaactagttc accccatcaa ttggtgcaat
     2641 catttttttt taattcacca ttttctgtgg ataaccaccc ccgaaatgtt cacctttacc
     2701 cccgaaatgt tcacctaacc acccccgaaa tgttcacctt tacccccgaa atgttcacct
     2761 atagcaatct aatcccttga attccataag aaatttcggt ccgaaaacaa gtattaaaaa
     2821 caagatctta aaaacaagat ctaaaaactt aacggcgagc ctgtggaaaa ctcggggtgc
     2881 gcaaaacaaa aacccatgaa aaacagtaag ttacaaaaaa gcagaacagc agacactggc
     2941 aaaacgctgt tcaaccctgt ggaaaaccag tattcatgcg ggttgaacgg ctatcaccaa
     3001 ctgagcatcg gcgcgtaagt gccctgtttt ggtgcagcag cttttccgct tcctcgccca
     3061 ctcgctcgct aacgctcggt cgctggggct gcggcgagcg gatggtgcat tcacgcctgc
     3121 tggccgtttt ttgacacgcc tttggggacg ggttttggca acgcgttcag ttcgtcaatc
     3181 aacggctgta tcctgctcac atcagcctgc cgtgtcacct ctgccttcag gaactggagt
     3241 aaccaggcgc gcaattgcac ctcctccggc tttctgacct ttgccagcaa tgcagcacct
     3301 acaacgacct tctgcgctgt gtccaggctc cggctctttg ccttcagacg ctgcaatctg
     3361 gcttcagctt cggcaatttt ctgttcgagt gttctgctca tgatgtaact ccgtgcgcgg
     3421 tgaaaaatcg cattttagct cgtcactggt agtttaaaaa ctgagctggc ataatgcgca
     3481 gaaatcacga agtgcgcact tatacaatct caccttcggt tcgattgtgt gcgccctgcg
     3541 gggctgaaag aaaactgcaa aaaggcatta cggcagaaat ggcgatttat catctcagca
     3601 tgaaaatcat ctccagaagc aacggctaca gcgccgttgc ttctgctgcc tatcgttccg
     3661 gttcactaat gcttgatgag cgtaccggac tgactcacga ctacacccgc aaaagcggtg
     3721 tggctgaggc tgtgattctg tcccccgcca ccgcccctgc ctggtgtacg aaccgtaccg
     3781 aactctggaa tgccgttgag aaggccgaac gtcggaagaa ctcacaactt gcgcgggaaa
     3841 ttgaactcgc catcccccgt gaactgccgc aggatgccgc acgggaaacc gttctcgcct
     3901 ttgtcagaga aaactttgtc agtcagggca tgattgctga tgtggcgttc catcacatgg
     3961 ataaaacaaa tccccatgcg cacatcatgc tgaccacgag ggctgtcgga cctgcggggt
     4021 tcggtggaaa ggtcagggac tggaacgacc ggacacatgc cgagacgtgg cgggcatcat
     4081 gggctgacca tgcaaacagg gcactggcga
//
