LOCUS       NZ_OY754404             5463 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F437 plasmid pF437-6, complete sequence.
ACCESSION   NZ_OY754404
VERSION     NZ_OY754404.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542975
            Assembly: GCF_963575435.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:03:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,023
            CDSs (total)                      :: 4,903
            Genes (coding)                    :: 4,693
            CDSs (with protein)               :: 4,693
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 85 of 210
            Pseudo Genes (incomplete)         :: 133 of 210
            Pseudo Genes (internal stop)      :: 52 of 210
            Pseudo Genes (multiple problems)  :: 53 of 210
            Pseudo Genes (short protein)      :: 2 of 210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754404.1.
            Complete genomic sequence of E. coli strain F437.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F437"
                     /db_xref="taxon:562"
                     /plasmid="pF437-6"
     gene            join(5283..5463,1..353)
                     /locus_tag="R8858_RS25075"
     CDS             join(5283..5463,1..353)
                     /locus_tag="R8858_RS25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168774.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQNLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVILED
                     KLAEKPVTGKNRKN"
                     /name="hypothetical protein"
     gene            574..1698
                     /locus_tag="R8858_RS25080"
                     /old_locus_tag="FGAF437_48450"
     CDS             574..1698
                     /locus_tag="R8858_RS25080"
                     /old_locus_tag="FGAF437_48450"
                     /inference="COORDINATES: protein motif:HMM:NF013211.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_021529456.1"
                     /translation="MSGGDGRGPGNTGLGHNGGKPSGNVNGSSGHGGPSSGGGYHYNPG
                     KGPASTVGPDGEVHINITGGMEKDPPGKHHGNGGSGNGHGAGNHNSNSGGNGKTTSIPG
                     WSPDSGKPGRYLPTNKDKLIKGLIQSIDDHSGTYTIHYTGGAVATVTVPNGDVNKMNVH
                     YNIGSGPSSGNKLTRDTIKSFLEFKAENEANAVNDAISKVNDFYKELASKYGDKLEKEA
                     KALADEAKGKKIRNANEAIKAFDRYKDVLNKKFSVSDREAIAKALESLNKDQMAKQLKI
                     FGKAFGVVGEAIQWGGFISGLVKGFRTGDWNEAFISGEKIAVGKVASVMVTVAFSAMAV
                     NPIGILGFAVIMAVTSALITDERLKQLNSFINGI"
                     /name="colicin-like pore-forming protein"
     gene            2499..3518
                     /locus_tag="R8858_RS25085"
                     /old_locus_tag="FGAF437_48460"
     CDS             2499..3518
                     /locus_tag="R8858_RS25085"
                     /old_locus_tag="FGAF437_48460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271364.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(3779..4177)
                     /locus_tag="R8858_RS25090"
                     /old_locus_tag="FGAF437_48470"
     CDS             complement(3779..4177)
                     /locus_tag="R8858_RS25090"
                     /old_locus_tag="FGAF437_48470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            4354..5280
                     /locus_tag="R8858_RS25095"
                     /old_locus_tag="FGAF437_48480"
     CDS             4354..5280
                     /locus_tag="R8858_RS25095"
                     /old_locus_tag="FGAF437_48480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001736644.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKNGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIMEWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     Typing          2500..3274
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          4355..5277
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggtgcagaa tctggacgta gcagggctga taggtgagcg catcagccag gattttcagc
       61 ggctggaaga gtcagtcgca aacatgaagg caaacgcgga tgccctgaac agggatattg
      121 cgacaattca cagcaatatc gtgcaggaat acaacaccct taaagggtat agctggaaat
      181 ggctgggtgg ggtgtcagtg gcttttctag ttatgttctg tgtgctcgga tggggaatga
      241 aaacaatgct cgacaggaac tacaacgcca cagcggcggt gtatcagaaa gttgtgatac
      301 tggaagataa actggcggaa aaacctgtca cgggtaagaa ccggaaaaac tgacgcgctt
      361 cgcttgtcca acgcctcacg gacagcgaaa ggatttcgcg ttccgttcac cgttttcatg
      421 agtgattcct ccgaactgcc aaaaaatatc cctccctgac ttgacagaaa aaataatggc
      481 gagtactttt tgatctgtac ataaaaccag tggttttatg tacagtatta actatgtaat
      541 taattgtttt aacgattaaa agagggaatt tttatgagtg gtggagatgg tcgtggccct
      601 ggtaatacag ggcttggaca taacggtggt aaacccagtg gaaacgtgaa tggctcatct
      661 ggacatggag gaccatcatc tggcggtggg tatcattaca atccgggaaa aggtcctgca
      721 agcactgtag gtcctgacgg ggaggtgcat atcaacatta ccggtggtat ggaaaaagat
      781 cctccaggaa aacatcacgg taatggtggc tcaggtaatg gtcacggtgc tggaaatcat
      841 aacagcaatt caggcggcaa tggaaaaaca acgtctattc ctggatggag ccctgactct
      901 gggaaacctg gcagatattt accgacaaat aaagataagt taattaaggg acttattcag
      961 tcaatagatg accattctgg aacgtacact atccattata ctggtggtgc agtagctaca
     1021 gtaactgtac caaacggtga tgtgaataaa atgaatgttc actataatat cggtagtggg
     1081 ccgtcatcag gtaataaact gactcgtgat actataaaaa gctttttaga attcaaggca
     1141 gaaaatgaag ctaatgctgt aaatgatgca ataagcaaag ttaatgactt ttataaagag
     1201 ttagcatcaa aatacggcga taagttggag aaagaagcca aggcactagc cgatgaagct
     1261 aaagggaaaa aaattcgcaa tgctaatgag gcgattaaag cctttgatag gtacaaggat
     1321 gtgcttaata agaaattcag cgtatccgac cgagaagcga ttgcaaaagc actggagtct
     1381 cttaataaag atcaaatggc taaacagtta aaaatttttg gtaaggcctt tggtgtcgta
     1441 ggtgaggcta ttcagtgggg gggatttatc agtggcttag ttaaaggatt cagaactggt
     1501 gactggaatg aggcttttat aagcggggaa aaaatcgcag tcggcaaggt agcatctgtt
     1561 atggttacag tggcatttag tgctatggca gtaaatccta ttggtatttt agggtttgca
     1621 gtaattatgg cagtgactag tgccttaatt acagatgaaa ggctgaaaca actaaacagc
     1681 tttattaacg gcatttaata aacaaaaggt cgtaccgttt gatatggtat gacctttatt
     1741 ttgtttttct atcgcttttt gttggttata aagtaataga ctgtaaaaat caatccaaat
     1801 ggaggtagtg ataatataat gcatattata aaaccccagt taacgaagtt aaaagttgtt
     1861 tttgttagta agggtgcttg cttatttatt agtaatagac aaaaagggta tgtggacgat
     1921 ccaaaaagag atataagtat aaatataaat aattctatac ttgttgggct tagattcaaa
     1981 cttgcgatga taaaagataa aatgtaaaag gataaggcaa ggataaaatt ccttaaatag
     2041 aattccatat ctattttttt catttataaa atcctcgtta gttaaatgtt tataatattg
     2101 ccttacttta tccataaatc tagaagtagt tatcacaaaa gttacttttt tgactcgttc
     2161 gcccttttga acggtcgaaa ctgtttgcga atcgctttcg tgcatagtta tgcaacgccc
     2221 tgaaagtgat tctgacgcat ttttctggtt tggcctggtg tttccttgtc tttttgcgtt
     2281 ttttgcgcca gaaagcgact gagggcgttt taaggggtac gtacaacggg agttatggta
     2341 aatggatcgg ttttgcggga aggctcgaca ggttttgttg ttgggggtag tgtaagcgac
     2401 tgaaaaacaa acgcccctga aattcgtgtc tctccgccaa gattgtcacg agaatacagg
     2461 ggcgaggtcg gtcgataacc tggaaggaat cgtaacacat gagcgccgcg cttcaatact
     2521 tcgaagaaaa tttaccccac cgcccctatc acacggatga tctcgctttc ggccttcgca
     2581 tctccggcaa agggcgtgct cttcttgcgc ggtacatcca gcagaaccag cctcatgcgc
     2641 agttctggct ggtttttgac gttgaccgcg agggggctgc gattgactgg agcgaccgga
     2701 acgcccccgc gccgaacatc accgttaaaa atcctgtgaa cggacacgct cacctgctct
     2761 acgcactcaa catcgccgtg agaaccgcgc ctgatgcctc ggttaaggcg ctgaaatatg
     2821 ccgcagcggt tgaacgttcg ctgtgtgaga aactttgtgc tgatgtgaac tacagcggcc
     2881 tgatttgcaa aaatccgttc cacctggaat ggcaggtgat ggagtggcgc gaggacgcct
     2941 ataccctcga tgaactggca gattatctcg atttgagcgc ctcagagcgt cgtagcatcg
     3001 ataaacatta cgggatgggg cgaaactgcc acctgttcga aatgacgcgc aaatgggctt
     3061 acagggcgat tcgtcagggc tggccagcgt tctcacagtg gcttgaagcg gtgatccagc
     3121 gtgtcgaaat gtacaatgca tcgcttcccg ttccgctttc actggctgaa tgtcgggcta
     3181 ttggcaagag cattgcgaaa tacacgcaca ggaacttcac gccggaaact ttcgcacagt
     3241 atgtggctga tacgcacacg ccagaaattc aggcagcacg cggtcgcaag ggcgggaagg
     3301 ccaattcgtc agaaaatcag tcggacaaag ggaaaaaaag cgccgctgtt cggtggactg
     3361 ctaacgacga caaacgtcgt agggcgcttg atatgtatat cttgggagcg tcaacggaag
     3421 atattgcagt ggcggttggt gttagcagcc ggactattcg acgctggatg gataattctg
     3481 gagaatggct aaccaagaaa caaataatta aatgttagtt aacaaagaag caacactacc
     3541 ccccgttttt tgttaatggg atgttaaatt cgcggacatg aacaagccta tatcagataa
     3601 cagcgccttt cggcgtcttt ttgagcagcg ggctttccgg cggttttctc ctctcagccc
     3661 agcaatggtg cggcttaccg catgattgag gtggtcgtgc tcgccgcagt ctcatgaccg
     3721 agcgtagcga gcgaatgagc gaggaagcac cagggaaccg gaacacggat ggaggcactt
     3781 acgcgtctgg gtttggggat gtctccagat cgtacttaat ttccttaagt gcctgtaata
     3841 tcctgtcttt tttatagcat agctcgcctg tttttgtgtc taccagtcct gcttcgatca
     3901 gtagcccagc cgcgagaaag agtcggtgat tgcgttctcg tcgttgtttt tttccctcga
     3961 ttcgtttttg gaccgcctgt tttcgttcgc tctcctgtgc ctgtaagtcg tttttttccg
     4021 ctgttgccag tacgtggagg agtttgcgtt cctgttctgt gagtcctgct tcgttgtcga
     4081 gcaggtcaat aatctgtctc tgtcgtgcgg tctgttctcc gagcttcctg atgaatccga
     4141 tccgtttctt cagccattcg atgttgctga tgcgcatgtt cgtggtgctc cctggtgttt
     4201 ttttgcggtg tgttgtggtc gtgcagtata tcgcttgcgg tcggcgggtg cagggggtat
     4261 cttccgtgct aattcgtaaa attagaagga agcgcactta tatgtttctc tccttcgtcg
     4321 agaaacagtg cgctcctgcg gagggctgaa accgtggcga cctatcacct cagcgttaag
     4381 tttggtggca agggacaggc tgcaaaccat gccgattaca tcgagcgcaa agagaaatac
     4441 cgcgaccgtc aagatttgga atattccgca cacggaaaca tgccggaatg ggcgcgagat
     4501 aatccctccc atttctggca ggcggctgac cagtttgaac gtgcaaacgg ttcgacttat
     4561 cgggaactgg agatagcgct gccccgtgaa ctgacaccgg aacagcgcct tgaactggtg
     4621 caggattttg tacgtcagga agctggcgaa cggcatgcat ggagttttgc cattcacaat
     4681 ccgaaagcga gcattgaggg aggcgaacag cctcatgcgc atatcatgat gagtcagcgt
     4741 gtgaatgatg gcattgaacg tacaccggaa cagtatttcc ggcgctataa cgccaggtat
     4801 cccgagcggg gaggtgcgaa aaaggattca ggaagcctga ctcccactca gcaaaaagag
     4861 cagttgcggg aattgcgaaa acgctgggag gtgaagcaca acgaacatat gagaaaaaat
     4921 ggctttgaaa ggggcttcat tgactgtcgg acgctgaaag aacagggcat tgaacgacgt
     4981 cccgaagtgc atctgggttt tgaagctgcc ggacggcttg atgatgccca gcgtcatgac
     5041 atcatgcaaa aacggtctga accggactat tcccgccatc ttgaacaggc gcgaacggtc
     5101 gggcatgaca tcatggaatg gctggacact ccggttaagc aacatgaact ggtcagaagg
     5161 ctgcaacatg atgttgcgcg ggagcatggc tttgacatga gagacagagt gagcagacag
     5221 gaccgaactg aacctgaacg ggttaaatca ccgtcacgtg gattttctat gggattctga
     5281 acatggccag cacagaagaa aaattactca cgctcctgtt atcggtggag catttgcaac
     5341 ataccgcaat ggagcaacag aaagctgttg ctgattctct ggctgtggca agggaaacac
     5401 ttcgcggata tcgggatatt tctgacggtg tggcaaaaca ggtcagggat gaagtcagaa
     5461 atg
//
