LOCUS       NZ_OY754397             3918 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F719 plasmid pF719-5, complete sequence.
ACCESSION   NZ_OY754397
VERSION     NZ_OY754397.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542902
            Assembly: GCF_963575415.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 09:57:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,345
            CDSs (total)                      :: 5,215
            Genes (coding)                    :: 4,947
            CDSs (with protein)               :: 4,947
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 85 of 268
            Pseudo Genes (incomplete)         :: 196 of 268
            Pseudo Genes (internal stop)      :: 46 of 268
            Pseudo Genes (multiple problems)  :: 54 of 268
            Pseudo Genes (short protein)      :: 3 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754397.1.
            Complete genomic sequence of E. coli strain F719.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F719"
                     /db_xref="taxon:562"
                     /plasmid="pF719-5"
     gene            complement(join(2533..3918,1..165))
                     /locus_tag="R8803_RS26715"
                     /old_locus_tag="FGAF719_51430"
     CDS             complement(join(2533..3918,1..165))
                     /locus_tag="R8803_RS26715"
                     /old_locus_tag="FGAF719_51430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001624483.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKASI
                     PQTAGERKAALVALRSRWADVQNEHLARYGHESRVDHRSLKEQGINRTPEVHLGPVQAA
                     SLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPELLLQYR
                     KVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARYLDKLGR
                     NVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHERNEFRNKL
                     QQAESEWDKENAAFKRTEGYKYVGDLDRYREREILAAASLENTRQKVAEEVSVARSQML
                     SLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALRIQRSWAREASRSNERDQDMER"
                     /name="MobA/MobL family protein"
     gene            337..783
                     /locus_tag="R8803_RS26720"
                     /old_locus_tag="FGAF719_51410"
     CDS             337..783
                     /locus_tag="R8803_RS26720"
                     /old_locus_tag="FGAF719_51410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_021537414.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLAKDSA"
                     /name="conjugal transfer protein TraD"
     gene            complement(1045..2076)
                     /locus_tag="R8803_RS26725"
                     /old_locus_tag="FGAF719_51420"
     CDS             complement(1045..2076)
                     /locus_tag="R8803_RS26725"
                     /old_locus_tag="FGAF719_51420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_317721232.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSKNQADKGKVG
                     GKNSGVVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDSSGEWLAKKQI
                     IKY"
                     /name="replication initiation protein"
     Typing          complement(1301..2076)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.654"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(2537..3918)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.089"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcgggattt ttcttcgccc atgatggcat gttacctgat gacgaaaatt ccagatcctc
       61 ccgcgatttg tagctcttat attcgccgga tctggaaatg taatcagcat gttttaaggc
      121 actgcctttt gctccagctt tgacggtgca atggtagctt gccatctgcc ttttagccct
      181 ccgcaggagc gcacagtttc tcgacgaagg agagaaacta ctaagtgcgc atccctgaat
      241 taatcaggga tgattttacg gtctcacacg caggcagaca aggtgatatt atgcacaata
      301 acaactcaca ttaacggcaa aagtgcagag gtaagaatga caaatcgtga agagtggtta
      361 agtgcaaaaa ttgcctacat caacggactg aaatcaccgt cagagcagca gagattactt
      421 gtgttactgg cagagaaaaa aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg
      481 atacgggcag aaaaaaccgc agagaaagca gccgcagcaa aagccagggt aacggcaatc
      541 atagcggcag aacggaaggc agccgcaagg gcagaacgaa aggcgcggga tcatgaactc
      601 tacaaggccg caggcctcat gattgtggca ggactggtgg acagcaaaac cggaaaacca
      661 aagtttagtg ctgccgaact ggtaggcgca ctcgcaggaa tcgcagagct accgcgcaac
      721 catcccaaat ggcaggaatg ggaaaaacgc gggaaagagc tactcgccaa agacagtgcg
      781 taagagcctc cgttcacgtt ccggttccct ggtgcttcct cgctcattcg ctcgctacgc
      841 tcggtcatga gactgcggcg agcacaacca cctcaatcat gcggtaagcc gcaccattgc
      901 tgggctgaaa ggagaaaacc gccggaaagc ccgctgctca aaaagacgcc gaaaggcgct
      961 gttatctgat ataggcttgc ttcgtgtccg caaatttaac aacccattaa caaaaaccag
     1021 ggggtttgtt gctttttttg taaactaata tttaattatt tgtttctttg ctagccattc
     1081 tccagagcta tccatccagc gtcgaatagt ccgactgcta acaccaaccg ccactgcaat
     1141 atcttctgtt gacgctccca agatgtacat atcaagcgcc ctacgacgtt tatcatcgtt
     1201 agccgtccac cggacaacac cgctgttttt cccgccaact ttccctttgt ccgcctgatt
     1261 ttttgacgaa ttggccttac cgcccttgcg acctcgtgcg gcctgaattt ctggcgtgtg
     1321 cgtatcagcc acatactgtg cgaaagtttc cggcgtgaag ttcctgtgcg tgtatttcgc
     1381 aatgctcttg ccaatagccc gacattcagc cagtgaaagc ggaacgggaa gcgatgcatt
     1441 gtacatttcg acacgctgga tcaccgcttc aagccactgt gagaacgctg gccagccctg
     1501 acgaatcgcc ctgtaagccc atttgcgcgt catttcgaac aggtggcagt ttcgccccat
     1561 cccgtaatgt ttatcgatgc tacgacgctc tgaggcgctc aaatcgagat aatctgccag
     1621 ttcatcgagg gtataggcgt cctcgcgcca ctccatcacc tgccattcca ggtggaacgg
     1681 atttttgcaa atcaggccgc tgtagttcac atcagcacaa agtttctcac acagcgaacg
     1741 ttcaaccgct gcggcatatt tcagcgcctt aaccgaggca tcaggcgcgg ttctcacggc
     1801 gatgttgagt gcgtagagca ggtgagcgtg tccgttcaca ggatttttta cggtgatgtt
     1861 cggcgcgggg gcgttccggt cgctccagtc aatcgcagct cctacacggt caacgtcaaa
     1921 aaccagccag aactgcgcat gaggctggtt ctgctggatg taccgcgcaa gaagcgcacg
     1981 ccctttgccg gagatgcgaa gaccaaaagc gagatcatcc gtgtgatagg gacggtgggg
     2041 taaattttct tcgaagtatt gaagcgcggc gctcatgtgt taggatttcc tccgtgtgtt
     2101 acgacccaca cgccccttga attttcgtga caatcttggc ggagagacac gaatttcagg
     2161 ggcgtttgtt tttcagtcgc ttacactacc cccaacgaca aaacctgtcg agccttcccg
     2221 caaaaccgat ccatttacca taactcccgt tgtacgtacc ccgtaaaacg ccctcagtcg
     2281 cttcctgacg caaaaaacgc aaaaagacag gaaaacacca ggccaaacca gaaaaacgcg
     2341 tcagaatcgt tttcagagcg ttgcataact atgcacgaaa gcgatgcgcg atctgtttcg
     2401 agcgttcaga agggcgaacg ggtcaaaaca gcaaactttc tcatcagaaa acggtgaacg
     2461 ggacgcggaa ttttttcgcg ttccgtgaaa cgttggcggg gttcggggcg cagtcccgat
     2521 attgctacat tttcaacgct ccatatcttg atcacgttcg ttactacgtg atgcctccct
     2581 ggcccaggat cgctgtatac gcagtgcctt ttcctcctgt tgctgccgtt cctgcgccat
     2641 ttctgccagt aactcatgtc gcatttgagc tttttcatct ccagatatac tgagttcagg
     2701 ctccagagat aacatctggc tacgggcaac ggatacttct tcagcaacct tttgcctggt
     2761 attctctaaa gatgccgcgg ccagaatctc acgttctctg tacctgtcca gatccccaac
     2821 gtacttatat ccctcggtac gcttaaaagc agcgttttct ttgtcccact cggattcggc
     2881 ttgctgtagc ttgttcctga attcattcct ttcatgttca gcggtttcca gcacactttt
     2941 ttgctccaga atacgcgcat caatctccct tttgtcagcc ccccgaaaca ggccacgaat
     3001 cccattcaaa gcattgcgtt gttcctgtag ctcccgcagc tccctttgtg cggaaacgac
     3061 attacgcccc agtttgtcta aataccgtgc cccttcatat attcgcctgc tccattcgga
     3121 aagagagtct ttttttgctt tcgcattctg aagcagctta tgttctttga gcgcattggg
     3181 attggcatcc cctaaacgag caagccttgc ttctccctga atgaccgttt tcataacctt
     3241 gcggtactga agcaataact ccggtgaacg ggctgaacga acaggctcaa ctgccttgat
     3301 tttttgcttt tgctcctgtt cctgctgaat ggcatttgcg gcatctctgg cggtcttgag
     3361 ttcccgctcg gcattcctcc gttcctggat tgcaacaatc tgctcaccat tgagactggc
     3421 ggcctgaacg gggccaagat gaacttccgg cgtgcgattg ataccctgtt ctttcagaga
     3481 acgatgatct acgcgcgact cgtgaccgta tcgcgcaagg tgctcattct gcacatctgc
     3541 ccagcgggaa cgcagcgcaa caagggcggc ttttcgctct ccggctgtct ggggaatact
     3601 cgcttttttt gcgcctcccc gttctggtga tttgctgttg gcacgtttaa aaaattgctg
     3661 cggatcgcgt tcaataccat cattaatccg ctcgcaaaac atgatatgcg cgtgtggctg
     3721 ttcctttccg tcgattgcac ctggcggatt gtggatcgcg tactggtagg catggcgatc
     3781 gccaagttcc ttttgcacga aatcttcaac aagttctatc cgttgttcgc gtgtcagttc
     3841 acgcggtaac gcgatctcaa tttcacggta tgcagtcccg ttttttcgtt caaattcatc
     3901 tgcggctttc catagctc
//
