LOCUS       NZ_OY754403             5592 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F437 plasmid pF437-5, complete sequence.
ACCESSION   NZ_OY754403
VERSION     NZ_OY754403.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542975
            Assembly: GCF_963575435.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:03:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,023
            CDSs (total)                      :: 4,903
            Genes (coding)                    :: 4,693
            CDSs (with protein)               :: 4,693
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 85 of 210
            Pseudo Genes (incomplete)         :: 133 of 210
            Pseudo Genes (internal stop)      :: 52 of 210
            Pseudo Genes (multiple problems)  :: 53 of 210
            Pseudo Genes (short protein)      :: 2 of 210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754403.1.
            Complete genomic sequence of E. coli strain F437.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F437"
                     /db_xref="taxon:562"
                     /plasmid="pF437-5"
     gene            join(5133..5592,1..1700)
                     /gene="mobQ"
                     /locus_tag="R8858_RS25030"
                     /old_locus_tag="FGAF437_48350"
     CDS             join(5133..5592,1..1700)
                     /gene="mobQ"
                     /locus_tag="R8858_RS25030"
                     /old_locus_tag="FGAF437_48350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_052940773.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILSPATAPAWCTNRTELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLSLEIQIFRTELRNSGLTEPARRVADLLNIELPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMTDMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTEFETQWQQWGASGLAALRAQL
                     LKREEELRRKELEDTERSRAERRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMT
                     SYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQHTRNLRQPKTWPEFFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNRHLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1950..>2252)
                     /locus_tag="R8858_RS25035"
                     /old_locus_tag="FGAF437_48360"
                     /pseudo=""
     CDS             complement(1950..>2252)
                     /locus_tag="R8858_RS25035"
                     /old_locus_tag="FGAF437_48360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666799.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            <2239..2334
                     /locus_tag="R8858_RS25040"
                     /pseudo=""
     CDS             <2239..2334
                     /locus_tag="R8858_RS25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /name="bacteriocin immunity protein"
     gene            2396..2539
                     /locus_tag="R8858_RS25045"
                     /old_locus_tag="FGAF437_48370"
     CDS             2396..2539
                     /locus_tag="R8858_RS25045"
                     /old_locus_tag="FGAF437_48370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            <2992..3144
                     /locus_tag="R8858_RS25050"
                     /pseudo=""
     CDS             <2992..3144
                     /locus_tag="R8858_RS25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            3154..3411
                     /locus_tag="R8858_RS25055"
                     /old_locus_tag="FGAF437_48390"
     CDS             3154..3411
                     /locus_tag="R8858_RS25055"
                     /old_locus_tag="FGAF437_48390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_000523347.1"
                     /translation="MGLKLHINWFDKTTEEFKGGEYSKDFGDDGSVIERLGMPLKDNIN
                     NGWFDVIAEWVPLLQPYFNHQIDISGNEYFVSFDYRDGDW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3431..3697
                     /locus_tag="R8858_RS25060"
                     /old_locus_tag="FGAF437_48400"
     CDS             3431..3697
                     /locus_tag="R8858_RS25060"
                     /old_locus_tag="FGAF437_48400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000861936.1"
                     /translation="MKVELKKSISDYTEAEFLELVKKICRAEGATEEDDNKLVREFERL
                     TEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            3759..3902
                     /locus_tag="R8858_RS25065"
                     /old_locus_tag="FGAF437_48410"
     CDS             3759..3902
                     /locus_tag="R8858_RS25065"
                     /old_locus_tag="FGAF437_48410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            4398..>4877
                     /locus_tag="R8858_RS25070"
                     /old_locus_tag="FGAF437_48420"
                     /pseudo=""
     CDS             4398..>4877
                     /locus_tag="R8858_RS25070"
                     /old_locus_tag="FGAF437_48420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(4957..5132)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="9.6e-71"
     Typing          5133..5491
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.222"
                     /coverage="100.0"
                     /evalue="3.97e-172"
     Typing          4..1697
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.345"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 actggaacga ccggacacat gccgagacgt ggcgggcatc atgggctgac catgcaaaca
       61 gggcactggc gaacgccggt tatcaggaag agatagacca tcgttcatac gagcgacagg
      121 ggcttgagaa aacgcctggt attcacctcg gaaagtctgc ctgtgcgatg gaaacacgcg
      181 gtatcgaaac agaacgcggc gaacagaatc gtctgattaa caggcttagc ctggaaatac
      241 agattttccg taccgaactc aggaacagcg ggctgacaga gcctgcccgc cgtgtggctg
      301 accttctgaa tattgagctt cctgacaacg caacttcgta cacactacgt gacatcatcg
      361 aagcgttgcc gaaggacagt aacgcggcat ggaaactcac cagtaatgcc atgtcgatga
      421 tgacagatat gcaggcgacg cgcagacgct gggaagagct gaacaccgaa cgaaaggaag
      481 cgatgagcca tgctgccagc ctgaaaaaat catcgccgtt ttcgtccggt ttttcccgta
      541 ttcccctgat gcaatgggct gcaccggaat accgtagtga gcagggaaaa attcagtctc
      601 tgagccagca aatggaaaaa ttgcgacagc attaccggaa ggtggagaaa caggacattc
      661 ccgcgagtca gacggaattt gaaacacagt ggcagcagtg gggggcatcc ggccttgcag
      721 ctctcagagc ccagttgctt aaacgtgaag aggaattgcg ccggaaggag ctggaggata
      781 cagaacgtag tcgcgcggag cgacgagcgg agcagctcag gaaaaatgac aatgccgtta
      841 tggattcagg ggtactgaga gcagtcgtta cggggtatgg cgaggccccc atgcctggta
      901 acggggaaat gaccagttac cttctgcttc acaaccggaa cggagaatat acgctctggg
      961 ggaacgagct ggagaaatac cgcaccagaa tctttgagcg tgttgattta atgcgtgaca
     1021 ggagtggtta tatctgtgac cgaagcgaaa tcggtcaaca tccaccattg caacgtgttt
     1081 attcctctgc gacctttgag caactgctga cgcaggtttg ccagacatgg ccacaacata
     1141 cccgcaatct ccgccagccg aagacatggc cggagttttt ctgtcttgga gaagaccgcc
     1201 agccagccat gccatcactg gcagcaagga aggtggattt tacgcaggga cgtctgccac
     1261 cgacgctgat gccggtaatg agttctgtcg accgggaaac ccgccagctt cagcttttgc
     1321 tggtcatggg cgtggatgac tctctgggcg gcgttgtccg gcttaatggc acgctgtatc
     1381 ccgcgtttgc cgttccctca gccgataaca ggcatctggt catcagtgcg ctgacggaca
     1441 aggggttacg gtatgcaggg tatggtgttg ccgtcaatca cgatgcagac agccacatat
     1501 cacctgcacc ggaacttatg gagtttcatc tgaaaacccg tgaggcaccg ctttttgctg
     1561 ccgtcaatac accagaaaaa cagccggatc atctgttccg ctcgctgggt tttaatcgta
     1621 cctgggacga atggcggcgc gaggaagatg cacgaacaca cgccaccgag aggaggcacg
     1681 atcgcggttg gtcacagtga atgtgcacaa tattttgttt tgtttaacat catgttatgt
     1741 tcatgtgacg ttcacatgac agggatgaac gtcaataagc acacacaaca aactagataa
     1801 tctccgcata gtttcttccc cccgcatatg ggggattttt ttagctcgtg agagcgattc
     1861 tgacgatgtt tttgtgattc gtagtaccat cgcatacctt acacaaaaaa aacgcgtcag
     1921 cgcgcgtttt agatgcctta gaatcgattt tagatcgtca tcttgatctg tttgtcttcc
     1981 ttgaagcgaa aatgcagggc tacgacagtg cgccccttct tgattgtctc aaccgttacg
     2041 gccagatcgc ttttttgatt cagttccgcc actgccggtt ttatgagggc tctatttaac
     2101 gtcttgaaat ctttatattt atcttccagt tgtagcatgc tgcggaaatc atctagagaa
     2161 atcatccgct cccctgtgtt acggaactgt tggaggattt cgtatattct gatgctataa
     2221 ctctttgata aattagatcc agaaggtaat aatgatggta gccctgaagc tgttattaaa
     2281 gagattaaag aatggcgagc tgctaacggt aagtcaggat ttaaacaggg ctgaaatatg
     2341 aatgccagtt gtttaaggat gaatgactgg cattctttca caacaaggag tcgttatgaa
     2401 aaaaataaca gggattattt tattgcttct tgcagtcatt attctggctg catgtcaggc
     2461 aaactatatc cgggatgttc agggcgggac cgtatctccg tcatcaacag ctgaagtgac
     2521 cggattagca acgcagtaac ccgaaatcct ctttgacaaa aacaaagcgt gtcaggctga
     2581 ttctgatgcg ctttttttga aatgtcacaa aagttccatg tgggagatgg gatctaaaat
     2641 ccctgtgcag aaccttccat ccggtgggag aaaacttgtc attttgacct gttcgccatt
     2701 cggggggggc gcttggaaaa cggaaatttt cccacgctaa gagaattgag ttgaacgagt
     2761 ggttacatct aggccagcta actcactgaa cgtaaaggct gtacgaaatg ttccgaccgt
     2821 ccgataacca tttggcttac ttccgtacat ttccttgcgt agatgtacag aattgtacga
     2881 tttcggacac caaaaccgct gttgtcccgc cattctggcg attcctgaaa gttatttgac
     2941 taaaaaatag tcttaacgtg gtttattttc cgaattccaa gcggacccca taaaggccgt
     3001 aagatttatg aatgggactc ccagcacggt gaacttgaag ggtatcgtgc cagtgatggt
     3061 cagcatcttg gctcattcga tcctaagact ggtaatcagt tgaaaggacc tgatccaaaa
     3121 cgaaatatca aaaagtatct ttgagaggat gttatggggc ttaaattaca tattaattgg
     3181 tttgataaga cgaccgagga atttaaaggt ggtgagtatt caaaagattt cggtgatgat
     3241 ggctcggtca ttgaacgtct tggaatgcct ttaaaagata atatcaataa tggttggttt
     3301 gatgttatag ctgaatgggt acctttgcta caaccatact ttaatcatca aattgatatt
     3361 tccggtaatg agtattttgt ttcatttgat tatcgtgatg gtgattggtg atcaaatatt
     3421 ctcaggagag atgaaggtgg agctaaagaa aagtattagt gattataccg aggctgaatt
     3481 tctggagctt gtaaaaaaaa tatgtagagc tgaaggtgct actgaagagg atgacaataa
     3541 attagtgaga gagtttgagc gattaactga gcacccagat ggttcagatc tgatttatta
     3601 tcctcgcgat gacagggaag atagtcctga agggattgtc aaggaaatta aagaatggcg
     3661 agctgctaac ggtaagtcag gatttaaaca gggctgaaat atgaatgccg gttgtttatg
     3721 gatgaatggc tggcattctt tcacaacaag gagtcgttat gaaaaaaata acagggatta
     3781 ttttattgct tcttgcagtc attattctgt ctgcatgtca ggcaaactat atccgggatg
     3841 ttcagggcgg gaccgtatct ccgtcatcaa cagctgaagt gaccggatta gcaacgcagt
     3901 aacccgaaat cctctttgac aaaaacaaag cgtgtcaggc tgattctgat gcgctttttt
     3961 tttgaaatgt cacaaaaatt ccatgtggga gatgggatct aaaatcctcg tgcagaactt
     4021 tccatccagg gggagaaaac ttgtcgtttt gagccgttcg gtgttcagaa cgcacgaaac
     4081 cgatcgcgca tcgctttcgt gaatagttat gcaggcccct gaaaacgatt ctgacgcgtt
     4141 ttttcggttt tgcctggtgt tttcctgtct ttttgcgttt tttgcgtcag aacgcgtctg
     4201 agggcgtttt aaggggtgcg tacaacggga gttatggtaa atggatcggg ttttcgggaa
     4261 ggttcgacag gatttgccgt tgggtgtagt gtaagcgact gaaaaacaaa cgcccctgaa
     4321 attcgtgtct ctccgccaag attgtcacga gaatacaggg gcgaggtcgg tcgataacct
     4381 ggaaggaatc gtaacacatg agcgccgcgc ttcaatactt cgaagaaaat ttaccccacc
     4441 gtccctatca cacggatgat ctcgcttttg gtcttcgcat ctctggcaaa gggcgtgcgc
     4501 ttcttgcgcg gtacatccag cagaaccagc ctcatgcgca gttctggctg gtttttgacg
     4561 ttgaccgcga gggggctgcg attgactgga gcgaccggaa cgcccccgcg ccgaacatca
     4621 ccgtaaaaaa tcccgtgaac ggtcatgctc acctgctcta tgcactcaac atcgccgtga
     4681 gaaccgcgcc tgatgcgtcg gttaaggcac tgaagtacgc tgccgcgata gagcgtgcgc
     4741 tgtgtgagaa acttggtgca gatgtgaact acagcggact gatttgcaaa aatccgttcc
     4801 acctggaatg gctggtgatg gagtggcgcg aggaagccta taccctcgat gaactggctg
     4861 attatctcga tttgagtgtc caggctccgg ctctttgcct tcagacgctg caatctggct
     4921 tcagctgcaa ttttctgttc gagtgttctg ctcatgatgt aactccgtgc gcggtgaaaa
     4981 atcgcatttt agctcgtcac tggtagttta aaaactgagc tggcataatg cgcagaaatc
     5041 acgaagtgcg cacttataca atctcacctt cggttcgatt gtgtgcgccc tgcggggctg
     5101 aaagaaaact gcaaaaaggc attacggcag aaatggcgat ttatcatctc agcatgaaaa
     5161 tcatctccag aagcaacggc tacagcgccg ttgcttctgc tgcctatcgt tccggttcac
     5221 taatgcttga tgagcgtacc ggactgactc acgactacac ccgcaaaagc ggtgtggctg
     5281 aggctgtgat tctgtccccc gccaccgccc ctgcctggtg tacgaaccgt accgaactct
     5341 ggaatgccgt tgagaaggcc gaacgtcgga agaactcaca acttgcgcgg gaaattgaac
     5401 tcgccatccc ccgtgaactg ccgcaggatg ccgcacggga aaccgttctc gcctttgtca
     5461 gagaaaactt tgtcagtcag ggcatgattg ctgatgtggc gttccatcac atggataaaa
     5521 caaatcccca tgcgcacatc atgctgacca cgagggctgt cggacctgcg gggttcggtg
     5581 gaaaggtcag gg
//
