LOCUS       NZ_OY754402             5872 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F437 plasmid pF437-4, complete sequence.
ACCESSION   NZ_OY754402
VERSION     NZ_OY754402.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542975
            Assembly: GCF_963575435.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:03:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,023
            CDSs (total)                      :: 4,903
            Genes (coding)                    :: 4,693
            CDSs (with protein)               :: 4,693
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 85 of 210
            Pseudo Genes (incomplete)         :: 133 of 210
            Pseudo Genes (internal stop)      :: 52 of 210
            Pseudo Genes (multiple problems)  :: 53 of 210
            Pseudo Genes (short protein)      :: 2 of 210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754402.1.
            Complete genomic sequence of E. coli strain F437.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F437"
                     /db_xref="taxon:562"
                     /plasmid="pF437-4"
     gene            371..901
                     /locus_tag="R8858_RS25000"
                     /old_locus_tag="FGAF437_48270"
     CDS             371..901
                     /locus_tag="R8858_RS25000"
                     /old_locus_tag="FGAF437_48270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            908..1141
                     /locus_tag="R8858_RS25005"
                     /old_locus_tag="FGAF437_48280"
     CDS             908..1141
                     /locus_tag="R8858_RS25005"
                     /old_locus_tag="FGAF437_48280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021520366.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1861..2913
                     /locus_tag="R8858_RS25010"
                     /old_locus_tag="FGAF437_48300"
     CDS             1861..2913
                     /locus_tag="R8858_RS25010"
                     /old_locus_tag="FGAF437_48300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021517772.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGNGGYSHPEGISTTWHSESKLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIDNSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGVPVDDASMFFNSFQPRKFE
                     NNPLYEKEDD"
                     /name="hypothetical protein"
     gene            2915..3781
                     /locus_tag="R8858_RS25015"
                     /old_locus_tag="FGAF437_48310"
     CDS             2915..3781
                     /locus_tag="R8858_RS25015"
                     /old_locus_tag="FGAF437_48310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(4985..5176)
                     /locus_tag="R8858_RS25020"
                     /old_locus_tag="FGAF437_48320"
     CDS             complement(4985..5176)
                     /locus_tag="R8858_RS25020"
                     /old_locus_tag="FGAF437_48320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021539356.1"
                     /translation="MTKQEKTALNMVRFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5227..5565
                     /locus_tag="R8858_RS25025"
                     /old_locus_tag="FGAF437_48330"
     CDS             5227..5565
                     /locus_tag="R8858_RS25025"
                     /old_locus_tag="FGAF437_48330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_317737945.1"
                     /translation="MKRERMLTIRVTDDEHARLLERCEGKQLAAWMRRVCLGEPVARSG
                     KLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALIAIGDELRRLRLAVR
                     EQGARDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4810..4891
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          4166..4353
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          2..1245
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.036"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5210..5562
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.763"
                     /coverage="100.0"
                     /evalue="3.9399999999999985e-68"
ORIGIN
        1 tttgtcattc cgaacatgga gctacagacc ggaaaacgcc tccagccgta ctacgaccgg
       61 gcagacaggc cgagaattga tgcctggcag acgctggtga atcaccatta cggtctgcat
      121 gaccccaacg ccccggagaa ccgccggacg ctgacccttc ctgataacct gccggaaacg
      181 aaacaggcgc ttgctgaggg cgtcacacgc gggatagacg cgctttacca tgccggagag
      241 ataaaaggcc gtcaggatgt gattcaggcg ctcacagagg cggggcttga agtggtcagg
      301 gtgacgcgaa gcagtatcag cattgcagac ccgaacggcg ggaggaatat cagactgaaa
      361 ggagcgtttt atgagcaatc ttttacagac gggcgcggag ttcgagaaaa agctgaaaga
      421 gagagccgaa tctacagaga aaatgctgaa cgacgagttc aggaagctcg aagaatctgt
      481 aaacagggaa ctgacctcaa acgaatcttt aatcaggaac gctatcaacg accatacaac
      541 agccctgaaa gaactgctgg agaggtatca gaaaacgacc gtcgatacga tggacgcgca
      601 ctggaaaacc gtactgaaaa tgagcgtgaa acgctggctg tggctgatta tcgtctcagt
      661 actgatgttt gccacgaccg ggagtctgct gtggtaccag ggcatgaaga taaacgcgaa
      721 tatgaatatt ctccgcgagc agaaagagag tctggagaag ctgaacgcga agacatgggg
      781 cgtgcgttat cacgaggaca gcaacggacg ttttctggtg ttgccgaagg ggatgaaagc
      841 cgaaacgaac tggacgaagg acaacggcaa actgaacgca gtgagactgg tgcaggagtg
      901 acagagcatg acggagctgg aaagacattt gctgaacgca ttagagcaac tgcaacagga
      961 ctatatgcag cggctgaacg aatgggagaa cgccttcgtg gaattgcaga aaatgttttc
     1021 gcttacgcaa cgggacaacg cgatgctgaa cgggcgagtc atgcagttga gtcagcaggt
     1081 gcagcacttg agcgagcgga cagaacgctt gagccagtta tacagcgaga actggagata
     1141 agggatgagc ggctgataca ggagcgcgaa tatgcgttat ccctggagcg ggagcgccag
     1201 ccggaaattc aggaacggac actggatggc ccttcgctgg gatggtaacc agagacaggc
     1261 taaaatgttg gcagcgcgtt tccggtgcat tttttgggat aatggttggt tcgggggcac
     1321 cgggaacgcg cttcagtcag agcttgctga aaacacagga acgcgttaaa acgcatgcgg
     1381 gcgcgattct ggtgtgtctt tgttgctgtg ttttgccgtt attgccagat cgcgctcatt
     1441 cccatgaatt tctgtttttc cgctccggtt ccgtcttctg cctttccgaa agtatgaaaa
     1501 aaatccgttt cagtagtctc tggctggcaa ttcctgcgtg attcacatgg ctgcatagct
     1561 atgcatgtgg ttaaaattta tcgggtgcga tcgcggcagt ttttcgggtg gtttgttgcc
     1621 atttttacct gtctgctgcc gtgatcgcac cgaacgcgtt ttggcggtgc gtacaatgcg
     1681 acgttatggt aaatggagcg gattttcagg aaggagtgat actaacaact cgaacactgt
     1741 gcaaataatg attttttaaa atatcggtaa cgataataaa ttatttcgtt ttacgtcggt
     1801 aataaatata atgtagtttc agtatataaa aaatatatga ggtgtcttgt gggagctttc
     1861 atgaatcttc aacaacgtat aaataaacta ccacaattat catccagttt ttcatttgga
     1921 aaagatattg ataatattca ttcatttatc tttaacgaaa cttctaaaga taaaattgaa
     1981 gatttactaa gaaaatgggt gtcaggcaat cagccctgtg tatttggtaa attggcaagc
     2041 aagaaaatta aagggcttga tttccattta tctattgtaa atagccccca actatataat
     2101 gatgatggtc atttgtttga ttttttgcgt aatgaaagag ttcgttttaa agaacgcgca
     2161 agaagaggag aggtcagtgc gcatctcatt tattttattc atccacaact tgcatttgct
     2221 cgtccatcag aagagttagt ggacatacaa aaatatattt gttcgctgca tatgccagag
     2281 tgctatccaa taaaagagga tgttatttat actgaaagcg taccatttca ggataaagat
     2341 gggctaaaaa tatataaggc tggtgttaat gttttttact cttctgctca tagaacaaga
     2401 aatcatgata gaagaatacc ggggggaata cttatttctg taaatgcccc ggggcatttt
     2461 atgaggctgg cgatagaaaa aggtttctat aaagatcaag agcaagcgtt agcggatatt
     2521 cgtaacatga ctattcagtc tgttggtaac ggtgggtatt cacatccgga agggatttct
     2581 acaacatggc attcagaatc taaattggat cgttttggat gcccagtgca caccggtaat
     2641 agttcttatt acagcggttt ttatcatact gatgtattaa ttccaggaga attaactaag
     2701 gatgaaagat tgctccatga aatagataat agtgatccaa tgatcttcaa ctggaatgta
     2761 ttgttttatg tttcattaga agagtttcct atagatgatc cttattatgg agagtttatt
     2821 ggtgttcctg tagatgatgc atctatgttt tttaactcct ttcaaccaag gaaatttgaa
     2881 aacaaccccc tttatgagaa agaggatgat taatatgatt aaaggaatgc tttttgcttt
     2941 tttagcagcg ttctcatggg gggcagctat cgtaatgagc aaaaaaggag ttgaagaact
     3001 agatgccgga ggggtgtttt tctggcagat ttgtagtgca gtaatactat cttggatagt
     3061 tctttttata aaaagaaaaa aactcccatt aaactcaaaa gcgatacttg cttatgcgac
     3121 aggcgttttt gaaccattct tagcgtatac ttttacactc tacggacttt attttatttc
     3181 agcaggaagc gcatcaatta ttttttcact tgaatcagtg tttatattgg tgttgtctgt
     3241 tcttatttgc tcagttaaaa ttaattcccc gcaatatttt atattgttcc ttataggtgc
     3301 aattgttggc agtattttgg tagccttgcc agacatcagt aatggatcga ataatattgt
     3361 tggatattgt cttgttgttg ccggtgtgtt atcggcagca ttttatgttg ttatatcgtc
     3421 tcgccttatt aaatcgtttg agcctatcac attattgagc ggacagttga cattctccct
     3481 tatactgtca tcgctcttta tttttatatc taaatcctca atttatcttc cgttggaatc
     3541 cgtgattatt gttttttcat ctggaatatt acaatatttt ttagcttttt gtttttactt
     3601 gaactccttg cgttggctcc cagttcattt ggcagggata atgttgtact ttattccgct
     3661 agttgcatta tttttgtcat ggttgttttt ggatgaacaa ttcacattct tacagggggt
     3721 aggatttatt gtaactgtgc taagtgtata ttttcttaac agaaaatact cagaggagta
     3781 acattctgtt caggattgcc cccccggcct ccatcgatac cgtgccaaat gcacgtaaaa
     3841 aacgggagcg tttctggtgt cggaatgtaa ccaagggggg cagaggcgcg ctaaaacgct
     3901 ctcaggggag tcaaatagag acgtttgtaa caaaaagatc aaaggatcct cttgagatcc
     3961 tttttttctg cgcgtaatct gctgcctgta aacgaaaaaa ccgccttgca gggcggtttg
     4021 atcgaaggtt aagtcagctt gcagactgct taaccaggta actgtcttat gcgagacgag
     4081 tcaccaaaat tgttcttcta gtgtagccgc actccggcag ccacttcaag tactctctaa
     4141 atatctcgca catgactttt accaatggct gctgccagtg gcgctttgtg gtgtcttttc
     4201 cgggttggac ccaagagaac agttaccgga gaaggcgcag cagtcgggct gaacgggggg
     4261 ttcctgcata cagcccagcc cggagcgaac tgcctaagtc gccccgagat accagaagac
     4321 gtgagctatg agaaagcgcc acgtgtcccg taagggagaa aggcggaaaa ggtatccggt
     4381 aatcgatatg gcgggaacgt gggggcgcag gagggagcga ttcaccggaa acggtgatga
     4441 tctttaagcc acgtcgggtt tcgccaccgc tggcggattt catggttgag ccgcaaggct
     4501 cccacagatg caccgaaaaa gtgtctgttt tgtgaagtcc gtcagggggg cggagcctat
     4561 ggaaaaagcg cctccggcgc ggccttttcc ggggtttcgt tttttgcgca catgttcttt
     4621 cctggtttat ccccgggttc tgtggataac agggttagcg tttttgagaa aggagagtcc
     4681 gctcgccgca gccgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaagagcgc
     4741 ctgaactggc attttcgcct tacgcatctg tgcggcattt cacacccgac atggcgcact
     4801 tttcatacaa tccgcactga tgccgaatga ttaagccagt atacactccg ctatcgctac
     4861 gtgactgggt cagggcttcg ccccgacacc ccaaaggtca acggcctgac gcgccctggc
     4921 gggcttgtct gctcccggca tccgctcaca gacaagctgt gaccgtctcc gagagctgca
     4981 tgtgtcagcg gttttcaccg tcatccccga agcgtgcgag gcaactgcgg taaagctcat
     5041 cggcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agttcgttga
     5101 gcttctccag cagcgttaat gtctggcttc tgataaagcg gaccatgtta agggcggttt
     5161 tttcctgttt agtcactgga tgcctccgtg taagggggat ttctgttcat gggggtaatg
     5221 ataccgatga aacgcgagag gatgctcacg atacgggtga ctgatgatga gcatgcccgt
     5281 ttactggaac gctgtgaggg taaacaactg gcggcatgga tgcggcgggt ctgcctgggt
     5341 gagcctgttg cccgttccgg gaagctgccg acactggcac cgccgttact gcgtcagctg
     5401 gccgctatcg ggaataacct taaccagaca gcgaggaagg tgaacagcgg gcagtggtct
     5461 tccggtgacc gggttcaggt ggtggccgca ctgatagcca tcggggatga gctgcgccgg
     5521 ctgcgtctgg cggtcaggga acagggggcg cgggatgata gttaagtttc atgccagggg
     5581 aaaaggtggt ggcagtggtc cggttgatta cctgctggga agggagcgta accgggaagg
     5641 ggcaacggtg cttcagggga atccggaaga ggtccgggaa ctcatcgatg ccacgccatt
     5701 tgcgaagaaa tacacgtcag gtgttctgtc gttcgcggag aaggagctgc cgcctggagg
     5761 gcgtgaaaaa gtgatggcga gctttgagcg tgtactgatg cccggtctcg agaaaaatca
     5821 gtacagcatc ctgtgggtgg agcaccagga caagggacgg cttgagctga at
//
